Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,454,538 T→C N175N (AAT→AAC)  b3325 → YheF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,454,5380TC99.8% 1465.0 / ‑5.2 444N175N (AAT→AAC) b3325YheF
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (236/206);  total (237/207)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 462 total aligned reads displayed.

CCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454448‑3454627
                                                                                          |                                                                                         
ccAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATtaacaa                                                                                         <  1:605940/93‑1 (MQ=255)
 cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        <  1:5193817/93‑1 (MQ=255)
  aGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAc                                                                                       <  1:9048511/93‑1 (MQ=255)
  aGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAc                                                                                       <  1:7074436/93‑1 (MQ=255)
     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:5793575/1‑93 (MQ=255)
     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:6516179/1‑93 (MQ=255)
     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:8489377/1‑93 (MQ=255)
           atgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAg                                                                              <  2:9775279/93‑1 (MQ=255)
            tgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGt                                                                             <  2:1097047/93‑1 (MQ=255)
              ggggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCa                                                                           <  2:8897158/92‑1 (MQ=255)
               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACACACTGATTGAAGTCAt                                                                          >  2:4409769/1‑93 (MQ=255)
               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          >  2:8572037/1‑93 (MQ=255)
               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          >  2:8415415/1‑93 (MQ=255)
                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  1:1767252/1‑93 (MQ=255)
                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  1:5366577/1‑93 (MQ=255)
                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  2:6199224/1‑93 (MQ=255)
                 gTATCGTCGGTAATGTTGTGCATTATGAACCCTCCCACGTTCTTATTCTGCCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  1:1301232/93‑1 (MQ=255)
                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:8261009/93‑1 (MQ=255)
                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:8707814/93‑1 (MQ=255)
                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:9167528/93‑1 (MQ=255)
                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:5406570/93‑1 (MQ=255)
                  gaGCGTCGGTAATATTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaaa                                                                       <  1:8293848/92‑1 (MQ=255)
                   aGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAg                                                                      <  1:2354524/93‑1 (MQ=255)
                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     >  2:6521877/1‑93 (MQ=255)
                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     >  2:3109832/1‑93 (MQ=255)
                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     <  1:2175900/93‑1 (MQ=255)
                      gTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgcgc                                                                   <  2:5317625/93‑1 (MQ=255)
                       tCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACANANTGATTGAAGTCATAAAGcgcg                                                                  >  1:1866859/1‑93 (MQ=255)
                        cGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGt                                                                 >  2:3403674/1‑93 (MQ=255)
                         ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                <  2:7626986/93‑1 (MQ=255)
                          gTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTg                                                               <  2:2949724/93‑1 (MQ=255)
                           tAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGa                                                              <  1:8774453/93‑1 (MQ=255)
                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  1:1204837/1‑93 (MQ=255)
                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  2:8137197/1‑93 (MQ=255)
                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  2:6100563/1‑93 (MQ=255)
                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             <  2:878617/93‑1 (MQ=255)
                             aTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATg                                                            >  1:10039753/1‑93 (MQ=255)
                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           <  2:4226650/93‑1 (MQ=255)
                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  2:355169/1‑93 (MQ=255)
                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:2472004/1‑93 (MQ=255)
                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:8093479/1‑93 (MQ=255)
                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:4117976/1‑93 (MQ=255)
                               gttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTc                                                          >  1:8953812/1‑93 (MQ=255)
                                ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCa                                                         >  2:9355125/1‑93 (MQ=255)
                                ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCa                                                         <  1:4813865/93‑1 (MQ=255)
                                   tgCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCg                                                      <  1:6432145/93‑1 (MQ=255)
                                       ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAGGTCATAAAGCGCGTTGATGTCATCGGcac                                                  <  1:6364113/93‑1 (MQ=255)
                                       ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCATGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcac                                                  >  1:1965225/1‑93 (MQ=255)
                                        tATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcaca                                                 >  1:364253/1‑93 (MQ=255)
                                         aTGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACag                                                <  2:184289/93‑1 (MQ=255)
                                           gAACCCTCCAACGTTCTTTTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACagag                                              >  1:5066519/1‑93 (MQ=255)
                                           gAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACagag                                              >  2:2262852/1‑93 (MQ=255)
                                            aaCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             <  2:211428/93‑1 (MQ=255)
