Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG99.8% 1906.8 / NA 576S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (322/253);  total (322/254)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,734,2650GC99.8% 1901.5 / NA 576S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (321/254);  minor base A (1/0);  total (322/254)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 588 total aligned reads displayed.

GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351
                                                                                       ||                                                                                      
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  2:123718/93‑1 (MQ=255)
   aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                 >  2:6954263/1‑93 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACACTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                >  1:6317529/1‑93 (MQ=255)
      ggCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGcc                                                                              >  2:3468922/1‑93 (MQ=255)
        cgcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                            <  1:5952097/93‑1 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          <  1:7363188/93‑1 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  2:9378618/1‑93 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  2:9234759/1‑93 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         >  1:454979/1‑93 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         >  1:3681973/1‑93 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         >  1:9178778/1‑93 (MQ=255)
            cACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                        <  2:4555319/93‑1 (MQ=255)
            cACCGATTCTTTGACAGAAATAAGCTGGGAGTACCCTTTCACTTTCCCTGCCGCATTGGTACAACGTCCTTTCATGAATTTCATGCCGACCac                                                                        <  2:6773575/93‑2 (MQ=255)
            cACCGAGTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                        <  2:642726/93‑1 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  2:2327038/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       <  2:9556967/93‑1 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  1:5013065/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      >  1:7207019/1‑93 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     >  1:5306635/1‑93 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     <  1:8452969/93‑1 (MQ=255)
                gATTCTTTGACAGAACTACACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACACCGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    <  1:8921338/93‑1 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:98728/1‑93 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  >  1:104506/1‑93 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               <  1:1341038/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTCCATGCATTTCATGCCGAACAGgttgttgtt                                                               >  1:6558213/1‑93 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             <  1:2890103/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  1:8326040/1‑93 (MQ=255)
                           aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                         >  1:3797120/1‑93 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                      <  2:3723969/93‑1 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCAGGCATTTCATGCCGAACAGGTTGTTGTGGTGtcgcgc                                                      >  2:8593256/1‑93 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCCTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                      >  2:7249166/1‑93 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  1:172616/93‑1 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  1:6721162/93‑1 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    >  1:6087597/1‑93 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    >  2:1874979/1‑93 (MQ=255)
                                 aaaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAg                                                   >  1:10001758/1‑93 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 <  2:4987015/93‑1 (MQ=255)
                                    cTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCtt                                                <  2:6171311/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               <  2:5147068/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               >  1:3248834/1‑93 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               >  2:5858644/1‑93 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  1:2674034/1‑93 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             <  1:1446820/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             <  1:7838944/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             <  2:399952/93‑1 (MQ=255)
                                         aGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACg                                           >  2:768718/1‑93 (MQ=255)
                                         aGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACg                                           <  2:9854945/93‑1 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  1:2963209/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     <  1:8211360/93‑1 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     <  1:1205359/93‑1 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  2:1981818/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  2:1590359/1‑93 (MQ=255)
                                                 ttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCaa                                   >  2:5332041/1‑93 (MQ=255)
                                                 ttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCaa                                   >  2:3590940/1‑93 (MQ=255)
                                                  tCACTGTACCTGGCGCATTGGTACAACGTACTTTCATGCCTTGCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  <  1:572148/93‑1 (MQ=255)
                                                   cACTTTACCTGGAGCATTGGGACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCCAcc                                 >  1:342825/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGGGCCAGCTTCGACGTTCCCCAACCg                                >  2:899701/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                >  1:8568203/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                >  1:6266244/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  2:5438503/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAAAGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               <  2:5556248/93‑1 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCACCCGCt                              >  1:2061014/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  2:2951406/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  1:9372486/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  1:162713/1‑93 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             >  2:9723251/1‑93 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             >  2:6094294/1‑93 (MQ=255)
                                                        tACCTGGCTCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCttt                            <  1:4467912/93‑1 (MQ=255)
                                                        tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCttt                            >  1:247750/1‑93 (MQ=255)
                                                        tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCCCttt                            >  2:2561700/1‑93 (MQ=255)
                                                         tccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTc                           <  2:8260875/92‑1 (MQ=255)
                                                          ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          <  1:1896922/93‑1 (MQ=255)
                                                          ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          <  1:3956780/93‑1 (MQ=255)
                                                           cTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                         >  2:9088878/1‑93 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        <  1:5512408/93‑1 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        >  1:216817/1‑93 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        <  2:886525/93‑1 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        <  2:3154833/93‑1 (MQ=255)
                                                             ggCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGTTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgct                       <  1:4125592/93‑1 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    <  1:7076668/93‑1 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    <  2:7485018/93‑1 (MQ=255)
                                                                 cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAg                   >  2:9471461/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 <  2:1095114/93‑1 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  2:2777057/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  2:6555488/1‑93 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               >  1:7644836/1‑93 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               <  1:4788147/93‑1 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGGTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               <  2:4665832/93‑1 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             <  1:2355518/93‑1 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            <  1:3087490/93‑1 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            <  1:4328517/93‑1 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            >  1:5876895/1‑93 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCGAtcgtcg          >  1:9499012/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         >  2:7935773/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         <  1:357190/93‑1 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca       <  2:4303736/93‑1 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTTCCGAACTGCTTTCTGCTGCACCCATCGTCGccac      >  2:1429767/1‑93 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccac      >  2:4178293/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    <  2:5704823/93‑1 (MQ=255)
                                                                                  ttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATa  <  1:7945008/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 322 total aligned reads displayed.
