Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,057,859 (T)9→8 intergenic (+97/‑167) yihK → / → yihL putative GTP‑binding factor/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,057,8510T.99.2% 1128.8 / ‑1.4 249intergenic (+89/‑175)yihK/yihLputative GTP‑binding factor/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base . (133/113);  minor base G (2/0);  total (135/114)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4057759‑4057939
                                                                                            |                                                                                        
cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGt                                                                                          <  1:5808218/93‑1 (MQ=255)
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                         >  1:3055804/1‑93 (MQ=255)
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                         <  1:4191305/93‑1 (MQ=255)
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                         <  1:849628/93‑1 (MQ=255)
  gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                        >  2:6043697/1‑93 (MQ=255)
   aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                       >  1:6301957/1‑93 (MQ=255)
    cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                      <  1:1669553/93‑1 (MQ=255)
    cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                      >  2:4758369/1‑93 (MQ=255)
    cGGAAAACGATCGTCCCAGCGCAAACCGAGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                      <  2:3922705/93‑1 (MQ=255)
     ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttt                                                                                     >  2:3159724/1‑93 (MQ=255)
       aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                   >  1:2314626/1‑93 (MQ=255)
       aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                   >  1:956518/1‑93 (MQ=255)
        aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                  >  1:1026470/1‑92 (MQ=255)
           cGATCGTCGCCGCGCCAGCCGCGCACCGAAAGACTATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                               <  2:6135011/93‑5 (MQ=255)
             aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                            >  2:2163223/1‑93 (MQ=255)
                gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGt                                                                         <  2:620096/93‑1 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                 >  2:2004001/1‑93 (MQ=255)
                          cAACCCCGCACCGAAAGACAATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCacac                                                               <  1:1175127/93‑1 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                              >  1:3911326/1‑93 (MQ=255)
                            aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCa                                                             >  1:6069852/1‑93 (MQ=255)
                            aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCa                                                             >  2:5810132/1‑93 (MQ=255)
                            aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCa                                                             >  2:5270319/1‑93 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                            <  2:2353080/93‑1 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGAGCACACCaa                                                            >  2:3638419/1‑93 (MQ=255)
                              cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                           <  1:4630567/93‑1 (MQ=255)
                                cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGc                                                         <  2:4042742/93‑1 (MQ=255)
                                cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGc                                                         >  1:4515458/1‑93 (MQ=255)
                                 gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                        >  2:4768231/1‑93 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                       <  2:4468634/93‑1 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                      <  2:4311542/93‑1 (MQ=255)
                                    ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGt                                                     <  2:6484667/93‑1 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                    <  1:755295/93‑1 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                    <  2:2486522/93‑1 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                   <  2:183439/93‑1 (MQ=255)
                                        aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                 >  1:3440117/1‑93 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  1:5101167/1‑93 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  2:2265907/1‑93 (MQ=255)
                                          gACGATTAATTTCGTTTTTCAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  1:6544941/1‑93 (MQ=255)
                                           aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                              <  2:72546/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                             <  1:3624735/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                             >  2:4378464/1‑93 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                             >  2:1484227/1‑93 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                            <  2:5629883/93‑1 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                            >  2:129781/1‑93 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                           <  1:4049475/93‑1 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                           <  1:1715967/93‑1 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                           <  1:333569/93‑1 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                          >  2:106173/1‑93 (MQ=255)
                                               gtagtttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                          <  1:751858/89‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGGTTTTTTTT‑CCTGTCGTGAGCGCACACCAATGCGAGGATCTCCCTtata                                         >  1:3581639/1‑93 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         >  2:1385313/1‑93 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         >  1:723539/1‑93 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  2:6165279/93‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         >  2:4347118/1‑93 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         >  1:370181/1‑93 (MQ=255)
                                                 aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                        <  1:6175080/93‑1 (MQ=255)
                                                 aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                        >  2:6669037/1‑93 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                       <  2:4109338/93‑1 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                       >  2:2563550/1‑93 (MQ=255)
                                                   tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGt                                      >  1:5887420/1‑93 (MQ=255)
