Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,804,546	T→C	E50G (GAG→GGG)	rfaG ←	glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,804,546	0	T	C	99.6%	787.0 / ‑4.8	241	E50G (GAG→GGG)	rfaG	glucosyltransferase I
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (116/124); total (117/124)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01

Only 100 of 244 total aligned reads displayed.

GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATA  >  NC_000913/3804456‑3804635

ggATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCa                                                                                         >  1:1150353/1‑93 (MQ=255)

ggATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCa                                                                                         <  1:4808461/93‑1 (MQ=255)

    gCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATg                                                                                     >  1:813141/1‑93 (MQ=255)

     ctctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGc                                                                                    <  2:4823680/93‑1 (MQ=255)

      tctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCt                                                                                   >  2:4929875/1‑93 (MQ=255)

      tctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCt                                                                                   >  1:433482/1‑93 (MQ=255)

      tctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCt                                                                                   >  1:5919185/1‑93 (MQ=255)

      tctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCt                                                                                   >  1:4956204/1‑93 (MQ=255)

       ctTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCtt                                                                                  <  1:2470397/93‑1 (MQ=255)

        tttGATATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:825464/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  2:3194004/93‑1 (MQ=255)

        tttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCttt                                                                                 <  1:2790004/93‑1 (MQ=255)

         ttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTc                                                                                <  2:5671511/93‑1 (MQ=255)

         ttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTc                                                                                >  2:452840/1‑93 (MQ=255)

          tGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCg                                                                               <  1:6204840/93‑1 (MQ=255)

          tGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCg                                                                               >  2:4112693/1‑93 (MQ=255)

          tGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCg                                                                               <  2:2534786/93‑1 (MQ=255)

          tGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGCTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCg                                                                               <  1:4434732/93‑1 (MQ=255)

           gagaTGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCgg                                                                              >  2:5165581/1‑93 (MQ=255)

           gagaTGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCgg                                                                              <  1:3596791/93‑1 (MQ=255)

             gatgatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCggga                                                                            >  2:3450440/1‑92 (MQ=255)

             gatgatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGc                                                                            >  2:3769848/1‑93 (MQ=255)

                gatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAt                                                                         <  1:561914/93‑1 (MQ=255)

                gatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAt                                                                         <  2:4263345/93‑1 (MQ=255)

                gatTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAt                                                                         <  2:3120945/93‑1 (MQ=255)

                 atTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATc                                                                        <  2:5377313/93‑1 (MQ=255)

                 atTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATc                                                                        <  2:2679702/93‑1 (MQ=255)

                         cccAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTccc                                                                >  1:5016941/1‑93 (MQ=255)

                          ccAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCa                                                               <  1:1802939/93‑1 (MQ=255)

                           cAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAc                                                              <  1:404480/93‑1 (MQ=255)

                           cAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAc                                                              <  1:4155717/93‑1 (MQ=255)

                            aGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACg                                                             >  1:6233261/1‑93 (MQ=255)

                            aGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACg                                                             >  2:3690444/1‑93 (MQ=255)

                             ggCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGa                                                            >  2:5110501/1‑93 (MQ=255)

                               cATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACt                                                          <  1:1060210/93‑1 (MQ=255)

                                  aataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACtgtg                                                       >  1:2669997/1‑93 (MQ=255)

                                  aataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACtgtg                                                       >  1:699837/1‑93 (MQ=255)

                                   atatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTgtgt                                                      >  2:1066728/1‑93 (MQ=255)

                                    tatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGta                                                     <  1:4452373/93‑1 (MQ=255)

                                     atTCTGCAGTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAAGAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtat                                                    <  1:2081744/93‑1 (MQ=255)

                                      ttCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtata                                                   <  1:1243222/93‑1 (MQ=255)

                                       tCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatat                                                  <  2:4325938/93‑1 (MQ=255)

                                       tCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatat                                                  <  1:2017900/93‑1 (MQ=255)

                                       tCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatat                                                  <  1:3067613/93‑1 (MQ=255)

                                        cTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                 >  1:1756615/1‑93 (MQ=255)

                                            aTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCg                                             >  1:5687831/1‑93 (MQ=255)