                                             aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGaa                                            <  1:5608555/93‑1 (MQ=255)
                                              cccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAag                                           <  2:2546290/93‑1 (MQ=255)
                                               ccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAaga                                          >  2:7398231/1‑92 (MQ=255)
                                                    aaCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaa                                     >  2:1542517/1‑93 (MQ=255)
                                                    aaCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaa                                     >  2:2181980/1‑93 (MQ=255)
                                                     aCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaaa                                    <  1:9408899/93‑1 (MQ=255)
                                                     aCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaaa                                    <  1:1628707/93‑1 (MQ=255)
                                                      cGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAat                                   <  2:7792796/93‑1 (MQ=255)
                                                       gTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatt                                  >  1:3167306/1‑93 (MQ=255)
                                                       gTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatt                                  >  1:7631721/1‑93 (MQ=255)
                                                        ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 <  1:1057633/93‑1 (MQ=255)
                                                        ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 >  1:6760108/1‑93 (MQ=255)
                                                         tCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAattat                                <  2:6996984/93‑1 (MQ=255)
                                                         tCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGCCACAGAGAAGCAGCACattat                                >  2:454904/1‑93 (MQ=255)
                                                          cTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAattatt                               >  1:9963236/1‑93 (MQ=255)
                                                            tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCa                             <  1:7374399/93‑1 (MQ=255)
                                                            tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATCTCATCGGCACAGAGAAGCAGCAAATTATTCa                             >  2:1891832/1‑93 (MQ=255)
                                                             aTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAt                            >  2:5708173/1‑93 (MQ=255)
                                                              ttCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATc                           <  2:5201237/93‑1 (MQ=255)
                                                              ttCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATc                           >  2:9594444/1‑93 (MQ=255)
                                                               tCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCt                          >  1:9727580/1‑93 (MQ=255)
                                                               tCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGGCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCt                          <  1:7884419/93‑1 (MQ=255)
                                                                 tGACGGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTgg                        <  2:3975519/93‑1 (MQ=255)
                                                                 tGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTgg                        >  2:7957824/1‑93 (MQ=255)
                                                                   aCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGaa                      <  2:7055260/93‑1 (MQ=255)
                                                                    ccGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAat                     >  2:4801137/1‑93 (MQ=255)
                                                                       gTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATg                  >  1:7158000/1‑93 (MQ=255)
                                                                       gTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATg                  >  2:3887773/1‑93 (MQ=255)
                                                                        tCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGc                 <  2:2422930/93‑1 (MQ=255)
                                                                          gTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGt               <  1:5457833/93‑1 (MQ=255)
                                                                           tGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTc              <  2:2008707/93‑1 (MQ=255)
                                                                             cctcctCCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAg            <  2:4843950/93‑1 (MQ=255)
                                                                               tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  2:1415358/1‑93 (MQ=255)
                                                                               tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  1:2836115/1‑93 (MQ=255)
                                                                                ccaccaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCgg         >  2:8458723/1‑93 (MQ=255)
                                                                                 caccaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGa        >  1:1504705/1‑93 (MQ=255)
                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  2:5886350/1‑93 (MQ=255)
                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  2:2327391/1‑93 (MQ=255)
                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  1:2642349/1‑93 (MQ=255)
                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  1:3846909/1‑93 (MQ=255)
                                                                                    caTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGa     <  2:4984582/93‑1 (MQ=255)
                                                                                     aTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAt    <  1:6167221/93‑1 (MQ=255)
                                                                                     aTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAt    >  1:3458740/1‑93 (MQ=255)
                                                                                      ttAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGagc   >  1:8637750/1‑91 (MQ=255)
                                                                                      ttAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtc   >  1:4222531/1‑93 (MQ=255)
                                                                                       taacaaACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtct  <  1:5739774/93‑1 (MQ=255)
                                                                                       taacaaACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtct  <  2:6697957/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454448‑3454627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.
CCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCC  >  NC_000913/3454445‑3454631
                                                                                             |                                                                                             
CCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAAC                                                                                               >  SRR4302540.8118265/1‑94 (MQ=60)
CCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAAC                                                                                               >  SRR4302540.8092415/1‑94 (MQ=60)
  GCCAGAAGATGGATGCGGGTAGCGTCGGTATTGTTGTGCATTATGAACCCTCTAACGTTCCTATTTTGACTGGTCGGGCCTCCACCATTAACAA                                                                                             >  SRR4302540.9095918/1‑94 (MQ=60)
  CCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAA                                                                                             <  SRR4302540.9022379/94‑1 (MQ=60)
   CCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAA                                                                                            <  SRR4302540.612420/94‑1 (MQ=60)
    CAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAC                                                                                           <  SRR4302540.5250376/94‑1 (MQ=60)
    CAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAC                                                                                           >  SRR4302540.6537705/1‑94 (MQ=60)
     AGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTCACAAACT                                                                                          >  SRR4302540.7200435/1‑94 (MQ=60)
     AGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACT                                                                                          <  SRR4302540.5086316/94‑1 (MQ=60)
     AGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACT                                                                                          <  SRR4302540.9157135/94‑1 (MQ=60)
     AGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACT                                                                                          <  SRR4302540.8761528/94‑1 (MQ=60)
      GATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTG                                                                                         >  SRR4302540.5918955/1‑94 (MQ=60)
       ATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATTAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGA                                                                                        >  SRR4302540.582795/1‑94 (MQ=60)
       ATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGT                                                                                        >  SRR4302540.8138519/1‑94 (MQ=60)
       ATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGA                                                                                        >  SRR4302540.6588571/1‑94 (MQ=60)
       ATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGA                                                                                        >  SRR4302540.8588018/1‑94 (MQ=60)
       ATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGA                                                                                        >  SRR4302540.842676/1‑94 (MQ=60)
        TGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGAT                                                                                       >  SRR4302540.7589138/1‑94 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      >  SRR4302540.5684798/1‑94 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      <  SRR4302540.2029728/94‑1 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      <  SRR4302540.6133107/94‑1 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      >  SRR4302540.5582049/1‑94 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      >  SRR4302540.5582050/1‑94 (MQ=60)
         GATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATT                                                                                      >  SRR4302540.952436/1‑94 (MQ=60)
            GGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAA                                                                                   <  SRR4302540.7811321/94‑1 (MQ=60)
             GATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAG                                                                                  >  SRR4302540.3812315/1‑94 (MQ=60)
             GATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAG                                                                                  <  SRR4302540.5296114/94‑1 (MQ=60)
             GATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAG                                                                                  <  SRR4302540.4261645/94‑1 (MQ=60)
             GATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCACTAACAAACTGCTTGAAG                                                                                  >  SRR4302540.3812020/1‑94 (MQ=60)
             GATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAAACCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAA                                                                                  >  SRR4302540.8973553/1‑94 (MQ=60)
              ATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGT                                                                                 >  SRR4302540.1685053/1‑94 (MQ=60)
               TGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTC                                                                                <  SRR4302540.1929519/94‑1 (MQ=60)
                GCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCA                                                                               <  SRR4302540.1629373/94‑1 (MQ=60)
                 CGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAT                                                                              >  SRR4302540.3246874/1‑94 (MQ=60)
                  GGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATA                                                                             <  SRR4302540.7241724/94‑1 (MQ=60)
                  GGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATA                                                                             >  SRR4302540.5425542/1‑94 (MQ=60)
                   GGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAA                                                                            >  SRR4302540.6613626/1‑94 (MQ=60)
                    GTATCGTCGGTAATGTTGTGCATTATGAACCCTCCCACGTTCTTATTCTGCCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAA                                                                           <  SRR4302540.1314721/94‑1 (MQ=60)
                    GTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAA                                                                           >  SRR4302540.7587040/1‑94 (MQ=60)
                     TAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAG                                                                          <  SRR4302540.7897074/94‑1 (MQ=60)
                     TAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATAGAAGACATAAAG                                                                          >  SRR4302540.9060700/1‑94 (MQ=60)
                     GAGCGTCGGTAATATTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAG                                                                          <  SRR4302540.8390159/94‑1 (MQ=60)
                      AGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGC                                                                         >  SRR4302540.2893226/1‑94 (MQ=60)
                      AGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGC                                                                         >  SRR4302540.6385141/1‑94 (MQ=60)
                      AGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGC                                                                         <  SRR4302540.2379884/94‑1 (MQ=60)
                       GCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCG                                                                        <  SRR4302540.2199366/94‑1 (MQ=60)
                       GCCTCGGTAATGTTGGGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCG                                                                        <  SRR4302540.2223047/94‑1 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCGAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCT                                                                      >  SRR4302540.3804804/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACANANTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.1887314/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.5344222/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.33649/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.26247/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.1853058/1‑94 (MQ=60)
                         GTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCG                                                                      >  SRR4302540.6493705/1‑94 (MQ=60)
                           CGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTT                                                                    >  SRR4302540.9703280/1‑94 (MQ=60)
                            GGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTG                                                                   >  SRR4302540.4898118/1‑94 (MQ=60)
                            GGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTG                                                                   <  SRR4302540.5241395/94‑1 (MQ=60)
                            GGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTG                                                                   >  SRR4302540.6996604/1‑94 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.197123/94‑1 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.5560534/94‑1 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.6469096/94‑1 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.2032834/94‑1 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 >  SRR4302540.1217207/1‑94 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.8876101/94‑1 (MQ=60)
                              TAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAT                                                                 <  SRR4302540.1139831/94‑1 (MQ=60)
                               AATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATG                                                                >  SRR4302540.10156492/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.4190556/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.8006852/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.4162436/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.6438975/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.1225794/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.2498617/1‑94 (MQ=60)
                                ATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGT                                                               >  SRR4302540.2498616/1‑94 (MQ=60)
                                 TGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTC                                                              >  SRR4302540.9059471/1‑94 (MQ=60)
                                 TGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTC                                                              <  SRR4302540.5842502/94‑1 (MQ=60)
                                 TGTTGTGCAGTATGACCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTC                                                              <  SRR4302540.5067671/94‑1 (MQ=60)
                                  GTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCA                                                             <  SRR4302540.3408427/94‑1 (MQ=60)
                                  GTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCA                                                             <  SRR4302540.1656927/94‑1 (MQ=60)
                                   TTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCAT                                                            <  SRR4302540.6904539/94‑1 (MQ=60)
                                   TTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCAT                                                            >  SRR4302540.7111930/1‑94 (MQ=60)
                                   TTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCAT                                                            <  SRR4302540.4865390/94‑1 (MQ=60)
                                    TGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATC                                                           >  SRR4302540.5469548/1‑94 (MQ=60)
                                    TGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATC                                                           <  SRR4302540.6267719/94‑1 (MQ=60)
                                     GTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCG                                                          <  SRR4302540.3762913/94‑1 (MQ=60)
                                     GTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCG                                                          <  SRR4302540.856254/94‑1 (MQ=60)
                                      TGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGG                                                         >  SRR4302540.9706073/1‑94 (MQ=60)
                                      TGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGG                                                         <  SRR4302540.1568997/94‑1 (MQ=60)
                                      TGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGG                                                         <  SRR4302540.6503737/94‑1 (MQ=60)
                                      TGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGG                                                         <  SRR4302540.8236613/94‑1 (MQ=60)
                                       GCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGC                                                        >  SRR4302540.10038550/1‑94 (MQ=60)
                                       GCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATAGAAGTCATAAAGCGCGTTGATGTCATCGGC                                                        <  SRR4302540.1423523/94‑1 (MQ=60)
                                         ATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCAC                                                      >  SRR4302540.1065781/1‑94 (MQ=60)
                                         ATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCAC                                                      >  SRR4302540.6293416/1‑94 (MQ=60)
                                         ATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCATGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCAC                                                      >  SRR4302540.1986569/1‑94 (MQ=60)
                                          TTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAGGTCATAAAGCGCGTTGATGTCATCGGCACA                                                     <  SRR4302540.6434979/94‑1 (MQ=60)
                                          TTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACA                                                     >  SRR4302540.9390915/1‑94 (MQ=60)
                                          TTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACA                                                     >  SRR4302540.368000/1‑94 (MQ=60)
                                          TTATGAACCCTACAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACA                                                     <  SRR4302540.8905868/94‑1 (MQ=60)
                                           TATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAG                                                    >  SRR4302540.1511451/1‑94 (MQ=60)
                                           TATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAG                                                    <  SRR4302540.9185033/94‑1 (MQ=60)
                                            ATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGA                                                   >  SRR4302540.950453/1‑94 (MQ=60)
                                             TGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG                                                  <  SRR4302540.2247036/94‑1 (MQ=60)
                                             TGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG                                                  <  SRR4302540.9362267/94‑1 (MQ=60)
                                              GAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGA                                                 >  SRR4302540.8706066/1‑94 (MQ=60)
