Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,638,755 A→G A88A (GCA→GCG)  yhiP → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,638,7550AG99.5% 1291.1 / NA 405A88A (GCA→GCG) yhiPputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (191/212);  total (191/214)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 428 total aligned reads displayed.

TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG  >  NC_000913/3638665‑3638844
                                                                                          |                                                                                         
ttttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCt                                                                                         <  2:2976970/93‑1 (MQ=255)
 tttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCtt                                                                                        >  2:7338525/1‑93 (MQ=255)
  ttGCTGTGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtg                                                                                       <  2:9644161/93‑1 (MQ=255)
  ttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtg                                                                                       <  2:7298708/93‑1 (MQ=255)
  ttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtg                                                                                       <  1:7154320/93‑1 (MQ=255)
    gctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtgtg                                                                                     <  1:9365189/93‑1 (MQ=255)
     ctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCCAACGCACCATTGTTCTTGGAGCGCCTGTGc                                                                                    >  2:3773429/1‑93 (MQ=255)
     ctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGc                                                                                    <  2:6582997/93‑1 (MQ=255)
     ctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGc                                                                                    <  1:8628437/93‑1 (MQ=255)
      tgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                   <  1:4511525/93‑1 (MQ=255)
      tgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                   >  1:189317/1‑93 (MQ=255)
       gcgcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTg                                                                                  <  2:1947187/93‑1 (MQ=255)
        cgcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTgg                                                                                 <  1:6218132/93‑1 (MQ=255)
        cgcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTgg                                                                                 <  1:9577361/93‑1 (MQ=255)
         gcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGc                                                                                >  1:6840130/1‑93 (MQ=255)
            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             <  2:1909824/93‑1 (MQ=255)
            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             >  1:3453297/1‑93 (MQ=255)
            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             <  1:1389622/93‑1 (MQ=255)
             gTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAtt                                                                            <  1:7318544/93‑1 (MQ=255)
              tCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTg                                                                           <  1:832813/93‑1 (MQ=255)
                tATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                         >  2:178421/1‑93 (MQ=255)
                tATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                         >  2:6532730/1‑93 (MQ=255)
                tATGGCCTCACTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                         <  1:4411433/93‑1 (MQ=255)
                 aTGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCt                                                                        >  2:7658330/1‑93 (MQ=255)
                   ggCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTAc                                                                      <  2:221978/93‑1 (MQ=255)
                   ggCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTAc                                                                      >  2:9350988/1‑93 (MQ=255)
                    gCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACt                                                                     >  1:6728306/1‑93 (MQ=255)
                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGTGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGTTGGCGATTGGCTACTTCa                                                                  >  1:3712262/1‑93 (MQ=255)
                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCa                                                                  <  2:3108972/93‑1 (MQ=255)
                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCa                                                                  <  2:5623366/93‑1 (MQ=255)
                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCGTGTGCTGGCGATTGGCTACTTCa                                                                  >  2:5524231/1‑93 (MQ=255)
                        cATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTTCTTCAt                                                                 <  2:3270455/93‑1 (MQ=255)
                        cATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCAt                                                                 >  2:1649974/1‑93 (MQ=255)
                         ctttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                <  1:2750037/92‑1 (MQ=255)
                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                <  2:3495537/93‑1 (MQ=255)
                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                >  2:8905348/1‑93 (MQ=255)
                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                >  2:6382701/1‑93 (MQ=255)
                          tttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGa                                                               <  1:6504785/93‑1 (MQ=255)
                          tttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGa                                                               <  1:408673/93‑1 (MQ=255)
                          tttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAAAGCATCATTGTTCTTGGCGCGCTTGTGCTGGCGATTGGCTACTTCATgc                                                               >  2:1158880/1‑92 (MQ=255)
                           ttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGAc                                                              <  1:5160417/93‑1 (MQ=255)
                             ccATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCg                                                            >  1:5877603/1‑93 (MQ=255)
                              cATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCgg                                                           <  2:4063042/93‑1 (MQ=255)
                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                         <  2:4284966/93‑1 (MQ=255)
                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                         >  2:2311266/1‑93 (MQ=255)
                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                         >  1:2551361/1‑93 (MQ=255)
                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCCTGTGCTGGCGATTGGCTACTTCATGACCCGCa                                                         >  1:1669476/1‑93 (MQ=255)
                                 tGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCAt                                                        >  2:912674/1‑93 (MQ=255)
                                   gcggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGt                                                      <  2:4486783/93‑1 (MQ=255)
                                      gttATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGc                                                   <  1:5819056/91‑1 (MQ=255)
                                      ggtATGTCGGCGACCCCCTGCTGGGGACCAAACTCCCCAGTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGc                                                   <  1:7513432/91‑1 (MQ=255)
