Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,4041.C99.8% 1501.5 / ‑4.6 468intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (308/159);  total (309/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
*NC_0009133,537,4042.G99.8% 1510.1 / ‑4.5 470intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (308/161);  total (309/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 505 total aligned reads displayed.

GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487
                                                                                             ||                                                                                     
 cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                                      <  2:65813/93‑1 (MQ=255)
 cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                                      <  2:2249545/93‑1 (MQ=255)
    tGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgcc                                                                                   >  1:3500905/1‑90 (MQ=255)
    tGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgcc                                                                                   >  1:8794720/1‑90 (MQ=255)
     ggcggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgccg                                                                                  >  1:8625154/1‑89 (MQ=255)
           cgcAATGGGAAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTg                                                                            <  1:3096325/92‑1 (MQ=37)
             cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGc                                                                          <  1:6689524/93‑1 (MQ=38)
             cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGc                                                                          <  1:7179072/93‑1 (MQ=37)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCa                                                                         <  1:1964352/93‑1 (MQ=37)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCa                                                                         >  1:2781773/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaa                                                                        >  1:9288324/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaa                                                                        >  2:6729284/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaa                                                                        >  2:5636941/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaa                                                                        <  2:1027029/93‑1 (MQ=37)
                tGGGCAATAAGCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGGAGATAAGGCGTTCACGC‑C‑GCATCTGGCaac                                                                       <  1:181598/93‑1 (MQ=25)
                tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaac                                                                       >  2:5941547/1‑93 (MQ=37)
                 gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacg                                                                      >  1:1614904/1‑93 (MQ=37)
                 gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacg                                                                      >  1:4972044/1‑93 (MQ=37)
                  ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacga                                                                     <  2:2028103/93‑1 (MQ=37)
                   gaaaaaacCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacgaa                                                                    <  2:9479574/91‑1 (MQ=37)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacgaa                                                                    <  2:4751791/93‑1 (MQ=37)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacgaa                                                                    <  2:7840954/93‑1 (MQ=37)
                     aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacgaacg                                                                  >  2:6000164/1‑93 (MQ=37)
                     aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCaacgaacg                                                                  >  2:9178930/1‑93 (MQ=37)
                      aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGc                                                                 >  2:8280290/1‑93 (MQ=37)
                       aaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGcc                                                                <  1:6117943/93‑1 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCt                                                               <  1:4590182/93‑1 (MQ=37)
                         aaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCtt                                                              >  1:2388111/1‑93 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGcc                                                           <  2:433591/93‑1 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGcc                                                           >  2:7115630/1‑93 (MQ=37)
                            gTTAAACGCCCGTACTGCCCTACGGTTCGAATTGGCACGAAATCGGAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGcc                                                           <  1:3890505/93‑1 (MQ=37)
                             ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCt                                                          <  1:474320/93‑1 (MQ=37)
                             ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCt                                                          <  2:9576094/93‑1 (MQ=37)
                               aaaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGa                                                        <  2:2887397/93‑1 (MQ=37)
                                aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGat                                                       >  1:398300/1‑92 (MQ=37)
                                aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGAc                                                       >  1:3427969/1‑93 (MQ=38)
                                aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGAc                                                       <  2:1400137/93‑1 (MQ=37)
                                    cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaa                                                   >  2:7426289/1‑93 (MQ=37)
                                     ccGTAATGGCCTACGGTTCGAATTTGCAGGAAATCGTATGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaaa                                                  <  2:3452725/93‑1 (MQ=37)
                                      cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                 >  1:9448312/1‑93 (MQ=37)
                                       gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                >  2:8085541/1‑93 (MQ=38)
                                          cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGGGc                                             >  1:7719891/1‑93 (MQ=37)
                                             gCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                                          <  2:8754129/93‑1 (MQ=37)
                                             gCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                                          >  1:4287387/1‑93 (MQ=37)
                                              cttACGGTTCGAATTGCCACGAAATGGTAGGTCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGga                                         <  2:4435146/91‑1 (MQ=21)
                                               cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                        >  1:8355014/1‑93 (MQ=38)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       >  1:6894551/1‑93 (MQ=38)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       >  2:7055178/1‑93 (MQ=37)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       >  2:1367792/1‑93 (MQ=38)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       >  1:2331251/1‑93 (MQ=38)
