Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,254 2 bp→GC intergenic (‑569/‑12) yhcC ← / → gltB hypothetical protein/glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,2540CG100.0% 1166.0 / NA 361intergenic (‑569/‑13)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (163/198);  total (163/198)
*NC_0009133,352,2550GC99.7% 1174.7 / NA 361intergenic (‑570/‑12)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (162/198);  minor base A (1/0);  total (163/198)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341
                                                                                       ||                                                                                      
agcagcCTGCTGATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACACCAAGGGGGCGAATGCGAgg                                                                                    >  2:2625674/1‑93 (MQ=255)
 gcagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgc                                                                                   >  2:3305496/1‑93 (MQ=255)
  cagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcg                                                                                  <  2:8028809/93‑1 (MQ=255)
   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc                                                                                 <  2:6706268/93‑1 (MQ=255)
    gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                >  1:2107672/1‑93 (MQ=255)
     ccTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc                                                                               <  2:3385880/93‑1 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGcgcgcga                                                                             >  2:1099507/1‑92 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  2:2570446/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  1:3149253/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  1:3438538/1‑93 (MQ=255)
        gCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                            <  2:4223220/93‑1 (MQ=255)
        gCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                            <  2:7914014/93‑1 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          <  2:8290748/93‑1 (MQ=255)
            atcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac                                                                        >  2:1924217/1‑93 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       <  2:5719241/93‑1 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       >  2:69036/1‑93 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       <  1:3338869/93‑1 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       >  1:859530/1‑93 (MQ=255)
              catATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGacac                                                                      <  2:5266442/93‑1 (MQ=255)
              catATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGacac                                                                      >  2:6638250/1‑93 (MQ=255)
                tatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc                                                                    >  2:8742674/1‑93 (MQ=255)
                tatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc                                                                    <  1:4420348/93‑1 (MQ=255)
                tatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc                                                                    >  1:2033545/1‑93 (MQ=255)
                  tttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaa                                                                  <  1:9975669/93‑1 (MQ=255)
                   ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa                                                                 <  2:7669034/93‑1 (MQ=255)
                   ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa                                                                 <  1:1002483/93‑1 (MQ=255)
                    tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAc                                                                <  1:9572423/93‑1 (MQ=255)
                     aTGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcc                                                               >  1:7203492/1‑93 (MQ=255)
                      tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                              <  2:3543216/93‑1 (MQ=255)
                      tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                              <  2:2350325/93‑1 (MQ=255)
                         aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt                                                           <  2:1023726/93‑1 (MQ=255)
                         aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt                                                           <  1:254475/93‑1 (MQ=255)
                          gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc                                                          >  2:4330977/1‑93 (MQ=255)
                           tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCg                                                         >  2:7954030/1‑93 (MQ=255)
                            aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                        <  2:7139588/93‑1 (MQ=255)
                             aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACACGGGGGCGAATGCGAGGCGCGCGTATGACCCGCAAACCCCGTCGcc                                                       <  2:8953036/93‑1 (MQ=255)
                             aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCACACCCCGTCGcc                                                       >  1:2283595/1‑93 (MQ=255)
                              ttGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa                                                      <  2:3244288/93‑1 (MQ=255)
                                  gaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct                                                  >  2:84734/1‑93 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 <  1:9320372/93‑1 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 >  1:8851320/1‑93 (MQ=255)
                                   aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                                                 >  1:4907512/1‑93 (MQ=255)
                                    tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctct                                                <  1:1917677/93‑1 (MQ=255)
                                     ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt                                               <  1:5321600/93‑1 (MQ=255)
                                      cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt                                              <  2:1472974/93‑1 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             <  2:5218734/93‑1 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             <  2:9324514/93‑1 (MQ=255)
                                        ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                            >  2:9617433/1‑93 (MQ=255)
                                        ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                            <  1:4050209/93‑1 (MQ=255)
                                        ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                            <  1:639756/93‑1 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           >  1:8776568/1‑93 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGAGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           >  2:596975/1‑93 (MQ=255)
                                         tcgtCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           <  1:8432008/90‑1 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  2:3843567/93‑1 (MQ=255)
                                           ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgtg                                         >  1:5526804/1‑93 (MQ=255)
                                            tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGc                                        >  1:8538742/1‑93 (MQ=255)
                                            tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGc                                        <  2:883259/93‑1 (MQ=255)
                                             cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                                       <  1:395036/93‑1 (MQ=255)
                                             cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                                       >  1:6738620/1‑93 (MQ=255)
