Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,4360CG100.0% 729.6 / NA 231N87K (AAC→AAG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (86/145);  total (86/145)
*NC_0009133,714,4370GC100.0% 730.5 / NA 231V88L (GTG→CTG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (86/145);  total (86/145)

Only 100 of 240 total aligned reads displayed.

CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714350‑3714523
                                                                                      ||                                                                                      
  cgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGa                                                                                 <  2:267845/93‑1 (MQ=255)
    gTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACa                                                                               >  2:6032805/1‑93 (MQ=255)
    gTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACa                                                                               >  2:4570663/1‑93 (MQ=255)
     tAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa                                                                              >  1:417833/1‑93 (MQ=255)
     tAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa                                                                              >  1:806978/1‑93 (MQ=255)
      aaaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaa                                                                             <  1:3074634/93‑1 (MQ=255)
       aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:5780632/93‑1 (MQ=255)
        aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt                                                                           <  1:5108775/93‑1 (MQ=255)
         ggCGCGCTGATTGGCGGAGCAGCAGGCGCAGCTCTGGGCGGCGGCGCTGGTTATTACATGGATGTGCAGGGCGCGAAGCTGCGCGACAAGATg                                                                          >  2:3537200/1‑93 (MQ=255)
         ggCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          <  2:832073/93‑1 (MQ=255)
           cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  2:2456971/93‑1 (MQ=255)
           cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  2:1654925/93‑1 (MQ=255)
            gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  2:122803/93‑1 (MQ=255)
            gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  2:3640896/93‑1 (MQ=255)
            gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  1:6077493/93‑1 (MQ=255)
             cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:2601922/93‑1 (MQ=255)
             cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  2:1072157/93‑1 (MQ=255)
               cTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc                                                                    <  2:1318309/93‑1 (MQ=255)
                 gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc                                                                  <  2:3862755/93‑1 (MQ=255)
                 gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc                                                                  >  2:4932931/1‑93 (MQ=255)
                   ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                >  2:2808205/1‑93 (MQ=255)
                   ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                <  2:2999369/93‑1 (MQ=255)
                     ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              <  1:3803510/93‑1 (MQ=255)
                      gcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtg                                                             >  1:1374613/1‑93 (MQ=255)
                       cgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt                                                            <  1:5439260/93‑1 (MQ=255)
                         cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          <  1:3286134/93‑1 (MQ=255)
                          agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         <  1:69410/93‑1 (MQ=255)
                          agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         <  1:4879648/93‑1 (MQ=255)
                           gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:2333080/93‑1 (MQ=255)
                           gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        >  1:946290/1‑93 (MQ=255)
                           gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:1429387/93‑1 (MQ=255)
                             agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCga                                                      >  2:4190313/1‑92 (MQ=255)
                             agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      >  1:5564496/1‑93 (MQ=255)
                             agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      >  1:4635590/1‑93 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  1:3742193/1‑93 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:4742018/1‑93 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  1:3861468/1‑93 (MQ=255)
                                  gcgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc                                                 >  2:5824424/1‑93 (MQ=255)
                                    gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc                                               >  2:642290/1‑93 (MQ=255)
                                     cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  2:3086383/93‑1 (MQ=255)
                                        ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           >  1:3154152/1‑93 (MQ=255)
                                        ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           >  2:2509774/1‑93 (MQ=255)
                                         tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  2:4045704/93‑1 (MQ=255)
                                         tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  1:5699363/93‑1 (MQ=255)
                                           tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        >  2:5361718/1‑93 (MQ=255)
                                            gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       >  1:2099167/1‑93 (MQ=255)
                                            gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  2:3380918/93‑1 (MQ=255)
                                            gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       >  1:795840/1‑93 (MQ=255)
                                            gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  2:4970390/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      >  1:3268648/1‑93 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  2:4890549/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  2:5376341/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      >  1:2523134/1‑93 (MQ=255)
                                               cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    <  1:2993228/93‑1 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  >  2:1034378/1‑93 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  2:390902/93‑1 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  >  2:5197392/1‑93 (MQ=255)
                                                  cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAt                                 <  1:4529654/93‑1 (MQ=255)
                                                   ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  1:1871932/93‑1 (MQ=255)
                                                   ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  2:1608780/93‑1 (MQ=255)
                                                      gTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc                             >  2:5689707/1‑93 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            >  2:3241843/1‑93 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            <  2:3409930/93‑1 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            >  2:2165691/1‑93 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            <  1:367962/93‑1 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            >  2:1167825/1‑93 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            >  2:2093722/1‑93 (MQ=255)
                                                        tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCt                           <  2:1580972/93‑1 (MQ=255)
                                                           ttattaCTTGGATGTGCAGGAAGCGAAGCTGCGCGGCAAAATGGGTGGCACTGGCGTTAGGGTACGCCGCAGCGGGGATAACATTATTCTCaa                        >  2:4007437/1‑93 (MQ=255)
                                                           ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:1103773/93‑1 (MQ=255)