                                                  cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                     >  2:3004825/1‑93 (MQ=38)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                    >  1:595277/1‑93 (MQ=38)
                                                    gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                   >  1:117436/1‑93 (MQ=38)
                                                     ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                  <  1:5823650/93‑1 (MQ=37)
                                                     ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                  >  1:5602540/1‑93 (MQ=38)
                                                      tCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                 >  1:2108196/1‑93 (MQ=38)
                                                       cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtctc                                >  1:5157466/1‑93 (MQ=38)
                                                         aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg                              >  1:7982183/1‑93 (MQ=38)
                                                         aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAAATATCCg                              >  2:3771158/1‑93 (MQ=37)
                                                         aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CGCAGCATCTGGCAACGAACGACTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg                              >  1:5345994/1‑93 (MQ=37)
                                                          aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCgg                             <  2:3242719/93‑1 (MQ=38)
                                                           tttGCCCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            <  1:1522315/93‑1 (MQ=37)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  1:6261454/1‑93 (MQ=38)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  1:3750557/1‑93 (MQ=38)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  2:2628313/1‑93 (MQ=38)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  1:7536913/1‑93 (MQ=38)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  2:3926664/1‑93 (MQ=38)
                                                            ttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa                           >  2:7337010/1‑93 (MQ=38)
                                                              gCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt                         <  2:9287045/93‑1 (MQ=38)
                                                              gCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt                         <  1:8433675/93‑1 (MQ=38)
                                                              gCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt                         >  2:5173766/1‑93 (MQ=38)
                                                                aCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt                       >  1:2893838/1‑93 (MQ=38)
                                                                  gAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATCTCTCCAGCATTTTTa                     >  2:1936923/1‑93 (MQ=37)
                                                                  gAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTa                     >  1:1120476/1‑93 (MQ=38)
                                                                  gAAATCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTa                     >  1:3282805/1‑93 (MQ=38)
                                                                   aaaTGGTAGGGAAGATAGGCCTTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                    <  2:9026063/93‑1 (MQ=255)
                                                                   aaaTCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                    >  1:9205479/1‑93 (MQ=38)
                                                                   aaaTCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                    <  2:1642169/93‑1 (MQ=38)
                                                                    aaTCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAtt                   <  1:1310062/93‑1 (MQ=38)
                                                                     aTCGTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCCTTTTTATTc                  >  1:6584557/1‑93 (MQ=37)
                                                                        gTAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAGCGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcac               <  1:10004389/93‑1 (MQ=37)
                                                                         tAGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca              >  1:5511055/1‑93 (MQ=38)
                                                                          aGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg             <  2:610348/93‑1 (MQ=38)
                                                                          aGGGCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg             >  1:7446586/1‑93 (MQ=38)
                                                                           gggCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCACAGc            >  2:111863/1‑93 (MQ=38)
                                                                            ggCAGATAAGGCGTGCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGcc           <  1:6036668/93‑1 (MQ=38)
                                                                             gCAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa          >  2:6466043/1‑93 (MQ=38)
                                                                              cAGATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaa         >  1:9949577/1‑93 (MQ=38)
                                                                                gATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTTCCTGACATAAAAGTGCCGGCGAATATCTCCGGCATTTTTTTTCCACAGCCAAAc       >  1:8255174/1‑93 (MQ=37)
                                                                                gATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGGATATCTACGGCCTTTTTTTTTTACAGCCCAAc       >  2:5423822/1‑93 (MQ=25)
                                                                                gATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAc       >  2:759996/1‑93 (MQ=39)
                                                                                gATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAc       >  1:2553865/1‑93 (MQ=39)
                                                                                gATAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTGTTATTCCACAGCCAAAc       >  1:8979334/1‑93 (MQ=38)
                                                                                 aTAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGGCGTGCCTGACATAAAAGTGCCGGAGACTATCTCCGGCATTTTTATTCCACAGCCAAACt      <  2:8341258/93‑1 (MQ=37)
                                                                                 aTAAGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACt      >  2:9723404/1‑93 (MQ=39)
                                                                                   aaGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCa    >  2:6273807/1‑93 (MQ=39)
                                                                                   aaGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCa    >  2:5765830/1‑93 (MQ=39)
                                                                                    aGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCat   <  1:9637279/93‑1 (MQ=39)
                                                                                    aGGCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCat   >  2:4927942/1‑93 (MQ=255)
                                                                                     ggCGTTCACGC‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCata  >  1:4489527/1‑93 (MQ=39)
                                                                                             ||                                                                                     
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 325 total aligned reads displayed.
