Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,573,700	+C	intergenic (+403/+997)	yhgN → / ← gntK	hypothetical protein/gluconokinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,573,700	1	.	C	99.5%	628.7 / ‑3.9	199	intergenic (+403/+997)	yhgN/gntK	hypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (105/93); total (106/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 212 total aligned reads displayed.

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

aaCAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTTACg                                                                                         >  1:4694168/1‑93 (MQ=255)

aaCAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                                         >  1:2707120/1‑93 (MQ=255)

  cAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgct                                                                                       <  2:5561775/93‑3 (MQ=255)

   aGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGGCCATGCCCGTTAACgctt                                                                                      >  2:1516848/1‑90 (MQ=255)

      cTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGc                                                                                   <  1:3097631/93‑1 (MQ=255)

       tACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGcc                                                                                  >  1:415206/1‑93 (MQ=255)

        aCGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGccc                                                                                 >  1:5308050/1‑93 (MQ=255)

         cGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCa                                                                                <  1:1040749/93‑1 (MQ=255)

            tGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCAGTTAACGCTTCGCCCAGTg                                                                             >  2:4505105/1‑93 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGcc                                                                           <  1:5735224/93‑1 (MQ=255)

              gCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGcc                                                                           >  1:3831781/1‑93 (MQ=255)

                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGa                                                                        <  1:2218162/93‑1 (MQ=255)

                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGa                                                                        >  1:1692289/1‑93 (MQ=255)

                 gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGa                                                                        >  1:5476240/1‑93 (MQ=255)

                  aaCCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAc                                                                       <  2:3124456/93‑1 (MQ=255)

                    cccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGCCGGACCt                                                                     >  2:2249924/1‑93 (MQ=255)

                      cTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                                   <  1:2516294/93‑1 (MQ=255)

                      cTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                                   <  2:3699261/93‑1 (MQ=255)

                      cTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGCCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                                   <  1:1929151/93‑1 (MQ=255)

                         aaTGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGcc                                                                <  1:3171811/93‑1 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  1:472533/1‑93 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  1:4240807/1‑93 (MQ=255)

                          atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCa                                                               >  2:6216009/1‑93 (MQ=255)

                             atgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCcgag                                                            >  2:3946785/1‑93 (MQ=255)

                             atgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCagag                                                            <  1:1441014/93‑1 (MQ=255)

                                cgcACTGCAGCTGCCAGCACGAAGCAGGTGATGGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCa                                                         <  2:1262550/93‑1 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        <  1:4581706/93‑1 (MQ=255)

                                 gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCaa                                                        >  1:3409823/1‑93 (MQ=255)

                                  cACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAc                                                       <  2:4418588/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  2:2345350/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  1:1737692/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      >  1:3218169/1‑93 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      <  2:4801702/93‑1 (MQ=255)

                                   aCTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                                      >  1:1220303/1‑93 (MQ=255)

                                     tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAg                                                    >  1:878589/1‑93 (MQ=255)

                                       cAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAgac                                                  >  2:2546848/1‑91 (MQ=255)

                                        aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAc                                                 >  1:3678445/1‑93 (MQ=255)

                                         gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                                                >  1:5182481/1‑93 (MQ=255)

                                         gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                                                >  1:5097216/1‑93 (MQ=255)

                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  2:1605515/93‑1 (MQ=255)

                                            gCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGt                                             <  1:2886543/93‑1 (MQ=255)

                                             ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGtt                                            <  2:3418842/93‑1 (MQ=255)

                                              cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGttt                                           <  2:3495203/93‑1 (MQ=255)

                                              cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGttt                                           >  1:5420951/1‑93 (MQ=255)

                                               aGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGTCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTg                                          >  2:5732088/1‑93 (MQ=255)

                                               aGCACGAAGCAGGTAATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTg                                          >  1:4873305/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  1:4846065/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:1727653/1‑93 (MQ=255)

                                                gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGa                                         >  2:2410377/1‑93 (MQ=255)

                                                 cACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                                        <  2:2032229/93‑1 (MQ=255)

                                                  aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                                       >  2:1961772/1‑93 (MQ=255)

                                                  aCGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                                       >  2:4749829/1‑93 (MQ=255)

                                                   cGAAGCAGGTGATAGCAATCGGCAGCCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAaca                                      <  1:4526216/93‑1 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     >  2:4134718/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     >  1:6292745/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     >  1:732256/1‑93 (MQ=255)

                                                    gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                                     <  1:1492553/93‑1 (MQ=255)

