Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,816,555 (G)5→6 coding (55/618 nt) b3646 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,816,5501.G99.2% 1583.4 / 2.5 496coding (50/618 nt)b3646hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/4);  new base G (166/325);  total (167/329)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 573 total aligned reads displayed.

CACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGT  >  NC_000913/3816458‑3816637
                                                                                             |                                                                                         
cacaAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAc                                                                                            <  1:1128574/93‑1 (MQ=255)
cacaAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAc                                                                                            >  1:6612917/1‑93 (MQ=255)
 acaAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑g                                                                                         <  1:1648957/93‑1 (MQ=255)
   aaaTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑ggg                                                                                       <  1:8104520/93‑1 (MQ=255)
   aaaTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑ggg                                                                                       <  1:5591605/93‑1 (MQ=255)
    aaTCGCTGCCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑gggg                                                                                      <  1:946261/93‑1 (MQ=255)
    aaTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑gggg                                                                                      <  2:672796/93‑1 (MQ=255)
    aaTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑gggg                                                                                      >  1:2402607/1‑93 (MQ=255)
     aTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑ggggg                                                                                     <  2:9936011/93‑1 (MQ=255)
     aTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑ggggg                                                                                     >  1:2029142/1‑93 (MQ=255)
      tCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑gggggg                                                                                    <  2:2163260/93‑2 (MQ=255)
       cGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑ggggggc                                                                                   <  1:8430360/93‑3 (MQ=255)
           aCCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTg                                                                               <  1:7360799/93‑1 (MQ=255)
           aCCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTg                                                                               >  1:4277563/1‑93 (MQ=255)
             ccTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggc                                                                             <  1:2197469/93‑1 (MQ=255)
             ccTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggc                                                                             <  2:904074/93‑1 (MQ=255)
              cTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggcg                                                                            >  1:5884496/1‑93 (MQ=255)
                gCCAGACAGATTTTTAGGGAGAGAACCATGCTTTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggcggc                                                                          <  2:5650351/93‑1 (MQ=255)
                gCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggcggc                                                                          <  2:6377600/93‑1 (MQ=255)
                gCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTggcggc                                                                          <  1:1776741/93‑1 (MQ=255)
                  cagacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCg                                                                        <  2:6568540/93‑1 (MQ=255)
                  cagacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCg                                                                        >  2:4226377/1‑93 (MQ=255)
                  cagacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCg                                                                        >  1:4422871/1‑93 (MQ=255)
                   agacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCgg                                                                       <  1:7959270/93‑1 (MQ=255)
                    gacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGga                                                                      <  2:7863787/93‑1 (MQ=255)
                    gacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGga                                                                      <  2:5376375/93‑1 (MQ=255)
                     acagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGgac                                                                     >  2:4431144/1‑93 (MQ=255)
                      cagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGgacg                                                                    <  2:3681368/93‑1 (MQ=255)
                       agaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGgacga                                                                   <  1:7842004/93‑1 (MQ=255)
                       agaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGgacga                                                                   <  1:288089/93‑1 (MQ=255)
                       agaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGgacga                                                                   <  2:1763268/93‑1 (MQ=255)
                         aTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCGGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGacgacg                                                                 <  2:4184821/93‑1 (MQ=255)
                         aTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGacgacg                                                                 <  1:7225479/93‑1 (MQ=255)
                          tttttAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACgg                                                                <  2:6157370/93‑1 (MQ=255)
                          tttttAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACgg                                                                <  2:1987739/93‑1 (MQ=255)
                          tttttAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACgg                                                                <  2:9294473/93‑1 (MQ=255)
                           ttttAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGgc                                                               <  1:8258854/93‑1 (MQ=255)
                              tAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCa                                                            <  1:3124044/93‑1 (MQ=255)
                              tAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCa                                                            <  1:3900802/93‑1 (MQ=255)
                              tAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCa                                                            <  2:3276566/93‑1 (MQ=255)
                               aGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCAt                                                           <  2:3932158/93‑1 (MQ=255)
                               aGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCAt                                                           >  1:4792154/1‑93 (MQ=255)
                               aGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCAt                                                           <  1:255054/93‑1 (MQ=255)
                               aGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCAt                                                           <  2:4922929/93‑1 (MQ=255)
                                ggaagagaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATg                                                          <  2:5855291/90‑1 (MQ=255)
                                 ggAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATgg                                                         <  1:4525572/93‑1 (MQ=255)
                                  gagagaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGa                                                        <  2:3615185/93‑1 (MQ=255)
                                   agagaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGAt                                                       <  2:7301235/93‑1 (MQ=255)
                                    gagaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATa                                                      <  2:2298556/93‑1 (MQ=255)
