Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,105,185 T→C E663E (GAA→GAG)  b2965 ← ornithine decarboxylase isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,1850TC94.2% 777.0 / 27.2 277E663E (GAA→GAG) b2965ornithine decarboxylase isozyme
Reads supporting (aligned to +/- strand):  ref base T (8/8);  new base C (118/143);  total (126/151)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 289 total aligned reads displayed.

AAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTC  >  NC_000913/3105094‑3105277
                                                                                           |                                                                                            
aaaCACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACtt                                                                                             >  2:9714120/1‑93 (MQ=255)
  acacCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTcc                                                                                           <  1:5369585/93‑1 (MQ=255)
   cacCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTccc                                                                                          <  1:7402574/93‑1 (MQ=255)
     ccTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCg                                                                                        >  1:7350055/1‑93 (MQ=255)
     ccTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCg                                                                                        <  2:5318123/93‑1 (MQ=255)
     ccTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCg                                                                                        >  2:7167286/1‑93 (MQ=255)
       ttGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCggg                                                                                      >  2:5869861/1‑93 (MQ=255)
       ctGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCggg                                                                                      <  1:2954931/92‑1 (MQ=255)
        tGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGt                                                                                     <  2:3620754/93‑1 (MQ=255)
         gCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTa                                                                                    <  1:1675530/93‑1 (MQ=255)
         gCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTa                                                                                    >  1:4221530/1‑93 (MQ=255)
         gCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTa                                                                                    <  2:7765772/93‑1 (MQ=255)
         gCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGCAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTa                                                                                    <  2:9903020/93‑1 (MQ=255)
           aGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTAcc                                                                                  <  1:2171903/93‑1 (MQ=255)
            gCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCa                                                                                 <  2:4646386/93‑1 (MQ=255)
            gCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCa                                                                                 >  1:5467095/1‑93 (MQ=255)
            gCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCa                                                                                 >  1:701802/1‑93 (MQ=255)
               ccGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGc                                                                              <  1:5755042/93‑1 (MQ=255)
                 ggCGAAAATCCCGGCAACAAATTGACGCCTTCTTCCAGTGCAAGGAAATAACGTTGAACGGTGCCACCCCAGACCTCCCCGGGTACCACGCaa                                                                            >  2:1107911/1‑93 (MQ=255)
                 ggCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCaa                                                                            >  1:7548676/1‑93 (MQ=255)
                 ggCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCaa                                                                            <  1:6371875/93‑1 (MQ=255)
                  gCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCaaa                                                                           <  1:3837832/93‑1 (MQ=255)
                  gCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCaaa                                                                           <  2:9572709/93‑1 (MQ=255)
                    gAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGc                                                                         >  1:170776/1‑93 (MQ=255)
                    gAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGc                                                                         >  1:5124632/1‑93 (MQ=255)
                     aaaaTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCa                                                                        >  1:5234724/1‑93 (MQ=255)
                     aaaaTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCa                                                                        <  2:2148461/93‑1 (MQ=255)
                     aaaaTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCa                                                                        <  2:8186380/93‑1 (MQ=255)
                     aaaaTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGCGAATACCGTTGAACCGCCCCACCCCAGACCCCCCCGGGTACCACGCAAAGCa                                                                        <  2:1738845/93‑1 (MQ=255)
                     aaaaTCCCGGCAACAAAGTCACCCCGTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCa                                                                        <  2:4416378/93‑1 (MQ=255)
                        aTCCCGGCAACAAATTCCCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGc                                                                     <  2:8736486/93‑1 (MQ=255)
                        aTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGc                                                                     <  1:4795398/93‑1 (MQ=255)
                        aTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGc                                                                     >  2:839520/1‑93 (MQ=255)
                          cccGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCa                                                                   <  1:940672/93‑1 (MQ=255)
                            cGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAgg                                                                 >  1:3954627/1‑93 (MQ=255)
                            cGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAgg                                                                 <  2:904926/93‑1 (MQ=255)
                                 acaaATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGAt                                                            <  2:1324690/93‑1 (MQ=255)
                                  aaaaTTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATa                                                           <  2:6334777/92‑1 (MQ=255)
                                   aaaTTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATaa                                                          <  2:6212392/93‑1 (MQ=255)
                                   aaaTTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATaa                                                          >  2:9366234/1‑93 (MQ=255)
                                    aaTTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAg                                                         <  2:2945450/93‑1 (MQ=255)
                                      ttCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCGCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGc                                                       <  1:1824630/93‑1 (MQ=255)
