Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,628,759 G→A R488C (CGT→TGT)  yhiJ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,628,7590GA99.8% 2945.7 / ‑5.0 790R488C (CGT→TGT) yhiJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (426/362);  total (427/363)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 820 total aligned reads displayed.

AGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA  >  NC_000913/3628667‑3628848
                                                                                            |                                                                                         
  aCTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACagg                                                                                         <  1:6983539/93‑1 (MQ=255)
  aCTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACagg                                                                                         <  2:3840502/93‑1 (MQ=255)
  aCTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACagg                                                                                         <  1:3644505/93‑1 (MQ=255)
   cTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGc                                                                                        <  2:1473153/93‑1 (MQ=255)
    ttCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCg                                                                                       >  1:1837683/1‑93 (MQ=255)
    ttCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCg                                                                                       >  1:5096661/1‑93 (MQ=255)
    ttCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCg                                                                                       <  2:6764839/93‑1 (MQ=255)
     tCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGt                                                                                      >  2:1407727/1‑93 (MQ=255)
       cAGGGAGTGGTTATCTCGGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTa                                                                                    <  2:5036048/93‑1 (MQ=255)
       cAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTa                                                                                    <  1:7617224/93‑1 (MQ=255)
       cAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTa                                                                                    >  2:8675841/1‑93 (MQ=255)
        aGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTaa                                                                                   <  2:9627662/93‑1 (MQ=255)
             gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              >  1:205845/1‑93 (MQ=255)
              tGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTa                                                                             >  2:9045940/1‑93 (MQ=255)
              tGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTa                                                                             >  1:6103319/1‑93 (MQ=255)
              tGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTa                                                                             >  1:2517330/1‑93 (MQ=255)
               ggTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGGTAAGACCTaa                                                                            >  2:6977608/1‑93 (MQ=255)
                 ttATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTaaaa                                                                          <  1:997578/93‑1 (MQ=255)
                  tATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAAc                                                                         <  2:9501450/93‑1 (MQ=255)
                    tctcAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTg                                                                       >  2:3653803/1‑93 (MQ=255)
                      tcAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGATCTAAAACTGTc                                                                     >  1:7266138/1‑93 (MQ=255)
                       cAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTcc                                                                    <  2:805281/93‑1 (MQ=255)
                         ggCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAGCATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTcccc                                                                  >  2:33695/1‑93 (MQ=255)
                          gCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCg                                                                 >  1:676067/1‑93 (MQ=255)
                           gccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATCCTTCCAGCAGGTTTCCTGTCGCAGGCGTTAATACCTATAACTGGCCCCGt                                                                <  2:8779702/92‑1 (MQ=255)
                           cccTGCTCCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGt                                                                <  1:10026105/93‑1 (MQ=255)
                            ccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGtt                                                               <  1:7151064/93‑1 (MQ=255)
                              cgCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTc                                                             <  2:2772671/92‑1 (MQ=255)
                                  ccAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCt                                                         >  1:9556763/1‑93 (MQ=255)
                                  ccAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCt                                                         <  1:8885203/93‑1 (MQ=255)
                                   cAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTa                                                        >  2:3961762/1‑93 (MQ=255)
                                   cAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTa                                                        >  2:6096339/1‑93 (MQ=255)
                                   cAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTa                                                        <  2:8543216/93‑1 (MQ=255)
                                    aGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTAt                                                       >  2:9314884/1‑93 (MQ=255)
                                    aGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTAt                                                       <  1:4877625/93‑1 (MQ=255)
                                     gggTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATg                                                      >  1:2737541/1‑93 (MQ=255)
                                      ggTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGc                                                     <  2:4834117/93‑1 (MQ=255)
                                      ggTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGc                                                     >  1:3530027/1‑93 (MQ=255)
                                       gTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCg                                                    <  1:6337775/93‑1 (MQ=255)
                                       gTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCg                                                    >  2:2041042/1‑93 (MQ=255)
                                         tAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGtt                                                  >  1:6227045/1‑93 (MQ=255)
                                         tAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGtt                                                  <  1:4341191/93‑1 (MQ=255)
                                          aaTTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtc                                                 >  2:2456320/1‑93 (MQ=255)
                                            tttGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtctc                                               >  1:8097715/1‑93 (MQ=255)
                                            tttGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtctc                                               <  2:7795979/93‑1 (MQ=255)
                                            tttGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtctc                                               <  1:2359080/93‑1 (MQ=255)
                                              tagCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCtt                                             <  1:5521978/91‑1 (MQ=255)
                                              tGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCtt                                             >  1:1193745/1‑93 (MQ=255)
                                              tGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCtt                                             <  1:921031/93‑1 (MQ=255)