                                        tATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTa                                                 >  1:3964347/1‑93 (MQ=255)
                                         aTGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAc                                                >  2:9518833/1‑93 (MQ=255)
                                          tGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACt                                               >  2:2762830/1‑93 (MQ=255)
                                          tGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACt                                               >  1:5126066/1‑93 (MQ=255)
                                            tCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTa                                             <  1:3143664/93‑1 (MQ=255)
                                             cGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTaa                                            <  1:6933000/93‑1 (MQ=255)
                                               gCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGc                                          >  1:1010490/1‑93 (MQ=255)
                                                 gACCACCTGCTGGGGACCATACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcct                                        <  1:2265083/93‑1 (MQ=255)
                                                 gACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcct                                        <  1:8073053/93‑1 (MQ=255)
                                                  accaccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctg                                       >  2:5044082/1‑93 (MQ=255)
                                                   ccaccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctga                                      <  1:4755580/93‑1 (MQ=255)
                                                     cccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacc                                    <  2:12373/92‑1 (MQ=255)
                                                     accTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacc                                    >  1:9645806/1‑93 (MQ=255)
                                                       ctgctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacctg                                  >  2:415613/1‑93 (MQ=255)
                                                        tgctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacctga                                 >  2:746065/1‑93 (MQ=255)
                                                        tgctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacctga                                 <  1:4622555/93‑1 (MQ=255)
                                                           tgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGGATGTCGCTACTTAAGCCTGACCTGAttt                              >  1:9956746/1‑93 (MQ=255)
                                                           tgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAttt                              <  1:8501894/93‑1 (MQ=255)
                                                              ggACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCa                           <  2:2512219/93‑1 (MQ=255)
                                                               gACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAGTTTCAt                          >  2:2437147/1‑93 (MQ=255)
                                                                aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATc                         >  1:1199072/1‑93 (MQ=255)
                                                                aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATc                         >  2:7942529/1‑93 (MQ=255)
                                                                aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATc                         <  1:390553/93‑1 (MQ=255)
                                                                aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATc                         >  1:2426440/1‑93 (MQ=255)
                                                                 ccAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCg                        <  2:6792165/93‑1 (MQ=255)
                                                                  cAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGc                       >  2:5258225/1‑93 (MQ=255)
                                                                   aaaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGcc                      >  2:5382135/1‑93 (MQ=255)
                                                                   aaaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGcc                      <  2:3709611/93‑1 (MQ=255)
                                                                   aaaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGcc                      <  2:7733889/93‑1 (MQ=255)
                                                                   aaaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGcc                      >  2:5778548/1‑93 (MQ=255)
                                                                     aCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCt                    <  2:4795437/93‑1 (MQ=255)
                                                                       gCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTgg                  >  2:8327306/1‑93 (MQ=255)
                                                                        cACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTCCTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg                 <  1:3557797/93‑1 (MQ=255)
                                                                        cACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg                 >  1:7875228/1‑93 (MQ=255)
                                                                        cACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg                 <  2:714514/93‑1 (MQ=255)
                                                                          ccATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGa               >  2:1331132/1‑93 (MQ=255)
                                                                          ccATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGa               <  1:5318777/93‑1 (MQ=255)
                                                                           cATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAc              <  2:6068301/93‑1 (MQ=255)
                                                                           cATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAc              <  1:1038390/93‑1 (MQ=255)
                                                                            aTTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACt             <  2:1525346/93‑1 (MQ=255)
                                                                              tGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAt           <  2:6161229/93‑1 (MQ=255)
                                                                              tGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAt           <  1:9765928/93‑1 (MQ=255)
                                                                                ttCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCg         >  1:7720555/1‑93 (MQ=255)
                                                                                ttCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGGCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCg         >  2:8141952/1‑93 (MQ=255)
                                                                                   ttGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGTGACTATCGCTg      >  2:8765536/1‑93 (MQ=255)
                                                                                   ttGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTg      <  2:290438/93‑1 (MQ=255)
                                                                                    tGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGt     <  1:2305932/93‑1 (MQ=255)
                                                                                     ggAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTc    <  1:5987456/93‑1 (MQ=255)
                                                                                      gAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCg   >  1:8832069/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG  >  NC_000913/3638665‑3638844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 222 total aligned reads displayed.
TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  >  NC_000913/3638662‑3638848
                                                                                             |                                                                                             
TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCG                                                                                               >  SRR4302540.4098685/1‑94 (MQ=60)
TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCG                                                                                               <  SRR4302540.5071453/94‑1 (MQ=60)
TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCG                                                                                               <  SRR4302540.5755765/94‑1 (MQ=60)
 GCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGC                                                                                              >  SRR4302540.2781114/1‑94 (MQ=60)
 GCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGC                                                                                              >  SRR4302540.7525508/1‑94 (MQ=60)
 GCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGC                                                                                              >  SRR4302540.3486785/1‑94 (MQ=60)
  CTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGCCCACCTGCTGGGGACCAAACGCCCCATTGTTCTTGGAGCGCT                                                                                             >  SRR4302540.1688583/1‑94 (MQ=60)
  CTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCT                                                                                             >  SRR4302540.2321551/1‑94 (MQ=60)
   TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTT                                                                                            <  SRR4302540.9856176/94‑1 (MQ=60)
     TTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGT                                                                                          <  SRR4302540.7234055/94‑1 (MQ=60)
     TTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGT                                                                                          <  SRR4302540.138799/94‑1 (MQ=60)
       GCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGC                                                                                        <  SRR4302540.9476703/94‑1 (MQ=60)
        GTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       <  SRR4302540.8728660/94‑1 (MQ=60)
        CTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       <  SRR4302540.8728661/94‑1 (MQ=60)
        CTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       >  SRR4302540.191202/1‑94 (MQ=60)
        CTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       >  SRR4302540.9917207/1‑94 (MQ=60)
        CTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       <  SRR4302540.2419424/94‑1 (MQ=60)
         TGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTG                                                                                      <  SRR4302540.4560406/94‑1 (MQ=60)
          GCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGG                                                                                     <  SRR4302540.7069863/94‑1 (MQ=60)
           CGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGC                                                                                    <  SRR4302540.9690469/94‑1 (MQ=60)
           CGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGC                                                                                    <  SRR4302540.6287314/94‑1 (MQ=60)
           CGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGC                                                                                    >  SRR4302540.6915891/1‑94 (MQ=60)
            GCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCG                                                                                   <  SRR4302540.514203/94‑1 (MQ=60)
            GCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCG                                                                                   <  SRR4302540.6998371/94‑1 (MQ=60)
              TGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAT                                                                                 >  SRR4302540.3490870/1‑94 (MQ=60)
              TGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAT                                                                                 <  SRR4302540.1227806/94‑1 (MQ=60)
              TGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAT                                                                                 >  SRR4302540.6325466/1‑94 (MQ=60)
              TGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAT                                                                                 >  SRR4302540.6737241/1‑94 (MQ=60)
               GGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATT                                                                                <  SRR4302540.1404491/94‑1 (MQ=60)
               GGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATT                                                                                <  SRR4302540.1102363/94‑1 (MQ=60)
                GTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTG                                                                               <  SRR4302540.6125736/94‑1 (MQ=60)
                GTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTG                                                                               <  SRR4302540.7400285/94‑1 (MQ=60)
                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAAGGCACCAGTGGTCTTGGAGCGCTTGGGCTGGCGACTGG                                                                              >  SRR4302540.9227516/1‑94 (MQ=60)
                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              >  SRR4302540.7988884/1‑94 (MQ=60)
                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              <  SRR4302540.4514588/94‑1 (MQ=60)
                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              <  SRR4302540.127377/94‑1 (MQ=60)
                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              <  SRR4302540.841657/94‑1 (MQ=60)
                  CTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGC                                                                             >  SRR4302540.3993641/1‑94 (MQ=60)
                  CTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGC                                                                             >  SRR4302540.5202962/1‑94 (MQ=60)