                                       tCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCa                                                      >  1:10033625/1‑93 (MQ=255)
                                        ctccccTCCTTCAAGTGCAAGAAAATAGCGTTGAGCCGCCCCGCCCGAGACCGCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCaa                                                     <  2:3877399/91‑1 (MQ=255)
                                        cACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCaa                                                     <  2:4904801/93‑1 (MQ=255)
                                        cACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCaa                                                     <  1:4377387/93‑1 (MQ=255)
                                         cccccTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAc                                                    <  1:9308025/92‑1 (MQ=255)
                                         cccccTTCTTCCAGTGCAAGGAAATAACCTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAc                                                    <  2:8252733/92‑1 (MQ=255)
                                            ccTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGcc                                                 <  2:2477338/93‑1 (MQ=255)
                                            ccTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGcc                                                 <  1:2878028/93‑1 (MQ=255)
                                            ccTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGcc                                                 <  2:5352324/93‑1 (MQ=255)
                                             cttcttCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGTTGGATAAGGCAACGccc                                                >  1:8989075/1‑93 (MQ=255)
                                              ttcttcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGcccc                                               >  1:8293330/1‑93 (MQ=255)
                                               tcttcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCt                                              >  1:9888044/1‑93 (MQ=255)
                                                 ttcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTc                                            <  1:4908432/93‑1 (MQ=255)
                                                 ttcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTc                                            <  1:198521/93‑1 (MQ=255)
                                                  tcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCt                                           >  1:1132495/1‑93 (MQ=255)
                                                  tcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCt                                           <  1:1413036/93‑1 (MQ=255)
                                                  tcCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCt                                           <  1:3191553/93‑1 (MQ=255)
                                                  tcCAGTGCAAGGAAATAACGTGGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACCCCAGGTGGATAAGGCAACGCCCCTTCt                                           <  2:6907539/93‑1 (MQ=255)
                                                   ccAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTg                                          >  2:6766987/1‑93 (MQ=255)
                                                    cAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGc                                         <  2:2694123/93‑1 (MQ=255)
                                                     aGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGcc                                        <  1:6917452/93‑1 (MQ=255)
                                                     aGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACAACGCAAGGCAGCCCGGGGGGATAAGGCAAGGCCCCTTCTccc                                        >  1:5897450/1‑93 (MQ=255)
                                                        gCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATGAGGCAACGCCCCTTCTCCCGCa                                     >  1:9106861/1‑93 (MQ=255)
                                                          aaGAAAATACCGTTGACCCGCCCCACCCCAGCCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAAt                                   <  2:1903891/93‑1 (MQ=255)
                                                             gAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCg                                >  1:3648443/1‑93 (MQ=255)
                                                               aaTAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGcc                              <  1:1365886/93‑1 (MQ=255)
                                                                 aaaCGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCt                            <  1:7968821/92‑1 (MQ=255)
                                                                   aCGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTc                          <  2:7861213/93‑1 (MQ=255)
                                                                    cGTTGAACCGCCCCCCCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGTTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCg                         <  2:3468625/93‑1 (MQ=255)
                                                                     gtggAACCCCCCCCCCCCAGTCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCgg                        <  2:7320471/90‑1 (MQ=255)
                                                                     gTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCgg                        >  1:8833480/1‑93 (MQ=255)
                                                                     gTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCgg                        >  1:2389937/1‑93 (MQ=255)
                                                                      ttGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGc                       >  1:3362075/1‑93 (MQ=255)
                                                                       tGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCa                      >  1:8211514/1‑93 (MQ=255)
                                                                         aaCCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATc                    >  1:1939063/1‑93 (MQ=255)
                                                                          aCCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATcc                   >  2:3167237/1‑92 (MQ=255)
                                                                          aCCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCa                   <  1:7883941/93‑1 (MQ=255)
                                                                          aCCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCa                   <  2:4247028/93‑1 (MQ=255)
                                                                             gCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                >  1:7193745/1‑93 (MQ=255)
                                                                             gCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                >  1:6706918/1‑93 (MQ=255)
                                                                             gCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                >  2:7470177/1‑93 (MQ=255)
                                                                             gCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                >  2:8652470/1‑93 (MQ=255)
                                                                             ccccccccccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                <  2:8453378/92‑1 (MQ=255)
                                                                             cccccaccccaGCCCCCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGa                <  2:8061165/92‑1 (MQ=255)
                                                                               cccaccccaGCCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAAt              >  2:6547113/1‑93 (MQ=255)
