Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,502 A→G T189T (ACT→ACC)  b3454 ← ATP‑binding component of leucine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,5020AG100.0% 891.6 / NA 270T189T (ACT→ACC) b3454ATP‑binding component of leucine transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (116/154);  total (116/154)

Only 100 of 274 total aligned reads displayed.

ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591
                                                                                          |                                                                                         
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  2:1800699/93‑1 (MQ=255)
 tCGGAAAGCACTATATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTc                                                                                        <  1:4897520/93‑1 (MQ=255)
 tCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTc                                                                                        <  2:2202687/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:3172785/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       >  2:4399821/1‑93 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATc                                                                                     <  1:886905/93‑1 (MQ=255)
     aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcc                                                                                    <  1:3852697/93‑1 (MQ=255)
     aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcc                                                                                    >  1:2204464/1‑93 (MQ=255)
      aaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATccc                                                                                   >  1:2076506/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  2:5122604/93‑1 (MQ=255)
        gCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCt                                                                                 >  1:2984598/1‑93 (MQ=255)
         cACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTg                                                                                >  1:2327151/1‑93 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               >  1:4022667/1‑93 (MQ=255)
           cTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCt                                                                              <  1:2899543/93‑1 (MQ=255)
           cTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCt                                                                              <  2:4135027/93‑1 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCTCCCAGCTTTAGAGCCTGGTTGGCGTTCTGCTCGACGCGAAAGATGGTCATCCCCCGCTc                                                                             >  2:5702514/1‑93 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCAGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTc                                                                             >  2:4555931/1‑93 (MQ=255)
              cATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgc                                                                           >  1:5299907/1‑93 (MQ=255)
              cATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgc                                                                           <  2:5232679/93‑1 (MQ=255)
               aTGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgcg                                                                          <  1:1629550/93‑1 (MQ=255)
                tgcccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         <  2:4734745/90‑1 (MQ=255)
                tGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         <  1:18636/93‑1 (MQ=255)
                  gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAg                                                                       <  2:1692006/93‑1 (MQ=255)
                  gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAg                                                                       >  2:359418/1‑93 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  2:2515105/93‑1 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  1:4679290/93‑1 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  2:5809767/93‑1 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  2:1877775/93‑1 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCACCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  2:3830023/93‑1 (MQ=255)
                     gTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctg                                                                    >  1:4464755/1‑93 (MQ=255)
                     gTTTTCCAGCACGAAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGCAAGATGGTCATCCCCTGCTCGCGCAgctg                                                                    >  2:6191886/1‑93 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  2:2671140/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:975650/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  1:3614617/93‑1 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             <  1:5070074/93‑1 (MQ=255)
                             gaaCGTCGCCGCGATCCGCCACCTTTATCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  2:1112060/91‑1 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  2:532931/93‑1 (MQ=255)
                              cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                           <  2:854812/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  1:1272857/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          >  2:3006540/1‑93 (MQ=255)
                                 gTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTc                                                        >  2:1046127/1‑93 (MQ=255)
                                  tAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCg                                                       <  1:3759601/93‑1 (MQ=255)
                                   aGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGa                                                      >  1:5318908/1‑93 (MQ=255)
                                       gcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAt                                                  >  2:3299779/1‑93 (MQ=255)
                                       gcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAt                                                  >  1:1178107/1‑93 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               <  1:3909901/93‑1 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               >  2:4421285/1‑93 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           <  1:4577204/93‑1 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           >  2:2836408/1‑93 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           >  2:2721845/1‑93 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTTGAAAATTTGCTggg                                          >  2:1592046/1‑92 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  2:508614/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  1:2647457/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  2:429564/1‑93 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  2:5510388/1‑93 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        <  1:1935021/93‑1 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        >  2:574475/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       >  1:6089834/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       >  1:5927338/1‑93 (MQ=255)
                                                   cTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatgat                                      >  1:5535281/1‑93 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     <  2:2896724/93‑1 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  1:2788408/93‑1 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  2:4506077/93‑1 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  2:1196227/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  >  2:3054433/1‑93 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:5135403/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:3942069/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  1:4958169/93‑1 (MQ=255)
                                                          gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgc                               <  1:6233006/93‑1 (MQ=255)
                                                           ccTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcg                              <  2:4689944/93‑1 (MQ=255)
