Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,355	A→G	H314R (CAC→CGC)	metL →	aspartokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,355	0	A	G	99.6%	779.8 / ‑4.8	242	H314R (CAC→CGC)	metL	aspartokinase II
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (113/128); total (114/128)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

Only 100 of 261 total aligned reads displayed.

AACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCG  >  NC_000913/4128265‑4128444

aaCTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCcgcg                                                                                         >  1:3168673/1‑93 (MQ=255)

aaCTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCcgcg                                                                                         >  2:2804148/1‑93 (MQ=255)

 aCTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCga                                                                                        <  2:4145549/93‑1 (MQ=255)

 aCTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCga                                                                                        <  2:3606796/93‑1 (MQ=255)

  cTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCgat                                                                                       >  1:5782078/1‑93 (MQ=255)

     ggcTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCgatgat                                                                                    <  1:970052/92‑1 (MQ=255)

       cTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGt                                                                                  >  2:5752658/1‑93 (MQ=255)

        tGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTc                                                                                 >  2:5471810/1‑93 (MQ=255)

        tGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTc                                                                                 >  2:1831252/1‑93 (MQ=255)

             cTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGttt                                                                            <  2:155468/93‑1 (MQ=255)

             cTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGttt                                                                            >  1:3341515/1‑93 (MQ=255)

             cTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGttt                                                                            <  2:4687589/93‑1 (MQ=255)

              tACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttg                                                                           >  2:6267208/1‑93 (MQ=255)

              tACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttg                                                                           <  2:3365623/93‑1 (MQ=255)

               acacGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgt                                                                          >  1:4137595/1‑92 (MQ=255)

                cacGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgat                                                                         >  2:647951/1‑93 (MQ=255)

                cacGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgat                                                                         <  2:275362/93‑1 (MQ=255)

                cacGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgat                                                                         <  1:5924678/93‑1 (MQ=255)

                  cGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgattg                                                                       >  2:6570186/1‑93 (MQ=255)

                  cGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgattg                                                                       <  2:2395843/93‑1 (MQ=255)

                  cGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgattg                                                                       >  2:1640575/1‑93 (MQ=255)

                   gCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTttgattga                                                                      <  2:6414884/93‑1 (MQ=255)

                    ccGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAg                                                                     <  1:2467602/93‑1 (MQ=255)

                    ccGGATAAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAg                                                                     <  1:5594769/93‑1 (MQ=255)

                     cGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGt                                                                    <  2:903835/93‑1 (MQ=255)

                     cGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGt                                                                    <  1:4398214/93‑1 (MQ=255)

                      ggATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGtt                                                                   >  1:1620679/1‑93 (MQ=255)

                      ggATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGtt                                                                   <  1:2830902/93‑1 (MQ=255)

                       gATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGttt                                                                  <  2:4480567/93‑1 (MQ=255)

                       gATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGttt                                                                  <  2:2553913/93‑1 (MQ=255)

                        aTCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTc                                                                 <  2:4296525/93‑1 (MQ=255)

                         tCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCa                                                                <  2:5410020/93‑1 (MQ=255)

                         tCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCa                                                                <  1:4447117/93‑1 (MQ=255)

                         tCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCa                                                                <  2:4955235/93‑1 (MQ=255)

                           aaGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAgg                                                              >  1:2672078/1‑93 (MQ=255)

                            aGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAggg                                                             >  1:1271052/1‑92 (MQ=255)

                            aGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGt                                                             <  1:2988580/93‑1 (MQ=255)

                            aGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGt                                                             >  1:2856707/1‑93 (MQ=255)

                             ggTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTg                                                            <  1:2613722/93‑1 (MQ=255)

                             ggTGCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTg                                                            <  2:6048479/93‑1 (MQ=255)

                              gTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGc                                                           >  2:3210181/1‑93 (MQ=255)

                                  cACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTgcgcgc                                                       >  1:504189/1‑93 (MQ=255)

                                  cACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGc                                                       >  2:3096979/1‑93 (MQ=255)

                                   aCGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGcc                                                      <  1:6289592/93‑1 (MQ=255)

                                    cgcgCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCa                                                     >  1:5188237/1‑93 (MQ=255)

                                     gcgcATTGCACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGCTTGAGTTTCAGGGGCCCGCCac                                                    >  1:1833325/1‑92 (MQ=255)