                                                                                ccaccccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATc             <  2:1444060/93‑1 (MQ=255)
                                                                                 caccccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATcc            >  2:1151306/1‑93 (MQ=255)
                                                                                 caccccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGAATAAGGCAACGCACCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATcc            <  1:8064810/93‑1 (MQ=255)
                                                                                    cccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCa         <  1:260921/93‑1 (MQ=255)
                                                                                    cccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCa         >  1:660262/1‑93 (MQ=255)
                                                                                    cccaGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCa         <  1:3444891/93‑1 (MQ=255)
                                                                                      caGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCAcc       >  2:397505/1‑93 (MQ=255)
                                                                                       aGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCa      >  2:3295108/1‑93 (MQ=255)
                                                                                        gACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCaa     <  1:843638/93‑1 (MQ=255)
                                                                                        gACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCaa     <  1:9701414/93‑1 (MQ=255)
                                                                                        gACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCaa     >  1:2127174/1‑93 (MQ=255)
                                                                                        gACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCaa     >  1:5541788/1‑93 (MQ=255)
                                                                                           ttCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTc  <  2:3657189/93‑1 (MQ=255)
                                                                                           |                                                                                            
AAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTC  >  NC_000913/3105094‑3105277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTCC  >  NC_000913/3105092‑3105278
                                                                                             |                                                                                             
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACC                                                                                               >  SRR4302540.8881052/1‑94 (MQ=60)
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACC                                                                                               >  SRR4302540.1324430/1‑94 (MQ=60)
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACC                                                                                               <  SRR4302540.6242477/94‑1 (MQ=60)
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACC                                                                                               >  SRR4302540.4107102/1‑94 (MQ=60)
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACC                                                                                               <  SRR4302540.2193595/94‑1 (MQ=60)
 TAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCT                                                                                              <  SRR4302540.1027039/94‑1 (MQ=60)
  AAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTC                                                                                             <  SRR4302540.8920637/94‑1 (MQ=60)
  AAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTC                                                                                             >  SRR4302540.525656/1‑94 (MQ=60)
  AAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTC                                                                                             <  SRR4302540.7255501/94‑1 (MQ=60)
    ACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCC                                                                                           <  SRR4302540.5428599/94‑1 (MQ=60)
     CACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGCAATAACGTTGAACCGCCCCACCCCAGACCTCCCC                                                                                          >  SRR4302540.2921838/1‑94 (MQ=60)
     CACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCC                                                                                          <  SRR4302540.7485045/94‑1 (MQ=60)
     CACCTTGCAGCTCCGGCGAAAATCCCGGCAAAAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCC                                                                                          <  SRR4302540.8059130/94‑1 (MQ=60)
      ACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCG                                                                                         >  SRR4302540.7432049/1‑94 (MQ=60)
      ACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCG                                                                                         >  SRR4302540.8830951/1‑94 (MQ=60)
      ACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCG                                                                                         <  SRR4302540.5125180/94‑1 (MQ=60)
       CCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCGCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGG                                                                                        <  SRR4302540.7992047/94‑1 (MQ=60)
        CTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGG                                                                                       >  SRR4302540.574907/1‑94 (MQ=60)
         TTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGT                                                                                      >  SRR4302540.1929667/1‑94 (MQ=60)
         TTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGT                                                                                      <  SRR4302540.1952318/94‑1 (MQ=60)
         CTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGT                                                                                      <  SRR4302540.2987477/94‑1 (MQ=60)
          TGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTA                                                                                     >  SRR4302540.4267489/1‑94 (MQ=60)
          TGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTA                                                                                     >  SRR4302540.7104906/1‑94 (MQ=60)
           GCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTAC                                                                                    <  SRR4302540.1693731/94‑1 (MQ=60)
           GCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTAC                                                                                    <  SRR4302540.8150544/94‑1 (MQ=60)
            CAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACC                                                                                   >  SRR4302540.6593752/1‑94 (MQ=60)
             AGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCA                                                                                  >  SRR4302540.5527274/1‑94 (MQ=60)
             AGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCA                                                                                  >  SRR4302540.709336/1‑94 (MQ=60)
             AGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCA                                                                                  <  SRR4302540.2195324/94‑1 (MQ=60)
               CTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACG                                                                                <  SRR4302540.3454508/94‑1 (MQ=60)
               CTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACG                                                                                >  SRR4302540.171564/1‑94 (MQ=60)
                 CCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCA                                                                              >  SRR4302540.2094061/1‑94 (MQ=60)
                 CCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCA                                                                              <  SRR4302540.5818571/94‑1 (MQ=60)
                  CGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAA                                                                             >  SRR4302540.7633285/1‑94 (MQ=60)
                   GGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAATCGTCCCACCCCAGACCTCCCCGGGTACCACGCAAA                                                                            >  SRR4302540.9241383/1‑94 (MQ=60)
                   GGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAA                                                                            <  SRR4302540.6442824/94‑1 (MQ=60)
                    GCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAG                                                                           <  SRR4302540.3879573/94‑1 (MQ=60)
                     CTAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGC                                                                          <  SRR4302540.3099327/94‑1 (MQ=60)
                     CGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGC                                                                          >  SRR4302540.172486/1‑94 (MQ=60)
                     CGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGC                                                                          >  SRR4302540.5180138/1‑94 (MQ=60)
                      GAAAATCCCGGCAGCAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGCCCTCCCCGGGTACCACGCAAAGCA                                                                         <  SRR4302540.2021899/94‑1 (MQ=60)
                      GAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCA                                                                         >  SRR4302540.4952607/1‑94 (MQ=60)
                      GAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCA                                                                         >  SRR4302540.5291831/1‑94 (MQ=60)
                       AAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCAC                                                                        <  SRR4302540.6481933/94‑1 (MQ=60)
                       AAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCAC                                                                        <  SRR4302540.9457917/94‑1 (MQ=60)
                       AAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCAC                                                                        <  SRR4302540.7460914/94‑1 (MQ=60)
                         AATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGC                                                                      >  SRR4302540.4564454/1‑94 (MQ=60)
                         AATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGC                                                                      >  SRR4302540.7390053/1‑94 (MQ=60)
                         AATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGC                                                                      <  SRR4302540.4968835/94‑1 (MQ=60)
                          ATCCCGGCACCAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCC                                                                     <  SRR4302540.9034651/94‑1 (MQ=60)
                          ATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCC                                                                     <  SRR4302540.4846699/94‑1 (MQ=60)
                          ATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCC                                                                     <  SRR4302540.6968901/94‑1 (MQ=60)
                            CCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAG                                                                   <  SRR4302540.950503/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  <  SRR4302540.8208611/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  <  SRR4302540.8454610/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  <  SRR4302540.6689366/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  <  SRR4302540.7478046/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  <  SRR4302540.3744119/94‑1 (MQ=60)
                             CCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGG                                                                  >  SRR4302540.3997305/1‑94 (MQ=60)
                              CGGCAACAAATTCGCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGT                                                                 <  SRR4302540.3276492/94‑1 (MQ=60)
                               GGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTG                                                                <  SRR4302540.5864747/94‑1 (MQ=60)
                                  AACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGAT                                                             >  SRR4302540.1207963/1‑94 (MQ=60)
                                   ACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATA                                                            <  SRR4302540.9908849/94‑1 (MQ=60)
                                    CAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAA                                                           <  SRR4302540.1347527/94‑1 (MQ=60)
                                     AAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAG                                                          <  SRR4302540.8359777/94‑1 (MQ=60)
                                        TTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCA                                                       >  SRR4302540.10150330/1‑94 (MQ=60)
                                        TTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCGCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCA                                                       <  SRR4302540.1844577/94‑1 (MQ=60)
                                          CACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAC                                                     >  SRR4302540.6737456/1‑94 (MQ=60)
                                          CACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAC                                                     <  SRR4302540.5722103/94‑1 (MQ=60)
                                          CACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAC                                                     <  SRR4302540.4424918/94‑1 (MQ=60)
                                          CACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAC                                                     <  SRR4302540.6161417/94‑1 (MQ=60)
                                           CCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    <  SRR4302540.9419162/94‑1 (MQ=60)
                                           CCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    <  SRR4302540.9812457/94‑1 (MQ=60)
                                           CCCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    <  SRR4302540.7607188/94‑1 (MQ=60)
                                           CCCCCTTCTTCCAGTGAAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    <  SRR4302540.3667101/94‑1 (MQ=60)
                                           CCCCCCTCTTCCAGTGAAATGAAATAACGTTGAACCGCCCCACCCCAGACCGCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    <  SRR4302540.3667100/94‑1 (MQ=60)