                                    gagaACCATGCTGTTACACATTTTGAATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATa                                                      <  2:6288671/93‑1 (MQ=255)
                                      gaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACa                                                    >  1:6734805/1‑93 (MQ=255)
                                      gaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACa                                                    <  2:8837653/93‑1 (MQ=255)
                                      gaACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACa                                                    <  1:536983/93‑1 (MQ=255)
                                        aCCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑AGCTGGCGGCCGGACGACGGCGCATGGATACAtt                                                  >  1:1472636/1‑93 (MQ=255)
                                         ccATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACAttt                                                 >  1:687531/1‑93 (MQ=255)
                                          cATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTg                                                >  1:2684669/1‑93 (MQ=255)
                                           aTGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTgg                                               >  2:1164732/1‑93 (MQ=255)
                                           aTGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTgg                                               >  2:3823965/1‑93 (MQ=255)
                                            tGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGc                                              >  1:3681177/1‑93 (MQ=255)
                                             gCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGc                                              <  1:8884058/93‑1 (MQ=255)
                                             gCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCAAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCg                                             >  2:6907002/1‑93 (MQ=255)
                                               tGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTa                                           >  2:8760493/1‑93 (MQ=255)
                                                  tACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAatt                                        >  2:7757593/1‑93 (MQ=255)
                                                     acaTTTTTTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattatt                                     <  2:7611810/93‑1 (MQ=255)
                                                     acaTTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattatt                                     <  1:8651522/93‑1 (MQ=255)
                                                      caTTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattatta                                    <  2:1558613/93‑1 (MQ=255)
                                                       aTTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattattat                                   <  2:7140293/93‑1 (MQ=255)
                                                       aTTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattattat                                   >  2:9471129/1‑93 (MQ=255)
                                                        ttttGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattattatt                                  <  1:6264699/93‑1 (MQ=255)
                                                        ttttGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAattattatt                                  >  2:4757047/1‑93 (MQ=255)
                                                         tttGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTg                                 <  1:503134/93‑1 (MQ=255)
                                                           tGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCg                               <  2:2804677/93‑1 (MQ=255)
                                                            gTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGa                              <  1:9653657/93‑1 (MQ=255)
                                                            gTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGa                              <  1:5111305/93‑1 (MQ=255)
                                                             tATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAc                             <  1:1007438/93‑1 (MQ=255)
                                                             tATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAc                             <  2:9869597/93‑1 (MQ=255)
                                                             tATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAc                             <  2:514129/93‑1 (MQ=255)
                                                             gaTTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAc                             <  1:2096975/92‑1 (MQ=255)
                                                                 tggttggCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGcc                         <  1:5538849/93‑1 (MQ=255)
                                                                    ttggCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCAcc                      <  2:3243102/93‑1 (MQ=255)
                                                                     tggCATTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCg                     >  2:8317477/1‑93 (MQ=255)
                                                                         aTTCCTGCCGAAGCCATGAC‑‑GGGGGCCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGCCCGCCACCGCAAt                 <  2:1320118/93‑1 (MQ=255)
                                                                         aTTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAt                 <  2:8483386/93‑1 (MQ=255)
                                                                         aTTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAt                 <  1:1570304/93‑1 (MQ=255)
                                                                         aTTACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAt                 <  2:8102692/93‑1 (MQ=255)
                                                                          ttACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAtt                >  1:6048402/1‑93 (MQ=255)
                                                                          ttACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAtt                <  2:8038824/93‑1 (MQ=255)
                                                                           tACTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTg               <  2:9618863/93‑1 (MQ=255)
                                                                            aCTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTgg              <  2:3442500/93‑1 (MQ=255)
                                                                            aCTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTgg              <  1:356058/93‑1 (MQ=255)
                                                                            aCTGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTgg              <  2:4514835/93‑1 (MQ=255)
                                                                              tGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCg            <  1:9077338/93‑1 (MQ=255)
                                                                              tGCCGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCg            >  1:5764113/1‑93 (MQ=255)
                                                                                ccGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGa          <  1:401642/93‑1 (MQ=255)
                                                                                 cGAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAg         <  1:1261976/93‑1 (MQ=255)
                                                                                  gAAGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAgg        >  2:2622008/1‑93 (MQ=255)
                                                                                    aGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGt      <  1:9985971/93‑1 (MQ=255)
                                                                                    aGCCATGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGt      >  2:5484191/1‑93 (MQ=255)
                                                                                     gCCATGACGGGGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGt      <  1:9337208/93‑1 (MQ=255)
                                                                                        aTGACG‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGt  <  1:3461302/93‑1 (MQ=255)
                                                                                             |                                                                                         
CACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGGG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGT  >  NC_000913/3816458‑3816637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 295 total aligned reads displayed.
AATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTC  >  NC_000913/3816462‑3816648
                                                                                                |                                                                                              
AATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G                                                                                                <  SRR4302540.956122/94‑1 (MQ=60)
AATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G                                                                                                <  SRR4302540.9533114/94‑1 (MQ=60)
AATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G                                                                                                >  SRR4302540.2051048/1‑94 (MQ=60)
 ATCGCTCCCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑G                                                                                              <  SRR4302540.7399588/94‑1 (MQ=60)
 ATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑G                                                                                              >  SRR4302540.5039976/1‑94 (MQ=60)
 ATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑G                                                                                              <  SRR4302540.1973833/94‑1 (MQ=60)
  TCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑Ggc                                                                                              <  SRR4302540.3831339/94‑3 (MQ=60)
   CGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑Ggag                                                                                             >  SRR4302540.5907743/1‑91 (MQ=60)
    GCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGC                                                                                            >  SRR4302540.1649447/1‑94 (MQ=60)
    GCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGC                                                                                            >  SRR4302540.3999596/1‑94 (MQ=60)
      TACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTG                                                                                          >  SRR4302540.1593581/1‑94 (MQ=60)
      TACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTG                                                                                          >  SRR4302540.2583255/1‑94 (MQ=60)
      TACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTG                                                                                          <  SRR4302540.8530413/94‑1 (MQ=60)
      TACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTG                                                                                          >  SRR4302540.4324086/1‑94 (MQ=60)
       ACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGG‑G‑CGCTG                                                                                          <  SRR4302540.1464167/94‑1 (MQ=60)
       ACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGG                                                                                         >  SRR4302540.2074411/1‑94 (MQ=60)
        CCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGC                                                                                        <  SRR4302540.5605405/94‑1 (MQ=60)
        CCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑Ggcgctggg                                                                                        >  SRR4302540.182039/1‑86 (MQ=60)
         CCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCG                                                                                       <  SRR4302540.2221200/94‑1 (MQ=60)
         CCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCG                                                                                       <  SRR4302540.8234212/94‑1 (MQ=60)
         CCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCG                                                                                       >  SRR4302540.5949511/1‑94 (MQ=60)
          CTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGCAGCCATGACG‑GGGG‑G‑CGCTGGCGG                                                                                      >  SRR4302540.5103047/1‑94 (MQ=60)
            GCCAGACAGGTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    >  SRR4302540.413646/1‑94 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    <  SRR4302540.1796033/94‑1 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    <  SRR4302540.1850943/94‑1 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    >  SRR4302540.31745/1‑94 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    >  SRR4302540.6779635/1‑94 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    <  SRR4302540.3646815/94‑1 (MQ=60)
            GCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCC                                                                                    <  SRR4302540.6221169/94‑1 (MQ=60)
             CCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCG                                                                                   >  SRR4302540.4470793/1‑94 (MQ=60)
             CCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCG                                                                                   <  SRR4302540.259486/94‑1 (MQ=60)
             CCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCG                                                                                   >  SRR4302540.8962231/1‑94 (MQ=60)
              CAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCA‑CACCGGGGG‑G‑CGCCGGCGGCCGG                                                                                  >  SRR4302540.8908125/1‑94 (MQ=60)
               AGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGA                                                                                 <  SRR4302540.8052037/94‑1 (MQ=60)
                GACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAG                                                                                >  SRR4302540.6856607/1‑94 (MQ=60)
                GACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAC                                                                                <  SRR4302540.9658040/94‑1 (MQ=60)
                GACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAC                                                                                <  SRR4302540.7411626/94‑1 (MQ=60)
                GACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAC                                                                                <  SRR4302540.1234721/94‑1 (MQ=60)
                GACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAC                                                                                <  SRR4302540.1339760/94‑1 (MQ=60)
                GACAGATTTTGAGTGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGAC                                                                                <  SRR4302540.6927669/94‑1 (MQ=60)
                  CAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGA                                                                              >  SRR4302540.7185502/1‑94 (MQ=60)
