Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,403,945 T→C V131A (GTA→GCA)  accC → acetyl CoA carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,403,9450TC99.7% 1089.4 / ‑5.6 347V131A (GTA→GCA) accCacetyl CoA carboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (162/184);  total (162/185)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 359 total aligned reads displayed.

TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403855‑3404034
                                                                                          |                                                                                         
 cTTCATGGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGCCCCTTGCGCAcc                                                                                        <  2:7121716/93‑1 (MQ=255)
  ttgaTTGGCCCGCAGCCAGAACCCATTGGCCTGATGGGCGCCAAAGTATCCGCAATCGCGACGCTGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       <  1:1276572/90‑1 (MQ=255)
  ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       >  1:1592187/1‑93 (MQ=255)
   tCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCgg                                                                                      >  2:5827162/1‑93 (MQ=255)
   tCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCgg                                                                                      <  2:4027840/93‑1 (MQ=255)
    cATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCggg                                                                                     <  1:509339/93‑1 (MQ=255)
     aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  1:4723403/1‑93 (MQ=255)
     aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  1:7390497/1‑93 (MQ=255)
      ttGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCaggct                                                                                   >  2:4542784/1‑91 (MQ=255)
      ttGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGtt                                                                                   >  2:8888304/1‑93 (MQ=255)
          cccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGa                                                                               <  1:123229/93‑1 (MQ=255)
          cccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGa                                                                               <  1:8804973/93‑1 (MQ=255)
           ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              <  2:8218663/93‑1 (MQ=255)
            cGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACg                                                                             <  2:3104590/93‑1 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          <  1:2701126/93‑1 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          >  1:9037452/1‑93 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:5727653/93‑1 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  2:6123451/93‑1 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:1682249/93‑1 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:2843537/93‑1 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:3790278/93‑1 (MQ=255)
                    gAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTg                                                                     >  2:8078095/1‑93 (MQ=255)
                     aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCtcg                                                                    >  2:3635544/1‑91 (MQ=255)
                     aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    <  1:83949/93‑1 (MQ=255)
                     aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    >  1:4496180/1‑93 (MQ=255)
                         cATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcga                                                                >  1:4989253/1‑93 (MQ=255)
                          aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               <  1:5339619/93‑1 (MQ=255)
                              gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  1:4113434/93‑1 (MQ=255)
                               ccTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGatat                                                          <  1:8586265/93‑1 (MQ=255)
                                cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  2:1459375/93‑1 (MQ=255)
                                cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         >  1:2376468/1‑93 (MQ=255)
                                 tGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATgg                                                        <  1:9299010/93‑1 (MQ=255)
                                 tGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATgg                                                        >  2:9053085/1‑93 (MQ=255)
                                  gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       >  2:6382953/1‑93 (MQ=255)
                                  gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       >  1:6406832/1‑93 (MQ=255)
                                  gATCGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGTCGTCTCTTGCGCACCGGGTTCTGAAGGCCCTCTGGGCGGCGATATggt                                                       >  2:9840048/1‑92 (MQ=255)
                                   aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      <  2:8359383/93‑1 (MQ=255)
                                    tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  2:2819549/93‑1 (MQ=255)
                                    tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     >  1:696612/1‑93 (MQ=255)
                                    tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  1:9750284/93‑1 (MQ=255)
                                     ggggGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaa                                                    <  1:8626941/93‑1 (MQ=255)
                                     gggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaa                                                    >  2:5446273/1‑93 (MQ=255)
                                      ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   <  1:7899835/93‑1 (MQ=255)
                                      ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   <  2:459363/93‑1 (MQ=255)
                                      ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   >  1:5562799/1‑93 (MQ=255)
                                         gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                <  2:4380838/93‑1 (MQ=255)
                                         gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                <  2:5290534/93‑1 (MQ=255)
                                         gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                <  2:2653024/93‑1 (MQ=255)
                                         gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                <  2:1310645/93‑1 (MQ=255)
                                          aCAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAcc                                               >  2:8614460/1‑93 (MQ=255)
                                           cAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCg                                              >  2:6288945/1‑93 (MQ=255)
                                            aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             <  1:8901954/93‑1 (MQ=255)
                                             aaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTg                                            >  1:7189881/1‑93 (MQ=255)
                                              aGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGc                                           <  1:10010924/93‑1 (MQ=255)
