Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,544,360 +G coding (166/1314 nt) gntT → gluconate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,3581.G99.1% 1007.6 / 0.3 317coding (164/1314 nt)gntTgluconate permease
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (172/141);  total (174/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 329 total aligned reads displayed.

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑C‑GC‑G‑GA‑C‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437
                                                                              |                                                                                   
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  1:7756510/1‑93 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  1:1723171/1‑93 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:2986378/1‑93 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:6639203/1‑93 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGcc                                                                   >  2:2247569/1‑93 (MQ=255)
  gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCtt                                                                 >  2:2142673/1‑93 (MQ=255)
  gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCtt                                                                 >  1:4448862/1‑93 (MQ=255)
   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTg                                                                >  2:3433469/1‑93 (MQ=255)
   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTg                                                                >  2:8728093/1‑93 (MQ=255)
   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGGGT‑CGGCGG‑GA‑A‑GCTCGGTAGCCTTg                                                                >  2:3329686/1‑93 (MQ=255)
    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               >  2:8083189/1‑93 (MQ=255)
    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               <  2:251590/93‑1 (MQ=255)
    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCAG‑GA‑C‑GCTCGGTAGCCTTGc                                                               <  1:2024660/93‑1 (MQ=255)
    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGACTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGc                                                               >  1:7165725/1‑93 (MQ=255)
      cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGccc                                                             <  2:6610296/93‑1 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTAG‑GCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           <  1:3822436/93‑1 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           >  2:7878001/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           >  1:3596692/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTg                                                           <  2:5676841/93‑1 (MQ=255)
         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGa                                                          >  2:8035072/1‑93 (MQ=255)
          tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGAt                                                         >  2:8971200/1‑93 (MQ=255)
           gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATc                                                        >  1:8578041/1‑93 (MQ=255)
            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:6651754/93‑1 (MQ=255)
            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:798851/93‑1 (MQ=255)
            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:2319639/93‑1 (MQ=255)
            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  2:4668475/93‑1 (MQ=255)
            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  1:5021046/93‑1 (MQ=255)
            cTGTGGGATTAATGNAAGGAATGCCGCTGGATNAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  2:4800907/93‑1 (MQ=255)
             tGTTGGATTAATGGAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:87459/93‑1 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:420990/1‑93 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:9456314/1‑93 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:124336/1‑93 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:2006975/1‑93 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCAt                                                      <  2:88426/93‑1 (MQ=255)
                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   >  1:2933993/1‑93 (MQ=255)
                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   >  1:9066944/1‑93 (MQ=255)
                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATggg                                                   <  1:478437/93‑1 (MQ=255)
                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GACC‑C‑TCGGTAGTCTTTCCCTGATCATGGGt                                                  >  2:7629193/1‑93 (MQ=255)
                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GACC‑C‑TCGGTCGCCTTGCCCTGATCCTgggat                                                 >  2:7239022/1‑91 (MQ=255)
                   aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:5299792/93‑1 (MQ=255)
                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               <  1:1891225/93‑1 (MQ=255)
                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGgttt                                               >  1:8938397/1‑93 (MQ=255)
                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:7349373/1‑93 (MQ=255)
                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  2:6352859/93‑1 (MQ=255)
                         gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                          >  1:637206/1‑93 (MQ=255)
                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:4966572/1‑93 (MQ=255)
                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  1:1525466/1‑93 (MQ=255)
                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         <  2:5935039/93‑1 (MQ=255)
                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  1:9610833/1‑93 (MQ=255)
                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGGCCTGATCATGGGTTTTGGCGCaa                                        >  2:9174620/1‑93 (MQ=255)
                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:3464643/93‑1 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑CGG‑TCGGTAGCCTTGCCGTGATCATGGGTTGTGGCGCAAt                                       >  1:6953023/1‑93 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaag                                       >  2:3597467/1‑92 (MQ=255)
                             ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:2912543/1‑93 (MQ=255)
                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:7425982/1‑93 (MQ=255)
                               aaTGCCGCTGGATAAAGTTGTTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  2:7432013/93‑1 (MQ=255)
                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:4557897/93‑1 (MQ=255)
                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    >  1:640987/1‑93 (MQ=255)
                                aTGCCGCTTGATAAATTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATTGGTTTTGGCGCAATGCTg                                   <  2:9562946/93‑1 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  2:8633999/1‑93 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:9285887/93‑1 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:5943890/1‑93 (MQ=255)
                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  2:1450636/1‑93 (MQ=255)
                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTTC‑CGGCGG‑GA‑C‑GCTCCGTAGCCCTGCCCTCATCCTGGGTTTTGGCGCAATGCTGGGc                                >  1:2460835/1‑93 (MQ=255)
                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                >  1:8355596/1‑93 (MQ=255)
                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:947837/93‑1 (MQ=255)
                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:7641254/1‑93 (MQ=255)
                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:2642059/1‑93 (MQ=255)
                                      cTGTATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             <  1:2843697/93‑1 (MQ=255)
                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAAt                           >  2:3926058/1‑93 (MQ=255)
                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGGAGCCTTGCCCTGATCATGGGCTTTGGCGCAATACTGGGCAAAAt                           >  1:8243782/1‑93 (MQ=255)
                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  1:9684231/1‑93 (MQ=255)
                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  1:1192418/93‑1 (MQ=255)
                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  1:6910535/1‑93 (MQ=255)
                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:2646725/1‑93 (MQ=255)
                                            aaaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:4514132/1‑93 (MQ=255)
                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:4168766/1‑93 (MQ=255)
                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:6759319/93‑1 (MQ=255)
                                             aaGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:3911895/1‑93 (MQ=255)
                                              aGTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  1:1373846/93‑1 (MQ=255)
                                               gTTATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    <  2:668085/93‑1 (MQ=255)
                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  2:1880445/1‑93 (MQ=255)
                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   <  1:6007005/93‑1 (MQ=255)
                                                ttATTGGCTCCATCAAAGCCGGTGT‑CGGC‑GTGA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGATTTGGCGTAATGCTGGGCAAAATGCTGGCAg                   >  2:5518271/1‑93 (MQ=255)
                                                 tATTGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  <  1:8112150/93‑1 (MQ=255)
                                                   ttGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:1272632/1‑93 (MQ=255)
                                                   ttGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:3135707/1‑93 (MQ=255)
                                                    tGGCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  2:7088012/1‑93 (MQ=255)
                                                     ggCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:2200740/93‑1 (MQ=255)
                                                      gCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  2:3938379/93‑1 (MQ=255)
                                                      gCTCCATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  2:1926344/93‑1 (MQ=255)
                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  2:8482999/1‑93 (MQ=255)
                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  1:8048091/1‑93 (MQ=255)
                                                          cATCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  2:9763588/93‑1 (MQ=255)
                                                           aTCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  1:9835737/1‑93 (MQ=255)
                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:5760960/1‑93 (MQ=255)
                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:5235587/1‑93 (MQ=255)
                                                            tCAAAGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:4723820/1‑93 (MQ=255)
                                                                aGCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:5782566/1‑93 (MQ=255)
                                                                 gCCGGTGT‑CGGCGG‑GA‑C‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:9176815/93‑1 (MQ=255)
                                                                              |                                                                                   
TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGT‑C‑GC‑G‑GA‑C‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑G‑‑CGGAC‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCAC  >  NC_000913/3544268‑3544451
                                                                                                     |                                                                                             
TCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑CGG‑‑CGG                                                                                               <  SRR4302540.2867886/94‑1 (MQ=60)
               TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCT                                                                                <  SRR4302540.1990208/94‑1 (MQ=60)
                CGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTT                                                                               <  SRR4302540.8871345/94‑1 (MQ=60)
                CGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTT                                                                               <  SRR4302540.4804219/94‑1 (MQ=60)
                CGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTT                                                                               >  SRR4302540.4497080/1‑94 (MQ=60)
                CGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTT                                                                               >  SRR4302540.2932768/1‑94 (MQ=60)
                CGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTT                                                                               <  SRR4302540.8793750/94‑1 (MQ=60)
                 GTGTCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTG                                                                              <  SRR4302540.3623823/94‑1 (MQ=60)
                 GTGGTGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTG                                                                              >  SRR4302540.5168188/1‑94 (MQ=60)
                  TGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGC                                                                             <  SRR4302540.4603003/94‑1 (MQ=60)
