Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,496,430 T→C I322I (ATT→ATC)  cysG → uroporphyrinogen III methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,496,4300TC99.4% 1132.8 / ‑3.9 355I322I (ATT→ATC) cysGuroporphyrinogen III methylase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (178/173);  total (180/175)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.25e-01

Only 100 of 371 total aligned reads displayed.

AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG  >  NC_000913/3496340‑3496519
                                                                                          |                                                                                         
aGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATccc                                                                                         >  1:10023554/1‑93 (MQ=255)
aGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATccc                                                                                         >  1:3277629/1‑93 (MQ=255)
aGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATccc                                                                                         <  1:7586766/93‑1 (MQ=255)
 ggCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCg                                                                                        >  1:1107594/1‑93 (MQ=255)
 ggCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCg                                                                                        >  2:6770019/1‑93 (MQ=255)
  gCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGt                                                                                       <  2:662755/93‑1 (MQ=255)
  gCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGt                                                                                       <  1:7412717/93‑1 (MQ=255)
   cAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGtt                                                                                      >  1:3748710/1‑93 (MQ=255)
   cAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGtt                                                                                      <  2:5403947/93‑1 (MQ=255)
     aaCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtct                                                                                    >  1:415096/1‑93 (MQ=255)
      aCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtctc                                                                                   <  1:3684299/93‑1 (MQ=255)
       cgcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCg                                                                                  <  1:4630651/93‑1 (MQ=255)
       cgcgTGGTGCCGCTGAAAGGTGGCGATCCTTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCg                                                                                  <  1:2174172/93‑1 (MQ=255)
        gcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCgg                                                                                 >  1:9582134/1‑93 (MQ=255)
        gcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCgg                                                                                 <  2:2098559/93‑1 (MQ=255)
        gcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCgg                                                                                 >  2:6126260/1‑93 (MQ=255)
           tggtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCggtgg                                                                              >  1:3515409/1‑93 (MQ=255)
             gtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGtt                                                                            >  2:186958/1‑93 (MQ=255)
             gtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGtt                                                                            >  1:6119266/1‑93 (MQ=255)
               gCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTcc                                                                          <  2:6305019/93‑1 (MQ=255)
                cGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCg                                                                         >  1:7810674/1‑93 (MQ=255)
                 gcatGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCgg                                                                        <  2:8072506/90‑1 (MQ=255)
                    tGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTa                                                                     >  2:1135135/1‑93 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAt                                                                    <  1:4283465/93‑1 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAt                                                                    <  1:685261/93‑1 (MQ=255)
                      aaaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAtt                                                                   >  1:5564824/1‑93 (MQ=255)
                      aaaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAtt                                                                   <  1:636059/93‑1 (MQ=255)
                       aaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTa                                                                  >  1:4406784/1‑93 (MQ=255)
                       aaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTa                                                                  <  1:5492093/93‑1 (MQ=255)
                         ggTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTAcc                                                                >  2:4667211/1‑93 (MQ=255)
                           tGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGc                                                              >  1:3062908/1‑93 (MQ=255)
                           tGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGc                                                              >  2:4254304/1‑93 (MQ=255)
                           tGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGGGGTTCCGGGGATTACCGc                                                              >  2:3453938/1‑93 (MQ=255)
                            ggCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCa                                                             <  1:7768091/93‑1 (MQ=255)
                             gCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAg                                                            <  2:4394611/93‑1 (MQ=255)
                              cGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACTCTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGc                                                           <  1:2350657/93‑1 (MQ=255)
                              cGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGc                                                           >  1:8860813/1‑93 (MQ=255)
                              cGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGc                                                           >  2:3160326/1‑93 (MQ=255)
                               gATCCGTTTATTTTTGGCCGTGGTAGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCt                                                          >  2:2245867/1‑93 (MQ=255)
                                aTCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCtt                                                         >  1:698954/1‑93 (MQ=255)
                                aTCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCtt                                                         >  1:1500009/1‑93 (MQ=255)
                                  ccGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCt                                                       <  1:1936092/93‑1 (MQ=255)
                                  ccGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCt                                                       >  2:9517381/1‑93 (MQ=255)
