Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,4360CG99.8% 1318.1 / NA 417N87K (AAC→AAG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (184/232);  total (185/232)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,714,4370GC99.8% 1327.8 / NA 417V88L (GTG→CTG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (184/232);  minor base T (1/0);  total (185/232)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 432 total aligned reads displayed.

TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523
                                                                                       ||                                                                                      
tCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcg                                                                                    <  1:8628079/93‑1 (MQ=255)
 cgcgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcgc                                                                                   >  1:8477399/1‑93 (MQ=255)
   cgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGa                                                                                 >  2:6129649/1‑93 (MQ=255)
        aaGGCGCGCTGATTGGCGGAGCAGCAGGCGCAGCTCGGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:3456385/93‑1 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:6917149/93‑1 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  2:8438721/93‑1 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:6247909/93‑1 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            >  2:5239/1‑93 (MQ=255)
         aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt                                                                           <  1:9232698/93‑1 (MQ=255)
         aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt                                                                           >  2:3037565/1‑93 (MQ=255)
          ggCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          <  1:2787869/93‑1 (MQ=255)
          ggCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          <  2:9548237/93‑1 (MQ=255)
          ggCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          >  1:5127483/1‑93 (MQ=255)
            cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  1:7607403/93‑1 (MQ=255)
             gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  2:8207995/93‑1 (MQ=255)
             gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  2:7608859/93‑1 (MQ=255)
              cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      >  1:1543054/1‑93 (MQ=255)
                cTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc                                                                    >  1:3256161/1‑93 (MQ=255)
                 tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa                                                                   <  2:9875519/93‑1 (MQ=255)
                  gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc                                                                  >  2:2624878/1‑93 (MQ=255)
                      ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              <  2:3235218/93‑1 (MQ=255)
                       gcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtg                                                             <  1:6725777/93‑1 (MQ=255)
                        cgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt                                                            <  1:7502662/93‑1 (MQ=255)
                           agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         >  2:9266459/1‑93 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:2237666/93‑1 (MQ=255)
                            acagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:3521653/92‑1 (MQ=255)
                             cagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg                                                       <  1:1001670/93‑1 (MQ=255)
                              agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      >  2:7133966/1‑93 (MQ=255)
                              agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      <  1:5763650/93‑1 (MQ=255)
                              agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      >  1:3649596/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:7056599/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:1420240/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:7747737/1‑93 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     <  2:1493611/93‑1 (MQ=255)
                               gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     <  1:1755520/93‑1 (MQ=255)
                                 agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc                                                   >  2:9339105/1‑93 (MQ=255)
                                  ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  >  1:7999393/1‑93 (MQ=255)
                                    cgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg                                                >  2:5621474/1‑93 (MQ=255)
                                     gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc                                               <  1:5979269/93‑1 (MQ=255)
                                     gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc                                               >  2:7103367/1‑93 (MQ=255)
                                     gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc                                               >  1:9639042/1‑93 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  1:4049526/93‑1 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              >  1:9042786/1‑93 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              >  2:3692268/1‑93 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTCTTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:5557773/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:189973/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:9751442/93‑1 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            <  1:504584/93‑1 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  2:2772556/1‑93 (MQ=255)
                                         ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           >  1:6643019/1‑93 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  2:2940646/93‑1 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          >  2:1150136/1‑93 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  2:3589135/93‑1 (MQ=255)
                                           ctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg                                         <  2:4477667/93‑1 (MQ=255)
                                            tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        <  1:1164540/93‑1 (MQ=255)
                                            tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        <  1:1409758/93‑1 (MQ=255)
                                            tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        <  1:5934876/93‑1 (MQ=255)
                                            cgggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        <  1:7306319/92‑1 (MQ=255)
                                             gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  1:4663040/93‑1 (MQ=255)
                                              ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  1:7103259/93‑1 (MQ=255)
                                                cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    <  2:4550981/93‑1 (MQ=255)
                                                cggcgccgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    <  1:3901561/93‑1 (MQ=255)
                                                 ggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc                                   >  2:4218424/1‑93 (MQ=255)
                                                  gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  1:8316365/93‑1 (MQ=255)
                                                   cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAt                                 <  2:49220/93‑1 (MQ=255)
                                                    ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                >  2:4737527/1‑93 (MQ=255)