                  TGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGACTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGC                                                                             >  SRR4302540.7245614/1‑94 (MQ=60)
                   GGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCC                                                                            <  SRR4302540.5051686/94‑1 (MQ=60)
                   GGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCAGGAC‑GCTCGGTAGCCTTGCC                                                                            <  SRR4302540.2046535/94‑1 (MQ=60)
                    GCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCC                                                                           >  SRR4302540.7015149/1‑94 (MQ=60)
                     CGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCT                                                                          <  SRR4302540.1597620/94‑1 (MQ=60)
                      GCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTG                                                                         >  SRR4302540.3635992/1‑94 (MQ=60)
                      GCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTG                                                                         <  SRR4302540.3126702/94‑1 (MQ=60)
                      GCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTG                                                                         <  SRR4302540.8318454/94‑1 (MQ=60)
                       CTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GTAGGC‑G‑‑‑GGAC‑GCTCGGTAGCCTTGCCCTGA                                                                        <  SRR4302540.3864039/94‑1 (MQ=60)
                       CTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGA                                                                        >  SRR4302540.1696198/1‑94 (MQ=60)
                        TTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGAT                                                                       >  SRR4302540.8728129/1‑94 (MQ=60)
                        TTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGAT                                                                       <  SRR4302540.6457389/94‑1 (MQ=60)
                        TTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGAT                                                                       <  SRR4302540.6457388/94‑1 (MQ=60)
                         TGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATC                                                                      >  SRR4302540.8677745/1‑94 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.6725331/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    >  SRR4302540.4098563/1‑94 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.807333/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.1465716/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.5075401/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    >  SRR4302540.9568534/1‑94 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    >  SRR4302540.5760740/1‑94 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.2344611/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.6769099/94‑1 (MQ=60)
                           CTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCAT                                                                    <  SRR4302540.6590755/94‑1 (MQ=60)
                            TGTTGGATTAATGGAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATG                                                                   <  SRR4302540.88386/94‑1 (MQ=60)
                            TGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATG                                                                   >  SRR4302540.1625412/1‑94 (MQ=60)
                            TGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATG                                                                   >  SRR4302540.9274830/1‑94 (MQ=60)
                            TGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATG                                                                   >  SRR4302540.6556466/1‑94 (MQ=60)
                             GTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGG                                                                  <  SRR4302540.8090235/94‑1 (MQ=60)
                             GTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGG                                                                  <  SRR4302540.1992059/94‑1 (MQ=60)
                              TTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGG                                                                 >  SRR4302540.9175817/1‑94 (MQ=60)
                              TTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGG                                                                 >  SRR4302540.2966188/1‑94 (MQ=60)
                               TGGATTAATGCGAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGT                                                                >  SRR4302540.6083032/1‑94 (MQ=60)
                               TGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGT                                                                >  SRR4302540.9286208/1‑94 (MQ=60)
                               TGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGT                                                                <  SRR4302540.483561/94‑1 (MQ=60)
                                 GATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTT                                                              <  SRR4302540.2707221/94‑1 (MQ=60)
                                  GTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTT                                                             <  SRR4302540.7521249/94‑1 (MQ=60)
                                  ATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTT                                                             <  SRR4302540.9908699/94‑1 (MQ=60)
                                  ATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTT                                                             <  SRR4302540.3790595/94‑1 (MQ=60)
                                  ATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTT                                                             <  SRR4302540.8371025/94‑1 (MQ=60)
                                  ATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTT                                                             >  SRR4302540.9043166/1‑94 (MQ=60)
                                   TTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTG                                                            >  SRR4302540.4387773/1‑94 (MQ=60)
                                   TTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTG                                                            <  SRR4302540.4076491/94‑1 (MQ=60)
                                   TTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTG                                                            <  SRR4302540.1911929/94‑1 (MQ=60)
                                   TTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTG                                                            <  SRR4302540.2732871/94‑1 (MQ=60)
                                   TTAATGCAAGGAATGCAGATGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCCGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTG                                                            <  SRR4302540.6028359/94‑1 (MQ=60)
                                    TAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGG                                                           >  SRR4302540.228989/1‑94 (MQ=60)
                                    TAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGG                                                           >  SRR4302540.1257473/1‑94 (MQ=60)
                                     AATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGC                                                          >  SRR4302540.7868193/1‑94 (MQ=60)
                                     AATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGC                                                          >  SRR4302540.7431365/1‑94 (MQ=60)
                                      ATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCG                                                         >  SRR4302540.9006144/1‑94 (MQ=60)
                                      ATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCG                                                         >  SRR4302540.4791960/1‑94 (MQ=60)
                                       TGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGC                                                        <  SRR4302540.830235/94‑1 (MQ=60)
                                       TGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGC                                                        >  SRR4302540.643983/1‑94 (MQ=60)
                                       TGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCCC                                                        >  SRR4302540.9921965/1‑94 (MQ=60)
                                        GCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCA                                                       >  SRR4302540.9724153/1‑94 (MQ=60)
                                        GCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCA                                                       >  SRR4302540.1542360/1‑94 (MQ=60)
                                        ACAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCA                                                       <  SRR4302540.3923535/94‑1 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAA                                                      >  SRR4302540.6073037/1‑94 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAA                                                      >  SRR4302540.2963808/1‑94 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAA                                                      >  SRR4302540.8663004/1‑94 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAA                                                      >  SRR4302540.775210/1‑94 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAA                                                      <  SRR4302540.2088177/94‑1 (MQ=60)
                                         CAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGACTTGCCCTGATCATGGGTTTTGGCGCAA                                                      >  SRR4302540.8997102/1‑94 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGTGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAT                                                     >  SRR4302540.4642090/1‑94 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGACGG‑TCGGTAGCCTTGCCGTGATCATGGGTTGTGGCGCAAT                                                     >  SRR4302540.7030381/1‑94 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAT                                                     <  SRR4302540.3502353/94‑1 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAT                                                     >  SRR4302540.3863126/1‑94 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAT                                                     >  SRR4302540.2535109/1‑94 (MQ=60)
                                          AAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAT                                                     <  SRR4302540.8611594/94‑1 (MQ=60)
                                           AGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATG                                                    <  SRR4302540.7742515/94‑1 (MQ=60)
                                           AGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATG                                                    <  SRR4302540.8144230/94‑1 (MQ=60)
                                           AGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATG                                                    <  SRR4302540.2841225/94‑1 (MQ=60)
                                           AGAAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATG                                                    <  SRR4302540.7956325/94‑1 (MQ=60)
                                            GGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGC                                                   >  SRR4302540.7508704/1‑94 (MQ=60)
                                            GGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGC                                                   >  SRR4302540.7508706/1‑94 (MQ=60)
                                             GAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCT                                                  >  SRR4302540.647816/1‑94 (MQ=60)
                                              AATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTG                                                 <  SRR4302540.4607163/94‑1 (MQ=60)
                                              AATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTG                                                 <  SRR4302540.5953160/94‑1 (MQ=60)
                                              AATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTG                                                 >  SRR4302540.6009597/1‑94 (MQ=60)
                                              AATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTG                                                 >  SRR4302540.6009598/1‑94 (MQ=60)
                                              AATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTG                                                 <  SRR4302540.9134190/94‑1 (MQ=60)
                                               ATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGG                                                >  SRR4302540.7061179/1‑94 (MQ=60)
                                               ATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGG                                                <  SRR4302540.7301661/94‑1 (MQ=60)
                                               ATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGG                                                >  SRR4302540.3613634/1‑94 (MQ=60)
                                               ATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGG                                                >  SRR4302540.10055989/1‑94 (MQ=60)
                                                TGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGG                                               <  SRR4302540.301637/94‑1 (MQ=60)
