Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,405,469 T→C W76R (TGG→CGG)  b3258 → sodium/pantothenate symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,405,4690TC100.0% 585.7 / NA 181W76R (TGG→CGG) b3258sodium/pantothenate symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (67/114);  total (67/114)

Only 100 of 228 total aligned reads displayed.

ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558
                                                                                          |                                                                                         
  tgtgCTGGCGATGTGGCTCCCCGCGAGCTATATCAGTGCCAGTTCGTTTATCGGCGTGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGt                                                                                       <  2:1528367/93‑1 (MQ=255)
  tgtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGt                                                                                       <  1:1277476/93‑1 (MQ=255)
  tgtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGt                                                                                       <  2:5450161/93‑1 (MQ=255)
   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  2:1015863/93‑1 (MQ=255)
   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGACACGAGCTGCTTATAAATACGGGctgggccgggga                                                                                      >  1:2172692/1‑91 (MQ=255)
    tgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAt                                                                                     <  2:2132946/93‑1 (MQ=255)
     tcTGGCGATGGCGCTTTCGGCGTCCTATATCAGTGCCGGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAtt                                                                                    <  1:1867108/92‑1 (MQ=255)
     gCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAtt                                                                                    <  1:3107083/93‑1 (MQ=255)
       tGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGc                                                                                  <  1:3777050/93‑1 (MQ=255)
        ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCt                                                                                 <  1:817606/93‑1 (MQ=255)
        ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTCCTTATAAATACGGGCTGGGGCGGGGATTTCt                                                                                 >  2:3713902/1‑93 (MQ=255)
         gCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTg                                                                                <  2:5253642/93‑1 (MQ=255)
          cGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTgg                                                                               <  1:1572823/93‑1 (MQ=255)
          cGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTgg                                                                               <  2:3583181/93‑1 (MQ=255)
          cGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTgg                                                                               >  2:3171576/1‑93 (MQ=255)
             tGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTTGGCCGGGGATTGCTCGCga                                                                            >  2:6673384/1‑93 (MQ=255)
              gACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgat                                                                           <  1:5550488/93‑1 (MQ=255)
               aCGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgatg                                                                          <  1:6011813/93‑1 (MQ=255)
                cGCTCACCGCGACTTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgatga                                                                         <  1:2715702/93‑1 (MQ=255)
                 gCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgatgat                                                                        <  1:1164356/93‑1 (MQ=255)
                 gCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgatgat                                                                        <  1:1062203/93‑1 (MQ=255)
                  cTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGAtt                                                                       <  2:2826949/93‑1 (MQ=255)
                   tCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTc                                                                      <  2:5056642/93‑1 (MQ=255)
                   tCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTc                                                                      >  1:2852824/1‑93 (MQ=255)
                    cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCa                                                                     <  2:4434228/93‑1 (MQ=255)
                      ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGc                                                                   <  1:258741/93‑1 (MQ=255)
                      ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGc                                                                   <  2:5721949/93‑1 (MQ=255)
                      ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGc                                                                   <  2:6356953/93‑1 (MQ=255)
                      ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGc                                                                   >  2:1974198/1‑93 (MQ=255)
                       cgcgACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCt                                                                  <  1:4027216/93‑1 (MQ=255)
                       cgcgACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCt                                                                  <  1:4128095/93‑1 (MQ=255)
                          gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTcc                                                               <  1:1265956/93‑1 (MQ=255)
                           aCCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTTGGGGTGGTTCAGCTTCCt                                                              >  1:4805358/1‑93 (MQ=255)
                           aCCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCt                                                              <  1:2262252/93‑1 (MQ=255)
                            ccTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGAGGAGTCAGCTTCCTg                                                             >  2:4902535/1‑93 (MQ=255)