                                     gcgcATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAg                                                    >  2:793182/1‑93 (MQ=255)

                                     gcgcATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAg                                                    >  2:111101/1‑93 (MQ=255)

                                      cgcATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGt                                                   <  2:3008607/93‑1 (MQ=255)

                                      cccATTAAACGCTTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTCCCCGCCAGt                                                   <  1:6661643/91‑1 (MQ=255)

                                       gcATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTc                                                  >  1:4726099/1‑93 (MQ=255)

                                       gcATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAATTTCAGGTGCCCGCCAGTc                                                  <  1:6541311/93‑1 (MQ=255)

                                       ccATTGAGCGCGTGCGGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTc                                                  <  1:428758/92‑1 (MQ=255)

                                        cATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCa                                                 <  1:512493/93‑1 (MQ=255)

                                         aTTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAg                                                >  1:5312508/1‑93 (MQ=255)

                                           tGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGa                                              >  1:159124/1‑93 (MQ=255)

                                            gAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAt                                             <  2:5669912/93‑1 (MQ=255)

                                            gAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAt                                             <  2:4820456/93‑1 (MQ=255)

                                              aCGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAttt                                           <  1:2291067/93‑1 (MQ=255)

                                              aCGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAttt                                           >  1:6227796/1‑93 (MQ=255)

                                              aCGCGTGCTGGCCTCCGGTACTGGTGCGCGTAGTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAttt                                           <  1:6470371/93‑1 (MQ=255)

                                                     cTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTg                                    <  2:2039976/93‑1 (MQ=255)

                                                      tGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTgg                                   >  2:3595423/1‑93 (MQ=255)

                                                      ggcccTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTgg                                   <  1:4594357/90‑1 (MQ=255)

                                                       ggCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGgc                                  >  2:1701611/1‑93 (MQ=255)

                                                        gccgccGGTACTGGTGCGCGTATAGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGgcg                                 <  2:5694941/93‑1 (MQ=255)

                                                        gCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGgcg                                 <  1:3959313/93‑1 (MQ=255)

                                                         ccTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGACAGTCAGGATTTCAAACTGgcgc                                >  1:2260921/1‑93 (MQ=255)

                                                          cTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTTGCGCa                               >  1:746986/1‑93 (MQ=255)

                                                          cTCCGGTACTGGTGCGCGTACTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCa                               >  2:3349629/1‑93 (MQ=255)

                                                              ggTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATaaa                           <  1:1731472/93‑1 (MQ=255)

                                                               gTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAag                          >  1:106037/1‑93 (MQ=255)

                                                                tACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAaga                         <  1:3846951/93‑1 (MQ=255)

                                                                  cTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAgaga                       >  2:1709096/1‑93 (MQ=255)

                                                                   tGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGAt                      <  1:4344130/93‑1 (MQ=255)

                                                                   tGGTGCGCGTATTCTCACCAGCCGCGATGATTTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGAATTTCAAACTGGCGCATAAAGAGAt                      <  1:4025925/93‑1 (MQ=255)

                                                                    ggTGCGCTTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATc                     <  1:6147941/93‑1 (MQ=255)

                                                                    ggTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATc                     <  1:3272075/93‑1 (MQ=255)

                                                                    ggTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTAGCGCATAAAGAGATc                     >  1:4896354/1‑93 (MQ=255)

                                                                    ggTGCGCGTATTATCTCCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGCTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATc                     <  1:4094671/93‑1 (MQ=255)

                                                                      tGCGCGTATTGTCCCCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGa                   <  1:5468803/93‑1 (MQ=255)

                                                                      tGCGCGTATTGCCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGa                   <  1:1252445/93‑1 (MQ=255)

                                                                      ggcgcGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGa                   <  1:2100525/92‑1 (MQ=255)

                                                                       gcgcGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAc                  >  2:6656652/1‑93 (MQ=255)

                                                                       gcgcGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAc                  <  1:3724415/93‑1 (MQ=255)

                                                                        cgcgTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAcc                 <  1:3136188/93‑1 (MQ=255)

                                                                           gTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCaaa              <  2:278984/93‑1 (MQ=255)

                                                                           gTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCaaa              >  1:5954778/1‑93 (MQ=255)

                                                                           gTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCaaa              >  1:1064875/1‑93 (MQ=255)

                                                                            tATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAAt             >  2:1344895/1‑93 (MQ=255)

