Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,638,755	A→G	A88A (GCA→GCG)	yhiP →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,638,755	0	A	G	100.0%	747.9 / NA	234	A88A (GCA→GCG)	yhiP	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (112/122); total (112/122)

Only 100 of 237 total aligned reads displayed.

TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG  >  NC_000913/3638665‑3638844

ttttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCt                                                                                         <  1:5777199/93‑1 (MQ=255)

 tttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCtt                                                                                        >  2:5874733/1‑93 (MQ=255)

   tgctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTtgt                                                                                      >  2:4020711/1‑93 (MQ=255)

     ctgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGc                                                                                    <  1:4217628/93‑1 (MQ=255)

      tgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                   <  2:605321/93‑1 (MQ=255)

      tgcGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCt                                                                                   <  2:4376564/93‑1 (MQ=255)

       gcgcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTg                                                                                  <  2:6005662/93‑1 (MQ=255)

         gcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGc                                                                                <  1:1444495/93‑1 (MQ=255)

         gcTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGc                                                                                >  1:1941215/1‑93 (MQ=255)

          cTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCg                                                                               <  1:810834/93‑1 (MQ=255)

           tGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGa                                                                              >  2:424592/1‑93 (MQ=255)

            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             <  2:2809106/93‑1 (MQ=255)

            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             <  1:4825288/93‑1 (MQ=255)

            ggTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAt                                                                             >  2:109513/1‑93 (MQ=255)

             gTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGAtt                                                                            <  2:1735160/93‑1 (MQ=255)

              tCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTg                                                                           <  2:5182439/93‑1 (MQ=255)

              tCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTg                                                                           <  1:1878826/93‑1 (MQ=255)

               cTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTgg                                                                          <  2:2559060/93‑1 (MQ=255)

                tATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                         >  1:4084175/1‑93 (MQ=255)

                tATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGc                                                                         <  2:3736283/93‑1 (MQ=255)

                 aTGGCCTCATTTCCATTGGCGGCTATGTCGGCGAACACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCt                                                                        >  1:245241/1‑93 (MQ=255)

                  tGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTa                                                                       >  2:2139155/1‑93 (MQ=255)

                  tGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTa                                                                       >  2:2249296/1‑93 (MQ=255)

                   ggCCTCATTCCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTAc                                                                      <  2:2419459/93‑1 (MQ=255)

                     ccTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACtt                                                                    <  1:4390777/93‑1 (MQ=255)

                     ccTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACtt                                                                    >  2:2396102/1‑93 (MQ=255)

                      cTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTc                                                                   >  2:3381302/1‑93 (MQ=255)

                      cTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTc                                                                   >  2:4623095/1‑93 (MQ=255)

                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCa                                                                  >  2:716501/1‑93 (MQ=255)

                       tCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCa                                                                  >  2:3349600/1‑93 (MQ=255)

                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                >  1:5441110/1‑93 (MQ=255)

                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                >  2:1787157/1‑93 (MQ=255)

                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                <  1:2829090/93‑1 (MQ=255)

                         aTTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATg                                                                <  2:5249223/93‑1 (MQ=255)

                          tttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGa                                                               >  2:2193625/1‑93 (MQ=255)

                             ccATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCg                                                            <  2:2796618/93‑1 (MQ=255)

                              cATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCgg                                                           >  2:486742/1‑93 (MQ=255)

                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTTATGTCCGGCa                                                         >  2:3469047/1‑93 (MQ=255)

                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                         >  1:2225094/1‑93 (MQ=255)

                                ttGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCa                                                         >  2:2268569/1‑93 (MQ=255)

                                 tGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCAt                                                        <  2:1026633/93‑1 (MQ=255)

                                   gcggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGt                                                      <  1:5291070/93‑1 (MQ=255)

                                   gcggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGt                                                      <  2:4424685/93‑1 (MQ=255)

                                   gcggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGt                                                      >  2:5731591/1‑93 (MQ=255)

                                     ggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCg                                                    >  2:4447101/1‑93 (MQ=255)

                                     ggcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCg                                                    >  1:3389087/1‑93 (MQ=255)

                                      gcTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGc                                                   <  1:363689/93‑1 (MQ=255)

