Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,804,546	T→C	E50G (GAG→GGG)	rfaG ←	glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,804,546	0	T	C	99.8%	1768.1 / ‑5.3	535	E50G (GAG→GGG)	rfaG	glucosyltransferase I
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (245/287); total (246/289)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 545 total aligned reads displayed.

GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATA  >  NC_000913/3804456‑3804635

ggATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCa                                                                                         <  1:4359222/93‑1 (MQ=255)

ggATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTAAATAAGCCCa                                                                                         >  1:1830346/1‑93 (MQ=255)

 gATGCTCTTTGAGATGATTTTGTTCCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGTCACCTGAATAAGCCCaa                                                                                        <  2:7288555/93‑1 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       <  2:3953043/93‑1 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       <  2:1075393/93‑1 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       >  2:7784352/1‑93 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       <  2:8680676/93‑1 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       <  2:9184826/93‑1 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       >  1:4867443/1‑93 (MQ=255)

  aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCaaa                                                                                       >  1:3188692/1‑93 (MQ=255)

   tGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAt                                                                                      >  1:6413070/1‑93 (MQ=255)

    gCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATg                                                                                     <  2:6585154/93‑1 (MQ=255)

      tctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCt                                                                                   >  1:1497198/1‑93 (MQ=255)

       ctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCtt                                                                                  >  1:1000796/1‑93 (MQ=255)

       ctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCtt                                                                                  <  2:8870752/93‑1 (MQ=255)

        tttGATATGATGTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  1:7843701/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGGCTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:4664400/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  1:7774575/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  1:3013848/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 >  1:7008374/1‑93 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:2542102/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:3604488/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:3276051/93‑1 (MQ=255)

          tGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCg                                                                               <  2:9323383/93‑1 (MQ=255)

           gagaTGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCgg                                                                              <  1:1283387/93‑1 (MQ=255)

            agaTGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGATATGGCACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCggg                                                                             <  1:1750251/93‑1 (MQ=255)

              atgatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCa                                                                           <  2:5196824/93‑1 (MQ=255)

                gatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAt                                                                         <  1:3997484/93‑1 (MQ=255)

                gatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAt                                                                         <  2:5963504/93‑1 (MQ=255)

                 atTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATc                                                                        >  1:9084674/1‑93 (MQ=255)

                  ttttGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCg                                                                       >  2:6365976/1‑93 (MQ=255)

                    ttGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGcc                                                                     >  1:1397685/1‑93 (MQ=255)

                      gTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCtt                                                                   <  2:6031354/93‑1 (MQ=255)

                      gTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCtt                                                                   <  2:4537219/93‑1 (MQ=255)

                       tACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTc                                                                  <  1:163310/93‑1 (MQ=255)

                            aGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACg                                                             >  2:7887093/1‑93 (MQ=255)

                             ggCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGa                                                            <  2:7617134/93‑1 (MQ=255)

                             ggCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGa                                                            <  1:231426/93‑1 (MQ=255)

                             ggCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGa                                                            >  2:8532601/1‑93 (MQ=255)

                             ggCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGa                                                            >  1:7517914/1‑93 (MQ=255)

                              gCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCGCCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGAc                                                           <  2:5031262/93‑1 (MQ=255)

                              gCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGAc                                                           <  2:82786/93‑1 (MQ=255)

                              gCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGAc                                                           <  1:8634699/93‑1 (MQ=255)

                                ataataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCGAATGCTTTCGGGCAATCGCCTTCCCACGACtg                                                         >  2:6953859/1‑93 (MQ=255)

                                ataataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACtg                                                         <  2:1699180/93‑1 (MQ=255)

                                 taataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACtgt                                                        >  2:7506831/1‑93 (MQ=255)

                                   atatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTgtgt                                                      >  2:9635061/1‑93 (MQ=255)

                                   atatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTgtgt                                                      <  1:528434/93‑1 (MQ=255)

                                   atatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTgtgt                                                      >  2:4331541/1‑93 (MQ=255)

                                      ttCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtata                                                   <  2:538546/93‑1 (MQ=255)

                                      ttCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtata                                                   <  1:2664196/93‑1 (MQ=255)