                   TATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCTCCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCT                                                                            >  SRR4302540.3716294/1‑94 (MQ=60)
                   TATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCT                                                                            >  SRR4302540.10078648/1‑94 (MQ=60)
                   TATGGCCTCACTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCT                                                                            <  SRR4302540.4459250/94‑1 (MQ=60)
                    ATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTA                                                                           >  SRR4302540.10073263/1‑94 (MQ=60)
                     TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTAC                                                                          <  SRR4302540.5128297/94‑1 (MQ=60)
                     TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTAC                                                                          <  SRR4302540.3583447/94‑1 (MQ=60)
                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         >  SRR4302540.6802593/1‑94 (MQ=60)
                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         <  SRR4302540.1728185/94‑1 (MQ=60)
                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         >  SRR4302540.4778813/1‑94 (MQ=60)
                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACCCACCATGGTTCTTGGAGCACTTGTGCTCGCGATTCGCTTCT                                                                         >  SRR4302540.5346069/1‑94 (MQ=60)
                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGAACAAACGGACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         >  SRR4302540.7507930/1‑94 (MQ=60)
                        CCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTC                                                                       <  SRR4302540.1947586/94‑1 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGGGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGGTTGGCTACTTCA                                                                      >  SRR4302540.4668764/1‑94 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGTGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGTTGGCGATTGGCTACTTCA                                                                      >  SRR4302540.3752772/1‑94 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCA                                                                      <  SRR4302540.1917584/94‑1 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCA                                                                      >  SRR4302540.3778215/1‑94 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCA                                                                      >  SRR4302540.816296/1‑94 (MQ=60)
                         CTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCA                                                                      >  SRR4302540.9364885/1‑94 (MQ=60)
                          TCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCAT                                                                     <  SRR4302540.9140050/94‑1 (MQ=60)
                           CATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATG                                                                    <  SRR4302540.9673217/94‑1 (MQ=60)
                            CTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGA                                                                   <  SRR4302540.2779756/94‑1 (MQ=60)
                            ATTTCCATTGGCGGCTATGTCGGCGACCGCCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGA                                                                   <  SRR4302540.5186699/94‑1 (MQ=60)
                            ATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGA                                                                   <  SRR4302540.179999/94‑1 (MQ=60)
                             TTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGAC                                                                  >  SRR4302540.5894067/1‑94 (MQ=60)
                              TTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACC                                                                 <  SRR4302540.4722169/94‑1 (MQ=60)
                              TTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACC                                                                 <  SRR4302540.5216512/94‑1 (MQ=60)
                              TTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACC                                                                 >  SRR4302540.9950392/1‑94 (MQ=60)
                              TTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACC                                                                 <  SRR4302540.1443092/94‑1 (MQ=60)
                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                <  SRR4302540.3248909/94‑1 (MQ=60)
                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                >  SRR4302540.788919/1‑94 (MQ=60)
                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                >  SRR4302540.7541219/1‑94 (MQ=60)
                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                >  SRR4302540.8519098/1‑94 (MQ=60)
                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                >  SRR4302540.5942569/1‑94 (MQ=60)
                                CCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGG                                                               >  SRR4302540.5514454/1‑94 (MQ=60)
                                 CATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGC                                                              <  SRR4302540.2510355/94‑1 (MQ=60)
                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             >  SRR4302540.2578861/1‑94 (MQ=60)
                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             >  SRR4302540.7127250/1‑94 (MQ=60)
                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCCTGTGCTGGCGATTGGCTACTTCATGACCCGCA                                                             >  SRR4302540.1687641/1‑94 (MQ=60)
                                   TTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCAT                                                            <  SRR4302540.1684999/94‑1 (MQ=60)
                                    TGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATG                                                           >  SRR4302540.3962506/1‑94 (MQ=60)
