Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,082,961 A→G S324P (TCC→CCC)  speA ← biosynthetic arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,9610AG99.4% 1047.2 / ‑3.7 333S324P (TCC→CCC) speAbiosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (187/144);  total (187/146)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 351 total aligned reads displayed.

GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAT  >  NC_000913/3082871‑3083050
                                                                                          |                                                                                         
gCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCt                                                                                         <  2:1702892/93‑1 (MQ=255)
 cGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTg                                                                                        >  1:5217859/1‑93 (MQ=255)
  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTgg                                                                                       >  1:8196382/1‑92 (MQ=255)
  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGc                                                                                       <  1:119545/93‑1 (MQ=255)
  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGc                                                                                       <  1:9240934/93‑1 (MQ=255)
     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                    <  2:2446800/93‑1 (MQ=255)
     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                    <  2:6740544/93‑1 (MQ=255)
      gACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAc                                                                                   >  2:1560858/1‑93 (MQ=255)
      gACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAc                                                                                   >  1:1761753/1‑93 (MQ=255)
      gACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAc                                                                                   >  2:4805726/1‑93 (MQ=255)
      gACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGACGTAGTTCACCGAACAGTCAGGCTGCGAAc                                                                                   >  2:4150756/1‑93 (MQ=255)
       aCCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACg                                                                                  >  2:1309583/1‑93 (MQ=255)
       aCCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACg                                                                                  <  1:1444024/93‑1 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAga                                                                               >  2:7999669/1‑92 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGt                                                                               >  1:2052731/1‑93 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGt                                                                               <  2:2495786/93‑1 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGt                                                                               <  1:4862842/93‑1 (MQ=255)
            tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAc                                                                             <  1:102863/93‑1 (MQ=255)
            tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAc                                                                             <  2:4541167/93‑1 (MQ=255)
                 tcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCa                                                                        >  2:3697824/1‑93 (MQ=255)
                 tcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCa                                                                        <  2:9233538/93‑1 (MQ=255)
                   acacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATa                                                                      <  2:6382058/93‑1 (MQ=255)
                   acacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATa                                                                      >  2:7131597/1‑93 (MQ=255)
                    cacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATaa                                                                     >  2:3111078/1‑93 (MQ=255)
                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                    <  2:6278581/93‑1 (MQ=255)
                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                    <  1:5885316/93‑1 (MQ=255)
                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                    >  2:1237541/1‑93 (MQ=255)
                       gCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGTACGAGGACCTTCATAATcc                                                                  >  2:7847143/1‑93 (MQ=255)
                       gCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATcc                                                                  <  1:5125530/93‑1 (MQ=255)
                       gCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATcc                                                                  >  1:2437392/1‑93 (MQ=255)
                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                               >  2:7495040/1‑93 (MQ=255)
                           cGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGc                                                              <  2:5119503/93‑1 (MQ=255)
                            gCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGcc                                                             >  1:8914089/1‑93 (MQ=255)
                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                           >  2:8136901/1‑93 (MQ=255)
                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                          >  2:3181904/1‑93 (MQ=255)
                                 tCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAc                                                        >  2:1400470/1‑93 (MQ=255)
                                   gCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccg                                                      >  2:6603455/1‑93 (MQ=255)
                                     ccAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgcc                                                    <  2:9716857/93‑1 (MQ=255)
                                      cAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccg                                                   <  2:5010963/93‑1 (MQ=255)
                                      cAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccg                                                   >  2:769461/1‑93 (MQ=255)
                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  <  2:3493549/93‑1 (MQ=255)
                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  <  1:167684/93‑1 (MQ=255)
                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  <  1:3806294/93‑1 (MQ=255)
                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAGCAGTCGGGCTGCGAACGAGTACCTTCATAATACACGCCCAGACCGCCGCCGa                                               >  2:2617775/1‑93 (MQ=255)
                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGCCCACCGCCGa                                               <  2:6106189/93‑1 (MQ=255)
                                           aaTGTTGTGGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAc                                              <  1:4459432/93‑1 (MQ=255)
