Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,458,650 +G intergenic (‑161/+20) b2342 ← / ← b2343 putative acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,458,6461.G99.4% 583.1 / ‑3.9 180intergenic (‑157/+24)b2342/b2343putative acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (104/75);  total (104/76)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 199 total aligned reads displayed.

CATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAG  >  NC_000913/2458555‑2458735
                                                                                            |                                                                                         
cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g                                                                                          <  1:4159594/93‑1 (MQ=255)
cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g                                                                                          >  2:3291129/1‑93 (MQ=255)
 aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑gg                                                                                         <  2:2160203/93‑1 (MQ=255)
 aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑gg                                                                                         >  1:2556781/1‑93 (MQ=255)
 aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑gg                                                                                         >  1:1817214/1‑93 (MQ=255)
   ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑gggg                                                                                       <  1:2985950/93‑1 (MQ=255)
    tAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑ggggg                                                                                      >  2:2420658/1‑92 (MQ=255)
      gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑gggggcg                                                                                    >  1:183887/1‑90 (MQ=255)
         aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtac                                                                                  >  1:6519737/1‑93 (MQ=255)
         aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtac                                                                                  >  1:3252653/1‑93 (MQ=255)
          aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtacg                                                                                 >  1:6584924/1‑93 (MQ=255)
          aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtacg                                                                                 >  1:5260880/1‑93 (MQ=255)
          aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtacg                                                                                 >  1:3084130/1‑93 (MQ=255)
           gaggAAAAAAGGAAAGGTGGGACCTATAAAAAAGAGAGAAAGAAAAAAAAAAAAAAAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtacgt                                                                                <  1:5725753/91‑1 (MQ=255)
            tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGcgtacgta                                                                               >  2:4725853/1‑93 (MQ=255)
                gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCg                                                                           >  1:2418383/1‑93 (MQ=255)
                   aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCgaga                                                                        >  2:5774437/1‑93 (MQ=255)
                   aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCgaga                                                                        <  2:3901034/93‑1 (MQ=255)
                    aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGagag                                                                       >  1:10654/1‑93 (MQ=255)
                    aGGGAAAGGGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGagag                                                                       <  2:1696154/93‑1 (MQ=255)
                       gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGaa                                                                    <  1:1990770/93‑1 (MQ=255)
                       gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGaa                                                                    <  1:1652371/93‑1 (MQ=255)
                       gAAAGTGGGAGCTATGACACAGAGAAAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGaa                                                                    <  2:290546/93‑1 (MQ=255)
                        aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAAt                                                                   <  1:565810/93‑1 (MQ=255)
                         aaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAAtt                                                                  >  2:5794239/1‑93 (MQ=255)
                          aGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTa                                                                 >  1:4355093/1‑93 (MQ=255)
                           gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTaa                                                                <  1:2114889/93‑1 (MQ=255)
                           gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTaa                                                                >  2:6276492/1‑93 (MQ=255)
                            ggggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAAc                                                               <  1:745429/92‑1 (MQ=255)
                               gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                            >  2:540488/1‑93 (MQ=255)
                               gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                            >  2:1358649/1‑93 (MQ=255)
                               gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                            >  2:111125/1‑93 (MQ=255)
                                 gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                          <  1:1019949/93‑1 (MQ=255)
                                 gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                          >  1:1495351/1‑93 (MQ=255)
                                 gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                          >  2:5530577/1‑93 (MQ=255)
                                  cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                         <  2:2789858/93‑1 (MQ=255)
                                   tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                        >  1:6146867/1‑93 (MQ=255)
                                    aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTATCCGAGAGGAATTAACGCAGAccc                                                       >  1:3293583/1‑93 (MQ=255)
                                    aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                       >  1:4553421/1‑93 (MQ=255)
                                    aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                       <  2:696326/93‑1 (MQ=255)
                                     tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                      <  1:4239164/93‑1 (MQ=255)
                                     tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                      <  2:6517533/93‑1 (MQ=255)
