Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,502 A→G T189T (ACT→ACC)  b3454 ← ATP‑binding component of leucine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,5020AG99.2% 1167.6 / ‑1.9 362T189T (ACT→ACC) b3454ATP‑binding component of leucine transport
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (145/214);  total (147/215)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 372 total aligned reads displayed.

ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591
                                                                                          |                                                                                         
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  2:3070236/93‑1 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  1:6389201/93‑1 (MQ=255)
 tCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTc                                                                                        >  1:700928/1‑93 (MQ=255)
  cGGAAGGCACTACAGGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  2:8422287/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:4285124/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:6718267/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:648641/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:3593295/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  2:8081298/93‑1 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGATGAGAAAGATGGTCATc                                                                                     >  1:1835096/1‑93 (MQ=255)
     aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcc                                                                                    >  2:3402293/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  >  1:6030525/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  2:248914/93‑1 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  1:1914485/93‑1 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               <  1:3355300/93‑1 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTc                                                                             >  1:2726865/1‑93 (MQ=255)
             aCATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCttg                                                                            >  2:1017613/1‑91 (MQ=255)
             aCATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcg                                                                            <  1:5778213/93‑1 (MQ=255)
               aTGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgcg                                                                          <  1:9922926/93‑1 (MQ=255)
                tGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         >  2:1514514/1‑93 (MQ=255)
                tGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         <  1:852903/93‑1 (MQ=255)
                  gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAg                                                                       >  1:7851406/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:7608442/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:8158026/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:2209861/1‑93 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  2:9791036/93‑1 (MQ=255)
                          ccAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGa                                                               <  1:94508/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:6510795/93‑1 (MQ=255)
                            cgCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             <  2:3511962/92‑1 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             >  2:1379692/1‑93 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  1:538576/93‑1 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCATTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  2:5526040/93‑1 (MQ=255)
                              cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                           <  2:9932858/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          >  2:9651702/1‑93 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  2:8456385/93‑1 (MQ=255)
                                cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtgt                                                         <  2:281659/93‑1 (MQ=255)
                                  tAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCg                                                       >  1:2574745/1‑93 (MQ=255)
                                    gCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaa                                                     >  2:608124/1‑93 (MQ=255)
                                    gCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaa                                                     >  1:3152811/1‑93 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGGGTCGAAAAttt                                                >  1:2831102/1‑93 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCACTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAttt                                                >  2:8774659/1‑93 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               <  2:1468059/93‑1 (MQ=255)
                                             cGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTg                                            <  1:5000367/93‑1 (MQ=255)
                                             cGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTAATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTg                                            <  2:2926637/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  1:7360110/1‑93 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  2:4999149/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  1:10046447/1‑93 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         <  2:5241002/93‑1 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGAAGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         >  2:2709847/1‑93 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        <  1:1209169/93‑1 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        >  1:1650800/1‑93 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        <  1:5698817/93‑1 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        >  1:3309229/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       >  1:8193573/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       <  2:1621510/93‑1 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     <  1:9218897/93‑1 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     >  2:6828251/1‑93 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  2:7688213/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAt                                   <  1:2058571/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAt                                   <  1:834106/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCACGCGCAGCTGCTCGATGGTGTCGAACATTTGCTGGATGATAAt                                   >  2:4797690/1‑93 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:9110078/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:6805455/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTGCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  2:3839447/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 >  2:6732351/1‑93 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  2:2553528/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  2:4028135/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                >  2:2112792/1‑93 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:5091877/93‑1 (MQ=255)
                                                          gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgc                               <  2:7151484/93‑1 (MQ=255)
                                                           ccTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcg                              <  2:9542028/93‑1 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTTGATGATAATCGgcgc                             >  1:1935497/1‑93 (MQ=255)
                                                             tGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCa                            >  1:4743839/1‑93 (MQ=255)
