Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,507,831 T→C T262A (ACT→GCT)  yhfX ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,507,8310TC99.2% 1224.8 / ‑3.3 381T262A (ACT→GCT) yhfXhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (186/192);  total (187/194)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 391 total aligned reads displayed.

AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGT  >  NC_000913/3507741‑3507920
                                                                                          |                                                                                         
aGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGAGc                                                                                         >  1:5255045/1‑93 (MQ=255)
  cTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGccc                                                                                       <  2:8151877/93‑1 (MQ=255)
    gTCGCGACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGt                                                                                     <  2:5491816/93‑1 (MQ=255)
     tCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTc                                                                                    <  1:3632543/93‑1 (MQ=255)
     tCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTc                                                                                    >  2:3353042/1‑93 (MQ=255)
      cGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCa                                                                                   <  2:9189123/93‑1 (MQ=255)
          aCGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgcgc                                                                               >  2:4254862/1‑93 (MQ=255)
          aCGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgcgc                                                                               <  1:682078/93‑1 (MQ=255)
           cGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCa                                                                              <  1:8599435/93‑1 (MQ=255)
           cGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCa                                                                              >  1:7391413/1‑93 (MQ=255)
            ggAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAt                                                                             <  2:612225/93‑1 (MQ=255)
              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  1:5918841/93‑1 (MQ=255)
              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  2:2029909/93‑1 (MQ=255)
              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  1:1072711/93‑1 (MQ=255)
               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                          >  1:279028/1‑93 (MQ=255)
               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                          <  2:5602161/93‑1 (MQ=255)
                atgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGTGCATGAcc                                                                         <  2:2826531/93‑1 (MQ=255)
                atgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAcc                                                                         <  2:8030751/93‑1 (MQ=255)
                 tgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCg                                                                        >  1:4600757/1‑93 (MQ=255)
                  gatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCgg                                                                       >  1:5490881/1‑93 (MQ=255)
                  gatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGAATGACCgg                                                                       >  1:556171/1‑93 (MQ=255)
                   atgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCggg                                                                      <  1:9554114/93‑1 (MQ=255)
                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGt                                                                     <  2:5293452/93‑1 (MQ=255)
                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGt                                                                     <  2:488944/93‑1 (MQ=255)
                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  1:6063992/93‑1 (MQ=255)
                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  2:4220427/93‑1 (MQ=255)
                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    >  1:672928/1‑93 (MQ=255)
                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  2:9581923/93‑1 (MQ=255)
                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                                   >  1:2275553/1‑93 (MQ=255)
                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                                   <  1:7143323/93‑1 (MQ=255)
                       gagaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCg                                                                  <  2:5754659/93‑1 (MQ=255)
                       gagaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCg                                                                  <  1:6004341/93‑1 (MQ=255)
                        agaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCgg                                                                 <  1:8046077/93‑1 (MQ=255)
                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               <  2:3284256/93‑1 (MQ=255)
                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               >  2:7535517/1‑93 (MQ=255)
                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAAGCTGAACGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               <  2:8949484/93‑1 (MQ=255)
                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                              >  2:2449366/1‑93 (MQ=255)
                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                              >  2:9759856/1‑93 (MQ=255)
                            ttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATg                                                             >  1:9048288/1‑93 (MQ=255)
                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            <  2:7653092/93‑1 (MQ=255)
                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            >  1:8830660/1‑93 (MQ=255)
                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            >  2:1414226/1‑93 (MQ=255)
                               aCTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGt                                                          >  1:1716208/1‑93 (MQ=255)
                               aCTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGt                                                          >  1:6200529/1‑93 (MQ=255)
                                cTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTc                                                         <  1:5314822/93‑1 (MQ=255)
                                     ccAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACAcc                                                    <  2:4620730/93‑1 (MQ=255)
                                     ccAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACAcc                                                    <  2:1033823/93‑1 (MQ=255)
                                       agagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGt                                                  >  2:1772287/1‑93 (MQ=255)
                                       agagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGt                                                  >  2:5893552/1‑93 (MQ=255)