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302540.5563871/94‑1 (MQ=60)
      GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTC                                                                                     <  SRR4302540.10096348/94‑1 (MQ=60)
       ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    <  SRR4302540.3970081/94‑1 (MQ=60)
       ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    >  SRR4302540.4908066/1‑94 (MQ=60)
        CATAGGCGCTCACCTTTTTTTTGACAGAACTAAGTTGTGATTCCCCTTTCACTTTACCGGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302540.6742183/94‑1 (MQ=44)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302540.7629485/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302540.789432/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302540.4560720/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302540.1651061/94‑1 (MQ=60)
         ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  >  SRR4302540.3662677/1‑94 (MQ=60)
         ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  >  SRR4302540.8015157/1‑94 (MQ=60)
         ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  >  SRR4302540.2502499/1‑94 (MQ=60)
         ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  <  SRR4302540.1305797/94‑1 (MQ=60)
           AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                <  SRR4302540.4591376/94‑1 (MQ=60)
             GCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGA                                                                              <  SRR4302540.272161/94‑1 (MQ=60)
              ccgTCACCGAGTCTTTGACAGAACGAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             <  SRR4302540.6017925/91‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             <  SRR4302540.5446803/94‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             <  SRR4302540.1598848/94‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             >  SRR4302540.3072101/1‑94 (MQ=60)
               GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCTCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            <  SRR4302540.4506598/94‑1 (MQ=60)
               GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            <  SRR4302540.8149691/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302540.7445291/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           >  SRR4302540.3722214/1‑94 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302540.8855377/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302540.8855378/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302540.2952429/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           >  SRR4302540.9288865/1‑94 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTTCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302540.6371328/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302540.1508381/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302540.9468554/94‑1 (MQ=60)
                  CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302540.5067340/1‑94 (MQ=60)
                  CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302540.576733/1‑94 (MQ=60)
                  CACCGATTCTGTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         <  SRR4302540.9736926/94‑1 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        <  SRR4302540.4229244/94‑1 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        >  SRR4302540.7287360/1‑94 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        >  SRR4302540.8479361/1‑94 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        <  SRR4302540.6665457/94‑1 (MQ=60)
                    CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302540.4015156/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302540.267303/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302540.8551108/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302540.3324515/94‑1 (MQ=60)
                      GATTCTTTGACAGAACTACACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACACCGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302540.9024763/94‑1 (MQ=60)
                      GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302540.590164/94‑1 (MQ=60)
                       ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302540.4531358/1‑94 (MQ=60)
                        TTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTG                                                                   >  SRR4302540.6069181/1‑94 (MQ=60)
                        TTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTG                                                                   <  SRR4302540.4754816/94‑1 (MQ=60)
                         TCTTTGACAGAACTATACTGTGAGTACCCTTTCACTTTACCTTGCGCATTGGTACAACGTCCTTTCATGCACTTCATGACCAACAGGTTGTTGT                                                                  >  SRR4302540.5229340/1‑94 (MQ=60)
                         TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  >  SRR4302540.3363158/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302540.6468313/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302540.4063925/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTCCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302540.6630926/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302540.3522949/1‑94 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302540.1355084/94‑1 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302540.5982942/94‑1 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302540.1355083/94‑1 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTAGTACAACGTCCTTTCATGCAGTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302540.6783906/94‑1 (MQ=60)
                            TTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGT                                                               >  SRR4302540.6024805/1‑94 (MQ=60)
                            TTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGT                                                               >  SRR4302540.6538675/1‑94 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302540.4094727/94‑1 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302540.2921592/94‑1 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              >  SRR4302540.3933655/1‑94 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302540.2352187/94‑1 (MQ=60)
                              GACAGAACTAAACTTTGAGTACCGTGTCACTTTACCTGGCGCATTGGTACACCGTCCTTTCATGCCTTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302540.4787653/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302540.1810272/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302540.9333734/94‑1 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302540.2739658/1‑94 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            <  SRR4302540.6299288/94‑1 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302540.737188/1‑94 (MQ=60)
                                CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           >  SRR4302540.3838498/1‑94 (MQ=60)
                                 AGAACTAAACTGTGAGGACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302540.3242761/1‑94 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         <  SRR4302540.8358969/94‑1 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         >  SRR4302540.8241537/1‑94 (MQ=60)
                                   AACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGC                                                        >  SRR4302540.9191512/1‑94 (MQ=60)