                            ccTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTg                                                             <  2:790296/93‑1 (MQ=255)
                            ccTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTg                                                             <  2:4325214/93‑1 (MQ=255)
                             cTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGATTCAGCTTCCTGc                                                            >  2:2296129/1‑93 (MQ=255)
                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGATTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCa                                                           >  1:5433284/1‑93 (MQ=255)
                               atatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAg                                                          <  1:1520611/93‑1 (MQ=255)
                               atatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAg                                                          <  2:155743/93‑1 (MQ=255)
                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        <  2:1855394/93‑1 (MQ=255)
                                  tCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCt                                                       >  2:2250347/1‑93 (MQ=255)
                                  tCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCt                                                       <  2:5844798/93‑1 (MQ=255)
                                      tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCt                                                   <  2:1663930/93‑1 (MQ=255)
                                      tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTAAGCTTCCTGCAGTCTGGCt                                                   <  1:6572592/93‑1 (MQ=255)
                                       gctaGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCtt                                                  <  1:395788/90‑1 (MQ=255)
                                       gCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCtt                                                  <  2:5285615/93‑1 (MQ=255)
                                        ccAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt                                                 <  2:2422821/93‑1 (MQ=255)
                                         cAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTc                                                <  1:6181372/93‑1 (MQ=255)
                                           gTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc                                              <  1:2199998/93‑1 (MQ=255)
                                           gTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc                                              >  2:5334783/1‑93 (MQ=255)
                                            ttCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt                                             >  2:6243933/1‑93 (MQ=255)
                                            ttCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt                                             >  2:1069581/1‑93 (MQ=255)
                                               gTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCgg                                          <  2:2165540/93‑1 (MQ=255)
                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGt                                         <  2:4978060/93‑1 (MQ=255)
                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGt                                         <  2:3919583/93‑1 (MQ=255)
                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGt                                         <  1:4187454/93‑1 (MQ=255)
                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTTACTCGGt                                         >  1:3670733/1‑93 (MQ=255)
                                                 ttATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTTGGCCGGGGGTTGCTGGCGATGATTCCGCTTTCTGCAGGCTGGCTTTCACTCCGtg                                        >  1:3620288/1‑92 (MQ=255)
                                                 ttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTa                                        <  2:5781757/93‑1 (MQ=255)
                                                    tCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtc                                     <  1:198717/93‑1 (MQ=255)
                                                    tCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtc                                     <  2:648561/93‑1 (MQ=255)
                                                    tCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtc                                     <  1:1781264/93‑1 (MQ=255)
                                                      ggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCGCTCGGTATtctc                                   <  1:5054111/93‑1 (MQ=255)
                                                          ggCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCa                               >  2:2921335/1‑93 (MQ=255)
                                                          ggCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCa                               >  2:1496843/1‑93 (MQ=255)
                                                           gCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaa                              >  1:3246888/1‑93 (MQ=255)
                                                           gCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaa                              >  1:5902611/1‑93 (MQ=255)
                                                            ccAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaag                             <  2:4663144/93‑1 (MQ=255)
                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            >  2:4461111/1‑93 (MQ=255)
                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            <  2:3335447/93‑1 (MQ=255)
                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            >  1:4548752/1‑93 (MQ=255)
                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            <  2:2024787/93‑1 (MQ=255)
                                                              aGGAGTTGCTTATAAATGCGGGCTGGTTCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaa                           <  1:5118943/93‑1 (MQ=255)