                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                                 <  1:2612479/93‑1 (MQ=255)
                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                                 <  2:125985/93‑1 (MQ=255)
                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                               <  2:3876831/93‑1 (MQ=255)
                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                               >  1:8472941/1‑93 (MQ=255)
                                            aTCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgc                                             >  2:9329715/1‑93 (MQ=255)
                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgcg                                            >  1:9134966/1‑93 (MQ=255)
                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAGTgcg                                            >  2:7128136/1‑93 (MQ=255)
                                               gcgATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGAATGAGTCACACCGTATTGCGcc                                          <  1:1838062/93‑1 (MQ=255)
                                                 gATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAg                                        <  1:9722909/93‑1 (MQ=255)
                                                 gATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAg                                        >  2:6355439/1‑93 (MQ=255)
                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:3389383/1‑93 (MQ=255)
                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:2712639/1‑93 (MQ=255)
                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:2061728/1‑93 (MQ=255)
                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGACACACCGAATTGCGCCAGt                                       >  1:8641819/1‑93 (MQ=255)
                                                   tACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTa                                      <  2:8809310/93‑1 (MQ=255)
                                                   tACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTa                                      <  2:159500/93‑1 (MQ=255)
                                                    aCGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTaa                                     <  2:7936282/93‑1 (MQ=255)
                                                    aCGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTaa                                     <  2:5581757/93‑1 (MQ=255)
                                                      gTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACTCCGTATTGCGCCAGTAATg                                   >  2:8684136/1‑93 (MQ=255)
                                                      gTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTACTg                                   >  2:8400646/1‑93 (MQ=255)
                                                       ttCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATgg                                  <  1:9857808/93‑1 (MQ=255)
                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 >  2:1096355/1‑93 (MQ=255)
                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:6978470/93‑1 (MQ=255)
                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  2:2273955/93‑1 (MQ=255)
                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:7691913/93‑1 (MQ=255)
                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:727667/93‑1 (MQ=255)
                                                          aGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAg                               >  2:5986003/1‑93 (MQ=255)
                                                           ggCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGc                              <  1:1331014/93‑1 (MQ=255)
                                                               gATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAg                          <  1:4374786/93‑1 (MQ=255)
                                                               gATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAg                          <  2:1680413/93‑1 (MQ=255)
                                                                aTCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGt                         <  1:4097963/93‑1 (MQ=255)
                                                                 tCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTg                        <  2:7329277/93‑1 (MQ=255)
                                                                 tCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTg                        >  2:661289/1‑93 (MQ=255)
                                                                  cGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGc                       <  1:4426345/93‑1 (MQ=255)
                                                                   gCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCa                      <  2:3558885/93‑1 (MQ=255)
                                                                   gCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCa                      <  1:4946980/93‑1 (MQ=255)
                                                                    cccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAg                     <  2:6976776/93‑1 (MQ=255)
                                                                    cccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAg                     >  2:271532/1‑93 (MQ=255)
                                                                     ccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGc                    <  2:8600208/93‑1 (MQ=255)
                                                                      ctgctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCt                   >  1:4734688/1‑93 (MQ=255)
                                                                      ctgctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGGTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGcg                   >  2:5185667/1‑92 (MQ=255)
                                                                        gctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTgg                 <  2:645119/93‑1 (MQ=255)
                                                                         ctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGt                <  1:1162940/93‑1 (MQ=255)
                                                                         ctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGt                <  1:5037913/93‑1 (MQ=255)
                                                                          tgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTc               >  2:5326925/1‑93 (MQ=255)
                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            <  1:6306693/93‑1 (MQ=255)
                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            >  1:3127792/1‑93 (MQ=255)
                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            <  2:1971604/93‑1 (MQ=255)
                                                                                gCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGaa         <  1:3249183/93‑1 (MQ=255)
                                                                                 ccGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAg        <  1:3820128/93‑1 (MQ=255)
                                                                                  cGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAgg       >  1:8401348/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGT  >  NC_000913/3507741‑3507920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 205 total aligned reads displayed.
AGTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAG  >  NC_000913/3507738‑3507924
                                                                                             |                                                                                             
AGTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGC                                                                                               >  SRR4302540.3544853/1‑94 (MQ=60)
 GTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCG                                                                                              <  SRR4302540.1468049/94‑1 (MQ=60)
 GTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCG                                                                                              >  SRR4302540.8237660/1‑94 (MQ=60)
 GTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCG                                                                                              >  SRR4302540.1232157/1‑94 (MQ=60)
 GTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAACATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCTGAATAGCG                                                                                              >  SRR4302540.8077901/1‑94 (MQ=60)
  TAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGAGC                                                                                             >  SRR4302540.5312359/1‑94 (MQ=60)
   AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCC                                                                                            <  SRR4302540.8926857/94‑1 (MQ=60)
    GCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCC                                                                                           <  SRR4302540.9835341/94‑1 (MQ=60)
    GCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCC                                                                                           <  SRR4302540.3219470/94‑1 (MQ=60)
    GCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCC                                                                                           >  SRR4302540.5635799/1‑94 (MQ=60)
    GCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCC                                                                                           <  SRR4302540.6770437/94‑1 (MQ=60)
      TGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGT                                                                                         >  SRR4302540.1751905/1‑94 (MQ=60)
      TGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGT                                                                                         >  SRR4302540.6106468/1‑94 (MQ=60)
       GTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTC                                                                                        >  SRR4302540.2776/1‑94 (MQ=60)
        TCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCA                                                                                       <  SRR4302540.8710959/94‑1 (MQ=60)
        TCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCA                                                                                       <  SRR4302540.3672305/94‑1 (MQ=60)
           CCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCG                                                                                    >  SRR4302540.8119368/1‑94 (MQ=60)
             ACGGAAATGATGGGAGATTTCACTTAACCTGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCA                                                                                  <  SRR4302540.5594977/94‑1 (MQ=60)
             ACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCA                                                                                  <  SRR4302540.689684/94‑1 (MQ=60)
             ACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCA                                                                                  <  SRR4302540.689387/94‑1 (MQ=60)
             ACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCA                                                                                  >  SRR4302540.7093050/1‑94 (MQ=60)
             ACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCA                                                                                  >  SRR4302540.7473774/1‑94 (MQ=60)
              CGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAT                                                                                 >  SRR4302540.2494488/1‑94 (MQ=60)
              CGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAT                                                                                 <  SRR4302540.8699388/94‑1 (MQ=60)
              CGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAT                                                                                 <  SRR4302540.9933167/94‑1 (MQ=60)
               GGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATG                                                                                >  SRR4302540.4969392/1‑94 (MQ=60)
               GGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATG                                                                                <  SRR4302540.10104060/94‑1 (MQ=60)
                 AAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAC                                                                              >  SRR4302540.4895518/1‑94 (MQ=60)
                 AAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAC                                                                              <  SRR4302540.5984224/94‑1 (MQ=60)
                 AAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAC                                                                              <  SRR4302540.1083657/94‑1 (MQ=60)
                 AAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAC                                                                              <  SRR4302540.1083658/94‑1 (MQ=60)
                  AATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACC                                                                             <  SRR4302540.3976922/94‑1 (MQ=60)
                  AATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACC                                                                             <  SRR4302540.4034837/94‑1 (MQ=60)
                  AATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACC                                                                             <  SRR4302540.5176461/94‑1 (MQ=60)
                   ATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCG                                                                            <  SRR4302540.1374814/94‑1 (MQ=60)
                   ATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCG                                                                            >  SRR4302540.4650202/1‑94 (MQ=60)
                    TGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATGGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGG                                                                           <  SRR4302540.8725615/94‑1 (MQ=60)
