Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,822,652 T→C Y171H (TAC→CAC)  spoU → putative RNA methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,822,6520TC99.5% 1428.9 / ‑4.2 437Y171H (TAC→CAC) spoUputative RNA methylase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (212/223);  total (212/225)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 445 total aligned reads displayed.

ATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA  >  NC_000913/3822562‑3822741
                                                                                          |                                                                                         
  ggtCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCt                                                                                       <  1:1580982/91‑1 (MQ=255)
  gatCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCt                                                                                       >  1:1687580/1‑93 (MQ=255)
    tCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGc                                                                                     <  2:74591/93‑1 (MQ=255)
     cGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCg                                                                                    <  1:8222252/93‑1 (MQ=255)
     cGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCg                                                                                    <  1:6554543/93‑1 (MQ=255)
      ggCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGt                                                                                   >  2:10047549/1‑93 (MQ=255)
      ggCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGt                                                                                   <  2:3436434/93‑1 (MQ=255)
       gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTg                                                                                  >  2:7918587/1‑93 (MQ=255)
        cATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCCGGCATGCACCTGCGTGa                                                                                 >  1:4171187/1‑93 (MQ=255)
        cATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGa                                                                                 <  2:96086/93‑1 (MQ=255)
        cATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGa                                                                                 >  2:5002671/1‑93 (MQ=255)
         aTGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCATAATGCAGGCATGCACCTGCGTGaa                                                                                >  1:7721923/1‑93 (MQ=255)
          tGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACTAAGCCCAGCGTCAGCGGCAAAATGCAGGCATCCACCTGCGTGaaa                                                                               >  1:67666/1‑93 (MQ=255)
            gTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAAc                                                                             <  2:9123811/93‑1 (MQ=255)
                aGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCATCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCa                                                                         <  2:4019942/93‑1 (MQ=255)
                aGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCa                                                                         >  2:7145250/1‑93 (MQ=255)
                 gTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCAt                                                                        <  1:5173794/93‑1 (MQ=255)
                  tCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATg                                                                       <  1:8283205/93‑1 (MQ=255)
                  tCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATg                                                                       <  1:1000046/93‑1 (MQ=255)
                  tCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATg                                                                       >  2:3789376/1‑93 (MQ=255)
                   cGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGt                                                                      >  1:8464512/1‑93 (MQ=255)
                    gCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGtt                                                                     >  2:5808711/1‑93 (MQ=255)
                    gCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGtt                                                                     >  2:2249468/1‑93 (MQ=255)
                      tGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGc                                                                   >  1:9058444/1‑93 (MQ=255)
                       gAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGcc                                                                  >  1:5067619/1‑93 (MQ=255)
                        aaTGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCg                                                                 <  2:3509750/93‑1 (MQ=255)
                          tGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGa                                                               <  2:8321997/93‑1 (MQ=255)
                            tttCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAg                                                             >  1:458963/1‑93 (MQ=255)
                             ttCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGc                                                            <  1:3799842/93‑1 (MQ=255)
                              tCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCa                                                           <  2:8517236/93‑1 (MQ=255)
                              tCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCa                                                           <  1:2360345/93‑1 (MQ=255)
                               ccGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCag                                                          <  1:1509724/93‑1 (MQ=255)
                               ccGTCGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCag                                                          <  2:3895993/93‑1 (MQ=255)
                                cGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCaga                                                         <  2:4270570/93‑1 (MQ=255)
                                cGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCaga                                                         <  2:1581079/93‑1 (MQ=255)
                                cGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCaga                                                         <  1:6976364/93‑1 (MQ=255)
                                cGTTGCCTCAGCACTCATTCTTTCGGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAGCAGCATGTTGCCGGAAGCaga                                                         <  2:6842365/93‑1 (MQ=255)
                                 gTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCagag                                                        >  1:3893297/1‑93 (MQ=255)
                                 gTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCagag                                                        <  2:1290024/93‑1 (MQ=255)
                                 gTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCagag                                                        <  1:4431882/93‑1 (MQ=255)
                                   tGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGca                                                      <  2:8376907/93‑1 (MQ=255)
                                    gCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaa                                                     <  1:9306644/93‑1 (MQ=255)
                                       tCAGCCCTCATTCTTTGCGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  <  1:5450419/93‑1 (MQ=255)
                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  1:8616258/1‑93 (MQ=255)
                                        cAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAACCAGCATGTTGCCGGAAGCAGAGCaacaac                                                 <  2:4577167/93‑1 (MQ=255)
                                        cAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCaacaac                                                 >  2:8802800/1‑93 (MQ=255)
                                         aGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACg                                                <  2:2473757/93‑1 (MQ=255)
                                          gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGTCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                               >  2:7750065/1‑93 (MQ=255)