                                                            cTGGTTTGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGATGTCGAAAATTTGCTGGATGATAATCGgcgc                             <  2:5005123/93‑1 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCTCCTGCTCGTGCAGCTGCTCGATGGAGTCGAAAATTTGCTGGATGATAATCGgcgc                             >  2:3513374/1‑93 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcgc                             <  2:1309158/93‑1 (MQ=255)
                                                              ggTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCaa                           >  1:409741/1‑93 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          >  2:5870604/1‑93 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:1318060/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        >  1:1505579/1‑93 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:3456980/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  2:4800082/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:3352995/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        >  1:4520197/1‑93 (MQ=255)
                                                                  ggCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAcc                       >  2:6225957/1‑93 (MQ=255)
                                                                  ggCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAcc                       >  1:4154828/1‑93 (MQ=255)
                                                                    cGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGa                     <  1:2549485/93‑1 (MQ=255)
                                                                    cGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGa                     <  1:1409729/93‑1 (MQ=255)
                                                                     gTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAg                    <  2:6040337/93‑1 (MQ=255)
                                                                     gTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAg                    <  2:3951777/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   <  1:5453860/93‑1 (MQ=255)
                                                                       tCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCg                  <  2:3879494/93‑1 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 >  1:1690827/1‑93 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  1:57616/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:5364862/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  1:4804703/1‑93 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  1:5994536/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  2:6011911/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  1:2401074/93‑1 (MQ=255)
                                                                               acgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtca          <  2:3335497/93‑1 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatc        >  1:5694848/1‑93 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatc        <  2:2291356/93‑1 (MQ=255)
                                                                                  agaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatca       >  2:1441391/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG  >  NC_000913/3590409‑3590594
                                                                                             |                                                                                            
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCTCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                              <  SRR4302533.584236/94‑1 (MQ=60)
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                              >  SRR4302533.3051716/1‑94 (MQ=60)
 GTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGG                                                                                             <  SRR4302533.4218052/94‑1 (MQ=60)
    TCGGAAAGCACTATATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCA                                                                                          <  SRR4302533.4927195/94‑1 (MQ=60)
     CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                         <  SRR4302533.3192316/94‑1 (MQ=60)
     CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                         >  SRR4302533.6048332/1‑94 (MQ=60)
      GGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATC                                                                                        <  SRR4302533.1401623/94‑1 (MQ=60)
       GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                       <  SRR4302533.892111/94‑1 (MQ=60)
       GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                       >  SRR4302533.2218051/1‑94 (MQ=60)
       GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                       >  SRR4302533.1397943/1‑94 (MQ=60)
       GAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCC                                                                                       >  SRR4302533.5110704/1‑94 (MQ=60)
        AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                      >  SRR4302533.2089287/1‑94 (MQ=60)
        AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                      <  SRR4302533.3876226/94‑1 (MQ=60)
         AAGCACTACATGGCCCTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCC                                                                                     <  SRR4302533.2068354/94‑1 (MQ=60)
          AGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCT                                                                                    <  SRR4302533.90155/94‑1 (MQ=60)
          AGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCT                                                                                    >  SRR4302533.3002855/1‑94 (MQ=60)
           GCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTG                                                                                   >  SRR4302533.1671042/1‑94 (MQ=60)
           GCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTG                                                                                   >  SRR4302533.2341557/1‑94 (MQ=60)
           GCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTG                                                                                   <  SRR4302533.228110/94‑1 (MQ=60)
            CACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGC                                                                                  >  SRR4302533.4047160/1‑94 (MQ=60)
             ACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCT                                                                                 <  SRR4302533.3532014/94‑1 (MQ=60)
              CTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTC                                                                                <  SRR4302533.2917361/94‑1 (MQ=60)
                ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                              >  SRR4302533.5331656/1‑94 (MQ=60)
                ACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGC                                                                              <  SRR4302533.5288683/94‑1 (MQ=60)
                 CATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCTTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCG                                                                             <  SRR4302533.2287752/94‑1 (MQ=60)
                 CATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCG                                                                             >  SRR4302533.3842210/1‑94 (MQ=60)
                 CAGGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCG                                                                             <  SRR4302533.2771917/94‑1 (MQ=60)
                  ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                            <  SRR4302533.1639418/94‑1 (MQ=60)
                  ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                            >  SRR4302533.4841610/1‑94 (MQ=60)
                  ATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGC                                                                            >  SRR4302533.3807010/1‑94 (MQ=60)
                   TGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCA                                                                           >  SRR4302533.1653045/1‑94 (MQ=60)
                   TGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCA                                                                           <  SRR4302533.18761/94‑1 (MQ=60)
                      CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                        <  SRR4302533.4707650/94‑1 (MQ=60)