                                                                              ttGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTGAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATcc           <  1:145847/93‑1 (MQ=255)

                                                                              ttGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATcc           >  2:4047482/1‑93 (MQ=255)

                                                                                gTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTg         >  2:5192963/1‑93 (MQ=255)

                                                                                  ccccAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGaa       <  1:3743895/91‑1 (MQ=255)

                                                                                  cACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCGTAAAGAGATCGACCAAATCCTGaa       >  2:1228342/1‑93 (MQ=255)

                                                                                  cACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGaa       >  2:3888447/1‑93 (MQ=255)

                                                                                    ccAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAAc     >  1:1292752/1‑93 (MQ=255)

                                                                                     cAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAAcg    <  1:1162812/93‑1 (MQ=255)

                                                                                       gCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAACTCCTGAAAcgcg  >  2:1955253/1‑93 (MQ=255)

                                                                                       gCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAAcgcg  <  1:276247/93‑1 (MQ=255)

AACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCG  >  NC_000913/4128265‑4128444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGC  >  NC_000913/4128262‑4128447

TGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCG                                                                                              <  SRR4302536.1316873/94‑1 (MQ=60)

 GCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGC                                                                                             >  SRR4302536.5123212/1‑94 (MQ=60)

  CAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCG                                                                                            >  SRR4302536.3261506/1‑94 (MQ=60)

  CAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCG                                                                                            >  SRR4302536.1726063/1‑94 (MQ=60)

   AACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTTGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGA                                                                                           >  SRR4302536.979044/1‑94 (MQ=60)

   AACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGA                                                                                           >  SRR4302536.3237606/1‑94 (MQ=60)

    ACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGAT                                                                                          <  SRR4302536.3239505/94‑1 (MQ=60)

    ACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGAT                                                                                          >  SRR4302536.5954167/1‑94 (MQ=60)

     CTGCGCTGTAGCTCCACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATG                                                                                         <  SRR4302536.3350338/94‑1 (MQ=60)

       TCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGAT                                                                                       <  SRR4302536.5552122/94‑1 (MQ=60)

        GGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATG                                                                                      <  SRR4302536.998679/94‑1 (MQ=60)

        CGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATG                                                                                      >  SRR4302536.6749048/1‑94 (MQ=60)

        CGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATG                                                                                      >  SRR4302536.1812766/1‑94 (MQ=60)

             TAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGT                                                                                 <  SRR4302536.4559779/94‑1 (MQ=60)

             TAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGT                                                                                 >  SRR4302536.2224168/1‑94 (MQ=60)

             TAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGT                                                                                 >  SRR4302536.5907092/1‑94 (MQ=60)

             TAGCTACACGCCGGATCAAGATTCCACGCGCATTGAACGCGTGCTGGCTTCCGTTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGT                                                                                 <  SRR4302536.666145/94‑1 (MQ=60)

               GCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTT                                                                               >  SRR4302536.3440511/1‑94 (MQ=60)

                CTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTG                                                                              <  SRR4302536.1094658/94‑1 (MQ=60)

                CTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTG                                                                              <  SRR4302536.229272/94‑1 (MQ=60)

                 TACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGT                                                                             >  SRR4302536.4260314/1‑94 (MQ=60)

                 TACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGA                                                                             >  SRR4302536.6422227/1‑94 (MQ=60)

                  ACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGAT                                                                            >  SRR4302536.1330381/1‑94 (MQ=60)

                  ACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGAT                                                                            >  SRR4302536.1170707/1‑94 (MQ=60)

                   CACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATT                                                                           <  SRR4302536.6101165/94‑1 (MQ=60)

                    ACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTG                                                                          >  SRR4302536.4647683/1‑94 (MQ=60)

                    ACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTG                                                                          >  SRR4302536.1639449/1‑94 (MQ=60)

                      GCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAG                                                                        >  SRR4302536.3857294/1‑94 (MQ=60)

                      GCCGGAGCGAGGTGCCGCGCGCATGGAACGCGAGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAG                                                                        <  SRR4302536.2871345/94‑1 (MQ=60)

                       CCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGCTTGAGT                                                                       >  SRR4302536.6478900/1‑94 (MQ=60)

                       CCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGT                                                                       <  SRR4302536.2542101/94‑1 (MQ=60)

                       CCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGT                                                                       <  SRR4302536.3930861/94‑1 (MQ=60)

                       CCGGATAAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGT                                                                       <  SRR4302536.5761106/94‑1 (MQ=60)