                                           ncnTCATNCTTTACGAAGCCCNGNGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGcc                                              <  1:8800732/92‑1 (MQ=255)
                                             cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                            >  1:1850282/1‑93 (MQ=255)
                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           <  1:8657281/93‑1 (MQ=255)
                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           >  1:5914624/1‑93 (MQ=255)
                                               cATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgtt                                          <  2:666056/93‑1 (MQ=255)
                                                aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGAAACAACGCCtgttg                                         >  1:2635457/1‑93 (MQ=255)
                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                        >  2:3067144/1‑93 (MQ=255)
                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                        >  1:7513256/1‑93 (MQ=255)
                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAAAGCAACAAAGCCtgttgt                                        >  1:2026802/1‑93 (MQ=255)
                                                  tCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTgttgtt                                       <  2:6142922/93‑1 (MQ=255)
                                                  tCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTgttgtt                                       <  2:8009163/93‑1 (MQ=255)
                                                  tCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTgttgtt                                       <  2:9000853/93‑1 (MQ=255)
                                                   cTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGttt                                      <  2:5697949/93‑1 (MQ=255)
                                                    tttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTg                                     <  2:1754168/93‑1 (MQ=255)
                                                     ttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGa                                    >  2:8760261/1‑93 (MQ=255)
                                                      tACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGaa                                   <  2:4952606/93‑1 (MQ=255)
                                                       aCGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAg                                  <  1:3024995/93‑1 (MQ=255)
                                                        cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                 <  2:1968463/93‑1 (MQ=255)
                                                        cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                 >  2:4218459/1‑93 (MQ=255)
                                                         gAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggc                                <  1:7389953/93‑1 (MQ=255)
                                                         gAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggc                                <  1:2801660/93‑1 (MQ=255)
                                                           aGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcgg                              >  1:2053165/1‑93 (MQ=255)
                                                            gCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcggc                             >  2:5307422/1‑93 (MQ=255)
                                                             cccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCt                            <  2:4406758/93‑1 (MQ=255)
                                                              ccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGGTGTTTGAAGGCGGCTa                           >  2:9361168/1‑93 (MQ=255)
                                                               cAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTac                          >  1:9624119/1‑92 (MQ=255)
                                                                aGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATc                         <  2:6727510/93‑1 (MQ=255)
                                                                 gCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATcc                        <  2:2743263/93‑1 (MQ=255)
                                                                  cGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCg                       <  1:6084531/93‑1 (MQ=255)
                                                                   gTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCgg                      >  2:3340307/1‑93 (MQ=255)
                                                                   gTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCgg                      <  2:2985007/93‑1 (MQ=255)
                                                                    tCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGt                     >  1:4820248/1‑93 (MQ=255)
                                                                    tCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGt                     >  1:1030613/1‑93 (MQ=255)
                                                                    tCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGt                     <  1:7891859/93‑1 (MQ=255)
                                                                       gCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCt                  >  2:4302081/1‑93 (MQ=255)
                                                                        cGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTg                 <  2:2817656/93‑1 (MQ=255)
                                                                        cGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTg                 >  2:7616393/1‑93 (MQ=255)
                                                                         ggCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTgg                >  1:9017137/1‑93 (MQ=255)
                                                                          gCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGc               >  1:6203819/1‑93 (MQ=255)
                                                                           cAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGACTGTTGTTTGAAGGGGGATACCCGGggcgggcg              >  1:1260623/1‑93 (MQ=255)
                                                                           cAAAATGCAGGCATGCACCTGCGCGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCg              <  2:5526001/93‑1 (MQ=255)
                                                                            aaaaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGa             >  1:8082470/1‑93 (MQ=255)
                                                                            aaaaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGa             >  2:6598621/1‑93 (MQ=255)
                                                                             aaaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaa            >  2:26052/1‑93 (MQ=255)
                                                                              aaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaaa           <  1:7255114/93‑1 (MQ=255)
                                                                              aaTGAAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaaa           <  2:4650862/93‑1 (MQ=255)
                                                                               aTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAg          <  1:3419315/93‑1 (MQ=255)
                                                                                    ggCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCa     <  1:5718677/93‑1 (MQ=255)
                                                                                     gCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaa    >  2:8239831/1‑93 (MQ=255)
                                                                                      cATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTAGCCGGTGCTGGCGAAAGTCTCaaa   <  2:5146906/93‑1 (MQ=255)
                                                                                       aTGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaaaa  >  1:1145784/1‑93 (MQ=255)
                                                                                       aTGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCaaaa  >  1:9949095/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA  >  NC_000913/3822562‑3822741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 222 total aligned reads displayed.