                                                           ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  2:5156860/93‑1 (MQ=255)
                                                           ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  2:1928504/93‑1 (MQ=255)
                                                            tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       <  2:1465191/93‑1 (MQ=255)
                                                                aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGc                   >  2:3946016/1‑93 (MQ=255)
                                                                 cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGcc                  >  1:4229125/1‑93 (MQ=255)
                                                                 cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGcc                  >  2:4347107/1‑93 (MQ=255)
                                                                  ctagATGTGCAGGAAGCGAGGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCTTATCCCGCAGCGGGGATAACATTATCCTCAATATGCCg                 <  2:1406946/90‑1 (MQ=255)
                                                                  aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCg                 >  1:526825/1‑93 (MQ=255)
                                                                   tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                >  1:4706647/1‑93 (MQ=255)
                                                                   tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                <  1:2829920/93‑1 (MQ=255)
                                                                   tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGa                <  1:3542165/93‑1 (MQ=255)
                                                                    ggATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGaa               <  2:3785663/93‑1 (MQ=255)
                                                                    ggATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGaa               <  2:2614665/93‑1 (MQ=255)
                                                                     gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              >  2:1727423/1‑93 (MQ=255)
                                                                     gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              <  1:5505267/93‑1 (MQ=255)
                                                                     gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              >  1:2383591/1‑93 (MQ=255)
                                                                       tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            >  2:5307788/1‑93 (MQ=255)
                                                                        gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAt           <  2:2943891/93‑1 (MQ=255)
                                                                         tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  1:175200/93‑1 (MQ=255)
                                                                          gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgt         <  1:5865649/93‑1 (MQ=255)
                                                                          gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgt         <  2:2580603/93‑1 (MQ=255)
                                                                          gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgt         <  2:2267574/93‑1 (MQ=255)
                                                                           cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgtg        >  1:2799060/1‑93 (MQ=255)
                                                                            aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGa       <  1:4398298/93‑1 (MQ=255)
                                                                             ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAGCCCGCAGCGGCGATAACATTATCCTCAATATGCCGAACAATGTGAc      <  1:4793899/93‑1 (MQ=255)
                                                                              gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     >  2:6172506/1‑93 (MQ=255)
                                                                              gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     <  1:6212442/93‑1 (MQ=255)
                                                                               aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTACCCCGAAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  1:6304970/93‑1 (MQ=255)
                                                                               aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  1:2851833/93‑1 (MQ=255)
                                                                                 gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  2:926168/93‑1 (MQ=255)
                                                                                      ||                                                                                      
CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714350‑3714523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525
                                                                                             |                                                                                         
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           >  SRR4302533.5117605/1‑94 (MQ=60)
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302533.1575560/94‑1 (MQ=60)
       CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCG                                                                                    <  SRR4302533.802810/94‑1 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                >  SRR4302533.811723/1‑94 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                >  SRR4302533.420339/1‑94 (MQ=60)
            TAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAA                                                                               <  SRR4302533.5545309/94‑1 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              <  SRR4302533.3093507/94‑1 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302533.4906982/94‑1 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302533.5815546/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302533.5139645/94‑1 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGGAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           <  SRR4302533.1495242/94‑1 (MQ=60)
                GGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           <  SRR4302533.6141601/94‑1 (MQ=60)
                 GCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCG                                                                          <  SRR4302533.5477115/94‑1 (MQ=60)
                  CGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGC                                                                         <  SRR4302533.2858635/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302533.6114251/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       >  SRR4302533.315802/1‑94 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302533.2617911/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302533.771581/94‑1 (MQ=60)
                     GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      <  SRR4302533.3044026/94‑1 (MQ=60)
                      CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                     <  SRR4302533.2467219/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302533.4375401/1‑94 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302533.3306128/1‑94 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTG                                                               <  SRR4302533.3826784/94‑1 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTG                                                               >  SRR4302533.1382881/1‑94 (MQ=60)
                              CGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTT                                                             >  SRR4302533.2533894/1‑94 (MQ=60)
                              CGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTT                                                             <  SRR4302533.5472018/94‑1 (MQ=60)
