Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG100.0% 876.9 / NA 266S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (157/109);  total (157/109)
*NC_0009133,734,2650GC99.6% 866.9 / NA 266S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (156/109);  minor base T (1/0);  total (157/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351
                                                                                       ||                                                                                      
  cATAGGCGGTCACCGATTCTTTGACAGAACTAAACTGTGAGTATCCTTTCACTTTATCTGCCGCAGTGGTACAACGTTCTTTCATGCAtttga                                                                                  >  2:5824536/1‑91 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  2:4033904/93‑1 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  1:3018971/93‑1 (MQ=255)
   aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTTATGCATTTCAt                                                                                 >  1:1571056/1‑93 (MQ=255)
   aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                 <  2:1070680/93‑1 (MQ=255)
     aGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGc                                                                               <  1:1405165/93‑1 (MQ=255)
     aGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGc                                                                               <  1:921674/93‑1 (MQ=255)
       gcgcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCg                                                                             >  2:5541885/1‑93 (MQ=255)
        cgcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                            >  2:2833980/1‑93 (MQ=255)
         gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGaa                                                                           >  1:5868703/1‑93 (MQ=255)
         gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGaa                                                                           >  1:4772398/1‑93 (MQ=255)
         gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGaa                                                                           >  2:3913802/1‑93 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  2:1291555/1‑93 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          <  2:5845192/93‑1 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  1:5539087/93‑1 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  1:5356145/93‑1 (MQ=255)
            cACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGTCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                        >  1:2659673/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       <  2:3261682/93‑1 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  2:4129421/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  1:1028053/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      >  1:1329016/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      <  2:4292764/93‑1 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTTAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     >  1:1890188/1‑93 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     <  2:786091/93‑1 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    <  1:3696514/93‑1 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:5772998/1‑93 (MQ=255)
                 aTTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgt                                                                   <  1:1243308/93‑1 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  <  2:476806/93‑1 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  >  1:1113533/1‑93 (MQ=255)
                   tCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttg                                                                 >  1:2166117/1‑93 (MQ=255)
                    cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgt                                                                <  1:604288/93‑1 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             <  2:364164/93‑1 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             <  2:1194662/93‑1 (MQ=255)
                        taCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  1:4850044/92‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  1:3995548/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  2:5910239/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  2:3213360/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  1:5753168/93‑1 (MQ=255)
                         aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGttgttgttgttg                                                           <  1:3801396/93‑1 (MQ=255)
                          cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                          <  1:5013717/93‑1 (MQ=255)
                           aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                         >  2:2358431/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  2:1970144/93‑1 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                      >  1:2337427/1‑93 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                      <  1:1658433/93‑1 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     >  2:2765908/1‑93 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCCTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  1:3385344/93‑1 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    <  1:2930923/93‑1 (MQ=255)
                                 aaaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAg                                                   >  2:1637019/1‑93 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 <  1:4247292/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               >  2:1799945/1‑93 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  2:4554240/1‑93 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  1:3632027/1‑93 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  1:3507661/1‑93 (MQ=255)
                                        gAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAc                                            >  2:74957/1‑93 (MQ=255)
                                        gAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAc                                            >  2:4393227/1‑93 (MQ=255)
                                        gAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAc                                            >  1:2473323/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          <  2:3008952/93‑1 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          >  2:875145/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          <  2:3037789/93‑1 (MQ=255)
                                           tACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGtt                                         >  2:973151/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  1:5237798/1‑93 (MQ=255)
                                             cccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcc                                       <  2:2437525/93‑1 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcgc                                      >  2:4930280/1‑91 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      >  1:2267/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  2:3808422/1‑93 (MQ=255)
                                                tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                    >  2:6178320/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  >  2:2544967/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  <  2:3064094/93‑1 (MQ=255)
                                                    ccTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                <  2:4278041/92‑1 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               <  1:3838732/93‑1 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  1:6044365/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  2:5325673/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGCCGTTCCCCAACCGCt                              >  2:5272346/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              <  2:2180742/93‑1 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  2:6032631/1‑93 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             <  2:5896076/93‑1 (MQ=255)
                                                          ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          >  1:3909513/1‑93 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        >  1:6160470/1‑93 (MQ=255)
                                                              gcgcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgctg                      <  2:2027824/93‑1 (MQ=255)
                                                                  aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                  <  1:4871801/93‑1 (MQ=255)
                                                                  aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                  <  2:3411740/93‑1 (MQ=255)
                                                                  aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                  >  1:692286/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 <  1:3654156/93‑1 (MQ=255)