                                              tGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACGGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCtt                                             <  1:6888450/93‑1 (MQ=255)
                                                gCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTcc                                           <  1:8510228/93‑1 (MQ=255)
                                                    ttATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacca                                       >  1:2232206/1‑93 (MQ=255)
                                                      atgcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCAtt                                     <  1:9368991/93‑1 (MQ=255)
                                                       tgcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCAttt                                    >  2:8275930/1‑93 (MQ=255)
                                                        tcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTCTCCCCGTTGCCCCTATGCGTTCTCTTCCTCCATTTc                                   <  1:5649834/92‑1 (MQ=255)
                                                        gcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTc                                   <  1:7747846/93‑1 (MQ=255)
                                                        gcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTc                                   <  2:185134/93‑1 (MQ=255)
                                                          atgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCg                                 <  2:5899675/93‑1 (MQ=255)
                                                           tgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                >  1:6506626/1‑93 (MQ=255)
                                                           tgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                >  1:583006/1‑93 (MQ=255)
                                                           tgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                >  1:5305816/1‑93 (MQ=255)
                                                           tgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                >  1:2925703/1‑93 (MQ=255)
                                                             cTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTa                              <  1:9034344/93‑1 (MQ=255)
                                                             cTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTa                              <  2:3495338/93‑1 (MQ=255)
                                                              tGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAt                             >  1:1491498/1‑93 (MQ=255)
                                                               gAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAtg                            >  1:9819518/1‑93 (MQ=255)
                                                                 aCATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAtgtg                          <  1:3900711/93‑1 (MQ=255)
                                                                   aTTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGca                        <  2:1120436/93‑1 (MQ=255)
                                                                    ttcttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaa                       >  2:9816096/1‑93 (MQ=255)
                                                                    ttcttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaa                       <  2:2611165/93‑1 (MQ=255)
                                                                    ttcttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaa                       >  1:4115510/1‑93 (MQ=255)
                                                                    ttcttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaa                       >  2:568132/1‑93 (MQ=255)
                                                                       ttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaacaa                    <  2:3088156/93‑1 (MQ=255)
                                                                         ccAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACt                  >  1:379443/1‑93 (MQ=255)
                                                                          cAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACtt                 <  2:4717385/93‑1 (MQ=255)
                                                                          cAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACtt                 <  2:6346062/93‑1 (MQ=255)
                                                                           aGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTg                >  2:4153949/1‑93 (MQ=255)
                                                                           aGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTg                >  2:7233547/1‑93 (MQ=255)
                                                                           aGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTg                >  1:7948803/1‑93 (MQ=255)
                                                                            gCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGa               <  2:1691170/93‑1 (MQ=255)
                                                                             cATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAt              >  1:7430708/1‑93 (MQ=255)
                                                                              aTGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAtt             >  2:2248370/1‑93 (MQ=255)
                                                                              aTGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAtt             >  1:4546514/1‑93 (MQ=255)
                                                                              aTGTTTACTGTCACAGGCGTTAAGACCCAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAtt             >  2:3267593/1‑93 (MQ=255)
                                                                               tGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAttt            <  1:8355379/93‑1 (MQ=255)
                                                                               tGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAttt            <  2:661222/93‑1 (MQ=255)
                                                                                gtgtACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           <  2:5425355/90‑1 (MQ=255)
                                                                                gTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           >  2:5277684/1‑93 (MQ=255)
                                                                                 tttACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTtat          >  2:2153085/1‑93 (MQ=255)
                                                                                     cTGTCACAGGCGTTAAGTCCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCt      <  1:1678721/93‑1 (MQ=255)
                                                                                     cTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCt      >  2:7836079/1‑93 (MQ=255)
                                                                                     cTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCt      >  1:6005115/1‑93 (MQ=255)
                                                                                      tGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTa     >  2:5196410/1‑93 (MQ=255)
                                                                                      tGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTa     >  1:3331673/1‑93 (MQ=255)
                                                                                       gTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTaa    <  2:7591115/93‑1 (MQ=255)
                                                                                       gTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTaa    <  1:8778121/93‑1 (MQ=255)
                                                                                        tCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAAc   >  2:7391354/1‑93 (MQ=255)
                                                                                        tCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAAc   <  1:1390614/93‑1 (MQ=255)
                                                                                         cacaGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACa  >  1:6592526/1‑93 (MQ=255)
                                                                                         cacaGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACa  <  2:4551348/93‑1 (MQ=255)
                                                                                            |                                                                                         
AGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA  >  NC_000913/3628667‑3628848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 451 total aligned reads displayed.
AAGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTG  >  NC_000913/3628666‑3628852
                                                                                             |                                                                                             
AAGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACA                                                                                               <  SRR4302540.4612181/94‑1 (MQ=60)
AAGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACA                                                                                               <  SRR4302540.1925256/94‑1 (MQ=60)
AAGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACA                                                                                               >  SRR4302540.7613951/1‑94 (MQ=60)
 AGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAG                                                                                              >  SRR4302540.9612187/1‑94 (MQ=60)
 AGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAG                                                                                              <  SRR4302540.1808523/94‑1 (MQ=60)
 AGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAG                                                                                              <  SRR4302540.2879475/94‑1 (MQ=60)
  GACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGG                                                                                             <  SRR4302540.3324924/94‑1 (MQ=60)
  AACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGG                                                                                             <  SRR4302540.8746660/94‑1 (MQ=60)
   ACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGC                                                                                            <  SRR4302540.3684428/94‑1 (MQ=60)
   ACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGC                                                                                            <  SRR4302540.7061095/94‑1 (MQ=60)
   ACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGC                                                                                            >  SRR4302540.8071929/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.6636995/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.6215812/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.1857808/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.9702282/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.5151813/1‑94 (MQ=60)
    CTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCG                                                                                           >  SRR4302540.4907080/1‑94 (MQ=60)
     TTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGT                                                                                          >  SRR4302540.1874056/1‑94 (MQ=60)
       CCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTA                                                                                        >  SRR4302540.564559/1‑94 (MQ=60)
         AGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAG                                                                                      <  SRR4302540.7624834/94‑1 (MQ=60)
         AGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAG                                                                                      <  SRR4302540.5416273/94‑1 (MQ=60)
          GGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGTGTTAAGA                                                                                     <  SRR4302540.6911490/94‑1 (MQ=60)
           GGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGAC                                                                                    >  SRR4302540.9213714/1‑94 (MQ=60)
           GGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGAC                                                                                    >  SRR4302540.2693480/1‑94 (MQ=60)
            GAGTTGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACC                                                                                   <  SRR4302540.7402286/94‑1 (MQ=60)
            GAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACC                                                                                   <  SRR4302540.6040454/94‑1 (MQ=60)
             AGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCT                                                                                  >  SRR4302540.8293412/1‑94 (MQ=60)
             AGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCT                                                                                  >  SRR4302540.207864/1‑94 (MQ=60)
             AGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCT                                                                                  <  SRR4302540.2393488/94‑1 (MQ=60)
              GTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTA                                                                                 >  SRR4302540.10058866/1‑94 (MQ=60)
              GTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTA                                                                                 >  SRR4302540.2544519/1‑94 (MQ=60)
              GTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTA                                                                                 >  SRR4302540.6009193/1‑94 (MQ=60)
              GTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTA                                                                                 >  SRR4302540.6749389/1‑94 (MQ=60)
              GTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGGTTACTGTCACAGGCGTTAAGACCTA                                                                                 >  SRR4302540.3836083/1‑94 (MQ=60)
               TGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAA                                                                                <  SRR4302540.3924700/94‑1 (MQ=60)
               TGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAA                                                                                <  SRR4302540.1655917/94‑1 (MQ=60)
               TGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAA                                                                                <  SRR4302540.8595174/94‑1 (MQ=60)
                GGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAA                                                                               <  SRR4302540.3777411/94‑1 (MQ=60)
                GGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAA                                                                               >  SRR4302540.1142508/1‑94 (MQ=60)
                 GTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAA                                                                              <  SRR4302540.2181862/94‑1 (MQ=60)
                  TTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAAC                                                                             >  SRR4302540.5079850/1‑94 (MQ=60)
