Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,740,514 T→C R51R (CGT→CGC)  yiaK → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,740,5140TC99.8% 1763.9 / ‑5.6 540R51R (CGT→CGC) yiaKputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (215/324);  total (215/325)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 552 total aligned reads displayed.

TGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGC  >  NC_000913/3740424‑3740603
                                                                                          |                                                                                         
    aGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCttcatt                                                                                     <  1:8409193/93‑1 (MQ=255)
     gCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCttcattc                                                                                    >  1:1118022/1‑93 (MQ=255)
      cGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCttcattca                                                                                   <  1:9859278/93‑1 (MQ=255)
       gAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTcaa                                                                                  >  2:7064144/1‑93 (MQ=255)
         aaCGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTcaaca                                                                                >  1:8456323/1‑93 (MQ=255)
          aCGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTcaacaa                                                                               <  2:9705572/93‑1 (MQ=255)
          aCGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTcaacaa                                                                               >  2:6736744/1‑93 (MQ=255)
           cGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCaacaac                                                                              <  2:4953232/93‑1 (MQ=255)
            ggCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACt                                                                             <  2:3673776/93‑1 (MQ=255)
            ggCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACt                                                                             >  1:7715725/1‑93 (MQ=255)
             gCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTg                                                                            <  2:2354809/93‑1 (MQ=255)
             gCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTg                                                                            >  2:6535315/1‑93 (MQ=255)
              cTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTgg                                                                           <  1:7869907/93‑1 (MQ=255)
              cTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTgg                                                                           <  1:6220804/93‑1 (MQ=255)
              cTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTgg                                                                           >  1:3882664/1‑93 (MQ=255)
                gACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGaa                                                                         <  2:4609717/93‑1 (MQ=255)
                   gCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAAc                                                                      <  1:3985265/93‑1 (MQ=255)
                      tgtgCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGc                                                                   <  2:4146469/93‑1 (MQ=255)
                      tgtgCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGc                                                                   >  1:5174009/1‑93 (MQ=255)
                        tgCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGa                                                                 <  2:4266635/93‑1 (MQ=255)
                        tgCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGa                                                                 <  2:8195319/93‑1 (MQ=255)
                         gCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGat                                                                >  1:2194590/1‑93 (MQ=255)
                         gCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGat                                                                <  2:7643505/93‑1 (MQ=255)
                         gCAGAGATGTTCGCCCGCACCACCGAACCCGGCGTTTATTCACACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGat                                                                <  2:1969301/93‑1 (MQ=255)
                          cAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGata                                                               >  1:7419142/1‑93 (MQ=255)
                           agagATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGatat                                                              <  2:2760793/93‑1 (MQ=255)
                            gagaTGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATc                                                             <  1:5056745/93‑1 (MQ=255)
                            gagaTGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATc                                                             <  2:3086911/93‑1 (MQ=255)
                              gaTGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCAt                                                           <  2:5034080/93‑1 (MQ=255)
                                tGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTc                                                         <  2:4803929/93‑1 (MQ=255)
                                tGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTc                                                         <  1:8624753/93‑1 (MQ=255)
                                tGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTc                                                         <  1:6931790/93‑1 (MQ=255)
                                  ttCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCt                                                       >  1:5920555/1‑93 (MQ=255)
                                   tCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTg                                                      >  2:9192978/1‑93 (MQ=255)
                                    cGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGa                                                     <  2:5371888/93‑1 (MQ=255)
                                    cGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGa                                                     <  1:4324526/93‑1 (MQ=255)
                                      cccGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATg                                                   >  2:5213622/1‑93 (MQ=255)
                                        cGCACCACCGAATCCGGCGTTTATTCACACGGCGTTAATCGGTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcc                                                 >  2:1099477/1‑93 (MQ=255)
                                         gCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTTCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGccc                                                <  2:3469940/93‑1 (MQ=255)
                                         gCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATTccc                                                >  1:5650661/1‑93 (MQ=255)
                                         gCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGccc                                                >  2:5821014/1‑93 (MQ=255)
                                          caccacCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGccca                                               <  2:2078237/93‑1 (MQ=255)
                                            ccaccGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaac                                             >  1:2350159/1‑93 (MQ=255)
                                            ccaccGAATCCGGCGGTTATTCTCACGGCGTTACTCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaac                                             <  2:6340998/93‑1 (MQ=255)
                                             caccGAATCCGGCGTTTATTCTCGCGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaacc                                            <  1:8084554/93‑1 (MQ=255)
                                             caccGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaacc                                            <  2:1410764/93‑1 (MQ=255)
                                               ccGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaaccca                                          >  1:8304733/1‑93 (MQ=255)
                                                cGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGcccaacccaa                                         <  2:5619924/93‑1 (MQ=255)