                                                               ggAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaag                          <  1:4733728/93‑1 (MQ=255)
                                                                gAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGt                         >  1:6351065/1‑93 (MQ=255)
                                                                 aGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt                        >  2:2781683/1‑93 (MQ=255)
                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     <  1:2421587/93‑1 (MQ=255)
                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     >  1:5672950/1‑93 (MQ=255)
                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     <  1:2918427/93‑1 (MQ=255)
                                                                     gctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCg                    >  1:6038693/1‑93 (MQ=255)
                                                                      ctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGa                   >  1:6507912/1‑93 (MQ=255)
                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  <  1:5004543/93‑1 (MQ=255)
                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATGCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  >  2:4818065/1‑93 (MQ=255)
                                                                        tataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAtt                 <  1:4361399/93‑1 (MQ=255)
                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                <  2:4080729/93‑1 (MQ=255)
                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                >  2:3654538/1‑93 (MQ=255)
                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                <  2:6047280/93‑1 (MQ=255)
                                                                           aaaTACGGGCTGGGTCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCtt              <  2:749134/93‑1 (MQ=255)
                                                                           aaaTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCtt              <  1:6356966/93‑1 (MQ=255)
                                                                            aaTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGATTCCTGCAGTCTGGCTTTCCCTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTg             <  2:4139860/93‑1 (MQ=255)
                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  1:2109703/93‑1 (MQ=255)
                                                                              tACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcg           >  2:2636503/1‑93 (MQ=255)
                                                                                cGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGGAGTTTGCGATTCTTgcgcg         >  1:1763372/1‑93 (MQ=255)
                                                                                   gctgggccggGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg      >  2:306449/1‑93 (MQ=255)
                                                                                    ctgggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccgc     <  2:2578771/93‑1 (MQ=255)
                                                                                     cgggccgggTACTGCTGGCAATGATTCAGCTACCTGCAGTCTGGCTTTCACCCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCt    <  1:1849976/92‑1 (MQ=255)
                                                                                      gggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTa   >  2:1751328/1‑93 (MQ=255)
                                                                                      gggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTa   <  2:3034427/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562
                                                                                             |                                                                                             
GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATAACGGCTGGGCC                                                                                               >  SRR4302536.771139/1‑94 (MQ=60)
 GTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCTTTTAAATACGGGGTGGGCCG                                                                                              >  SRR4302536.183623/1‑94 (MQ=60)
  TATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGG                                                                                             >  SRR4302536.2341386/1‑94 (MQ=60)
     TGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTA                                                                                          <  SRR4302536.4562979/94‑1 (MQ=60)
     TGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTA                                                                                          <  SRR4302536.1315727/94‑1 (MQ=60)
     TGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGACACGAGCTGCTTATAAATACGGGCTGGGCCGGGGA                                                                                          >  SRR4302536.2238121/1‑94 (MQ=60)
      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAT                                                                                         <  SRR4302536.4353224/94‑1 (MQ=60)
      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAT                                                                                         <  SRR4302536.2167478/94‑1 (MQ=60)
        tctggcgatggcgctttcggcgtCCTATATCAGTGCCGGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTG                                                                                       <  SRR4302536.1923308/71‑1 (MQ=60)
        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTTAATACGGGCTGGGCCGGGTATTG                                                                                       >  SRR4302536.1264491/1‑94 (MQ=60)
        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTG                                                                                       <  SRR4302536.3857252/94‑1 (MQ=60)
        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTG                                                                                       <  SRR4302536.3197791/94‑1 (MQ=60)
        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTG                                                                                       <  SRR4302536.5119605/94‑1 (MQ=60)
          TGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCT                                                                                     <  SRR4302536.3889777/94‑1 (MQ=60)
          TGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCT                                                                                     <  SRR4302536.1906319/94‑1 (MQ=60)
           GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTG                                                                                    <  SRR4302536.1565608/94‑1 (MQ=60)
           GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTG                                                                                    <  SRR4302536.841636/94‑1 (MQ=60)
             CGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGC                                                                                  <  SRR4302536.1618939/94‑1 (MQ=60)
                 GACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATG                                                                              <  SRR4302536.5715842/94‑1 (MQ=60)
                  ACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGA                                                                             <  SRR4302536.6191037/94‑1 (MQ=60)
                   ccaTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGAT                                                                            <  SRR4302536.5601680/91‑1 (MQ=60)
                   CGCTCACCGCGACTTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGAT                                                                            <  SRR4302536.2796700/94‑1 (MQ=60)
                    GCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATT                                                                           <  SRR4302536.6918369/94‑1 (MQ=60)
                    GCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATT                                                                           <  SRR4302536.1093972/94‑1 (MQ=60)
                    GCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATT                                                                           <  SRR4302536.1199401/94‑1 (MQ=60)
                     CTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTC                                                                          >  SRR4302536.2937155/1‑94 (MQ=60)
                       CACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGCCCGGGATTGCTGGCGATGATTCAG                                                                        >  SRR4302536.4056828/1‑94 (MQ=60)
                        ACCGCGCCCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCGGGGCCGGGTATTGCTGGCGATGATTCAGC                                                                       <  SRR4302536.1308729/94‑1 (MQ=60)
                         CCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCT                                                                      <  SRR4302536.265649/94‑1 (MQ=60)
                         CCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCT                                                                      <  SRR4302536.6749124/94‑1 (MQ=60)
                          CGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTT                                                                     <  SRR4302536.4250475/94‑1 (MQ=60)
                          CGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTT                                                                     <  SRR4302536.4146665/94‑1 (MQ=60)
                           GCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCCTATAAATACGGGCTCGGTCGGGTATTGCTGGCGgtgggttagcgtc                                                                    >  SRR4302536.5576151/1‑81 (MQ=60)
                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTTGGGGTGGTTCAGCTTCCT                                                                  >  SRR4302536.4949988/1‑94 (MQ=60)
                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  <  SRR4302536.229400/94‑1 (MQ=60)
                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  <  SRR4302536.1303901/94‑1 (MQ=60)
                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCACGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCCTCCT                                                                  >  SRR4302536.232148/1‑94 (MQ=60)
                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAAGGGCTGCTTATAAATACGGGCTCGGCCGGGTATTGCTGGCGgtcagttaggattcc                                                                  >  SRR4302536.5065782/1‑79 (MQ=60)
                              ACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCTTATAAATAAGGGCTGGGACGGGGATTGCTGGCGGTGATTTAGCTTTCTG                                                                 >  SRR4302536.1297703/1‑94 (MQ=60)
                              ACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTG                                                                 <  SRR4302536.2330772/94‑1 (MQ=60)
                               CCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGCCGGGTCTTGCTGGCGATGctttagctttcttc                                                                >  SRR4302536.4447109/1‑80 (MQ=60)
                                CTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATAAGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTACTGCA                                                               >  SRR4302536.5235812/1‑94 (MQ=60)
                                CTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGATTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCA                                                               >  SRR4302536.5595740/1‑94 (MQ=60)
                                 TATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAG                                                              >  SRR4302536.2224590/1‑94 (MQ=60)
                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGT                                                             <  SRR4302536.1565365/94‑1 (MQ=60)
                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302536.2819373/94‑1 (MQ=60)
                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302536.3542260/94‑1 (MQ=60)
                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCCTCCTGCAGTCT                                                           <  SRR4302536.4196074/94‑1 (MQ=60)
                                      CAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCCTTCTGCAGTCTGG                                                         >  SRR4302536.2729866/1‑94 (MQ=60)
                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGGTTCCTGCAGTCTTGC                                                        >  SRR4302536.6430350/1‑94 (MQ=60)