                                    ACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCC                                                       >  SRR4302540.3177421/1‑94 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302540.6795339/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302540.174337/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302540.174338/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302540.2074637/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      >  SRR4302540.2326073/1‑94 (MQ=60)
                                     ATAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302540.7308271/94‑1 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACACCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     >  SRR4302540.6353988/1‑94 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     >  SRR4302540.10118271/1‑94 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     >  SRR4302540.10147111/1‑94 (MQ=60)
                                        AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   <  SRR4302540.5076637/94‑1 (MQ=60)
                                        AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   <  SRR4302540.2921718/94‑1 (MQ=60)
                                          CTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTC                                                 >  SRR4302540.3284221/1‑94 (MQ=60)
                                          CTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTC                                                 >  SRR4302540.4790402/1‑94 (MQ=60)
                                           TGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCG                                                <  SRR4302540.3006632/94‑1 (MQ=60)
                                            GTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               <  SRR4302540.6976876/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTTCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302540.7930537/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302540.1462554/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302540.7930532/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302540.7210767/94‑1 (MQ=60)
                                             GGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302540.7210768/94‑1 (MQ=60)
                                               AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            <  SRR4302540.8384795/94‑1 (MQ=60)
                                               AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            <  SRR4302540.5842990/94‑1 (MQ=60)
                                               AGTACCCTTTCACTTTACCTCGCGCATTGGTACAACGTCCTTTCATGAATTTAATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            >  SRR4302540.9750013/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGCCGTT                                           >  SRR4302540.7615087/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           >  SRR4302540.1187471/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           <  SRR4302540.5973648/94‑1 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           >  SRR4302540.1978929/1‑94 (MQ=60)
                                                 TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302540.1972982/1‑94 (MQ=60)
                                                 TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302540.907133/1‑94 (MQ=60)
                                                 TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302540.2995894/1‑94 (MQ=60)
                                                 GACCCTTTCCCTTTGCCGGGCGCATTTGTACAACGTCCTTTCATCCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          <  SRR4302540.842547/94‑1 (MQ=60)
                                                  ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302540.9562372/1‑94 (MQ=60)
                                                  ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302540.9562374/1‑94 (MQ=60)
                                                  ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302540.3098617/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302540.6508232/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302540.700754/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        <  SRR4302540.3792920/94‑1 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302540.1122906/1‑94 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302540.8317453/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      <  SRR4302540.5608556/94‑1 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      >  SRR4302540.5976749/1‑94 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAA                                     >  SRR4302540.239203/1‑94 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAA                                     >  SRR4302540.2397163/1‑94 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAA                                     >  SRR4302540.2534491/1‑94 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    >  SRR4302540.9206128/1‑94 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    >  SRR4302540.10005786/1‑94 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    <  SRR4302540.991154/94‑1 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    >  SRR4302540.5797965/1‑94 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    <  SRR4302540.2247684/94‑1 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    >  SRR4302540.4447857/1‑94 (MQ=60)
                                                       TTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAC                                    >  SRR4302540.489826/1‑94 (MQ=60)
                                                        TCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACC                                   >  SRR4302540.7717823/1‑94 (MQ=60)
                                                        TCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAAC                                   >  SRR4302540.346303/1‑94 (MQ=60)
                                                        TCACTTTACCTGGAGCATTGGGACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCCACC                                   >  SRR4302540.346302/1‑94 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302540.2537102/1‑94 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302540.6335958/1‑94 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTACCCAACCG                                  >  SRR4302540.5287433/1‑94 (MQ=60)
                                                          ACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGC                                 >  SRR4302540.3682679/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCACCCGCT                                >  SRR4302540.2083193/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302540.1876387/94‑1 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302540.9484074/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302540.7743082/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302540.322537/1‑94 (MQ=60)
                                                            TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               <  SRR4302540.2114819/94‑1 (MQ=60)
                                                            TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               >  SRR4302540.6211678/1‑94 (MQ=60)
