Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,140,312 G→A M68I (ATG→ATA)  frwC → PTS system fructose‑like enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,3120GA100.0% 1004.8 / NA 267M68I (ATG→ATA) frwCPTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (136/131);  total (136/131)

Only 100 of 271 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401
                                                                                          |                                                                                         
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                        >  2:6055162/1‑93 (MQ=255)
  tgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGc                                                                                       >  2:1810247/1‑93 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  2:1602246/93‑1 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      >  1:5567936/1‑93 (MQ=255)
     aTGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCtt                                                                                    <  2:6729898/93‑1 (MQ=255)
       ggCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTc                                                                                  <  1:1158093/93‑1 (MQ=255)
        gCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTcc                                                                                 >  2:2880192/1‑93 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  1:6539493/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  1:330428/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  1:3255230/93‑1 (MQ=255)
         cAAAGACGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  1:4470388/93‑1 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                               >  1:742336/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  2:2030903/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  1:1825385/1‑93 (MQ=255)
                gcAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCg                                                                         >  2:5983623/1‑93 (MQ=255)
                 cAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                        >  2:5733815/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCCGCGTACa                                                                     >  2:2202017/1‑93 (MQ=255)
                     gCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAt                                                                    <  1:4895146/93‑1 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                   >  2:6674179/1‑93 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                   <  1:4752197/93‑1 (MQ=255)
                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  >  1:3810784/1‑93 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 >  1:1990989/1‑93 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 <  2:354731/93‑1 (MQ=255)
                        cgaggCCGTAGCCGATCCAAATCTGAAAAATCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 <  1:2905291/89‑1 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                <  1:3369551/93‑1 (MQ=255)
                          aTGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGtt                                                               <  1:2065190/93‑1 (MQ=255)
                              cGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTc                                                           <  2:5646235/93‑1 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  1:1508709/1‑93 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  2:6280947/1‑93 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  1:3749010/1‑93 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                         >  1:4430132/1‑93 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:3908927/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:2340779/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  2:3483289/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:1085568/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  2:2329942/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:3017156/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  1:6439928/93‑1 (MQ=255)
                                   ccGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTg                                                      >  2:2660515/1‑93 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     <  2:4026920/93‑1 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCa                                                    >  2:3965400/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:541952/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:520151/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:536927/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  2:6150492/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:6305929/93‑1 (MQ=255)
                                        ccAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCagag                                                 <  2:1857989/93‑1 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGc                                                <  1:1215105/93‑1 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGc                                                <  1:4164477/93‑1 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATAGGTTATTCCATTGCAGAGc                                                >  2:4436787/1‑93 (MQ=255)
                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              >  1:4141372/1‑93 (MQ=255)
                                             tCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTc                                            <  1:2624086/93‑1 (MQ=255)
                                             tCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTc                                            <  2:6425081/93‑1 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           <  2:4729785/93‑1 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           <  1:5119173/93‑1 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          <  2:2792919/93‑1 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  2:4530839/1‑93 (MQ=255)
                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGAGTTCTgc                                         >  2:2106524/1‑93 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   <  1:818748/93‑1 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   <  1:1634887/93‑1 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   <  2:3161296/93‑1 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   <  2:6488737/93‑1 (MQ=255)
                                                       cTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCt                                  >  2:6242656/1‑93 (MQ=255)
                                                       cTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCt                                  <  2:5555470/93‑1 (MQ=255)
                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 >  1:3191286/1‑93 (MQ=255)
                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                <  1:6211682/93‑1 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               <  2:5820877/93‑1 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               >  1:2169027/1‑93 (MQ=255)
                                                           ttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCgg                              >  2:2984124/1‑92 (MQ=255)