                                                                    tGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                >  2:2901360/1‑93 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               >  2:136/1‑93 (MQ=255)
                                                                      gTACAACGTCCTTTCATGCATTTCATGCCGAACAG‑‑‑GTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           <  1:4288636/93‑1 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             <  1:4183689/93‑1 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            >  2:6292694/1‑93 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            >  2:1849320/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           >  1:2970154/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           <  2:1035311/93‑1 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg          <  1:2554919/93‑1 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg          <  2:62314/93‑1 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca       <  1:3164969/93‑1 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTCCCGCACCCATCGTCGccc       >  1:4482142/1‑92 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccac      >  1:4702400/1‑93 (MQ=255)
                                                                                cTTTCATGTATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    >  2:6137870/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCATCCATCGTCGccacca    >  1:6189346/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    >  1:6113976/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    >  1:1301092/1‑93 (MQ=255)
                                                                                       ||                                                                                      
GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302533.6139950/94‑1 (MQ=60)
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302533.3214741/94‑1 (MQ=60)
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           >  SRR4302533.2040343/1‑94 (MQ=60)
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302533.1661620/94‑1 (MQ=60)
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTAACCTATCACTTTACTTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           >  SRR4302533.3119782/1‑94 (MQ=60)
     TGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTT                                                                                      >  SRR4302533.2138870/1‑94 (MQ=60)
      GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTC                                                                                     <  SRR4302533.711750/94‑1 (MQ=60)
       ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    <  SRR4302533.3303513/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTTATGCATTTCAT                                                                                   >  SRR4302533.1580520/1‑94 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302533.3037452/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   >  SRR4302533.4298159/1‑94 (MQ=60)
           AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                <  SRR4302533.927085/94‑1 (MQ=60)
           AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                >  SRR4302533.2752801/1‑94 (MQ=60)
           AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                <  SRR4302533.1413667/94‑1 (MQ=60)
            GGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCG                                                                               >  SRR4302533.3419603/1‑94 (MQ=60)
             GCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGA                                                                              <  SRR4302533.5821089/94‑1 (MQ=60)
             GCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGA                                                                              <  SRR4302533.6104108/94‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             >  SRR4302533.4801321/1‑94 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             >  SRR4302533.5904174/1‑94 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302533.6227062/94‑1 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302533.6168492/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGTCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          >  SRR4302533.2676006/1‑94 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302533.5572534/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          >  SRR4302533.5327742/1‑94 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302533.5388296/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          >  SRR4302533.4887312/1‑94 (MQ=60)
                  CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302533.1034091/1‑94 (MQ=60)
                  CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302533.6223197/1‑94 (MQ=60)
                  CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302533.384440/1‑94 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        >  SRR4302533.1336992/1‑94 (MQ=60)
                    CCGATTCTTTGACAGAACTAAACTGTTAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       >  SRR4302533.1901729/1‑94 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302533.4526742/1‑94 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302533.5807861/1‑94 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGGACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302533.4413199/1‑94 (MQ=60)
                      GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302533.3719220/94‑1 (MQ=60)
                      GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302533.5469310/94‑1 (MQ=60)
                       ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    <  SRR4302533.1250731/94‑1 (MQ=60)
                       ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302533.1120136/1‑94 (MQ=60)
                        TTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTG                                                                   >  SRR4302533.2179441/1‑94 (MQ=60)
                         TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  >  SRR4302533.1603266/1‑94 (MQ=60)
                         TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  <  SRR4302533.367521/94‑1 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302533.2144924/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302533.5471365/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 <  SRR4302533.607837/94‑1 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 <  SRR4302533.607838/94‑1 (MQ=60)
                            TTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGT                                                               <  SRR4302533.1719533/94‑1 (MQ=60)
                            TTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGT                                                               <  SRR4302533.1286993/94‑1 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              >  SRR4302533.4019874/1‑94 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302533.2187347/94‑1 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302533.6179166/94‑1 (MQ=60)
                              TACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302533.4879436/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302533.5787878/94‑1 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            <  SRR4302533.3824660/94‑1 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            <  SRR4302533.1179152/94‑1 (MQ=60)
                                CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           <  SRR4302533.5044093/94‑1 (MQ=60)
                                 AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302533.4833580/1‑94 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         >  SRR4302533.2345516/1‑94 (MQ=60)
                                   AACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGC                                                        >  SRR4302533.2351894/1‑94 (MQ=60)
                                    ACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCC                                                       <  SRR4302533.1668465/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302533.5862084/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302533.6052917/94‑1 (MQ=60)
                                     CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCCTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302533.3406245/94‑1 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302533.3087993/94‑1 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302533.2948850/94‑1 (MQ=60)
                                         ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  >  SRR4302533.3017819/1‑94 (MQ=60)
                                         ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  <  SRR4302533.4272926/94‑1 (MQ=60)
                                          CTGTGAGTTCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTC                                                 <  SRR4302533.3852616/94‑1 (MQ=60)