                                   cGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTg                                                      <  2:7415389/93‑1 (MQ=255)
                                   cGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTg                                                      <  2:6405445/93‑1 (MQ=255)
                                    gTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTgg                                                     >  1:577267/1‑93 (MQ=255)
                                    gTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTgg                                                     >  1:7118012/1‑93 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGt                                                    >  1:813813/1‑93 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGt                                                    >  1:2634526/1‑93 (MQ=255)
                                      ttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGtt                                                   <  2:7665097/93‑1 (MQ=255)
                                      ttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGtt                                                   <  2:1308449/93‑1 (MQ=255)
                                       tATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTg                                                  >  2:5575615/1‑93 (MQ=255)
                                         tttttGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGct                                                >  1:5051685/1‑93 (MQ=255)
                                          ttttGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGctc                                               >  1:6742467/1‑93 (MQ=255)
                                          ttttGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGctc                                               <  2:7487378/93‑1 (MQ=255)
                                             tGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATACCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGc                                            <  2:3720105/93‑1 (MQ=255)
                                               gCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCt                                          <  2:4592149/93‑1 (MQ=255)
                                                 cGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTAt                                        <  1:8506953/93‑1 (MQ=255)
                                                 cGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTAt                                        <  1:5387640/93‑1 (MQ=255)
                                                  gtggtgGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTAtt                                       >  1:3226629/1‑93 (MQ=255)
                                                      tggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCggg                                   >  1:82195/1‑93 (MQ=255)
                                                        gCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTa                                 <  2:2448471/93‑1 (MQ=255)
                                                        gCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTa                                 <  2:8722685/93‑1 (MQ=255)
                                                          gAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAtt                               >  1:4459196/1‑93 (MQ=255)
                                                          gAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAtt                               >  1:8676557/1‑93 (MQ=255)
                                                           aaGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTc                              <  2:8150358/93‑1 (MQ=255)
                                                           aaGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTc                              >  1:6622473/1‑93 (MQ=255)
                                                           aaGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTc                              >  2:5040698/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTTGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             >  2:8426210/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             >  2:6823165/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             >  2:5979298/1‑93 (MQ=255)
                                                             gagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCa                            >  2:9311425/1‑93 (MQ=255)
                                                              agCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAc                           >  1:1685047/1‑93 (MQ=255)
                                                              agCTGGAAACACTGTGCAACGCGCGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAc                           <  2:5502572/93‑1 (MQ=255)
                                                                 tGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCa                        >  1:5266619/1‑93 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAc                       >  1:9514036/1‑93 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAc                       >  1:6224368/1‑93 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAc                       <  1:1738528/93‑1 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGAATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAATCag                       >  1:9038593/1‑92 (MQ=255)
                                                                   gAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACg                      >  2:7071754/1‑93 (MQ=255)
                                                                    aaaCACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGc                     >  1:1124313/1‑93 (MQ=255)
                                                                     aaCACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCa                    <  2:7977470/93‑1 (MQ=255)
                                                                      acacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAt                   <  1:342244/93‑1 (MQ=255)
                                                                      acacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAt                   >  2:864578/1‑93 (MQ=255)
                                                                       cacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATc                  <  2:9751988/93‑1 (MQ=255)
                                                                        acTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcg                 >  2:8283316/1‑93 (MQ=255)
                                                                         cTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcgc                >  2:3014538/1‑93 (MQ=255)
                                                                          tgtgCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcgcg               >  1:7932835/1‑93 (MQ=255)
                                                                          tgtgCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcgcg               >  1:1823059/1‑93 (MQ=255)
                                                                             gcgaCGCGGGTCACCCGATATCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGAtt            <  2:8971237/90‑1 (MQ=255)
                                                                              cAACGCGGGTATCCCGTTCTGTGTGGTTCCGGGTATTACCGCGGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:272793/93‑1 (MQ=255)
                                                                              cAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:5328195/93‑1 (MQ=255)
                                                                              cAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:4746529/93‑1 (MQ=255)
