Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,106,773 A→G V120V (GTA→GTG)  sbp → periplasmic sulfate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,7730AG100.0% 970.8 / NA 296V120V (GTA→GTG) sbpperiplasmic sulfate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (137/159);  total (137/159)

Only 100 of 303 total aligned reads displayed.

AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGG  >  NC_000913/4106683‑4106862
                                                                                          |                                                                                         
aaTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCg                                                                                         <  2:128088/93‑1 (MQ=255)
 aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGGCGGATAACTCCGCACCGTACACTTCCACTATTGCTTTCCTCGTGCGt                                                                                        >  1:1003076/1‑93 (MQ=255)
 aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGt                                                                                        >  2:21863/1‑93 (MQ=255)
 aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGt                                                                                        <  2:1399478/93‑1 (MQ=255)
 aTTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGt                                                                                        >  2:4143168/1‑93 (MQ=255)
  ttGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTa                                                                                       >  1:4281540/1‑93 (MQ=255)
   tGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTaa                                                                                      <  2:3030958/93‑1 (MQ=255)
     cGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAgg                                                                                    >  2:3656349/1‑93 (MQ=255)
     cGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAgg                                                                                    <  2:2900204/93‑1 (MQ=255)
         aCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGaaa                                                                                <  1:1437521/93‑1 (MQ=255)
          cgcgGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAt                                                                               <  2:6116571/93‑1 (MQ=255)
           gcgggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATc                                                                              <  1:5740445/93‑1 (MQ=255)
            cgggcggATTGATAAAGAGTGGATCAACCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATcc                                                                             <  1:1777007/93‑1 (MQ=255)
            cgggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATcc                                                                             >  1:2571650/1‑93 (MQ=255)
            cggccggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATcc                                                                             <  1:5296075/93‑1 (MQ=255)
             gggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCg                                                                            <  2:1463459/93‑1 (MQ=255)
             gggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCg                                                                            >  1:2341586/1‑93 (MQ=255)
             gggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCg                                                                            >  1:5594895/1‑93 (MQ=255)
             gggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCg                                                                            <  2:704650/93‑1 (MQ=255)
              ggcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGa                                                                           >  2:5214983/1‑93 (MQ=255)
               gcggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGaa                                                                          <  2:5483287/93‑1 (MQ=255)
                 ggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGc                                                                        >  2:2344257/1‑93 (MQ=255)
                 ggATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGc                                                                        >  1:2764126/1‑93 (MQ=255)
                  gATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCa                                                                       <  1:5643008/93‑1 (MQ=255)
                   aTTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAg                                                                      >  1:2231344/1‑93 (MQ=255)
                    ttGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGa                                                                     >  1:1520730/1‑93 (MQ=255)
                      gATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATc                                                                   >  2:6362172/1‑93 (MQ=255)
                       aTAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATcc                                                                  >  2:2225060/1‑93 (MQ=255)
                       aTAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATcc                                                                  <  2:433634/93‑1 (MQ=255)
                        tAAAGAGTGTATCAAACGTCTGCCGGATAACTCCGCACCGTACACTCCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  1:6013564/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 >  2:1244822/1‑93 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  2:1577930/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  2:170420/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 >  2:4406554/1‑93 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  1:5368004/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  2:5005668/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 <  1:2911094/93‑1 (MQ=255)
                        tAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCa                                                                 >  2:1975287/1‑93 (MQ=255)
                          aaGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATg                                                               >  2:464955/1‑93 (MQ=255)
                          aaGAGTGGATCAAACATCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATg                                                               <  2:4195182/93‑1 (MQ=255)
                          aaGAGTGGATAAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGCTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATg                                                               >  1:994714/1‑93 (MQ=255)
                           agagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATgc                                                              >  2:3429753/1‑92 (MQ=255)
                           agagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGa                                                              >  1:4416471/1‑93 (MQ=255)
                           agagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGa                                                              >  2:5575528/1‑93 (MQ=255)
                            gagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAc                                                             <  1:5908335/93‑1 (MQ=255)
