Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,573,700	+C	intergenic (+403/+997)	yhgN → / ← gntK	hypothetical protein/gluconokinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,573,700	1	.	C	99.6%	800.4 / NA	253	intergenic (+403/+997)	yhgN/gntK	hypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (119/132); minor base A (0/1); total (120/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑G‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

   aGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gctt                                                                                      <  1:68104/93‑4 (MQ=255)

   aGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gctt                                                                                      <  1:5001050/93‑4 (MQ=255)

    ggCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑gcttc                                                                                     >  1:5551582/1‑89 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGccc                                                                                 <  1:1830152/93‑1 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGATAAC‑GCTTCGccc                                                                                 >  1:101136/1‑93 (MQ=255)

          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  1:4435068/93‑1 (MQ=255)

          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCAAGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  2:1259695/93‑1 (MQ=255)

          ggTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGAAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAg                                                                               <  2:1750316/93‑1 (MQ=255)

           gTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGt                                                                              <  2:8031596/93‑1 (MQ=255)

            tGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGTCCATGCCGGTTAAC‑GCTTCGCCCAGTg                                                                             <  1:4358036/93‑1 (MQ=255)

            gggCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTg                                                                             <  1:1770614/92‑1 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           >  2:1045112/1‑93 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           >  2:241120/1‑93 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           <  2:4816974/93‑1 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGcc                                                                           <  2:4692246/93‑1 (MQ=255)

                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGa                                                                        <  2:381276/93‑1 (MQ=255)

                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGa                                                                        >  2:9180208/1‑93 (MQ=255)

                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAc                                                                       <  2:8902311/93‑1 (MQ=255)

                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAc                                                                       <  2:8372870/93‑1 (MQ=255)

                   aCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGAcc                                                                      <  1:594010/93‑1 (MQ=255)

                    cccTGAATGATGCGCACTGCGGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCt                                                                     <  1:2721937/93‑1 (MQ=255)

                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCTGACCTa                                                                    >  1:2084291/1‑93 (MQ=255)

                     ccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTa                                                                    <  2:4094249/93‑1 (MQ=255)

                      cTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTAc                                                                   <  2:5896899/93‑1 (MQ=255)

                       tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACg                                                                  >  2:2613506/1‑93 (MQ=255)

                         aaTGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGcc                                                                >  1:5278270/1‑93 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  2:6804738/1‑93 (MQ=255)

                           tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCag                                                              >  2:3485148/1‑93 (MQ=255)

                           tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCag                                                              <  1:8808482/93‑1 (MQ=255)

                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           <  2:3349627/93‑1 (MQ=255)

                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           >  1:3256073/1‑93 (MQ=255)

                              tgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGt                                                           >  1:7731115/1‑93 (MQ=255)

                               gcgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTc                                                          >  1:1661505/1‑93 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        <  2:6528140/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  2:4507729/93‑1 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  2:9752927/1‑93 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  2:5359649/93‑1 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  1:9787180/93‑1 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     <  1:8918527/93‑1 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  1:3002792/1‑93 (MQ=255)

                                    cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                                     >  1:4145808/1‑93 (MQ=255)

                                     tGCAGTTGCCAGCACGAAGCAGGAGATAGCAATCGGCAAGCCCATACCGGTTAAC‑GCTTGGCCCGATCCGGGACGTACGCCAGGGTCAGCCAg                                                    >  2:7888962/1‑93 (MQ=255)

                                      gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTAGCCCAGTGCCGGACCTACGACAGAGTCAACCAGc                                                   >  1:4217312/1‑93 (MQ=255)

                                        aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAc                                                 >  1:3769232/1‑93 (MQ=255)

                                          cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                                               <  1:3835978/93‑1 (MQ=255)

                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCCGAGTCAACCAGCACCTg                                              >  2:2182189/1‑93 (MQ=255)

                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                                              >  1:6090866/1‑93 (MQ=255)

                                           tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                                              <  2:1982212/93‑1 (MQ=255)

                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  1:8193798/93‑1 (MQ=255)

                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  2:762372/93‑1 (MQ=255)

                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGtt                                            <  1:3701327/93‑1 (MQ=255)