                                                            tGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTg                             >  1:429049/1‑93 (MQ=255)
                                                             gATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgc                            >  2:3341818/1‑93 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          >  1:6621533/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:6374736/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  1:61000/1‑93 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      >  2:1285209/1‑93 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      <  1:3630226/93‑1 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:4580420/1‑93 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  1:2546795/1‑93 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:6537978/1‑93 (MQ=255)
                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                <  1:6239597/93‑1 (MQ=255)
                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                <  2:1492199/93‑1 (MQ=255)
                                                                          cGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt               <  1:5298836/93‑1 (MQ=255)
                                                                           gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              >  2:4641391/1‑93 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             >  1:6000808/1‑93 (MQ=255)
                                                                             gCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTggg            >  2:3075006/1‑93 (MQ=255)
                                                                             gCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTggg            >  1:1994378/1‑93 (MQ=255)
                                                                              cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           <  2:1025798/93‑1 (MQ=255)
                                                                              cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           <  2:4354344/93‑1 (MQ=255)
                                                                              cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           <  2:659815/93‑1 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          >  2:4732510/1‑93 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          >  2:5023926/1‑93 (MQ=255)
                                                                                tGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTg         <  2:1553910/93‑1 (MQ=255)
                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATTGGTGCCTGGGTTgg        <  1:4064305/93‑1 (MQ=255)
                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTgg        <  2:801232/93‑1 (MQ=255)
                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGCTgg        >  1:5514894/1‑93 (MQ=255)
                                                                                   cGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTa      <  2:5257669/93‑1 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     <  2:4868087/93‑1 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGACTGGGTTGGTAAc    >  2:3799464/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   >  1:4556208/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   >  2:6229656/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  >  NC_000913/4140219‑4140405
                                                                                             |                                                                                             
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATA                                                                                               <  SRR4302536.4218186/94‑1 (MQ=60)
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATA                                                                                               >  SRR4302536.4076379/1‑94 (MQ=60)
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATA                                                                                               <  SRR4302536.3830532/94‑1 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              <  SRR4302536.1031833/94‑1 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              <  SRR4302536.356489/94‑1 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              >  SRR4302536.1353787/1‑94 (MQ=60)
  TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302536.1670375/94‑1 (MQ=60)
  TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302536.1467906/94‑1 (MQ=60)
  TGTTGTATGACAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             >  SRR4302536.6750863/1‑94 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTTACGCTGATAGTG                                                                                            >  SRR4302536.6736523/1‑94 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                            <  SRR4302536.4236119/94‑1 (MQ=60)
     TGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCC                                                                                          >  SRR4302536.5733707/1‑94 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                        <  SRR4302536.1421947/94‑1 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                        <  SRR4302536.4642376/94‑1 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                        <  SRR4302536.3420835/94‑1 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                        <  SRR4302536.6876716/94‑1 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                        >  SRR4302536.1369913/1‑94 (MQ=60)
        ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                       >  SRR4302536.3909437/1‑94 (MQ=60)
          GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                     <  SRR4302536.1192951/94‑1 (MQ=60)
           GCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCT                                                                                    <  SRR4302536.2582158/94‑1 (MQ=60)
           GCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCT                                                                                    <  SRR4302536.5336244/94‑1 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302536.6734259/94‑1 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302536.3350663/94‑1 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   >  SRR4302536.764124/1‑94 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302536.339206/94‑1 (MQ=60)
            CAAAGCCGCAGTGCCGGATGCCGTAGCCGATGCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302536.2222033/94‑1 (MQ=60)
            CAAAGACGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302536.4604681/94‑1 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                  >  SRR4302536.5332241/1‑94 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                  >  SRR4302536.438085/1‑94 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                  >  SRR4302536.3825009/1‑94 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                  >  SRR4302536.6578820/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                                >  SRR4302536.1880439/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                                <  SRR4302536.4928624/94‑1 (MQ=60)
                   GCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGT                                                                            >  SRR4302536.5715378/1‑94 (MQ=60)
