Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,137,208 A→G T27T (ACT→ACC)  talC ← putative transaldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,137,2080AG97.6% 508.0 / 1.6 167T27T (ACT→ACC) talCputative transaldolase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (77/86);  total (79/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 172 total aligned reads displayed.

AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCT  >  NC_000913/4137118‑4137298
                                                                                          |                                                                                          
aaaCAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGt                                                                                          <  1:126822/93‑1 (MQ=255)
aaaCAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGt                                                                                          <  1:1950513/93‑1 (MQ=255)
 aaCAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGggttggtt                                                                                         <  1:2103357/93‑1 (MQ=255)
   cAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGt                                                                                       <  1:4007979/93‑1 (MQ=255)
   cAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGt                                                                                       <  2:135994/93‑1 (MQ=255)
     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCa                                                                                     >  2:2593314/1‑93 (MQ=255)
     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGGCa                                                                                     >  1:3689084/1‑93 (MQ=255)
       aTGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAcc                                                                                   >  1:2354704/1‑93 (MQ=255)
        tGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccc                                                                                  <  2:187697/93‑1 (MQ=255)
          cccTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccctg                                                                                <  2:2769918/93‑3 (MQ=255)
          cccTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccctg                                                                                <  1:879467/93‑3 (MQ=255)
           ccTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTgg                                                                               >  2:1233532/1‑93 (MQ=255)
            cTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGc                                                                              <  1:1742575/93‑1 (MQ=255)
            cTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGc                                                                              >  1:3783907/1‑93 (MQ=255)
              catcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCaa                                                                            <  2:436630/93‑1 (MQ=255)
              catcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCaa                                                                            >  2:1474235/1‑93 (MQ=255)
               atcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAAt                                                                           >  2:2165229/1‑93 (MQ=255)
               atcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAAt                                                                           <  1:3123978/93‑1 (MQ=255)
               atcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAAt                                                                           >  1:3819643/1‑93 (MQ=255)
                tcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATg                                                                          >  2:3074286/1‑93 (MQ=255)
                  aCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATggg                                                                        <  1:2169731/93‑1 (MQ=255)
                   ccAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATgggg                                                                       <  1:4361656/93‑1 (MQ=255)
                   ccAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATgggg                                                                       >  1:2818258/1‑93 (MQ=255)
                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                      >  2:2422914/1‑93 (MQ=255)
                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                      <  1:3227308/93‑1 (MQ=255)
                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                      >  1:4138014/1‑93 (MQ=255)
                     aaTCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGaa                                                                     <  2:708393/93‑1 (MQ=255)
                      aTCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAat                                                                    <  2:209311/93‑1 (MQ=255)
                       tCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAata                                                                   >  1:4439281/1‑93 (MQ=255)
                       tCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAata                                                                   >  2:2211990/1‑93 (MQ=255)
                       tCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAata                                                                   <  1:11285/93‑1 (MQ=255)
                       tCGCTGTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAata                                                                   <  2:2176266/93‑1 (MQ=255)
                        cGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAatat                                                                  >  1:2726336/1‑93 (MQ=255)
                        cGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAatat                                                                  <  2:3958397/93‑1 (MQ=255)
                        cGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAatat                                                                  <  2:899915/93‑1 (MQ=255)
                        cGCTTTTTGCAGAAGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAatat                                                                  <  1:1583106/93‑1 (MQ=255)
                         gCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATg                                                                 <  2:2524219/93‑1 (MQ=255)
                         gCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATg                                                                 >  2:1802049/1‑93 (MQ=255)
                           tttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCg                                                               >  2:3811493/1‑93 (MQ=255)
                           tttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCg                                                               >  2:1280992/1‑93 (MQ=255)
                           tttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCg                                                               <  1:1569293/93‑1 (MQ=255)
                            ttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGt                                                              <  1:53654/93‑1 (MQ=255)
                            ttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGt                                                              <  1:732410/93‑1 (MQ=255)
                               tGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGcc                                                           <  2:1215840/93‑1 (MQ=255)
                                gCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGccg                                                          >  1:1408929/1‑92 (MQ=255)
                                  aGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGa                                                        <  1:2475258/93‑1 (MQ=255)