                                                     gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               <  2:24870/93‑1 (MQ=255)
                                                        ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            <  2:7354920/93‑1 (MQ=255)
                                                          tgTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  2:2018356/92‑1 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  1:1929325/93‑1 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          >  2:633996/1‑93 (MQ=255)
                                                             tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       >  2:3835169/1‑93 (MQ=255)
                                                             tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       >  2:5660849/1‑93 (MQ=255)
                                                             tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       >  1:3758747/1‑93 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGTGCTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  1:4234861/93‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  2:8056856/93‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  1:4531505/93‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      >  1:2365442/1‑93 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  1:8894333/93‑1 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  2:4092513/93‑1 (MQ=255)
                                                                taCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCCATATg                    >  2:10040925/1‑93 (MQ=255)
                                                                  cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGcc                  <  2:9127776/93‑1 (MQ=255)
                                                                  cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGcc                  >  2:8581708/1‑93 (MQ=255)
                                                                  cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAACATGcc                  >  1:2829197/1‑93 (MQ=255)
                                                                    tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                >  1:4979155/1‑93 (MQ=255)
                                                                    tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                >  1:2030847/1‑93 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATTCCGAACaa            <  2:5013912/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  2:4027042/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  2:7209215/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            >  2:6507735/1‑93 (MQ=255)
                                                                          tgCAGGAAGCGACGCTGCGCGACACAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  2:8938748/93‑1 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          >  2:5947560/1‑93 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  1:614046/93‑1 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  2:1704479/93‑1 (MQ=255)
                                                                            cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgtg        >  1:9509174/1‑93 (MQ=255)
                                                                             aGGAAGCGAAGCTGCGCGACAAAATGCGCTGCTCTGGTGTTAGCGTAACCCGCAGCGGGCATAACATTATCCTCAATATGCCGAACAATGTGa       <  1:5262087/93‑1 (MQ=255)
                                                                              ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  1:6455579/1‑93 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  1:2998326/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  >  1:9885392/1‑93 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  1:7739013/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 225 total aligned reads displayed.
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525
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GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302540.3469474/94‑1 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      <  SRR4302540.8703334/94‑1 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      >  SRR4302540.9091553/1‑94 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      >  SRR4302540.7301085/1‑94 (MQ=60)
      TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGC                                                                                     >  SRR4302540.8575860/1‑94 (MQ=60)
      TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGC                                                                                     <  SRR4302540.8728298/94‑1 (MQ=60)
      TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGGTATTACATGGATGTGCAGGAAGCGAAGCTGCGC                                                                                     >  SRR4302540.7594117/1‑94 (MQ=60)
        GCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGA                                                                                   >  SRR4302540.2020071/1‑94 (MQ=60)
         CGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAC                                                                                  >  SRR4302540.8875484/1‑94 (MQ=60)
         CGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAC                                                                                  >  SRR4302540.7065551/1‑94 (MQ=60)
         CGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCACGAAGCGAAGCTGCGCGAC                                                                                  >  SRR4302540.7065552/1‑94 (MQ=60)
          GGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACA                                                                                 >  SRR4302540.9602239/1‑94 (MQ=60)
          GGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACA                                                                                 >  SRR4302540.4155242/1‑94 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTGTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              >  SRR4302540.1831972/1‑94 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              >  SRR4302540.7723282/1‑94 (MQ=60)
              AAGGCGCGCTGATTGGCGGAGCAGCAGGCGCAGCTCGGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302540.3494004/94‑1 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302540.6994120/94‑1 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302540.6317441/94‑1 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302540.8962767/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302540.9343350/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302540.2831677/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302540.5385043/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            >  SRR4302540.5183016/1‑94 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           <  SRR4302540.9731453/94‑1 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           >  SRR4302540.3207384/1‑94 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           <  SRR4302540.2817976/94‑1 (MQ=60)
                 GCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCG                                                                          <  SRR4302540.2836060/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         <  SRR4302540.849288/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         <  SRR4302540.7692891/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         <  SRR4302540.371992/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         >  SRR4302540.3715550/1‑94 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         <  SRR4302540.9186403/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        >  SRR4302540.1560093/1‑94 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       >  SRR4302540.10018961/1‑94 (MQ=60)
                     GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      >  SRR4302540.3291652/1‑94 (MQ=60)
