Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,134,509 A→G E274E (GAA→GAG)  b3943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,134,5090AG100.0% 667.6 / NA 204E274E (GAA→GAG) b3943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (93/111);  total (93/111)

Only 100 of 217 total aligned reads displayed.

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598
                                                                                          |                                                                                         
cAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAggg                                                                                         >  1:3525655/1‑93 (MQ=255)
cAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAggg                                                                                         <  2:5797030/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACCCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  1:222904/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        >  1:3644424/1‑93 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  1:495876/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        >  1:175705/1‑93 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  2:1377095/93‑1 (MQ=255)
   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      >  2:841527/1‑93 (MQ=255)
   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      >  1:4794524/1‑93 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     <  2:1109635/93‑1 (MQ=255)
     ttGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCg                                                                                    >  2:944505/1‑93 (MQ=255)
     ttGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCg                                                                                    >  1:4321936/1‑93 (MQ=255)
     ttGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCg                                                                                    >  1:6246796/1‑93 (MQ=255)
       gCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGt                                                                                  >  1:5806319/1‑93 (MQ=255)
       gCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGt                                                                                  >  2:346299/1‑93 (MQ=255)
          aGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTatt                                                                               >  2:4740152/1‑93 (MQ=255)
            cTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgt                                                                             <  2:2444320/93‑1 (MQ=255)
               aaGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgct                                                                          >  1:5962516/1‑93 (MQ=255)
                aGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctg                                                                         >  2:1990832/1‑93 (MQ=255)
                aGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctg                                                                         >  1:522221/1‑93 (MQ=255)
                aGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGAGGGattgtgctg                                                                         >  1:2927047/1‑93 (MQ=255)
                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        >  2:5652197/1‑93 (MQ=255)
                   acgcTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      <  1:3306399/90‑1 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      >  2:5237051/1‑93 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGAGGGattgtggtgatt                                                                      >  1:5512820/1‑93 (MQ=255)
                   acacTGGCCGTTACCGTTTGCGGCGTACTCTTTGCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      <  1:3847213/93‑1 (MQ=255)
                    cacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     <  2:1503198/93‑1 (MQ=255)
                    cacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     >  2:5038506/1‑93 (MQ=255)
                     acTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgt                                                                    <  2:3399275/93‑1 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 >  2:3688325/1‑93 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 <  2:1495630/93‑1 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 >  2:3834611/1‑93 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 >  2:3099927/1‑93 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 <  1:6652093/93‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              <  2:4410358/93‑1 (MQ=255)
                            gTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCa                                                             >  2:4070028/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            <  2:6149206/93‑1 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGGATTGTGCTGATTGTGCTGGCAc                                                            >  1:1189034/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGCGGGCCGTTGAGGGTAGCGGCATTGTGCTGATTGTGCTGGCAc                                                            >  2:1452142/1‑93 (MQ=255)
                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          <  1:4225076/93‑1 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         <  1:5128060/93‑1 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         >  2:624917/1‑93 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         <  1:2711421/93‑1 (MQ=255)
                                   tttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcgc                                                      <  2:3920373/93‑1 (MQ=255)
                                     tGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTg                                                    >  1:1505704/1‑93 (MQ=255)
                                       cggcgTATTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGt                                                  <  2:5519797/93‑1 (MQ=255)
                                       cggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGt                                                  <  2:5077849/93‑1 (MQ=255)
                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 >  1:2041325/1‑93 (MQ=255)
                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 <  1:2742033/93‑1 (MQ=255)
                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 <  1:338240/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:3442355/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:6720784/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:399221/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:1299166/93‑1 (MQ=255)
                                          cgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAg                                               <  1:4060804/93‑1 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  1:1447969/1‑93 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:2153357/1‑93 (MQ=255)
                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           <  1:3002907/93‑1 (MQ=255)
