Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,133	A→G	G208G (GGA→GGG)	cdh →	CDP‑diacylglycerol phosphotidylhydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,133	0	A	G	97.3%	448.4 / 0.1	147	G208G (GGA→GGG)	cdh	CDP‑diacylglycerol phosphotidylhydrolase
Reads supporting (aligned to +/- strand): ref base A (0/4); new base G (79/63); total (79/68)
Fisher's exact test for biased strand distribution p-value = 4.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 156 total aligned reads displayed.

TGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTC  >  NC_000913/4108042‑4108222

 ggCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCg                                                                                         >  2:4620130/1‑93 (MQ=255)

  gcgcGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGgcgc                                                                                        <  2:4185990/93‑1 (MQ=255)

   cgcgTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCt                                                                                       <  2:2896750/93‑1 (MQ=255)

    gcgTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGTAACATATGGGGCGCTa                                                                                      >  1:4222573/1‑93 (MQ=255)

    gcgTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTa                                                                                      <  2:1257754/93‑1 (MQ=255)

     cgTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTAc                                                                                     <  2:136218/93‑1 (MQ=255)

      gTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACg                                                                                    >  2:4102765/1‑93 (MQ=255)

       tCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACgg                                                                                   <  2:2394192/93‑1 (MQ=255)

        cGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACggg                                                                                  >  2:2266230/1‑93 (MQ=255)

         gggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGc                                                                                 <  1:2816695/93‑1 (MQ=255)

         gggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGc                                                                                 <  1:4326478/93‑1 (MQ=255)

          ggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCt                                                                                <  2:1449326/93‑1 (MQ=255)

            tAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTgg                                                                              <  1:554699/93‑1 (MQ=255)

             aaCGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGc                                                                             >  2:4412773/1‑93 (MQ=255)

              aCGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCg                                                                            <  1:4455229/93‑1 (MQ=255)

              aCGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCg                                                                            >  2:1719969/1‑93 (MQ=255)

               cGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGa                                                                           <  1:418845/93‑1 (MQ=255)

                ggAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGag                                                                          >  1:3097709/1‑92 (MQ=255)

                ggAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAt                                                                          >  2:4003680/1‑93 (MQ=255)

                ggAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAt                                                                          <  1:145515/93‑1 (MQ=255)

                 gAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATg                                                                         <  1:1031555/93‑1 (MQ=255)

                 gAAAGCAAACTGGTCGATCGGAGCCGTTTAAGGCGGCTGGTAGAAGAGCTGCCTTGGGAGAGGGAACATTTGGG‑CGCAACGGGCTGGCGATgg                                                                        <  2:2546203/93‑1 (MQ=255)

                  aaaGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATgg                                                                        >  1:3176901/1‑93 (MQ=255)

                   aaGCGAACTGGTACAACGCAGCCCATTTTTGAAGCTGGCCGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCTCTACGGGCTGGCGATggg                                                                       >  2:1425050/1‑92 (MQ=255)

                    aGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTa                                                                      >  2:275783/1‑93 (MQ=255)

                     gCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAc                                                                     <  1:1812329/93‑1 (MQ=255)

                     gCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAc                                                                     <  1:3190385/93‑1 (MQ=255)

                     acGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAc                                                                     <  2:3782144/92‑1 (MQ=255)

                      cGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGGACg                                                                    >  2:878697/1‑93 (MQ=255)

                       gAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACgg                                                                   >  1:2074625/1‑93 (MQ=255)

                       gAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACgg                                                                   >  1:3155716/1‑93 (MQ=255)

                        aaCTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGc                                                                  >  1:4702223/1‑93 (MQ=255)

                         aCTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCa                                                                 <  2:4676778/93‑1 (MQ=255)

                           tGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCagg                                                               >  1:552075/1‑92 (MQ=255)

                           tGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCaga                                                               >  2:2166763/1‑93 (MQ=255)

                            ggTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCagag                                                              >  2:4281169/1‑93 (MQ=255)

                               aCAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGa                                                           >  1:4371549/1‑93 (MQ=255)

                               aCAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGGACGGCAGAGGGa                                                           >  1:3900477/1‑93 (MQ=255)

                                cAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAt                                                          <  2:4744061/93‑1 (MQ=255)

                                cAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAt                                                          <  2:4512445/93‑1 (MQ=255)

                                 aaCGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATa                                                         <  2:4188563/93‑1 (MQ=255)

                                  aCGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATaa                                                        >  2:3494414/1‑93 (MQ=255)

                                   cGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAc                                                       <  1:1151807/93‑1 (MQ=255)

                                   cGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAc                                                       >  1:2693660/1‑93 (MQ=255)

                                     cAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTc                                                     >  1:1565314/1‑93 (MQ=255)