                                     GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGT                                                          >  SRR4302540.716741/1‑94 (MQ=60)
                                     GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGT                                                          <  SRR4302540.4467992/94‑1 (MQ=60)
                                        GGCTATGTCGGCGACCACCTGCTGGGGTCCAAACGCACCATTGGTCTTGGAGCGCTTGTGCTGGCGCTTGGCTACTTCATGACCGGtattgcgc                                                       >  SRR4302540.6493953/1‑86 (MQ=60)
                                        GGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGC                                                       >  SRR4302540.5666668/1‑94 (MQ=60)
                                        GGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGC                                                       >  SRR4302540.8623877/1‑94 (MQ=60)
                                        GGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGC                                                       >  SRR4302540.2448288/1‑94 (MQ=60)
                                         GGTATGTCGGCGACCCCCTGCTGGGGACCAAACTCCCCAGTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCT                                                      <  SRR4302540.7597432/94‑1 (MQ=60)
                                         GCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCT                                                      <  SRR4302540.7597433/94‑1 (MQ=60)
                                          CTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTA                                                     >  SRR4302540.4007094/1‑94 (MQ=60)
                                           TATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAC                                                    <  SRR4302540.6605445/94‑1 (MQ=60)
                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   <  SRR4302540.5982195/94‑1 (MQ=60)
                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   <  SRR4302540.1031183/94‑1 (MQ=60)
                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   <  SRR4302540.1031182/94‑1 (MQ=60)
                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   >  SRR4302540.5181585/1‑94 (MQ=60)
                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   >  SRR4302540.3962746/1‑94 (MQ=60)
                                              GTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTA                                                 >  SRR4302540.2360990/1‑94 (MQ=60)
                                               TCGGCGGCCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAA                                                <  SRR4302540.2325617/94‑1 (MQ=60)
                                               TCGGCGACCACCTGGTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAA                                                <  SRR4302540.3614776/94‑1 (MQ=60)
                                               TCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAA                                                <  SRR4302540.3177927/94‑1 (MQ=60)
                                               TCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAA                                                <  SRR4302540.9629556/94‑1 (MQ=60)
                                                CGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAG                                               <  SRR4302540.8760648/94‑1 (MQ=60)
                                                CGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAG                                               <  SRR4302540.7010151/94‑1 (MQ=60)
                                                 GGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGC                                              >  SRR4302540.5829952/1‑94 (MQ=60)
                                                 GGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGC                                              <  SRR4302540.1071850/94‑1 (MQ=60)
                                                 GGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGC                                              >  SRR4302540.1020736/1‑94 (MQ=60)
                                                   GGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGGTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCT                                            <  SRR4302540.8501333/94‑1 (MQ=60)
                                                   CGACCGCCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCT                                            <  SRR4302540.2389338/94‑1 (MQ=60)
                                                   CGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCT                                            <  SRR4302540.8501332/94‑1 (MQ=60)
                                                   CGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCT                                            <  SRR4302540.6171955/94‑1 (MQ=60)
                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           >  SRR4302540.5890427/1‑94 (MQ=60)
                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           <  SRR4302540.6893592/94‑1 (MQ=60)
                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           <  SRR4302540.3519974/94‑1 (MQ=60)
                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           <  SRR4302540.8166728/94‑1 (MQ=60)
                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           <  SRR4302540.3298767/94‑1 (MQ=60)
                                                      CCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGAC                                         <  SRR4302540.4806321/94‑1 (MQ=60)
                                                      CCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGAC                                         >  SRR4302540.5078064/1‑94 (MQ=60)
                                                       CACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACC                                        >  SRR4302540.9759406/1‑94 (MQ=60)
                                                       CACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACC                                        >  SRR4302540.2729556/1‑94 (MQ=60)
                                                        ACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT                                       >  SRR4302540.2312269/1‑94 (MQ=60)
                                                        ACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT                                       >  SRR4302540.4323807/1‑94 (MQ=60)