                       CGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTG                                                                       >  SRR4302533.4669354/1‑94 (MQ=60)
                       CGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTG                                                                       >  SRR4302533.4491759/1‑94 (MQ=60)
                          TTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTC                                                                    <  SRR4302533.2849745/94‑1 (MQ=60)
                           TTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCG                                                                   <  SRR4302533.2653240/94‑1 (MQ=60)
                             CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                 <  SRR4302533.3675601/94‑1 (MQ=60)
                             CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                 >  SRR4302533.3114827/1‑94 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                <  SRR4302533.4127897/94‑1 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                <  SRR4302533.1430947/94‑1 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                <  SRR4302533.3636859/94‑1 (MQ=60)
                               AGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGG                                                               <  SRR4302533.5100688/94‑1 (MQ=60)
                               AGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGG                                                               <  SRR4302533.5374106/94‑1 (MQ=60)
                                GCACGTAGCCGCGATCCGCCAGCTGTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                              <  SRR4302533.5825696/94‑1 (MQ=60)
                                 CACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTG                                                             <  SRR4302533.5759492/94‑1 (MQ=60)
                                 CACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGCCATCCCCTGCTCGCGCAGCTGCTCGGTTGTG                                                             >  SRR4302533.4438086/1‑94 (MQ=60)
                                  ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                            <  SRR4302533.1280475/94‑1 (MQ=60)
                                  ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                            <  SRR4302533.2051064/94‑1 (MQ=60)
                                  ACGTACCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                            <  SRR4302533.1627181/94‑1 (MQ=60)
                                   CGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTC                                                           <  SRR4302533.6014591/94‑1 (MQ=60)
                                    GTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCG                                                          >  SRR4302533.2132895/1‑94 (MQ=60)
                                    GTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCG                                                          >  SRR4302533.1764339/1‑94 (MQ=60)
                                     TAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGA                                                         >  SRR4302533.5350785/1‑94 (MQ=60)
                                     TAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGA                                                         <  SRR4302533.3782646/94‑1 (MQ=60)
                                        CCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAA                                                      <  SRR4302533.6295676/94‑1 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                     <  SRR4302533.3920354/94‑1 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                     >  SRR4302533.1185182/1‑94 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                     <  SRR4302533.276457/94‑1 (MQ=60)
                                            GATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGGAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTG                                                  >  SRR4302533.4464056/1‑94 (MQ=60)
                                            GATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTG                                                  >  SRR4302533.3297258/1‑94 (MQ=60)
                                            GATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTG                                                  >  SRR4302533.1853070/1‑94 (MQ=60)
                                             CTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGC                                                 <  SRR4302533.1293816/94‑1 (MQ=60)
                                             ATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGC                                                 <  SRR4302533.3933779/94‑1 (MQ=60)
                                              TCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCT                                                <  SRR4302533.6291439/94‑1 (MQ=60)
                                               CCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTG                                               <  SRR4302533.6014627/94‑1 (MQ=60)
                                                 GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                             <  SRR4302533.4604944/94‑1 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                            >  SRR4302533.4746494/1‑94 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                            >  SRR4302533.4546965/1‑94 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                            <  SRR4302533.4079176/94‑1 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                            >  SRR4302533.2446429/1‑94 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                            <  SRR4302533.2663722/94‑1 (MQ=60)
                                                    AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGCTGGTGTCGAAAATTTGCTGGATGA                                          >  SRR4302533.4472993/1‑94 (MQ=60)
                                                    AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                          >  SRR4302533.5963171/1‑94 (MQ=60)
                                                    AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                          <  SRR4302533.1946825/94‑1 (MQ=60)
                                                    AGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGA                                          >  SRR4302533.6126644/1‑94 (MQ=60)
                                                    AGATTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGGCATCCCCTGGTCGCGCAGCATCTCGATGGTGTggcaaatttgttgtcgta                                          >  SRR4302533.6126643/1‑76 (MQ=60)
                                                     GCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGAT                                         <  SRR4302533.3962472/94‑1 (MQ=60)
                                                     GCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGAT                                         >  SRR4302533.5568702/1‑94 (MQ=60)
                                                      CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                        >  SRR4302533.191948/1‑94 (MQ=60)
                                                      CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                        >  SRR4302533.6031523/1‑94 (MQ=60)
                                                       CTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAA                                       <  SRR4302533.5194629/94‑1 (MQ=60)
                                                        TTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAT                                      <  SRR4302533.2805543/94‑1 (MQ=60)
                                                          AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                    <  SRR4302533.3966121/94‑1 (MQ=60)
                                                          AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                    <  SRR4302533.5166433/94‑1 (MQ=60)
                                                           GCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                   <  SRR4302533.1634812/94‑1 (MQ=60)
                                                           GCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                   <  SRR4302533.4988205/94‑1 (MQ=60)
                                                           GCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                   <  SRR4302533.3419134/94‑1 (MQ=60)