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGC  >  NC_000913/3822559‑3822744
                                                                                             |                                                                                            
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGC                                                                                              >  SRR4302540.3226531/1‑94 (MQ=60)
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGC                                                                                              >  SRR4302540.7417585/1‑94 (MQ=60)
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGC                                                                                              >  SRR4302540.8445338/1‑94 (MQ=60)
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGC                                                                                              >  SRR4302540.3269491/1‑94 (MQ=60)
  GATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCAC                                                                                            <  SRR4302540.6596213/94‑1 (MQ=60)
  GATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCAC                                                                                            <  SRR4302540.8182836/94‑1 (MQ=60)
   ATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACC                                                                                           <  SRR4302540.3299429/94‑1 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          <  SRR4302540.3374585/94‑1 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          <  SRR4302540.3866261/94‑1 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          >  SRR4302540.733570/1‑94 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          >  SRR4302540.1705883/1‑94 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          >  SRR4302540.4246673/1‑94 (MQ=60)
    TGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCT                                                                                          >  SRR4302540.5417105/1‑94 (MQ=60)
     GGTCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTG                                                                                         <  SRR4302540.1598258/94‑1 (MQ=60)
       TCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCG                                                                                       <  SRR4302540.5786312/94‑1 (MQ=60)
        CGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGT                                                                                      <  SRR4302540.8317830/94‑1 (MQ=60)
        CGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGT                                                                                      <  SRR4302540.6627232/94‑1 (MQ=60)
         GGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTG                                                                                     >  SRR4302540.8018172/1‑94 (MQ=60)
         GGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTG                                                                                     >  SRR4302540.4207867/1‑94 (MQ=60)
          GCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGA                                                                                    <  SRR4302540.5581763/94‑1 (MQ=60)
          GCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGA                                                                                    <  SRR4302540.1043935/94‑1 (MQ=60)
          GCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGA                                                                                    <  SRR4302540.1043933/94‑1 (MQ=60)
           CATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCATAATGCAGGCATGCACCTGCGTGAA                                                                                   >  SRR4302540.7811620/1‑94 (MQ=60)
           CATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAA                                                                                   <  SRR4302540.4274817/94‑1 (MQ=60)
            ATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACTAAGCCCAGCGTCAGCGGCAAAATGCAGGCATCCACCTGCGTGAAA                                                                                  >  SRR4302540.68350/1‑94 (MQ=60)
            ATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAA                                                                                  >  SRR4302540.5154844/1‑94 (MQ=60)
            ATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAA                                                                                  >  SRR4302540.825336/1‑94 (MQ=60)
              GGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAAC                                                                                <  SRR4302540.591610/94‑1 (MQ=60)
                TGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAG                                                                              <  SRR4302540.2060697/94‑1 (MQ=60)
                 GCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGC                                                                             <  SRR4302540.2535246/94‑1 (MQ=60)
                 GCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGC                                                                             <  SRR4302540.6251232/94‑1 (MQ=60)
                 GCAGGCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCCCCTGCGTGAAAACAGC                                                                             <  SRR4302540.8370733/94‑1 (MQ=60)
                  CAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCA                                                                            <  SRR4302540.4697251/94‑1 (MQ=60)
                  CAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCA                                                                            >  SRR4302540.1257436/1‑94 (MQ=60)
                   AGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCAT                                                                           <  SRR4302540.7262704/94‑1 (MQ=60)
                   AGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCAT                                                                           >  SRR4302540.8880031/1‑94 (MQ=60)
                   AGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCAT                                                                           <  SRR4302540.5495803/94‑1 (MQ=60)
                    GTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATG                                                                          >  SRR4302540.1568368/1‑94 (MQ=60)