                                            aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACg                                             >  1:8459758/1‑93 (MQ=255)
                                             tgttgtTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGatgc                                            >  1:541333/1‑90 (MQ=255)
                                               ttgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCg                                          <  1:2615022/93‑1 (MQ=255)
                                               ttgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCg                                          <  2:3081369/93‑1 (MQ=255)
                                                 gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGaa                                        <  2:8429292/93‑1 (MQ=255)
                                                  ttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAg                                       >  1:5816386/1‑93 (MQ=255)
                                                   tgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGc                                      >  1:135768/1‑93 (MQ=255)
                                                   tgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGc                                      <  2:2074087/93‑1 (MQ=255)
                                                    ggCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa                                     >  1:7511206/1‑93 (MQ=255)
                                                     gCGTATTCATTGAGGCTGTAGTTCACCGAACAGTCGGGCGGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCTCCGACGTCGAAGCAc                                    <  2:545621/93‑1 (MQ=255)
                                                      cGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACt                                   <  2:5303670/93‑1 (MQ=255)
                                                      cGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACt                                   <  2:8540636/93‑1 (MQ=255)
                                                      cGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACt                                   >  2:3910142/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                 <  2:7580157/93‑1 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                 >  1:9506393/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                 >  1:4766353/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCCACGTCGAAGCACTGa                                 >  1:2311452/1‑93 (MQ=255)
                                                          ttCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAat                               >  1:9761769/1‑93 (MQ=255)
                                                           tccttGTGGCCGTAATTCACCCGACAGTCGGGCTGCGAACGAAGACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAata                              <  1:712546/90‑1 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                            >  1:9052563/1‑93 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                            >  1:6338015/1‑93 (MQ=255)
                                                              ttGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTg                           >  2:8877886/1‑93 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                          <  2:9468322/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                          <  1:7317185/93‑1 (MQ=255)
                                                                gAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAc                         >  2:5882400/1‑93 (MQ=255)
                                                                gAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATGGAc                         >  2:7333971/1‑93 (MQ=255)
                                                                  ggCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGc                       >  1:4236112/1‑93 (MQ=255)
                                                                  ggCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGc                       >  1:7768647/1‑93 (MQ=255)
                                                                  ggCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGc                       <  1:9397387/93‑1 (MQ=255)
                                                                   gCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCTCAGACCGCCGTCGACGTCGAAGCACTGAATATTGACGcc                      >  1:3133160/1‑93 (MQ=255)
                                                                   gCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGcc                      <  2:6484103/93‑1 (MQ=255)
                                                                    ccGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGccc                     <  1:5061872/93‑1 (MQ=255)
                                                                     cGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCa                    >  1:4332181/1‑93 (MQ=255)
                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCTGCCGACGTCGAAGCACTGAATATTGACGCCCAg                   >  1:9670718/1‑93 (MQ=255)
                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAg                   >  1:4607125/1‑93 (MQ=255)
                                                                         gTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCtt                <  1:6276681/93‑1 (MQ=255)
                                                                         gTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCtt                >  1:326498/1‑93 (MQ=255)
                                                                         gTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGAtt                >  1:3522777/1‑93 (MQ=255)
                                                                           tCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgt              <  2:1933175/93‑1 (MQ=255)
                                                                            cACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgtg             >  1:2165379/1‑93 (MQ=255)
                                                                            cACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgtg             <  1:815809/93‑1 (MQ=255)
                                                                            cACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgtg             >  1:289566/1‑93 (MQ=255)
                                                                             aCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGc            <  1:6210725/93‑1 (MQ=255)
                                                                              ccGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCa           <  2:8346663/93‑1 (MQ=255)
                                                                              ccGAACAGTAGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGACCAGCTCGTGCa           >  1:9893555/1‑93 (MQ=255)
                                                                               cGAACGGTCAGACTGCGAACGAGTACCTACATAATCCACGCCCAGACCTCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAg          <  2:356338/93‑1 (MQ=255)
                                                                               cGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                 <  1:5656268/38‑1 (MQ=255)
                                                                                gAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGt         >  1:3911488/1‑93 (MQ=255)