                            gagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAc                                                             <  1:6286676/93‑1 (MQ=255)
                            gagTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAc                                                             >  1:4899388/1‑93 (MQ=255)
                              gTGGATCAAACGTCTTCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTg                                                           >  1:6728895/1‑93 (MQ=255)
                              gTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTg                                                           >  1:3476891/1‑93 (MQ=255)
                                  aTCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAAc                                                       <  2:5079035/93‑1 (MQ=255)
                                  aTCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAAc                                                       <  1:4026279/93‑1 (MQ=255)
                                    cAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGa                                                     >  2:3919029/1‑93 (MQ=255)
                                    cAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGa                                                     >  2:2710181/1‑93 (MQ=255)
                                     aaaCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGAt                                                    <  1:1250080/93‑1 (MQ=255)
                                      aaCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATc                                                   <  1:2072733/93‑1 (MQ=255)
                                       aCGTCTGCCGGATAACTCCGCCCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCt                                                  <  2:5426735/93‑1 (MQ=255)
                                       aCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCt                                                  >  2:3067637/1‑93 (MQ=255)
                                         gTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGa                                                >  2:3198172/1‑93 (MQ=255)
                                          tCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAt                                               >  2:4453369/1‑93 (MQ=255)
                                            tGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTa                                             >  1:1867142/1‑93 (MQ=255)
                                             gCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            <  2:3750412/93‑1 (MQ=255)
                                             gCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            >  1:3696925/1‑93 (MQ=255)
                                             gCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTaa                                            <  2:4513802/93‑1 (MQ=255)
                                                  aTACCTCCGCACCGTGCACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCgg                                       <  1:788863/93‑1 (MQ=255)
                                                   tAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCggg                                      >  1:852602/1‑93 (MQ=255)
                                                     aCTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTGAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGgtg                                    >  2:4788317/1‑93 (MQ=255)
                                                      cTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGgtgt                                   >  2:2155231/1‑93 (MQ=255)
                                                       tCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGtt                                  <  2:5831497/93‑1 (MQ=255)
                                                       tCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGtt                                  <  1:461167/93‑1 (MQ=255)
                                                        ccGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGttt                                 <  2:4734604/93‑1 (MQ=255)
                                                        ccGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGttt                                 >  1:4701870/1‑93 (MQ=255)
                                                            cccGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGt                             <  1:1310392/92‑1 (MQ=255)
                                                            aCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGt                             <  1:211889/93‑1 (MQ=255)
                                                            aCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGt                             <  2:3350671/93‑1 (MQ=255)
                                                              cGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGa                           >  2:3696824/1‑93 (MQ=255)
                                                              cGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGa                           <  1:5224243/93‑1 (MQ=255)
                                                               gTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAt                          <  1:4120875/93‑1 (MQ=255)
                                                                tACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATc                         >  2:6587709/1‑93 (MQ=255)
                                                                tACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATc                         <  2:6005816/93‑1 (MQ=255)
                                                                  cacTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCAc                       <  1:2503687/93‑1 (MQ=255)
                                                                     ttCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGcc                    <  1:5040553/93‑1 (MQ=255)
                                                                      tCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCt                   <  1:6191292/93‑1 (MQ=255)
                                                                      tCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCt                   <  2:1825601/93‑1 (MQ=255)
                                                                        caccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTaa                 >  1:5840787/1‑93 (MQ=255)
                                                                        caccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTaa                 <  2:3303449/93‑1 (MQ=255)
                                                                         accaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGTACGATCTGATTAAACCGGGTGTTTCGGTGCTCACGCCTAAt                >  1:3586553/1‑93 (MQ=255)
                                                                          ccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATc               >  1:6476804/1‑93 (MQ=255)
                                                                          ccaTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATc               <  1:949486/93‑1 (MQ=255)
                                                                           caTTGTTTTCCTGGTGCGTAAGGTAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAATCCGGGTGTTTCGGTGATCACGCCTAATcc              <  1:3120539/93‑1 (MQ=255)
                                                                            aTTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGTTCTGATTAAACCGGGTATTTCGGTGATCACGCCTAATCCg             <  1:5440874/93‑1 (MQ=255)