                                       acacAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCACACCCAGc                                                    >  1:3927023/1‑93 (MQ=255)
                                       acacAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                                    <  1:6520922/93‑1 (MQ=255)
                                        cacaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                   >  2:5467211/1‑93 (MQ=255)
                                        cacaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                   <  1:639666/93‑1 (MQ=255)
                                          caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTgg                                                 >  1:3734671/1‑93 (MQ=255)
                                          aagagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTgg                                                 <  2:4921998/92‑1 (MQ=255)
                                              gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                             >  1:903528/1‑93 (MQ=255)
                                              gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                             >  2:865729/1‑93 (MQ=255)
                                                 aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                          <  2:2016467/93‑1 (MQ=255)
                                                   aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGc                                        >  1:4202270/1‑93 (MQ=255)
                                                    agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                       >  1:2652292/1‑93 (MQ=255)
                                                    agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                       >  1:564191/1‑93 (MQ=255)
                                                    agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                       <  2:599162/93‑1 (MQ=255)
                                                     aaaaaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                      <  2:3640446/89‑1 (MQ=255)
                                                      aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                     <  1:5490822/93‑1 (MQ=255)
                                                        gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                   >  2:4170545/1‑93 (MQ=255)
                                                        gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                   >  2:2825503/1‑93 (MQ=255)
                                                        gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                   >  2:1775874/1‑93 (MQ=255)
                                                          agagAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                 >  2:2703146/1‑93 (MQ=255)
                                                           gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                >  1:3352649/1‑93 (MQ=255)
                                                           gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                >  2:3976545/1‑93 (MQ=255)
                                                            agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATGAGCATTTCAGCt                               <  1:1148219/93‑1 (MQ=255)
                                                            agaaaaaaaaACGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                               <  2:3601099/93‑1 (MQ=255)
                                                              aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTGCCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                             >  2:3572905/1‑93 (MQ=255)
                                                              aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                             <  2:3277473/93‑1 (MQ=255)
                                                               aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                            >  1:3891572/1‑93 (MQ=255)
                                                                 agaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                          >  2:1300511/1‑93 (MQ=255)
                                                                 agaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                          >  1:846900/1‑93 (MQ=255)
                                                                  gaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                         >  2:4385516/1‑93 (MQ=255)
                                                                  gaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                         <  1:4820875/93‑1 (MQ=255)
                                                                  aaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                         <  1:4677783/92‑1 (MQ=255)
                                                                  aaaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                         <  2:3698693/92‑1 (MQ=255)
                                                                   aaaaCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                        >  1:3869106/1‑93 (MQ=255)
                                                                     aaCGCCCCTGCCAGCAACTGACAGGGGGCTTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCa                      <  2:747772/93‑1 (MQ=255)
                                                                      aCGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                     >  2:5178254/1‑93 (MQ=255)
                                                                       cGCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaa                    >  2:459515/1‑93 (MQ=255)
                                                                        gCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                   <  1:2695033/93‑1 (MQ=255)
                                                                        gCCCCTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                   <  1:3774802/93‑1 (MQ=255)
                                                                        cccccTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                   <  2:5387368/92‑1 (MQ=255)
                                                                         ccccTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                  <  1:6522167/93‑1 (MQ=255)
                                                                          cccTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                 >  1:2947134/1‑93 (MQ=255)
                                                                          cccTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                 >  1:6249528/1‑93 (MQ=255)
                                                                          cccTGCCAGCAACTCACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                 >  1:5942400/1‑93 (MQ=255)
                                                                            cTGCCAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa               <  2:1959796/93‑1 (MQ=255)
                                                                               ccAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt            <  1:3575895/93‑1 (MQ=255)
                                                                                cAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc           >  1:6298443/1‑93 (MQ=255)