                                         aTGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAc                                                <  1:3462455/93‑1 (MQ=255)

                                         aTGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAc                                                >  1:3517636/1‑93 (MQ=255)

                                         aTGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAc                                                <  2:3073544/93‑1 (MQ=255)

                                         aTGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAc                                                >  2:1379336/1‑93 (MQ=255)

                                           gTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACtt                                              <  1:4952367/93‑1 (MQ=255)

                                             cGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTaa                                            <  2:3567949/93‑1 (MQ=255)

                                               gCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGc                                          <  1:1687151/93‑1 (MQ=255)

                                                  accacgTGCTGGGGACCAAACGCACCATTGCTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGATCGGCATGTCGCTACTTAAGcctg                                       >  2:3023869/1‑93 (MQ=255)

                                                  accaccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctg                                       <  1:5212402/93‑1 (MQ=255)

                                                   ccaccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctga                                      <  1:2099048/93‑1 (MQ=255)

                                                     cccTGCTGGGGACCAAACACACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacc                                    <  2:3222248/92‑1 (MQ=255)

                                                     accTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacc                                    >  1:258562/1‑93 (MQ=255)

                                                      ccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacct                                   <  1:1019110/93‑1 (MQ=255)

                                                      ccTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacct                                   >  1:305732/1‑93 (MQ=255)

                                                        tgctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGcctgacctga                                 >  1:884535/1‑93 (MQ=255)

                                                         gctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAt                                <  1:6170628/93‑1 (MQ=255)

                                                         gctgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAt                                >  1:6061596/1‑93 (MQ=255)

                                                           tgGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAttt                              >  1:389876/1‑93 (MQ=255)

                                                            ggggACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAtttt                             <  2:3544043/93‑1 (MQ=255)

                                                            ggggACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAtttt                             <  1:3821465/93‑1 (MQ=255)

                                                              ggACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCa                           <  1:4839528/93‑1 (MQ=255)

                                                               gACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAt                          <  1:4050689/93‑1 (MQ=255)

                                                                aCCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATc                         <  1:568557/93‑1 (MQ=255)

                                                                  cAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGc                       >  1:2980563/1‑93 (MQ=255)

                                                                  cAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGc                       >  2:1431405/1‑93 (MQ=255)

                                                                    aaCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGccc                     <  1:5121466/93‑1 (MQ=255)

                                                                     aCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCt                    <  1:5549912/93‑1 (MQ=255)

                                                                     aCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCt                    <  2:5360051/93‑1 (MQ=255)

                                                                      cGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                   <  1:4553934/93‑1 (MQ=255)

                                                                      cGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTg                   <  1:402409/93‑1 (MQ=255)

                                                                       gCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTgg                  >  1:118652/1‑93 (MQ=255)

                                                                       gCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTgg                  <  2:2015406/93‑1 (MQ=255)

                                                                       gCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTgg                  >  1:315172/1‑93 (MQ=255)

                                                                        cACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg                 <  1:5565178/93‑1 (MQ=255)

                                                                          ccATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGa               >  1:5928446/1‑93 (MQ=255)

                                                                           cATTGTTCTTGGAGCGCTTGTGCTGGTGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAc              >  1:3272590/1‑93 (MQ=255)

                                                                           cATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAc              >  1:939877/1‑93 (MQ=255)

                                                                             ttGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa            <  2:4002455/93‑1 (MQ=255)

                                                                             ttGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa            >  1:2926092/1‑93 (MQ=255)

                                                                             ttGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTa            >  2:4947279/1‑93 (MQ=255)

                                                                              tGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAt           <  2:1314234/93‑1 (MQ=255)

                                                                              tGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAt           <  2:107639/93‑1 (MQ=255)

                                                                               gTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATc          >  1:2303062/1‑93 (MQ=255)

                                                                               gTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATc          <  1:379813/93‑1 (MQ=255)

                                                                                   ttGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGATTATCGCTg      <  2:6152641/93‑1 (MQ=255)

                                                                                   ttGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTg      <  1:4509527/93‑1 (MQ=255)

                                                                                     ggAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTc    >  1:2043830/1‑93 (MQ=255)