                                                                                 aaCAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGtt        >  1:8745113/1‑93 (MQ=255)
                                                                                  aCAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTc       >  2:224450/1‑93 (MQ=255)
                                                                                    aGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCca     >  2:5765118/1‑93 (MQ=255)
                                                                                     gTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcac    <  1:3571155/93‑1 (MQ=255)
                                                                                     gTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcac    >  1:6788450/1‑93 (MQ=255)
                                                                                      tCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcaca   >  2:2782108/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAT  >  NC_000913/3082871‑3083050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  >  NC_000913/3082869‑3083054
                                                                                            |                                                                                             
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGC                                                                                              <  SRR4302540.9687469/94‑1 (MQ=60)
 AGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCT                                                                                             <  SRR4302540.3665469/94‑1 (MQ=60)
  GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTG                                                                                            >  SRR4302540.5274737/1‑94 (MQ=60)
    GGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCG                                                                                          <  SRR4302540.120832/94‑1 (MQ=60)
    GGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCG                                                                                          <  SRR4302540.9351619/94‑1 (MQ=60)
    GGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCG                                                                                          >  SRR4302540.687947/1‑94 (MQ=60)
    GGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCG                                                                                          <  SRR4302540.1717125/94‑1 (MQ=60)
    GGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCG                                                                                          >  SRR4302540.3099849/1‑94 (MQ=60)
      CAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAA                                                                                        <  SRR4302540.2444997/94‑1 (MQ=60)
       AGACCGTTTTCTTCACACGGATCCCCAATTGCCCAGATAATGGTGTTGGCGGTTTCATTTAGGCCGTTGTTCCCCGAACAGTCGGGCTGCGAAC                                                                                       <  SRR4302540.1242375/94‑1 (MQ=42)
       AGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAC                                                                                       >  SRR4302540.991591/1‑94 (MQ=60)
       AGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAC                                                                                       <  SRR4302540.3069759/94‑1 (MQ=60)
       AGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAC                                                                                       >  SRR4302540.1780865/1‑94 (MQ=60)
        GACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACG                                                                                      >  SRR4302540.3233223/1‑94 (MQ=60)
        GACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTACTTCACCGAACAGTCGGGCTGCGAACG                                                                                      >  SRR4302540.9605233/1‑94 (MQ=60)
         ACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGA                                                                                     <  SRR4302540.1459720/94‑1 (MQ=60)
          CCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAG                                                                                    >  SRR4302540.4901859/1‑94 (MQ=60)
          CCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAG                                                                                    >  SRR4302540.2736625/1‑94 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGT                                                                                   <  SRR4302540.5992417/94‑1 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGT                                                                                   >  SRR4302540.2074832/1‑94 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGT                                                                                   >  SRR4302540.2074831/1‑94 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAAAAGTCGGGCTGCGAACGAGT                                                                                   >  SRR4302540.3651908/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302540.2325215/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  <  SRR4302540.4914991/94‑1 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302540.4220078/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302540.4318912/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302540.5157631/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302540.6506189/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  <  SRR4302540.9992691/94‑1 (MQ=60)
             TTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAC                                                                                 >  SRR4302540.135081/1‑94 (MQ=60)
             TTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGGAC                                                                                 >  SRR4302540.4506431/1‑94 (MQ=60)
              TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACC                                                                                <  SRR4302540.103961/94‑1 (MQ=60)
              TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACC                                                                                <  SRR4302540.5104820/94‑1 (MQ=60)
               TTCTTCACACGCATCGCCAATCGCCCAGATAATGATGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCT                                                                               >  SRR4302540.4160757/1‑94 (MQ=60)
                TCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTT                                                                              <  SRR4302540.8764911/94‑1 (MQ=60)
                 CTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTC                                                                             <  SRR4302540.8541902/94‑1 (MQ=60)
                  TTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGCGTACCTTCA                                                                            >  SRR4302540.5163175/1‑94 (MQ=60)
                  TTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCA                                                                            >  SRR4302540.1834691/1‑94 (MQ=60)