                                                                             ttGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGa            >  2:2499881/1‑93 (MQ=255)
                                                                                ttttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaaa         <  2:773250/93‑1 (MQ=255)
                                                                                ttttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaaa         >  1:319769/1‑93 (MQ=255)
                                                                                ttttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGaaaa         <  1:1656929/93‑1 (MQ=255)
                                                                                 tttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAg        <  1:1068799/93‑1 (MQ=255)
                                                                                  ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       <  2:2595752/93‑1 (MQ=255)
                                                                                  ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       <  1:4957960/93‑1 (MQ=255)
                                                                                  ttCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGc       <  1:3833492/93‑1 (MQ=255)
                                                                                    ccTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGctc     <  1:2452861/93‑1 (MQ=255)
                                                                                       ggTGCGTAAGGGAGATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCtgg  >  1:6603253/1‑93 (MQ=255)
                                                                                          |                                                                                         
AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGG  >  NC_000913/4106683‑4106862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTG  >  NC_000913/4106680‑4106864
                                                                                             |                                                                                           
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTG                                                                                             <  SRR4302536.1211617/94‑1 (MQ=60)
 GCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGC                                                                                            >  SRR4302536.6917609/1‑94 (MQ=60)
 GCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGC                                                                                            >  SRR4302536.4971833/1‑94 (MQ=60)
  CAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCGACCATTGTTTTCCTGGTGCG                                                                                           >  SRR4302536.565335/1‑94 (MQ=60)
  CAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCG                                                                                           >  SRR4302536.5375111/1‑94 (MQ=60)
  CAATTGCGGAACGCGGGCGGATTGATAAAGAGGGGATCAAACGTCTGCCGGATACCTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCG                                                                                           <  SRR4302536.2144897/94‑1 (MQ=60)
   AATTGCGGACCGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGT                                                                                          >  SRR4302536.6738464/1‑94 (MQ=60)
   AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGGCGGATAACTCCGCACCGTACACTTCCACTATTGCTTTCCTCGTGCGT                                                                                          >  SRR4302536.1032675/1‑94 (MQ=60)
   AATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGT                                                                                          >  SRR4302536.28721/1‑94 (MQ=60)
    ATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTA                                                                                         >  SRR4302536.4409318/1‑94 (MQ=60)
     TTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAA                                                                                        <  SRR4302536.5440580/94‑1 (MQ=60)
      TGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAG                                                                                       <  SRR4302536.981521/94‑1 (MQ=60)
         TGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGA                                                                                    >  SRR4302536.2416366/1‑94 (MQ=60)
         GGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGA                                                                                    >  SRR4302536.5845772/1‑94 (MQ=60)
            ACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATACCTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAT                                                                                 <  SRR4302536.6063737/94‑1 (MQ=60)
            ACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAT                                                                                 <  SRR4302536.1480164/94‑1 (MQ=60)
            ACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAT                                                                                 <  SRR4302536.838737/94‑1 (MQ=60)
            ACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAAT                                                                                 <  SRR4302536.6191713/94‑1 (MQ=60)
              GCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCC                                                                               <  SRR4302536.5911215/94‑1 (MQ=60)
              GCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCC                                                                               >  SRR4302536.2648983/1‑94 (MQ=60)
              GCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCC                                                                               <  SRR4302536.516368/94‑1 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAACCGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              <  SRR4302536.1830760/94‑1 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              <  SRR4302536.4666821/94‑1 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              >  SRR4302536.614952/1‑94 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              >  SRR4302536.5761236/1‑94 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              >  SRR4302536.2412614/1‑94 (MQ=60)
               CGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATACG                                                                              >  SRR4302536.1905285/1‑94 (MQ=60)
               CGGCCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCG                                                                              <  SRR4302536.5454004/94‑1 (MQ=60)
                GGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGA                                                                             <  SRR4302536.3695256/94‑1 (MQ=60)
                GGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGA                                                                             <  SRR4302536.2828964/94‑1 (MQ=60)
                   CGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGC                                                                          >  SRR4302536.2160164/1‑94 (MQ=60)
                   CGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGC                                                                          >  SRR4302536.2846337/1‑94 (MQ=60)