                                   gACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAc                                                       <  1:697536/93‑1 (MQ=255)
                                    aCGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACg                                                      <  2:1130995/93‑1 (MQ=255)
                                     cgcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGt                                                     <  1:228448/93‑1 (MQ=255)
                                     cgcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGt                                                     <  1:1836511/93‑1 (MQ=255)
                                      gcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGtt                                                    <  1:2828304/93‑1 (MQ=255)
                                      gcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGtt                                                    <  1:1987028/93‑1 (MQ=255)
                                       cgtcagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTc                                                   <  2:1265575/90‑1 (MQ=255)
                                        ggCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCg                                                  <  2:212258/93‑1 (MQ=255)
                                          cagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGAc                                                <  2:2104825/93‑1 (MQ=255)
                                            gcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACtt                                              <  1:1022526/93‑1 (MQ=255)
                                              aCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCt                                            >  2:4157846/1‑93 (MQ=255)
                                                ttCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGc                                          <  2:919727/93‑1 (MQ=255)
                                                ttCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGc                                          >  2:790694/1‑93 (MQ=255)
                                                ttCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGc                                          <  2:3337864/93‑1 (MQ=255)
                                                   ccATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGAc                                       <  2:1309245/93‑1 (MQ=255)
                                                    cATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACg                                      >  1:1040031/1‑93 (MQ=255)
                                                    cATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACg                                      >  2:2374440/1‑93 (MQ=255)
                                                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTTTGCGACGt                                     <  2:3839589/93‑1 (MQ=255)
                                                      tatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGtt                                    >  1:2016213/1‑93 (MQ=255)
                                                      tatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGtt                                    <  2:4661547/93‑1 (MQ=255)
                                                       atGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGGCGTTa                                   >  2:1709696/1‑93 (MQ=255)
                                                       atGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTa                                   >  1:1367261/1‑93 (MQ=255)
                                                       atGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTa                                   <  2:2932738/93‑1 (MQ=255)
                                                        tGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAg                                  <  2:3365971/93‑1 (MQ=255)
                                                        tGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAg                                  >  1:3324840/1‑93 (MQ=255)
                                                         ggACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                 >  1:881545/1‑93 (MQ=255)
                                                         ggACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                 <  1:1984158/93‑1 (MQ=255)
                                                         ggACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                 <  2:2743588/93‑1 (MQ=255)
                                                                ttGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTcc                          >  2:321783/1‑93 (MQ=255)
                                                                ttGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTcc                          >  2:3132904/1‑93 (MQ=255)
                                                                 tGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCa                         <  1:575689/93‑1 (MQ=255)
                                                                   cTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGa                       >  1:3868998/1‑93 (MQ=255)
                                                                    tGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAt                      >  1:59875/1‑93 (MQ=255)
                                                                    tGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAt                      >  1:3489320/1‑93 (MQ=255)
                                                                    tGGCAGCGATAATGCGCGGGTTGGTTGTCACCCTGGCAATGAGGAATATGCGTGCCAGACGTTCGACTTCTTCGACGTTAGCGGTGTCCAgtt                      >  2:1667136/1‑91 (MQ=255)
                                                                      gCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATAc                    >  2:4406430/1‑93 (MQ=255)
                                                                       cAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACa                   <  1:2553566/93‑1 (MQ=255)
                                                                        aGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAg                  <  2:3710117/93‑1 (MQ=255)
                                                                        aGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAg                  >  1:1876549/1‑93 (MQ=255)
                                                                         gCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGt                 <  2:3466618/93‑1 (MQ=255)
                                                                         gCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGt                 >  1:4675514/1‑93 (MQ=255)
                                                                         gCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGt                 >  1:1800684/1‑93 (MQ=255)
                                                                           gATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTc               <  1:502806/93‑1 (MQ=255)
                                                                             tAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCa             >  2:1619191/1‑93 (MQ=255)
                                                                             tAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGATCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTTCa             >  2:2812083/1‑93 (MQ=255)
                                                                              aaTGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCat            >  2:2137492/1‑93 (MQ=255)
                                                                               aTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatg           <  1:2583035/93‑1 (MQ=255)
                                                                               aTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatg           >  1:1002453/1‑93 (MQ=255)
                                                                               aTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatg           >  1:980730/1‑93 (MQ=255)
                                                                               aTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatg           <  2:4602489/93‑1 (MQ=255)