                                                              ggTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCaa                           <  2:3900168/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          <  2:6434691/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          <  1:5866462/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          >  1:5043696/1‑93 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:8119652/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  2:4258409/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAgg                   >  2:1851832/1‑92 (MQ=255)
                                                                      ttCTGCTCGAAGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   >  2:9085683/1‑93 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  2:7434904/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGCGCGAt                >  1:2263955/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:4613034/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  1:4392376/93‑1 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               >  1:1977588/1‑93 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               <  1:3814626/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  1:891503/93‑1 (MQ=255)
                                                                              gacgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtc           <  1:6255795/93‑1 (MQ=255)
                                                                              gacgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtc           >  1:5104548/1‑93 (MQ=255)
                                                                               acgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtca          <  1:4110478/93‑1 (MQ=255)
                                                                               acgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtca          <  2:8176014/93‑1 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGCGCGAAGGCtcatc        >  2:7957206/1‑93 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatc        <  1:9822360/93‑1 (MQ=255)
                                                                                  agaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatca       <  2:3269092/93‑1 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      <  1:393664/93‑1 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      >  2:5593069/1‑93 (MQ=255)
                                                                                    aaaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAg     <  2:2353697/93‑1 (MQ=255)
                                                                                     aaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc    >  2:734922/1‑93 (MQ=255)
                                                                                      aGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCa   <  1:3014198/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  >  NC_000913/3590410‑3590595
                                                                                            |                                                                                             
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                              <  SRR4302540.4820821/94‑1 (MQ=60)
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                              <  SRR4302540.7897353/94‑1 (MQ=60)
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                              >  SRR4302540.395509/1‑94 (MQ=60)
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                              <  SRR4302540.344848/94‑1 (MQ=60)
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATACGCCCGCTTTAGCGGCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                              >  SRR4302540.395510/1‑94 (MQ=60)
 TATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGT                                                                                             >  SRR4302540.8808760/1‑94 (MQ=60)
 TATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGT                                                                                             >  SRR4302540.2013100/1‑94 (MQ=60)
  ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            >  SRR4302540.708453/1‑94 (MQ=60)
  ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            >  SRR4302540.5221173/1‑94 (MQ=60)
  ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            >  SRR4302540.2883766/1‑94 (MQ=60)
  ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            <  SRR4302540.6460323/94‑1 (MQ=60)
   TCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCA                                                                                           <  SRR4302540.2186554/94‑1 (MQ=60)
    CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.275655/94‑1 (MQ=60)
    CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.4331735/94‑1 (MQ=60)
    CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.6200401/94‑1 (MQ=60)
    CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.6792387/94‑1 (MQ=60)
    CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.655545/94‑1 (MQ=60)
    CGGAAAGCACTACATCGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302540.5404865/94‑1 (MQ=60)
     GGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGATGAGAAAGATGGTCATC                                                                                         >  SRR4302540.1855184/1‑94 (MQ=60)
     GGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATC                                                                                         <  SRR4302540.3120916/94‑1 (MQ=60)
     GGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATC                                                                                         >  SRR4302540.1185221/1‑94 (MQ=60)
      GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                        >  SRR4302540.198971/1‑94 (MQ=60)
      GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                        <  SRR4302540.3861596/94‑1 (MQ=60)
       AAAGCACTACATGGCCGTTTTCCAGCTGGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       <  SRR4302540.9377451/94‑1 (MQ=60)
       AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       <  SRR4302540.4725229/94‑1 (MQ=60)
       AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       <  SRR4302540.8146409/94‑1 (MQ=60)
        AAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCC                                                                                      >  SRR4302540.6097447/1‑94 (MQ=60)
        AAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCC                                                                                      <  SRR4302540.5146781/94‑1 (MQ=60)
         AGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCT                                                                                     <  SRR4302540.1935384/94‑1 (MQ=60)
         AGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCT                                                                                     >  SRR4302540.3940887/1‑94 (MQ=60)
            ACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCT                                                                                  <  SRR4302540.3392070/94‑1 (MQ=60)
            ACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCT                                                                                  >  SRR4302540.826688/1‑94 (MQ=60)
             CTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTC                                                                                 >  SRR4302540.2756277/1‑94 (MQ=60)
             CTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTC                                                                                 <  SRR4302540.7083509/94‑1 (MQ=60)
               ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                               >  SRR4302540.1686134/1‑94 (MQ=60)
               ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                               >  SRR4302540.5875932/1‑94 (MQ=60)
               ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                               <  SRR4302540.5842043/94‑1 (MQ=60)
               ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                               <  SRR4302540.2167574/94‑1 (MQ=60)
               ACATGGCCGTGTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCACCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                               <  SRR4302540.7988480/94‑1 (MQ=60)
                 ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                             >  SRR4302540.3377164/1‑94 (MQ=60)
                 ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                             >  SRR4302540.7057481/1‑94 (MQ=60)