                   AGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGAC                                                                             >  SRR4302540.5988523/1‑94 (MQ=60)
                   AGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGAC                                                                             <  SRR4302540.7933640/94‑1 (MQ=60)
                   AGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGAC                                                                             <  SRR4302540.290932/94‑1 (MQ=60)
                    GATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            >  SRR4302540.6050639/1‑94 (MQ=60)
                    GATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            <  SRR4302540.3106080/94‑1 (MQ=60)
                    GATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            <  SRR4302540.355249/94‑1 (MQ=60)
                    GATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAACCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            >  SRR4302540.3857587/1‑94 (MQ=60)
                    GATTTTTAGGAAGAGTACCATGCTGTTACACATTTTGTATTTGGTTGACATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            <  SRR4302540.7183831/94‑1 (MQ=60)
                    AATTTTTAGGGAGAGAACCATGCGGTTCCACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACG                                                                            <  SRR4302540.1022125/94‑1 (MQ=60)
                     ATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGG                                                                           <  SRR4302540.7306045/94‑1 (MQ=60)
                      TTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGC                                                                          <  SRR4302540.6520142/94‑1 (MQ=60)
                      TTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGC                                                                          <  SRR4302540.513228/94‑1 (MQ=60)
                      TTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGC                                                                          <  SRR4302540.6520141/94‑1 (MQ=60)
                       TTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCG                                                                         <  SRR4302540.501880/94‑1 (MQ=60)
                       TTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCG                                                                         <  SRR4302540.5283664/94‑1 (MQ=60)
                       TTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCG                                                                         <  SRR4302540.5761096/94‑1 (MQ=60)
                       TTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCG                                                                         <  SRR4302540.9358374/94‑1 (MQ=60)
                       TTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCG                                                                         <  SRR4302540.8354830/94‑1 (MQ=60)
                         TTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCA                                                                       <  SRR4302540.1704861/94‑1 (MQ=60)
                         TTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCA                                                                       <  SRR4302540.853206/94‑1 (MQ=60)
                          TAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCAT                                                                      <  SRR4302540.7959096/94‑1 (MQ=60)
                          TAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCAT                                                                      <  SRR4302540.4676279/94‑1 (MQ=60)
                          TAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCAT                                                                      <  SRR4302540.8039737/94‑1 (MQ=60)
                          TAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCAT                                                                      <  SRR4302540.3943123/94‑1 (MQ=60)
                          TAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCAT                                                                      <  SRR4302540.8233727/94‑1 (MQ=60)
                           AGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATG                                                                     >  SRR4302540.8430516/1‑94 (MQ=60)
                           AGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATG                                                                     <  SRR4302540.2494280/94‑1 (MQ=60)
                           AGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATG                                                                     <  SRR4302540.257527/94‑1 (MQ=60)
                             GGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGA                                                                   <  SRR4302540.2815020/94‑1 (MQ=60)
                              GAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGAT                                                                  <  SRR4302540.6758981/94‑1 (MQ=60)
                              GAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGAT                                                                  <  SRR4302540.1544158/94‑1 (MQ=60)
                              GAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGAT                                                                  <  SRR4302540.91404/94‑1 (MQ=60)
                               AGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATA                                                                 >  SRR4302540.112178/1‑94 (MQ=60)
                                GAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATAC                                                                >  SRR4302540.4842445/1‑94 (MQ=60)
                                GAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATAC                                                                >  SRR4302540.2754557/1‑94 (MQ=60)
                                GAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATAC                                                                <  SRR4302540.4438073/94‑1 (MQ=60)
                                GAGAACCAGGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATAC                                                                <  SRR4302540.2690161/94‑1 (MQ=60)
                                 AGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACA                                                               >  SRR4302540.6809182/1‑94 (MQ=60)
                                  GAACCATTCTGTTTCACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACAT                                                              <  SRR4302540.5665282/94‑1 (MQ=60)
                                  AAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACAT                                                              <  SRR4302540.4180797/94‑1 (MQ=60)