                   TCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCAT                                                                           <  SRR4302540.44680/94‑1 (MQ=60)
                   TCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGCACCTTCAT                                                                           >  SRR4302540.1248013/1‑94 (MQ=60)
                    CCCACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATA                                                                          <  SRR4302540.5895092/94‑1 (MQ=60)
                    CACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATA                                                                          <  SRR4302540.5895093/94‑1 (MQ=60)
                    CACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATA                                                                          >  SRR4302540.1496164/1‑94 (MQ=60)
                    CACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATA                                                                          >  SRR4302540.8301183/1‑94 (MQ=60)
                      CACGCATCGCCAATCTCCCAGATAATGTTGTTGGTGTATTCATTGAGGCCGTAGTTCACCGAATAGTCGGGCTGCGAACGAGTTCCTTCATAAT                                                                        >  SRR4302540.9069224/1‑94 (MQ=60)
                       ACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATC                                                                       <  SRR4302540.5950339/94‑1 (MQ=60)
                        CGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCC                                                                      >  SRR4302540.9482369/1‑94 (MQ=60)
                        CGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCC                                                                      >  SRR4302540.2463640/1‑94 (MQ=60)
                        CGCATCGCAAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCC                                                                      <  SRR4302540.9019075/94‑1 (MQ=60)
                         GCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCC                                                                     >  SRR4302540.4787238/1‑94 (MQ=60)
                         GCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCA                                                                     <  SRR4302540.5181042/94‑1 (MQ=60)
                          catacgagatcggtctcggcattcctgctgaaccgctcttccgatctagcgcttCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302540.5718550/40‑1 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302540.9469676/94‑1 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302540.469058/94‑1 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302540.2609221/94‑1 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302540.1043516/94‑1 (MQ=60)
                           ATCGCCAATCGCCCAGATACTGTTGTTGACGTATTCATTGAAGCCATAGTTCACCGAACAGTCGGGCTGCGAAAGAGTACCTACATAATCCACG                                                                   <  SRR4302540.9043501/94‑1 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 >  SRR4302540.5222440/1‑94 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 >  SRR4302540.9017321/1‑94 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              <  SRR4302540.6850292/94‑1 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              <  SRR4302540.6312611/94‑1 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              <  SRR4302540.337929/94‑1 (MQ=60)
                                   TCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACC                                                           <  SRR4302540.5196648/94‑1 (MQ=60)
                                     GCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGC                                                         >  SRR4302540.6570577/1‑94 (MQ=60)
                                      CCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCC                                                        <  SRR4302540.4223622/94‑1 (MQ=60)
                                      CCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCC                                                        <  SRR4302540.9705453/94‑1 (MQ=60)
                                        CAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGC                                                      >  SRR4302540.7520788/1‑94 (MQ=60)
                                        CAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGC                                                      >  SRR4302540.1470725/1‑94 (MQ=60)
                                         AGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCC                                                     <  SRR4302540.3847750/94‑1 (MQ=60)
                                         AGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCC                                                     <  SRR4302540.2023635/94‑1 (MQ=60)
                                         AGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCC                                                     <  SRR4302540.169368/94‑1 (MQ=60)
                                          GATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCG                                                    >  SRR4302540.8916945/1‑94 (MQ=60)
                                           ATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGA                                                   <  SRR4302540.9737633/94‑1 (MQ=60)
                                           ATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGA                                                   <  SRR4302540.7094784/94‑1 (MQ=60)
                                            TAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAC                                                  <  SRR4302540.3664826/94‑1 (MQ=60)
                                             AATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACG                                                 <  SRR4302540.2238025/94‑1 (MQ=60)
                                             AATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACG                                                 >  SRR4302540.8557979/1‑94 (MQ=60)
                                             AATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACG                                                 >  SRR4302540.8625345/1‑94 (MQ=60)
                                             AATGTTGTGGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACG                                                 <  SRR4302540.4507776/94‑1 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAtgc                                                >  SRR4302540.547193/1‑91 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGT                                                >  SRR4302540.547194/1‑94 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGT                                                >  SRR4302540.3601485/1‑94 (MQ=60)
                                               TGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTC                                               >  SRR4302540.570536/1‑94 (MQ=60)
                                                GTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCG                                              >  SRR4302540.9848370/1‑94 (MQ=60)
                                                 TTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGA                                             >  SRR4302540.9635273/1‑94 (MQ=60)
                                                 TTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGA                                             <  SRR4302540.2643268/94‑1 (MQ=60)