                                           ACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACG                                                    >  SRR4302540.3445499/1‑94 (MQ=60)
                                            CCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGC                                                   <  SRR4302540.2485839/94‑1 (MQ=60)
                                             CCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCA                                                  >  SRR4302540.3945653/1‑94 (MQ=60)
                                              CCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGTTGGATAAGGCAACGCCC                                                 >  SRR4302540.9096615/1‑94 (MQ=60)
                                              CCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCC                                                 <  SRR4302540.2909328/94‑1 (MQ=60)
                                               CTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCC                                                <  SRR4302540.9835529/94‑1 (MQ=60)
                                               CTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCC                                                >  SRR4302540.8389634/1‑94 (MQ=60)
                                               CTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCC                                                >  SRR4302540.8389631/1‑94 (MQ=60)
                                               CTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCC                                                <  SRR4302540.5553044/94‑1 (MQ=60)
                                                ttcgtgcagtgcaagcaaagacgGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               <  SRR4302540.5535313/71‑1 (MQ=60)
                                                TTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               >  SRR4302540.10003500/1‑94 (MQ=60)
                                                TTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               <  SRR4302540.4589407/94‑1 (MQ=60)
                                                TTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               <  SRR4302540.5535312/94‑1 (MQ=60)
                                                TTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               >  SRR4302540.827028/1‑94 (MQ=60)
                                                TTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCT                                               >  SRR4302540.2193080/1‑94 (MQ=60)
                                                 TCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTT                                              >  SRR4302540.687622/1‑94 (MQ=60)
                                                 TCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTT                                              >  SRR4302540.1390563/1‑94 (MQ=60)
                                                 TCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTT                                              >  SRR4302540.1390564/1‑94 (MQ=60)
                                                 TCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTT                                              <  SRR4302540.5361796/94‑1 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            <  SRR4302540.4961273/94‑1 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            <  SRR4302540.200485/94‑1 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            >  SRR4302540.1144289/1‑94 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            <  SRR4302540.1752887/94‑1 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            <  SRR4302540.200486/94‑1 (MQ=60)
                                                   TTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCT                                            <  SRR4302540.8965898/94‑1 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           >  SRR4302540.1714658/1‑94 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           <  SRR4302540.1428279/94‑1 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           <  SRR4302540.2831610/94‑1 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           <  SRR4302540.3226220/94‑1 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           <  SRR4302540.1027707/94‑1 (MQ=60)
                                                    TCCAGTGCAAGGAAATAACGTAGAACCTCCCCCCCCCAGTCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTG                                           <  SRR4302540.9129974/94‑1 (MQ=60)
                                                      CAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACAACGCAAGGCAGCCCGGGGGGATAAGGCAAGGCCCCTTCTCCC                                         >  SRR4302540.5962580/1‑94 (MQ=57)
                                                       AGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCG                                        <  SRR4302540.6994427/94‑1 (MQ=60)
                                                         TGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATGAGGCAACGCCCCTTCTCCCGCA                                      >  SRR4302540.9216302/1‑94 (MQ=60)
                                                           CAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAAT                                    <  SRR4302540.7663476/94‑1 (MQ=60)
                                                           CAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAAT                                    <  SRR4302540.4332535/94‑1 (MQ=60)
                                                           CAAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAAT                                    >  SRR4302540.3038106/1‑94 (MQ=60)
                                                            AAGGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATT                                   >  SRR4302540.1574391/1‑94 (MQ=60)
                                                              GGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCG                                 >  SRR4302540.3688408/1‑94 (MQ=60)
                                                              GGAAAGAACGTTGAACCGCCCCACCCCAGCCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCG                                 <  SRR4302540.6055365/94‑1 (MQ=60)
                                                              AGAAATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCG                                 <  SRR4302540.8677554/94‑1 (MQ=60)
                                                                 AATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCC                              <  SRR4302540.1380334/94‑1 (MQ=60)
                                                                  ATAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCT                             <  SRR4302540.1691975/94‑1 (MQ=60)
                                                                   AAACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTT                            <  SRR4302540.8061681/94‑1 (MQ=60)
                                                                    AACGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTC                           <  SRR4302540.9241633/94‑1 (MQ=60)
                                                                      CGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGG                         >  SRR4302540.8935647/1‑94 (MQ=60)
                                                                      CGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGG                         >  SRR4302540.2415696/1‑94 (MQ=60)
                                                                      CGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGG                         >  SRR4302540.2515752/1‑94 (MQ=60)
                                                                      CGTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGG                         >  SRR4302540.9444498/1‑94 (MQ=60)
                                                                       GTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGC                        >  SRR4302540.5516398/1‑94 (MQ=60)