                                               gTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGcc                                          >  2:9506202/1‑93 (MQ=255)
                                                tctCCGCAATCGCGGCGATGAAAAAAGCGGGCGACCCTTGCACACCGGGTTCTAACGGCCCGCTGGGCGACGATATGGATAAAAACCTTGCCa                                         <  2:6144275/91‑1 (MQ=255)
                                                 aTCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGATGATATGGATAAAAACCGTGCCAt                                        <  1:4773262/93‑1 (MQ=255)
                                                 aTCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAt                                        <  2:7626741/93‑1 (MQ=255)
                                                 aTCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGAGACGATATGGATAAAAACCGTGCCAt                                        <  1:3223759/93‑1 (MQ=255)
                                                  tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  2:7437279/93‑1 (MQ=255)
                                                  tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       >  1:5629675/1‑93 (MQ=255)
                                                     gCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCt                                    >  1:8756665/1‑93 (MQ=255)
                                                      cAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTa                                   >  1:9111384/1‑93 (MQ=255)
                                                         tCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAc                                >  1:2060571/1‑93 (MQ=255)
                                                         tCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAc                                <  2:5955996/93‑1 (MQ=255)
                                                           gcggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGc                              >  1:6642646/1‑93 (MQ=255)
                                                           gcggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGc                              >  1:3021442/1‑93 (MQ=255)
                                                             ggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  1:9979129/93‑1 (MQ=255)
                                                              gcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAtt                           >  1:7543023/1‑93 (MQ=255)
                                                               ggATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          <  1:5938294/92‑1 (MQ=255)
                                                               cgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          >  2:9275056/1‑93 (MQ=255)
                                                               cgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          <  2:6452041/93‑1 (MQ=255)
                                                                gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         >  2:6595628/1‑93 (MQ=255)
                                                                gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         >  1:7611691/1‑93 (MQ=255)
                                                                 aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        >  1:9698755/1‑93 (MQ=255)
                                                                  tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGACATTGCTAAAAGCATTGGtt                       >  2:5201327/1‑93 (MQ=255)
                                                                   gAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTa                      <  1:7756759/93‑1 (MQ=255)
                                                                     aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    <  1:1395397/93‑1 (MQ=255)
                                                                     aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    <  1:8173224/93‑1 (MQ=255)
                                                                     aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    >  2:1761752/1‑93 (MQ=255)
                                                                      aaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATcc                   <  2:2090093/93‑1 (MQ=255)
                                                                      aaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATcc                   >  2:6802315/1‑93 (MQ=255)
                                                                         aGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGt                <  1:5405377/93‑1 (MQ=255)
                                                                           cGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGa              <  1:9938559/93‑1 (MQ=255)
                                                                           cGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGa              <  2:34168/93‑1 (MQ=255)
                                                                            gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             >  2:5642226/1‑93 (MQ=255)
                                                                            gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             <  2:285014/93‑1 (MQ=255)
                                                                            gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             >  2:1141267/1‑93 (MQ=255)
                                                                              gCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTa           <  1:1524060/93‑1 (MQ=255)
                                                                                 tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  1:4167874/1‑93 (MQ=255)
                                                                                 tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  1:6121403/1‑93 (MQ=255)
                                                                                  cccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaa       <  2:7843429/93‑1 (MQ=255)
                                                                                  cccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaa       >  2:3779409/1‑93 (MQ=255)
                                                                                   ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      >  1:5825426/1‑93 (MQ=255)
                                                                                   ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      <  2:2497446/93‑1 (MQ=255)
                                                                                     ttGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGc    >  2:8565123/1‑93 (MQ=255)
                                                                                     ttGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGc    >  2:9180523/1‑93 (MQ=255)
                                                                                      tGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGcc   >  2:4931610/1‑93 (MQ=255)
                                                                                       gcgcACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCt  <  1:8338388/93‑1 (MQ=255)
                                                                                       gcgcACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCt  >  2:3478743/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403855‑3404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 205 total aligned reads displayed.
TATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  NC_000913/3403853‑3404038
                                                                                            |                                                                                             
TATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCA                                                                                              >  SRR4302540.7019841/1‑94 (MQ=60)
 TTCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             <  SRR4302540.9134106/94‑1 (MQ=60)
 ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             <  SRR4302540.6708382/94‑1 (MQ=60)
 ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             <  SRR4302540.3346431/94‑1 (MQ=60)
 ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             <  SRR4302540.7757603/94‑1 (MQ=60)
  TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACC                                                                                            >  SRR4302540.4462317/1‑94 (MQ=60)
  TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACC                                                                                            >  SRR4302540.2768058/1‑94 (MQ=60)
   CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302540.5098504/1‑94 (MQ=60)
   CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302540.1609582/1‑94 (MQ=60)
   CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302540.1609580/1‑94 (MQ=60)
    TTGATTGGCCCGCAGCCAGAACCCATTGGCCTGATGGGCGCCAAAGTATCCGCAATCGCGACGCTGAAAAAAGCGGGCGTCCCTTGCGCACCGG                                                                                          <  SRR4302540.1289733/94‑1 (MQ=50)
      CATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGT                                                                                        >  SRR4302540.7472842/1‑94 (MQ=60)
      CATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGT                                                                                        >  SRR4302540.4773854/1‑94 (MQ=60)
      CATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGT                                                                                        <  SRR4302540.514804/94‑1 (MQ=60)
       ATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTT                                                                                       <  SRR4302540.4627923/94‑1 (MQ=60)
       ATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTT                                                                                       >  SRR4302540.7914772/1‑94 (MQ=60)
        TTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTC                                                                                      >  SRR4302540.9154490/1‑94 (MQ=60)
        TTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTC                                                                                      >  SRR4302540.9154491/1‑94 (MQ=60)
         TGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCT                                                                                     <  SRR4302540.4943720/94‑1 (MQ=60)
         TGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCT                                                                                     <  SRR4302540.5938019/94‑1 (MQ=60)
         TGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCT                                                                                     <  SRR4302540.8390715/94‑1 (MQ=60)
         TGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCT                                                                                     >  SRR4302540.5879985/1‑94 (MQ=60)
            CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAGGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                                  <  SRR4302540.8237003/94‑1 (MQ=60)
            CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                                  <  SRR4302540.9265308/94‑1 (MQ=60)
            CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                                  <  SRR4302540.124542/94‑1 (MQ=60)
            CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                                  <  SRR4302540.8906759/94‑1 (MQ=60)
             CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                                 <  SRR4302540.1258564/94‑1 (MQ=60)
              CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGG                                                                                >  SRR4302540.9530235/1‑94 (MQ=60)
              CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGG                                                                                >  SRR4302540.906683/1‑94 (MQ=60)
               GAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGC                                                                               >  SRR4302540.2247475/1‑94 (MQ=60)
                AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                              >  SRR4302540.9145929/1‑94 (MQ=60)
                 AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCC                                                                             <  SRR4302540.2730274/94‑1 (MQ=60)
                 AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCC                                                                             <  SRR4302540.1445986/94‑1 (MQ=60)
                  AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGCTCTGACGGCCCG                                                                            >  SRR4302540.5206702/1‑94 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.5790727/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          >  SRR4302540.7833426/1‑94 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.2874307/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.1700511/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.8590885/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.3831601/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          >  SRR4302540.1464144/1‑94 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          >  SRR4302540.1464143/1‑94 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302540.5790726/94‑1 (MQ=60)
                    CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCG                                                                          >  SRR4302540.899619/1‑94 (MQ=60)
                     AGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTG                                                                         <  SRR4302540.3894822/94‑1 (MQ=60)
                     AGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGCGTTCTGACGGCCCGCTG                                                                         >  SRR4302540.1671624/1‑94 (MQ=60)
                      GAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGG                                                                        >  SRR4302540.4544927/1‑94 (MQ=60)
                        AGCCATCCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCGCCGGGTTCTGACGGCCCGCTGGGC                                                                      <  SRR4302540.3683493/94‑1 (MQ=60)
                        AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                      <  SRR4302540.8424220/94‑1 (MQ=60)
                        AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGCTTCTGACGGCCCGCTGGGC                                                                      >  SRR4302540.2943830/1‑94 (MQ=60)
                        AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGCCGCCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                      <  SRR4302540.2114800/94‑1 (MQ=60)
                         ACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCG                                                                     <  SRR4302540.9017253/94‑1 (MQ=60)
                           CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   <  SRR4302540.4559681/94‑1 (MQ=60)
                           CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   >  SRR4302540.5470480/1‑94 (MQ=60)