                                                    ttCTTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTc                                     <  1:5654065/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  <  2:2689702/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  >  2:5034462/1‑93 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  <  1:4917680/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  <  1:1810622/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  >  1:1266265/1‑93 (MQ=255)
                                                        ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                 <  1:1350606/93‑1 (MQ=255)
                                                           tttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt                              >  2:2955602/1‑93 (MQ=255)
                                                           tttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt                              <  2:3432077/93‑1 (MQ=255)
                                                            ttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa                             >  1:2710323/1‑93 (MQ=255)
                                                            ttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa                             <  2:3615622/93‑1 (MQ=255)
                                                             tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                            >  1:5994021/1‑93 (MQ=255)
                                                             tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                            <  2:6331566/93‑1 (MQ=255)
                                                              aGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTCATAAGTCTGTGGTTACg                           >  1:1688321/1‑93 (MQ=255)
                                                               gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt                          >  2:595516/1‑93 (MQ=255)
                                                               gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt                          >  1:533035/1‑93 (MQ=255)
                                                               gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt                          <  1:253945/93‑1 (MQ=255)
                                                                tCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                         >  1:4352608/1‑93 (MQ=255)
                                                                tCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                         >  2:6304710/1‑93 (MQ=255)
                                                                 cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa                        >  2:4545068/1‑93 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                       <  2:326239/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                       >  2:4324412/1‑93 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                       >  2:1712702/1‑93 (MQ=255)
                                                                    aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                     <  1:1507555/93‑1 (MQ=255)
                                                                    aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                     <  2:4221839/93‑1 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                    >  2:3679250/1‑93 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGG‑TTTTGTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                    <  2:1569856/93‑1 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                   <  1:1985182/93‑1 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                   <  1:5553974/93‑1 (MQ=255)
                                                                       aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                  <  1:5914858/93‑1 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                 >  1:4870477/1‑93 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                 <  1:2964509/93‑1 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                <  1:6206444/93‑1 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  2:5638301/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  1:2452174/1‑93 (MQ=255)
                                                                          ccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc               <  2:3376978/93‑1 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt              <  1:6678546/93‑1 (MQ=255)
                                                                                aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc         <  2:6277178/93‑1 (MQ=255)
                                                                                aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc         >  1:2605527/1‑93 (MQ=255)
                                                                                    aTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa     <  1:639221/93‑1 (MQ=255)
                                                                                            |                                                                                        
CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4057759‑4057939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057766‑4057952
                                                                                             |                                                                                             
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTG                                                                                               >  SRR4302536.1056877/1‑94 (MQ=60)
  AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCC                                                                                            >  SRR4302536.4106198/1‑94 (MQ=60)
   ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                           <  SRR4302536.327253/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                          <  SRR4302536.2043846/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                          <  SRR4302536.1411667/94‑1 (MQ=60)
       TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       >  SRR4302536.2617218/1‑94 (MQ=60)
       TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       >  SRR4302536.3223975/1‑94 (MQ=60)
              CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302536.1736237/1‑94 (MQ=60)
              CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302536.1004138/1‑94 (MQ=60)
               GCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCA                                                                               >  SRR4302536.6560768/1‑94 (MQ=60)
                 GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACA                                                                             <  SRR4302536.156684/94‑1 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302536.4027595/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302536.6355329/1‑94 (MQ=60)
                   CAACCGCGCAACGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAAC                                                                           >  SRR4302536.1798971/1‑94 (MQ=60)
                   CAACCCCGCACCGAAAGACAATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           <  SRR4302536.1210500/94‑1 (MQ=60)