                                             cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                                       <  1:777106/93‑1 (MQ=255)
                                              aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                      <  1:5906796/93‑1 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   >  2:2991432/1‑93 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   >  2:1784167/1‑93 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   >  1:647302/1‑93 (MQ=255)
                                                  tATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCCCGCTCTTTCTGTGCCCgcgc                                  >  2:7485694/1‑93 (MQ=255)
                                                    ttAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                <  1:6622651/93‑1 (MQ=255)
                                                     tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                               >  1:6155061/1‑93 (MQ=255)
                                                     tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                               <  1:978418/93‑1 (MQ=255)
                                                       aCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc                             <  1:2435503/93‑1 (MQ=255)
                                                       aCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc                             <  1:4789228/93‑1 (MQ=255)
                                                        ccGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg                            <  1:3700813/93‑1 (MQ=255)
                                                        ccGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg                            <  1:4694667/93‑1 (MQ=255)
                                                          gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt                          >  1:3808576/1‑93 (MQ=255)
                                                          gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt                          >  1:4469324/1‑93 (MQ=255)
                                                           aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                         >  2:3636200/1‑93 (MQ=255)
                                                           aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                         >  2:2773638/1‑93 (MQ=255)
                                                           aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                         <  2:5907746/93‑1 (MQ=255)
                                                               gAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa                     <  2:5541598/93‑1 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    <  2:5026212/93‑1 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCCCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    <  1:1647606/93‑1 (MQ=255)
                                                                 aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                   <  2:823421/93‑1 (MQ=255)
                                                                 aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                   >  2:7855377/1‑93 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  2:8422339/1‑93 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  1:1864790/1‑93 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  1:5222282/1‑93 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  1:8249007/1‑93 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                <  2:1674071/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                >  1:7123467/1‑93 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTAGGAAGTgg                <  2:3108325/93‑1 (MQ=255)
                                                                     gACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTggg               >  2:6432318/1‑93 (MQ=255)
                                                                     gACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTggg               >  2:8442564/1‑93 (MQ=255)
                                                                      acaacaAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgggg              <  1:8336525/93‑1 (MQ=255)
                                                                        aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt            <  1:9446182/93‑1 (MQ=255)
                                                                        aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAAGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt            >  1:869917/1‑93 (MQ=255)
                                                                          caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTcc          <  1:797989/93‑1 (MQ=255)
                                                                            aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCg        >  1:2689195/1‑93 (MQ=255)
                                                                            aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCg        <  2:4045692/93‑1 (MQ=255)
                                                                             gggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGc       <  1:9240345/93‑1 (MQ=255)
                                                                              ggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa      <  2:8111857/93‑1 (MQ=255)
                                                                                ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCaga    <  1:1324227/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑CGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343
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TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑G                                                                                           <  SRR4302540.7656444/94‑1 (MQ=60)
   ATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGG                                                                                         >  SRR4302540.2833640/1‑94 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGG                                                                                      <  SRR4302540.3219911/94‑1 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGG                                                                                      <  SRR4302540.5885361/94‑1 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGG                                                                                      <  SRR4302540.4627608/94‑1 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGG                                                                                      <  SRR4302540.9329030/94‑1 (MQ=60)
     GAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGG                                                                                      <  SRR4302540.7817131/94‑1 (MQ=60)
       GCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCG                                                                                    <  SRR4302540.3860363/94‑1 (MQ=60)
       GCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCG                                                                                    >  SRR4302540.9821605/1‑94 (MQ=60)
       GCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCG                                                                                    >  SRR4302540.1473023/1‑94 (MQ=60)
       GCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCG                                                                                    <  SRR4302540.1030538/94‑1 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCG                                                                                  >  SRR4302540.2130292/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCG                                                                                  >  SRR4302540.8295779/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCG                                                                                  >  SRR4302540.7290904/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCG                                                                                  >  SRR4302540.1600950/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCG                                                                                  <  SRR4302540.3451026/94‑1 (MQ=60)
          GCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGC                                                                                 >  SRR4302540.7103813/1‑94 (MQ=60)
           CCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCG                                                                                >  SRR4302540.8651235/1‑94 (MQ=60)
           CCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCG                                                                                >  SRR4302540.9355133/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGT                                                                               >  SRR4302540.3475916/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGT                                                                               >  SRR4302540.6799250/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGT                                                                               <  SRR4302540.3968002/94‑1 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGT                                                                               >  SRR4302540.8593396/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGT                                                                               >  SRR4302540.204124/1‑94 (MQ=60)
             TGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTA                                                                              <  SRR4302540.4441176/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTAT                                                                             <  SRR4302540.8940412/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTAT                                                                             <  SRR4302540.10020636/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTAT                                                                             <  SRR4302540.7960984/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTAT                                                                             <  SRR4302540.9736618/94‑1 (MQ=60)
               CTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATG                                                                            <  SRR4302540.6589283/94‑1 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGA                                                                           >  SRR4302540.10101921/1‑94 (MQ=60)
                 CATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGAC                                                                          <  SRR4302540.207052/94‑1 (MQ=60)
                 CATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGAC                                                                          >  SRR4302540.3830143/1‑94 (MQ=60)
                  ATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACC                                                                         >  SRR4302540.8778161/1‑94 (MQ=60)
                  ATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACA                                                                         >  SRR4302540.9533288/1‑94 (MQ=60)
                  ATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACA                                                                         >  SRR4302540.868656/1‑94 (MQ=60)
                  ATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACA                                                                         >  SRR4302540.9532072/1‑94 (MQ=60)
                   TCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACAC                                                                        <  SRR4302540.3375526/94‑1 (MQ=60)
                   TCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACAC                                                                        <  SRR4302540.8821246/94‑1 (MQ=60)
                   TCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACAC                                                                        <  SRR4302540.8821239/94‑1 (MQ=60)
                    CATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACG                                                                       >  SRR4302540.6393147/1‑94 (MQ=60)
                    CATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACG                                                                       <  SRR4302540.2569836/94‑1 (MQ=60)
                    CATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACG                                                                       <  SRR4302540.3033962/94‑1 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGCGGCGCGCGTATGACACGC                                                                      >  SRR4302540.7973678/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302540.6797145/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302540.2055479/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGA                                                                      >  SRR4302540.2213763/1‑94 (MQ=60)
                      TATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCA                                                                     <  SRR4302540.3389791/94‑1 (MQ=60)
                      TATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCA                                                                     <  SRR4302540.6393840/94‑1 (MQ=60)
                      TATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCA                                                                     <  SRR4302540.4468243/94‑1 (MQ=60)
                       ATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAA                                                                    >  SRR4302540.6950372/1‑94 (MQ=60)
                        TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAA                                                                   <  SRR4302540.5542080/94‑1 (MQ=60)
                        TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAA                                                                   >  SRR4302540.207793/1‑94 (MQ=60)
                        TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAA                                                                   <  SRR4302540.10092009/94‑1 (MQ=60)
                         TTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAAC                                                                  <  SRR4302540.3808453/94‑1 (MQ=60)
                         TTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAAC                                                                  <  SRR4302540.1012665/94‑1 (MQ=60)
                          TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACC                                                                 >  SRR4302540.7283801/1‑94 (MQ=60)
                          TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACC                                                                 >  SRR4302540.5626032/1‑94 (MQ=60)
                          TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACC                                                                 >  SRR4302540.7425880/1‑94 (MQ=60)
                          TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACC                                                                 <  SRR4302540.9685506/94‑1 (MQ=60)
                           ATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCC                                                                >  SRR4302540.1759284/1‑94 (MQ=60)
                           ATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCC                                                                >  SRR4302540.1759283/1‑94 (MQ=60)
                           ATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCC                                                                >  SRR4302540.2492742/1‑94 (MQ=60)
                           ATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCC                                                                <  SRR4302540.3605689/94‑1 (MQ=60)
                            TGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCC                                                               >  SRR4302540.8983401/1‑94 (MQ=60)
                              CAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGT                                                             <  SRR4302540.8177366/94‑1 (MQ=60)
                              CAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGT                                                             <  SRR4302540.8656532/94‑1 (MQ=60)
                              CAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGT                                                             >  SRR4302540.8446516/1‑94 (MQ=60)
                              CAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGT                                                             <  SRR4302540.9413952/94‑1 (MQ=60)