                   TATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACT                                                                            <  SRR4302540.6324591/94‑1 (MQ=60)
                    ATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTG                                                                           <  SRR4302540.9968648/94‑1 (MQ=60)
                    ATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTG                                                                           <  SRR4302540.5523661/94‑1 (MQ=60)
                     TCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGT                                                                          <  SRR4302540.7279872/94‑1 (MQ=60)
                     TCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGT                                                                          <  SRR4302540.5109134/94‑1 (MQ=60)
                      CTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTC                                                                         >  SRR4302540.302368/1‑94 (MQ=60)
                      CTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTC                                                                         >  SRR4302540.7943825/1‑94 (MQ=60)
                       TCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCC                                                                        >  SRR4302540.7315114/1‑94 (MQ=60)
                        CAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCC                                                                       <  SRR4302540.4324740/94‑1 (MQ=60)
                        CAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCC                                                                       >  SRR4302540.7330571/1‑94 (MQ=60)
                        CAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCC                                                                       >  SRR4302540.3793988/1‑94 (MQ=60)
                         AGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCC                                                                      <  SRR4302540.5672457/94‑1 (MQ=60)
                         AGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCC                                                                      <  SRR4302540.5801568/94‑1 (MQ=60)
                         AGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCC                                                                      <  SRR4302540.5926290/94‑1 (MQ=60)
                         AGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCC                                                                      <  SRR4302540.6346129/94‑1 (MQ=60)
                         AGGCCCTGCACCAGGGATAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTAACAGGCGTTAAGACCTAAAACTGTCCCC                                                                      >  SRR4302540.8367391/1‑94 (MQ=60)
                           GCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGT                                                                    <  SRR4302540.936716/94‑1 (MQ=60)
                           GCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGT                                                                    >  SRR4302540.9760282/1‑94 (MQ=60)
                           GCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGT                                                                    <  SRR4302540.747841/94‑1 (MQ=60)
                            CCCTGCTCCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTT                                                                   <  SRR4302540.10142767/94‑1 (MQ=60)
                            CCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTT                                                                   <  SRR4302540.10142769/94‑1 (MQ=60)
                            CCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTT                                                                   >  SRR4302540.7562733/1‑94 (MQ=60)
                             CCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTT                                                                  <  SRR4302540.7230754/94‑1 (MQ=60)
                             CCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTT                                                                  <  SRR4302540.5833224/94‑1 (MQ=60)
                             CCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTAACAGGCGTTAAGACCTAAAACTGTCCCCGTTT                                                                  >  SRR4302540.8950402/1‑94 (MQ=60)
                             CCTGCACCAGGGTTAATTTGGCAATTATGCAAGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTT                                                                  >  SRR4302540.1897485/1‑94 (MQ=60)
                              CTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTC                                                                 >  SRR4302540.351882/1‑94 (MQ=60)
                              CTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTC                                                                 >  SRR4302540.5256271/1‑94 (MQ=60)
                              CTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTC                                                                 <  SRR4302540.6627093/94‑1 (MQ=60)
                               ttgcaCAGGGTGAATGTGGCAATTATGCATGCTGAACAGTCTTCCAGCATGTTGACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCG                                                                <  SRR4302540.7626389/89‑1 (MQ=60)
                               TGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCG                                                                >  SRR4302540.3811977/1‑94 (MQ=60)
                                GCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGC                                                               <  SRR4302540.9937945/94‑1 (MQ=60)
                                 CACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCC                                                              >  SRR4302540.6139975/1‑94 (MQ=60)
                                 CACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCC                                                              >  SRR4302540.8134942/1‑94 (MQ=60)
                                 CACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCC                                                              <  SRR4302540.7845602/94‑1 (MQ=60)
                                  ACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCTCCT                                                             >  SRR4302540.7717000/1‑94 (MQ=60)
                                  ACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCT                                                             >  SRR4302540.6542675/1‑94 (MQ=60)
                                  ACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCT                                                             >  SRR4302540.9669773/1‑94 (MQ=60)
                                  ACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCT                                                             <  SRR4302540.1302191/94‑1 (MQ=60)