                                      aGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCCGAGAGATAACTCa                                                    >  2:2020185/1‑93 (MQ=255)

                                        cccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAtt                                                  >  1:3911597/1‑93 (MQ=255)

                                        cccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAtt                                                  >  2:1562677/1‑93 (MQ=255)

                                         ccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAttt                                                 >  1:782768/1‑93 (MQ=255)

                                         ccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGCGATAACTCAttt                                                 >  1:3566899/1‑93 (MQ=255)

                                          cATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTg                                                <  1:787779/93‑1 (MQ=255)

                                          cATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTg                                                >  2:2261218/1‑93 (MQ=255)

                                           aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGt                                               >  1:3060452/1‑93 (MQ=255)

                                           aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGt                                               >  1:4578445/1‑93 (MQ=255)

                                           aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGGGATAACTCATTTGt                                               >  2:1550326/1‑93 (MQ=255)

                                            tttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTa                                              >  1:3561764/1‑93 (MQ=255)

                                            tttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTa                                              >  1:4079354/1‑93 (MQ=255)

                                            tttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTa                                              >  1:2161071/1‑93 (MQ=255)

                                              tATGATGCTGGCAGAAGAAGTACCTGGGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAtt                                            <  1:1301679/93‑1 (MQ=255)

                                              tATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAtt                                            <  1:26221/93‑1 (MQ=255)

                                                tgatgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGc                                          >  2:2494341/1‑93 (MQ=255)

                                                 gatgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGCGATAACTCATTTGTATTGCt                                         >  2:2288153/1‑93 (MQ=255)

                                                  atgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTg                                        <  2:2967883/93‑1 (MQ=255)

                                                  atgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTg                                        <  1:253312/93‑1 (MQ=255)

                                                     cTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCg                                     <  2:1481292/93‑1 (MQ=255)

                                                      tGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGa                                    >  1:3174230/1‑93 (MQ=255)

                                                         cAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGacac                                 >  2:3118267/1‑93 (MQ=255)

                                                           gaagaaGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACaa                               >  1:515011/1‑93 (MQ=255)

                                                            aagaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAc                              <  1:2987462/93‑1 (MQ=255)

                                                             agaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACg                             >  2:3181784/1‑93 (MQ=255)

                                                              gaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGa                            >  2:4268072/1‑93 (MQ=255)

                                                              gaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGa                            >  1:3498787/1‑93 (MQ=255)

                                                               aagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaa                           >  2:3635581/1‑93 (MQ=255)

                                                                agTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaaa                          >  1:140647/1‑93 (MQ=255)

                                                                agTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaaa                          >  1:153441/1‑93 (MQ=255)

                                                                 gTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAt                         <  2:728757/93‑1 (MQ=255)

                                                                 gTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAt                         >  1:1866616/1‑93 (MQ=255)

                                                                  tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        <  1:1306667/93‑1 (MQ=255)

                                                                  tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        <  1:1390354/93‑1 (MQ=255)

                                                                  tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        >  2:2503832/1‑93 (MQ=255)

                                                                  tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        <  1:4030452/93‑1 (MQ=255)

                                                                   aCCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCt                       <  1:1956773/93‑1 (MQ=255)

                                                                    ccTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTa                      >  2:4053360/1‑93 (MQ=255)

                                                                     cTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAc                     >  1:2019637/1‑93 (MQ=255)

                                                                     cTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAc                     <  1:4659752/93‑1 (MQ=255)

                                                                      tGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACt                    >  2:1671197/1‑93 (MQ=255)

                                                                      tGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACt                    <  1:3262302/93‑1 (MQ=255)

                                                                       gAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTg                   >  2:659002/1‑93 (MQ=255)

                                                                         ggCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGAc                 <  1:1607732/93‑1 (MQ=255)

                                                                         ggCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGAc                 <  1:4691250/93‑1 (MQ=255)

                                                                           cgcgTGAACATATGGGGCGCTACGGGCTGTCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGc               <  1:2424739/93‑1 (MQ=255)

                                                                            gcgGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCt              <  1:1750654/93‑1 (MQ=255)

                                                                              gggAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTa            <  2:3377128/93‑1 (MQ=255)

                                                                               ggAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTaa           >  2:3966702/1‑93 (MQ=255)

                                                                                gAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAAc          >  2:2937019/1‑93 (MQ=255)

                                                                                    atatGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTg      >  1:1687291/1‑93 (MQ=255)

                                                                                      atGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTTATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCt    <  2:3539774/93‑1 (MQ=255)

                                                                                      atGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCt    <  1:1442610/93‑1 (MQ=255)

                                                                                      atGGGACGCTACGGGCTGGCGACGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCt    <  1:1572586/93‑1 (MQ=255)