                                            aTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCg                                             >  1:932494/1‑93 (MQ=255)

                                             ttGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGa                                            >  2:1759623/1‑93 (MQ=255)

                                             ttGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGa                                            >  1:2004008/1‑93 (MQ=255)

                                              tGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGaa                                           <  1:2618481/93‑1 (MQ=255)

                                               gCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAc                                          <  2:1586554/93‑1 (MQ=255)

                                               gCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAc                                          <  1:2635013/93‑1 (MQ=255)

                                                cGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACa                                         <  2:6296530/93‑1 (MQ=255)

                                                  tCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtg                                       >  1:5146132/1‑93 (MQ=255)

                                                      tggttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtggtgg                                   >  2:5791624/1‑93 (MQ=255)

                                                       ggttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGc                                  >  2:323859/1‑93 (MQ=255)

                                                       ggttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGc                                  >  2:5940851/1‑93 (MQ=255)

                                                         ttggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGccc                                <  1:3346099/93‑1 (MQ=255)

                                                          tggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGcccc                               <  2:1841337/93‑1 (MQ=255)

                                                           ggtATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCg                              <  1:2131956/93‑1 (MQ=255)

                                                             tATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTg                            <  1:223110/93‑1 (MQ=255)

                                                             tATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTg                            >  1:6015391/1‑93 (MQ=255)

                                                               tGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCg                          <  2:3979320/93‑1 (MQ=255)

                                                               tGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCg                          >  1:4320092/1‑93 (MQ=255)

                                                               tGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCg                          >  2:4684944/1‑93 (MQ=255)

                                                                 ggACTTAACTGGCACCTGAATAAGCCCAAGTGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGc                        <  2:6190266/93‑1 (MQ=255)

                                                                 ggACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGc                        >  1:5375849/1‑93 (MQ=255)

                                                                 ggACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGc                        <  1:2407641/93‑1 (MQ=255)

                                                                 ggACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGc                        <  1:3380681/93‑1 (MQ=255)

                                                                 ggACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGc                        >  1:5200598/1‑93 (MQ=255)

                                                                   aCTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCaa                      <  2:927059/93‑1 (MQ=255)

                                                                    cTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAAc                     >  2:2959004/1‑93 (MQ=255)

                                                                    cTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAAc                     <  1:5499769/93‑1 (MQ=255)

                                                                     ttAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACt                    <  1:3994047/93‑1 (MQ=255)

                                                                     ttAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACt                    >  2:5532573/1‑93 (MQ=255)

                                                                     ttAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACt                    <  1:1584426/93‑1 (MQ=255)

                                                                     ttAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCACCGTGCGGCAACt                    >  2:1026831/1‑93 (MQ=255)

                                                                      tAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTg                   >  1:3238429/1‑93 (MQ=255)

                                                                       aaCTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGt                  <  1:5292406/93‑1 (MQ=255)

                                                                        aCTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGtt                 <  1:1109763/93‑1 (MQ=255)

                                                                           ggCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAt              <  1:355042/93‑1 (MQ=255)

                                                                           ggCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAt              <  2:3791060/93‑1 (MQ=255)

                                                                           ggCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAt              <  1:1529051/93‑1 (MQ=255)

                                                                           ggCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAt              >  2:3371049/1‑93 (MQ=255)

                                                                               ccTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCaa          >  1:3742024/1‑93 (MQ=255)

                                                                                cTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt         <  2:1970252/93‑1 (MQ=255)

                                                                                cTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt         <  2:4677982/93‑1 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATGCCCGATCATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        <  1:3536681/93‑1 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        >  1:1596042/1‑93 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        <  1:4859307/93‑1 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        <  2:1276312/93‑1 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        <  2:2081333/93‑1 (MQ=255)

                                                                                 tGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATg        >  2:224506/1‑93 (MQ=255)

                                                                                   aaTAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATgcg      <  2:699837/93‑1 (MQ=255)

                                                                                   aaTAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGCGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATgcg      >  2:2335182/1‑93 (MQ=255)

                                                                                    aTAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATgcgc     <  2:2111606/93‑1 (MQ=255)