                                                 gAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCaaa                                        <  2:354936/93‑1 (MQ=255)
                                                 gAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCaaa                                        <  2:3335679/93‑1 (MQ=255)
                                                  aaTCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAAc                                       >  2:1186429/1‑93 (MQ=255)
                                                    tCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGt                                     >  1:445981/1‑93 (MQ=255)
                                                    tCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGt                                     >  2:7421439/1‑93 (MQ=255)
                                                     ccGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGta                                    <  1:6000247/93‑1 (MQ=255)
                                                      cGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGtat                                   >  2:4043437/1‑93 (MQ=255)
                                                      cGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGtat                                   >  2:3817403/1‑93 (MQ=255)
                                                      cGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGtat                                   <  1:7142476/93‑1 (MQ=255)
                                                       ggCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGtata                                  >  1:1317611/1‑93 (MQ=255)
                                                        gCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATaa                                 <  2:950236/93‑1 (MQ=255)
                                                        gCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATaa                                 <  2:9442632/93‑1 (MQ=255)
                                                         cGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAc                                >  2:1684157/1‑93 (MQ=255)
                                                          gTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAcc                               <  1:4923325/93‑1 (MQ=255)
                                                          gTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAcc                               >  1:4712200/1‑93 (MQ=255)
                                                           tttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCa                              >  1:3700183/1‑93 (MQ=255)
                                                           tttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCa                              >  1:5421245/1‑93 (MQ=255)
                                                           tttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATAATTCCTGATGCCCAACCCAAACGTATAACCa                              >  2:266560/1‑93 (MQ=255)
                                                            ttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAg                             >  2:1880074/1‑93 (MQ=255)
                                                            ttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAg                             <  2:7952202/93‑1 (MQ=255)
                                                            ttATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAg                             >  2:6150644/1‑93 (MQ=255)
                                                             tATTCTCACGTCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGc                            <  2:8690118/93‑1 (MQ=255)
                                                              aTTCTCACGGCGTTAATCGTTTCCCTCGCTTCCTTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGcc                           >  1:4539309/1‑93 (MQ=255)
                                                              aTTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGcc                           >  2:9944137/1‑93 (MQ=255)
                                                              aTTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGcc                           >  1:9402155/1‑93 (MQ=255)
                                                               ttCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCt                          >  1:1703599/1‑93 (MQ=255)
                                                                tctcACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCACAACCCAAACGTATAACCAGCCTc                         <  2:7239570/93‑1 (MQ=255)
                                                                 ctcACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCg                        >  2:7809514/1‑93 (MQ=255)
                                                                 ctcACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCg                        <  1:5277687/93‑1 (MQ=255)
                                                                  tcACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCgg                       >  1:1928146/1‑93 (MQ=255)
                                                                  tcACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCgg                       <  1:6779774/93‑1 (MQ=255)
                                                                   cACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGgc                      <  2:9088512/93‑1 (MQ=255)
                                                                   cACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGgc                      <  1:2538937/93‑1 (MQ=255)
                                                                   cACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGgc                      <  1:3135993/93‑1 (MQ=255)
                                                                       gCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCaa                  <  1:6524057/93‑1 (MQ=255)
                                                                        cGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAATCGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAAt                 <  1:7569481/93‑1 (MQ=255)
                                                                        cGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAAt                 <  2:6919336/93‑1 (MQ=255)
                                                                          ttAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTg               >  1:8824232/1‑93 (MQ=255)
                                                                            taTCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGaa             <  1:3420860/92‑1 (MQ=255)
                                                                            aaTCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGaa             >  1:7289256/1‑93 (MQ=255)
                                                                             aTCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAc            >  1:9106823/1‑93 (MQ=255)
                                                                             aTCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAc            <  1:2094992/93‑1 (MQ=255)
                                                                             aTCGTTTCACTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAc            <  1:1427395/93‑1 (MQ=255)
                                                                               cGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAg          <  1:4179914/93‑1 (MQ=255)
                                                                               cGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAg          <  1:9551552/93‑1 (MQ=255)
                                                                                 tttCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTg        >  2:589430/1‑93 (MQ=255)
                                                                                    cccTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGa     <  2:4518465/93‑1 (MQ=255)
                                                                                    cccTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGa     <  1:105301/93‑1 (MQ=255)
                                                                                    cccTCGCTTCATGCAACAACTGGAAAACTGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGa     <  1:27948/93‑1 (MQ=255)
                                                                                     ccTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAc    >  1:4852087/1‑93 (MQ=255)
                                                                                     ccTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAc    <  1:6411728/93‑1 (MQ=255)
                                                                                       tCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGc  <  1:9715608/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGC  >  NC_000913/3740424‑3740603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 306 total aligned reads displayed.