                                                                                       tGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCtt   <  2:2365058/93‑1 (MQ=255)

TGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTC  >  NC_000913/4108042‑4108222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  >  NC_000913/4108040‑4108226

CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGG                                                                                               >  SRR4302544.2224000/1‑94 (MQ=60)

CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGG                                                                                               >  SRR4302544.3714631/1‑94 (MQ=60)

CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGG                                                                                               >  SRR4302544.1650696/1‑94 (MQ=60)

  TGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCG                                                                                             <  SRR4302544.4362683/94‑1 (MQ=60)

  TGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACG                                                                                             <  SRR4302544.4046355/94‑1 (MQ=60)

     CGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGTAACATATGGGGCGCTA                                                                                          >  SRR4302544.4265430/1‑94 (MQ=60)

      GCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTAC                                                                                         >  SRR4302544.1164960/1‑94 (MQ=60)

        GTCGTGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGG                                                                                       <  SRR4302544.3188646/94‑1 (MQ=60)

        GTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGG                                                                                       <  SRR4302544.3509873/94‑1 (MQ=60)

        GTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGG                                                                                       >  SRR4302544.1997109/1‑94 (MQ=60)

          CGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGC                                                                                     >  SRR4302544.1723143/1‑94 (MQ=60)

           GGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302544.2843887/94‑1 (MQ=60)

           GGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302544.4370537/94‑1 (MQ=60)

             GTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGG                                                                                  <  SRR4302544.4603082/94‑1 (MQ=60)

              TAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGC                                                                                 <  SRR4302544.560126/94‑1 (MQ=60)

                ACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGA                                                                               <  SRR4302544.4500102/94‑1 (MQ=60)

                ACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGA                                                                               <  SRR4302544.2621341/94‑1 (MQ=60)

                 CGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAT                                                                              <  SRR4302544.423132/94‑1 (MQ=60)

                 CGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAG                                                                              >  SRR4302544.3127207/1‑94 (MQ=60)

                  GGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATG                                                                             <  SRR4302544.1395940/94‑1 (MQ=60)

                  GGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATG                                                                             <  SRR4302544.146903/94‑1 (MQ=60)

                   GAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGG                                                                            <  SRR4302544.1041822/94‑1 (MQ=60)

                   GAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGG                                                                            >  SRR4302544.3207129/1‑94 (MQ=60)

                      AGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAC                                                                         <  SRR4302544.2188148/94‑1 (MQ=60)

                       GCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACG                                                                        <  SRR4302544.1830025/94‑1 (MQ=60)

                       GCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACG                                                                        <  SRR4302544.3220783/94‑1 (MQ=60)

                       GCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACG                                                                        <  SRR4302544.3220784/94‑1 (MQ=60)

                        CGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGG                                                                       >  SRR4302544.3185736/1‑94 (MQ=60)

                        CGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGG                                                                       >  SRR4302544.2094561/1‑94 (MQ=60)

                         GAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGC                                                                      >  SRR4302544.4748697/1‑94 (MQ=60)

                          AACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCA                                                                     <  SRR4302544.522691/94‑1 (MQ=60)

                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    >  SRR4302544.3660071/1‑94 (MQ=60)

                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    >  SRR4302544.4284488/1‑94 (MQ=60)

                            CTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGG                                                                   >  SRR4302544.557477/1‑94 (MQ=60)

                             TGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAG                                                                  <  SRR4302544.1830584/94‑1 (MQ=60)

                                TACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGA                                                               >  SRR4302544.4415872/1‑94 (MQ=60)

                                TACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGA                                                               <  SRR4302544.787437/94‑1 (MQ=60)

                                TACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGGACGGCAGAGGGA                                                               >  SRR4302544.3939221/1‑94 (MQ=60)

                                   AACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAA                                                            <  SRR4302544.1597287/94‑1 (MQ=60)

                                    ACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAC                                                           <  SRR4302544.4208707/94‑1 (MQ=60)

                                    ACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAC                                                           >  SRR4302544.2719507/1‑94 (MQ=60)

                                     CGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACT                                                          <  SRR4302544.1163263/94‑1 (MQ=60)

                                     CGCAGCCCATTTATGAAGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGATGGCGACGGTACGGCAGAGGGATAACC                                                          >  SRR4302544.3572411/1‑94 (MQ=60)

                                      GCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTC                                                         >  SRR4302544.1580835/1‑94 (MQ=60)

                                       CAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCA                                                        >  SRR4302544.4729169/1‑94 (MQ=60)

                                         GCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATT                                                      >  SRR4302544.3950455/1‑94 (MQ=60)

                                          CCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTT                                                     >  SRR4302544.790348/1‑94 (MQ=60)

                                          CCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGCGATAACTCATTT                                                     >  SRR4302544.3601095/1‑94 (MQ=60)