                    TGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGG                                                                           <  SRR4302540.8725616/94‑1 (MQ=60)
                    TGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGG                                                                           <  SRR4302540.552464/94‑1 (MQ=60)
                    TGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGG                                                                           >  SRR4302540.5551332/1‑94 (MQ=60)
                    TGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGAATGACCGG                                                                           >  SRR4302540.562235/1‑94 (MQ=60)
                     GATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGG                                                                          >  SRR4302540.3851247/1‑94 (MQ=60)
                     GATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGG                                                                          <  SRR4302540.6057075/94‑1 (MQ=60)
                      ATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGT                                                                         >  SRR4302540.6189809/1‑94 (MQ=60)
                      ATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGT                                                                         >  SRR4302540.6189808/1‑94 (MQ=60)
                      ATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGT                                                                         <  SRR4302540.9667112/94‑1 (MQ=60)
                      ATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGT                                                                         <  SRR4302540.9748858/94‑1 (MQ=60)
                       TGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTT                                                                        >  SRR4302540.680135/1‑94 (MQ=60)
                       TGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTT                                                                        >  SRR4302540.2450361/1‑94 (MQ=60)
                       TGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTT                                                                        <  SRR4302540.9414454/94‑1 (MQ=60)
                       TGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTT                                                                        <  SRR4302540.5051717/94‑1 (MQ=60)
                       TGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTT                                                                        <  SRR4302540.9910049/94‑1 (MQ=60)
                        GGGAGATTTCACTTCACCAGAGCATCGCGATACGTTCAGGCTGATCGCGCTGCTGGTTTGGCGGAATAGCGCCCCTCAGCCAATGACCGGGTTC                                                                       >  SRR4302540.2299982/1‑94 (MQ=60)
                        GGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTC                                                                       >  SRR4302540.8781390/1‑94 (MQ=60)
                        GGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTC                                                                       >  SRR4302540.2299987/1‑94 (MQ=60)
                        GGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTC                                                                       >  SRR4302540.7379047/1‑94 (MQ=60)
                         GGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCG                                                                      >  SRR4302540.3833256/1‑94 (MQ=60)
                         GGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCG                                                                      <  SRR4302540.7222911/94‑1 (MQ=60)
                         GGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCG                                                                      >  SRR4302540.2722435/1‑94 (MQ=60)
                         GGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCG                                                                      <  SRR4302540.1213553/94‑1 (MQ=60)
                          GAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGG                                                                     <  SRR4302540.6070904/94‑1 (MQ=60)
                           AGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGC                                                                    <  SRR4302540.9853796/94‑1 (MQ=60)
                           AGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGC                                                                    <  SRR4302540.8139552/94‑1 (MQ=60)
                            GATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCA                                                                   >  SRR4302540.6815546/1‑94 (MQ=60)
                            GATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGGTCGGCA                                                                   >  SRR4302540.3241636/1‑94 (MQ=60)
                             ATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAT                                                                  <  SRR4302540.7427085/94‑1 (MQ=60)
                              TTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATG                                                                 >  SRR4302540.5020291/1‑94 (MQ=60)
                              TTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATG                                                                 >  SRR4302540.9156910/1‑94 (MQ=60)
                              TTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATG                                                                 >  SRR4302540.4258768/1‑94 (MQ=60)
                               TTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGA                                                                >  SRR4302540.8932790/1‑94 (MQ=60)
                                TCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAG                                                               >  SRR4302540.8982451/1‑94 (MQ=60)
                                TCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAG                                                               >  SRR4302540.6104450/1‑94 (MQ=60)
                                 CACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGT                                                              >  SRR4302540.6269556/1‑94 (MQ=60)
                                 CACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGT                                                              <  SRR4302540.1999940/94‑1 (MQ=60)
                                 CACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGT                                                              >  SRR4302540.1734738/1‑94 (MQ=60)
                                 CACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTACTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGT                                                              >  SRR4302540.9201347/1‑94 (MQ=60)