                                              GAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGA                                                 >  SRR4302540.1228918/1‑94 (MQ=60)
                                              GAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGA                                                 >  SRR4302540.5642799/1‑94 (MQ=60)
                                               AACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAA                                                <  SRR4302540.8725502/94‑1 (MQ=60)
                                               AACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAA                                                <  SRR4302540.3965344/94‑1 (MQ=60)
                                               AACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAA                                                <  SRR4302540.7622041/94‑1 (MQ=60)
                                                ACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAG                                               <  SRR4302540.5670257/94‑1 (MQ=60)
                                                ACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAG                                               <  SRR4302540.3029685/94‑1 (MQ=60)
                                                ACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAG                                               >  SRR4302540.7141190/1‑94 (MQ=60)
                                                 CCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGC                                              >  SRR4302540.5321785/1‑94 (MQ=60)
                                                 CCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGC                                              >  SRR4302540.5486204/1‑94 (MQ=60)
                                                  CCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCA                                             >  SRR4302540.1098568/1‑94 (MQ=60)
                                                  CCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCA                                             >  SRR4302540.1098565/1‑94 (MQ=60)
                                                  CCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCA                                             >  SRR4302540.9794739/1‑94 (MQ=60)
                                                   CTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAG                                            <  SRR4302540.8466727/94‑1 (MQ=60)
                                                   CTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAG                                            <  SRR4302540.6708468/94‑1 (MQ=60)
                                                    TCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGC                                           <  SRR4302540.1589546/94‑1 (MQ=60)
                                                    CCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGC                                           <  SRR4302540.3529164/94‑1 (MQ=60)
                                                     CCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCA                                          <  SRR4302540.2442114/94‑1 (MQ=60)
                                                     CCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCA                                          <  SRR4302540.10088073/94‑1 (MQ=60)
                                                      CAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAA                                         >  SRR4302540.3542468/1‑94 (MQ=60)
                                                      CAACGTTCTTATTCTGACCGGTCCTGCCTCCACCATTAACAACCTGATTGAAGGAATAAAGCGGGTTGATGTCATCCGCACAaacaggcggcac                                         >  SRR4302540.3542469/1‑82 (MQ=55)
                                                       AACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAACGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAA                                        >  SRR4302540.2278836/1‑94 (MQ=60)
                                                       AACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAA                                        >  SRR4302540.5837190/1‑94 (MQ=60)
                                                        ACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGCTTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAAT                                       >  SRR4302540.8164927/1‑94 (MQ=60)
                                                        ACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAAT                                       <  SRR4302540.1646540/94‑1 (MQ=60)
                                                        ACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAAT                                       >  SRR4302540.4502998/1‑94 (MQ=60)
                                                         CGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATT                                      >  SRR4302540.7717569/1‑94 (MQ=60)
                                                         CGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATT                                      >  SRR4302540.5755097/1‑94 (MQ=60)
                                                         CGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATT                                      >  SRR4302540.3201742/1‑94 (MQ=60)
                                                          GTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTA                                     <  SRR4302540.1839365/94‑1 (MQ=60)
                                                          GTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTA                                     >  SRR4302540.6834822/1‑94 (MQ=60)
                                                          GTTCTTATTCTGACCGGTCGTGCCCCCACCATTAAAAAACTCTTTGAAGCCATAAAGCGCGTTGATGTCActggcaaagaaaacagcaaaatta                                     >  SRR4302540.7354162/1‑70 (MQ=58)
                                                           TTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTAT                                    <  SRR4302540.1068399/94‑1 (MQ=60)
                                                            TCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATT                                   >  SRR4302540.1806996/1‑94 (MQ=60)
                                                            TCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATT                                   >  SRR4302540.10079483/1‑94 (MQ=60)
                                                            TCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATT                                   >  SRR4302540.2045055/1‑94 (MQ=60)
                                                             CTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTC                                  >  SRR4302540.8841067/1‑94 (MQ=60)
                                                             CTTATTCTGACCGGTCGTGCCTCCAACATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTC                                  >  SRR4302540.4022758/1‑94 (MQ=60)
                                                              TTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCA                                 >  SRR4302540.8267242/1‑94 (MQ=60)
                                                              TTATTATGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCA                                 <  SRR4302540.2683955/94‑1 (MQ=60)
                                                               TATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAT                                >  SRR4302540.177492/1‑94 (MQ=60)
                                                               TATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAT                                <  SRR4302540.10006237/94‑1 (MQ=60)
                                                               TATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAT                                <  SRR4302540.7456594/94‑1 (MQ=60)
                                                                ATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATC                               <  SRR4302540.8701203/94‑1 (MQ=60)
                                                                ATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATC                               >  SRR4302540.8118518/1‑94 (MQ=60)
                                                                ATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATC                               >  SRR4302540.2345489/1‑94 (MQ=60)