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498
                                                                                               |                                                                                                
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                                  <  SRR4302540.7930725/94‑1 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgc                                                                                                >  SRR4302540.2636148/1‑92 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgc                                                                                                >  SRR4302540.2231557/1‑92 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgc                                                                                                <  SRR4302540.1529/94‑3 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                               <  SRR4302540.1161033/94‑4 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                               >  SRR4302540.1386226/1‑91 (MQ=60)
     GGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgc                                                                                             >  SRR4302540.579820/1‑89 (MQ=60)
     GGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgc                                                                                             >  SRR4302540.5718668/1‑89 (MQ=60)
      GCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑A                                                                                           >  SRR4302540.4173540/1‑94 (MQ=60)
        GGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATC                                                                                         >  SRR4302540.6926804/1‑94 (MQ=60)
           GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGG                                                                                      >  SRR4302540.8188457/1‑94 (MQ=60)
           GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATATGG                                                                                      >  SRR4302540.8151588/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCA                                                                                    <  SRR4302540.6763383/94‑1 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCA                                                                                    <  SRR4302540.7259102/94‑1 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCA                                                                                    >  SRR4302540.2811824/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAAC                                                                                  >  SRR4302540.5965406/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAAC                                                                                  >  SRR4302540.5906349/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAAC                                                                                  <  SRR4302540.3929507/94‑1 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAAC                                                                                  >  SRR4302540.8411198/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAAC                                                                                  >  SRR4302540.9726391/1‑94 (MQ=60)
                TGGGCAATAAGCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGGAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACG                                                                                 <  SRR4302540.183405/94‑1 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACG                                                                                 >  SRR4302540.1632622/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACG                                                                                 <  SRR4302540.183404/94‑1 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACG                                                                                 >  SRR4302540.5025844/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGCAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACG                                                                                 >  SRR4302540.2206295/1‑94 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAA                                                                               <  SRR4302540.5587477/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAAC                                                                              >  SRR4302540.5270061/1‑94 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAAC                                                                              >  SRR4302540.3590472/1‑94 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAAC                                                                              <  SRR4302540.3187510/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAAC                                                                              >  SRR4302540.9288343/1‑94 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACG                                                                             >  SRR4302540.4452511/1‑94 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGC                                                                            >  SRR4302540.10024161/1‑94 (MQ=60)
                      AAAAGCGTTAAACGCCCGTACTGGCCTACGTTTCGAATTTGCACGAATTCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCC                                                                           <  SRR4302540.6006544/94‑1 (MQ=60)
                      AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCC                                                                           <  SRR4302540.10139393/94‑1 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCT                                                                          >  SRR4302540.6294868/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCT                                                                          <  SRR4302540.6186075/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTT                                                                         >  SRR4302540.873494/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTT                                                                         <  SRR4302540.4639597/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTT                                                                         >  SRR4302540.2413852/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTT                                                                         <  SRR4302540.4639596/94‑1 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTG                                                                        >  SRR4302540.8304539/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTG                                                                        <  SRR4302540.9538813/94‑1 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTG                                                                        >  SRR4302540.9562522/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTG                                                                        <  SRR4302540.1186424/94‑1 (MQ=60)