                        CGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTT                                                                      >  SRR4302536.6105968/1‑94 (MQ=60)

                        CGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTT                                                                      >  SRR4302536.1667888/1‑94 (MQ=60)

                        CGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTT                                                                      <  SRR4302536.4529641/94‑1 (MQ=60)

                         GGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTT                                                                     <  SRR4302536.1015668/94‑1 (MQ=60)

                         GGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTT                                                                     <  SRR4302536.2914709/94‑1 (MQ=60)

                         GGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTT                                                                     >  SRR4302536.4071322/1‑94 (MQ=60)

                         GGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTT                                                                     <  SRR4302536.2014414/94‑1 (MQ=60)

                         GGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTT                                                                     <  SRR4302536.1186494/94‑1 (MQ=60)

                          GATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTC                                                                    <  SRR4302536.2831510/94‑1 (MQ=60)

                          GATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTCAGATTC                                                                    >  SRR4302536.6251932/1‑94 (MQ=60)

                           ATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCA                                                                   >  SRR4302536.4087386/1‑94 (MQ=60)

                           ATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCA                                                                   >  SRR4302536.6814923/1‑94 (MQ=60)

                           ATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCA                                                                   >  SRR4302536.4431211/1‑94 (MQ=60)

                            caaagttcccacgggctttgacgggtggcgggcctcgggtcctggggcggGTATTGTC‑CCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGG                                                                 <  SRR4302536.2087068/44‑1 (MQ=60)

                            TCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAG                                                                  >  SRR4302536.4790198/1‑94 (MQ=60)

                            TCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAG                                                                  <  SRR4302536.4580659/94‑1 (MQ=60)

                             CAAGGTTCCACGCGCATTGAACGCGTGCTGTCCTCCGGTTCTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGG                                                                 <  SRR4302536.60296/94‑1 (MQ=60)

                             CAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCATCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGG                                                                 >  SRR4302536.1574462/1‑94 (MQ=60)

                             CAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGG                                                                 >  SRR4302536.2702347/1‑94 (MQ=60)

                             CAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGG                                                                 >  SRR4302536.2751973/1‑94 (MQ=60)

                              AAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGT                                                                <  SRR4302536.4365465/94‑1 (MQ=60)

                              AAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGT                                                                >  SRR4302536.2941162/1‑94 (MQ=60)

                              AAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGT                                                                >  SRR4302536.42102/1‑94 (MQ=60)

                              AAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGG                                                                >  SRR4302536.1309131/1‑94 (MQ=60)

                               AGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTG                                                               <  SRR4302536.3076515/94‑1 (MQ=60)

                               AGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTG                                                               <  SRR4302536.1320405/94‑1 (MQ=60)

                               AGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGCTTCCGGTG                                                               >  SRR4302536.1818720/1‑94 (MQ=60)

                                GGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGC                                                              <  SRR4302536.2692152/94‑1 (MQ=60)

                                GGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGC                                                              <  SRR4302536.3420410/94‑1 (MQ=60)

                                  ttgcacaagctttgtACGCGTGCTCGCCTCCGGTACTGGTGCGCGTATTGTCACCAGGCGCGATGATCTGTGGTTGATTGAGTTTCAGGTGttc                                                            >  SRR4302536.917212/16‑91 (MQ=56)

                                    CCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCGCGC                                                          >  SRR4302536.517148/1‑94 (MQ=60)

                                     CACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCC                                                         <  SRR4302536.2478053/94‑1 (MQ=60)

                                      ACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCA                                                        >  SRR4302536.558428/1‑94 (MQ=60)

                                      ACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCA                                                        >  SRR4302536.5342978/1‑94 (MQ=60)

                                      ACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCA                                                        <  SRR4302536.6477011/94‑1 (MQ=60)

                                       CGCGCATTGCACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGCTTGAGTTTCAGGGGCCCGCCAC                                                       >  SRR4302536.1888594/1‑94 (MQ=60)

                                       CGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAG                                                       <  SRR4302536.5704966/94‑1 (MQ=60)

                                        GCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGG                                                      >  SRR4302536.526908/1‑94 (MQ=60)

                                         CGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTC                                                     >  SRR4302536.4868294/1‑94 (MQ=60)

                                         CCCATTAAACGCTTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTCCCCGCCAGTC                                                     <  SRR4302536.6859625/94‑1 (MQ=60)

                                          GCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCA                                                    >  SRR4302536.6909162/1‑94 (MQ=60)