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  NC_000913/3740422‑3740607
                                                                                            |                                                                                             
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTGTTCTTACGGCGCTAATATTTTCTCTCGTT                                                                                              >  SRR4302540.7881707/1‑94 (MQ=60)
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCT                                                                                              >  SRR4302540.9416418/1‑94 (MQ=60)
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCT                                                                                              >  SRR4302540.6684630/1‑94 (MQ=60)
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCT                                                                                              >  SRR4302540.6684629/1‑94 (MQ=60)
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCT                                                                                              <  SRR4302540.6486419/94‑1 (MQ=60)
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCT                                                                                              >  SRR4302540.5874238/1‑94 (MQ=60)
   GACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCA                                                                                           >  SRR4302540.9584790/1‑94 (MQ=60)
   GACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCA                                                                                           >  SRR4302540.4976210/1‑94 (MQ=60)
    GCAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCAT                                                                                          <  SRR4302540.1106742/94‑1 (MQ=60)
    ACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCAT                                                                                          <  SRR4302540.2130359/94‑1 (MQ=60)
     CAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATT                                                                                         <  SRR4302540.8473125/94‑1 (MQ=60)
     CAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCCCGCTTCATT                                                                                         >  SRR4302540.2106449/1‑94 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        >  SRR4302540.2036043/1‑94 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        <  SRR4302540.4390944/94‑1 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        <  SRR4302540.5717697/94‑1 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        <  SRR4302540.8506795/94‑1 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        >  SRR4302540.1129616/1‑94 (MQ=60)
      AGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTC                                                                                        >  SRR4302540.2029061/1‑94 (MQ=60)
       GCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCA                                                                                       <  SRR4302540.1901067/94‑1 (MQ=60)
        CGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAA                                                                                      <  SRR4302540.650652/94‑1 (MQ=60)
        CGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAA                                                                                      <  SRR4302540.9974480/94‑1 (MQ=60)
         GAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCCTTCAAA                                                                                     >  SRR4302540.5514762/1‑94 (MQ=60)
          AAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACA                                                                                    >  SRR4302540.8554512/1‑94 (MQ=60)
          AAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACA                                                                                    >  SRR4302540.10044723/1‑94 (MQ=60)
            ACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAAC                                                                                  <  SRR4302540.5557318/94‑1 (MQ=60)
            ACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAAC                                                                                  <  SRR4302540.6604104/94‑1 (MQ=60)
            ACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAAC                                                                                  <  SRR4302540.9402631/94‑1 (MQ=60)
             CGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACT                                                                                 <  SRR4302540.2597115/94‑1 (MQ=60)
             CGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACT                                                                                 <  SRR4302540.5987127/94‑1 (MQ=60)
              GGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTG                                                                                >  SRR4302540.448325/1‑94 (MQ=60)
              GGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTG                                                                                <  SRR4302540.219027/94‑1 (MQ=60)
              GGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATGCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTG                                                                                <  SRR4302540.9807358/94‑1 (MQ=60)
               GCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGG                                                                               >  SRR4302540.3924848/1‑94 (MQ=60)
               GCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGG                                                                               >  SRR4302540.4145577/1‑94 (MQ=60)
                CTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGA                                                                              >  SRR4302540.4570599/1‑94 (MQ=60)
                 TGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAA                                                                             <  SRR4302540.8521742/94‑1 (MQ=60)
                  GACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAA                                                                            <  SRR4302540.3379442/94‑1 (MQ=60)
                  GACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAA                                                                            >  SRR4302540.4635696/1‑94 (MQ=60)
                  GACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAA                                                                            >  SRR4302540.7523417/1‑94 (MQ=60)
                  GACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAA                                                                            >  SRR4302540.4585923/1‑94 (MQ=60)