                          ACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGC                                                                       <  SRR4302540.9341967/94‑1 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCC                                                                      >  SRR4302540.4610096/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302540.2384132/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     <  SRR4302540.3932746/94‑1 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302540.8646183/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302540.9304921/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302540.5000791/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGCTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302540.2062363/1‑94 (MQ=60)
                             TTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTG                                                                    <  SRR4302540.479399/94‑1 (MQ=60)
                             TTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTG                                                                    >  SRR4302540.6697181/1‑94 (MQ=60)
                             TTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTG                                                                    >  SRR4302540.5881202/1‑94 (MQ=60)
                              TAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGA                                                                   >  SRR4302540.9612048/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGAT                                                                  >  SRR4302540.402430/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302540.1142687/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302540.3465223/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302540.1984188/1‑94 (MQ=60)
                                AACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACA                                                                 >  SRR4302540.3028770/1‑94 (MQ=60)
                                AACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACA                                                                 >  SRR4302540.1201731/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACAT                                                                <  SRR4302540.5235925/94‑1 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAA                                                              <  SRR4302540.135140/94‑1 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAA                                                              >  SRR4302540.1078437/1‑94 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAA                                                              <  SRR4302540.6449183/94‑1 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAA                                                              <  SRR4302540.6095552/94‑1 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             >  SRR4302540.10038447/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATACAA                                                            >  SRR4302540.7791280/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302540.6887168/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302540.10107678/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                           >  SRR4302540.9017404/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          <  SRR4302540.3232002/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302540.6162000/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          <  SRR4302540.3246165/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302540.7516142/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          <  SRR4302540.6237976/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGG                                                          >  SRR4302540.6162001/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCCGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          <  SRR4302540.285458/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGAAAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302540.5263513/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302540.3943137/94‑1 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302540.2366750/94‑1 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGAGAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302540.1631602/94‑1 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                        >  SRR4302540.7651258/1‑94 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                        >  SRR4302540.7651257/1‑94 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGGGC                                                        >  SRR4302540.7809358/1‑94 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAACCGTAGGGCAGCTAAGGCGTTCACGCCGC‑‑‑ACATGGCAACGAACGCCTTGCCTGACATAAAAGCGC                                                        >  SRR4302540.3337382/1‑94 (MQ=60)
                                           TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                      >  SRR4302540.5439176/1‑94 (MQ=60)
                                           TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                      >  SRR4302540.3991350/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTTCCTGACATAAAAGTGCCCG                                                     >  SRR4302540.466129/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     >  SRR4302540.5494023/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     >  SRR4302540.8718572/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     >  SRR4302540.9559688/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302540.8451984/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302540.8901612/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAGGTGCCGGAGA                                                  >  SRR4302540.7498348/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302540.6923165/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302540.2356368/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302540.7062541/1‑94 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                <  SRR4302540.606361/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302540.5158394/1‑94 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302540.5469277/1‑94 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              >  SRR4302540.8472479/1‑94 (MQ=60)
                                                   GGTTCGAATGTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              <  SRR4302540.2780441/94‑1 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302540.5664190/1‑94 (MQ=60)
                                                     TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            <  SRR4302540.8707762/94‑1 (MQ=60)
                                                     TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            <  SRR4302540.5888025/94‑1 (MQ=60)
                                                     TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            >  SRR4302540.2130822/1‑94 (MQ=60)