                   CGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGC                                                                          <  SRR4302536.5059098/94‑1 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                         >  SRR4302536.2142749/1‑94 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                         <  SRR4302536.717006/94‑1 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                         >  SRR4302536.548026/1‑94 (MQ=60)
                    GGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCA                                                                         <  SRR4302536.6433776/94‑1 (MQ=60)
                     GATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAG                                                                        >  SRR4302536.2298839/1‑94 (MQ=60)
                     GATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAG                                                                        <  SRR4302536.5810660/94‑1 (MQ=60)
                      ATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGA                                                                       >  SRR4302536.1565487/1‑94 (MQ=60)
                      ATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGA                                                                       >  SRR4302536.3911166/1‑94 (MQ=60)
                      ATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGA                                                                       <  SRR4302536.1965918/94‑1 (MQ=60)
                          ATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCA                                                                   >  SRR4302536.5234743/1‑94 (MQ=60)
                          ATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCA                                                                   >  SRR4302536.6199133/1‑94 (MQ=60)
                           TAAAGAGTGTATCAAACGTCTGCCGGATAACTCCGCACCGTACACTCCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCAT                                                                  <  SRR4302536.6192836/94‑1 (MQ=60)
                           TAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCAT                                                                  >  SRR4302536.4150961/1‑94 (MQ=60)
                           TAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCAT                                                                  <  SRR4302536.5528076/94‑1 (MQ=60)
                           TAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCAT                                                                  <  SRR4302536.2997031/94‑1 (MQ=60)
                           TAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCAT                                                                  >  SRR4302536.5452401/1‑94 (MQ=60)
                            AAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATG                                                                 <  SRR4302536.4784878/94‑1 (MQ=60)
                            AAAGAGTGGATAAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGCTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATG                                                                 >  SRR4302536.1024067/1‑94 (MQ=60)
                             AAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGA                                                                >  SRR4302536.4548663/1‑94 (MQ=60)
                              AGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAC                                                               >  SRR4302536.3371994/1‑94 (MQ=60)
                              AGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGAC                                                               >  SRR4302536.5046721/1‑94 (MQ=60)
                               GAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACT                                                              <  SRR4302536.592309/94‑1 (MQ=60)
                               GAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACT                                                              <  SRR4302536.6084307/94‑1 (MQ=60)
                               GAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACT                                                              <  SRR4302536.6474022/94‑1 (MQ=60)
                                AGTGGATCAAACGTCTTCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTG                                                             >  SRR4302536.6928855/1‑94 (MQ=60)
                                AGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTG                                                             >  SRR4302536.5875892/1‑94 (MQ=60)
                                AGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTG                                                             >  SRR4302536.3580527/1‑94 (MQ=60)
                                 GTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGG                                                            <  SRR4302536.4517990/94‑1 (MQ=60)
                                  TGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGA                                                           >  SRR4302536.4998804/1‑94 (MQ=60)
                                  TGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGA                                                           >  SRR4302536.1834960/1‑94 (MQ=60)
                                    GATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAAC                                                         >  SRR4302536.1336707/1‑94 (MQ=60)
                                     ATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACG                                                        <  SRR4302536.4145708/94‑1 (MQ=60)
                                      TCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGA                                                       >  SRR4302536.4103192/1‑94 (MQ=60)
                                       CAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGAT                                                      >  SRR4302536.1190796/1‑94 (MQ=60)
                                        AAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATC                                                     <  SRR4302536.767499/94‑1 (MQ=60)
                                        AAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATC                                                     <  SRR4302536.1287609/94‑1 (MQ=60)
                                         AACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCT                                                    >  SRR4302536.5414739/1‑94 (MQ=60)
                                         AACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCT                                                    <  SRR4302536.2135173/94‑1 (MQ=60)
                                         AACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCT                                                    <  SRR4302536.6443071/94‑1 (MQ=60)
                                         AACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCT                                                    <  SRR4302536.5162359/94‑1 (MQ=60)
                                          ACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTG                                                   >  SRR4302536.245167/1‑94 (MQ=60)
                                          ACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTG                                                   >  SRR4302536.3880219/1‑94 (MQ=60)