                   ACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAA                                                                           <  SRR4302540.8816660/94‑1 (MQ=60)
                    CGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAAC                                                                          <  SRR4302540.1628354/94‑1 (MQ=60)
                    CGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAAC                                                                          <  SRR4302540.2338927/94‑1 (MQ=60)
                     GCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACG                                                                         <  SRR4302540.4028178/94‑1 (MQ=60)
                     GCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGCAAAACG                                                                         <  SRR4302540.8525131/94‑1 (MQ=60)
                      CCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAACACGG                                                                        >  SRR4302540.9357032/1‑94 (MQ=60)
                      CCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGG                                                                        <  SRR4302540.4964816/94‑1 (MQ=60)
                      CCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGG                                                                        <  SRR4302540.3519924/94‑1 (MQ=60)
                       CTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGC                                                                       <  SRR4302540.6032181/94‑1 (MQ=60)
                       CTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGC                                                                       <  SRR4302540.7507686/94‑1 (MQ=60)
                       CTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGC                                                                       >  SRR4302540.5670708/1‑94 (MQ=60)
                       CTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGC                                                                       <  SRR4302540.6275446/94‑1 (MQ=60)
                       CTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGC                                                                       >  SRR4302540.5230323/1‑94 (MQ=60)
                        TGTGCAGAGATGTTCGCCCGCACCATCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCG                                                                      >  SRR4302540.6314356/1‑94 (MQ=60)
                        TGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCG                                                                      >  SRR4302540.4564188/1‑94 (MQ=60)
                         GTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGA                                                                     <  SRR4302540.6038931/94‑1 (MQ=60)
                          TGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGAT                                                                    <  SRR4302540.9015735/94‑1 (MQ=60)
                          TGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGAT                                                                    >  SRR4302540.2218292/1‑94 (MQ=60)
                          TGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGAT                                                                    <  SRR4302540.8845511/94‑1 (MQ=60)
                          TGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGAT                                                                    <  SRR4302540.7723189/94‑1 (MQ=60)
                          TGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGAT                                                                    <  SRR4302540.7723190/94‑1 (MQ=60)
                           GCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATA                                                                   <  SRR4302540.5045542/94‑1 (MQ=60)
                           GCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATA                                                                   <  SRR4302540.5045543/94‑1 (MQ=60)
                           GCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATA                                                                   >  SRR4302540.47158/1‑94 (MQ=60)
                           GCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATA                                                                   <  SRR4302540.4749976/94‑1 (MQ=60)
                            CAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATAT                                                                  <  SRR4302540.5158652/94‑1 (MQ=60)
                              GAGATGTTCGCCCGCACCACCGAATCCGGTGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCA                                                                <  SRR4302540.9725958/94‑1 (MQ=60)
                              GAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCA                                                                <  SRR4302540.5111414/94‑1 (MQ=60)
                              GAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCA                                                                >  SRR4302540.7832026/1‑94 (MQ=60)
                               AGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCAT                                                               <  SRR4302540.6142026/94‑1 (MQ=60)
                               AGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCAT                                                               >  SRR4302540.4042122/1‑94 (MQ=60)
                                GATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATT                                                              <  SRR4302540.9689030/94‑1 (MQ=60)
                                 ATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTC                                                             <  SRR4302540.2010719/94‑1 (MQ=60)
                                  TGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCC                                                            <  SRR4302540.8724939/94‑1 (MQ=60)
                                  TGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCC                                                            <  SRR4302540.8724940/94‑1 (MQ=60)
                                   GTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCT                                                           >  SRR4302540.5985964/1‑94 (MQ=60)
                                   GTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCT                                                           <  SRR4302540.7918024/94‑1 (MQ=60)
                                   GTTCGCCCCCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGGTGCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCT                                                           <  SRR4302540.8085348/94‑1 (MQ=60)
                                    TTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTG                                                          <  SRR4302540.5307889/94‑1 (MQ=60)