                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   >  SRR4302544.3089658/1‑94 (MQ=60)

                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   <  SRR4302544.795397/94‑1 (MQ=60)

                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   >  SRR4302544.4624052/1‑94 (MQ=60)

                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  >  SRR4302544.2181594/1‑94 (MQ=60)

                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  >  SRR4302544.4119942/1‑94 (MQ=60)

                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  >  SRR4302544.3595909/1‑94 (MQ=60)

                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  >  SRR4302544.2076852/1‑94 (MQ=60)

                                                TATGATGCTGGCAGAAGAAGTACCTGGGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTG                                               <  SRR4302544.1314478/94‑1 (MQ=60)

                                                TATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTG                                               <  SRR4302544.26473/94‑1 (MQ=60)

                                                    ATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGG                                           <  SRR4302544.255976/94‑1 (MQ=60)

                                                       CTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGA                                        >  SRR4302544.3204435/1‑94 (MQ=60)

                                                         GGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACA                                      <  SRR4302544.854413/94‑1 (MQ=60)

                                                            AGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAA                                   >  SRR4302544.520102/1‑94 (MQ=60)

                                                             GAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAC                                  >  SRR4302544.1568338/1‑94 (MQ=60)

                                                              AAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACG                                 <  SRR4302544.3016118/94‑1 (MQ=60)

                                                               AGAATGACCTGAGGCGCGGGAACAGATGGGGCGCGACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGA                                <  SRR4302544.554798/94‑1 (MQ=60)

                                                               AGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGA                                >  SRR4302544.3532155/1‑94 (MQ=60)

                                                               AGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGA                                >  SRR4302544.1484234/1‑94 (MQ=60)

                                                                 AAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAA                              >  SRR4302544.141979/1‑94 (MQ=60)

                                                                 AAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAA                              >  SRR4302544.154933/1‑94 (MQ=60)

                                                                  agttgCTGAGGCGCGGGATCATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGATTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             <  SRR4302544.1622101/89‑1 (MQ=60)

                                                                  AGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             >  SRR4302544.1884657/1‑94 (MQ=60)

                                                                  AGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             >  SRR4302544.2796933/1‑94 (MQ=60)

                                                                    TACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCT                           <  SRR4302544.4070599/94‑1 (MQ=60)

                                                                    TACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCT                           <  SRR4302544.1319513/94‑1 (MQ=60)

                                                                    TACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCT                           <  SRR4302544.1404232/94‑1 (MQ=60)

                                                                     ACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTA                          <  SRR4302544.1975753/94‑1 (MQ=60)

                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         >  SRR4302544.2039241/1‑94 (MQ=60)

                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        <  SRR4302544.479172/94‑1 (MQ=60)

                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        <  SRR4302544.4705847/94‑1 (MQ=60)

                                                                        TGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTG                       >  SRR4302544.1427424/1‑94 (MQ=60)

                                                                        TGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTG                       <  SRR4302544.3293457/94‑1 (MQ=60)

                                                                          AGCCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGGACGGCAGAGTGATAACACACTAGTAGTGCTGGAGACACAACGAAAgcgacggac                     >  SRR4302544.3641344/1‑85 (MQ=53)

                                                                           GGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACG                    <  SRR4302544.1623578/94‑1 (MQ=60)

                                                                           GGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACG                    <  SRR4302544.4737594/94‑1 (MQ=60)

                                                                             CGCGTGAACATATGGGGCGCTACGGGCTGTCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCT                  <  SRR4302544.2447816/94‑1 (MQ=60)

                                                                              GCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTT                 <  SRR4302544.1767788/94‑1 (MQ=60)

                                                                                  GAACATATGGGGCGCTACGGGCTGGCGATGGTACGGGAGTGTGATAACTGATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCATAACC             >  SRR4302544.2491290/1‑94 (MQ=60)

                                                                                     CATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTG          <  SRR4302544.2874716/94‑1 (MQ=60)

                                                                                     CATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTG          >  SRR4302544.1703928/1‑94 (MQ=60)

                                                                                       TATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCT        >  SRR4302544.3105621/1‑94 (MQ=60)

                                                                                        ATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTT       <  SRR4302544.1456962/94‑1 (MQ=60)

                                                                                        ATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTT       <  SRR4302544.4404459/94‑1 (MQ=60)

                                                                                        ATGGGACGCTACGGGCTGGCGACGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTT       <  SRR4302544.1588135/94‑1 (MQ=60)

                                                                                          GGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCA     >  SRR4302544.4567941/1‑94 (MQ=60)

                                                                                           GGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAG    >  SRR4302544.4319795/1‑94 (MQ=60)

                                                                                             GCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  >  SRR4302544.4182754/1‑94 (MQ=60)

CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  >  NC_000913/4108040‑4108226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`