                                   AACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATT                                                             <  SRR4302540.2057458/94‑1 (MQ=60)
                                   AACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑AGCTGGCGGCCGGACGACGGCGCATGGATACATT                                                             >  SRR4302540.1488737/1‑94 (MQ=60)
                                    ACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTT                                                            >  SRR4302540.694913/1‑94 (MQ=60)
                                    ACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTT                                                            >  SRR4302540.783537/1‑94 (MQ=60)
                                     CCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTG                                                           <  SRR4302540.2190916/94‑1 (MQ=60)
                                     CCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGG                                                          <  SRR4302540.6499281/94‑1 (MQ=60)
                                      CATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTTG                                                          >  SRR4302540.8192610/1‑94 (MQ=60)
                                      CATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGG                                                          <  SRR4302540.6946754/94‑1 (MQ=60)
                                       ATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGC                                                         >  SRR4302540.3709981/1‑94 (MQ=60)
                                       ATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGC                                                         >  SRR4302540.3721412/1‑94 (MQ=60)
                                        TGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCG                                                        <  SRR4302540.3085981/94‑1 (MQ=60)
                                         GCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCG                                                        <  SRR4302540.8986893/94‑1 (MQ=60)
                                         GCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGT                                                       <  SRR4302540.4525506/94‑1 (MQ=60)
                                          CTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTA                                                      >  SRR4302540.5632237/1‑94 (MQ=60)
                                           TGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAA                                                     >  SRR4302540.4500781/1‑94 (MQ=60)
                                           TGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAA                                                     >  SRR4302540.1299215/1‑94 (MQ=60)
                                             TTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATT                                                   >  SRR4302540.8981205/1‑94 (MQ=60)
                                             TTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATT                                                   <  SRR4302540.1618770/94‑1 (MQ=60)
                                             TTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATT                                                   >  SRR4302540.815752/1‑94 (MQ=60)
                                              TACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTA                                                  <  SRR4302540.9904690/94‑1 (MQ=60)
                                               tcgCATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.8598095/91‑1 (MQ=60)
                                               tcactttttgtatgtggtagacagTACTGCCAAAGCCATGAC‑‑GGGG‑GTCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.5626203/70‑1 (MQ=60)
                                               ACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.9577364/94‑1 (MQ=60)
                                               ACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.1223762/94‑1 (MQ=60)
                                               ACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.1661729/94‑1 (MQ=60)
                                               ACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTAT                                                 <  SRR4302540.2439928/94‑1 (MQ=60)
                                                 agatgtggtagTTGGTTGGCATTACTGCGAAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               <  SRR4302540.2884577/83‑1 (MQ=60)
                                                 CCATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               <  SRR4302540.3968805/94‑1 (MQ=60)
                                                 ACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               <  SRR4302540.8479680/94‑1 (MQ=60)
                                                 ACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               <  SRR4302540.5597213/94‑1 (MQ=60)
                                                 ACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               >  SRR4302540.6266531/1‑94 (MQ=60)
                                                 ACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA                                               <  SRR4302540.4258317/94‑1 (MQ=60)
                                                  CATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTAT                                              <  SRR4302540.10074145/94‑1 (MQ=60)
                                                  CATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTAT                                              <  SRR4302540.3345713/94‑1 (MQ=60)
                                                   ATTTTGTATTTGGTTGGCATTCCTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATT                                             <  SRR4302540.3530956/94‑1 (MQ=60)
                                                   ATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATT                                             >  SRR4302540.9842169/1‑94 (MQ=60)
                                                   ATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATT                                             >  SRR4302540.867902/1‑94 (MQ=60)
                                                    TTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTG                                            <  SRR4302540.5436967/94‑1 (MQ=60)
                                                    TTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTG                                            <  SRR4302540.6334393/94‑1 (MQ=60)
                                                    TTTGGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTG                                            <  SRR4302540.5436968/94‑1 (MQ=60)
                                                     TTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGC                                           <  SRR4302540.8132857/94‑1 (MQ=60)
                                                     TTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGC                                           <  SRR4302540.508527/94‑1 (MQ=60)