                                                  TGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAA                                            >  SRR4302540.1226603/1‑94 (MQ=60)
                                                   GTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGACG                                           >  SRR4302540.3014490/1‑94 (MQ=60)
                                                   GTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAG                                           >  SRR4302540.5880675/1‑94 (MQ=60)
                                                    TTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGC                                          >  SRR4302540.137190/1‑94 (MQ=60)
                                                     TGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA                                         <  SRR4302540.4878884/94‑1 (MQ=60)
                                                     TGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA                                         >  SRR4302540.7595166/1‑94 (MQ=60)
                                                     TGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA                                         <  SRR4302540.3561300/94‑1 (MQ=60)
                                                     TGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA                                         <  SRR4302540.3611703/94‑1 (MQ=60)
                                                      GGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCAC                                        >  SRR4302540.9957988/1‑94 (MQ=60)
                                                        CGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTG                                      >  SRR4302540.6344249/1‑94 (MQ=60)
                                                        CGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTG                                      >  SRR4302540.4490774/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302540.4817251/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302540.4689431/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302540.9619021/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCCACGTCGAAGCACTGA                                     >  SRR4302540.2336332/1‑94 (MQ=60)
                                                          TATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAA                                    <  SRR4302540.7397393/94‑1 (MQ=60)
                                                          TATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAA                                    >  SRR4302540.7680510/1‑94 (MQ=60)
                                                          TATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAA                                    >  SRR4302540.3317000/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302540.7766742/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302540.9876266/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACAGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302540.4574350/1‑94 (MQ=60)
                                                            TTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATA                                  >  SRR4302540.1483152/1‑94 (MQ=60)
                                                            TTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATA                                  >  SRR4302540.6109159/1‑94 (MQ=60)
                                                            TTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATA                                  >  SRR4302540.7664465/1‑94 (MQ=60)
                                                             TCCTTGTGGCCGTAATTCACCCGACAGTCGGGCTGCGAACGAAGACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAT                                 <  SRR4302540.720200/94‑1 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302540.9161237/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302540.6408599/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302540.6408598/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302540.1188444/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302540.248362/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCagaacggcgcccaagtcggagccctggatattta                               >  SRR4302540.9376558/1‑60 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302540.5113445/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               <  SRR4302540.1409681/94‑1 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302540.5085958/1‑94 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302540.2388391/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302540.7398903/94‑1 (MQ=60)
                                                                  TAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACG                            <  SRR4302540.5182344/94‑1 (MQ=60)
                                                                   AGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGC                           >  SRR4302540.4282206/1‑94 (MQ=60)
                                                                   AGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGC                           >  SRR4302540.7859469/1‑94 (MQ=60)
                                                                    GGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCTCAGACCGCCGTCGACGTCGAAGCACTGAATATTGACGCC                          >  SRR4302540.3167345/1‑94 (MQ=60)
                                                                    GGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCC                          <  SRR4302540.9509202/94‑1 (MQ=60)
                                                                    GGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCC                          <  SRR4302540.3901910/94‑1 (MQ=60)
                                                                     GCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCC                         >  SRR4302540.5553187/1‑94 (MQ=60)
                                                                      CCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCTCCGCCGACGTCGAAGCACTGAATATTGACGCCCA                        <  SRR4302540.5809375/94‑1 (MQ=60)
                                                                      CCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCA                        >  SRR4302540.4379340/1‑94 (MQ=60)
                                                                      CCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCA                        <  SRR4302540.5116598/94‑1 (MQ=60)
                                                                       CGTAGTTCACGGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGCCCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       <  SRR4302540.956608/94‑1 (MQ=60)
                                                                       CGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCTGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       >  SRR4302540.9784529/1‑94 (MQ=60)
                                                                       CGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       <  SRR4302540.754273/94‑1 (MQ=60)
                                                                       CGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       >  SRR4302540.4656604/1‑94 (MQ=60)
                                                                        GTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGC                      >  SRR4302540.3342815/1‑94 (MQ=60)