                                                                               aaCGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAt          <  2:1686279/93‑1 (MQ=255)
                                                                                aCGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATg         >  2:5198166/1‑93 (MQ=255)
                                                                                 cgcgGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGc        <  1:2832663/93‑1 (MQ=255)
                                                                                     ggTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCag    >  2:1471922/1‑93 (MQ=255)
                                                                                     ggTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCag    >  1:7230860/1‑93 (MQ=255)
                                                                                      gTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCaga   <  2:8886950/93‑1 (MQ=255)
                                                                                       tATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCagag  >  1:9248598/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG  >  NC_000913/3496340‑3496519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  NC_000913/3496337‑3496523
                                                                                             |                                                                                             
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATC                                                                                               >  SRR4302540.7350253/1‑94 (MQ=60)
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATC                                                                                               >  SRR4302540.3259840/1‑94 (MQ=60)
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATC                                                                                               <  SRR4302540.3109037/94‑1 (MQ=60)
 AAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCC                                                                                              >  SRR4302540.60438/1‑94 (MQ=60)
 AAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCC                                                                                              <  SRR4302540.3706241/94‑1 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             >  SRR4302540.10140189/1‑94 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             >  SRR4302540.3313426/1‑94 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             <  SRR4302540.1793615/94‑1 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             <  SRR4302540.8923650/94‑1 (MQ=60)
   AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCG                                                                                            >  SRR4302540.4674368/1‑94 (MQ=60)
   AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCG                                                                                            >  SRR4302540.1119035/1‑94 (MQ=60)
   AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCG                                                                                            <  SRR4302540.7671960/94‑1 (MQ=60)
   AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCG                                                                                            <  SRR4302540.872324/94‑1 (MQ=60)
     GCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTT                                                                                          >  SRR4302540.3789614/1‑94 (MQ=60)
     GCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTT                                                                                          <  SRR4302540.7495281/94‑1 (MQ=60)
     GCAAACGCGTGGCGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTT                                                                                          >  SRR4302540.1912983/1‑94 (MQ=60)
      CAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTC                                                                                         <  SRR4302540.3521352/94‑1 (MQ=60)
      CAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTC                                                                                         >  SRR4302540.9092756/1‑94 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        <  SRR4302540.5793796/94‑1 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        <  SRR4302540.6176356/94‑1 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        >  SRR4302540.419444/1‑94 (MQ=60)
        AACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTC                                                                                       <  SRR4302540.3093634/94‑1 (MQ=60)
        AACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTC                                                                                       <  SRR4302540.5551642/94‑1 (MQ=60)
         ACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCG                                                                                      <  SRR4302540.3724561/94‑1 (MQ=60)
         ACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCG                                                                                      <  SRR4302540.3743932/94‑1 (MQ=60)
         ACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCG                                                                                      <  SRR4302540.172587/94‑1 (MQ=60)
          CGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGG                                                                                     >  SRR4302540.421735/1‑94 (MQ=60)
          CGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGG                                                                                     >  SRR4302540.9695281/1‑94 (MQ=60)
          CGCGTGGTGCCGCTGAAAGGTGGCGATCCTTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGG                                                                                     <  SRR4302540.2197620/94‑1 (MQ=60)
           GCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGT                                                                                    <  SRR4302540.1829566/94‑1 (MQ=60)
           GCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGT                                                                                    <  SRR4302540.3472108/94‑1 (MQ=60)
            CGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTG                                                                                   >  SRR4302540.8694736/1‑94 (MQ=60)
             GTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGG                                                                                  >  SRR4302540.3553707/1‑94 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                <  SRR4302540.6826179/94‑1 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                >  SRR4302540.6187415/1‑94 (MQ=60)
                 TGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCC                                                                              <  SRR4302540.3217534/94‑1 (MQ=60)
                 TGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCC                                                                              <  SRR4302540.1773943/94‑1 (MQ=60)
                  GCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCG                                                                             >  SRR4302540.7901946/1‑94 (MQ=60)
                   CGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGG                                                                            <  SRR4302540.4301328/94‑1 (MQ=60)