                                                             TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              >  SRR4302540.250146/1‑94 (MQ=60)
                                                             TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              >  SRR4302540.8658911/1‑94 (MQ=60)
                                                             TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302540.8519600/94‑1 (MQ=60)
                                                              TACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTC                             <  SRR4302540.1153688/94‑1 (MQ=60)
                                                              TACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTC                             <  SRR4302540.9292062/94‑1 (MQ=60)
                                                              TACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTC                             >  SRR4302540.1386374/1‑94 (MQ=60)
                                                               ACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCT                            >  SRR4302540.8569870/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           >  SRR4302540.8806127/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           >  SRR4302540.4368472/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           >  SRR4302540.5409317/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302540.1480634/94‑1 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302540.1917677/94‑1 (MQ=60)
                                                                 CTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGC                          >  SRR4302540.218930/1‑94 (MQ=60)
                                                                  TGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCT                         >  SRR4302540.6875286/1‑94 (MQ=60)
                                                                  TGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCT                         <  SRR4302540.5573121/94‑1 (MQ=60)
                                                                  TGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCT                         <  SRR4302540.4452434/94‑1 (MQ=60)
                                                                   GGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGTTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTG                        <  SRR4302540.4170161/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      <  SRR4302540.43032/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      <  SRR4302540.4591410/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      >  SRR4302540.8443574/1‑94 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      >  SRR4302540.6326634/1‑94 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     >  SRR4302540.7402226/1‑94 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     <  SRR4302540.7155416/94‑1 (MQ=60)
                                                                       CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGC                    >  SRR4302540.5522530/1‑94 (MQ=60)
                                                                       CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGC                    >  SRR4302540.864093/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302540.1240477/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302540.6383427/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302540.5874981/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 >  SRR4302540.7730884/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 >  SRR4302540.5630028/1‑94 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                >  SRR4302540.2124093/1‑94 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302540.8181008/94‑1 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302540.3506687/94‑1 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302540.3880537/94‑1 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302540.4839341/94‑1 (MQ=60)
                                                                           GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                >  SRR4302540.1105013/1‑94 (MQ=60)
                                                                            GTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCG               >  SRR4302540.1711007/1‑94 (MQ=60)
                                                                            GTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGATTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCT               >  SRR4302540.6104988/1‑94 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302540.1037061/1‑94 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302540.2890974/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302540.4375638/94‑1 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302540.3121366/94‑1 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302540.795109/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCGATCGTCG            >  SRR4302540.9611574/1‑94 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGC           >  SRR4302540.1675443/1‑94 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCAGGCTTGGGGGTTCGCCAACGGCTTTCTGGTGGAGCCATCGTCGC           >  SRR4302540.2968692/1‑94 (MQ=32)
                                                                                 ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          <  SRR4302540.360863/94‑1 (MQ=60)
                                                                                  CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCA         >  SRR4302540.7180559/1‑94 (MQ=60)
                                                                                  CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCA         >  SRR4302540.3727632/1‑94 (MQ=60)
                                                                                   GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302540.8339315/1‑94 (MQ=60)
                                                                                   GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302540.1452990/1‑94 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302540.3662686/94‑1 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302540.3986027/94‑1 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     >  SRR4302540.2406969/1‑94 (MQ=60)
                                                                                       TTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA    <  SRR4302540.1275587/94‑1 (MQ=60)
                                                                                        TTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   <  SRR4302540.8939982/94‑1 (MQ=60)
                                                                                        TTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   <  SRR4302540.8037612/94‑1 (MQ=60)
                                                                                        TTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   >  SRR4302540.6213087/1‑94 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302540.3793882/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302540.5998560/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCAAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302540.5161551/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