                     AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                          <  SRR4302536.6589572/94‑1 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                         >  SRR4302536.2045939/1‑94 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                         >  SRR4302536.1161381/1‑94 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                         <  SRR4302536.5490343/94‑1 (MQ=60)
                       TGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAT                                                                        <  SRR4302536.3665783/94‑1 (MQ=60)
                        GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                       <  SRR4302536.5042369/94‑1 (MQ=60)
                        GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                       <  SRR4302536.3291162/94‑1 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                      >  SRR4302536.2404940/1‑94 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                      >  SRR4302536.3924368/1‑94 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                      >  SRR4302536.5939719/1‑94 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                      <  SRR4302536.4895223/94‑1 (MQ=60)
                          CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                     >  SRR4302536.2050854/1‑94 (MQ=60)
                          CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                     >  SRR4302536.2718124/1‑94 (MQ=60)
                           cgagGCCGTAGCCGATCCAAATCTGAAAAATCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                    <  SRR4302536.2991034/90‑1 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGAAGGGTACATCGGT                                                                    >  SRR4302536.2321159/1‑94 (MQ=60)
                            GATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                   <  SRR4302536.3469645/94‑1 (MQ=60)
                             ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                  <  SRR4302536.2127392/94‑1 (MQ=60)
                              TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGAGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                 >  SRR4302536.4591859/1‑94 (MQ=60)
                                CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                               >  SRR4302536.5385760/1‑94 (MQ=60)
                                CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                               <  SRR4302536.427275/94‑1 (MQ=60)
                                CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                               <  SRR4302536.3036929/94‑1 (MQ=60)
                                 CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                              <  SRR4302536.2680454/94‑1 (MQ=60)
                                 CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                              >  SRR4302536.1553168/1‑94 (MQ=60)
                                 CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                              >  SRR4302536.3861068/1‑94 (MQ=60)
                                  GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                             <  SRR4302536.4544854/94‑1 (MQ=60)
                                  GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                             <  SRR4302536.2051095/94‑1 (MQ=60)
                                  GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                             >  SRR4302536.4562924/1‑94 (MQ=60)
                                  GTAGCCGATCAAAATCTGAAACACCTGTTTGATATCGGCGTTGCGGGCTTGCCGCTGATAGTGCCTTTCGTCGCAGCGTACATCGGTTATTCCA                                                             <  SRR4302536.5883826/94‑1 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            >  SRR4302536.2095858/1‑94 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            >  SRR4302536.6409000/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           >  SRR4302536.1118097/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302536.4025106/94‑1 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302536.3958400/94‑1 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           >  SRR4302536.3105820/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302536.2411790/94‑1 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          >  SRR4302536.5419121/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302536.4302312/94‑1 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302536.6632358/94‑1 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302536.2093446/94‑1 (MQ=60)
                                      CCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGC                                                         >  SRR4302536.5916597/1‑94 (MQ=60)
                                       CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                        >  SRR4302536.50277/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302536.4059692/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302536.5585211/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302536.533483/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302536.555772/1‑94 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                      >  SRR4302536.474978/1‑94 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                      >  SRR4302536.295698/1‑94 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                      >  SRR4302536.3160998/1‑94 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                     <  SRR4302536.550638/94‑1 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                     <  SRR4302536.6493745/94‑1 (MQ=60)
                                           CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                    >  SRR4302536.1224353/1‑94 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   <  SRR4302536.1203699/94‑1 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   <  SRR4302536.1251600/94‑1 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   <  SRR4302536.4288201/94‑1 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   <  SRR4302536.4049455/94‑1 (MQ=60)
                                             AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302536.4264214/1‑94 (MQ=60)
                                               ATCTGAAAAAACTGTTTGATATCGACGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                                <  SRR4302536.6933375/94‑1 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302536.6437591/94‑1 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302536.1622516/94‑1 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302536.2702806/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              <  SRR4302536.837771/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              <  SRR4302536.5272139/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              >  SRR4302536.1692861/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              >  SRR4302536.6186894/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTCCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGCACATCCGTTCTTCCACTGCCGAGCGCTCTG                                              >  SRR4302536.1334536/1‑94 (MQ=60)
                                                  TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                             <  SRR4302536.3282062/94‑1 (MQ=60)