                           CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   <  SRR4302540.8202024/94‑1 (MQ=60)
                            ctgcgccgaattcgcaaaaaacaatcccgaatccccGCGATGAAAAAATCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302540.2412360/58‑1 (MQ=60)
                            ATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302540.5398162/94‑1 (MQ=60)
                            ATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302540.5630784/94‑1 (MQ=60)
                            ATCCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302540.2412358/94‑1 (MQ=60)
                              TCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGAT                                                                <  SRR4302540.9649702/94‑1 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302540.8861157/94‑1 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              >  SRR4302540.8510980/1‑94 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302540.4157845/94‑1 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302540.7533988/94‑1 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302540.1305144/94‑1 (MQ=60)
                                GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302540.4157846/94‑1 (MQ=60)
                                 CCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATG                                                             <  SRR4302540.8686079/94‑1 (MQ=60)
                                 CCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATG                                                             >  SRR4302540.2402081/1‑94 (MQ=60)
                                  CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            >  SRR4302540.324687/1‑94 (MQ=60)
                                  CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            <  SRR4302540.10102846/94‑1 (MQ=60)
                                  CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            >  SRR4302540.324686/1‑94 (MQ=60)
                                  CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            >  SRR4302540.7036685/1‑94 (MQ=60)
                                   TGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGA                                                           >  SRR4302540.6478143/1‑94 (MQ=60)
                                   TGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGA                                                           <  SRR4302540.9410096/94‑1 (MQ=60)
                                   TGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGA                                                           <  SRR4302540.6385860/94‑1 (MQ=60)
                                   TGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGA                                                           >  SRR4302540.7431679/1‑94 (MQ=60)
                                   TGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGA                                                           >  SRR4302540.875146/1‑94 (MQ=60)
                                     ATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATA                                                         >  SRR4302540.704088/1‑94 (MQ=60)
                                      TGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAA                                                        <  SRR4302540.9864696/94‑1 (MQ=60)
                                      TGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAA                                                        <  SRR4302540.2081333/94‑1 (MQ=60)
                                      TGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAA                                                        <  SRR4302540.5294754/94‑1 (MQ=60)
                                       GGGGGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAA                                                       <  SRR4302540.8727152/94‑1 (MQ=60)
                                       GGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAA                                                       <  SRR4302540.8727150/94‑1 (MQ=60)
                                       GGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAA                                                       >  SRR4302540.5624057/1‑94 (MQ=60)
                                       GGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAA                                                       <  SRR4302540.8727151/94‑1 (MQ=60)
                                        GGCGACAAAGTATCCGCAATCGCGGCGATGAAATAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAA                                                      <  SRR4302540.9841852/94‑1 (MQ=60)
                                        GGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAA                                                      <  SRR4302540.7991982/94‑1 (MQ=60)
                                        GGCAACAAAGTATCCGCAATCGCGCCGATGAAAGAAGCGGGCGTCCATTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAA                                                      <  SRR4302540.9841851/94‑1 (MQ=60)
                                          CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                    >  SRR4302540.1063570/1‑94 (MQ=60)
                                          CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                    >  SRR4302540.8941491/1‑94 (MQ=60)
                                          CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                    <  SRR4302540.887239/94‑1 (MQ=60)
                                           GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACC                                                   >  SRR4302540.1166772/1‑94 (MQ=60)
                                           GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACC                                                   <  SRR4302540.1559009/94‑1 (MQ=60)
                                           GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACC                                                   <  SRR4302540.4625904/94‑1 (MQ=60)
                                           GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACC                                                   >  SRR4302540.1625213/1‑94 (MQ=60)
                                           GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAAC                                                   >  SRR4302540.6592824/1‑94 (MQ=60)
                                            ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAACACCG                                                  >  SRR4302540.7089653/1‑94 (MQ=60)
                                            ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  <  SRR4302540.7684211/94‑1 (MQ=60)
                                            ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  >  SRR4302540.7089654/1‑94 (MQ=60)
                                            ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  <  SRR4302540.200116/94‑1 (MQ=60)
                                            ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  <  SRR4302540.6264932/94‑1 (MQ=60)
                                             CAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGT                                                 >  SRR4302540.7571765/1‑94 (MQ=60)
                                              AAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTG                                                >  SRR4302540.7270022/1‑94 (MQ=60)
                                              AAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTG                                                <  SRR4302540.1357524/94‑1 (MQ=60)
                                              AAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTG                                                <  SRR4302540.9005004/94‑1 (MQ=60)
                                               AAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGC                                               >  SRR4302540.1580498/1‑94 (MQ=60)
                                               AAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGC                                               >  SRR4302540.7510113/1‑94 (MQ=60)