                                          ACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTG                                                   <  SRR4302536.2600695/94‑1 (MQ=60)
                                          ACGTCTGCCGGATAACTCCGCACCGTACACTGCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTG                                                   <  SRR4302536.144456/94‑1 (MQ=60)
                                           CGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGA                                                  >  SRR4302536.5090897/1‑94 (MQ=60)
                                           CGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGA                                                  <  SRR4302536.4171615/94‑1 (MQ=60)
                                           CGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGA                                                  >  SRR4302536.459008/1‑94 (MQ=60)
                                            GTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAT                                                 <  SRR4302536.2203925/94‑1 (MQ=60)
                                            GTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGAT                                                 <  SRR4302536.691306/94‑1 (MQ=60)
                                             TCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATT                                                >  SRR4302536.1154150/1‑94 (MQ=60)
                                              CTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTA                                               >  SRR4302536.1923346/1‑94 (MQ=60)
                                               TGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAA                                              >  SRR4302536.3807475/1‑94 (MQ=60)
                                               TGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAA                                              <  SRR4302536.2931873/94‑1 (MQ=60)
                                                 CCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAC                                            >  SRR4302536.2533953/1‑94 (MQ=60)
                                                 CCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAC                                            <  SRR4302536.4913901/94‑1 (MQ=60)
                                                 CCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAAC                                            <  SRR4302536.6492500/94‑1 (MQ=60)
                                                   GGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCG                                          >  SRR4302536.314683/1‑94 (MQ=60)
                                                    GATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGG                                         >  SRR4302536.5997488/1‑94 (MQ=60)
                                                     ATACCTCCGCACCGTGCACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                        <  SRR4302536.811962/94‑1 (MQ=60)
                                                     ATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGGAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                        >  SRR4302536.2630099/1‑94 (MQ=60)
                                                     ATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                        >  SRR4302536.862783/1‑94 (MQ=60)
                                                     ATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGG                                        >  SRR4302536.877668/1‑94 (MQ=60)
                                                      TAACTCCGCACCTTACACTTCCACCATTGCTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGG                                       >  SRR4302536.1015137/1‑94 (MQ=60)
                                                       aacccccccCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                      <  SRR4302536.5424956/85‑1 (MQ=60)
                                                       AACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                      <  SRR4302536.6781040/94‑1 (MQ=60)
                                                       AACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                      >  SRR4302536.6622952/1‑94 (MQ=60)
                                                       AACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                      <  SRR4302536.1323929/94‑1 (MQ=60)
                                                       AACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTG                                      <  SRR4302536.6043807/94‑1 (MQ=60)
                                                        ACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGT                                     <  SRR4302536.4471411/94‑1 (MQ=60)
                                                         CTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTT                                    >  SRR4302536.2535558/1‑94 (MQ=60)
                                                         CTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTT                                    <  SRR4302536.1789170/94‑1 (MQ=60)
                                                          TCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTT                                   >  SRR4302536.4843249/1‑94 (MQ=60)
                                                          TCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTT                                   <  SRR4302536.473214/94‑1 (MQ=60)
                                                          TCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTT                                   >  SRR4302536.152237/1‑94 (MQ=60)
                                                          TCCGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTT                                   <  SRR4302536.4257020/94‑1 (MQ=60)
                                                            CGCACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCG                                 >  SRR4302536.5808728/1‑94 (MQ=60)
                                                            CGCACCGTACACTTCCACCATTGTTTGCCTGGTGCGTAAGGGAAATCCGAAGCAGATCGATGACTGGAACGATCTGATTAAACCGGGTGTTTCG                                 <  SRR4302536.2794624/94‑1 (MQ=60)
                                                              CACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGT                               >  SRR4302536.1500210/1‑94 (MQ=60)
                                                               CCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTG                              <  SRR4302536.1349499/94‑1 (MQ=60)
                                                               CCCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATACGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTG                              <  SRR4302536.6820982/94‑1 (MQ=60)
                                                               ACCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTG                              <  SRR4302536.217499/94‑1 (MQ=60)
                                                                CCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGA                             >  SRR4302536.5551027/1‑94 (MQ=60)
                                                                CCGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGA                             <  SRR4302536.5574390/94‑1 (MQ=60)
                                                                CCGTACACTTCCACCAGTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGA                             <  SRR4302536.4829609/94‑1 (MQ=60)