                                                                                cAGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc           <  2:1470845/93‑1 (MQ=255)
                                                                                 aGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg          >  2:4636423/1‑93 (MQ=255)
                                                                                 aGCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg          >  1:1761380/1‑93 (MQ=255)
                                                                                  gCAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa         <  2:5826434/93‑1 (MQ=255)
                                                                                   cAACTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat        <  2:3100414/93‑1 (MQ=255)
                                                                                    aaCTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata       >  1:4277563/1‑93 (MQ=255)
                                                                                    aaCTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata       <  2:6631136/93‑1 (MQ=255)
                                                                                     aCTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat      <  2:1789300/93‑1 (MQ=255)
                                                                                      cTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc     >  2:5685729/1‑93 (MQ=255)
                                                                                      cTGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc     <  2:3335217/93‑1 (MQ=255)
                                                                                       tGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc    <  1:5180995/93‑1 (MQ=255)
                                                                                       tGACAGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc    <  1:3504273/93‑1 (MQ=255)
                                                                                            |                                                                                         
CATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAG  >  NC_000913/2458555‑2458735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACAC  >  NC_000913/2458558‑2458743
                                                                                             |                                                                                              
TTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGG‑G                                                                                              <  SRR4302536.6415191/94‑1 (MQ=60)
TTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGG‑G                                                                                              <  SRR4302536.3073819/94‑1 (MQ=60)
TTAGCCAAGCGGAGAAAAGGAAAAGCGGCATCAATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGG‑G                                                                                              <  SRR4302536.6132106/94‑1 (MQ=60)
 tagacaagagaagaaaagaaaacgtggaatcaacgacagagaaaaaaaaaAAGAAGAAAAAAAAAAACGCCCCTGCCAGCAACTGACA‑GGGGgc                                                                                              <  SRR4302536.2457551/44‑3 (MQ=60)
  AGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGGgcg                                                                                             >  SRR4302536.188742/1‑91 (MQ=60)
    caaagggaAGAAAAGGAAAAGTGGGAGCTATGACAAAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTA                                                                                           <  SRR4302536.112893/86‑1 (MQ=60)
    CCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTA                                                                                           >  SRR4302536.6867680/1‑94 (MQ=60)
     CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTAC                                                                                          >  SRR4302536.3348006/1‑94 (MQ=60)
     CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTAC                                                                                          >  SRR4302536.6713958/1‑94 (MQ=60)
     CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTAC                                                                                          <  SRR4302536.1940761/94‑1 (MQ=60)
      AAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACG                                                                                         >  SRR4302536.1736720/1‑94 (MQ=60)
      AAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACG                                                                                         >  SRR4302536.6780839/1‑94 (MQ=60)
      AAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACG                                                                                         >  SRR4302536.5417662/1‑94 (MQ=60)
      AAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACG                                                                                         >  SRR4302536.3174353/1‑94 (MQ=60)
       AGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGGgggtacgt                                                                                        <  SRR4302536.5754839/94‑9 (MQ=60)
        gaggaaaaaaggaaaggtgggacctataaaaaagagagaaagaaaaaaaaaaaaaaAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTA                                                                                       <  SRR4302536.5896034/38‑1 (MQ=56)
        GTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTA                                                                                       >  SRR4302536.3003158/1‑94 (MQ=60)
         TGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTAC                                                                                      >  SRR4302536.2556124/1‑94 (MQ=60)
          GGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACC                                                                                     >  SRR4302536.6713971/1‑94 (MQ=60)
           GAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCC                                                                                    <  SRR4302536.791977/94‑1 (MQ=60)
            AGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCG                                                                                   >  SRR4302536.3380986/1‑94 (MQ=60)
            AGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCG                                                                                   >  SRR4302536.2491531/1‑94 (MQ=60)
              AAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGAGAGGGGG‑CGTACGTACCCGAG                                                                                 <  SRR4302536.4294080/94‑1 (MQ=60)
               AAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGGGA                                                                                <  SRR4302536.6785415/94‑1 (MQ=60)
               AAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGA                                                                                >  SRR4302536.4793070/1‑94 (MQ=60)
                AAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAG                                                                               >  SRR4302536.6040162/1‑94 (MQ=60)
                AAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAG                                                                               >  SRR4302536.10958/1‑94 (MQ=60)