                                                     TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            >  SRR4302540.2492491/1‑94 (MQ=60)
                                                      TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           >  SRR4302540.5213477/1‑94 (MQ=60)
                                                      TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           <  SRR4302540.8444627/94‑1 (MQ=60)
                                                       CGAATTTGCAGAAAATCGTAGGGCAGATAAGGCGTACACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          <  SRR4302540.3984045/94‑1 (MQ=60)
                                                       CGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          >  SRR4302540.1921501/1‑94 (MQ=60)
                                                       CGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          >  SRR4302540.8471413/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302540.6701996/94‑1 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         >  SRR4302540.7583619/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302540.1586626/94‑1 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         >  SRR4302540.8075146/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         >  SRR4302540.3632012/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         >  SRR4302540.8075148/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302540.719349/94‑1 (MQ=60)
                                                         AATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        >  SRR4302540.6199654/1‑94 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302540.9333937/1‑94 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302540.3791477/1‑94 (MQ=60)
                                                           TTTGCCCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCA                                      <  SRR4302540.1539162/94‑1 (MQ=60)
                                                           TTCGCACGAAATCGTAGGGCAGATAAGGGGTGCA‑‑‑CGCAGTATCTGGCAACGAACGCCTTGCCCGACATAAAAGTGCCGGAGAATATCTCCGGCA                                      <  SRR4302540.6967056/94‑1 (MQ=60)
                                                            TTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAT                                     <  SRR4302540.4451065/94‑1 (MQ=60)
                                                            TTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATATCCGGCAT                                     >  SRR4302540.2079845/1‑94 (MQ=60)
                                                             TGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                    <  SRR4302540.3341301/94‑1 (MQ=60)
                                                              GCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTT                                   <  SRR4302540.8531584/94‑1 (MQ=60)
                                                               CACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                  >  SRR4302540.2925391/1‑94 (MQ=60)
                                                               CACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCCGCCTTTT                                  >  SRR4302540.2109771/1‑94 (MQ=60)
                                                                tcaaaaccgtagtgaAGATAAGGCATTCACGCCGC‑‑‑ATCTGTCATCGAACGCTTTGCTTGCCATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 <  SRR4302540.10002799/79‑1 (MQ=31)
                                                                ACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 >  SRR4302540.5156526/1‑94 (MQ=60)
                                                                 CGAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                                >  SRR4302540.1132103/1‑94 (MQ=60)
                                                                  GAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                               <  SRR4302540.9452086/94‑1 (MQ=60)
                                                                  GAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                               >  SRR4302540.4042032/1‑94 (MQ=60)
                                                                  GAAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                               >  SRR4302540.9315937/1‑94 (MQ=60)
                                                                   AAATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                              <  SRR4302540.9395786/94‑1 (MQ=60)
                                                                    AATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCCTTTTTATTC                             >  SRR4302540.6657491/1‑94 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            >  SRR4302540.4934802/1‑94 (MQ=60)
                                                                      TCGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCA                           >  SRR4302540.1758960/1‑94 (MQ=60)
                                                                      TCGGAGGGCAAATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCA                           <  SRR4302540.3866938/94‑1 (MQ=60)
                                                                       CGTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                          >  SRR4302540.7585382/1‑94 (MQ=60)
                                                                        GTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302540.3742230/94‑1 (MQ=60)
                                                                        GTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302540.3499650/94‑1 (MQ=60)
                                                                        GTAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         >  SRR4302540.5571755/1‑94 (MQ=60)
                                                                         TAGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAC                        >  SRR4302540.2897006/1‑94 (MQ=60)
                                                                          AGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       >  SRR4302540.6528319/1‑94 (MQ=60)
                                                                          AGGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       <  SRR4302540.1245003/94‑1 (MQ=60)
                                                                           GGGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCC                      >  SRR4302540.6582328/1‑94 (MQ=60)
                                                                            GGCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                     >  SRR4302540.5631806/1‑94 (MQ=60)
                                                                             GCAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                    >  SRR4302540.10065741/1‑94 (MQ=60)