                               GCAGCAGGAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTGCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGTCACTGGTGTTA                                                            <  SRR4302533.4242784/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           <  SRR4302533.4017030/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           >  SRR4302533.3568898/1‑94 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           <  SRR4302533.3306419/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          <  SRR4302533.4909211/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          <  SRR4302533.6266505/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          <  SRR4302533.69863/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          >  SRR4302533.951854/1‑94 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302533.2347524/94‑1 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302533.1956560/94‑1 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302533.1438028/94‑1 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        >  SRR4302533.5598130/1‑94 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        >  SRR4302533.4663678/1‑94 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        <  SRR4302533.5595021/94‑1 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302533.3885039/1‑94 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302533.3765143/1‑94 (MQ=60)
                                      CAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAC                                                     <  SRR4302533.3098944/94‑1 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACGAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302533.3768642/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTTTTAGCGCAACC                                                    >  SRR4302533.2390901/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    <  SRR4302533.1959489/94‑1 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    <  SRR4302533.2138361/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302533.3733943/94‑1 (MQ=60)
                                         GCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCG                                                  <  SRR4302533.5334187/94‑1 (MQ=60)
                                          CGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGC                                                 >  SRR4302533.859544/1‑94 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302533.4458029/94‑1 (MQ=60)
                                              GCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCG                                             >  SRR4302533.3173543/1‑94 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302533.2894124/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302533.6026093/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302533.685237/94‑1 (MQ=60)
                                               CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302533.4901753/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           <  SRR4302533.5733783/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           <  SRR4302533.3961047/94‑1 (MQ=60)
                                                 CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGG                                          >  SRR4302533.858590/1‑94 (MQ=60)
                                                 CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGG                                          <  SRR4302533.5774775/94‑1 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         >  SRR4302533.2112118/1‑94 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         >  SRR4302533.800522/1‑94 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        >  SRR4302533.2538679/1‑94 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        <  SRR4302533.3313436/94‑1 (MQ=60)
                                                   GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        >  SRR4302533.3288815/1‑94 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302533.1360874/94‑1 (MQ=60)
                                                    GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302533.2945943/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302533.3011529/94‑1 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    <  SRR4302533.1611776/94‑1 (MQ=60)
                                                       GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    <  SRR4302533.4627033/94‑1 (MQ=60)
                                                        GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302533.5005943/94‑1 (MQ=60)
                                                         CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                  <  SRR4302533.4557084/94‑1 (MQ=60)
                                                          GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302533.1883345/94‑1 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                >  SRR4302533.5838203/1‑94 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                >  SRR4302533.77820/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302533.5838411/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             <  SRR4302533.370188/94‑1 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302533.3753688/1‑94 (MQ=60)
                                                               TGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTC                            <  SRR4302533.4815517/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302533.1110307/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302533.2833199/94‑1 (MQ=60)
                                                                    ATTACATGGATGTACAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302533.5462800/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      <  SRR4302533.3178878/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302533.2814769/94‑1 (MQ=60)
                                                                       ACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCC                    >  SRR4302533.4254693/1‑94 (MQ=60)
                                                                        CATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCG                   >  SRR4302533.529947/1‑94 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  <  SRR4302533.2713430/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  >  SRR4302533.4735172/1‑94 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 >  SRR4302533.4269922/1‑94 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 <  SRR4302533.3877378/94‑1 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 <  SRR4302533.2847334/94‑1 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 <  SRR4302533.3563994/94‑1 (MQ=60)
                                                                           GGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                >  SRR4302533.2398325/1‑94 (MQ=60)
                                                                           GGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                <  SRR4302533.184941/94‑1 (MQ=60)
                                                                           GGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                <  SRR4302533.5286678/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302533.5538486/94‑1 (MQ=60)
                                                                             ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA              <  SRR4302533.611439/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302533.1419769/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302533.4692741/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302533.176305/94‑1 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          >  SRR4302533.2816276/1‑94 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          <  SRR4302533.5901110/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302533.5668860/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302533.4424853/94‑1 (MQ=60)
                                                                                    GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAGCCCGCAGCGGCGATAACATTATCCTCAATATGCCGAACAATGTGACC       <  SRR4302533.4822943/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302533.6249693/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTACCCCGAAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302533.6342690/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     >  SRR4302533.1746818/1‑94 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302533.2869376/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    <  SRR4302533.4684899/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302533.3347846/94‑1 (MQ=60)
                                                                                        GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302533.2538859/94‑1 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302533.2343877/1‑94 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302533.5031863/1‑94 (MQ=60)
                                                                                             |                                                                                         
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