                                                                         TAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCT                     <  SRR4302540.9162828/94‑1 (MQ=60)
                                                                          AGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTT                    >  SRR4302540.170792/1‑94 (MQ=60)
                                                                          AGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTT                    <  SRR4302540.4656017/94‑1 (MQ=60)
                                                                          AGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTT                    >  SRR4302540.329770/1‑94 (MQ=60)
                                                                          AGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGATT                    >  SRR4302540.3561179/1‑94 (MQ=60)
                                                                           GTTCACGGAACAGTCGGGTGGCGAACGAGTACCTTCAGAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTG                   <  SRR4302540.6346510/94‑1 (MQ=60)
                                                                           GTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTG                   >  SRR4302540.224049/1‑94 (MQ=60)
                                                                           GTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTG                   >  SRR4302540.8849416/1‑94 (MQ=60)
                                                                           GTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTG                   <  SRR4302540.6346509/94‑1 (MQ=60)
                                                                             TCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTG                 >  SRR4302540.292426/1‑94 (MQ=60)
                                                                             TCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTG                 >  SRR4302540.2188701/1‑94 (MQ=60)
                                                                              CACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGC                >  SRR4302540.7991246/1‑94 (MQ=60)
                                                                              CACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGC                <  SRR4302540.824477/94‑1 (MQ=60)
                                                                              CACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGC                >  SRR4302540.14252/1‑94 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               >  SRR4302540.8042882/1‑94 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               <  SRR4302540.6279841/94‑1 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               >  SRR4302540.535534/1‑94 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               >  SRR4302540.8617280/1‑94 (MQ=60)
                                                                               ACCGAACAGTAGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGACCAGCTCGTGCA               >  SRR4302540.10009088/1‑94 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              <  SRR4302540.7225871/94‑1 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              <  SRR4302540.5863706/94‑1 (MQ=60)
                                                                                CCGAACAGTCGGGCGGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              <  SRR4302540.2471599/94‑1 (MQ=60)
                                                                                 CGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             >  SRR4302540.2742732/1‑94 (MQ=60)
                                                                                 CGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             >  SRR4302540.9839642/1‑94 (MQ=60)
                                                                                 CGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             >  SRR4302540.3953868/1‑94 (MQ=60)
                                                                                 CGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             >  SRR4302540.4346194/1‑94 (MQ=60)
                                                                                  GAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCTAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTT            <  SRR4302540.3905762/94‑1 (MQ=60)
                                                                                  GAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTT            >  SRR4302540.827023/1‑94 (MQ=60)
                                                                                  GAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTT            >  SRR4302540.8265170/1‑94 (MQ=60)
                                                                                  GAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTT            >  SRR4302540.8265169/1‑94 (MQ=60)
                                                                                  GAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTT            >  SRR4302540.8846605/1‑94 (MQ=60)
                                                                                   AACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTC           <  SRR4302540.3217230/94‑1 (MQ=60)
                                                                                    ACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCC          <  SRR4302540.7364947/94‑1 (MQ=60)
                                                                                    ACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCC          <  SRR4302540.5467650/94‑1 (MQ=60)
                                                                                     CAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCA         <  SRR4302540.2161789/94‑1 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCAC        >  SRR4302540.5885517/1‑94 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCAC        >  SRR4302540.6863519/1‑94 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCAC        <  SRR4302540.9275527/94‑1 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCACCTTGTGCAGTTCCAC        >  SRR4302540.6303378/1‑94 (MQ=60)
                                                                                       GTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACA       <  SRR4302540.3610163/94‑1 (MQ=60)
                                                                                         CGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATA     >  SRR4302540.5742739/1‑94 (MQ=60)
                                                                                         CGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATA     <  SRR4302540.1796920/94‑1 (MQ=60)
                                                                                          GGGCTGCGAACGAGTACCTTCATAATCCACCCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAG    <  SRR4302540.3666307/94‑1 (MQ=60)
                                                                                           GGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGA   <  SRR4302540.814113/94‑1 (MQ=60)
                                                                                            GCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  >  SRR4302540.8818996/1‑94 (MQ=60)
                                                                                            |                                                                                             
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  >  NC_000913/3082869‑3083054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