                 AGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGG                                                                              >  SRR4302536.1839167/1‑94 (MQ=60)
                 AGGAAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAAAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGG                                                                              <  SRR4302536.3853557/94‑1 (MQ=60)
                  GGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGA                                                                             >  SRR4302536.582092/1‑94 (MQ=60)
                    GAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAAT                                                                           <  SRR4302536.2050628/94‑1 (MQ=60)
                    GAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAAT                                                                           <  SRR4302536.1700392/94‑1 (MQ=60)
                     AAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATT                                                                          <  SRR4302536.580219/94‑1 (MQ=60)
                      AAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTA                                                                         >  SRR4302536.4485359/1‑94 (MQ=60)
                        GTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAAC                                                                       <  SRR4302536.2178437/94‑1 (MQ=60)
                        GTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAAC                                                                       <  SRR4302536.1540084/94‑1 (MQ=60)
                         GGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACG                                                                      <  SRR4302536.767325/94‑1 (MQ=60)
                          ggagataacaaaacagaagaAAAAGAAGAAGAAAAAAAAGAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGC                                                                     <  SRR4302536.3577400/74‑1 (MQ=60)
                          GGGAGCTATGACACAGAGAGATAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGC                                                                     >  SRR4302536.6648720/1‑94 (MQ=60)
                             AGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGA                                                                  >  SRR4302536.1539499/1‑94 (MQ=60)
                              GCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGAC                                                                 <  SRR4302536.1050142/94‑1 (MQ=60)
                               CTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACC                                                                >  SRR4302536.6329999/1‑94 (MQ=60)
                                TATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTATCCGAGAGGAATTAACGCAGACCC                                                               >  SRR4302536.3390564/1‑94 (MQ=60)
                                TATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCC                                                               >  SRR4302536.4689996/1‑94 (MQ=60)
                                  TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAG                                                             >  SRR4302536.322288/1‑94 (MQ=60)
                                  TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAG                                                             >  SRR4302536.2026307/1‑94 (MQ=60)
                                  TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAG                                                             <  SRR4302536.4365371/94‑1 (MQ=60)
                                   TACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCACACCCAGC                                                            >  SRR4302536.4043710/1‑94 (MQ=60)
                                    ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCT                                                           <  SRR4302536.6715172/94‑1 (MQ=60)
                                    ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCT                                                           >  SRR4302536.946605/1‑94 (MQ=60)
                                    ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCT                                                           <  SRR4302536.354631/94‑1 (MQ=60)
                                     CACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTG                                                          <  SRR4302536.658546/94‑1 (MQ=60)
                                      ACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGG                                                         >  SRR4302536.3846289/1‑94 (MQ=60)
                                       CAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGA                                                        >  SRR4302536.4337737/1‑94 (MQ=60)
                                          AGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAA                                                     >  SRR4302536.930150/1‑94 (MQ=60)
                                             GAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCA                                                  <  SRR4302536.1107524/94‑1 (MQ=60)
                                               AAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGC                                                >  SRR4302536.4327184/1‑94 (MQ=60)
                                                AAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCA                                               >  SRR4302536.2731718/1‑94 (MQ=60)
                                                AAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCA                                               >  SRR4302536.578558/1‑94 (MQ=60)
                                                 agaaaaaaaaaaaaaAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAT                                              <  SRR4302536.5814255/79‑1 (MQ=60)
                                                   AAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTT                                            <  SRR4302536.5654708/94‑1 (MQ=60)
                                                      AAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAG                                         <  SRR4302536.2231838/94‑1 (MQ=60)
                                                       AGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGC                                        >  SRR4302536.3452121/1‑94 (MQ=60)
                                                        GAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCT                                       >  SRR4302536.2764027/1‑94 (MQ=60)
                                                         AGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATGAGCATTTCAGCTT                                      <  SRR4302536.1182741/94‑1 (MQ=60)
                                                           AAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCG                                    >  SRR4302536.4007285/1‑94 (MQ=60)
                                                             AAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                                  >  SRR4302536.2656627/1‑94 (MQ=60)
                                                             AAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                                  >  SRR4302536.871807/1‑94 (MQ=60)