                                       tCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTatatat                                                  >  1:9233805/1‑93 (MQ=255)

                                        cTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                 >  1:3650297/1‑93 (MQ=255)

                                        cTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                 <  1:5593593/93‑1 (MQ=255)

                                        cTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                 <  2:2400232/93‑1 (MQ=255)

                                         tGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAc                                                <  1:8182716/93‑1 (MQ=255)

                                          gCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAcc                                               >  1:6502903/1‑93 (MQ=255)

                                           cATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAccc                                              >  1:6820232/1‑93 (MQ=255)

                                            aTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCg                                             >  2:3472905/1‑93 (MQ=255)

                                             ttGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTTCCACGACTGTGTATATACCCGa                                            >  1:7736088/1‑93 (MQ=255)

                                               gCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAc                                          <  2:3002126/93‑1 (MQ=255)

                                                cGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACa                                         >  2:1341360/1‑93 (MQ=255)

                                                 gTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAt                                        >  2:4129768/1‑93 (MQ=255)

                                                 gTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAt                                        <  1:7125733/93‑1 (MQ=255)

                                                 gTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAt                                        <  2:5715331/93‑1 (MQ=255)

                                                 gTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGCGTATATACCCGAACAt                                        <  2:9556923/93‑1 (MQ=255)

                                                  tCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtg                                       >  2:3895481/1‑93 (MQ=255)

                                                   ccATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtgg                                      >  1:2568539/1‑93 (MQ=255)

                                                   ccATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtgg                                      >  1:9530866/1‑93 (MQ=255)

                                                     aTGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtggtg                                    >  1:579091/1‑93 (MQ=255)

                                                     aTGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtggtg                                    <  2:513599/93‑1 (MQ=255)

                                                      tggttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtggtgg                                   >  2:9812063/1‑93 (MQ=255)

                                                      tggttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtggtgg                                   <  1:10347/93‑1 (MQ=255)

                                                         ttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGccc                                <  1:8415252/93‑1 (MQ=255)

                                                         ttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGccc                                >  1:9930876/1‑93 (MQ=255)

                                                           ggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCg                              >  1:9706944/1‑93 (MQ=255)

                                                           ggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCg                              <  1:2834796/93‑1 (MQ=255)

                                                            gtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGt                             >  2:2754440/1‑93 (MQ=255)

                                                             tATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTg                            >  1:2022025/1‑93 (MQ=255)

                                                              aTGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGc                           <  1:8891864/93‑1 (MQ=255)

                                                              aTGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGc                           <  1:7360860/93‑1 (MQ=255)

                                                              aTGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGc                           <  1:5112358/93‑1 (MQ=255)

                                                               tGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCg                          >  2:7954364/1‑93 (MQ=255)

                                                               tGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCg                          <  1:8030504/93‑1 (MQ=255)

                                                                     ttAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACt                    >  2:9446067/1‑93 (MQ=255)

                                                                       aaCTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGt                  <  1:9349144/93‑1 (MQ=255)

                                                                         cTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTg                <  2:44751/93‑1 (MQ=255)

                                                                          tGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGa               <  2:2122316/93‑1 (MQ=255)

                                                                          tGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGa               <  2:7037376/93‑1 (MQ=255)

                                                                          tGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGa               <  2:4812601/93‑1 (MQ=255)

                                                                            gCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATg             <  2:4896100/93‑1 (MQ=255)

                                                                            gCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATg             <  1:4608561/93‑1 (MQ=255)

                                                                            gCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATg             <  2:1916277/93‑1 (MQ=255)

                                                                              aCCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCa           <  1:5904376/93‑1 (MQ=255)

                                                                              aCCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCa           >  2:6211057/1‑93 (MQ=255)

                                                                                cTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt         <  2:8117069/93‑1 (MQ=255)

                                                                                cTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt         <  2:9870488/93‑1 (MQ=255)

                                                                                   aaTAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATgcg      >  1:6074252/1‑93 (MQ=255)

                                                                                    aTAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCCATgcgc     >  2:5503299/1‑93 (MQ=255)

                                                                                     tAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCa    <  1:335177/93‑1 (MQ=255)

GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATA  >  NC_000913/3804456‑3804635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 276 total aligned reads displayed.