                                                      AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                         >  SRR4302536.4638042/1‑94 (MQ=60)
                                                       AANCTGTNNNNTATCGGCGTTGCNNNNTNNACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCNGCGCTGG                                        <  SRR4302536.1458739/94‑1 (MQ=60)
                                                        AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGATGGC                                       >  SRR4302536.5675399/1‑94 (MQ=60)
                                                         ACTGTTTGATATCGGCGTTGCGGTCTTGACGCTGATAGTGCCTTTCCCAGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTTTGCGCGGGCT                                      <  SRR4302536.4424785/94‑1 (MQ=60)
                                                         ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                      <  SRR4302536.1682495/94‑1 (MQ=60)
                                                         ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                      <  SRR4302536.842842/94‑1 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCTGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     <  SRR4302536.633417/94‑1 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302536.553606/1‑94 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302536.6670990/1‑94 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302536.3284852/1‑94 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                   >  SRR4302536.2234322/1‑94 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                   <  SRR4302536.6396749/94‑1 (MQ=60)
                                                              TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                 <  SRR4302536.2133638/94‑1 (MQ=60)
                                                              TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                 >  SRR4302536.440343/1‑94 (MQ=60)
                                                                GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCG                               >  SRR4302536.357063/1‑94 (MQ=60)
                                                                 ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                              >  SRR4302536.6818410/1‑94 (MQ=60)
                                                                 ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                              >  SRR4302536.3388643/1‑94 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                             >  SRR4302536.3848227/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302536.5579593/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302536.3386301/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302536.62679/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302536.6369731/1‑94 (MQ=60)
                                                                     CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          <  SRR4302536.5105807/94‑1 (MQ=60)
                                                                     CGGCGATGCGGGCTTGACGCTGATAGTGGCATTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          >  SRR4302536.5602259/1‑94 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         <  SRR4302536.3738803/94‑1 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         >  SRR4302536.788789/1‑94 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         <  SRR4302536.6471622/94‑1 (MQ=60)
                                                                        CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                       >  SRR4302536.2623503/1‑94 (MQ=60)
                                                                          TTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGC                     <  SRR4302536.1793453/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                   <  SRR4302536.6425475/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                   <  SRR4302536.6217125/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                   <  SRR4302536.4879068/94‑1 (MQ=60)
                                                                             CGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                  <  SRR4302536.5456859/94‑1 (MQ=60)
                                                                              GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                 >  SRR4302536.6179699/1‑94 (MQ=60)
                                                                               GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG                >  SRR4302536.2671580/1‑94 (MQ=60)
                                                                               GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG                >  SRR4302536.2054379/1‑94 (MQ=60)
                                                                               GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAAATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG                >  SRR4302536.6289111/1‑94 (MQ=60)
                                                                                 CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT              >  SRR4302536.3337093/1‑94 (MQ=60)
                                                                                 CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT              <  SRR4302536.3992506/94‑1 (MQ=60)
                                                                                   TGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGG            <  SRR4302536.1944606/94‑1 (MQ=60)
                                                                                   TGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGCTGG            >  SRR4302536.5679370/1‑94 (MQ=60)
                                                                                    GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATTGGTGCCTGGGTTGGT           <  SRR4302536.4184766/94‑1 (MQ=60)
                                                                                    GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATTGGTGCCTGGGTTGGT           <  SRR4302536.4184767/94‑1 (MQ=60)
                                                                                        CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA       >  SRR4302536.4692869/1‑94 (MQ=60)
                                                                                        CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA       <  SRR4302536.4539989/94‑1 (MQ=60)
                                                                                          GATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC     >  SRR4302536.3553843/1‑94 (MQ=60)
                                                                                          GATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC     >  SRR4302536.2637238/1‑94 (MQ=60)
                                                                                           ATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCT    >  SRR4302536.1176873/1‑94 (MQ=60)
                                                                                             AGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  <  SRR4302536.3015824/94‑1 (MQ=60)
                                                                                             |                                                                                             
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  >  NC_000913/4140219‑4140405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