                                                                                tGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatga          <  2:3517924/93‑1 (MQ=255)
                                                                                   tCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGt       >  2:1187752/1‑93 (MQ=255)
                                                                                       gttggttgTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTcc   <  2:2607346/93‑1 (MQ=255)
                                                                                        ttAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCt  <  2:4021495/93‑1 (MQ=255)
                                                                                          |                                                                                          
AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCT  >  NC_000913/4137118‑4137298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTT  >  NC_000913/4137115‑4137301
                                                                                             |                                                                                             
AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTG                                                                                               >  SRR4302544.2357206/1‑94 (MQ=60)
AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTG                                                                                               <  SRR4302544.2921131/94‑1 (MQ=60)
 GCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGG                                                                                              <  SRR4302544.2596907/94‑1 (MQ=60)
   AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTT                                                                                            <  SRR4302544.127990/94‑1 (MQ=60)
   AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTT                                                                                            <  SRR4302544.1969416/94‑1 (MQ=60)
   AAACAGAATGCCCTCATCACCAATCGCTTTGTGCAGACGCGTCAGCACTTCCCATAGGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTT                                                                                            <  SRR4302544.1085701/94‑1 (MQ=60)
    AACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTG                                                                                           >  SRR4302544.2360836/1‑94 (MQ=60)
    AACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTG                                                                                           <  SRR4302544.2123596/94‑1 (MQ=60)
      CAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTC                                                                                         <  SRR4302544.4047854/94‑1 (MQ=60)
      CAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTC                                                                                         <  SRR4302544.713504/94‑1 (MQ=60)
      CAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTC                                                                                         <  SRR4302544.1645875/94‑1 (MQ=60)
       AGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCA                                                                                        <  SRR4302544.1408946/94‑1 (MQ=60)
       AGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGGCA                                                                                        >  SRR4302544.3725852/1‑94 (MQ=60)
         AATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACC                                                                                      >  SRR4302544.2377012/1‑94 (MQ=60)
          ATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCC                                                                                     >  SRR4302544.443299/1‑94 (MQ=60)
            GCNCTCANCACCAATCGCTTTNTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTG                                                                                   <  SRR4302544.2900627/94‑1 (MQ=60)
            GCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTG                                                                                   <  SRR4302544.528648/94‑1 (MQ=60)
             CCCTCATCCCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGG                                                                                  <  SRR4302544.3929748/94‑1 (MQ=60)
             CCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGG                                                                                  <  SRR4302544.888187/94‑1 (MQ=60)
              CCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGC                                                                                 >  SRR4302544.3821375/1‑94 (MQ=60)
               CTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCA                                                                                <  SRR4302544.1759636/94‑1 (MQ=60)
                TCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAA                                                                               >  SRR4302544.2755994/1‑94 (MQ=60)
                TCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAA                                                                               >  SRR4302544.1034527/1‑94 (MQ=60)
                 CATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAAT                                                                              >  SRR4302544.3857506/1‑94 (MQ=60)
                  ATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATG                                                                             <  SRR4302544.3153672/94‑1 (MQ=60)
                    CACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGG                                                                           >  SRR4302544.3804021/1‑94 (MQ=60)
                    CACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGG                                                                           <  SRR4302544.693164/94‑1 (MQ=60)
                     ACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGG                                                                          >  SRR4302544.2845470/1‑94 (MQ=60)
                     ACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGG                                                                          <  SRR4302544.2190308/94‑1 (MQ=60)
                      CCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGA                                                                         <  SRR4302544.4405915/94‑1 (MQ=60)
                      CCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGA                                                                         >  SRR4302544.4178996/1‑94 (MQ=60)
                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                        <  SRR4302544.3258171/94‑1 (MQ=60)
                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                        >  SRR4302544.3394264/1‑94 (MQ=60)
                         ATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATA                                                                      >  SRR4302544.4484038/1‑94 (MQ=60)
                          TCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATAT                                                                     >  SRR4302544.2752596/1‑94 (MQ=60)
                          TCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATAT                                                                     <  SRR4302544.11386/94‑1 (MQ=60)
                           CGCTTTTTGCAGAAGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATG                                                                    <  SRR4302544.1598746/94‑1 (MQ=60)
                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCCCCCTGGCAATGGGGAATATGCG                                                                  <  SRR4302544.1300424/94‑1 (MQ=60)
                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCG                                                                  >  SRR4302544.241308/1‑94 (MQ=60)
                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCG                                                                  >  SRR4302544.2011413/1‑94 (MQ=60)