                                                            CGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGC                                  <  SRR4302533.3966118/94‑1 (MQ=60)
                                                             GCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCG                                 <  SRR4302533.4749526/94‑1 (MQ=60)
                                                             GCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCG                                 <  SRR4302533.6270329/94‑1 (MQ=60)
                                                              CCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGC                                <  SRR4302533.4533757/94‑1 (MQ=60)
                                                               CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                               >  SRR4302533.5649698/1‑94 (MQ=60)
                                                               CTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCA                               >  SRR4302533.5016413/1‑94 (MQ=60)
                                                                TGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAA                              >  SRR4302533.412198/1‑94 (MQ=60)
                                                                 GGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTTGATGATAATCGGCGCAAG                             <  SRR4302533.4134242/94‑1 (MQ=60)
                                                                  GTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGA                            <  SRR4302533.3120547/94‑1 (MQ=60)
                                                                   TTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAC                           >  SRR4302533.1514660/1‑94 (MQ=60)
                                                                   TTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAC                           >  SRR4302533.4547569/1‑94 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          <  SRR4302533.2424185/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          <  SRR4302533.3478323/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          <  SRR4302533.3373710/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          <  SRR4302533.1325967/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          <  SRR4302533.3755733/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                          >  SRR4302533.4180005/1‑94 (MQ=60)
                                                                     GGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCG                         <  SRR4302533.3974604/94‑1 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                       <  SRR4302533.1418252/94‑1 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                       <  SRR4302533.246566/94‑1 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                       <  SRR4302533.2565179/94‑1 (MQ=60)
                                                                        GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                      >  SRR4302533.534514/1‑94 (MQ=60)
                                                                        GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                      >  SRR4302533.237011/1‑94 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     >  SRR4302533.3547633/1‑94 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     <  SRR4302533.2933800/94‑1 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     <  SRR4302533.2933801/94‑1 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     <  SRR4302533.4700193/94‑1 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     <  SRR4302533.5486711/94‑1 (MQ=60)
                                                                         TTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCG                     <  SRR4302533.5493718/94‑1 (MQ=60)
                                                                          TCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGA                    >  SRR4302533.1701070/1‑94 (MQ=60)
                                                                          TCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGA                    >  SRR4302533.3971612/1‑94 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                   <  SRR4302533.57998/94‑1 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                   >  SRR4302533.4833799/1‑94 (MQ=60)
                                                                            TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                  >  SRR4302533.4931344/1‑94 (MQ=60)
                                                                             GCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGG                 >  SRR4302533.263328/1‑94 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                <  SRR4302533.2415903/94‑1 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                <  SRR4302533.6030843/94‑1 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                >  SRR4302533.1263014/1‑94 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                >  SRR4302533.486033/1‑94 (MQ=60)
                                                                                CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC              >  SRR4302533.1319660/1‑94 (MQ=60)
                                                                                CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGCGTCGAAAATTTGCTGGATGCTAATCGGCGCAAGACCGAGCGATGGCTC              >  SRR4302533.5159373/1‑94 (MQ=60)
                                                                                 GACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCA             <  SRR4302533.5548770/94‑1 (MQ=60)
                                                                                  ACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCACGACCGAGCGATGGCTCAT            >  SRR4302533.2207369/1‑94 (MQ=60)
                                                                                  ACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCAT            >  SRR4302533.5057436/1‑94 (MQ=60)
                                                                                   CGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC           >  SRR4302533.6193470/1‑94 (MQ=60)
                                                                                   CGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC           <  SRR4302533.5379275/94‑1 (MQ=60)
                                                                                   CGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC           >  SRR4302533.5729240/1‑94 (MQ=60)
                                                                                    GAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCCAGACCGAGCGATGGCTCATCA          >  SRR4302533.5927054/1‑94 (MQ=60)
                                                                                      GAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAG        <  SRR4302533.5619070/94‑1 (MQ=60)
                                                                                      GAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAG        <  SRR4302533.3285614/94‑1 (MQ=60)
                                                                                          GATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGTAGT    <  SRR4302533.456379/94‑1 (MQ=60)
                                                                                          GATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGT    >  SRR4302533.3153893/1‑94 (MQ=60)
                                                                                           ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA   <  SRR4302533.1759040/94‑1 (MQ=60)
                                                                                           ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA   <  SRR4302533.633925/94‑1 (MQ=60)
                                                                                            TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG  >  SRR4302533.3227165/1‑94 (MQ=60)
                                                                                            TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG  <  SRR4302533.5148077/94‑1 (MQ=60)
                                                                                             |                                                                                            
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG  >  NC_000913/3590409‑3590594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