                                                                ATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATC                               >  SRR4302540.6039678/1‑94 (MQ=60)
                                                                 TTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCT                              <  SRR4302540.8796932/94‑1 (MQ=60)
                                                                 TTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCT                              >  SRR4302540.9841823/1‑94 (MQ=60)
                                                                 TTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCT                              >  SRR4302540.7446108/1‑94 (MQ=60)
                                                                  TCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTG                             >  SRR4302540.6771892/1‑94 (MQ=60)
                                                                     GACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAA                          >  SRR4302540.2823114/1‑94 (MQ=60)
                                                                      ACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAAT                         <  SRR4302540.7192267/94‑1 (MQ=60)
                                                                        CGGTCGTGCCTCCTCCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATAT                       <  SRR4302540.7196830/94‑1 (MQ=60)
                                                                        CGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATAT                       <  SRR4302540.7262518/94‑1 (MQ=60)
                                                                        CGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATAT                       >  SRR4302540.4624315/1‑94 (MQ=60)
                                                                         GGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATG                      >  SRR4302540.1253962/1‑94 (MQ=60)
                                                                         GGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATG                      >  SRR4302540.7237783/1‑94 (MQ=60)
                                                                         GGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATG                      >  SRR4302540.8971265/1‑94 (MQ=60)
                                                                          GTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGC                     <  SRR4302540.2847550/94‑1 (MQ=60)
                                                                           TCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGAAGCAAATTATTCATCTAGAATATGCG                    >  SRR4302540.645359/1‑94 (MQ=60)
                                                                            CGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGT                   >  SRR4302540.2845057/1‑94 (MQ=60)
                                                                              TGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCA                 >  SRR4302540.5906805/1‑94 (MQ=60)
                                                                              TGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAACTATTCATCTGGAATATGCGTCA                 <  SRR4302540.6093632/94‑1 (MQ=60)
                                                                                CCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGC               >  SRR4302540.2480413/1‑94 (MQ=60)
                                                                                CCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGC               >  SRR4302540.495859/1‑94 (MQ=60)
                                                                                 CTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCG              >  SRR4302540.2866791/1‑94 (MQ=60)
                                                                                 CTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCG              >  SRR4302540.10158756/1‑94 (MQ=60)
                                                                                 CTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCG              >  SRR4302540.4714780/1‑94 (MQ=60)
                                                                                 CTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCG              >  SRR4302540.5432755/1‑94 (MQ=60)
                                                                                 CTCCACCATTAACAAACTGATTGAAGGCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCG              >  SRR4302540.6548308/1‑94 (MQ=60)
                                                                                  TCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGG             >  SRR4302540.8379564/1‑94 (MQ=60)
                                                                                   CCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGA            >  SRR4302540.1521304/1‑94 (MQ=60)
                                                                                   CCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGA            >  SRR4302540.5136788/1‑94 (MQ=60)
                                                                                    CACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAA           >  SRR4302540.3888742/1‑94 (MQ=60)
                                                                                    CACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAA           >  SRR4302540.2671009/1‑94 (MQ=60)
                                                                                     ACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAG          <  SRR4302540.79664/94‑1 (MQ=60)
                                                                                      CCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGA         >  SRR4302540.5751/1‑94 (MQ=60)
                                                                                      CCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGA         <  SRR4302540.61604/94‑1 (MQ=60)
                                                                                       CATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAT        <  SRR4302540.4022399/94‑1 (MQ=60)
                                                                                       CATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAT        >  SRR4302540.3496387/1‑94 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.788861/94‑1 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.1817059/94‑1 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.2621424/94‑1 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.6235933/94‑1 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       >  SRR4302540.4268496/1‑94 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.5127721/94‑1 (MQ=60)
                                                                                        ATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAACAAATTATTCATCTGGAATATGCGTCAGCGGAAGATC       <  SRR4302540.1505039/94‑1 (MQ=60)
                                                                                         TTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT      >  SRR4302540.3315349/1‑94 (MQ=60)
                                                                                         TTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT      <  SRR4302540.8633860/94‑1 (MQ=60)
                                                                                          TAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTC     <  SRR4302540.5803043/94‑1 (MQ=60)
                                                                                          TAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTC     >  SRR4302540.4377124/1‑94 (MQ=60)
                                                                                           AACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCG    >  SRR4302540.9019302/1‑94 (MQ=60)
                                                                                           AACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCG    <  SRR4302540.1750978/94‑1 (MQ=60)
                                                                                             CAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGCAGATCTCGCC  >  SRR4302540.1102899/1‑94 (MQ=60)
                                                                                             CAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCC  <  SRR4302540.5038197/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCC  >  NC_000913/3454445‑3454631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