                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCCGTCTGGC                                                        >  SRR4302536.4799660/1‑94 (MQ=60)
                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATAAGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTCGC                                                        >  SRR4302536.4118402/1‑94 (MQ=60)
                                         TGCCAGTTCGTTTATCGGCGGGCCAGGAGGTGGTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGAATCAGCGTTCTGCAGGCTGGCTT                                                      >  SRR4302536.1704635/1‑94 (MQ=53)
                                         TGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTT                                                      <  SRR4302536.4253222/94‑1 (MQ=60)
                                         TGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTAAGCTTCCTGCAGTCTGGCTT                                                      <  SRR4302536.6768186/94‑1 (MQ=60)
                                          GCTAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTT                                                     <  SRR4302536.406256/94‑1 (MQ=60)
                                          GCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTT                                                     <  SRR4302536.1854457/94‑1 (MQ=60)
                                           CCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTTGCGATGATTTAGCTTCCTGCAGgctgggtttt                                                    >  SRR4302536.3745890/1‑84 (MQ=60)
                                           CCAGTGCGTGTATCGGCCGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATTATTCAGCTTCCTGCAGTCTTGCTTTC                                                    <  SRR4302536.2803912/94‑1 (MQ=60)
                                            CAGTTCGTTTATCGGCGGGCCAGGAGGTGCTTTTGAATACGGGCTGGGCCGGGTTTTTGTTGCGATGATTCAGCTTTCTTGAGTCTGGGTTTCA                                                   >  SRR4302536.6593874/1‑94 (MQ=26)
                                            CAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCA                                                   <  SRR4302536.3131331/94‑1 (MQ=60)
                                            CAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCA                                                   <  SRR4302536.6365475/94‑1 (MQ=60)
                                             tgtttttttcggGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAC                                                  <  SRR4302536.4086183/82‑1 (MQ=60)
                                             AGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGGCTGGGTTTCAC                                                  >  SRR4302536.4375977/1‑94 (MQ=60)
                                              GTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACT                                                 <  SRR4302536.2266405/94‑1 (MQ=60)
                                                 CGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGGTTTCACTCGG                                              >  SRR4302536.6893869/1‑94 (MQ=60)
                                                  GTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGT                                             >  SRR4302536.3659982/1‑94 (MQ=60)
                                                  GTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGT                                             <  SRR4302536.1709760/94‑1 (MQ=60)
                                                  GTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTTACTCGGT                                             >  SRR4302536.3780482/1‑94 (MQ=60)
                                                   TTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTTGGCCGGGGGTTGCTGGCGATGATTCCGCTTTCTGCAGGCTGGCTTTCACTCcgtg                                            >  SRR4302536.3728528/1‑90 (MQ=57)
                                                   TTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTA                                            <  SRR4302536.4311881/94‑1 (MQ=60)
                                                   TTTATCGGCGGGCCAGGAGCGGCTTATAAATACGGGCTGGGCCGGGTATTGCTGTCGGTGATTCAGgtttctgccgtcttgcttttcctcggtt                                            >  SRR4302536.1126572/1‑66 (MQ=60)
                                                    TTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAT                                           <  SRR4302536.1894507/94‑1 (MQ=60)
                                                      ATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTT                                         >  SRR4302536.2488005/1‑94 (MQ=60)
                                                       TCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCT                                        <  SRR4302536.203948/94‑1 (MQ=60)
                                                       TCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCT                                        <  SRR4302536.1835105/94‑1 (MQ=60)
                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTC                                       <  SRR4302536.6505935/94‑1 (MQ=60)
                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTC                                       <  SRR4302536.5561515/94‑1 (MQ=60)
                                                         GGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCGCTCGGTATTCTCG                                      <  SRR4302536.5205527/94‑1 (MQ=60)
                                                          GGGGGCGAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGG                                     <  SRR4302536.3227252/94‑1 (MQ=60)
                                                           CGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGC                                    >  SRR4302536.610507/1‑94 (MQ=60)
                                                            GGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCA                                   <  SRR4302536.3927121/94‑1 (MQ=60)
                                                             GGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAA                                  >  SRR4302536.6078390/1‑94 (MQ=60)
                                                             GGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAA                                  >  SRR4302536.3342088/1‑94 (MQ=60)