                   CGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGG                                                                            <  SRR4302540.5531216/94‑1 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           <  SRR4302540.3303657/94‑1 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           >  SRR4302540.2212257/1‑94 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           >  SRR4302540.2003642/1‑94 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           >  SRR4302540.5791978/1‑94 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           >  SRR4302540.4347224/1‑94 (MQ=60)
                     GCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGT                                                                          <  SRR4302540.6859450/94‑1 (MQ=60)
                      CTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTA                                                                         <  SRR4302540.7419378/94‑1 (MQ=60)
                      CTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTA                                                                         >  SRR4302540.10159323/1‑94 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAT                                                                        <  SRR4302540.2691411/94‑1 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAT                                                                        <  SRR4302540.2552357/94‑1 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTGTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTAT                                                                        <  SRR4302540.79505/94‑1 (MQ=60)
                        GAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATT                                                                       <  SRR4302540.692616/94‑1 (MQ=60)
                        GAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATT                                                                       >  SRR4302540.5626099/1‑94 (MQ=60)
                        GAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATT                                                                       <  SRR4302540.4330060/94‑1 (MQ=60)
                        GAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATT                                                                       >  SRR4302540.5626100/1‑94 (MQ=60)
                         AAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTA                                                                      >  SRR4302540.7648443/1‑94 (MQ=60)
                         AAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTA                                                                      <  SRR4302540.642820/94‑1 (MQ=60)
                         AAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTA                                                                      >  SRR4302540.4454556/1‑94 (MQ=60)
                          AAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTAC                                                                     >  SRR4302540.6503578/1‑94 (MQ=60)
                          AAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTAC                                                                     <  SRR4302540.5552564/94‑1 (MQ=60)
                          AAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTAC                                                                     >  SRR4302540.1348317/1‑94 (MQ=60)
                          AAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTAC                                                                     <  SRR4302540.1756253/94‑1 (MQ=60)
                           AGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACC                                                                    <  SRR4302540.1621682/94‑1 (MQ=60)
                           AGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACC                                                                    <  SRR4302540.5275489/94‑1 (MQ=60)
                           AGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACC                                                                    <  SRR4302540.8311604/94‑1 (MQ=60)
                             GTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGC                                                                  >  SRR4302540.3096509/1‑94 (MQ=60)
                              TGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCA                                                                 >  SRR4302540.7600719/1‑94 (MQ=60)
                               GGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAG                                                                <  SRR4302540.7858905/94‑1 (MQ=60)
                               GGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAG                                                                <  SRR4302540.6786511/94‑1 (MQ=60)
                                GCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGC                                                               >  SRR4302540.8963344/1‑94 (MQ=60)
                                GCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGC                                                               >  SRR4302540.8963345/1‑94 (MQ=60)
                                 CGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACTCTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCT                                                              <  SRR4302540.2375967/94‑1 (MQ=60)
                                 CGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCT                                                              <  SRR4302540.5072237/94‑1 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTT                                                             >  SRR4302540.9566711/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTT                                                             >  SRR4302540.1516534/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTT                                                             >  SRR4302540.706453/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTT                                                             >  SRR4302540.7647652/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTT                                                             >  SRR4302540.706451/1‑94 (MQ=60)
                                   ATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTC                                                            >  SRR4302540.1171015/1‑94 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCT                                                           >  SRR4302540.1365412/1‑94 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCT                                                           <  SRR4302540.3297646/94‑1 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCG                                                           >  SRR4302540.6764980/1‑94 (MQ=60)
                                    TCCGTTTATGTGTGGCCGTGGTGGCGGAGAGCTGGAAACACTGTGCAACGCGGGTAGCCCGTTCTCGGTGCTTCCGGGTATTACCGCAGCTTCT                                                           <  SRR4302540.201638/94‑1 (MQ=60)
                                     CCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTG                                                          >  SRR4302540.7291159/1‑94 (MQ=60)
                                     CCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTG                                                          <  SRR4302540.1957184/94‑1 (MQ=60)
                                      CGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGG                                                         >  SRR4302540.583519/1‑94 (MQ=60)
                                      CGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGG                                                         >  SRR4302540.7197253/1‑94 (MQ=60)
                                      CGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGG                                                         >  SRR4302540.7132610/1‑94 (MQ=60)