                                                                 CGTACACTTCCGCCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.5651172/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.1390132/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            >  SRR4302536.3605413/1‑94 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.1044842/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.4055741/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.3268189/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.5769878/94‑1 (MQ=60)
                                                                 CGTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGAT                            <  SRR4302536.5379912/94‑1 (MQ=60)
                                                                  GTACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATC                           <  SRR4302536.4243052/94‑1 (MQ=60)
                                                                   TACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCA                          <  SRR4302536.3362552/94‑1 (MQ=60)
                                                                   TACACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCA                          <  SRR4302536.1077708/94‑1 (MQ=60)
                                                                     CACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACG                        <  SRR4302536.2404485/94‑1 (MQ=60)
                                                                     CACTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACG                        <  SRR4302536.2579178/94‑1 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                      <  SRR4302536.2345466/94‑1 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                      >  SRR4302536.3241959/1‑94 (MQ=60)
                                                                       CTTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCC                      >  SRR4302536.5464455/1‑94 (MQ=60)
                                                                        TTCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCT                     <  SRR4302536.5191626/94‑1 (MQ=60)
                                                                         TCCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTA                    <  SRR4302536.6375718/94‑1 (MQ=60)
                                                                          CCACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAA                   >  SRR4302536.6014820/1‑94 (MQ=60)
                                                                           CACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGTACGATCTGATTAAACCGGGTGTTTCGGTGCTCACGCCTAAT                  >  SRR4302536.3693824/1‑94 (MQ=60)
                                                                           CACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAT                  >  SRR4302536.6375527/1‑94 (MQ=60)
                                                                           CACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAAT                  >  SRR4302536.971962/1‑94 (MQ=60)
                                                                            ACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATC                 >  SRR4302536.6670059/1‑94 (MQ=60)
                                                                            ACCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATC                 >  SRR4302536.1648035/1‑94 (MQ=60)
                                                                             CCATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCC                <  SRR4302536.977550/94‑1 (MQ=60)
                                                                              CATTGTTTTCCTGGTGCGTAAGGTAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAATCCGGGTGTTTCGGTGATCACGCCTAATCCG               <  SRR4302536.3211700/94‑1 (MQ=60)
                                                                               ATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGTTCTGATTAAACCGGGTATTTCGGTGATCACGCCTAATCCGA              <  SRR4302536.5603590/94‑1 (MQ=60)
                                                                               ATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTTATCACGCCTAATCCGA              >  SRR4302536.3647763/1‑94 (MQ=60)
                                                                               ATTGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGA              <  SRR4302536.1829463/94‑1 (MQ=60)
                                                                                 TGTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAA            >  SRR4302536.2052919/1‑94 (MQ=60)
                                                                                  GTTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAA           >  SRR4302536.328247/1‑94 (MQ=60)
                                                                                   TTTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAG          <  SRR4302536.1705057/94‑1 (MQ=60)
                                                                                    TTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGC         >  SRR4302536.2264571/1‑94 (MQ=60)
                                                                                    TTTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGC         <  SRR4302536.1100757/94‑1 (MQ=60)
                                                                                     TTCCTTGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGGTTCGGTGATCACGCCTAATCCGAAAAGCT        <  SRR4302536.1693763/94‑1 (MQ=60)
                                                                                     TTCCTGGTGCGTAAGGGAAGTCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCT        <  SRR4302536.897607/94‑1 (MQ=60)
                                                                                     TTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCT        <  SRR4302536.3947657/94‑1 (MQ=60)
                                                                                     TTCCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCT        <  SRR4302536.5106938/94‑1 (MQ=60)
                                                                                       CCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT      <  SRR4302536.3790873/94‑1 (MQ=60)
                                                                                       CCTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT      <  SRR4302536.2526945/94‑1 (MQ=60)
                                                                                       CCTGGTGCGTAAGGGAAAGCAGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCT      <  SRR4302536.3012761/94‑1 (MQ=60)
                                                                                        CTGGTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTG     <  SRR4302536.4294035/94‑1 (MQ=60)
                                                                                         TGGTGCGTAAGGGAGATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGG    >  SRR4302536.6799637/1‑94 (MQ=60)
                                                                                           GTGCGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTG  <  SRR4302536.4450147/94‑1 (MQ=60)
                                                                                             |                                                                                           
CGCAATTGCGGAACGCGGGCGGATTGATAAAGAGTGGATCAAACGTCTGCCGGATAACTCCGCACCGTACACTTCCACCATTGTTTTCCTGGTACGTAAGGGAAATCCGAAGCAGATCCATGACTGGAACGATCTGATTAAACCGGGTGTTTCGGTGATCACGCCTAATCCGAAAAGCTCTGGTG  >  NC_000913/4106680‑4106864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