                                                                                     ggAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTc    <  1:2290544/93‑1 (MQ=255)

                                                                                      gAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCg   <  1:3308779/93‑1 (MQ=255)

                                                                                      gAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCg   <  2:5535074/93‑1 (MQ=255)

                                                                                       aGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCgg  >  1:2649936/1‑93 (MQ=255)

                                                                                       aGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCgg  >  2:2331718/1‑93 (MQ=255)

                                                                                       aGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCgg  <  2:868024/93‑1 (MQ=255)

TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG  >  NC_000913/3638665‑3638844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  >  NC_000913/3638662‑3638848

TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCG                                                                                               <  SRR4302533.3476146/94‑1 (MQ=60)

TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCG                                                                                               <  SRR4302533.3669464/94‑1 (MQ=60)

   TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTT                                                                                            <  SRR4302533.5812094/94‑1 (MQ=60)

   TTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTT                                                                                            <  SRR4302533.3808692/94‑1 (MQ=60)

    TTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTG                                                                                           <  SRR4302533.1991665/94‑1 (MQ=60)

    TTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTG                                                                                           >  SRR4302533.1882987/1‑94 (MQ=60)

     TTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGT                                                                                          >  SRR4302533.4075043/1‑94 (MQ=60)

     TTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGT                                                                                          <  SRR4302533.2629420/94‑1 (MQ=60)

        CTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCT                                                                                       <  SRR4302533.4243139/94‑1 (MQ=60)

          GCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGG                                                                                     <  SRR4302533.3325121/94‑1 (MQ=60)

           CGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGC                                                                                    >  SRR4302533.1953053/1‑94 (MQ=60)

            GCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCG                                                                                   <  SRR4302533.1453209/94‑1 (MQ=60)

             CTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGA                                                                                  <  SRR4302533.815588/94‑1 (MQ=60)

              TGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGGT                                                                                 >  SRR4302533.4848276/1‑94 (MQ=60)

               GGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATT                                                                                <  SRR4302533.4854530/94‑1 (MQ=60)

               GGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATT                                                                                <  SRR4302533.4854529/94‑1 (MQ=60)

                GTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAACCGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTG                                                                               <  SRR4302533.486393/94‑1 (MQ=60)

                GTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTG                                                                               >  SRR4302533.3546063/1‑94 (MQ=60)

                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              <  SRR4302533.1890276/94‑1 (MQ=60)

                 TCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGG                                                                              >  SRR4302533.3046040/1‑94 (MQ=60)

                  CTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGT                                                                             >  SRR4302533.2213488/1‑94 (MQ=60)

                  CTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGC                                                                             >  SRR4302533.4109038/1‑94 (MQ=60)

                   TATGGCCTCATTTCCATTGGCGGCTATGTCGGCGAACACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCT                                                                            >  SRR4302533.246760/1‑94 (MQ=60)

                    ATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTA                                                                           >  SRR4302533.3463144/1‑94 (MQ=60)

                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         <  SRR4302533.2815840/94‑1 (MQ=60)

                      GGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACT                                                                         >  SRR4302533.891671/1‑94 (MQ=60)

                        CCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTC                                                                       <  SRR4302533.4417285/94‑1 (MQ=60)

                        CCTCATTTCCATTGGCGGCCATGGCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGGTGGCGATTGGTGACTTC                                                                       >  SRR4302533.1991964/1‑94 (MQ=60)

                          TCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCAT                                                                     <  SRR4302533.558369/94‑1 (MQ=60)

                           CATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGTTACTTCATG                                                                    >  SRR4302533.432164/1‑94 (MQ=60)

                           CATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATG                                                                    <  SRR4302533.2593723/94‑1 (MQ=60)

                           CATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATG                                                                    >  SRR4302533.5473880/1‑94 (MQ=60)

                            ATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGA                                                                   <  SRR4302533.2846496/94‑1 (MQ=60)

                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                <  SRR4302533.4958099/94‑1 (MQ=60)

                               TCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCG                                                                <  SRR4302533.2015616/94‑1 (MQ=60)

                                 CATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGC                                                              >  SRR4302533.5307468/1‑94 (MQ=60)

                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             >  SRR4302533.2238833/1‑94 (MQ=60)

                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             <  SRR4302533.5826194/94‑1 (MQ=60)