                                                        ACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT                                       >  SRR4302540.2697572/1‑94 (MQ=60)
                                                         CCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTG                                      <  SRR4302540.4401010/94‑1 (MQ=60)
                                                          CTGCTGGGGAGCAAACGCACCATTGTTCTTGGAGCGGTTGTGCTGGCGATTGGCTACTTtagtaccggcaggccgctgcttacgccgggccgaa                                     >  SRR4302540.8923387/1‑59 (MQ=60)
                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302540.4070174/1‑94 (MQ=60)
                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302540.3795926/1‑94 (MQ=60)
                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302540.7741109/1‑94 (MQ=60)
                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302540.9006072/1‑94 (MQ=60)
                                                           TGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAT                                    >  SRR4302540.200946/1‑94 (MQ=60)
                                                           TGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAT                                    <  SRR4302540.4672099/94‑1 (MQ=60)
                                                           TGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAT                                    >  SRR4302540.540427/1‑94 (MQ=60)
                                                            GCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATT                                   <  SRR4302540.5911185/94‑1 (MQ=60)
                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGGATGTCGCTACTTAAGCCTGACCTGATTT                                  >  SRR4302540.10072957/1‑94 (MQ=60)
                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTT                                  >  SRR4302540.4757247/1‑94 (MQ=60)
                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTT                                  <  SRR4302540.4046648/94‑1 (MQ=60)
                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTT                                  >  SRR4302540.10072956/1‑94 (MQ=60)
                                                              TGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTT                                 >  SRR4302540.6674387/1‑94 (MQ=60)
                                                              TGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTT                                 <  SRR4302540.8600690/94‑1 (MQ=60)
                                                              TGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTT                                 <  SRR4302540.3647383/94‑1 (MQ=60)
                                                               GGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTC                                >  SRR4302540.2829978/1‑94 (MQ=60)
                                                               GGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTC                                >  SRR4302540.5295856/1‑94 (MQ=60)
                                                               GGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTACATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTC                                <  SRR4302540.9711608/94‑1 (MQ=60)
                                                                GGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCA                               >  SRR4302540.4672081/1‑94 (MQ=60)
                                                                 GGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAT                              <  SRR4302540.621816/94‑1 (MQ=60)
                                                                 GGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAT                              <  SRR4302540.4042985/94‑1 (MQ=60)
                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGTATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             >  SRR4302540.9809802/1‑94 (MQ=60)
                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             >  SRR4302540.2452579/1‑94 (MQ=60)
                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             <  SRR4302540.5211766/94‑1 (MQ=60)
                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             >  SRR4302540.1211404/1‑94 (MQ=60)
                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             >  SRR4302540.7332259/1‑94 (MQ=60)
                                                                   ACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCG                            >  SRR4302540.464767/1‑94 (MQ=60)
                                                                   ACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCG                            <  SRR4302540.394589/94‑1 (MQ=60)
                                                                     CAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCC                          <  SRR4302540.7121284/94‑1 (MQ=60)
                                                                      AAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCC                         <  SRR4302540.8802297/94‑1 (MQ=60)
                                                                        ACGCACCCTTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTG                       >  SRR4302540.6971831/1‑94 (MQ=60)
                                                                        ACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTG                       <  SRR4302540.8707266/94‑1 (MQ=60)
                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      <  SRR4302540.1510291/94‑1 (MQ=60)
                                                                          GCGCCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGG                     <  SRR4302540.2485896/94‑1 (MQ=60)
                                                                          GCACCATTGTTCTTGGAGCGCTTTTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGG                     <  SRR4302540.9508363/94‑1 (MQ=60)
                                                                          GCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGG                     >  SRR4302540.7967215/1‑94 (MQ=60)
                                                                          GCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGG                     <  SRR4302540.602132/94‑1 (MQ=60)
                                                                           CACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTCCTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGG                    <  SRR4302540.3596667/94‑1 (MQ=60)