                                                                                     tAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCa    <  2:1300945/93‑1 (MQ=255)

                                                                                      aaGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCTATGCGCAt   <  2:6118498/93‑1 (MQ=255)

                                                                                      aaGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCAt   >  1:1669664/1‑93 (MQ=255)

                                                                                       aGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATa  <  1:4013267/93‑1 (MQ=255)

GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATA  >  NC_000913/3804456‑3804635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  NC_000913/3804453‑3804639

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCC                                                                                               <  SRR4302533.2528248/94‑1 (MQ=60)

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCC                                                                                               <  SRR4302533.3630991/94‑1 (MQ=60)

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCC                                                                                               <  SRR4302533.5370681/94‑1 (MQ=60)

 CGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCC                                                                                              <  SRR4302533.3970121/94‑1 (MQ=60)

  GGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCA                                                                                             >  SRR4302533.1157244/1‑94 (MQ=60)

   GGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAA                                                                                            <  SRR4302533.4837572/94‑1 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          <  SRR4302533.394180/94‑1 (MQ=60)

     ATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAAT                                                                                          <  SRR4302533.4056935/94‑1 (MQ=60)

      TGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATG                                                                                         <  SRR4302533.4909846/94‑1 (MQ=60)

      TGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATG                                                                                         >  SRR4302533.817901/1‑94 (MQ=60)

      TGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATG                                                                                         <  SRR4302533.1601744/94‑1 (MQ=60)

        CTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCT                                                                                       >  SRR4302533.436067/1‑94 (MQ=60)

        CTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCT                                                                                       >  SRR4302533.4986227/1‑94 (MQ=60)

        CTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCT                                                                                       >  SRR4302533.5954964/1‑94 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     >  SRR4302533.156642/1‑94 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302533.2485203/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302533.2485634/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302533.963981/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302533.4465745/94‑1 (MQ=60)

          CTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTT                                                                                     <  SRR4302533.4019388/94‑1 (MQ=60)

           TTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTC                                                                                    <  SRR4302533.2807160/94‑1 (MQ=60)

            TTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCG                                                                                   >  SRR4302533.4588638/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  >  SRR4302533.5213120/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  >  SRR4302533.4293264/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  <  SRR4302533.6242051/94‑1 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  >  SRR4302533.1960986/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  >  SRR4302533.6256429/1‑94 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  <  SRR4302533.2785354/94‑1 (MQ=60)

             TGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGCTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGG                                                                                  <  SRR4302533.4461540/94‑1 (MQ=60)

              GAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGG                                                                                 <  SRR4302533.3618923/94‑1 (MQ=60)

               AGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGC                                                                                <  SRR4302533.2427254/94‑1 (MQ=60)

                GATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCA                                                                               <  SRR4302533.5432225/94‑1 (MQ=60)

                 ATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAA                                                                              >  SRR4302533.5143164/1‑94 (MQ=60)

                  TGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAT                                                                             >  SRR4302533.2042830/1‑94 (MQ=60)

                  TGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAAT                                                                             >  SRR4302533.2657791/1‑94 (MQ=60)

                   GATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATC                                                                            <  SRR4302533.565246/94‑1 (MQ=60)

                   GATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATC                                                                            >  SRR4302533.4071832/1‑94 (MQ=60)

                    ATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCG                                                                           >  SRR4302533.5029368/1‑94 (MQ=60)

                     TTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGC                                                                          >  SRR4302533.4713668/1‑94 (MQ=60)

                       TTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTCAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCT                                                                        <  SRR4302533.3405742/94‑1 (MQ=60)

                        TGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTT                                                                       <  SRR4302533.1979677/94‑1 (MQ=60)

                          TACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCC                                                                     >  SRR4302533.203293/1‑94 (MQ=60)

                          TACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCC                                                                     >  SRR4302533.5702611/1‑94 (MQ=60)

                           ACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCC                                                                    >  SRR4302533.5047333/1‑94 (MQ=60)

                           ACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCC                                                                    <  SRR4302533.5577275/94‑1 (MQ=60)

                             CCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCAC                                                                  <  SRR4302533.1813915/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 >  SRR4302533.3737610/1‑94 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302533.1004907/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302533.406915/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 <  SRR4302533.4180897/94‑1 (MQ=60)