                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               >  1:4836779/1‑93 (MQ=255)

                                                          ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc                               <  2:3718501/93‑1 (MQ=255)

                                                             gATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccccccgc                            >  2:1292683/1‑93 (MQ=255)

                                                               tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc                          <  2:2291614/93‑1 (MQ=255)

                                                               tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc                          <  1:1270886/93‑1 (MQ=255)

                                                                aGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAcc                         <  1:6199297/93‑1 (MQ=255)

                                                                  cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCaa                       <  2:5946984/93‑1 (MQ=255)

                                                                   aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAt                      >  2:6106640/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     <  2:2779544/93‑1 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  1:933009/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  1:2361306/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  1:2818744/1‑93 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     <  1:5013320/93‑1 (MQ=255)

                                                                    aTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc                     >  2:4494765/1‑93 (MQ=255)

                                                                     tCGGCAGGCCCATGCCGGGTAACGCTTCGCCCAGTGCCGGAACTACGCCAGAGTCAACCAGCACCTGTTTGAACACGACCCCCGCACCAATCa                    >  2:3760972/1‑93 (MQ=255)

                                                                        gCAGTCCCATGCCGGTTAACGCTGCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACca                 <  1:5788762/93‑1 (MQ=255)

                                                                           ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagca              <  2:1998293/93‑1 (MQ=255)

                                                                           ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagca              >  1:5983425/1‑93 (MQ=255)

                                                                            gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACcagcag             >  2:3268740/1‑93 (MQ=255)

                                                                             cccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAACAGCAGa            >  1:2340196/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCTCCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           <  1:4549626/93‑1 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCTATCACCAGCAGaa           >  1:4003819/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           >  1:2646172/1‑93 (MQ=255)

                                                                              ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGaa           >  1:3554598/1‑93 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCCCCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          <  2:3680722/93‑1 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          <  1:2990439/93‑1 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          >  1:3090474/1‑93 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          <  1:3311196/93‑1 (MQ=255)

                                                                               cATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAt          >  1:3653190/1‑93 (MQ=255)

                                                                                aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATg         >  2:2649086/1‑93 (MQ=255)

                                                                                aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATg         >  2:5792336/1‑93 (MQ=255)

                                                                                 tGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGa        <  2:192407/93‑1 (MQ=255)

                                                                                  gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       <  2:5548617/93‑1 (MQ=255)

                                                                                  gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       <  2:5555390/93‑1 (MQ=255)

                                                                                  gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       <  2:3036317/93‑1 (MQ=255)

                                                                                  gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGAt       <  1:2247622/93‑1 (MQ=255)

                                                                                   ccGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATc      >  1:4058308/1‑93 (MQ=255)

                                                                                     ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    >  2:5133040/1‑93 (MQ=255)

                                                                                     ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:5675792/93‑1 (MQ=255)

                                                                                     ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATccc    <  1:1155274/93‑1 (MQ=255)

                                                                                      tttAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   <  2:5354811/92‑1 (MQ=255)

                                                                                      gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   <  1:3951353/93‑1 (MQ=255)

                                                                                      gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATcccc   >  2:4392541/1‑93 (MQ=255)

AACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG  >  NC_000913/3573608‑3573786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTTACG                                                                                                >  SRR4302533.4722613/1‑94 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG                                                                                                >  SRR4302533.2723728/1‑94 (MQ=60)

  ACAGGGTACGTTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑CT                                                                                             <  SRR4302533.5096552/94‑1 (MQ=60)

    AGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTC                                                                                            <  SRR4302533.3112641/94‑1 (MQ=60)

     GGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCG                                                                                           <  SRR4302533.5018021/94‑1 (MQ=60)

       CTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCC                                                                                         >  SRR4302533.417701/1‑94 (MQ=60)

       CTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCC                                                                                         <  SRR4302533.3116676/94‑1 (MQ=60)

       CTACGGGGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCC                                                                                         <  SRR4302533.3664723/94‑1 (MQ=60)

        TACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCC                                                                                        >  SRR4302533.4794998/1‑94 (MQ=60)

        TACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCC                                                                                        >  SRR4302533.5339862/1‑94 (MQ=60)

         ACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCA                                                                                       <  SRR4302533.520120/94‑1 (MQ=60)

          CGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAG                                                                                      <  SRR4302533.1046867/94‑1 (MQ=60)

           GGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGT                                                                                     >  SRR4302533.6242038/1‑94 (MQ=60)

             TGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGC                                                                                   >  SRR4302533.1731419/1‑94 (MQ=60)

              GGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCC                                                                                  >  SRR4302533.3855204/1‑94 (MQ=60)

               GCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCG                                                                                 <  SRR4302533.1544884/94‑1 (MQ=60)

               GCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCG                                                                                 <  SRR4302533.5769844/94‑1 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302533.1702542/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302533.5509250/1‑94 (MQ=60)

                 AGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAAAGCTTCGCCCAGTGCCGGA                                                                               >  SRR4302533.4018506/1‑94 (MQ=60)

                  GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGTCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAC                                                                              <  SRR4302533.2231856/94‑1 (MQ=60)

                    ACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCT                                                                            <  SRR4302533.4868900/94‑1 (MQ=60)

                      CCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAC                                                                          >  SRR4302533.829596/1‑94 (MQ=60)

                      CCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAC                                                                          <  SRR4302533.3257075/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302533.2531799/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302533.4264462/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         >  SRR4302533.711812/1‑94 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302533.573054/94‑1 (MQ=60)

                       CTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGCCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACG                                                                         <  SRR4302533.1940922/94‑1 (MQ=60)

                        TGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGC                                                                        >  SRR4302533.2644567/1‑94 (MQ=60)

                         GAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGCCCTACGCA                                                                       >  SRR4302533.4492400/1‑94 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302533.3191336/94‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      >  SRR4302533.4266416/1‑94 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302533.57835/94‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      <  SRR4302533.210914/94‑1 (MQ=60)

                          AATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCA                                                                      >  SRR4302533.475359/1‑94 (MQ=60)

                              ATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGT                                                                  <  SRR4302533.1449708/94‑1 (MQ=60)

                                GCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCA                                                                <  SRR4302533.1234334/94‑1 (MQ=60)

                                GCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCA                                                                <  SRR4302533.3570786/94‑1 (MQ=60)

                                 CGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAA                                                               >  SRR4302533.3430882/1‑94 (MQ=60)

                                  GCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAC                                                              <  SRR4302533.4609467/94‑1 (MQ=60)

                                   CACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACC                                                             >  SRR4302533.1227628/1‑94 (MQ=60)

                                   CACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACC                                                             >  SRR4302533.1798573/1‑94 (MQ=60)

                                   CACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACC                                                             >  SRR4302533.3238012/1‑94 (MQ=60)

                                    ACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCA                                                            <  SRR4302533.1748234/94‑1 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           >  SRR4302533.1482646/1‑94 (MQ=60)

                                     CTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAG                                                           >  SRR4302533.883745/1‑94 (MQ=60)

                                      TGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGC                                                          >  SRR4302533.5346263/1‑94 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        <  SRR4302533.5567651/94‑1 (MQ=60)

                                        CAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAC                                                        >  SRR4302533.3701055/1‑94 (MQ=60)

                                         AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACC                                                       <  SRR4302533.309632/94‑1 (MQ=60)

                                         AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACC                                                       >  SRR4302533.5128011/1‑94 (MQ=60)

                                         AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACC                                                       >  SRR4302533.4075092/1‑94 (MQ=60)

                                         AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACC                                                       >  SRR4302533.5213772/1‑94 (MQ=60)

                                          GCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCT                                                      <  SRR4302533.2854953/94‑1 (MQ=60)

                                          GCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCT                                                      >  SRR4302533.2314795/1‑94 (MQ=60)

                                             GCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTT                                                   <  SRR4302533.2904293/94‑1 (MQ=60)

                                             GCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTT                                                   >  SRR4302533.4893262/1‑94 (MQ=60)

                                              CCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTT                                                  >  SRR4302533.5453571/1‑94 (MQ=60)

                                               CAGCACGAAGCAGGTAATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTG                                                 >  SRR4302533.4902832/1‑94 (MQ=60)

                                                AGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGA                                                >  SRR4302533.4875434/1‑94 (MQ=60)

                                                  CACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAAC                                              >  SRR4302533.5904868/1‑94 (MQ=60)

                                                   ACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACA                                             <  SRR4302533.3137306/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            >  SRR4302533.736565/1‑94 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302533.1215795/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302533.2687625/94‑1 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            >  SRR4302533.6330261/1‑94 (MQ=60)

                                                    CGAAGCAGGTGATAGCAATCGGCAGCCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAC                                            <  SRR4302533.4553618/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302533.1501554/94‑1 (MQ=60)

                                                     GAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACG                                           <  SRR4302533.4669154/94‑1 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGC                                          <  SRR4302533.3198896/94‑1 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGC                                          >  SRR4302533.2858577/1‑94 (MQ=60)