                                               tctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTa                                          >  1:3951136/1‑93 (MQ=255)
                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         <  1:2138669/93‑1 (MQ=255)
                                                 ttttCATGGTGCCTGCTCGGTGAGAATCCGGTGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        <  1:3460947/93‑1 (MQ=255)
                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGa                                       >  2:2019038/1‑93 (MQ=255)
                                                   ttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaa                                      >  2:5929370/1‑93 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   >  2:6528572/1‑93 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   >  1:158987/1‑93 (MQ=255)
                                                        ggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaag                                 >  1:1652861/1‑93 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                <  2:708384/93‑1 (MQ=255)
                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  2:6337302/93‑1 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              <  1:2487649/93‑1 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              >  2:1646969/1‑93 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCAGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              >  1:6542308/1‑93 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  2:5211112/93‑1 (MQ=255)
                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          >  1:3253508/1‑93 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:2515248/93‑1 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        >  1:5425614/1‑93 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:2664660/93‑1 (MQ=255)
                                                                   ggCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgt                      <  1:3191955/93‑1 (MQ=255)
                                                                    gCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgtg                     <  1:3147816/93‑1 (MQ=255)
                                                                         aaTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaa                >  1:3593089/1‑93 (MQ=255)
                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               <  1:6041285/93‑1 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  2:4192603/93‑1 (MQ=255)
                                                                            ccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAgg             <  1:465970/93‑1 (MQ=255)
                                                                            ccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAgg             <  1:6187039/93‑1 (MQ=255)
                                                                            ccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAgg             <  1:170728/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            >  1:6430869/1‑93 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:6699856/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            >  1:4407260/1‑93 (MQ=255)
                                                                              gggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAt           <  2:2091378/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  2:2849215/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          >  1:2951978/1‑93 (MQ=255)
                                                                                gggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCt         <  1:569907/93‑1 (MQ=255)
                                                                                gggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCt         <  1:3939190/93‑1 (MQ=255)
                                                                                gggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCt         <  1:2320415/93‑1 (MQ=255)
                                                                                  gCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGa       <  2:4857769/93‑1 (MQ=255)
                                                                                   ccGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGaa      <  1:2459795/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     >  2:3005027/1‑93 (MQ=255)
                                                                                     gTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCCGTGTAAAAAGGATCTGAAtt    >  1:1114604/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   <  1:125218/93‑1 (MQ=255)
                                                                                       tGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtttt  >  2:1849070/1‑93 (MQ=255)
                                                                                       tGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtttt  >  1:1034414/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTTTC  >  NC_000913/4134416‑4134601
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               >  SRR4302536.156554/1‑94 (MQ=60)
 GCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGG                                                                                              >  SRR4302536.1463430/1‑94 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302536.3715511/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             >  SRR4302536.3630989/1‑94 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302536.1658169/94‑1 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            >  SRR4302536.3753386/1‑94 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            >  SRR4302536.2346785/1‑94 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            >  SRR4302536.180309/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACCCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           <  SRR4302536.228818/94‑1 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302536.2291119/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           <  SRR4302536.508676/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          <  SRR4302536.1446694/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          >  SRR4302536.4938837/1‑94 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         >  SRR4302536.5954306/1‑94 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         >  SRR4302536.4808408/1‑94 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         >  SRR4302536.4433602/1‑94 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        >  SRR4302536.6432876/1‑94 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        >  SRR4302536.4451346/1‑94 (MQ=60)
         TGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      >  SRR4302536.5979241/1‑94 (MQ=60)
         TGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      >  SRR4302536.6397986/1‑94 (MQ=60)
            CAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATT                                                                                   <  SRR4302536.1502624/94‑1 (MQ=60)
             AGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTG                                                                                  <  SRR4302536.6677409/94‑1 (MQ=60)
               CTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTG                                                                                >  SRR4302536.2863832/1‑94 (MQ=60)
               CTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTG                                                                                >  SRR4302536.5354318/1‑94 (MQ=60)
               CTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTG                                                                                >  SRR4302536.4509866/1‑94 (MQ=60)
                TTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGC                                                                               >  SRR4302536.5743704/1‑94 (MQ=60)
                 TAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCT                                                                              >  SRR4302536.6140220/1‑94 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTG                                                                             <  SRR4302536.6329909/94‑1 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTG                                                                             >  SRR4302536.535590/1‑94 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGAGGGATTGTGCTG                                                                             >  SRR4302536.3013433/1‑94 (MQ=60)
                   AGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGA                                                                            >  SRR4302536.803179/1‑94 (MQ=60)
                     CACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATT                                                                          <  SRR4302536.387821/94‑1 (MQ=60)
                     CACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGAGGGATTGTGGTGATT                                                                          >  SRR4302536.5677240/1‑94 (MQ=60)
                      cccctggtcggtccgGTTTGCGGCGTCCTGTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGGGCGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302536.4049801/79‑1 (MQ=60)
                      CCACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCTAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTTCTGATTG                                                                         <  SRR4302536.3614541/94‑1 (MQ=60)
                      ACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302536.3404014/94‑1 (MQ=60)
                      ACACTGGCCGTTACCGTTTGCGGCGTACTCTTTGCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302536.3961749/94‑1 (MQ=60)
                       CACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGT                                                                        <  SRR4302536.5237515/94‑1 (MQ=60)
                        ACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTG                                                                       >  SRR4302536.1163909/1‑94 (MQ=60)
                         CTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGC                                                                      <  SRR4302536.6225623/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302536.6849836/94‑1 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  <  SRR4302536.1674528/94‑1 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  >  SRR4302536.3465813/1‑94 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGGATACGCGGGCCGTTGAGTGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 >  SRR4302536.4712335/1‑94 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 <  SRR4302536.3038774/94‑1 (MQ=60)
                               GTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAC                                                                >  SRR4302536.4952617/1‑94 (MQ=60)
                               GTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGGATTGTGCTGATTGTGCTGGCAC                                                                >  SRR4302536.1224790/1‑94 (MQ=60)
                                TTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCAGATTGTGCTGGCACT                                                               <  SRR4302536.4185473/94‑1 (MQ=60)
                                 TCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCCCTG                                                              >  SRR4302536.2490757/1‑94 (MQ=60)
                                  CCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGG                                                             >  SRR4302536.3950494/1‑94 (MQ=60)
                                  CCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGG                                                             <  SRR4302536.4350793/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302536.5281255/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302536.336021/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302536.3518283/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302536.2792302/94‑1 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           >  SRR4302536.6750666/1‑94 (MQ=60)
                                       TTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTG                                                        >  SRR4302536.1550083/1‑94 (MQ=60)
                                         GCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGT                                                      <  SRR4302536.1860704/94‑1 (MQ=60)
                                          GGGCGGACTCTTTTCGTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     <  SRR4302536.1276780/94‑1 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     >  SRR4302536.2102796/1‑94 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCC‑GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302536.3353947/94‑1 (MQ=60)
                                           GGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302536.2823720/94‑1 (MQ=60)
                                           GGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302536.4076196/94‑1 (MQ=60)
                                           GGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302536.347190/94‑1 (MQ=60)
                                             CGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGC                                                  <  SRR4302536.4181160/94‑1 (MQ=60)
                                             CGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGGAGCGGTATTGTGCTGATTTTGCTGGCACTGGCGCTGGTGAGC                                                  >  SRR4302536.1809054/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 <  SRR4302536.2881878/94‑1 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302536.1490872/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302536.4068484/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              <  SRR4302536.3091217/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302536.2202881/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302536.2807000/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302536.5618108/94‑1 (MQ=60)