                                                AGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCC                                              <  SRR4302540.10127491/94‑1 (MQ=60)
                                                AGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCC                                              <  SRR4302540.10066587/94‑1 (MQ=60)
                                                  TATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAT                                            <  SRR4302540.562445/94‑1 (MQ=60)
                                                  TATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAT                                            <  SRR4302540.562444/94‑1 (MQ=60)
                                                  TATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAT                                            <  SRR4302540.5138560/94‑1 (MQ=60)
                                                  TATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAT                                            <  SRR4302540.6057584/94‑1 (MQ=60)
                                                  TATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAT                                            <  SRR4302540.9333082/94‑1 (MQ=60)
                                                   ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGATGATATGGATAAAAACCGTGCCATT                                           <  SRR4302540.4824254/94‑1 (MQ=60)
                                                   ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                           >  SRR4302540.5691637/1‑94 (MQ=60)
                                                   ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                           >  SRR4302540.8826932/1‑94 (MQ=60)
                                                   ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGAGACGATATGGATAAAAACCGTGCCATT                                           <  SRR4302540.3258802/94‑1 (MQ=60)
                                                    TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                          <  SRR4302540.5724919/94‑1 (MQ=60)
                                                    TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                          >  SRR4302540.532261/1‑94 (MQ=60)
                                                     CCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGC                                         >  SRR4302540.4966807/1‑94 (MQ=60)
                                                     CCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCATGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGC                                         <  SRR4302540.9059273/94‑1 (MQ=60)
                                                      CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                        >  SRR4302540.8858231/1‑94 (MQ=60)
                                                      CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                        >  SRR4302540.1279836/1‑94 (MQ=60)
                                                      CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                        >  SRR4302540.1279840/1‑94 (MQ=60)
                                                      CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                        >  SRR4302540.2960722/1‑94 (MQ=60)
                                                       GCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTA                                       <  SRR4302540.1003103/94‑1 (MQ=60)
                                                       GCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTA                                       >  SRR4302540.9220888/1‑94 (MQ=60)
                                                       GCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTA                                       >  SRR4302540.7331967/1‑94 (MQ=60)
                                                        CAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAA                                      <  SRR4302540.3741069/94‑1 (MQ=60)
                                                         AATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAA                                     >  SRR4302540.5458006/1‑94 (MQ=60)
                                                          ATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAC                                    >  SRR4302540.2082747/1‑94 (MQ=60)
                                                           TCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACG                                   >  SRR4302540.8670349/1‑94 (MQ=60)
                                                            CGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGC                                  >  SRR4302540.6716142/1‑94 (MQ=60)
                                                            CGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGC                                  >  SRR4302540.3054649/1‑94 (MQ=60)
                                                              CGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAT                                >  SRR4302540.3049706/1‑94 (MQ=60)
                                                              CGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAT                                <  SRR4302540.3226096/94‑1 (MQ=60)
                                                              CGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAT                                <  SRR4302540.5451523/94‑1 (MQ=60)
                                                               GGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATT                               >  SRR4302540.7627541/1‑94 (MQ=60)
                                                               GGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATT                               <  SRR4302540.10095490/94‑1 (MQ=60)
                                                               GGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATT                               >  SRR4302540.1934652/1‑94 (MQ=60)
                                                                 GGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGG                             <  SRR4302540.6003937/94‑1 (MQ=60)
                                                                 CGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGG                             >  SRR4302540.7697241/1‑94 (MQ=60)
                                                                 CGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGG                             >  SRR4302540.7697242/1‑94 (MQ=60)
                                                                  GATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGT                            >  SRR4302540.9812784/1‑94 (MQ=60)
                                                                  GATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGT                            >  SRR4302540.9812785/1‑94 (MQ=60)
                                                                  GATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGT                            <  SRR4302540.9379506/94‑1 (MQ=60)
                                                                   ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                           <  SRR4302540.9855583/94‑1 (MQ=60)
                                                                     GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                         <  SRR4302540.6030000/94‑1 (MQ=60)
                                                                     GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                         <  SRR4302540.7847392/94‑1 (MQ=60)
                                                                      AAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATC                        >  SRR4302540.2760334/1‑94 (MQ=60)
                                                                       AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                       >  SRR4302540.7802186/1‑94 (MQ=60)