                                                                           CACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGG                    >  SRR4302540.7263742/1‑94 (MQ=60)
                                                                            ACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGA                   >  SRR4302540.4394239/1‑94 (MQ=60)
                                                                            ACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGA                   >  SRR4302540.3796488/1‑94 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  >  SRR4302540.8878175/1‑94 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  <  SRR4302540.7973469/94‑1 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  >  SRR4302540.8332647/1‑94 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  >  SRR4302540.4241920/1‑94 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  <  SRR4302540.5377020/94‑1 (MQ=60)
                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  <  SRR4302540.5377019/94‑1 (MQ=60)
                                                                             CCATTATTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  <  SRR4302540.3046782/94‑1 (MQ=60)
                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 >  SRR4302540.6905309/1‑94 (MQ=60)
                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 <  SRR4302540.7578301/94‑1 (MQ=60)
                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 <  SRR4302540.7578302/94‑1 (MQ=60)
                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 >  SRR4302540.6905310/1‑94 (MQ=60)
                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 <  SRR4302540.1048950/94‑1 (MQ=60)
                                                                               ATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTA                >  SRR4302540.8499006/1‑94 (MQ=60)
                                                                               ATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTA                >  SRR4302540.9774989/1‑94 (MQ=60)
                                                                                TTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAT               <  SRR4302540.638432/94‑1 (MQ=60)
                                                                                 TGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATC              <  SRR4302540.9880454/94‑1 (MQ=60)
                                                                                 TGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATC              <  SRR4302540.5994550/94‑1 (MQ=60)
                                                                                  GTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCG             >  SRR4302540.7810109/1‑94 (MQ=60)
                                                                                  GTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCG             <  SRR4302540.327534/94‑1 (MQ=60)
                                                                                  GTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGAATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCG             >  SRR4302540.7810111/1‑94 (MQ=60)
                                                                                    TCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCT           <  SRR4302540.9703615/94‑1 (MQ=60)
                                                                                    TCTTGGAGCGCTTGTGCTGGCGATTGGCTACGTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCT           <  SRR4302540.2746588/94‑1 (MQ=60)
                                                                                     CTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTG          >  SRR4302540.1046680/1‑94 (MQ=60)
                                                                                     CTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTG          >  SRR4302540.6360308/1‑94 (MQ=60)
                                                                                       TGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTC        <  SRR4302540.3000193/94‑1 (MQ=60)
                                                                                       TGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTC        <  SRR4302540.2330749/94‑1 (MQ=60)
                                                                                        GGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCG       <  SRR4302540.6053774/94‑1 (MQ=60)
                                                                                        GGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCG       <  SRR4302540.7806508/94‑1 (MQ=60)
                                                                                         GAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG      <  SRR4302540.6512171/94‑1 (MQ=60)
                                                                                         GAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG      >  SRR4302540.7908571/1‑94 (MQ=60)
                                                                                         GAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG      <  SRR4302540.8354408/94‑1 (MQ=60)
                                                                                          AGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCCCTACTTAGGCTTGACCTGATTTTCATCGCCCTGGGGCCTATCGCTGTCGGA     >  SRR4302540.115687/1‑94 (MQ=60)
                                                                                           GCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTA    >  SRR4302540.7190426/1‑94 (MQ=60)
                                                                                           GCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTA    >  SRR4302540.6038804/1‑94 (MQ=60)
                                                                                           GCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGCCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATTCCTGACGGTA    >  SRR4302540.4244525/1‑94 (MQ=60)
                                                                                            CGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAA   >  SRR4302540.5805465/1‑94 (MQ=60)
                                                                                             GCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  <  SRR4302540.4282598/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  >  NC_000913/3638662‑3638848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