                                      CGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGGATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGG                                                         >  SRR4302540.2557877/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.5372045/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        <  SRR4302540.5359532/94‑1 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.9747026/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        <  SRR4302540.4671544/94‑1 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.9224402/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.822455/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.2663053/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.7945521/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.3447971/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGT                                                        >  SRR4302540.7387026/1‑94 (MQ=60)
                                         TTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTG                                                      >  SRR4302540.3440133/1‑94 (MQ=60)
                                         TTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTG                                                      <  SRR4302540.782130/94‑1 (MQ=60)
                                         TTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTG                                                      <  SRR4302540.5737702/94‑1 (MQ=60)
                                          TATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGC                                                     >  SRR4302540.5601340/1‑94 (MQ=60)
                                           ATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCT                                                    <  SRR4302540.8626762/94‑1 (MQ=60)
                                           ATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCT                                                    >  SRR4302540.5106302/1‑94 (MQ=60)
                                            TTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTC                                                   <  SRR4302540.4070674/94‑1 (MQ=60)
                                            TTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTC                                                   >  SRR4302540.6816960/1‑94 (MQ=60)
                                             TTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCT                                                  <  SRR4302540.1749478/94‑1 (MQ=60)
                                             TTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCT                                                  <  SRR4302540.8605325/94‑1 (MQ=60)
                                             TTTTGGCCGTGGTGGAGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTTGGTGTTTCCGTGTATTACCGCAGCTTCTGGTTGCTCT                                                  <  SRR4302540.5498264/94‑1 (MQ=60)
                                               TTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGC                                                <  SRR4302540.6713400/94‑1 (MQ=60)
                                                 GGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCT                                              <  SRR4302540.7308383/94‑1 (MQ=60)
                                                   CCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTAT                                            <  SRR4302540.117270/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           >  SRR4302540.3261706/1‑94 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302540.5446894/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302540.5446893/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           >  SRR4302540.9231885/1‑94 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302540.8605815/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302540.6618474/94‑1 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          >  SRR4302540.7730051/1‑94 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          <  SRR4302540.8035525/94‑1 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          <  SRR4302540.4327644/94‑1 (MQ=60)
                                                      TGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCG                                         <  SRR4302540.9651111/94‑1 (MQ=60)
                                                      TGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCG                                         <  SRR4302540.8441552/94‑1 (MQ=60)
                                                      TGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCACCTTCTGGTTGCTCTGCCTATTCG                                         <  SRR4302540.1843114/94‑1 (MQ=60)
                                                        GTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGG                                       >  SRR4302540.83059/1‑94 (MQ=60)
                                                         TGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGT                                      <  SRR4302540.5864799/94‑1 (MQ=60)
                                                         TGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGT                                      <  SRR4302540.7874816/94‑1 (MQ=60)
                                                         TGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGT                                      <  SRR4302540.1121130/94‑1 (MQ=60)
                                                          GGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGGA                                     >  SRR4302540.7919628/1‑94 (MQ=60)
                                                           GCGAAGAGCTGGAAGCACTGTGCAACGCGTGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAT                                    <  SRR4302540.1636437/94‑1 (MQ=60)
                                                           GCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAT                                    <  SRR4302540.5258103/94‑1 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   >  SRR4302540.8777330/1‑94 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   >  SRR4302540.4507538/1‑94 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTTTGCCTATTCGGGTATTC                                  >  SRR4302540.4333809/1‑94 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  >  SRR4302540.6695798/1‑94 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  >  SRR4302540.3568301/1‑94 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  >  SRR4302540.497151/1‑94 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  >  SRR4302540.6319211/1‑94 (MQ=60)
                                                                GAGCTGTAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAC                               <  SRR4302540.2750164/94‑1 (MQ=60)
                                                                GAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAC                               >  SRR4302540.1703330/1‑94 (MQ=60)
                                                                GAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAC                               >  SRR4302540.8347718/1‑94 (MQ=60)
                                                                GAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAC                               <  SRR4302540.352928/94‑1 (MQ=60)
                                                                 AGCTCGCAACACTGTGTAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACT                              <  SRR4302540.3386248/94‑1 (MQ=60)
                                                                  GCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTC                             <  SRR4302540.3181625/94‑1 (MQ=60)
                                                                   CTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCA                            >  SRR4302540.5324095/1‑94 (MQ=60)