                              CAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACG                                                                 >  SRR4302533.6270584/1‑94 (MQ=60)

                               AGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGA                                                                >  SRR4302533.4928904/1‑94 (MQ=60)

                               AGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGA                                                                >  SRR4302533.5380360/1‑94 (MQ=60)

                               AGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGA                                                                >  SRR4302533.6242034/1‑94 (MQ=60)

                               AGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGA                                                                >  SRR4302533.3367888/1‑94 (MQ=60)

                                  CATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTG                                                             <  SRR4302533.1066443/94‑1 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302533.2173332/1‑94 (MQ=60)

                                   ATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGT                                                            >  SRR4302533.783743/1‑94 (MQ=60)

                                    TAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTG                                                           >  SRR4302533.2686375/1‑94 (MQ=60)

                                    TAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTG                                                           >  SRR4302533.703980/1‑94 (MQ=60)

                                     AATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGT                                                          >  SRR4302533.3660930/1‑94 (MQ=60)

                                     AATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGT                                                          >  SRR4302533.2026003/1‑94 (MQ=60)

                                      ATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTA                                                         >  SRR4302533.1651324/1‑94 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        <  SRR4302533.4479292/94‑1 (MQ=60)

                                       TATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTAT                                                        <  SRR4302533.2562762/94‑1 (MQ=60)

                                        ATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATA                                                       >  SRR4302533.4144132/1‑94 (MQ=60)

                                        ATTCTGCAGTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAAGAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATA                                                       <  SRR4302533.2094556/94‑1 (MQ=60)

                                         TTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATAT                                                      <  SRR4302533.1250645/94‑1 (MQ=60)

                                          TCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATA                                                     <  SRR4302533.2030334/94‑1 (MQ=60)

                                          TCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATA                                                     <  SRR4302533.3086453/94‑1 (MQ=60)

                                          TCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATA                                                     >  SRR4302533.1767318/1‑94 (MQ=60)

                                          TCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATA                                                     >  SRR4302533.3348984/1‑94 (MQ=60)

                                          TCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATA                                                     <  SRR4302533.2645996/94‑1 (MQ=60)

                                           CTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATAC                                                    >  SRR4302533.1542542/1‑94 (MQ=60)

                                            TGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACC                                                   >  SRR4302533.6075315/1‑94 (MQ=60)

                                              CATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCG                                                 >  SRR4302533.5722185/1‑94 (MQ=60)

                                              CATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCG                                                 >  SRR4302533.937967/1‑94 (MQ=60)

                                               ATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGA                                                >  SRR4302533.2016349/1‑94 (MQ=60)

                                                 TGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAC                                              >  SRR4302533.2672869/1‑94 (MQ=60)

                                                 TGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAC                                              <  SRR4302533.2634556/94‑1 (MQ=60)

                                                  GCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACA                                             <  SRR4302533.2651209/94‑1 (MQ=60)

                                                   CGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAT                                            <  SRR4302533.36121/94‑1 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATG                                           >  SRR4302533.5935830/1‑94 (MQ=60)

                                                    GTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATG                                           >  SRR4302533.5177232/1‑94 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         >  SRR4302533.1196418/1‑94 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         >  SRR4302533.113400/1‑94 (MQ=60)

                                                      CCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGT                                         <  SRR4302533.5260171/94‑1 (MQ=60)

                                                       CATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTG                                        >  SRR4302533.5592833/1‑94 (MQ=60)

                                                        ATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGG                                       <  SRR4302533.1709075/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302533.2271704/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302533.3366772/94‑1 (MQ=60)

                                                            TTGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCC                                   <  SRR4302533.2881845/94‑1 (MQ=60)

                                                             TGGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCG                                  >  SRR4302533.892150/1‑94 (MQ=60)

                                                              GGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGT                                 <  SRR4302533.2145104/94‑1 (MQ=60)

                                                              GGTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGT                                 <  SRR4302533.3209353/94‑1 (MQ=60)

                                                               GTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTG                                >  SRR4302533.6051843/1‑94 (MQ=60)

                                                               GTATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTG                                <  SRR4302533.4454049/94‑1 (MQ=60)