                                                 GCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTTCCGG‑‑‑C‑G‑‑‑GGAC‑GCTCCGTAGCCCTGCCCTCATCCTGGGTTTTGGCGCAATGCTGGGC                                              >  SRR4302540.2487325/1‑94 (MQ=38)
                                                 GCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGC                                              <  SRR4302540.1365062/94‑1 (MQ=60)
                                                 GCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGC                                              >  SRR4302540.8452573/1‑94 (MQ=60)
                                                  CCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCA                                             <  SRR4302540.7493880/94‑1 (MQ=60)
                                                  CCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCACAATGCTGGGCA                                             >  SRR4302540.871655/1‑94 (MQ=60)
                                                   CGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCCA                                            >  SRR4302540.2658797/1‑94 (MQ=60)
                                                   CGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAA                                            >  SRR4302540.7381858/1‑94 (MQ=60)
                                                   CGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAA                                            >  SRR4302540.7727249/1‑94 (MQ=60)
                                                   CGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAA                                            >  SRR4302540.2670709/1‑94 (MQ=60)
                                                    GCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAA                                           <  SRR4302540.4731402/94‑1 (MQ=60)
                                                     CTGTATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAA                                          <  SRR4302540.2874468/94‑1 (MQ=60)
                                                     CTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAA                                          >  SRR4302540.4601256/1‑94 (MQ=60)
                                                      TGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAT                                         <  SRR4302540.7973763/94‑1 (MQ=60)
                                                      TGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAT                                         <  SRR4302540.1209837/94‑1 (MQ=60)
                                                      TGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGGAGCCTTGCCCTGATCATGGGCTTTGGCGCAATACTGGGCAAAAT                                         >  SRR4302540.8339595/1‑94 (MQ=60)
                                                       GGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATG                                        >  SRR4302540.1351206/1‑94 (MQ=60)
                                                       GGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATG                                        >  SRR4302540.6383117/1‑94 (MQ=60)
                                                       GGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATG                                        <  SRR4302540.9755727/94‑1 (MQ=60)
                                                       GGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATG                                        <  SRR4302540.338349/94‑1 (MQ=60)
                                                         ATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCT                                      >  SRR4302540.9798166/1‑94 (MQ=60)
                                                          TAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GTTGGC‑G‑‑‑GGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGTTCGGCAAGATGCTG                                     >  SRR4302540.8370509/1‑94 (MQ=60)
                                                          TAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTG                                     <  SRR4302540.1204707/94‑1 (MQ=60)
                                                          TAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTG                                     >  SRR4302540.6987415/1‑94 (MQ=60)
                                                          TAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTG                                     >  SRR4302540.8370506/1‑94 (MQ=60)
                                                          TAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTG                                     >  SRR4302540.4756893/1‑94 (MQ=60)
                                                           AAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGG                                    >  SRR4302540.9092379/1‑94 (MQ=60)
                                                           AAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGG                                    >  SRR4302540.9493114/1‑94 (MQ=60)
                                                           AAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGG                                    >  SRR4302540.4213975/1‑94 (MQ=60)
                                                           AAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGG                                    >  SRR4302540.3954277/1‑94 (MQ=60)
                                                            AAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGC                                   <  SRR4302540.7704997/94‑1 (MQ=60)
                                                             AGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCA                                  <  SRR4302540.1388416/94‑1 (MQ=60)
                                                             AGGTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCA                                  <  SRR4302540.5033618/94‑1 (MQ=60)
                                                              GTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAG                                 >  SRR4302540.7092910/1‑94 (MQ=60)
                                                              GTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAG                                 <  SRR4302540.9613217/94‑1 (MQ=60)
                                                              GTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGCTTTGGCGCAATGCTGGGCAAAATGCTGGCAG                                 >  SRR4302540.3228089/1‑94 (MQ=60)
                                                               TTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCACAATGCTGGCAGA                                >  SRR4302540.1533717/1‑94 (MQ=60)
                                                               TTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA                                <  SRR4302540.9574282/94‑1 (MQ=60)
                                                               TTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA                                <  SRR4302540.6073606/94‑1 (MQ=60)
                                                                TATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAC                               <  SRR4302540.8206302/94‑1 (MQ=60)