                              TTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGT                                                                 <  SRR4302544.1584829/94‑1 (MQ=60)
                               TTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTG                                                                <  SRR4302544.54198/94‑1 (MQ=60)
                               TTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTG                                                                <  SRR4302544.739573/94‑1 (MQ=60)
                                  TGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCG                                                             >  SRR4302544.1422955/1‑94 (MQ=60)
                                   GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAG                                                            >  SRR4302544.2626600/1‑94 (MQ=60)
                                    CAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGA                                                           >  SRR4302544.797630/1‑94 (MQ=60)
                                     AGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAC                                                          <  SRR4302544.925089/94‑1 (MQ=60)
                                     AGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAC                                                          <  SRR4302544.2498927/94‑1 (MQ=60)
                                      GACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACG                                                         <  SRR4302544.704483/94‑1 (MQ=60)
                                      GACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACG                                                         <  SRR4302544.1780880/94‑1 (MQ=60)
                                      GACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACG                                                         <  SRR4302544.1535775/94‑1 (MQ=60)
                                        CGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTT                                                       <  SRR4302544.230818/94‑1 (MQ=60)
                                        CGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTT                                                       <  SRR4302544.1854344/94‑1 (MQ=60)
                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                      <  SRR4302544.2855629/94‑1 (MQ=60)
                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                      <  SRR4302544.2006321/94‑1 (MQ=60)
                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACT                                                  >  SRR4302544.1913231/1‑94 (MQ=60)
                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCACACGTTCGACT                                                  >  SRR4302544.2036001/1‑94 (MQ=60)
                                               GCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTC                                                <  SRR4302544.1032742/94‑1 (MQ=60)
                                                  CTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGC                                             >  SRR4302544.580903/1‑94 (MQ=60)
                                                      CCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACG                                         >  SRR4302544.1050356/1‑94 (MQ=60)
                                                        ATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTT                                       >  SRR4302544.2589781/1‑94 (MQ=60)
                                                        ATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTT                                       >  SRR4302544.2035798/1‑94 (MQ=60)
                                                         TATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTA                                      >  SRR4302544.1380853/1‑94 (MQ=60)
                                                          ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAG                                     >  SRR4302544.3356510/1‑94 (MQ=60)
                                                          ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAG                                     <  SRR4302544.2220994/94‑1 (MQ=60)
                                                           TGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGC                                    >  SRR4302544.890281/1‑94 (MQ=60)
                                                            GGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCG                                   <  SRR4302544.2003424/94‑1 (MQ=60)
                                                               CTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTG                                >  SRR4302544.4652788/1‑94 (MQ=60)
                                                                  CTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCC                             >  SRR4302544.4356894/1‑94 (MQ=60)
                                                                    TGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAG                           <  SRR4302544.581315/94‑1 (MQ=60)
                                                                     GCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGA                          >  SRR4302544.3907418/1‑94 (MQ=60)
                                                                     GCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGA                          >  SRR4302544.3907419/1‑94 (MQ=60)
                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                         >  SRR4302544.3522582/1‑94 (MQ=60)
                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                         >  SRR4302544.60484/1‑94 (MQ=60)
                                                                       TGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATA                        <  SRR4302544.1991313/94‑1 (MQ=60)
                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAG                     <  SRR4302544.2578082/94‑1 (MQ=60)
                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAG                     >  SRR4302544.1894693/1‑94 (MQ=60)
                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAG                     <  SRR4302544.3573313/94‑1 (MQ=60)
                                                                           AGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGT                    >  SRR4302544.4721724/1‑94 (MQ=60)
                                                                           AGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGT                    >  SRR4302544.1818281/1‑94 (MQ=60)
                                                                              GATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCC                 <  SRR4302544.507753/94‑1 (MQ=60)
                                                                               ATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCA                <  SRR4302544.1863548/94‑1 (MQ=60)
                                                                                 AATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATG              <  SRR4302544.32061/94‑1 (MQ=60)
                                                                                 AATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATG              >  SRR4302544.1012404/1‑94 (MQ=60)
                                                                                 AATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATG              >  SRR4302544.990342/1‑94 (MQ=60)
                                                                                  ATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGA             <  SRR4302544.2607862/94‑1 (MQ=60)
                                                                                           TTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTT    >  SRR4302544.1945533/1‑94 (MQ=60)
                                                                                             GGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTT  >  SRR4302544.870798/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTT  >  NC_000913/4137115‑4137301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