                      CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCCGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                     >  SRR4302540.6899510/1‑94 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    <  SRR4302540.6563117/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    <  SRR4302540.5161817/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    <  SRR4302540.2821736/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302540.4472868/1‑94 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    <  SRR4302540.8755566/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302540.9476551/1‑94 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGGGGGTTAGTACAAGGATGTGCAGGAAGCGAAGTTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302540.9030144/1‑94 (MQ=60)
                        GATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACT                                                                   <  SRR4302540.9749097/94‑1 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  <  SRR4302540.5012726/94‑1 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  >  SRR4302540.6027201/1‑94 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  >  SRR4302540.9701450/1‑94 (MQ=60)
                          TTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGG                                                                 >  SRR4302540.7597759/1‑94 (MQ=60)
                           TGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                <  SRR4302540.5554902/94‑1 (MQ=60)
                             GCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGT                                                              <  SRR4302540.6800021/94‑1 (MQ=60)
                              CGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTT                                                             <  SRR4302540.7586503/94‑1 (MQ=60)
                               GCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTA                                                            <  SRR4302540.7251855/94‑1 (MQ=60)
                               GCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTA                                                            >  SRR4302540.9897243/1‑94 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302540.2261731/94‑1 (MQ=60)
                                  ACAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302540.3560036/94‑1 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        <  SRR4302540.1011848/94‑1 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        >  SRR4302540.3689576/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302540.3899221/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302540.3823704/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302540.4823734/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       <  SRR4302540.5827287/94‑1 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       <  SRR4302540.600279/94‑1 (MQ=60)
                                     GCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAA                                                      <  SRR4302540.1774543/94‑1 (MQ=60)
                                     GCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAA                                                      <  SRR4302540.3968276/94‑1 (MQ=60)
                                      CAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAC                                                     <  SRR4302540.1708120/94‑1 (MQ=60)
                                      CAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAC                                                     <  SRR4302540.5007119/94‑1 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302540.6971910/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302540.8092504/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302540.3396564/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGAGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302540.649227/1‑94 (MQ=60)
                                       AGGCGAAGCTCTGGGCGGCGTCGTTGGCTATTACATGGATGTGCAGAAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    <  SRR4302540.1093234/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302540.10018926/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302540.3367058/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGAGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302540.1757987/94‑1 (MQ=60)
                                          CGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGC                                                 >  SRR4302540.9752594/1‑94 (MQ=60)
                                          CGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGC                                                 >  SRR4302540.7832574/1‑94 (MQ=60)
                                          CGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGC                                                 <  SRR4302540.5205747/94‑1 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGTGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                <  SRR4302540.5249139/94‑1 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                >  SRR4302540.9151331/1‑94 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                <  SRR4302540.6045466/94‑1 (MQ=60)
                                            GAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCCAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302540.2280846/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302540.9871941/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302540.4093184/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302540.8078148/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302540.2428290/94‑1 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              <  SRR4302540.7128700/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             <  SRR4302540.7723305/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             <  SRR4302540.4441608/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             <  SRR4302540.10087705/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             >  SRR4302540.923296/1‑94 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             <  SRR4302540.509986/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             >  SRR4302540.6716522/1‑94 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            >  SRR4302540.4643933/1‑94 (MQ=60)
                                                 CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGG                                          <  SRR4302540.8362465/94‑1 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         <  SRR4302540.1176623/94‑1 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         <  SRR4302540.1424943/94‑1 (MQ=60)
                                                  CGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         <  SRR4302540.7387886/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        <  SRR4302540.148050/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        <  SRR4302540.4712954/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        >  SRR4302540.10110383/1‑94 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        <  SRR4302540.289670/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302540.283335/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302540.2008431/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302540.7182289/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302540.8456234/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302540.6758714/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302540.217625/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302540.4593244/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     >  SRR4302540.1094776/1‑94 (MQ=60)
                                                      CGGCGCCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302540.3943887/94‑1 (MQ=60)
                                                       ggttgggtttGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTGAGCGTAACCCGCAGCGGGGATAACA                                    <  SRR4302540.6623003/84‑1 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    <  SRR4302540.6623002/94‑1 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    >  SRR4302540.527210/1‑94 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGGGTTAGCGTAACCCGCAGCGGGGATAACT                                    >  SRR4302540.6200195/1‑94 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302540.5904509/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302540.645758/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   >  SRR4302540.1171023/1‑94 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302540.8412907/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302540.8412906/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAAAT                                   >  SRR4302540.8660867/1‑94 (MQ=60)