                                          CTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTC                                                 >  SRR4302533.3520119/1‑94 (MQ=60)
                                          CTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTC                                                 <  SRR4302533.1370175/94‑1 (MQ=60)
                                           TGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCG                                                <  SRR4302533.590952/94‑1 (MQ=60)
                                            GTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               >  SRR4302533.3654373/1‑94 (MQ=60)
                                            GTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               >  SRR4302533.3529291/1‑94 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302533.5732626/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              >  SRR4302533.2488574/1‑94 (MQ=60)
                                              GAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACG                                             >  SRR4302533.369890/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           >  SRR4302533.5139269/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           >  SRR4302533.4736065/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           >  SRR4302533.5141117/1‑94 (MQ=60)
                                                 TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          <  SRR4302533.4225782/94‑1 (MQ=60)
                                                 TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302533.5269287/1‑94 (MQ=60)
                                                  ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302533.127530/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302533.3601371/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302533.2284/1‑94 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302533.1087076/1‑94 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302533.910155/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      >  SRR4302533.5015691/1‑94 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  <  SRR4302533.6136670/94‑1 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  <  SRR4302533.5650081/94‑1 (MQ=60)
                                                          ACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGC                                 >  SRR4302533.6080982/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302533.4187950/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302533.368049/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302533.3862184/94‑1 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302533.3789572/1‑94 (MQ=60)
                                                            TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               >  SRR4302533.3151348/1‑94 (MQ=60)
                                                            TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               >  SRR4302533.252202/1‑94 (MQ=60)
                                                              TACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTC                             >  SRR4302533.4820582/1‑94 (MQ=60)
                                                              TACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCAAGCTTCGACGTTCCCCAACCGCTTTC                             <  SRR4302533.1543896/94‑1 (MQ=60)
                                                               ACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCT                            >  SRR4302533.3933391/1‑94 (MQ=60)
                                                                 CTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGC                          >  SRR4302533.6197518/1‑94 (MQ=60)
                                                                  TGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCT                         <  SRR4302533.2359969/94‑1 (MQ=60)
                                                                   GGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTG                        <  SRR4302533.2845807/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      <  SRR4302533.99004/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      >  SRR4302533.1182436/1‑94 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     >  SRR4302533.4126296/1‑94 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     >  SRR4302533.1494392/1‑94 (MQ=60)
                                                                       CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGC                    >  SRR4302533.696375/1‑94 (MQ=60)
                                                                       CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGC                    >  SRR4302533.2367230/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302533.4966870/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302533.439610/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   <  SRR4302533.4901321/94‑1 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   <  SRR4302533.3910663/94‑1 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302533.3290242/1‑94 (MQ=60)
                                                                         TTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCA                  <  SRR4302533.3676622/94‑1 (MQ=60)
                                                                         TTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCA                  >  SRR4302533.3021834/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 >  SRR4302533.3938865/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 <  SRR4302533.5795442/94‑1 (MQ=60)
                                                                            GTACAACGTCCTTTCATGCATTTCATGCCGAACAG‑‑‑GTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            <  SRR4302533.4314426/94‑1 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              <  SRR4302533.4209024/94‑1 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGC              >  SRR4302533.3761631/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302533.2988313/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302533.2660272/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302533.356981/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302533.4296008/94‑1 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302533.3973668/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302533.1373955/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302533.2629072/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302533.1821930/1‑94 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGC           <  SRR4302533.2570636/94‑1 (MQ=60)
                                                                                 ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          >  SRR4302533.6263421/1‑94 (MQ=60)
                                                                                  CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCA         >  SRR4302533.2304581/1‑94 (MQ=60)
                                                                                  CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTCCCGCACCCATCGTCGCCC         >  SRR4302533.4509284/1‑94 (MQ=60)
                                                                                   GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        <  SRR4302533.3184446/94‑1 (MQ=60)
                                                                                   GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302533.4730909/1‑94 (MQ=60)
                                                                                   GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302533.1321430/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCATCCATCGTCGCCACCA      >  SRR4302533.6226488/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302533.6150870/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302533.295724/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302533.1308918/1‑94 (MQ=60)
                                                                                        TTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   >  SRR4302533.5688918/1‑94 (MQ=60)
                                                                                        TTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   <  SRR4302533.4774472/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302533.2311186/1‑94 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302533.2465527/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302533.1658952/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302533.1542092/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302533.5929098/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302533.3300002/1‑94 (MQ=60)
                                                                                         TCATGCATTTCATGCAGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302533.260821/94‑1 (MQ=60)
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