                    AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCA                                                                          >  SRR4302536.6250921/1‑94 (MQ=60)
                    AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCA                                                                          >  SRR4302536.1355351/1‑94 (MQ=60)
                    AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTATCGTGAGCGCACACCA                                                                          >  SRR4302536.6047496/1‑94 (MQ=60)
                     ACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAA                                                                         <  SRR4302536.537992/94‑1 (MQ=60)
                      CCGCGCACCGAAAGACTATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                        <  SRR4302536.1275962/94‑1 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                        <  SRR4302536.6663603/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302536.4769701/94‑1 (MQ=60)
                        GCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGC                                                                      >  SRR4302536.4650891/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302536.1571507/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     >  SRR4302536.813380/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302536.2489773/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302536.3130541/94‑1 (MQ=60)
                          GCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGA                                                                    >  SRR4302536.5755448/1‑94 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302536.776633/1‑94 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                  <  SRR4302536.2890747/94‑1 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302536.880006/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302536.777460/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302536.717043/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302536.6367864/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302536.6066662/1‑94 (MQ=60)
                               GAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATC                                                               >  SRR4302536.5833492/1‑94 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              >  SRR4302536.399776/1‑94 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              >  SRR4302536.3542567/1‑94 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              >  SRR4302536.6526167/1‑94 (MQ=60)
                                 AAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTC                                                             <  SRR4302536.3913911/94‑1 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                            >  SRR4302536.5253686/1‑94 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                            >  SRR4302536.3531338/1‑94 (MQ=60)
                                  AGACGATTAATTTCGTTTTTCAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                            >  SRR4302536.6739841/1‑94 (MQ=60)
                                    ACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          >  SRR4302536.5657126/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         <  SRR4302536.3733135/94‑1 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         >  SRR4302536.4893540/1‑94 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302536.4169515/94‑1 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302536.1765576/94‑1 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302536.4501755/94‑1 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302536.342423/94‑1 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       >  SRR4302536.6745644/1‑94 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       >  SRR4302536.1284302/1‑94 (MQ=60)
                                       ATTAATTTCGTGTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302536.1998764/94‑1 (MQ=60)
                                        gtagTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      <  SRR4302536.773927/90‑1 (MQ=60)
                                        TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGGTTTTTTTT‑CCTGTCGTGAGCGCACACCAATGCGAGGATCTCCCTTATA                                                      >  SRR4302536.3688738/1‑94 (MQ=60)
                                        TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      >  SRR4302536.744706/1‑94 (MQ=60)
                                        TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      >  SRR4302536.379956/1‑94 (MQ=60)
                                         TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                     >  SRR4302536.4732894/1‑94 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    <  SRR4302536.6068409/94‑1 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    <  SRR4302536.2541550/94‑1 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    >  SRR4302536.5436262/1‑94 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    <  SRR4302536.6359004/94‑1 (MQ=60)
                                           ATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   >  SRR4302536.6062764/1‑94 (MQ=60)
                                             TTCTTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 <  SRR4302536.5822098/94‑1 (MQ=60)
                                             TTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 >  SRR4302536.3563841/1‑94 (MQ=60)
                                               CGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGT                                               >  SRR4302536.1304224/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302536.5065463/94‑1 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              >  SRR4302536.6280354/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              >  SRR4302536.6028369/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302536.1865260/94‑1 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                             >  SRR4302536.3469585/1‑94 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                             <  SRR4302536.1390882/94‑1 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                             >  SRR4302536.904720/1‑94 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            >  SRR4302536.3702948/1‑94 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            <  SRR4302536.5046772/94‑1 (MQ=60)
                                                    TTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTA                                          >  SRR4302536.2791171/1‑94 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                         >  SRR4302536.3596912/1‑94 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                         >  SRR4302536.6172706/1‑94 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                         <  SRR4302536.4841638/94‑1 (MQ=60)
                                                      TAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTCATAAGTCTGTGGTTACG                                        >  SRR4302536.1737227/1‑94 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCGTGTCGTGAGGGCGCACCAATGGGAGTATCTCCCTTATCACTCTTTGGTTATGT                                       >  SRR4302536.4770988/1‑94 (MQ=57)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       >  SRR4302536.546655/1‑94 (MQ=60)
                                                        GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTC                                      >  SRR4302536.4482824/1‑94 (MQ=60)