                                                                TATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGC                               <  SRR4302533.224502/94‑1 (MQ=60)

                                                                 ATGGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCG                              >  SRR4302533.4346111/1‑94 (MQ=60)

                                                                   GGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGC                            >  SRR4302533.5408158/1‑94 (MQ=60)

                                                                   GGGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGC                            >  SRR4302533.5231948/1‑94 (MQ=60)

                                                                    GGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCA                           <  SRR4302533.3401551/94‑1 (MQ=60)

                                                                    GGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCA                           <  SRR4302533.3571688/94‑1 (MQ=60)

                                                                    GGACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCA                           <  SRR4302533.2422511/94‑1 (MQ=60)

                                                                    GGACTTAACTGGCACCTCAATAAGCCCAAGTGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATGCCCGAACATGGTGGCCCCGTGCGGCA                           <  SRR4302533.2102320/94‑1 (MQ=60)

                                                                      ACTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAAC                         <  SRR4302533.1112196/94‑1 (MQ=60)

                                                                       CTTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACT                        <  SRR4302533.5532946/94‑1 (MQ=60)

                                                                        TTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTG                       >  SRR4302533.3258385/1‑94 (MQ=60)

                                                                        TTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTG                       >  SRR4302533.354321/1‑94 (MQ=60)

                                                                        TTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTG                       <  SRR4302533.4018367/94‑1 (MQ=60)

                                                                        TTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTG                       >  SRR4302533.217532/1‑94 (MQ=60)

                                                                        TTAACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTG                       <  SRR4302533.1593972/94‑1 (MQ=60)

                                                                          AACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTT                     <  SRR4302533.5324088/94‑1 (MQ=60)

                                                                          AACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTT                     >  SRR4302533.6311756/1‑94 (MQ=60)

                                                                           ACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTG                    <  SRR4302533.5134444/94‑1 (MQ=60)

                                                                           ACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTG                    <  SRR4302533.1116347/94‑1 (MQ=60)

                                                                           ACTGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTG                    >  SRR4302533.434852/1‑94 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302533.1631511/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302533.5970507/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302533.1447609/94‑1 (MQ=60)

                                                                             TGGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGAT                  <  SRR4302533.3327833/94‑1 (MQ=60)

                                                                              GGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATG                 <  SRR4302533.357207/94‑1 (MQ=60)

                                                                              GGCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATG                 <  SRR4302533.1538247/94‑1 (MQ=60)

                                                                               GCACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGC                <  SRR4302533.4398566/94‑1 (MQ=60)

                                                                                CACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCA               >  SRR4302533.4869438/1‑94 (MQ=60)

                                                                                 ACCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAA              >  SRR4302533.3764973/1‑94 (MQ=60)

                                                                                  CCTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAT             >  SRR4302533.1780134/1‑94 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            >  SRR4302533.1605701/1‑94 (MQ=60)

                                                                                   CTGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATG            <  SRR4302533.1168936/94‑1 (MQ=60)

                                                                                    TGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATGCCCGATCATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGC           <  SRR4302533.3558480/94‑1 (MQ=60)

                                                                                    TGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGC           >  SRR4302533.5949742/1‑94 (MQ=60)

                                                                                    TGAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGC           <  SRR4302533.4888762/94‑1 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGTTGGCCCCGTGCGGCAACTGTTGATGCAATGCG          <  SRR4302533.787806/94‑1 (MQ=60)

                                                                                     GAATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCG          <  SRR4302533.4019224/94‑1 (MQ=60)

                                                                                       ATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCA        >  SRR4302533.2835141/1‑94 (MQ=60)

                                                                                       ATAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCA        <  SRR4302533.5359483/94‑1 (MQ=60)

                                                                                        TAAGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCAT       >  SRR4302533.1679751/1‑94 (MQ=60)

                                                                                          AGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAA     <  SRR4302533.4037702/94‑1 (MQ=60)

                                                                                          AGCCCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAA     >  SRR4302533.5649447/1‑94 (MQ=60)

                                                                                             CCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  <  SRR4302533.2306791/94‑1 (MQ=60)

GCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGT  >  NC_000913/3804453‑3804639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`