                                          GCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAATTTCAGGTGCCCGCCAGTCA                                                    <  SRR4302536.6736120/94‑1 (MQ=60)

                                          CCATTGAGCGCGTGCGGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCA                                                    <  SRR4302536.440047/94‑1 (MQ=60)

                                           CATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAG                                                   <  SRR4302536.525647/94‑1 (MQ=60)

                                           CATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAG                                                   >  SRR4302536.5471134/1‑94 (MQ=60)

                                             TTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGA                                                 >  SRR4302536.163238/1‑94 (MQ=60)

                                             TTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGCTGATGTCTAGTTGATTGAGCTTCAGGTGCCCGCCAGGCAGGG                                                 >  SRR4302536.5942437/1‑94 (MQ=60)

                                              TGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGAT                                                <  SRR4302536.6682475/94‑1 (MQ=60)

                                                AACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTT                                              >  SRR4302536.6413350/1‑94 (MQ=60)

                                                 ACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             <  SRR4302536.264475/94‑1 (MQ=60)

                                                 ACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             >  SRR4302536.4213313/1‑94 (MQ=60)

                                                 ACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             <  SRR4302536.2360569/94‑1 (MQ=60)

                                                 ACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             <  SRR4302536.2935572/94‑1 (MQ=60)

                                                 ACGCGTGCTGGCCTCCGGTACTGGTGCGCGTAGTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             <  SRR4302536.6663476/94‑1 (MQ=60)

                                                 ACGCGTGCTGCCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTC                                             <  SRR4302536.2385934/94‑1 (MQ=60)

                                                  CGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCA                                            >  SRR4302536.2759816/1‑94 (MQ=60)

                                                      TGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACT                                        <  SRR4302536.1291500/94‑1 (MQ=60)

                                                      TGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACT                                        >  SRR4302536.1212083/1‑94 (MQ=60)

                                                         ggcCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGC                                     <  SRR4302536.4732284/91‑1 (MQ=60)

                                                         TGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGC                                     >  SRR4302536.2349319/1‑94 (MQ=60)

                                                          gccccCCGGTGCTGGTGCGCTTATTGCCACCAGCCGCGAGGATGTCTGTTTGATTGAGTTTCGGGTGCCCGCCAGTCAGGATTTCAAACTGGCG                                    <  SRR4302536.5141855/89‑1 (MQ=60)

                                                           GCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGC                                   <  SRR4302536.4076883/94‑1 (MQ=60)

                                                           GCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGC                                   <  SRR4302536.3836446/94‑1 (MQ=60)

                                                           GCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGACAGTCAGGATTTCAAACTGGCGC                                   >  SRR4302536.2329397/1‑94 (MQ=60)

                                                            CCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTTGCGCA                                  >  SRR4302536.768931/1‑94 (MQ=60)

                                                            CCTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCA                                  >  SRR4302536.5069163/1‑94 (MQ=60)

                                                             CTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCAT                                 <  SRR4302536.2939557/94‑1 (MQ=60)

                                                             CTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCAT                                 <  SRR4302536.5221304/94‑1 (MQ=60)

                                                             CTCCGGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCAT                                 >  SRR4302536.4213071/1‑94 (MQ=60)

                                                               CCGGTCCTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAA                               <  SRR4302536.5033730/94‑1 (MQ=60)

                                                                CGTTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAA                              <  SRR4302536.1305121/94‑1 (MQ=60)

                                                                 GGTCCTGGTGCGCGTTTTGTCACCAGCGGCGATGATGTCTGTTGGATGGAGTTTCAGTTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             <  SRR4302536.6075433/94‑1 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             <  SRR4302536.6423577/94‑1 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             <  SRR4302536.5217609/94‑1 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             <  SRR4302536.3209344/94‑1 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             >  SRR4302536.108867/1‑94 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             >  SRR4302536.4528239/1‑94 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             >  SRR4302536.837635/1‑94 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             <  SRR4302536.1781444/94‑1 (MQ=60)

                                                                 GGTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAG                             >  SRR4302536.2648514/1‑94 (MQ=60)

                                                                  GTACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGA                            <  SRR4302536.58221/94‑1 (MQ=60)

                                                                   TACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAG                           >  SRR4302536.4896559/1‑94 (MQ=60)

                                                                   TACTGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAG                           <  SRR4302536.3961480/94‑1 (MQ=60)

                                                                     CTGTTGCGTGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGAT                         <  SRR4302536.4069729/94‑1 (MQ=60)