                                                                       AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                       <  SRR4302540.8268162/94‑1 (MQ=60)
                                                                       AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                       <  SRR4302540.1410359/94‑1 (MQ=60)
                                                                        AAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                      <  SRR4302540.481119/94‑1 (MQ=60)
                                                                           AGCGGGCGTCCCTTTCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTG                   <  SRR4302540.8122709/94‑1 (MQ=60)
                                                                           AGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTG                   <  SRR4302540.5464808/94‑1 (MQ=60)
                                                                             CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 <  SRR4302540.10054574/94‑1 (MQ=60)
                                                                             CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 <  SRR4302540.255341/94‑1 (MQ=60)
                                                                             CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 <  SRR4302540.5467148/94‑1 (MQ=60)
                                                                             CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 <  SRR4302540.5467147/94‑1 (MQ=60)
                                                                              GGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATT                >  SRR4302540.581533/1‑94 (MQ=60)
                                                                              GGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATT                <  SRR4302540.7967959/94‑1 (MQ=60)
                                                                                GCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAT              <  SRR4302540.1540928/94‑1 (MQ=60)
                                                                                 CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATC             <  SRR4302540.4844659/94‑1 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.8913172/1‑94 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.6189581/1‑94 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.4213069/1‑94 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.7006322/1‑94 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            <  SRR4302540.7276273/94‑1 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            <  SRR4302540.4171056/94‑1 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.3871783/1‑94 (MQ=60)
                                                                                  GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA            >  SRR4302540.3421452/1‑94 (MQ=60)
                                                                                   TCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAA           >  SRR4302540.4815439/1‑94 (MQ=60)
                                                                                    CCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAA          <  SRR4302540.9974816/94‑1 (MQ=60)
                                                                                    CCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAA          >  SRR4302540.5889825/1‑94 (MQ=60)
                                                                                    CCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAA          >  SRR4302540.5969661/1‑94 (MQ=60)
                                                                                    CCCTTGCGCACCGGGTTCCGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGTATTGGTTATCCGGTGATTATCAAA          >  SRR4302540.5889824/1‑94 (MQ=60)
                                                                                     CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302540.8437311/94‑1 (MQ=60)
                                                                                     CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302540.10076652/94‑1 (MQ=60)
                                                                                     CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302540.8437312/94‑1 (MQ=60)
                                                                                     CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302540.4517095/94‑1 (MQ=60)
                                                                                     CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302540.6200088/94‑1 (MQ=60)
                                                                                      CTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATACAAGC        >  SRR4302540.7766191/1‑94 (MQ=60)
                                                                                       TTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCC       <  SRR4302540.6017446/94‑1 (MQ=60)
                                                                                       TTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCC       <  SRR4302540.4381997/94‑1 (MQ=60)
                                                                                        TGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT      <  SRR4302540.6759726/94‑1 (MQ=60)
                                                                                        TGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT      <  SRR4302540.8732865/94‑1 (MQ=60)
                                                                                        TGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT      >  SRR4302540.4056627/1‑94 (MQ=60)
                                                                                         GCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTC     <  SRR4302540.8435168/94‑1 (MQ=60)
                                                                                         GCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTC     <  SRR4302540.8435173/94‑1 (MQ=60)
                                                                                          CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    <  SRR4302540.8137809/94‑1 (MQ=60)
                                                                                          CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    <  SRR4302540.327729/94‑1 (MQ=60)
                                                                                          CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    <  SRR4302540.2099613/94‑1 (MQ=60)
                                                                                          CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    <  SRR4302540.9962264/94‑1 (MQ=60)
                                                                                           GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAGCCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302540.5612263/94‑1 (MQ=60)
                                                                                           GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   >  SRR4302540.5362767/1‑94 (MQ=60)
                                                                                           GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302540.2309674/94‑1 (MQ=60)
                                                                                           GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   >  SRR4302540.5362769/1‑94 (MQ=60)
                                                                                            CACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  SRR4302540.4549234/1‑94 (MQ=60)
                                                                                            |                                                                                             
TATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  NC_000913/3403853‑3404038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