                                     TCCCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGA                                                         <  SRR4302540.7385189/94‑1 (MQ=60)
                                      tggCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.7477619/91‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.7158844/94‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.9589065/94‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.9589066/94‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.1874285/94‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.4118258/94‑1 (MQ=60)
                                      CGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGAT                                                        <  SRR4302540.2468554/94‑1 (MQ=60)
                                       GCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATG                                                       >  SRR4302540.6809758/1‑94 (MQ=60)
                                       GCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATG                                                       <  SRR4302540.3446466/94‑1 (MQ=60)
                                       GCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATG                                                       <  SRR4302540.1780571/94‑1 (MQ=60)
                                       GCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATG                                                       <  SRR4302540.8934786/94‑1 (MQ=60)
                                        CCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGC                                                      >  SRR4302540.369541/1‑94 (MQ=60)
                                         CCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCC                                                     >  SRR4302540.4338359/1‑94 (MQ=60)
                                         CCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCC                                                     <  SRR4302540.8094122/94‑1 (MQ=60)
                                           GCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCA                                                   >  SRR4302540.3983700/1‑94 (MQ=60)
                                            gaaccCCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAA                                                  <  SRR4302540.9899506/89‑1 (MQ=60)
                                            CACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAA                                                  <  SRR4302540.4449876/94‑1 (MQ=60)
                                            CACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAA                                                  <  SRR4302540.2704389/94‑1 (MQ=60)
                                             ACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAAC                                                 >  SRR4302540.7642230/1‑94 (MQ=60)
                                             ACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAAC                                                 >  SRR4302540.2568183/1‑94 (MQ=60)
                                              CCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACC                                                >  SRR4302540.1067365/1‑94 (MQ=60)
                                              CCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACC                                                <  SRR4302540.4783752/94‑1 (MQ=60)
                                               CACCGAATCCGGCGTTTATTCTCGCGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCC                                               <  SRR4302540.8178383/94‑1 (MQ=60)
                                               CACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCC                                               <  SRR4302540.8374458/94‑1 (MQ=60)
                                               CACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCC                                               >  SRR4302540.1183705/1‑94 (MQ=60)
                                                ACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCA                                              <  SRR4302540.4752952/94‑1 (MQ=60)
                                                ACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCA                                              >  SRR4302540.8401168/1‑94 (MQ=60)
                                                ACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCA                                              <  SRR4302540.3895741/94‑1 (MQ=60)
                                                 CCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAA                                             <  SRR4302540.1380872/94‑1 (MQ=60)
                                                  CGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAA                                            >  SRR4302540.1480069/1‑94 (MQ=60)
                                                   GAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAAC                                           <  SRR4302540.8686539/94‑1 (MQ=60)
                                                   GAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAAC                                           <  SRR4302540.6884280/94‑1 (MQ=60)
                                                    AATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACG                                          <  SRR4302540.6509785/94‑1 (MQ=60)
                                                    AATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACG                                          <  SRR4302540.2171894/94‑1 (MQ=60)
                                                    AATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACG                                          <  SRR4302540.9855829/94‑1 (MQ=60)
                                                    AATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACG                                          <  SRR4302540.6726011/94‑1 (MQ=60)
                                                     ATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGT                                         >  SRR4302540.3977179/1‑94 (MQ=60)
                                                     ATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGT                                         >  SRR4302540.450733/1‑94 (MQ=60)
                                                       CCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTAT                                       <  SRR4302540.6465125/94‑1 (MQ=60)
                                                       CCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTAT                                       <  SRR4302540.7159783/94‑1 (MQ=60)
                                                       CCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTAT                                       <  SRR4302540.6066749/94‑1 (MQ=60)
                                                        CGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATA                                      <  SRR4302540.7430145/94‑1 (MQ=60)