                                  ACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCT                                                             >  SRR4302540.9448876/1‑94 (MQ=60)
                                   CCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTA                                                            >  SRR4302540.9001214/1‑94 (MQ=60)
                                   CCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTA                                                            >  SRR4302540.2263601/1‑94 (MQ=60)
                                   CCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTA                                                            >  SRR4302540.8847273/1‑94 (MQ=60)
                                   CCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTA                                                            >  SRR4302540.2413214/1‑94 (MQ=60)
                                    CAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTAT                                                           >  SRR4302540.5467569/1‑94 (MQ=60)
                                     AGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATG                                                          <  SRR4302540.2967840/94‑1 (MQ=60)
                                     AGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATG                                                          >  SRR4302540.2767090/1‑94 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         <  SRR4302540.7001839/94‑1 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         >  SRR4302540.9067670/1‑94 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         <  SRR4302540.5741850/94‑1 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         <  SRR4302540.7165661/94‑1 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         <  SRR4302540.3218268/94‑1 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         >  SRR4302540.3568540/1‑94 (MQ=60)
                                      GGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGC                                                         >  SRR4302540.8183687/1‑94 (MQ=60)
                                       GGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCG                                                        >  SRR4302540.8976979/1‑94 (MQ=60)
                                       GGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCG                                                        >  SRR4302540.7118684/1‑94 (MQ=60)
                                        GTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTTGCCTATGCGT                                                       >  SRR4302540.1627184/1‑94 (MQ=60)
                                        GTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGT                                                       >  SRR4302540.1594180/1‑94 (MQ=60)
                                         TTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTT                                                      >  SRR4302540.6296298/1‑94 (MQ=60)
                                         TTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTT                                                      <  SRR4302540.7771368/94‑1 (MQ=60)
                                         TTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTT                                                      <  SRR4302540.4123036/94‑1 (MQ=60)
                                          TAATTTGGCAATTATGCTTGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTC                                                     <  SRR4302540.9086781/94‑1 (MQ=60)
                                          TAATTTGGCAATTATGCATGCTGACCATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGCCCTAAAACTGTCCCCGTTTCGCCTATGCGTTC                                                     <  SRR4302540.7387152/94‑1 (MQ=60)
                                          TAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTC                                                     >  SRR4302540.8921467/1‑94 (MQ=60)
                                          TAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTC                                                     <  SRR4302540.4388453/94‑1 (MQ=60)
                                            ATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTC                                                   <  SRR4302540.9227730/94‑1 (MQ=60)
                                            ATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTC                                                   >  SRR4302540.6976415/1‑94 (MQ=60)
                                             TTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCT                                                  >  SRR4302540.262145/1‑94 (MQ=60)
                                             TTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCT                                                  <  SRR4302540.2384492/94‑1 (MQ=60)
                                               TGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTC                                                <  SRR4302540.7178812/94‑1 (MQ=60)
                                               TGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTC                                                <  SRR4302540.930748/94‑1 (MQ=60)
                                               TGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTC                                                >  SRR4302540.6806783/1‑94 (MQ=60)
                                               TAGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTC                                                <  SRR4302540.5582783/94‑1 (MQ=60)
                                                GGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCC                                               >  SRR4302540.963692/1‑94 (MQ=60)
                                                 GCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCA                                              <  SRR4302540.8609130/94‑1 (MQ=60)
                                                   AATTATGCATGCTGAACATTCTTCTAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACC                                            >  SRR4302540.8555104/1‑94 (MQ=60)
                                                    ATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCA                                           >  SRR4302540.1361376/1‑94 (MQ=60)
                                                    ATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCA                                           >  SRR4302540.1403464/1‑94 (MQ=60)
                                                    ATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCA                                           >  SRR4302540.9584387/1‑94 (MQ=60)
                                                     TTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCAT                                          >  SRR4302540.1457050/1‑94 (MQ=60)
                                                     TTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCAT                                          <  SRR4302540.441832/94‑1 (MQ=60)
                                                     TTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCAT                                          >  SRR4302540.5317483/1‑94 (MQ=60)