                                   CTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCA                                                            >  SRR4302540.2863060/1‑94 (MQ=60)
                                   CTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCA                                                            <  SRR4302540.5373019/94‑1 (MQ=60)
                                   CTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCA                                                            <  SRR4302540.6969052/94‑1 (MQ=60)
                                   CTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCACCGCATGACCGGGTTCGGCCTGAGTCC                                                            >  SRR4302540.8675190/1‑94 (MQ=60)
                                    TTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCAC                                                           <  SRR4302540.3771449/94‑1 (MQ=60)
                                     TAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCGCA                                                          >  SRR4302540.6369133/1‑94 (MQ=60)
                                     TAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACA                                                          >  SRR4302540.6369132/1‑94 (MQ=60)
                                     TAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACA                                                          <  SRR4302540.607615/94‑1 (MQ=60)
                                     TAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACA                                                          >  SRR4302540.313691/1‑94 (MQ=60)
                                       ACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACC                                                        <  SRR4302540.8643974/94‑1 (MQ=60)
                                        CCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCG                                                       >  SRR4302540.437660/1‑94 (MQ=60)
                                        CCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCG                                                       <  SRR4302540.8460430/94‑1 (MQ=60)
                                         CAGATCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGT                                                      <  SRR4302540.2472688/94‑1 (MQ=60)
                                         CAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGT                                                      >  SRR4302540.6810629/1‑94 (MQ=60)
                                         CAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGT                                                      >  SRR4302540.7608113/1‑94 (MQ=60)
                                         CAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATAAGTCACACCGT                                                      >  SRR4302540.6810626/1‑94 (MQ=60)
                                           GAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAT                                                    <  SRR4302540.2640685/94‑1 (MQ=60)
                                            AGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGGCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATT                                                   >  SRR4302540.8571340/1‑94 (MQ=60)
                                            AGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATT                                                   >  SRR4302540.8571341/1‑94 (MQ=60)
                                             GCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTG                                                  >  SRR4302540.3279118/1‑94 (MQ=60)
                                              CATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGC                                                 <  SRR4302540.6944387/94‑1 (MQ=60)
                                              CATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGC                                                 <  SRR4302540.7947461/94‑1 (MQ=60)
                                               ATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCG                                                >  SRR4302540.444643/1‑94 (MQ=60)
                                               ATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCG                                                >  SRR4302540.9244744/1‑94 (MQ=60)
                                               ATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCG                                                >  SRR4302540.4818457/1‑94 (MQ=60)
                                                TCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGC                                               <  SRR4302540.4281162/94‑1 (MQ=60)
                                                  GCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCA                                             >  SRR4302540.5488078/1‑94 (MQ=60)
                                                  GCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGAATGAGTCACACCGTATTGCGCCA                                             <  SRR4302540.1858192/94‑1 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGT                                           >  SRR4302540.3426363/1‑94 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGT                                           >  SRR4302540.8862038/1‑94 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGT                                           >  SRR4302540.2741891/1‑94 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGT                                           >  SRR4302540.2083917/1‑94 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGT                                           <  SRR4302540.9837121/94‑1 (MQ=60)
                                                    GATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGACACACCGAATTGCGCCAGT                                           >  SRR4302540.8742204/1‑94 (MQ=60)
                                                     ATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTA                                          >  SRR4302540.3046223/1‑94 (MQ=60)
                                                      TACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAA                                         >  SRR4302540.2725967/1‑94 (MQ=60)
                                                       ACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAAT                                        >  SRR4302540.3380815/1‑94 (MQ=60)
                                                        CGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAGGAGTCACACCGTATTGCGCCAGTAATG                                       <  SRR4302540.4507499/94‑1 (MQ=60)
                                                         GTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGG                                      >  SRR4302540.2028211/1‑94 (MQ=60)
                                                         GTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGG                                      >  SRR4302540.8651951/1‑94 (MQ=60)
                                                          TTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGC                                     <  SRR4302540.3644224/94‑1 (MQ=60)