                               AGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTC                                                            <  SRR4302540.256941/94‑1 (MQ=60)
                               AGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTC                                                            <  SRR4302540.9522046/94‑1 (MQ=60)
                                GTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCG                                                           <  SRR4302540.5538323/94‑1 (MQ=60)
                                 TAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGC                                                          <  SRR4302540.4594692/94‑1 (MQ=60)
                                  AATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCACACCCCGTCGCC                                                         >  SRR4302540.2308137/1‑94 (MQ=60)
                                  AATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCC                                                         <  SRR4302540.2642204/94‑1 (MQ=60)
                                   ATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCA                                                        <  SRR4302540.5791733/94‑1 (MQ=60)
                                   ATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCA                                                        <  SRR4302540.5791734/94‑1 (MQ=60)
                                   ATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCA                                                        >  SRR4302540.9051354/1‑94 (MQ=60)
                                    TTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAC                                                       <  SRR4302540.2328981/94‑1 (MQ=60)
                                    TTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAC                                                       >  SRR4302540.6510491/1‑94 (MQ=60)
                                    TTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAC                                                       >  SRR4302540.6510492/1‑94 (MQ=60)
                                    TTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAC                                                       <  SRR4302540.4703722/94‑1 (MQ=60)
                                       AGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCT                                                    <  SRR4302540.9639615/94‑1 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302540.3087098/1‑94 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302540.4960341/1‑94 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302540.8953715/1‑94 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  <  SRR4302540.9431577/94‑1 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  <  SRR4302540.8950420/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATTACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302540.2683481/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 >  SRR4302540.2148193/1‑94 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 >  SRR4302540.1859118/1‑94 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302540.1938602/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302540.7206169/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACACCGTCGCCACGCTCTT                                                 >  SRR4302540.6468026/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                <  SRR4302540.5379872/94‑1 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302540.8441257/1‑94 (MQ=60)
                                            CCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTC                                               >  SRR4302540.4571064/1‑94 (MQ=60)
                                             CCTCTTCACCGTATTAACCGCTGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              >  SRR4302540.4170664/1‑94 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302540.4936786/94‑1 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302540.3826681/94‑1 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302540.6007376/94‑1 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302540.8834598/94‑1 (MQ=60)
                                             CCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCT                                              <  SRR4302540.696458/94‑1 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             <  SRR4302540.646571/94‑1 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             >  SRR4302540.8878222/1‑94 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             >  SRR4302540.1273306/1‑94 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             <  SRR4302540.4093872/94‑1 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             <  SRR4302540.4490813/94‑1 (MQ=60)
                                               TCTTCACCGTATTAACAGATGCGAAAAGGAGAACAAGGGGTCGAAT‑GCGAGGCGCGCGTATGACACGCAACCCCCGTCGCCACGCTCTTTCTGT                                            <  SRR4302540.1122991/94‑1 (MQ=60)
                                               TCGTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGT                                            <  SRR4302540.8529893/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           >  SRR4302540.5587670/1‑94 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           >  SRR4302540.3995210/1‑94 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           <  SRR4302540.4155648/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTC                                           >  SRR4302540.3995209/1‑94 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGC                                          >  SRR4302540.4874922/1‑94 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGC                                          >  SRR4302540.8602091/1‑94 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGC                                          >  SRR4302540.8637962/1‑94 (MQ=60)
                                                  TCACCGTATTAACCGATGCGAAAAGGACCACAAGGGGGCGCAT‑GCGAGGCGCGCGTATGACACGCAAACACCGTCGCCACGCTCTTTCTGTGAC                                         >  SRR4302540.4829951/1‑94 (MQ=60)
                                                  TCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCC                                         >  SRR4302540.6813060/1‑94 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        <  SRR4302540.8514254/94‑1 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        <  SRR4302540.399127/94‑1 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        <  SRR4302540.785360/94‑1 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        >  SRR4302540.6680996/1‑94 (MQ=60)
                                                    ACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCG                                       <  SRR4302540.5972055/94‑1 (MQ=60)
                                                      CGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     >  SRR4302540.654197/1‑94 (MQ=60)
                                                      CGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     <  SRR4302540.2591523/94‑1 (MQ=60)
                                                      CGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     <  SRR4302540.3307335/94‑1 (MQ=60)
                                                       GTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGC                                    >  SRR4302540.2183558/1‑94 (MQ=60)
                                                        TATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCG                                   >  SRR4302540.1030731/1‑94 (MQ=60)
                                                         ATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGC                                  >  SRR4302540.3875915/1‑94 (MQ=60)
                                                         ATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAG‑ACGAGGCGCGCGTCTCACACTCAAACCCCATGGCCACGCCCTTTCTGTGCGCGTGCGC                                  >  SRR4302540.9671967/1‑94 (MQ=42)
                                                          TTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCA                                 >  SRR4302540.6630965/1‑94 (MQ=60)
                                                          TTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCA                                 <  SRR4302540.6695977/94‑1 (MQ=60)