                 ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                             <  SRR4302540.10038790/94‑1 (MQ=60)
                  TGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCA                                                                            <  SRR4302540.861956/94‑1 (MQ=60)
                  TGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCA                                                                            <  SRR4302540.861955/94‑1 (MQ=60)
                   GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           >  SRR4302540.7943149/1‑94 (MQ=60)
                   GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           >  SRR4302540.2854331/1‑94 (MQ=60)
                   GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           <  SRR4302540.4938154/94‑1 (MQ=60)
                   GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           <  SRR4302540.2521982/94‑1 (MQ=60)
                   GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           >  SRR4302540.8214840/1‑94 (MQ=60)
                     CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                         <  SRR4302540.2273971/94‑1 (MQ=60)
                      CGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTG                                                                        <  SRR4302540.70889/94‑1 (MQ=60)
                       GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       >  SRR4302540.2233729/1‑94 (MQ=60)
                       GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       >  SRR4302540.8252740/1‑94 (MQ=60)
                       GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       >  SRR4302540.7693940/1‑94 (MQ=60)
                       GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       >  SRR4302540.6192823/1‑94 (MQ=60)
                        TTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCT                                                                      <  SRR4302540.5071889/94‑1 (MQ=60)
                         TTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTC                                                                     <  SRR4302540.3993217/94‑1 (MQ=60)
                         TTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTC                                                                     <  SRR4302540.6855176/94‑1 (MQ=60)
                          TTCCAGCACGTAGGCGCGGTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCG                                                                    <  SRR4302540.4868386/94‑1 (MQ=60)
                           TCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGA                                                                   >  SRR4302540.827452/1‑94 (MQ=60)
                           TCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGA                                                                   <  SRR4302540.8593363/94‑1 (MQ=60)
                            CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  <  SRR4302540.95510/94‑1 (MQ=60)
                            CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  <  SRR4302540.7659893/94‑1 (MQ=60)
                            CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  <  SRR4302540.3311554/94‑1 (MQ=60)
                              AGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGG                                                                <  SRR4302540.10067912/94‑1 (MQ=60)
                               GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               <  SRR4302540.544401/94‑1 (MQ=60)
                               GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               <  SRR4302540.9297849/94‑1 (MQ=60)
                               GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               >  SRR4302540.8221210/1‑94 (MQ=60)
                                CACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTG                                                              <  SRR4302540.5309698/94‑1 (MQ=60)
                                CACGAAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTG                                                              <  SRR4302540.9247532/94‑1 (MQ=60)
                                 ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                             >  SRR4302540.3381833/1‑94 (MQ=60)
                                 ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                             >  SRR4302540.256171/1‑94 (MQ=60)
                                 ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                             >  SRR4302540.8095/1‑94 (MQ=60)
                                 ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                             >  SRR4302540.751461/1‑94 (MQ=60)
                                 ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGC                                                             >  SRR4302540.3098494/1‑94 (MQ=60)
                                  CGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTC                                                            >  SRR4302540.4340484/1‑94 (MQ=60)
                                  CGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTC                                                            <  SRR4302540.9148014/94‑1 (MQ=60)
                                   GTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCG                                                           >  SRR4302540.2602497/1‑94 (MQ=60)
                                   GGAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCG                                                           <  SRR4302540.1916884/94‑1 (MQ=60)
                                     AGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAA                                                         >  SRR4302540.3187134/1‑94 (MQ=60)
                                       CCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAA                                                       <  SRR4302540.5895419/94‑1 (MQ=60)
                                        CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                      >  SRR4302540.7578853/1‑94 (MQ=60)
                                         GCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATT                                                     >  SRR4302540.7867535/1‑94 (MQ=60)
                                          CGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGGGTCGAAAATTT                                                    >  SRR4302540.2861722/1‑94 (MQ=60)
                                           GATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTG                                                   >  SRR4302540.3991684/1‑94 (MQ=60)
                                             TCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCT                                                 <  SRR4302540.3995225/94‑1 (MQ=60)
                                             TCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCT                                                 <  SRR4302540.7783595/94‑1 (MQ=60)
                                              CCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTG                                                <  SRR4302540.5661206/94‑1 (MQ=60)
                                               CGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGG                                               <  SRR4302540.5054511/94‑1 (MQ=60)
                                               CGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGG                                               >  SRR4302540.9322842/1‑94 (MQ=60)
                                                GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                              >  SRR4302540.7442182/1‑94 (MQ=60)
                                                GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                              <  SRR4302540.6872132/94‑1 (MQ=60)
                                                GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                              >  SRR4302540.10163236/1‑94 (MQ=60)
                                                 GCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                             <  SRR4302540.3348778/94‑1 (MQ=60)
                                                 CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                             <  SRR4302540.299959/94‑1 (MQ=60)