                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGGCCAGTGCCGGACCTACTCCAGAGACAACCAACACCTGtt                                            >  2:2400574/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:4366499/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:9540524/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:1899024/1‑93 (MQ=255)

                                                  aCGAATCATGTGATAGAAAACGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTAAGCCAGAGTCAACCAGCACCTGTTTGAac                                       <  1:8101939/93‑1 (MQ=255)

                                                  aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                                       >  2:1172724/1‑93 (MQ=255)

                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:6685614/93‑1 (MQ=255)

                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:6993929/93‑1 (MQ=255)

                                                   cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACACCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:7050706/93‑1 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTTCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  2:189015/93‑1 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:6847919/93‑1 (MQ=255)

                                                    caaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:7800418/92‑1 (MQ=255)

                                                     aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                                    <  1:5894030/93‑1 (MQ=255)

                                                       gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgcc                                  <  1:1258882/93‑1 (MQ=255)

                                                         aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc                                >  1:5369952/1‑93 (MQ=255)

                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               >  2:572029/1‑93 (MQ=255)

                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               >  1:596816/1‑93 (MQ=255)

                                                           gTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCCGTGCCGGACCTACGCCAGAGGCAACCAGCACCTGTTTGCACACGccgccc                              >  1:5177453/1‑93 (MQ=255)

                                                             gATAGCAATCGGCAGGCCCATGCCGGTTAACGG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTTGACACGccgcccgc                            >  2:2489192/1‑93 (MQ=255)

                                                             gATAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccgc                            >  2:7105051/1‑93 (MQ=255)

                                                              aTAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCa                           >  1:9487777/1‑93 (MQ=255)

                                                              aTAGCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCa                           <  1:4743177/93‑1 (MQ=255)

                                                                 gCAATCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCa                        >  1:8528780/1‑93 (MQ=255)

                                                                   aaTCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAt                      <  2:8408080/93‑1 (MQ=255)

                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    <  2:3969216/93‑1 (MQ=255)

                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    >  1:2557640/1‑93 (MQ=255)

                                                                     tCGGCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa                    >  2:1544817/1‑93 (MQ=255)

                                                                        gCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACca                 <  2:2274858/93‑1 (MQ=255)

                                                                        gCAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCAGTTTGAACACGCCGCCCGCACCAATCACca                 >  2:3026739/1‑93 (MQ=255)

                                                                         cAGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcag                >  2:4337704/1‑93 (MQ=255)

                                                                          aGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCTACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagc               >  2:4183312/1‑93 (MQ=255)

                                                                          aGGCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagc               <  2:9346090/93‑1 (MQ=255)

                                                                            gCCCATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagcag             >  2:3114410/1‑93 (MQ=255)

                                                                             cccATGCCGGTTATC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGa            <  2:689333/93‑1 (MQ=255)

                                                                              ccATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACTCGCACCTGTTTGAACACGACGCCCGCACCAATCACCAGCAGaa           >  2:1366954/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           <  1:5685722/93‑1 (MQ=255)

                                                                                 tGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        >  2:6665292/1‑93 (MQ=255)

                                                                                 tGCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        <  1:1058278/93‑1 (MQ=255)

                                                                                  gCCGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       >  1:6402918/1‑93 (MQ=255)

                                                                                    cGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcc     <  2:6216104/93‑1 (MQ=255)

                                                                                    cGGTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcc     <  2:8594899/93‑1 (MQ=255)

                                                                                     ggTTAAC‑NCTTCGCCCAGTGCNCGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:6321896/93‑1 (MQ=255)

                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    >  2:7410615/1‑93 (MQ=255)

                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    >  1:4105210/1‑93 (MQ=255)

                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  2:294271/93‑1 (MQ=255)

                                                                                     ggTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:9648436/93‑1 (MQ=255)

                                                                                      gTTAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   >  1:260103/1‑93 (MQ=255)

                                                                                       ttAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCccg  <  1:9572566/93‑1 (MQ=255)

                                                                                       ttAAC‑GCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCccg  >  1:9095207/1‑93 (MQ=255)

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAC‑G‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG                                                                                                <  SRR4302540.6229018/94‑1 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG                                                                                                >  SRR4302540.417165/1‑94 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAAG                                                                                                >  SRR4302540.901127/1‑94 (MQ=60)

 AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑C                                                                                              >  SRR4302540.834655/1‑94 (MQ=60)

 AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑C                                                                                              <  SRR4302540.9712522/94‑1 (MQ=60)

 AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACG‑C                                                                                              >  SRR4302540.6029903/1‑94 (MQ=60)

  ACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑CT                                                                                             >  SRR4302540.6958269/1‑94 (MQ=60)

  ACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACG‑CT                                                                                             >  SRR4302540.2855401/1‑94 (MQ=60)

   CAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGctt                                                                                             >  SRR4302540.5992966/1‑91 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGTACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            <  SRR4302540.2421858/94‑1 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            <  SRR4302540.5055205/94‑1 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            >  SRR4302540.1302375/1‑94 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            >  SRR4302540.5612737/1‑94 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            <  SRR4302540.68792/94‑1 (MQ=60)

     GGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCG                                                                                           >  SRR4302540.4747386/1‑94 (MQ=60)

     GGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCG                                                                                           >  SRR4302540.5640359/1‑94 (MQ=60)

      GCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGC                                                                                          >  SRR4302540.9431303/1‑94 (MQ=60)

        TACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGATAACGCTTCGCCC                                                                                        >  SRR4302540.102208/1‑94 (MQ=60)

         ACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCA                                                                                       <  SRR4302540.6762744/94‑1 (MQ=60)

         ACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCA                                                                                       <  SRR4302540.1850175/94‑1 (MQ=60)

         ACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCA                                                                                       <  SRR4302540.8209622/94‑1 (MQ=60)

          CGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAG                                                                                      <  SRR4302540.3061301/94‑1 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     <  SRR4302540.6020914/94‑1 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     <  SRR4302540.4483120/94‑1 (MQ=60)

             TGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGTCCATGCCGGTTAACGCTTCGCCCAGTGC                                                                                   <  SRR4302540.4405434/94‑1 (MQ=60)

             TGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGC                                                                                   <  SRR4302540.1790132/94‑1 (MQ=60)

             GGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGC                                                                                   <  SRR4302540.1789824/94‑1 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302540.7072759/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              >  SRR4302540.3840302/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              >  SRR4302540.5996360/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              >  SRR4302540.8122771/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              >  SRR4302540.1061159/1‑94 (MQ=60)

                   AACCCTGAATGATGCTCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACC                                                                             <  SRR4302540.2015805/94‑1 (MQ=60)

                    ACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCT                                                                            <  SRR4302540.600397/94‑1 (MQ=60)

                     CCCTGAATGATGCGCACTGCGGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTA                                                                           <  SRR4302540.2751283/94‑1 (MQ=60)

                     CCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCTGACCTA                                                                           >  SRR4302540.2106679/1‑94 (MQ=60)

                      CCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAC                                                                          <  SRR4302540.9620543/94‑1 (MQ=60)

                      CCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAC                                                                          <  SRR4302540.5427126/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         >  SRR4302540.5570679/1‑94 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGAGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCAAGTGACGGACCTACC                                                                         >  SRR4302540.2677307/1‑94 (MQ=60)

                        TGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGC                                                                        >  SRR4302540.4899068/1‑94 (MQ=60)

                         GAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC                                                                       >  SRR4302540.9138162/1‑94 (MQ=60)

                         GAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC                                                                       <  SRR4302540.8510284/94‑1 (MQ=60)

                         GAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC                                                                       >  SRR4302540.5335910/1‑94 (MQ=60)

                         GAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCC                                                                       >  SRR4302540.5328932/1‑94 (MQ=60)

                          aagtATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302540.8231504/90‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302540.9757587/94‑1 (MQ=60)

                            TGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGA                                                                    <  SRR4302540.444199/94‑1 (MQ=60)

                            TGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGA                                                                    <  SRR4302540.8910318/94‑1 (MQ=60)

                              ATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGT                                                                  >  SRR4302540.7821365/1‑94 (MQ=60)

                              ATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGT                                                                  >  SRR4302540.3291564/1‑94 (MQ=60)

                               TGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTC                                                                 >  SRR4302540.1679599/1‑94 (MQ=60)

                                  GCCCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGTTAACGCTCCGCCCAGTGCCGGACCTACGCCAGAGTCAAC                                                              <  SRR4302540.513289/94‑1 (MQ=60)