                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             >  SRR4302533.5146718/1‑94 (MQ=60)

                                  ATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCA                                                             >  SRR4302533.6001835/1‑94 (MQ=60)

                                   TTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCAT                                                            >  SRR4302533.2393829/1‑94 (MQ=60)

                                    TGGCGGCTGTGTCGGCGCCCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATG                                                           <  SRR4302533.3270852/94‑1 (MQ=60)

                                      GCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTC                                                         <  SRR4302533.5322752/94‑1 (MQ=60)

                                      GCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTC                                                         <  SRR4302533.520263/94‑1 (MQ=60)

                                      GCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTC                                                         <  SRR4302533.5482512/94‑1 (MQ=60)

                                      GCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTC                                                         <  SRR4302533.6210029/94‑1 (MQ=60)

                                       CGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCG                                                        >  SRR4302533.1637656/1‑94 (MQ=60)

                                       CGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCG                                                        >  SRR4302533.3410015/1‑94 (MQ=60)

                                        GGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGC                                                       >  SRR4302533.3863648/1‑94 (MQ=60)

                                         GCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCT                                                      <  SRR4302533.365898/94‑1 (MQ=60)

                                          CTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTA                                                     >  SRR4302533.822661/1‑94 (MQ=60)

                                           TATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTAC                                                    >  SRR4302533.3539322/1‑94 (MQ=60)

                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   >  SRR4302533.3517186/1‑94 (MQ=60)

                                            ATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACT                                                   <  SRR4302533.3483829/94‑1 (MQ=60)

                                              GTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTA                                                 <  SRR4302533.4982361/94‑1 (MQ=60)

                                               TCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAA                                                <  SRR4302533.5860622/94‑1 (MQ=60)

                                                CGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAG                                               <  SRR4302533.3748123/94‑1 (MQ=60)

                                                 GGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGC                                              >  SRR4302533.3413264/1‑94 (MQ=60)

                                                  GCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCC                                             <  SRR4302533.1697375/94‑1 (MQ=60)

                                                    GACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTG                                           <  SRR4302533.3043190/94‑1 (MQ=60)

                                                     ACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGA                                          <  SRR4302533.2407467/94‑1 (MQ=60)

                                                     ACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGA                                          <  SRR4302533.5243792/94‑1 (MQ=60)

                                                     ACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGA                                          >  SRR4302533.2988237/1‑94 (MQ=60)

                                                      CCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGAC                                         <  SRR4302533.2111998/94‑1 (MQ=60)

                                                       CACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACC                                        >  SRR4302533.260135/1‑94 (MQ=60)

                                                        ACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT                                       <  SRR4302533.3827478/94‑1 (MQ=60)

                                                        ACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT                                       >  SRR4302533.307601/1‑94 (MQ=60)

                                                         CCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTG                                      <  SRR4302533.4985872/94‑1 (MQ=60)

                                                         CCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTG                                      >  SRR4302533.1383130/1‑94 (MQ=60)

                                                         CCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTG                                      <  SRR4302533.1025099/94‑1 (MQ=60)

                                                         CCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTG                                      >  SRR4302533.1924282/1‑94 (MQ=60)

                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302533.889731/1‑94 (MQ=60)

                                                          CTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGA                                     >  SRR4302533.2895592/1‑94 (MQ=60)

                                                           TGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAT                                    >  SRR4302533.2753180/1‑94 (MQ=60)

                                                           TGCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGAT                                    >  SRR4302533.6098291/1‑94 (MQ=60)

                                                            GCTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATT                                   <  SRR4302533.6207713/94‑1 (MQ=60)

                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTT                                  >  SRR4302533.392231/1‑94 (MQ=60)

                                                             CTGGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTT                                  <  SRR4302533.2276026/94‑1 (MQ=60)

                                                               GGGGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTC                                <  SRR4302533.3844832/94‑1 (MQ=60)

                                                                 GGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAT                              <  SRR4302533.4868861/94‑1 (MQ=60)

                                                                 GGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAT                              <  SRR4302533.6150227/94‑1 (MQ=60)

                                                                 GGACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCAT                              >  SRR4302533.3226457/1‑94 (MQ=60)

                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             <  SRR4302533.3371093/94‑1 (MQ=60)