                                                                              CAGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                   >  SRR4302540.8840900/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTTCCTGACATAAAAGTGCCGGCGAATATCTCCGGCATTTTTTTTCCACAGCCAAAC                  >  SRR4302540.8351120/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302540.3391715/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302540.2581384/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302540.6901707/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  <  SRR4302540.5318929/94‑1 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTGTTATTCCACAGCCAAAC                  >  SRR4302540.9086302/1‑94 (MQ=60)
                                                                                GATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACT                 >  SRR4302540.6094596/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                >  SRR4302540.10157786/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                >  SRR4302540.4543815/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                >  SRR4302540.7618630/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                >  SRR4302540.8892843/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                <  SRR4302540.2524064/94‑1 (MQ=60)
                                                                                  TAAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCA               <  SRR4302540.3533049/94‑1 (MQ=60)
                                                                                   AAGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCAT              <  SRR4302540.3013547/94‑1 (MQ=60)
                                                                                   AAGGCGTCCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCAT              <  SRR4302540.7541176/94‑1 (MQ=60)
                                                                                    AGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             <  SRR4302540.9750816/94‑1 (MQ=60)
                                                                                    AGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302540.4278500/1‑94 (MQ=60)
                                                                                    AGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302540.1243994/1‑94 (MQ=60)
                                                                                    AGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302540.4538213/1‑94 (MQ=60)
                                                                                    AGGCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302540.7263639/1‑94 (MQ=60)
                                                                                      GCGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT           >  SRR4302540.2848023/1‑94 (MQ=60)
                                                                                       ggttcacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATACCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA          <  SRR4302540.9577289/86‑1 (MQ=60)
                                                                                       ggtgcactCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCAGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA          <  SRR4302540.1524347/86‑1 (MQ=60)
                                                                                       CGTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA          <  SRR4302540.8145099/94‑1 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCACAGCCAAACTCATAATAT         >  SRR4302540.8970706/1‑94 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302540.9948062/1‑94 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         <  SRR4302540.8532560/94‑1 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302540.8424871/1‑94 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302540.4294361/1‑94 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302540.5241303/1‑94 (MQ=60)
                                                                                        GTTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAGTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302540.4825189/1‑94 (MQ=60)
                                                                                         ttctcgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA        <  SRR4302540.2262991/88‑1 (MQ=60)
                                                                                         TTCACGCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA        <  SRR4302540.3056440/94‑1 (MQ=60)
                                                                                          tcacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       >  SRR4302540.9428277/6‑94 (MQ=60)
                                                                                          tcacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       <  SRR4302540.4874465/89‑1 (MQ=60)
                                                                                          tcacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       >  SRR4302540.7084295/6‑94 (MQ=60)
                                                                                           cacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      >  SRR4302540.2847152/5‑94 (MQ=60)
                                                                                           cacgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      >  SRR4302540.2770232/5‑94 (MQ=60)
                                                                                            acgCCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC     <  SRR4302540.5791092/91‑1 (MQ=60)
                                                                                            G‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGGGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302540.5088186/1‑94 (MQ=60)
                                                                                            G‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGCATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302540.4840648/1‑94 (MQ=60)
                                                                                            G‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302540.9418453/1‑94 (MQ=60)
                                                                                            G‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   <  SRR4302540.4396016/94‑1 (MQ=60)
                                                                                            G‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302540.6674848/1‑94 (MQ=60)
                                                                                               CCGC‑‑‑ATCTGGCAACGAACGCCTTTCCTGACATAAAATTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  SRR4302540.1849474/1‑94 (MQ=60)
                                                                                               CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCAGGAGGAAATATCCGGTATTTTTATTTCACAACCAAAATCACAATATATTCTGG  >  SRR4302540.9007876/1‑94 (MQ=46)
                                                                                               |                                                                                                
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGC‑‑‑ATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