                                                      TATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATT                                         >  SRR4302540.4198484/1‑94 (MQ=60)
                                                      TATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATT                                         >  SRR4302540.9431530/1‑94 (MQ=60)
                                                       ATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTT                                        <  SRR4302540.9480540/94‑1 (MQ=60)
                                                        TGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTC                                       <  SRR4302540.7781544/94‑1 (MQ=60)
                                                         GCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCC                                      >  SRR4302540.7705276/1‑94 (MQ=60)
                                                         GCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCC                                      <  SRR4302540.456991/94‑1 (MQ=60)
                                                          CATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCG                                     >  SRR4302540.3452351/1‑94 (MQ=60)
                                                           ATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGT                                    >  SRR4302540.6578941/1‑94 (MQ=60)
                                                           ATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGT                                    >  SRR4302540.2946710/1‑94 (MQ=60)
                                                           ATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGT                                    >  SRR4302540.696232/1‑94 (MQ=60)
                                                            TGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTT                                   >  SRR4302540.8639719/1‑94 (MQ=60)
                                                            TGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGATTCGCCTATGCGTTCTCTTCCACCATTTCCGTT                                   >  SRR4302540.9834316/1‑94 (MQ=60)
                                                             GCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTA                                  >  SRR4302540.1759822/1‑94 (MQ=60)
                                                              CTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAT                                 >  SRR4302540.3448351/1‑94 (MQ=60)
                                                              CTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAT                                 >  SRR4302540.1507902/1‑94 (MQ=60)
                                                              CTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAT                                 <  SRR4302540.845811/94‑1 (MQ=60)
                                                              CTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAT                                 <  SRR4302540.6282591/94‑1 (MQ=60)
                                                               TGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATG                                >  SRR4302540.9934413/1‑94 (MQ=60)
                                                               TGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATG                                >  SRR4302540.4591423/1‑94 (MQ=60)
                                                                 AACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTG                              <  SRR4302540.3904652/94‑1 (MQ=60)
                                                                 AACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTG                              <  SRR4302540.122474/94‑1 (MQ=60)
                                                                  ACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGC                             <  SRR4302540.5778021/94‑1 (MQ=60)
                                                                  ACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGC                             <  SRR4302540.4062108/94‑1 (MQ=60)
                                                                  ACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGC                             >  SRR4302540.6844081/1‑94 (MQ=60)
                                                                  ACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGC                             <  SRR4302540.3943031/94‑1 (MQ=60)
                                                                   CATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCA                            >  SRR4302540.10154070/1‑94 (MQ=60)
                                                                    ATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAA                           >  SRR4302540.6434107/1‑94 (MQ=60)
                                                                    ATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAA                           >  SRR4302540.3639852/1‑94 (MQ=60)
                                                                    ATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAA                           >  SRR4302540.3171359/1‑94 (MQ=60)
                                                                     TTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAAC                          <  SRR4302540.6571538/94‑1 (MQ=60)
                                                                     TTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAAC                          >  SRR4302540.8499096/1‑94 (MQ=60)
                                                                      TCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACA                         <  SRR4302540.8880913/94‑1 (MQ=60)
                                                                      TCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACA                         <  SRR4302540.6236951/94‑1 (MQ=60)
                                                                       CTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAA                        >  SRR4302540.6149865/1‑94 (MQ=60)
                                                                        TTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAAC                       <  SRR4302540.2297427/94‑1 (MQ=60)
                                                                         TCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACT                      >  SRR4302540.2350024/1‑94 (MQ=60)
                                                                         TCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACT                      <  SRR4302540.5617851/94‑1 (MQ=60)
                                                                          CCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTT                     >  SRR4302540.1466543/1‑94 (MQ=60)
                                                                           CAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTG                    >  SRR4302540.8041466/1‑94 (MQ=60)