                                  GCACTGCAGCTGCCAGCACGAAGGAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAC                                                              <  SRR4302540.8813185/94‑1 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            >  SRR4302540.4190677/1‑94 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            >  SRR4302540.3035861/1‑94 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           <  SRR4302540.9021818/94‑1 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           <  SRR4302540.9901849/94‑1 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           <  SRR4302540.9901850/94‑1 (MQ=60)

                                      TGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGC                                                          <  SRR4302540.8979990/94‑1 (MQ=60)

                                      TGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTAGCCCAGTGCCGGACCTACGACAGAGTCAACCAGC                                                          >  SRR4302540.4263223/1‑94 (MQ=60)

                                       GCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCA                                                         <  SRR4302540.1792388/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        <  SRR4302540.8943063/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        <  SRR4302540.7113968/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        >  SRR4302540.3810355/1‑94 (MQ=60)

                                           CTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTG                                                     >  SRR4302540.608357/1‑94 (MQ=60)

                                           CTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTG                                                     >  SRR4302540.6158654/1‑94 (MQ=60)

                                           CTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTG                                                     <  SRR4302540.3877707/94‑1 (MQ=60)

                                            TGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGT                                                    <  SRR4302540.2844334/94‑1 (MQ=60)

                                             GCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTT                                                   <  SRR4302540.8289009/94‑1 (MQ=60)

                                             GCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTT                                                   >  SRR4302540.8097090/1‑94 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302540.3741734/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302540.4255610/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302540.8806358/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302540.6276267/94‑1 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  <  SRR4302540.6406595/94‑1 (MQ=60)

                                               CAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTG                                                 <  SRR4302540.434951/94‑1 (MQ=60)

                                                AGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGA                                                >  SRR4302540.5083618/1‑94 (MQ=60)

                                                 GCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAA                                               <  SRR4302540.4472122/94‑1 (MQ=60)

                                                 GCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAA                                               <  SRR4302540.9903598/94‑1 (MQ=60)

                                                 GCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAA                                               <  SRR4302540.2604940/94‑1 (MQ=60)

                                                   ACGAATCATGTGATAGAAAACGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAAGCCAGAGTCAACCAGCACCTGTTTGAACA                                             <  SRR4302540.8195975/94‑1 (MQ=60)

                                                   ACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACA                                             <  SRR4302540.1212584/94‑1 (MQ=60)

                                                   ACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACA                                             >  SRR4302540.9161620/1‑94 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.3662272/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.5870654/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.7071553/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.6759439/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.4007842/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACACCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302540.7129118/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302540.4064147/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302540.6923782/94‑1 (MQ=60)

                                                     CAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302540.7891591/94‑1 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGC                                          <  SRR4302540.5959127/94‑1 (MQ=60)

                                                       AGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCC                                         <  SRR4302540.353058/94‑1 (MQ=60)

                                                       AGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCC                                         <  SRR4302540.4993384/94‑1 (MQ=60)

                                                        GCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCG                                        <  SRR4302540.4774581/94‑1 (MQ=60)

                                                        GCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCG                                        <  SRR4302540.1271792/94‑1 (MQ=60)

                                                         CAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGC                                       >  SRR4302540.5428970/1‑94 (MQ=60)

                                                          AGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACGTACGCCAGAGTCAAACAGCACCTGTTTGAACACGCCGCC                                      >  SRR4302540.6125208/1‑94 (MQ=60)

                                                          AGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCC                                      >  SRR4302540.603226/1‑94 (MQ=60)

                                                           GGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGCCGGACCTACGCCAGAGGCAACCAGCACCTGTTTGCACACGCCGCCC                                     >  SRR4302540.5233804/1‑94 (MQ=60)

                                                           GGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCC                                     >  SRR4302540.4314269/1‑94 (MQ=60)

                                                           GGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCC                                     >  SRR4302540.1788472/1‑94 (MQ=60)

                                                             TGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGC                                   >  SRR4302540.2083177/1‑94 (MQ=60)

                                                              GATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCA                                  >  SRR4302540.9600261/1‑94 (MQ=60)

                                                              GATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCA                                  >  SRR4302540.2681515/1‑94 (MQ=60)

                                                               ATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAC                                 <  SRR4302540.4793796/94‑1 (MQ=60)