                                                        CGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATA                                      >  SRR4302540.1331323/1‑94 (MQ=60)
                                                        CGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATA                                      <  SRR4302540.7222050/94‑1 (MQ=60)
                                                         GGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAA                                     >  SRR4302540.2321650/1‑94 (MQ=60)
                                                          GCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAC                                    <  SRR4302540.143777/94‑1 (MQ=60)
                                                          GCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAC                                    <  SRR4302540.2258122/94‑1 (MQ=60)
                                                          GCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAAC                                    <  SRR4302540.375430/94‑1 (MQ=60)
                                                           CGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACC                                   >  SRR4302540.1223018/1‑94 (MQ=60)
                                                           CGTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCATGATGCCCAACCCAAACGTATAACC                                   <  SRR4302540.8333928/94‑1 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  >  SRR4302540.5480859/1‑94 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  <  SRR4302540.8168059/94‑1 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  <  SRR4302540.5293207/94‑1 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  >  SRR4302540.3740572/1‑94 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  <  SRR4302540.730734/94‑1 (MQ=60)
                                                            GTTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCA                                  >  SRR4302540.9196505/1‑94 (MQ=60)
                                                             TTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGTCCAACCCAAACGTATAACCAG                                 <  SRR4302540.9552106/94‑1 (MQ=60)
                                                             TTTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAG                                 <  SRR4302540.4780749/94‑1 (MQ=60)
                                                              TTATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGC                                >  SRR4302540.5262674/1‑94 (MQ=60)
                                                               TATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCC                               >  SRR4302540.2928573/1‑94 (MQ=60)
                                                                ATTCTCACGGCGTTAATCGTTTCCCTCTCTTCATTCAACAACTGGAAAACAGCGATATCATTCCTGATGCCCAACCCAAACGTATATCCAGCCT                              >  SRR4302540.5444639/1‑94 (MQ=60)
                                                                ATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCT                              <  SRR4302540.839666/94‑1 (MQ=60)
                                                                ATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCT                              >  SRR4302540.1722029/1‑94 (MQ=60)
                                                                ATTCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCT                              >  SRR4302540.5444638/1‑94 (MQ=60)
                                                                  TCTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCG                            <  SRR4302540.6966079/94‑1 (MQ=60)
                                                                   CTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGG                           <  SRR4302540.726906/94‑1 (MQ=60)
                                                                   CTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGG                           <  SRR4302540.5335318/94‑1 (MQ=60)
                                                                   CTCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGG                           <  SRR4302540.1289658/94‑1 (MQ=60)
                                                                    TCACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGC                          <  SRR4302540.6854730/94‑1 (MQ=60)
                                                                     CACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCG                         <  SRR4302540.2483972/94‑1 (MQ=60)
                                                                     CACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCG                         <  SRR4302540.2566314/94‑1 (MQ=60)
                                                                     CACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCG                         <  SRR4302540.3170200/94‑1 (MQ=60)
                                                                     CACGGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCG                         <  SRR4302540.5673455/94‑1 (MQ=60)
                                                                       CGGCGTTAATCGTTTCCCTCGCTTCATTGCACGACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCTCA                       <  SRR4302540.4851452/94‑1 (MQ=60)
                                                                        GGCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAA                      <  SRR4302540.4306799/94‑1 (MQ=60)
                                                                         GCGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAAT                     <  SRR4302540.6596517/94‑1 (MQ=60)
                                                                          CGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAATCGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATT                    <  SRR4302540.7654398/94‑1 (MQ=60)
                                                                          CGTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATT                    >  SRR4302540.5168836/1‑94 (MQ=60)
                                                                           GTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTG                   <  SRR4302540.7341248/94‑1 (MQ=60)
                                                                           GTTAATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTG                   >  SRR4302540.8455614/1‑94 (MQ=60)
                                                                              TATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAC                <  SRR4302540.3458065/94‑1 (MQ=60)
                                                                              AATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAC                >  SRR4302540.9216263/1‑94 (MQ=60)
                                                                               ATCGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACA               >  SRR4302540.9676252/1‑94 (MQ=60)