                                                          TTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGC                                     <  SRR4302540.6543223/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.7308091/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.7055998/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.7644190/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.735464/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.5282158/94‑1 (MQ=60)
                                                           TCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCA                                    <  SRR4302540.7779406/94‑1 (MQ=60)
                                                            CAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAG                                   <  SRR4302540.1626087/94‑1 (MQ=60)
                                                             AGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGC                                  >  SRR4302540.4779108/1‑94 (MQ=60)
                                                             AGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGC                                  <  SRR4302540.6373039/94‑1 (MQ=60)
                                                             AGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGC                                  >  SRR4302540.2154582/1‑94 (MQ=60)
                                                             AGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGC                                  >  SRR4302540.9777857/1‑94 (MQ=60)
                                                              GGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCG                                 <  SRR4302540.1344925/94‑1 (MQ=60)
                                                              GGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCG                                 <  SRR4302540.9545687/94‑1 (MQ=60)
                                                               GCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGA                                <  SRR4302540.2086957/94‑1 (MQ=60)
                                                                CTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAA                               >  SRR4302540.1841323/1‑94 (MQ=60)
                                                                 TGATCGCCCTGCTGGTTTGCCGGANTAGCGCCCGTCAGCGCATGACNGGGTTCGGCATGAGTCACACCGTNNNNCGNNAGTNATNGNNGNGNNN                              >  SRR4302540.8248523/1‑94 (MQ=60)
                                                                 TGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAG                              <  SRR4302540.2652512/94‑1 (MQ=60)
                                                                  GATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGT                             <  SRR4302540.4422295/94‑1 (MQ=60)
                                                                  GATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGT                             <  SRR4302540.3868002/94‑1 (MQ=60)
                                                                  GATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGT                             >  SRR4302540.7363996/1‑94 (MQ=60)
                                                                  GATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGG                             >  SRR4302540.766742/1‑94 (MQ=60)
                                                                   ATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTG                            <  SRR4302540.7284469/94‑1 (MQ=60)
                                                                   ATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTG                            <  SRR4302540.4142164/94‑1 (MQ=60)
                                                                   ATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGAG                            >  SRR4302540.4441526/1‑94 (MQ=60)
                                                                    TCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGC                           <  SRR4302540.7140388/94‑1 (MQ=60)
                                                                    TCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGC                           <  SRR4302540.5943006/94‑1 (MQ=60)
                                                                    TCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGC                           <  SRR4302540.6065147/94‑1 (MQ=60)
                                                                     CGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCA                          <  SRR4302540.4474299/94‑1 (MQ=60)
                                                                      GCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTTGGCATGAGTCACACCGTATTGCGCCCGTAATGGCAGCGAAGcgcac                         >  SRR4302540.5361654/1‑89 (MQ=60)
                                                                      GCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAG                         <  SRR4302540.5000397/94‑1 (MQ=60)
                                                                      GCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAG                         >  SRR4302540.5361653/1‑94 (MQ=60)
                                                                      GCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAG                         <  SRR4302540.4761848/94‑1 (MQ=60)
                                                                       CCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGC                        >  SRR4302540.5591738/1‑94 (MQ=60)
                                                                       CCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGC                        >  SRR4302540.5591739/1‑94 (MQ=60)
                                                                        CCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCT                       >  SRR4302540.4785239/1‑94 (MQ=60)
                                                                        CCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCT                       >  SRR4302540.2819281/1‑94 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAGGGGCAGCTG                      >  SRR4302540.6524752/1‑94 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTG                      <  SRR4302540.724992/94‑1 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTG                      <  SRR4302540.8539203/94‑1 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTG                      >  SRR4302540.5992435/1‑94 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTG                      >  SRR4302540.202577/1‑94 (MQ=60)
                                                                         CTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTG                      >  SRR4302540.5279063/1‑94 (MQ=60)
                                                                            CTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTC                   <  SRR4302540.1175014/94‑1 (MQ=60)