                                                                   CTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCA                            >  SRR4302540.4480899/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302540.9626729/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302540.304530/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302540.304529/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302540.450420/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           <  SRR4302540.7027964/94‑1 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302540.6293596/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGAATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAatcag                           >  SRR4302540.9147085/1‑89 (MQ=60)
                                                                     GGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACG                          <  SRR4302540.1757301/94‑1 (MQ=60)
                                                                     GGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACC                          >  SRR4302540.7346517/1‑94 (MQ=60)
                                                                      GAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGC                         >  SRR4302540.1136004/1‑94 (MQ=60)
                                                                      GAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGC                         >  SRR4302540.1136003/1‑94 (MQ=60)
                                                                      GAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGC                         >  SRR4302540.9225940/1‑94 (MQ=60)
                                                                      GAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGC                         >  SRR4302540.3772629/1‑94 (MQ=60)
                                                                       AAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCA                        <  SRR4302540.4622696/94‑1 (MQ=60)
                                                                        AACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAT                       >  SRR4302540.8191692/1‑94 (MQ=60)
                                                                         ACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATC                      <  SRR4302540.345708/94‑1 (MQ=60)
                                                                          CACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCG                     <  SRR4302540.10048308/94‑1 (MQ=60)
                                                                          CACTGTGCAACGCGGGTATCCCGTTCTCGGTGGCTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCG                     >  SRR4302540.4902225/1‑94 (MQ=60)
                                                                           ACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGC                    >  SRR4302540.9405244/1‑94 (MQ=60)
                                                                           ACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGC                    <  SRR4302540.2605409/94‑1 (MQ=60)
                                                                            CTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCG                   >  SRR4302540.8025295/1‑94 (MQ=60)
                                                                            CTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCG                   >  SRR4302540.1842981/1‑94 (MQ=60)
                                                                               TGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATT                >  SRR4302540.8179375/1‑94 (MQ=60)
                                                                                 GAACGCGAGTATACCGTTCTCGGTGGTTCCTGGCAGTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302540.7174367/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302540.7100791/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              >  SRR4302540.8942571/1‑94 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302540.770585/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302540.6212468/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              >  SRR4302540.8302627/1‑94 (MQ=60)
                                                                                  AACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATG             >  SRR4302540.2031920/1‑94 (MQ=60)
                                                                                  AACGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATG             <  SRR4302540.9471492/94‑1 (MQ=60)
                                                                                    CGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCC           >  SRR4302540.4459731/1‑94 (MQ=60)
                                                                                    CGCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCC           <  SRR4302540.2863298/94‑1 (MQ=60)
                                                                                     GCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCC          <  SRR4302540.1121335/94‑1 (MQ=60)
                                                                                     GCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCC          <  SRR4302540.3154163/94‑1 (MQ=60)
                                                                                     GCGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCC          <  SRR4302540.2327208/94‑1 (MQ=60)
                                                                                     GCGGGTATCCCGTTCTCGGTGGTTACGGGTATTACCGCCGATTCTGGATGCTCTGCCTATTCGGGTAATCCTCTCATGCATCGTGACTATGCCC          >  SRR4302540.9869725/1‑94 (MQ=42)
                                                                                      CGGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCA         >  SRR4302540.4074571/1‑94 (MQ=60)
                                                                                       GGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAG        <  SRR4302540.6293125/94‑1 (MQ=60)
                                                                                       GGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAG        >  SRR4302540.962450/1‑94 (MQ=60)
                                                                                       GGGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAG        >  SRR4302540.7311496/1‑94 (MQ=60)
                                                                                        GGTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGA       <  SRR4302540.6378828/94‑1 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302540.6586043/94‑1 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302540.1025738/94‑1 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      >  SRR4302540.3703454/1‑94 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302540.2340978/94‑1 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      >  SRR4302540.9359326/1‑94 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      >  SRR4302540.8940190/1‑94 (MQ=60)
                                                                                         GTATCCCGTGCTCGGGGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302540.3814709/94‑1 (MQ=60)
                                                                                          TATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGC     <  SRR4302540.6200477/94‑1 (MQ=60)
                                                                                          TATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGC     >  SRR4302540.828964/1‑94 (MQ=60)
                                                                                          TATCCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGC     >  SRR4302540.9758821/1‑94 (MQ=60)
                                                                                             CCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  <  SRR4302540.9481279/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  NC_000913/3496337‑3496523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