                                                                      TGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATC                        <  SRR4302536.4474183/94‑1 (MQ=60)

                                                                      TGGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTAGCGCATAAAGAGATC                        >  SRR4302536.5043612/1‑94 (MQ=60)

                                                                      TGGTGCGCGTATTCTCACCAGCCGCGATGATTTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGAATTTCAAACTGGCGCATAAAGAGATC                        <  SRR4302536.4145345/94‑1 (MQ=60)

                                                                       GGTGCGCTTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       <  SRR4302536.6331101/94‑1 (MQ=60)

                                                                       GGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       <  SRR4302536.5311331/94‑1 (MQ=60)

                                                                       GGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       <  SRR4302536.3368177/94‑1 (MQ=60)

                                                                       GGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       >  SRR4302536.430583/1‑94 (MQ=60)

                                                                       GGTGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       >  SRR4302536.1744624/1‑94 (MQ=60)

                                                                       GGTGCGCGTATTATCTCCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGCTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCG                       <  SRR4302536.4216091/94‑1 (MQ=60)

                                                                         TGCGCGTATTGTCCCCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAC                     <  SRR4302536.5632196/94‑1 (MQ=60)

                                                                         TGCGCGTATTGCCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAC                     <  SRR4302536.1290041/94‑1 (MQ=60)

                                                                         GGCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGAC                     <  SRR4302536.2163692/94‑1 (MQ=60)

                                                                          GCGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACC                    <  SRR4302536.3835738/94‑1 (MQ=60)

                                                                           CGCGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCA                   <  SRR4302536.3227965/94‑1 (MQ=60)

                                                                             CGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAA                 >  SRR4302536.1096717/1‑94 (MQ=60)

                                                                             CGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAA                 >  SRR4302536.6132199/1‑94 (MQ=60)

                                                                             CGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAA                 >  SRR4302536.344081/1‑94 (MQ=60)

                                                                             CGTATTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAA                 <  SRR4302536.3002706/94‑1 (MQ=60)

                                                                              GTATTGTCACCAGCCACGATGGTGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAAT                >  SRR4302536.6645507/1‑94 (MQ=60)

                                                                                 TTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTGAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCT             <  SRR4302536.149652/94‑1 (MQ=60)

                                                                                 TTGTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCT             >  SRR4302536.4432321/1‑94 (MQ=60)

                                                                                   GTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGA           <  SRR4302536.6333382/94‑1 (MQ=60)

                                                                                   GTCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGA           >  SRR4302536.1382955/1‑94 (MQ=60)

                                                                                    TCACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAA          <  SRR4302536.5410076/94‑1 (MQ=60)

                                                                                     CCCCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAA         <  SRR4302536.3855819/94‑1 (MQ=60)

                                                                                     CACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAA         <  SRR4302536.3359005/94‑1 (MQ=60)

                                                                                     CACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAA         >  SRR4302536.3537022/1‑94 (MQ=60)

                                                                                     AACCAGCCGCGATGATGTCTGTTTGATTGAGCTTCAGGCGCCCGCCAGTCAGGATTTCAAACTGGCGCATAACGAGATCGACCAAATACTGAAA         >  SRR4302536.4576290/1‑94 (MQ=60)

                                                                                      ACCAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAAC        >  SRR4302536.1331397/1‑94 (MQ=60)

                                                                                       CCAGCCGCGAGGATGTCTGTTGGATTGAGTTGCAGCTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACG       <  SRR4302536.6239466/94‑1 (MQ=60)

                                                                                        CAGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGC      <  SRR4302536.1197806/94‑1 (MQ=60)

                                                                                         AGCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCG     >  SRR4302536.476114/1‑94 (MQ=60)

                                                                                         AGCCGCGATGATGTCTATTTGATTTGTTTTCAGTTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGTG     <  SRR4302536.1611107/94‑1 (MQ=60)

                                                                                          GCCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGC    <  SRR4302536.283596/94‑1 (MQ=60)

                                                                                          GCCGCGATGATGTCTGTTGGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGC    <  SRR4302536.3043117/94‑1 (MQ=60)

                                                                                           CCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCG   >  SRR4302536.3187802/1‑94 (MQ=60)

                                                                                           CCGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCG   >  SRR4302536.3749410/1‑94 (MQ=60)

                                                                                            CGCGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGC  <  SRR4302536.600895/94‑1 (MQ=60)

TGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGC  >  NC_000913/4128262‑4128447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`