                                                          TTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCA                                 >  SRR4302540.6223633/1‑94 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                <  SRR4302540.4796656/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302540.8633378/1‑94 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                <  SRR4302540.8402980/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                >  SRR4302540.1889367/1‑94 (MQ=60)
                                                            AACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGC                               >  SRR4302540.3204215/1‑94 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              <  SRR4302540.4840447/94‑1 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              <  SRR4302540.2461739/94‑1 (MQ=60)
                                                              CCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGG                             <  SRR4302540.3741212/94‑1 (MQ=60)
                                                              CCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGG                             <  SRR4302540.4512243/94‑1 (MQ=60)
                                                              CCGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGG                             <  SRR4302540.4744855/94‑1 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302540.3560784/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302540.2340618/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302540.4517786/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302540.3850056/1‑94 (MQ=60)
                                                                  TGCGAGAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCG                         <  SRR4302540.1464632/94‑1 (MQ=60)
                                                                   GCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAGCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG                        <  SRR4302540.9573400/94‑1 (MQ=60)
                                                                   GCGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG                        <  SRR4302540.895507/94‑1 (MQ=60)
                                                                    CGAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA                       >  SRR4302540.5110170/1‑94 (MQ=60)
                                                                     GAAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAA                      <  SRR4302540.6727819/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302540.450023/1‑94 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     <  SRR4302540.7344660/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     <  SRR4302540.5021947/94‑1 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCCCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     <  SRR4302540.1665585/94‑1 (MQ=60)
                                                                       AAAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGT                    <  SRR4302540.2068742/94‑1 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.10088763/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.8345158/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.1885232/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   <  SRR4302540.1913199/94‑1 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.4075838/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.5279223/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302540.8344894/1‑94 (MQ=60)
                                                                         AGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGG                  >  SRR4302540.7202794/1‑94 (MQ=60)
                                                                         AGGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGG                  >  SRR4302540.3189778/1‑94 (MQ=60)
                                                                          GGACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 >  SRR4302540.8785680/1‑94 (MQ=60)
                                                                          AGACAACAAGGCGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCAGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 <  SRR4302540.1950786/94‑1 (MQ=60)
                                                                            ACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCTACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGT               <  SRR4302540.5127042/94‑1 (MQ=60)
                                                                            ACAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGT               <  SRR4302540.8433288/94‑1 (MQ=60)
                                                                             CAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302540.19364/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTC              >  SRR4302540.8650232/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAAGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302540.879152/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             <  SRR4302540.9558321/94‑1 (MQ=60)
                                                                              AACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302540.3858921/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGAAGCGGCTCGGAAGTGGGGTTC             >  SRR4302540.9645381/1‑94 (MQ=60)
                                                                               ACAAGGGGGCGAAT‑GCGAGGCGCTCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCC            <  SRR4302540.7981768/94‑1 (MQ=60)
                                                                               ACAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAATCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCC            <  SRR4302540.5335904/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           <  SRR4302540.806464/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           <  SRR4302540.4890164/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           <  SRR4302540.6123460/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCACGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           <  SRR4302540.7238222/94‑1 (MQ=60)
                                                                                 AAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          >  SRR4302540.2718252/1‑94 (MQ=60)
                                                                                 AAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          <  SRR4302540.9873339/94‑1 (MQ=60)
                                                                                 AAGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGCTCCCG          >  SRR4302540.600170/1‑94 (MQ=60)
                                                                                  AGGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGC         >  SRR4302540.8940611/1‑94 (MQ=60)
                                                                                   GGGGGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCA        <  SRR4302540.9351029/94‑1 (MQ=60)
                                                                                      GGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302540.1338036/94‑1 (MQ=60)
                                                                                      GGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302540.3148919/94‑1 (MQ=60)
                                                                                      GGCGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302540.2425747/94‑1 (MQ=60)
                                                                                        CGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCC   >  SRR4302540.3393207/1‑94 (MQ=60)
                                                                                        CGAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCC   <  SRR4302540.5293881/94‑1 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302540.3461374/94‑1 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302540.2752847/94‑1 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  SRR4302540.9373536/1‑94 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302540.9212928/94‑1 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  SRR4302540.4955312/1‑94 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302540.7313563/94‑1 (MQ=60)
                                                                                         GAAT‑GCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCG  >  SRR4302540.2192800/1‑94 (MQ=60)
                                                                                              |                                                                                         
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAT‑CGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