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  NC_000913/3804453‑3804639

 CGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCC                                                                                              >  SRR4302540.2807954/1‑94 (MQ=60)

  GGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCA                                                                                             <  SRR4302540.4824128/94‑1 (MQ=60)

  GGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTAAATAAGCCCA                                                                                             >  SRR4302540.1850372/1‑94 (MQ=60)

   GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAA                                                                                            <  SRR4302540.4406631/94‑1 (MQ=60)

    GATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAA                                                                                           >  SRR4302540.3223329/1‑94 (MQ=60)

    GATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAA                                                                                           >  SRR4302540.4919642/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          <  SRR4302540.5960279/94‑1 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          >  SRR4302540.2836141/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          <  SRR4302540.7251308/94‑1 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          >  SRR4302540.1159201/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          >  SRR4302540.1792591/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          >  SRR4302540.1792589/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          >  SRR4302540.9324605/1‑94 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGACCAAAT                                                                                          >  SRR4302540.10032743/1‑94 (MQ=60)

      TGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATG                                                                                         >  SRR4302540.10059273/1‑94 (MQ=60)

        CTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCT                                                                                       >  SRR4302540.1513679/1‑94 (MQ=60)

         TCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTT                                                                                      <  SRR4302540.110003/94‑1 (MQ=60)

         TCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTT                                                                                      >  SRR4302540.1010959/1‑94 (MQ=60)

         TCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTT                                                                                      <  SRR4302540.7154657/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCCCCTGAATAACCCCAACTGCTTT                                                                                     >  SRR4302540.7086165/1‑94 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302540.7605991/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     >  SRR4302540.7086166/1‑94 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     >  SRR4302540.1444497/1‑94 (MQ=60)

           TTTGATATGATGTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTC                                                                                    <  SRR4302540.7935351/94‑1 (MQ=60)

           TTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTC                                                                                    <  SRR4302540.3046989/94‑1 (MQ=60)

            TTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCACATGCTTTCG                                                                                   >  SRR4302540.6931870/1‑94 (MQ=60)

            TTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCG                                                                                   <  SRR4302540.7918007/94‑1 (MQ=60)

            TTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCG                                                                                   >  SRR4302540.8682531/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  >  SRR4302540.1026773/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  <  SRR4302540.9696556/94‑1 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGCTGGTATGGGCCTTAACTGGCACCTGAATAAGCCCAAATGCTTcccg                                                                                  >  SRR4302540.3595091/1‑90 (MQ=60)

              GAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGG                                                                                 >  SRR4302540.7884212/1‑94 (MQ=60)

              GAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGG                                                                                 <  SRR4302540.1296616/94‑1 (MQ=60)

               AGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGC                                                                                <  SRR4302540.2745095/94‑1 (MQ=60)

               AGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGC                                                                                >  SRR4302540.7408214/1‑94 (MQ=60)

                GATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCA                                                                               >  SRR4302540.4024285/1‑94 (MQ=60)

                 ATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAA                                                                              <  SRR4302540.614792/94‑1 (MQ=60)

                  TGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAT                                                                             <  SRR4302540.4913102/94‑1 (MQ=60)

                   TATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATC                                                                            <  SRR4302540.8206168/94‑1 (MQ=60)

                   GATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATC                                                                            <  SRR4302540.4040509/94‑1 (MQ=60)

                   GATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATC                                                                            <  SRR4302540.3427014/94‑1 (MQ=60)

                    ATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCG                                                                           <  SRR4302540.4423397/94‑1 (MQ=60)

                     TTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGC                                                                          <  SRR4302540.46921/94‑1 (MQ=60)

                      TTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCC                                                                         >  SRR4302540.1412693/1‑94 (MQ=60)

                      TTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCC                                                                         >  SRR4302540.7799544/1‑94 (MQ=60)

                      TTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCC                                                                         <  SRR4302540.4535625/94‑1 (MQ=60)

                         GTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTC                                                                      <  SRR4302540.8526626/94‑1 (MQ=60)

                         GTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTC                                                                      >  SRR4302540.4252721/1‑94 (MQ=60)

                         GTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTC                                                                      <  SRR4302540.3730302/94‑1 (MQ=60)