                                                                            CTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTC                   >  SRR4302540.9156735/1‑94 (MQ=60)
                                                                            CTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTC                   <  SRR4302540.6261703/94‑1 (MQ=60)
                                                                            CTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTC                   <  SRR4302540.5092408/94‑1 (MQ=60)
                                                                            CTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTC                   <  SRR4302540.4470781/94‑1 (MQ=60)
                                                                             TGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCG                  >  SRR4302540.5778871/1‑94 (MQ=60)
                                                                             TGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCG                  >  SRR4302540.4673536/1‑94 (MQ=60)
                                                                              GGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGC                 <  SRR4302540.894966/94‑1 (MQ=60)
                                                                               GTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCT                <  SRR4302540.826457/94‑1 (MQ=60)
                                                                               GTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCT                >  SRR4302540.3161938/1‑94 (MQ=60)
                                                                                TTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTG               <  SRR4302540.1609393/94‑1 (MQ=60)
                                                                                TTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTG               <  SRR4302540.6376925/94‑1 (MQ=60)
                                                                                 TTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGA              <  SRR4302540.1154957/94‑1 (MQ=60)
                                                                                 TTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGA              <  SRR4302540.3638960/94‑1 (MQ=60)
                                                                                  TGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAA             <  SRR4302540.3342698/94‑1 (MQ=60)
                                                                                  TGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAA             <  SRR4302540.4543859/94‑1 (MQ=60)
                                                                                   GCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAG            <  SRR4302540.5425032/94‑1 (MQ=60)
                                                                                   GCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAG            <  SRR4302540.3284571/94‑1 (MQ=60)
                                                                                   GCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAG            <  SRR4302540.2792421/94‑1 (MQ=60)
                                                                                   GCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAG            <  SRR4302540.9311356/94‑1 (MQ=60)
                                                                                    CCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGG           <  SRR4302540.3861708/94‑1 (MQ=60)
                                                                                    CCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGG           <  SRR4302540.1745498/94‑1 (MQ=60)
                                                                                    CCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGG           >  SRR4302540.8498855/1‑94 (MQ=60)
                                                                                    CCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGGGCAGCTGGTCGCTGGAGG           >  SRR4302540.8498857/1‑94 (MQ=60)
                                                                                     CGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGC          <  SRR4302540.1303938/94‑1 (MQ=60)
                                                                                     CGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGC          <  SRR4302540.1195797/94‑1 (MQ=60)
                                                                                     CGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGC          >  SRR4302540.9210787/1‑94 (MQ=60)
                                                                                      GGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCG         >  SRR4302540.5078612/1‑94 (MQ=60)
                                                                                        AATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGAGCG       >  SRR4302540.3512552/1‑94 (MQ=60)
                                                                                         ATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGT      >  SRR4302540.7952102/1‑94 (MQ=60)
                                                                                           AGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTC    <  SRR4302540.5361189/94‑1 (MQ=60)
                                                                                           AGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTC    >  SRR4302540.4415697/1‑94 (MQ=60)
                                                                                           AGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTC    <  SRR4302540.3382070/94‑1 (MQ=60)
                                                                                           AGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTC    >  SRR4302540.2087362/1‑94 (MQ=60)
                                                                                            GCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCA   <  SRR4302540.2921594/94‑1 (MQ=60)
                                                                                            GCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCA   <  SRR4302540.1719673/94‑1 (MQ=60)
                                                                                            GCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCA   <  SRR4302540.3261716/94‑1 (MQ=60)
                                                                                            GCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCA   <  SRR4302540.1799944/94‑1 (MQ=60)
                                                                                            GCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCA   <  SRR4302540.8586101/94‑1 (MQ=60)
                                                                                             CGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAG  >  SRR4302540.3107511/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGTAGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAG  >  NC_000913/3507738‑3507924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