                                                       TGTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGA                                         <  SRR4302540.2149691/94‑1 (MQ=60)
                                                        gtttgtgggTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.7092322/85‑1 (MQ=60)
                                                        gtattggtgTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.9767313/85‑1 (MQ=60)
                                                        GTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.5166633/94‑1 (MQ=60)
                                                        GTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.7470742/94‑1 (MQ=60)
                                                        GTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.9729566/94‑1 (MQ=60)
                                                        GTATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGAC                                        <  SRR4302540.9767314/94‑1 (MQ=60)
                                                         TATTTGGTTGGCATTACTGCCTAAGCAATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACC                                       <  SRR4302540.9501765/94‑1 (MQ=60)
                                                         GATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACC                                       <  SRR4302540.2119489/94‑1 (MQ=60)
                                                          TTTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCG                                      <  SRR4302540.2400460/94‑1 (MQ=60)
                                                          ATTTGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCG                                      <  SRR4302540.9311131/94‑1 (MQ=60)
                                                             TGGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCA                                   <  SRR4302540.5599855/94‑1 (MQ=60)
                                                              GGTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCAC                                  <  SRR4302540.4381770/94‑1 (MQ=60)
                                                               GTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACC                                 <  SRR4302540.2797160/94‑1 (MQ=60)
                                                               GTTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACC                                 >  SRR4302540.7364385/1‑94 (MQ=60)
                                                                TTGGCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCG                                <  SRR4302540.1737405/94‑1 (MQ=60)
                                                                   GCATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAA                             >  SRR4302540.393621/1‑94 (MQ=60)
                                                                    CATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAAT                            <  SRR4302540.5350964/94‑1 (MQ=60)
                                                                     ATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATT                           >  SRR4302540.6115608/1‑94 (MQ=60)
                                                                     ATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATT                           >  SRR4302540.5904362/1‑94 (MQ=60)
                                                                     ATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATT                           <  SRR4302540.1587514/94‑1 (MQ=60)
                                                                     ATTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATT                           <  SRR4302540.4629130/94‑1 (MQ=60)
                                                                      TTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTG                          <  SRR4302540.1775377/94‑1 (MQ=60)
                                                                      TTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTG                          <  SRR4302540.9512144/94‑1 (MQ=60)
                                                                      TTACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTG                          <  SRR4302540.359731/94‑1 (MQ=60)
                                                                       TACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGG                         >  SRR4302540.8476471/1‑94 (MQ=60)
                                                                        ACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGC                        >  SRR4302540.8948259/1‑94 (MQ=60)
                                                                        ACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGC                        <  SRR4302540.359718/94‑1 (MQ=60)
                                                                        ACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGC                        <  SRR4302540.3467319/94‑1 (MQ=60)
                                                                        ACTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGCCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGC                        <  SRR4302540.9038175/94‑1 (MQ=60)
                                                                         CTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGGAGACCGGGGCTGGGTACATTTGGCGTAATTATTATTGGGACCGCCCCCGCAATTGGCG                       >  SRR4302540.2361485/1‑94 (MQ=36)
                                                                         CTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCG                       <  SRR4302540.6000575/94‑1 (MQ=60)
                                                                         CTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCG                       <  SRR4302540.7466682/94‑1 (MQ=60)
                                                                         CTGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCG                       >  SRR4302540.5827759/1‑94 (MQ=60)
                                                                          TGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGG                      <  SRR4302540.586534/94‑1 (MQ=60)
                                                                          TGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGG                      <  SRR4302540.9205873/94‑1 (MQ=60)
                                                                          TGCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGG                      <  SRR4302540.115463/94‑1 (MQ=60)
                                                                           GCCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGA                     <  SRR4302540.3025850/94‑1 (MQ=60)
                                                                            cgaAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAG                    <  SRR4302540.1067135/91‑1 (MQ=60)
                                                                            CCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAG                    <  SRR4302540.405832/94‑1 (MQ=60)