                                                                TATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAC                               >  SRR4302540.5204138/1‑94 (MQ=60)
                                                                TATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAC                               <  SRR4302540.5764956/94‑1 (MQ=60)
                                                                TATTGGCTCCATCAAAGCCGGT‑‑‑GA‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAC                               >  SRR4302540.448369/1‑94 (MQ=60)
                                                                 ATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACT                              >  SRR4302540.3169910/1‑94 (MQ=60)
                                                                 ATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACT                              >  SRR4302540.1285738/1‑94 (MQ=60)
                                                                 ATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACT                              >  SRR4302540.8127692/1‑94 (MQ=60)
                                                                 ATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACT                              >  SRR4302540.2413624/1‑94 (MQ=60)
                                                                  TTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTG                             >  SRR4302540.214002/1‑94 (MQ=60)
                                                                    GGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCG                           <  SRR4302540.231818/94‑1 (MQ=60)
                                                                    GGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCG                           <  SRR4302540.2224508/94‑1 (MQ=60)
                                                                    GGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCG                           <  SRR4302540.2220500/94‑1 (MQ=60)
                                                                    GGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCG                           >  SRR4302540.6864303/1‑94 (MQ=60)
                                                                     GCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGG                          <  SRR4302540.4021508/94‑1 (MQ=60)
                                                                       TCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTG                        <  SRR4302540.1782895/94‑1 (MQ=60)
                                                                        CCATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGG                       >  SRR4302540.8141580/1‑94 (MQ=60)
                                                                         CATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGC                      >  SRR4302540.9950753/1‑94 (MQ=60)
                                                                         CATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGC                      >  SRR4302540.7445391/1‑94 (MQ=60)
                                                                          ATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCG                     >  SRR4302540.8161853/1‑94 (MQ=60)
                                                                          ATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCG                     >  SRR4302540.9307749/1‑94 (MQ=60)
                                                                          ATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCG                     >  SRR4302540.5292699/1‑94 (MQ=60)
                                                                          ATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCG                     >  SRR4302540.4774275/1‑94 (MQ=60)
                                                                          ATCAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCG                     >  SRR4302540.3903235/1‑94 (MQ=60)
                                                                            CAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCA                   >  SRR4302540.7024004/1‑94 (MQ=60)
                                                                            CAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCA                   >  SRR4302540.6994745/1‑94 (MQ=60)
                                                                            CAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCA                   >  SRR4302540.6093807/1‑94 (MQ=60)
                                                                            CAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCA                   >  SRR4302540.5928440/1‑94 (MQ=60)
                                                                            CAAAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCA                   >  SRR4302540.9686867/1‑94 (MQ=60)
                                                                              AAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACA                 >  SRR4302540.5846443/1‑94 (MQ=60)
                                                                              AAGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACA                 >  SRR4302540.237185/1‑94 (MQ=60)
                                                                               AGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA                <  SRR4302540.1795221/94‑1 (MQ=60)
                                                                               AGCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA                >  SRR4302540.5355004/1‑94 (MQ=60)
                                                                                GCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAC               >  SRR4302540.1404428/1‑94 (MQ=60)
                                                                                GCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAC               <  SRR4302540.343496/94‑1 (MQ=60)
                                                                                GCCGGT‑‑‑GT‑‑‑C‑GGCGGGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAC               <  SRR4302540.9286883/94‑1 (MQ=60)
                                                                                                    GGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCAC  <  SRR4302540.2699288/94‑1 (MQ=60)
                                                                                                    GGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCAC  <  SRR4302540.3730531/94‑1 (MQ=60)
                                                                                                    GGAC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCAC  <  SRR4302540.7639643/94‑1 (MQ=60)
                                                                                                     |                                                                                             
TCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGT‑‑‑GT‑‑‑C‑G‑‑CGGAC‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCAC  >  NC_000913/3544268‑3544451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