                          TACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCC                                                                     >  SRR4302540.3082297/1‑94 (MQ=60)

                          TACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCC                                                                     <  SRR4302540.164946/94‑1 (MQ=60)

                          TACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCC                                                                     <  SRR4302540.164947/94‑1 (MQ=60)

                            CCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCA                                                                   >  SRR4302540.2072220/1‑94 (MQ=60)

                             CCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAC                                                                  <  SRR4302540.3247616/94‑1 (MQ=60)

                             CCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAC                                                                  >  SRR4302540.8733841/1‑94 (MQ=60)

                             CCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAC                                                                  >  SRR4302540.2720101/1‑94 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTACCTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 >  SRR4302540.6372245/1‑94 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302540.7969239/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302540.8059541/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302540.9608857/94‑1 (MQ=60)

                                GGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGAC                                                               <  SRR4302540.233662/94‑1 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGTACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              >  SRR4302540.1801771/1‑94 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              <  SRR4302540.8153101/94‑1 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              >  SRR4302540.6689914/1‑94 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              <  SRR4302540.8769671/94‑1 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              <  SRR4302540.8769672/94‑1 (MQ=60)

                                 GCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACT                                                              <  SRR4302540.5646110/94‑1 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302540.5598648/1‑94 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302540.9083301/1‑94 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            <  SRR4302540.1691038/94‑1 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302540.2005149/1‑94 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302540.9486824/1‑94 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGG                                                            >  SRR4302540.8163272/1‑94 (MQ=60)

                                    TAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTG                                                           >  SRR4302540.9211654/1‑94 (MQ=60)

                                    TAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTG                                                           <  SRR4302540.5145684/94‑1 (MQ=60)

                                    TAATACTCTGCTTTGCGTCCATGGTTGGTATGGGGCTTAACTTGCACCTGACTAAGCCCAAATCCTTTCGCGCAATCGCCTTCCCACGACTGTG                                                           <  SRR4302540.5145686/94‑1 (MQ=60)

                                     AATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGT                                                          >  SRR4302540.1409456/1‑94 (MQ=60)

                                     AATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGT                                                          >  SRR4302540.1409461/1‑94 (MQ=60)

                                      ATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTA                                                         <  SRR4302540.9384853/94‑1 (MQ=60)

                                      ATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTA                                                         <  SRR4302540.534148/94‑1 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGTACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        <  SRR4302540.6706364/94‑1 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        <  SRR4302540.7185079/94‑1 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        >  SRR4302540.1583082/1‑94 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCAGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        <  SRR4302540.2479577/94‑1 (MQ=60)

                                        ATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATA                                                       >  SRR4302540.10105202/1‑94 (MQ=60)

                                        ATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATA                                                       >  SRR4302540.7894222/1‑94 (MQ=60)

                                        ATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGGATA                                                       >  SRR4302540.7894223/1‑94 (MQ=60)

                                         TTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATAT                                                      <  SRR4302540.7832203/94‑1 (MQ=60)

                                         TTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATAT                                                      <  SRR4302540.9767847/94‑1 (MQ=60)

                                         TTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATAT                                                      <  SRR4302540.3836481/94‑1 (MQ=60)

                                         TTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTATATAT                                                      >  SRR4302540.9344459/1‑94 (MQ=60)

                                         TTCTGCAGTTCGTCCATGGTTGGTAGGGAACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATAT                                                      <  SRR4302540.4755677/94‑1 (MQ=60)

                                           CTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAC                                                    <  SRR4302540.5655138/94‑1 (MQ=60)

                                           CTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAC                                                    >  SRR4302540.248109/1‑94 (MQ=60)

                                            TGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACC                                                   >  SRR4302540.6575189/1‑94 (MQ=60)

                                            TGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACC                                                   <  SRR4302540.8277760/94‑1 (MQ=60)

                                            TGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACC                                                   >  SRR4302540.595967/1‑94 (MQ=60)

                                            TGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACC                                                   >  SRR4302540.5898888/1‑94 (MQ=60)

                                             GCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCC                                                  <  SRR4302540.9964931/94‑1 (MQ=60)

                                             GCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCC                                                  >  SRR4302540.5994029/1‑94 (MQ=60)