                                                                 AGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCA                               <  SRR4302540.9987713/94‑1 (MQ=60)

                                                                 AGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCA                               >  SRR4302540.8627878/1‑94 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            <  SRR4302540.6974003/94‑1 (MQ=60)

                                                                     ATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCA                           >  SRR4302540.2585205/1‑94 (MQ=60)

                                                                        GGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCA                        <  SRR4302540.5554924/94‑1 (MQ=60)

                                                                        GGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCA                        <  SRR4302540.1295999/94‑1 (MQ=60)

                                                                         GCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       <  SRR4302540.6761012/94‑1 (MQ=60)

                                                                           AGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCA                     >  SRR4302540.2244842/1‑94 (MQ=60)

                                                                            GGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAG                    <  SRR4302540.3796628/94‑1 (MQ=60)

                                                                             GCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGA                   >  SRR4302540.1725570/1‑94 (MQ=60)

                                                                             GCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGA                   >  SRR4302540.5846428/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACATGCCGCCCGCACCAATCACCAGCAGAA                  >  SRR4302540.7146754/1‑94 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302540.7847177/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302540.5748314/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302540.5748315/94‑1 (MQ=60)

                                                                                CATGCCGGTTAACGCTTCGCGCAGTGCCGGACCTACGCCATAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATG                <  SRR4302540.9892458/94‑1 (MQ=60)

                                                                                CATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATG                >  SRR4302540.6889594/1‑94 (MQ=60)

                                                                                 ATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGA               >  SRR4302540.9469104/1‑94 (MQ=60)

                                                                                 ATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGA               <  SRR4302540.1134508/94‑1 (MQ=60)

                                                                                  TGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAT              <  SRR4302540.1069054/94‑1 (MQ=60)

                                                                                  TGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAT              >  SRR4302540.5234652/1‑94 (MQ=60)

                                                                                  TGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAT              >  SRR4302540.6474189/1‑94 (MQ=60)

                                                                                  TGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGGCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAC              >  SRR4302540.6474188/1‑94 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATC             <  SRR4302540.5696318/94‑1 (MQ=60)

                                                                                    CCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCC            >  SRR4302540.6127097/1‑94 (MQ=60)

                                                                                    CCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCC            <  SRR4302540.7456590/94‑1 (MQ=60)

                                                                                     CGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCC           <  SRR4302540.6645388/94‑1 (MQ=60)

                                                                                     CGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCC           >  SRR4302540.3236431/1‑94 (MQ=60)

                                                                                     CGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCC           >  SRR4302540.4149500/1‑94 (MQ=60)

                                                                                      GGTTAACNCTTCGCCCAGTGCNCGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          <  SRR4302540.6392299/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACCCGCCGCCCGCACCAATCCCCAGCAGAATGATCCCC          <  SRR4302540.6666663/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          >  SRR4302540.262634/1‑94 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          <  SRR4302540.9762053/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          >  SRR4302540.4226948/1‑94 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         >  SRR4302540.3748288/1‑94 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         >  SRR4302540.9204480/1‑94 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302540.4657684/94‑1 (MQ=60)

                                                                                        TTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGC        <  SRR4302540.3877360/94‑1 (MQ=60)

                                                                                        TTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGC        >  SRR4302540.7645721/1‑94 (MQ=60)

                                                                                        TTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGC        <  SRR4302540.9685649/94‑1 (MQ=60)

                                                                                        TTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGC        <  SRR4302540.6892712/94‑1 (MQ=60)

                                                                                         TAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCC       >  SRR4302540.2614253/1‑94 (MQ=60)

                                                                                         TAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCC       <  SRR4302540.7608857/94‑1 (MQ=60)

                                                                                          AACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAGCAATCACCAGCAGAATGATCCCCGCCG      >  SRR4302540.10064151/1‑94 (MQ=60)

                                                                                          AACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCG      >  SRR4302540.8796319/1‑94 (MQ=60)

                                                                                          AACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCG      >  SRR4302540.8211612/1‑94 (MQ=60)

                                                                                           ACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGT     >  SRR4302540.7170456/1‑94 (MQ=60)

                                                                                           ACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGG     >  SRR4302540.6305617/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  SRR4302540.7739500/1‑94 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`