                                                    TTTTCATGGTGCCTGCTCGGTGAGAATCCGGTGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGA                                           <  SRR4302536.3564075/94‑1 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       >  SRR4302536.6003926/1‑94 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       >  SRR4302536.163098/1‑94 (MQ=60)
                                                         CTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAA                                      >  SRR4302536.3998428/1‑94 (MQ=60)
                                                         CTGGGGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAA                                      >  SRR4302536.3324136/1‑94 (MQ=60)
                                                          TGGTGGCTGTTCGGCGAGAATCGGGGGGCTGTTGAGGGTAGTGGTATTGTGCTGATTGTGCTGGCACTGGCGATGGTGAGCCGTAAGAAAAAAG                                     <  SRR4302536.3688111/94‑1 (MQ=60)
                                                          TGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAG                                     >  SRR4302536.1700889/1‑94 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302536.5455714/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302536.3074771/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCAGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  >  SRR4302536.6737145/1‑94 (MQ=60)
                                                             GGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302536.1586719/94‑1 (MQ=60)
                                                              GGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGC                                 <  SRR4302536.1704692/94‑1 (MQ=60)
                                                              GGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGC                                 <  SRR4302536.2562696/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302536.6548152/94‑1 (MQ=60)
                                                                CTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCG                               >  SRR4302536.1632922/1‑94 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              >  SRR4302536.3348889/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302536.5587816/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302536.210087/1‑94 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302536.3285541/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCGGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302536.3102458/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302536.3239985/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302536.3240822/94‑1 (MQ=60)
                                                                           GAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAA                   >  SRR4302536.3700561/1‑94 (MQ=60)
                                                                            AATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAA                  >  SRR4302536.4679963/1‑94 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAG                 <  SRR4302536.2556178/94‑1 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAG                 <  SRR4302536.6221466/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGA               <  SRR4302536.175181/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGA               >  SRR4302536.6623076/1‑94 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGA               <  SRR4302536.6371310/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGA               <  SRR4302536.478132/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGA               >  SRR4302536.4539081/1‑94 (MQ=60)
                                                                                CGGGGGCCGTTGAGGTTAGCGGTATTGTGCTGTTTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGAT              <  SRR4302536.446492/94‑1 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATC             <  SRR4302536.5512780/94‑1 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATC             >  SRR4302536.3038976/1‑94 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGAATTGTGCTGATTGTGCTGGGACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGcatt             >  SRR4302536.1567166/1‑90 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCT            <  SRR4302536.1084260/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCT            <  SRR4302536.3701379/94‑1 (MQ=60)
                                                                                  GGGGCCGTGGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCT            <  SRR4302536.605296/94‑1 (MQ=60)
                                                                                   GGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTG           <  SRR4302536.584400/94‑1 (MQ=60)
                                                                                   GGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTG           <  SRR4302536.2390823/94‑1 (MQ=60)
                                                                                   GGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTG           <  SRR4302536.4056227/94‑1 (MQ=60)
                                                                                    GTCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGA          <  SRR4302536.219869/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAAT        <  SRR4302536.2534073/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAAT        <  SRR4302536.6218393/94‑1 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCCGTGT‑AAAAAGGATCTGAATT       >  SRR4302536.1148057/1‑94 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATT       <  SRR4302536.4058438/94‑1 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAAGGATCTGAATT       >  SRR4302536.2619210/1‑94 (MQ=60)
                                                                                         TTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTT     <  SRR4302536.128518/94‑1 (MQ=60)
                                                                                         TTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTT     >  SRR4302536.1065081/1‑94 (MQ=60)
                                                                                          TGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTT    <  SRR4302536.81122/94‑1 (MQ=60)
                                                                                          TGAGGGTAGCGGTATTGTGCTGAGTGTGGTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAACCCGTCAGTGG‑AAAAACGAGCTGAATCTTT    >  SRR4302536.4953043/1‑94 (MQ=60)
                                                                                           GAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTTT   <  SRR4302536.471505/94‑1 (MQ=60)
                                                                                           GAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTTT   >  SRR4302536.802085/1‑94 (MQ=60)
                                                                                            AGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTTTC  >  SRR4302536.1579745/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT‑AAAAAGGATCTGAATTTTTTC  >  NC_000913/4134416‑4134601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