                                                      AAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGC                                          >  SRR4302533.24934/1‑94 (MQ=60)

                                                       AGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCC                                         >  SRR4302533.5291184/1‑94 (MQ=60)

                                                        GCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCG                                        <  SRR4302533.3863409/94‑1 (MQ=60)

                                                         CAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGC                                       >  SRR4302533.4591509/1‑94 (MQ=60)

                                                          AGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCC                                      >  SRR4302533.4866095/1‑94 (MQ=60)

                                                          AGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCC                                      >  SRR4302533.1098646/1‑94 (MQ=60)

                                                            GTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCG                                    >  SRR4302533.3367994/1‑94 (MQ=60)

                                                               ATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAC                                 >  SRR4302533.2996192/1‑94 (MQ=60)

                                                                TAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACC                                <  SRR4302533.1278488/94‑1 (MQ=60)

                                                                 AGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCA                               <  SRR4302533.6236483/94‑1 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            >  SRR4302533.2836090/1‑94 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            >  SRR4302533.938486/1‑94 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            <  SRR4302533.2220271/94‑1 (MQ=60)

                                                                    AATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATC                            >  SRR4302533.2375894/1‑94 (MQ=60)

                                                                     ATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCA                           <  SRR4302533.5043694/94‑1 (MQ=60)

                                                                       CGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACC                         >  SRR4302533.2261462/1‑94 (MQ=60)

                                                                         GCAGTCCCATGCCGGTTAACGCTGCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       <  SRR4302533.5823738/94‑1 (MQ=60)

                                                                         GCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTATGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAG                       <  SRR4302533.6232706/94‑1 (MQ=60)

                                                                           AGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCA                     >  SRR4302533.5497529/1‑94 (MQ=60)

                                                                           AGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCA                     >  SRR4302533.4989216/1‑94 (MQ=60)

                                                                           AGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCA                     >  SRR4302533.6019661/1‑94 (MQ=60)

                                                                             GCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAACAGCAGA                   >  SRR4302533.2354687/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCTATCACCAGCAGAA                  >  SRR4302533.4028196/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAA                  >  SRR4302533.2662432/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAA                  >  SRR4302533.3576501/1‑94 (MQ=60)

                                                                              CCCATGCCGGTTAACGCTTCGCCCAGCGCCGGACCTACGCCCGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCCATCACCAGCAGAA                  >  SRR4302533.3576500/1‑94 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCTCCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302533.4577213/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 >  SRR4302533.3109474/1‑94 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 <  SRR4302533.908793/94‑1 (MQ=60)

                                                                               CCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAAT                 >  SRR4302533.3675653/1‑94 (MQ=60)

                                                                                CATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATG                <  SRR4302533.3331642/94‑1 (MQ=60)

                                                                                CATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATG                <  SRR4302533.3008730/94‑1 (MQ=60)

                                                                                CATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATG                <  SRR4302533.3708497/94‑1 (MQ=60)

                                                                                 ATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGA               >  SRR4302533.4752868/1‑94 (MQ=60)

                                                                                 ATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGA               <  SRR4302533.4569329/94‑1 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATC             >  SRR4302533.4083014/1‑94 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATC             <  SRR4302533.2261511/94‑1 (MQ=60)

                                                                                   GCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATC             <  SRR4302533.1371761/94‑1 (MQ=60)

                                                                                     CGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCC           <  SRR4302533.3697162/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          <  SRR4302533.5710072/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          <  SRR4302533.1162203/94‑1 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          >  SRR4302533.4083285/1‑94 (MQ=60)

                                                                                      GGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCC          <  SRR4302533.3445889/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302533.3975441/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302533.2769087/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         >  SRR4302533.4312432/1‑94 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCG         <  SRR4302533.2358049/94‑1 (MQ=60)

                                                                                       GTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCC         >  SRR4302533.5785155/1‑94 (MQ=60)

                                                                                         TAACGCTTCGCCCAGTGCCGGAGCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCC       <  SRR4302533.5109771/94‑1 (MQ=60)

                                                                                           ACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGG     >  SRR4302533.1375795/1‑94 (MQ=60)

                                                                                           ACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGG     >  SRR4302533.492067/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  SRR4302533.5098761/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  SRR4302533.2244789/1‑94 (MQ=60)

                                                                                             C‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  SRR4302533.5494767/1‑94 (MQ=60)

AAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTG  >  NC_000913/3573607‑3573793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`