                                                  CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGGGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            >  SRR4302540.5594972/1‑94 (MQ=60)
                                                  CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            <  SRR4302540.2609359/94‑1 (MQ=60)
                                                  CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            >  SRR4302540.1668800/1‑94 (MQ=60)
                                                  CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            >  SRR4302540.3345484/1‑94 (MQ=60)
                                                   AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                           <  SRR4302540.5761565/94‑1 (MQ=60)
                                                   AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                           <  SRR4302540.1221574/94‑1 (MQ=60)
                                                   AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                           >  SRR4302540.8288779/1‑94 (MQ=60)
                                                    GCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGAT                                          <  SRR4302540.1664243/94‑1 (MQ=60)
                                                    GCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGAT                                          >  SRR4302540.3158903/1‑94 (MQ=60)
                                                    GCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGAAGGTCATCCCCTTCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGAT                                          <  SRR4302540.4187631/94‑1 (MQ=60)
                                                     CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                         <  SRR4302540.5098194/94‑1 (MQ=60)
                                                     CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                         <  SRR4302540.3524335/94‑1 (MQ=60)
                                                      TTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAA                                        <  SRR4302540.9329475/94‑1 (MQ=60)
                                                       TTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAT                                       <  SRR4302540.6028386/94‑1 (MQ=60)
                                                        TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302540.842964/94‑1 (MQ=60)
                                                        TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302540.842965/94‑1 (MQ=60)
                                                        TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302540.1687668/94‑1 (MQ=60)
                                                        TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302540.2080728/94‑1 (MQ=60)
                                                        TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302540.4246338/94‑1 (MQ=60)
                                                         AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302540.6880759/94‑1 (MQ=60)
                                                         AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302540.8695711/94‑1 (MQ=60)
                                                         AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302540.9111756/94‑1 (MQ=60)
                                                         AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302540.9219561/94‑1 (MQ=60)
                                                         AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302540.7069858/94‑1 (MQ=60)
                                                          GGGCCTGGTTGGCGTTCTGCTCGACGAGAAAGGTGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                    <  SRR4302540.8397719/94‑1 (MQ=60)
                                                          GCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                    <  SRR4302540.3505198/94‑1 (MQ=60)
                                                           CGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGC                                   <  SRR4302540.3645431/94‑1 (MQ=60)
                                                            GCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTAGATGGTGTCGAAAATTTGCTGGATGATAATCGGCG                                  <  SRR4302540.1771178/94‑1 (MQ=60)
                                                             CCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTTGATGATAATCGGCGC                                 >  SRR4302540.1956583/1‑94 (MQ=60)
                                                             CCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGC                                 >  SRR4302540.8929329/1‑94 (MQ=60)
                                                              CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                                >  SRR4302540.1903754/1‑94 (MQ=60)
                                                              CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                                >  SRR4302540.4794462/1‑94 (MQ=60)
                                                              CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                                >  SRR4302540.3935577/1‑94 (MQ=60)
                                                              CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                                >  SRR4302540.6734667/1‑94 (MQ=60)
                                                               TGGTTGGCGTGCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAA                               <  SRR4302540.2236277/94‑1 (MQ=60)
                                                                GGTTGGCGTTCTGCTCGGCGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATCATCGGCGCAAG                              >  SRR4302540.9942124/1‑94 (MQ=60)
                                                                GGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAG                              >  SRR4302540.5098253/1‑94 (MQ=60)
                                                                GGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAG                              <  SRR4302540.6162708/94‑1 (MQ=60)
                                                                 GTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGA                             <  SRR4302540.5931318/94‑1 (MQ=60)
                                                                   ggccaTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.5948578/89‑1 (MQ=60)
                                                                   TGGCGTTCTGCTCGACTAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATCGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.1103685/94‑1 (MQ=60)
                                                                   TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.2649850/94‑1 (MQ=60)
                                                                   TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.8213879/94‑1 (MQ=60)
                                                                   TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.4387905/94‑1 (MQ=60)
                                                                   TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302540.4890639/94‑1 (MQ=60)
                                                                    GGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATTGCGTCGAAAATTTGCTGGCTGATAATCGGCGCAAGACCG                          >  SRR4302540.1948125/1‑94 (MQ=60)
                                                                    GGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCG                          >  SRR4302540.1948126/1‑94 (MQ=60)
                                                                    GGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCA                          <  SRR4302540.5758739/94‑1 (MQ=60)
                                                                     GCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGA                         <  SRR4302540.6453260/94‑1 (MQ=60)
                                                                     GCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCGGGATGATAATCGGCGCAAGACCGA                         <  SRR4302540.4885354/94‑1 (MQ=60)