                                                             AAGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                                  >  SRR4302536.6144992/1‑94 (MQ=60)
                                                              AGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                                 >  SRR4302536.3448438/1‑94 (MQ=60)
                                                              AGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                                 >  SRR4302536.6548474/1‑94 (MQ=60)
                                                              AGAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                                 <  SRR4302536.3088221/94‑1 (MQ=60)
                                                               GAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                                <  SRR4302536.4965951/94‑1 (MQ=60)
                                                               GAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                                >  SRR4302536.3984223/1‑94 (MQ=60)
                                                               GAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                                >  SRR4302536.4417692/1‑94 (MQ=60)
                                                               AAAAACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                                <  SRR4302536.4818455/94‑1 (MQ=60)
                                                                  AACGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAA                             >  SRR4302536.1884426/1‑94 (MQ=60)
                                                                    CGCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAA                           >  SRR4302536.2149507/1‑94 (MQ=60)
                                                                     GCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAG                          <  SRR4302536.3887473/94‑1 (MQ=60)
                                                                     GCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAG                          <  SRR4302536.2775477/94‑1 (MQ=60)
                                                                     CCCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAG                          <  SRR4302536.3678007/94‑1 (MQ=60)
                                                                      CCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                         <  SRR4302536.6716459/94‑1 (MQ=60)
                                                                      CCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                         >  SRR4302536.6435710/1‑94 (MQ=60)
                                                                      CCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                         <  SRR4302536.3036654/94‑1 (MQ=60)
                                                                      CCCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                         >  SRR4302536.3034010/1‑94 (MQ=60)
                                                                      CCCCTGCCAGCAACTCACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                         >  SRR4302536.6119439/1‑94 (MQ=60)
                                                                       CCCTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                        >  SRR4302536.6450955/1‑94 (MQ=60)
                                                                         CTGCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA                      <  SRR4302536.5557484/94‑1 (MQ=60)
                                                                           GCCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAT                    >  SRR4302536.1272907/1‑94 (MQ=60)
                                                                            CCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC                   >  SRR4302536.6486073/1‑94 (MQ=60)
                                                                            CCAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC                   <  SRR4302536.3682812/94‑1 (MQ=60)
                                                                            CCAGCAACTAACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC                   >  SRR4302536.6748579/1‑94 (MQ=60)
                                                                             CAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG                  >  SRR4302536.1814334/1‑94 (MQ=60)
                                                                             CAGCAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG                  <  SRR4302536.4009142/94‑1 (MQ=60)
                                                                             CAGCAACTGACAGGGGG‑CGGACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGAGAAATCG                  >  SRR4302536.4127763/1‑94 (MQ=60)
                                                                                CAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATA               >  SRR4302536.4405090/1‑94 (MQ=60)
                                                                                CAACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATA               <  SRR4302536.5766693/94‑1 (MQ=60)
                                                                                 AACTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATAT              <  SRR4302536.3993912/94‑1 (MQ=60)
                                                                                   CTGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCC            >  SRR4302536.4922661/1‑94 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           <  SRR4302536.3608855/94‑1 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           <  SRR4302536.5365996/94‑1 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           <  SRR4302536.3808741/94‑1 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           <  SRR4302536.5335537/94‑1 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           >  SRR4302536.3872818/1‑94 (MQ=60)
                                                                                    TGACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA           >  SRR4302536.4460264/1‑94 (MQ=60)
                                                                                      ACAGGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGG         <  SRR4302536.1356666/94‑1 (MQ=60)
                                                                                      ACA‑GGGGTCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGG         <  SRR4302536.389396/94‑1 (MQ=60)
                                                                                        agGGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCG       <  SRR4302536.1264654/92‑1 (MQ=60)
                                                                                           GGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCAC    >  SRR4302536.3564650/1‑94 (MQ=60)
                                                                                             G‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACAC  >  SRR4302536.6456811/1‑94 (MQ=60)
                                                                                             |                                                                                              
TTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑GGGG‑CGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACAC  >  NC_000913/2458558‑2458743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