                                                                       GTTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGC                        >  SRR4302540.3398874/1‑94 (MQ=60)
                                                                        TTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCA                       >  SRR4302540.8306958/1‑94 (MQ=60)
                                                                        TTGAACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGCCA                       >  SRR4302540.7766389/1‑94 (MQ=60)
                                                                          GAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATC                     >  SRR4302540.1960180/1‑94 (MQ=60)
                                                                           AACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCA                    <  SRR4302540.2903110/94‑1 (MQ=60)
                                                                            ACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCAC                   <  SRR4302540.7975992/94‑1 (MQ=60)
                                                                            ACCGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCAC                   >  SRR4302540.7346095/1‑94 (MQ=60)
                                                                             CCGCCCCCCCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTTGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACG                  <  SRR4302540.1975081/94‑1 (MQ=60)
                                                                              CGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGA                 >  SRR4302540.3999071/1‑94 (MQ=60)
                                                                              CGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGA                 >  SRR4302540.7273930/1‑94 (MQ=60)
                                                                              CGCCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGA                 >  SRR4302540.6780936/1‑94 (MQ=60)
                                                                                CCCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAAT               >  SRR4302540.9541351/1‑94 (MQ=60)
                                                                                 CCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATC              >  SRR4302540.7496274/1‑94 (MQ=60)
                                                                                 CCCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATC              <  SRR4302540.4034793/94‑1 (MQ=60)
                                                                                  CCGCCCCAGACTTCCCCGGGTACCGCGCAAAGCACGCTAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCC             <  SRR4302540.10023415/94‑1 (MQ=60)
                                                                                  CCACCCCAGCCCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCC             <  SRR4302540.7069649/94‑1 (MQ=60)
                                                                                  CCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCC             >  SRR4302540.2349738/1‑94 (MQ=60)
                                                                                  CCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCC             >  SRR4302540.4910454/1‑94 (MQ=60)
                                                                                  CCACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCC             >  SRR4302540.5257040/1‑94 (MQ=60)
                                                                                  CCACCCCAGACCTCCCCGGGAACCCCGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCG             >  SRR4302540.2349737/1‑94 (MQ=60)
                                                                                   CACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGAATAAGGCAACGCACCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCG            <  SRR4302540.8158406/94‑1 (MQ=60)
                                                                                    ACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGC           >  SRR4302540.1795657/1‑94 (MQ=60)
                                                                                    ACCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGC           >  SRR4302540.7025241/1‑94 (MQ=60)
                                                                                     CCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCA          <  SRR4302540.3922183/94‑1 (MQ=60)
                                                                                     CCCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCA          >  SRR4302540.667327/1‑94 (MQ=60)
                                                                                      CCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCAC         <  SRR4302540.916742/94‑1 (MQ=60)
                                                                                      CCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCAC         <  SRR4302540.3482355/94‑1 (MQ=60)
                                                                                      CCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCAC         <  SRR4302540.263467/94‑1 (MQ=60)
                                                                                      CCCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCAC         >  SRR4302540.295033/1‑94 (MQ=60)
                                                                                       CCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACC        >  SRR4302540.541689/1‑94 (MQ=60)
                                                                                       CCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACC        <  SRR4302540.4417396/94‑1 (MQ=60)
                                                                                       CCAGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACC        >  SRR4302540.5960478/1‑94 (MQ=60)
                                                                                         AGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAA      >  SRR4302540.8100339/1‑94 (MQ=60)
                                                                                         AGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAA      >  SRR4302540.2150000/1‑94 (MQ=60)
                                                                                         AGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAA      >  SRR4302540.3375820/1‑94 (MQ=60)
                                                                                         AGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAA      >  SRR4302540.5602826/1‑94 (MQ=60)
                                                                                         AGACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGCATAAGGCACCGCCCCTCCTGCTGCAATTAGACCTTCGCCATCACGAATCCGCACCAA      >  SRR4302540.3376015/1‑94 (MQ=60)
                                                                                          GACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAAC     <  SRR4302540.852582/94‑1 (MQ=60)
                                                                                          GACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAAC     <  SRR4302540.9815468/94‑1 (MQ=60)
                                                                                           ACCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACC    >  SRR4302540.2538645/1‑94 (MQ=60)
                                                                                            CCTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTC   <  SRR4302540.2735279/94‑1 (MQ=60)
                                                                                             CTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTCC  >  SRR4302540.5203479/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCGCAATTCGCCCTTCGGCATCACGAATCCGCACCAACTCC  >  NC_000913/3105092‑3105278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