                                                        GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTC                                      >  SRR4302536.683492/1‑94 (MQ=60)
                                                        GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTC                                      <  SRR4302536.260725/94‑1 (MQ=60)
                                                         TCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCA                                     <  SRR4302536.2543968/94‑1 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    >  SRR4302536.1209848/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    >  SRR4302536.2354855/1‑94 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302536.6600273/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302536.74932/94‑1 (MQ=60)
                                                            TAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  >  SRR4302536.6224509/1‑94 (MQ=60)
                                                            TAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  >  SRR4302536.3725900/1‑94 (MQ=60)
                                                            AAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  <  SRR4302536.1359935/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302536.6188084/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302536.613555/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302536.6126653/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302536.2428551/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302536.6365096/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302536.1551978/94‑1 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                               <  SRR4302536.2044891/94‑1 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                               <  SRR4302536.5719407/94‑1 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              <  SRR4302536.6091037/94‑1 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              >  SRR4302536.5017011/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302536.4158187/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302536.2526242/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             <  SRR4302536.3051827/94‑1 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302536.2617941/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302536.5192469/1‑94 (MQ=60)
                                                                  ACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGC                            <  SRR4302536.6391352/94‑1 (MQ=60)
                                                                  ACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGC                            <  SRR4302536.3778955/94‑1 (MQ=60)
                                                                   CCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                           <  SRR4302536.4600239/94‑1 (MQ=60)
                                                                    CTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          <  SRR4302536.6876962/94‑1 (MQ=60)
                                                                       CCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGT                       <  SRR4302536.5057249/94‑1 (MQ=60)
                                                                       CCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGT                       >  SRR4302536.83245/1‑94 (MQ=60)
                                                                        CAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTC                      >  SRR4302536.2683731/1‑94 (MQ=60)
                                                                        CAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTC                      >  SRR4302536.5596157/1‑94 (MQ=60)
                                                                        CAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTC                      >  SRR4302536.5753684/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     >  SRR4302536.1784282/1‑94 (MQ=60)
                                                                           CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   <  SRR4302536.2693198/94‑1 (MQ=60)
                                                                             ATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                 <  SRR4302536.658088/94‑1 (MQ=60)
                                                                              TGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT                >  SRR4302536.4409877/1‑94 (MQ=60)
                                                                              TGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT                >  SRR4302536.5229161/1‑94 (MQ=60)
                                                                               GGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA               <  SRR4302536.1730310/94‑1 (MQ=60)
                                                                               GGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA               <  SRR4302536.6877517/94‑1 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT              <  SRR4302536.4227378/94‑1 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT              >  SRR4302536.1915337/1‑94 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC             >  SRR4302536.3786700/1‑94 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC             >  SRR4302536.4385305/1‑94 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC             <  SRR4302536.2906895/94‑1 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302536.1983248/94‑1 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            >  SRR4302536.964184/1‑94 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302536.2138795/94‑1 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            >  SRR4302536.6443014/1‑94 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302536.2979543/94‑1 (MQ=60)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG           <  SRR4302536.4488706/94‑1 (MQ=60)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG           >  SRR4302536.5193204/1‑94 (MQ=60)
                                                                                     GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          >  SRR4302536.1514505/1‑94 (MQ=60)
                                                                                      TGTTTTTTGCCTGTCTTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA         <  SRR4302536.4204110/94‑1 (MQ=60)
                                                                                       TTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAA        >  SRR4302536.6485246/1‑94 (MQ=60)
                                                                                          TTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGT     >  SRR4302536.510158/1‑94 (MQ=60)
                                                                                           TTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTC    <  SRR4302536.5931249/94‑1 (MQ=60)
                                                                                             TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  <  SRR4302536.6776002/94‑1 (MQ=60)
                                                                                             TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  <  SRR4302536.3626161/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057766‑4057952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