                                                                               ATCGTTTCACTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACA               <  SRR4302540.1442847/94‑1 (MQ=60)
                                                                                 CGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGT             <  SRR4302540.4225310/94‑1 (MQ=60)
                                                                                 CGTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGT             <  SRR4302540.9880486/94‑1 (MQ=60)
                                                                                  GTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTG            <  SRR4302540.4351032/94‑1 (MQ=60)
                                                                                  GTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTG            >  SRR4302540.1473680/1‑94 (MQ=60)
                                                                                  GTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTG            >  SRR4302540.3133424/1‑94 (MQ=60)
                                                                                  GTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTG            >  SRR4302540.8592239/1‑94 (MQ=60)
                                                                                  GTTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTG            >  SRR4302540.5098598/1‑94 (MQ=60)
                                                                                   TTTCGCTCGCTTCATTCAACAACTGGAACACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAAGAGTGG           >  SRR4302540.6274359/1‑94 (MQ=60)
                                                                                   TTTCCCTCGCTTCATTCAACAACTGGAAAACTGCGATATCATTCCTGATGCCCAACCCAAACATATAACCAGCCTCGGCGCAATTGAACAGTGG           >  SRR4302540.6909662/1‑94 (MQ=60)
                                                                                   TTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGG           >  SRR4302540.5921448/1‑94 (MQ=60)
                                                                                   TTTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGG           <  SRR4302540.3761530/94‑1 (MQ=60)
                                                                                    TTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGG          <  SRR4302540.7386865/94‑1 (MQ=60)
                                                                                    TTCCCTCGCTTCATTCAACACCTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATACCCAGCCTCGGCGCAATTGAACAGTGGG          <  SRR4302540.4966340/94‑1 (MQ=60)
                                                                                    TTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGG          <  SRR4302540.7027803/94‑1 (MQ=60)
                                                                                    TTCCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGG          >  SRR4302540.7766719/1‑94 (MQ=60)
                                                                                      CCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAC        <  SRR4302540.106424/94‑1 (MQ=60)
                                                                                      CCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAC        >  SRR4302540.4904086/1‑94 (MQ=60)
                                                                                      CCCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAC        >  SRR4302540.28965/1‑94 (MQ=60)
                                                                                      CCCTCGCTTCATGCAACAACTGGAAAACTGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGAC        <  SRR4302540.28247/94‑1 (MQ=60)
                                                                                       CCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACG       >  SRR4302540.8565304/1‑94 (MQ=60)
                                                                                       CCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACG       <  SRR4302540.6409009/94‑1 (MQ=60)
                                                                                       CCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACG       >  SRR4302540.3203382/1‑94 (MQ=60)
                                                                                       CCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACG       <  SRR4302540.6483081/94‑1 (MQ=60)
                                                                                       CCTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTACTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACG       >  SRR4302540.7903853/1‑94 (MQ=60)
                                                                                        CTCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGC      >  SRR4302540.5143995/1‑94 (MQ=60)
                                                                                         TCGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCC     >  SRR4302540.9128931/1‑94 (MQ=60)
                                                                                          CGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCC    >  SRR4302540.2395199/1‑94 (MQ=60)
                                                                                          CGCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCC    >  SRR4302540.9835010/1‑94 (MQ=60)
                                                                                           GCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACATTGGGACGCCCA   >  SRR4302540.5793516/1‑94 (MQ=60)
                                                                                           GCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCA   >  SRR4302540.8070658/1‑94 (MQ=60)
                                                                                           GCTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCA   <  SRR4302540.1221770/94‑1 (MQ=60)
                                                                                            CTTCATTCAACAACTGGAAAACGGCGATATTATTCCTGATGCCCAACCCAAACGTATAACTAGCCTCGGCGTAATTGAACAGTGGGACGCCCAG  >  SRR4302540.9085761/1‑94 (MQ=60)
                                                                                            CTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  SRR4302540.8099397/1‑94 (MQ=60)
                                                                                            CTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  SRR4302540.2358822/1‑94 (MQ=60)
                                                                                            CTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  SRR4302540.2832708/1‑94 (MQ=60)
                                                                                            CTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  SRR4302540.1493409/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTTGACAGCGAAACGGCTGACGCCTGTGCAGAGATGTTCGCCCGCACCACCGAATCCGGCGTTTATTCTCACGGCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAG  >  NC_000913/3740422‑3740607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