                                             GCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCC                                                  >  SRR4302540.9206127/1‑94 (MQ=60)

                                             GCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCC                                                  <  SRR4302540.6508533/94‑1 (MQ=60)

                                             GCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCC                                                  >  SRR4302540.6895730/1‑94 (MQ=60)

                                              CATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCG                                                 <  SRR4302540.7861469/94‑1 (MQ=60)

                                              CATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCG                                                 >  SRR4302540.8839016/1‑94 (MQ=60)

                                               CTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGA                                                <  SRR4302540.369165/94‑1 (MQ=60)

                                               ATTGCGTCCATGGTTGGTATGTGTCTTAACTCGCACCTGAATAAGCCCAAATGCCTTCGGGCAATCGACTTCCCACGACTGTGTATATACCCGA                                                >  SRR4302540.3673573/1‑94 (MQ=60)

                                               ATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTTCCACGACTGTGTATATACCCGA                                                >  SRR4302540.7826405/1‑94 (MQ=60)

                                               ATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGA                                                >  SRR4302540.7224314/1‑94 (MQ=60)

                                               ATTGCGTCCATGGTTGGTACGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGA                                                >  SRR4302540.4957732/1‑94 (MQ=60)

                                               ATTGCGTCCATGGCTGGTATGGGACTTAACTGGCACCTGAATAAGCACAAATGCTTTCGGGCAATCGCCTTACCACGACTGTGTATATACCCGA                                                >  SRR4302540.3068499/1‑94 (MQ=60)

                                                TTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAA                                               >  SRR4302540.8997588/1‑94 (MQ=60)

                                                TTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATAGCCTTCCCACGACTGTGTATATACCCGAA                                               >  SRR4302540.5063880/1‑94 (MQ=60)

                                                 TGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAC                                              >  SRR4302540.106257/1‑94 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.7862580/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.2620157/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.6390615/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.4640688/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.6049742/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302540.1622956/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            >  SRR4302540.9202835/1‑94 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACCTG                                           >  SRR4302540.3629347/1‑94 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATG                                           >  SRR4302540.4884294/1‑94 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATG                                           >  SRR4302540.6350399/1‑94 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATG                                           >  SRR4302540.8575917/1‑94 (MQ=60)

                                                     TCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGG                                          <  SRR4302540.7250372/94‑1 (MQ=60)

                                                     TCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGG                                          >  SRR4302540.657383/1‑94 (MQ=60)

                                                     TCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGG                                          >  SRR4302540.2596235/1‑94 (MQ=60)

                                                     TCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGG                                          >  SRR4302540.9643698/1‑94 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         <  SRR4302540.1497997/94‑1 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         <  SRR4302540.7484922/94‑1 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         >  SRR4302540.1387102/1‑94 (MQ=60)

                                                      CCATGGGTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         <  SRR4302540.3212695/94‑1 (MQ=60)

                                                       CATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTG                                        <  SRR4302540.208333/94‑1 (MQ=60)

                                                       CATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTG                                        <  SRR4302540.208331/94‑1 (MQ=60)

                                                       CATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTG                                        <  SRR4302540.6912071/94‑1 (MQ=60)

                                                       CATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTG                                        >  SRR4302540.585361/1‑94 (MQ=60)

                                                        ATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGG                                       >  SRR4302540.7701347/1‑94 (MQ=60)

                                                        ATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGG                                       <  SRR4302540.5463179/94‑1 (MQ=60)

                                                         TGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGC                                      <  SRR4302540.10458/94‑1 (MQ=60)

                                                         TGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGC                                      >  SRR4302540.5888917/1‑94 (MQ=60)

                                                         TGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGC                                      <  SRR4302540.262274/94‑1 (MQ=60)

                                                          GGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCC                                     >  SRR4302540.10007554/1‑94 (MQ=60)

                                                          GGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCC                                     <  SRR4302540.952448/94‑1 (MQ=60)

                                                            TTGGTATGTGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302540.18562/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302540.8512926/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302540.29636/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTCTATATACCCGAACATGGTGGCCCC                                   <  SRR4302540.6569264/94‑1 (MQ=60)

                                                             TGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCG                                  >  SRR4302540.9821032/1‑94 (MQ=60)