                                                                            CCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAG                    <  SRR4302540.2426739/94‑1 (MQ=60)
                                                                            CCGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAG                    <  SRR4302540.6704683/94‑1 (MQ=60)
                                                                            CCGAAGCCATGACG‑GGGG‑G‑CGCGGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAG                    <  SRR4302540.9283876/94‑1 (MQ=60)
                                                                             CGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   <  SRR4302540.1274926/94‑1 (MQ=60)
                                                                             CGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   <  SRR4302540.4936965/94‑1 (MQ=60)
                                                                             CGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   >  SRR4302540.5677185/1‑94 (MQ=60)
                                                                             CGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   >  SRR4302540.2922365/1‑94 (MQ=60)
                                                                             CGAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   >  SRR4302540.8655351/1‑94 (MQ=60)
                                                                             CGAAGCAATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGG                   <  SRR4302540.3048846/94‑1 (MQ=60)
                                                                              cagGCCATGAC‑‑GGGGCG‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGG                  <  SRR4302540.4433054/91‑1 (MQ=60)
                                                                              GAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGG                  >  SRR4302540.1530985/1‑94 (MQ=60)
                                                                              GAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGG                  <  SRR4302540.139384/94‑1 (MQ=60)
                                                                              GAAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGG                  >  SRR4302540.2442498/1‑94 (MQ=60)
                                                                               AAGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGT                 <  SRR4302540.2186200/94‑1 (MQ=60)
                                                                                AGCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTC                <  SRR4302540.10102388/94‑1 (MQ=60)
                                                                                 GCCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCA               <  SRR4302540.4482125/94‑1 (MQ=60)
                                                                                  CCATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAG              >  SRR4302540.5553726/1‑94 (MQ=60)
                                                                                   CATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGGGGGTCAGT             >  SRR4302540.2236394/1‑94 (MQ=60)
                                                                                    ATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTG            >  SRR4302540.8615630/1‑94 (MQ=60)
                                                                                    ATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTG            >  SRR4302540.4154390/1‑94 (MQ=60)
                                                                                    ATGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTG            <  SRR4302540.3498975/94‑1 (MQ=60)
                                                                                     TGACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGC           <  SRR4302540.5011919/94‑1 (MQ=60)
                                                                                      GACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCG          <  SRR4302540.3897025/94‑1 (MQ=60)
                                                                                      GACG‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCG          <  SRR4302540.8204/94‑1 (MQ=60)
                                                                                        G‑‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGG       >  SRR4302540.2340934/1‑94 (MQ=60)
                                                                                        G‑‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGA       <  SRR4302540.10122535/94‑1 (MQ=60)
                                                                                         cgGGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCG        <  SRR4302540.7067438/92‑1 (MQ=60)
                                                                                            GGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATA     <  SRR4302540.7662004/94‑1 (MQ=60)
                                                                                            GGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATA     >  SRR4302540.4090834/1‑94 (MQ=60)
                                                                                            GGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATA     <  SRR4302540.6030134/94‑1 (MQ=60)
                                                                                             GG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATAT    <  SRR4302540.9586738/94‑1 (MQ=60)
                                                                                             GG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATAT    <  SRR4302540.4250232/94‑1 (MQ=60)
                                                                                             GG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATAT    >  SRR4302540.4782327/1‑94 (MQ=60)
                                                                                             GG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGAGACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATAT    >  SRR4302540.4895447/1‑94 (MQ=60)
                                                                                                G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTC  <  SRR4302540.3164178/94‑1 (MQ=60)
                                                                                                G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTC  <  SRR4302540.6928195/94‑1 (MQ=60)
                                                                                                G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTC  <  SRR4302540.8062000/94‑1 (MQ=60)
                                                                                                |                                                                                              
AATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGAC‑‑GGGG‑G‑CGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTC  >  NC_000913/3816462‑3816648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