                                                                     GCCTTCTGCTCGACGGTAAAGATTGTCATCCCCTGCTCGCGCAGCTGCTCAATGCTTTCGAAAATTTGCTGGATGATAGTCGGCGCAAGACCGA                         <  SRR4302540.6452950/94‑1 (MQ=60)
                                                                      CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                        >  SRR4302540.9664999/1‑94 (MQ=60)
                                                                       GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCTGCGCAACACCGAGC                       >  SRR4302540.8225073/1‑94 (MQ=60)
                                                                       GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                       <  SRR4302540.1937025/94‑1 (MQ=60)
                                                                       GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                       >  SRR4302540.9511896/1‑94 (MQ=60)
                                                                        TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCGCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                      <  SRR4302540.3044542/94‑1 (MQ=60)
                                                                         TCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGA                     <  SRR4302540.1572043/94‑1 (MQ=60)
                                                                         TCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGA                     >  SRR4302540.10086142/1‑94 (MQ=60)
                                                                          CTGCTCGGCGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302540.6703281/94‑1 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGCGCGAT                    >  SRR4302540.2288251/1‑94 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    >  SRR4302540.5851689/1‑94 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302540.1984997/94‑1 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302540.4211297/94‑1 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302540.6746723/94‑1 (MQ=60)
                                                                          CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302540.6393506/94‑1 (MQ=60)
                                                                           TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   <  SRR4302540.4440039/94‑1 (MQ=60)
                                                                           TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   >  SRR4302540.5972235/1‑94 (MQ=60)
                                                                           TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   >  SRR4302540.1999043/1‑94 (MQ=60)
                                                                            GCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGG                  <  SRR4302540.5093721/94‑1 (MQ=60)
                                                                            GCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGG                  <  SRR4302540.3856162/94‑1 (MQ=60)
                                                                             CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                 <  SRR4302540.900977/94‑1 (MQ=60)
                                                                             CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                 >  SRR4302540.2441980/1‑94 (MQ=60)
                                                                               CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               >  SRR4302540.4500821/1‑94 (MQ=60)
                                                                               CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               <  SRR4302540.6483322/94‑1 (MQ=60)
                                                                               CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               >  SRR4302540.5159808/1‑94 (MQ=60)
                                                                                GACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCA              <  SRR4302540.6325404/94‑1 (MQ=60)
                                                                                GACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCA              <  SRR4302540.5799152/94‑1 (MQ=60)
                                                                                 ACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCAT             <  SRR4302540.4154848/94‑1 (MQ=60)
                                                                                 ACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCAT             >  SRR4302540.1275200/1‑94 (MQ=60)
                                                                                  CGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC            >  SRR4302540.2029186/1‑94 (MQ=60)
                                                                                  CGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC            <  SRR4302540.8299332/94‑1 (MQ=60)
                                                                                   GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCA           <  SRR4302540.9937277/94‑1 (MQ=60)
                                                                                   GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCA           <  SRR4302540.2036920/94‑1 (MQ=60)
                                                                                   GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCA           <  SRR4302540.9661078/94‑1 (MQ=60)
                                                                                   GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTAATCA           >  SRR4302540.6054923/1‑94 (MQ=60)
                                                                                   GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAATTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCA           <  SRR4302540.9661079/94‑1 (MQ=60)
                                                                                     GAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAG         <  SRR4302540.397739/94‑1 (MQ=60)
                                                                                       AAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCA       >  SRR4302540.375226/1‑94 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      >  SRR4302540.9334540/1‑94 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.6461933/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.3047342/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.3198271/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.3625047/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.5119750/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      >  SRR4302540.5786255/1‑94 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.7427374/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.7198064/94‑1 (MQ=60)
                                                                                        AGATGGTCATCCCCTGCTCCCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGCCCGAGCGATGGCTCATCAAGCAG      <  SRR4302540.2263277/94‑1 (MQ=60)
                                                                                          ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    <  SRR4302540.9165205/94‑1 (MQ=60)
                                                                                          ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    <  SRR4302540.9015260/94‑1 (MQ=60)
                                                                                          ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    <  SRR4302540.7918316/94‑1 (MQ=60)
                                                                                          ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    >  SRR4302540.6440257/1‑94 (MQ=60)
                                                                                          ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    >  SRR4302540.10003972/1‑94 (MQ=60)
                                                                                           TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG   >  SRR4302540.6591954/1‑94 (MQ=60)
                                                                                            GGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGA  >  SRR4302540.359629/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  >  NC_000913/3590410‑3590595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