                                                              GGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGT                                 >  SRR4302540.8253910/1‑94 (MQ=60)

                                                              GGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGT                                 >  SRR4302540.10020406/1‑94 (MQ=60)

                                                               GTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTG                                >  SRR4302540.6491182/1‑94 (MQ=60)

                                                               GTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTG                                <  SRR4302540.9487173/94‑1 (MQ=60)

                                                                TATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGC                               >  SRR4302540.5299961/1‑94 (MQ=60)

                                                                 ATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCG                              <  SRR4302540.8994796/94‑1 (MQ=60)

                                                                 ATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCG                              <  SRR4302540.5167698/94‑1 (MQ=60)

                                                                 ATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCG                              <  SRR4302540.7442940/94‑1 (MQ=60)

                                                                 ATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCG                              >  SRR4302540.2918837/1‑94 (MQ=60)

                                                                  TGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGG                             <  SRR4302540.7578189/94‑1 (MQ=60)

                                                                   GGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGC                            <  SRR4302540.1490338/94‑1 (MQ=60)

                                                                       CTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACT                        >  SRR4302540.9414033/1‑94 (MQ=60)

                                                                       CTTAACTAGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACT                        >  SRR4302540.7633664/1‑94 (MQ=60)

                                                                         TAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGT                      >  SRR4302540.4296018/1‑94 (MQ=60)

                                                                         TAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGT                      >  SRR4302540.9383127/1‑94 (MQ=60)

                                                                          AACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTT                     <  SRR4302540.9460504/94‑1 (MQ=60)

                                                                           ACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTG                    >  SRR4302540.4161271/1‑94 (MQ=60)

                                                                           ACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTG                    <  SRR4302540.5717966/94‑1 (MQ=60)

                                                                            CTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGA                   <  SRR4302540.10133160/94‑1 (MQ=60)

                                                                            CTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGA                   >  SRR4302540.2876457/1‑94 (MQ=60)

                                                                            CTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGA                   <  SRR4302540.3538599/94‑1 (MQ=60)

                                                                            CTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGA                   >  SRR4302540.1841566/1‑94 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.2689053/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.5734888/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.8423116/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.2834132/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.5496881/94‑1 (MQ=60)

                                                                             TGCCACGTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACGGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.8388807/94‑1 (MQ=60)

                                                                             CGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302540.7195249/94‑1 (MQ=60)

                                                                              GGCACCTGAATAAGCCCAAATTCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATG                 <  SRR4302540.1699273/94‑1 (MQ=60)

                                                                              GGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCAGTGCGGCAACTGTTGATG                 <  SRR4302540.5980122/94‑1 (MQ=60)

                                                                                 ACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGACCATGGTGGCCCCGTGCGGCAACTGTTGATGCAG              >  SRR4302540.3328144/1‑94 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            <  SRR4302540.7998803/94‑1 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            <  SRR4302540.3094291/94‑1 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            <  SRR4302540.1565122/94‑1 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            >  SRR4302540.2018421/1‑94 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGTGGCAACTGTTGATGCAATGCG          >  SRR4302540.5288203/1‑94 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCG          <  SRR4302540.336848/94‑1 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCG          >  SRR4302540.1126120/1‑94 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCG          >  SRR4302540.6141799/1‑94 (MQ=60)

                                                                                      AATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGC         >  SRR4302540.2326792/1‑94 (MQ=60)

                                                                                      AATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGC         >  SRR4302540.7530762/1‑94 (MQ=60)

                                                                                        TAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCAT       >  SRR4302540.9014572/1‑94 (MQ=60)

                                                                                           GCCCAAATGCTTTCGGGCAATCGCCTTTCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAA    >  SRR4302540.5876693/1‑94 (MQ=60)

                                                                                            CCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAG   <  SRR4302540.2669226/94‑1 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  <  SRR4302540.2313876/94‑1 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  <  SRR4302540.905979/94‑1 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  SRR4302540.2876016/1‑94 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  SRR4302540.4155708/1‑94 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGC  >  SRR4302540.2702422/1‑94 (MQ=60)

                                                                                             CCAAATGCTATCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGC  >  SRR4302540.8478081/1‑94 (MQ=60)

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  NC_000913/3804453‑3804639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`