                                                               CCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGA                                >  SRR4302536.4685172/1‑94 (MQ=60)
                                                               CCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGA                                >  SRR4302536.1827814/1‑94 (MQ=60)
                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGAAAGAA                               >  SRR4302536.2062843/1‑94 (MQ=60)
                                                                 AGGAGTTGCTTATAAATGCGGGCTGGTTCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              <  SRR4302536.5271904/94‑1 (MQ=60)
                                                                  GGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGT                             >  SRR4302536.6540252/1‑94 (MQ=60)
                                                                  GGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGT                             <  SRR4302536.4876187/94‑1 (MQ=60)
                                                                  GGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGT                             <  SRR4302536.2850139/94‑1 (MQ=60)
                                                                   GAGCTGCTTATAAATACGGGCTGGGGCGAGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            <  SRR4302536.2012884/94‑1 (MQ=60)
                                                                   GAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            <  SRR4302536.2971893/94‑1 (MQ=60)
                                                                   GAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            >  SRR4302536.26362/1‑94 (MQ=60)
                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           >  SRR4302536.5157230/1‑94 (MQ=60)
                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           <  SRR4302536.6542645/94‑1 (MQ=60)
                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           >  SRR4302536.5844648/1‑94 (MQ=60)
                                                                     GCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTG                          >  SRR4302536.6342198/1‑94 (MQ=60)
                                                                     GCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTG                          >  SRR4302536.878963/1‑94 (MQ=60)
                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGC                         >  SRR4302536.5841585/1‑94 (MQ=60)
                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCCGCAAGAAGTTTGC                         >  SRR4302536.4934787/1‑94 (MQ=60)
                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        >  SRR4302536.5837682/1‑94 (MQ=60)
                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        <  SRR4302536.2494803/94‑1 (MQ=60)
                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        <  SRR4302536.3004567/94‑1 (MQ=60)
                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        >  SRR4302536.6218791/1‑94 (MQ=60)
                                                                        GCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGA                       >  SRR4302536.6701860/1‑94 (MQ=60)
                                                                         CTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAT                      >  SRR4302536.6640100/1‑94 (MQ=60)
                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302536.5154688/94‑1 (MQ=60)
                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302536.2245403/94‑1 (MQ=60)
                                                                           TATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTC                    <  SRR4302536.4491772/94‑1 (MQ=60)
                                                                             TAAATACGGGCTGGGCCGGGTATTGCTGGCGTTGATTAAGCTTCCTGCAGTCTGGCTTTCACTCGGGATTCTCGGCAAGAAATTTGCtttcctt                  >  SRR4302536.5731508/1‑87 (MQ=60)
                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302536.6546347/94‑1 (MQ=60)
                                                                               AATACGGGCTGGGGGGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGC                <  SRR4302536.3647625/94‑1 (MQ=60)
                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302536.1471139/94‑1 (MQ=60)
                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302536.2173089/94‑1 (MQ=60)
                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               >  SRR4302536.6200728/1‑94 (MQ=60)
                                                                                 TACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGC              <  SRR4302536.1103249/94‑1 (MQ=60)
                                                                                 TACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGC              <  SRR4302536.6132649/94‑1 (MQ=60)
                                                                                  ACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGGAGTTTGCGATTCTTGCGCG             >  SRR4302536.1816562/1‑94 (MQ=60)
                                                                                        TGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTA       >  SRR4302536.5331608/1‑94 (MQ=60)
                                                                                        CGGGCCGGGTACTGCTGGCAATGATTCAGCTACCTGCAGTCTGGCTTTCACCCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTA       <  SRR4302536.1905731/94‑1 (MQ=60)
                                                                                          GGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACA     >  SRR4302536.2261713/1‑94 (MQ=60)
                                                                                           GCAGGTTATTTCTGGCGATAATTCAGCTTCCCGCATTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCAATTCTTGCGCGCCGCTACAA    <  SRR4302536.5820613/94‑1 (MQ=60)
                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   >  SRR4302536.3435252/1‑94 (MQ=60)
                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  SRR4302536.4792161/1‑94 (MQ=60)
                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302536.4461648/94‑1 (MQ=60)
                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302536.2035598/94‑1 (MQ=60)
                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302536.995613/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