                                                                            AGCATNTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTNTCGCCTATGCGTNCTNTTCCACCATTTCCGTTATGTGCAACAACTTGA                   >  SRR4302540.4805801/1‑94 (MQ=60)
                                                                            AGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGA                   >  SRR4302540.8006426/1‑94 (MQ=60)
                                                                            AGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGA                   >  SRR4302540.1780513/1‑94 (MQ=60)
                                                                             GCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCACAAACTTGAT                  >  SRR4302540.366260/1‑94 (MQ=60)
                                                                             GCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAT                  >  SRR4302540.7513482/1‑94 (MQ=60)
                                                                             ACATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAT                  <  SRR4302540.6697167/94‑1 (MQ=60)
                                                                              CATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATT                 >  SRR4302540.6836171/1‑94 (MQ=60)
                                                                               ATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTT                >  SRR4302540.365176/1‑94 (MQ=60)
                                                                               ATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTT                <  SRR4302540.2717594/94‑1 (MQ=60)
                                                                               ATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTT                >  SRR4302540.4244093/1‑94 (MQ=60)
                                                                               ATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTCCAACAACTTGATTT                <  SRR4302540.8677115/94‑1 (MQ=60)
                                                                                TGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTA               <  SRR4302540.2227807/94‑1 (MQ=60)
                                                                                 GTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTAT              <  SRR4302540.7019686/94‑1 (MQ=60)
                                                                                 GTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTAT              <  SRR4302540.6455797/94‑1 (MQ=60)
                                                                                 GTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTGTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTAT              <  SRR4302540.9154832/94‑1 (MQ=60)
                                                                                  TTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATA             <  SRR4302540.1477249/94‑1 (MQ=60)
                                                                                  TTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATA             <  SRR4302540.4972506/94‑1 (MQ=60)
                                                                                    TACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACC           >  SRR4302540.4119582/1‑94 (MQ=60)
                                                                                     ACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCT          >  SRR4302540.6071691/1‑94 (MQ=60)
                                                                                     ACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCT          <  SRR4302540.2120600/94‑1 (MQ=60)
                                                                                     ACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCT          >  SRR4302540.492349/1‑94 (MQ=60)
                                                                                      CTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTA         <  SRR4302540.1696947/94‑1 (MQ=60)
                                                                                      CTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTA         <  SRR4302540.7938531/94‑1 (MQ=60)
                                                                                      CTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTA         <  SRR4302540.6117268/94‑1 (MQ=60)
                                                                                      CTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTA         <  SRR4302540.2820062/94‑1 (MQ=60)
                                                                                      CTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTA         >  SRR4302540.2533181/1‑94 (MQ=60)
                                                                                       TGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAA        <  SRR4302540.5276976/94‑1 (MQ=60)
                                                                                       TGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAA        <  SRR4302540.3358124/94‑1 (MQ=60)
                                                                                       TGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAA        <  SRR4302540.6398094/94‑1 (MQ=60)
                                                                                         TCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA      >  SRR4302540.6665535/1‑94 (MQ=60)
                                                                                         TCACAGGCGTGAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA      <  SRR4302540.8329005/94‑1 (MQ=60)
                                                                                          CACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACAT     <  SRR4302540.2859070/94‑1 (MQ=60)
                                                                                          CACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACAT     <  SRR4302540.8233069/94‑1 (MQ=60)
                                                                                            CAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTT   >  SRR4302540.9113947/1‑94 (MQ=60)
                                                                                            CAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTT   >  SRR4302540.5003215/1‑94 (MQ=60)
                                                                                            CAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTT   <  SRR4302540.4648329/94‑1 (MQ=60)
                                                                                            CAGGCGTTAAGACCTAAAACTGTCACCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTT   >  SRR4302540.4424982/1‑94 (MQ=60)
                                                                                             AGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTG  <  SRR4302540.2064419/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAGACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTG  >  NC_000913/3628666‑3628852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