                                                                  GACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATC                             <  SRR4302533.4075365/94‑1 (MQ=60)

                                                                   ACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCG                            <  SRR4302533.763773/94‑1 (MQ=60)

                                                                   ACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCG                            <  SRR4302533.4107346/94‑1 (MQ=60)

                                                                   ACCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCG                            <  SRR4302533.571919/94‑1 (MQ=60)

                                                                    CCAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGC                           >  SRR4302533.2998798/1‑94 (MQ=60)

                                                                     CAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCC                          >  SRR4302533.4437776/1‑94 (MQ=60)

                                                                     CAAACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCC                          <  SRR4302533.1481907/94‑1 (MQ=60)

                                                                       AACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCT                        <  SRR4302533.5405565/94‑1 (MQ=60)

                                                                       AACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCT                        <  SRR4302533.5152409/94‑1 (MQ=60)

                                                                        ACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTG                       <  SRR4302533.5583428/94‑1 (MQ=60)

                                                                        ACGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTG                       <  SRR4302533.39942/94‑1 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      >  SRR4302533.2311279/1‑94 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      >  SRR4302533.317095/1‑94 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      >  SRR4302533.119406/1‑94 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      <  SRR4302533.3090662/94‑1 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      <  SRR4302533.404834/94‑1 (MQ=60)

                                                                         CGCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGG                      <  SRR4302533.4581553/94‑1 (MQ=60)

                                                                          GCACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGG                     >  SRR4302533.115312/1‑94 (MQ=60)

                                                                           CACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGG                    >  SRR4302533.4078317/1‑94 (MQ=60)

                                                                           CACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGG                    <  SRR4302533.5598816/94‑1 (MQ=60)

                                                                            ACCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGA                   >  SRR4302533.5964280/1‑94 (MQ=60)

                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGTGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  >  SRR4302533.3292788/1‑94 (MQ=60)

                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  >  SRR4302533.945399/1‑94 (MQ=60)

                                                                             CCATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGAC                  <  SRR4302533.5749238/94‑1 (MQ=60)

                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 >  SRR4302533.5325245/1‑94 (MQ=60)

                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 <  SRR4302533.2266890/94‑1 (MQ=60)

                                                                              CATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACT                 >  SRR4302533.4013128/1‑94 (MQ=60)

                                                                               ATTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTA                >  SRR4302533.2943995/1‑94 (MQ=60)

                                                                                TTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAT               >  SRR4302533.4119887/1‑94 (MQ=60)

                                                                                TTGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTAT               >  SRR4302533.4564250/1‑94 (MQ=60)

                                                                                 TGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATC              <  SRR4302533.5667254/94‑1 (MQ=60)

                                                                                 TGTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATC              >  SRR4302533.2317330/1‑94 (MQ=60)

                                                                                  GTTCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCG             <  SRR4302533.382101/94‑1 (MQ=60)

                                                                                    TCTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGGCCTGATTTTCATCGCCCTGGGGACTATCGCT           >  SRR4302533.2100828/1‑94 (MQ=60)

                                                                                     CTTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTG          >  SRR4302533.5643838/1‑94 (MQ=60)

                                                                                      TTGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGT         <  SRR4302533.4536839/94‑1 (MQ=60)

                                                                                       TGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTC        >  SRR4302533.2056430/1‑94 (MQ=60)

                                                                                       TGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTC        >  SRR4302533.977959/1‑94 (MQ=60)

                                                                                       TGGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTC        <  SRR4302533.4443244/94‑1 (MQ=60)

                                                                                        GGAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCG       <  SRR4302533.2304729/94‑1 (MQ=60)

                                                                                         GAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG      >  SRR4302533.2666214/1‑94 (MQ=60)

                                                                                         GAGCGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGG      <  SRR4302533.3329208/94‑1 (MQ=60)

                                                                                            CGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAA   <  SRR4302533.2283862/94‑1 (MQ=60)

                                                                                            CGCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAA   >  SRR4302533.4621543/1‑94 (MQ=60)

                                                                                             GCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  <  SRR4302533.3510531/94‑1 (MQ=60)

                                                                                             GCTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  <  SRR4302533.1692190/94‑1 (MQ=60)

TGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAAC  >  NC_000913/3638662‑3638848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`