                                                         CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                  <  SRR4302540.1811268/94‑1 (MQ=60)
                                                          GGTGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302540.8177669/94‑1 (MQ=60)
                                                          GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302540.9353675/94‑1 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                <  SRR4302540.5883730/94‑1 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302540.4750438/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302540.6700203/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302540.2265232/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302540.2265233/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               <  SRR4302540.3623886/94‑1 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAACCTGCGCGACAAAATGCGAGGCACTGGTGCTAGCGTAACCCGCAGCGGGGAGCACATTATC                               >  SRR4302540.8673843/1‑94 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              <  SRR4302540.6506349/94‑1 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              <  SRR4302540.6254595/94‑1 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              >  SRR4302540.1275503/1‑94 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              >  SRR4302540.3434510/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             <  SRR4302540.6272524/94‑1 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302540.7416655/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302540.6133101/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302540.2074119/1‑94 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                           <  SRR4302540.1950351/94‑1 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                           >  SRR4302540.8014218/1‑94 (MQ=60)
                                                                 GTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAA                          >  SRR4302540.1680254/1‑94 (MQ=60)
                                                                 GTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGGGTTAGCGTCACCCGCAGCGGGGCTAACATTCTCCTCAA                          >  SRR4302540.3293404/1‑94 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         >  SRR4302540.3799749/1‑94 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         >  SRR4302540.5869804/1‑94 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302540.5559756/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302540.2200596/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         >  SRR4302540.3799747/1‑94 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302540.7234071/94‑1 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302540.4067461/94‑1 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        >  SRR4302540.2390912/1‑94 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302540.3137118/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGTGCTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302540.4280943/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302540.4580553/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       >  SRR4302540.6195345/1‑94 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302540.1528219/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302540.8997289/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302540.4034201/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      >  SRR4302540.5179931/1‑94 (MQ=60)
                                                                       ACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAACATGCC                    >  SRR4302540.2859794/1‑94 (MQ=60)
                                                                        CATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCG                   >  SRR4302540.6900034/1‑94 (MQ=60)
                                                                         ATGGATGTGTAGGAAGCGAAGATGCGCGACAAAATGCGCCGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  <  SRR4302540.9319952/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  >  SRR4302540.5033050/1‑94 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  >  SRR4302540.2052767/1‑94 (MQ=60)
                                                                           GGATGTGCAGGAAGCGAAGCTGCGCGACACAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                <  SRR4302540.4214239/94‑1 (MQ=60)
                                                                           GGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                <  SRR4302540.7438658/94‑1 (MQ=60)
                                                                             ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA              >  SRR4302540.6398223/1‑94 (MQ=60)
                                                                             ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA              >  SRR4302540.4162122/1‑94 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302540.6740206/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302540.1603168/94‑1 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG            >  SRR4302540.2103124/1‑94 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG            <  SRR4302540.6640800/94‑1 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG            >  SRR4302540.2994413/1‑94 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302540.3145097/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302540.763513/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302540.620598/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302540.8825936/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           >  SRR4302540.1192843/1‑94 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          >  SRR4302540.9621817/1‑94 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          <  SRR4302540.2451638/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          <  SRR4302540.3522872/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          >  SRR4302540.2051820/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCGATATGCCGAACAATGTGA         >  SRR4302540.3510279/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         >  SRR4302540.3510566/1‑94 (MQ=60)
                                                                                  CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         >  SRR4302540.5049674/1‑94 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCTGCTCTGGTGTTAGCGTAACCCGCAGCGGGCATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302540.5319495/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302540.368527/94‑1 (MQ=60)
                                                                                    GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC       <  SRR4302540.513410/94‑1 (MQ=60)
                                                                                    GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC       <  SRR4302540.3795672/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      >  SRR4302540.6315284/1‑94 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302540.7615554/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302540.8301507/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302540.5066149/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302540.6037670/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302540.3031358/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    >  SRR4302540.10000815/1‑94 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302540.4421721/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302540.7829361/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302540.1321445/94‑1 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302540.782774/1‑94 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  <  SRR4302540.7211210/94‑1 (MQ=60)
                                                                                             |                                                                                         
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