                    GTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATG                                                                          <  SRR4302540.5230106/94‑1 (MQ=60)
                    GTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATG                                                                          >  SRR4302540.9199643/1‑94 (MQ=60)
                    GTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGAATG                                                                          >  SRR4302540.7908269/1‑94 (MQ=60)
                     TCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGT                                                                         <  SRR4302540.1010204/94‑1 (MQ=60)
                     TCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGT                                                                         <  SRR4302540.6659523/94‑1 (MQ=60)
                     TCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGT                                                                         <  SRR4302540.8379413/94‑1 (MQ=60)
                     TCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGT                                                                         >  SRR4302540.8562793/1‑94 (MQ=60)
                      CGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTT                                                                        <  SRR4302540.8130277/94‑1 (MQ=60)
                      CGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTT                                                                        >  SRR4302540.3201375/1‑94 (MQ=60)
                      CGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTG                                                                        >  SRR4302540.7811240/1‑94 (MQ=60)
                       GCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTG                                                                       <  SRR4302540.2352902/94‑1 (MQ=60)
                       GCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTG                                                                       >  SRR4302540.5054479/1‑94 (MQ=60)
                        CTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGC                                                                      >  SRR4302540.3992863/1‑94 (MQ=60)
                        CTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGC                                                                      >  SRR4302540.895643/1‑94 (MQ=60)
                        CTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGC                                                                      >  SRR4302540.9167195/1‑94 (MQ=60)
                         TGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCG                                                                     >  SRR4302540.2283597/1‑94 (MQ=60)
                           AATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGG                                                                   >  SRR4302540.6178226/1‑94 (MQ=60)
                           AATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGG                                                                   <  SRR4302540.9550038/94‑1 (MQ=60)
                            ATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGA                                                                  >  SRR4302540.9918275/1‑94 (MQ=60)
                             TGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAA                                                                 <  SRR4302540.1717222/94‑1 (MQ=60)
                             TGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAA                                                                 >  SRR4302540.8363727/1‑94 (MQ=60)
                              GTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAG                                                                <  SRR4302540.9026727/94‑1 (MQ=60)
                              GTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAG                                                                >  SRR4302540.463864/1‑94 (MQ=60)
                                 TCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAG                                                             <  SRR4302540.2385773/94‑1 (MQ=60)
                                 TCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAG                                                             <  SRR4302540.2730157/94‑1 (MQ=60)
                                  CCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGA                                                            <  SRR4302540.1526404/94‑1 (MQ=60)
                                  CCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGA                                                            >  SRR4302540.9701840/1‑94 (MQ=60)
                                  CCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGA                                                            <  SRR4302540.6959995/94‑1 (MQ=60)
                                  CCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGA                                                            >  SRR4302540.4229795/1‑94 (MQ=60)
                                   CGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAG                                                           >  SRR4302540.3935559/1‑94 (MQ=60)
                                   CGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAG                                                           >  SRR4302540.5228952/1‑94 (MQ=60)
                                    GTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGC                                                          >  SRR4302540.9824230/1‑94 (MQ=60)
                                    GTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGC                                                          <  SRR4302540.4479893/94‑1 (MQ=60)
                                     TTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCA                                                         <  SRR4302540.4862727/94‑1 (MQ=60)
                                     TTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCA                                                         <  SRR4302540.2687378/94‑1 (MQ=60)
                                       GCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAAC                                                       >  SRR4302540.6205972/1‑94 (MQ=60)
                                       GCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAAC                                                       <  SRR4302540.2071856/94‑1 (MQ=60)
                                       GCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAAC                                                       <  SRR4302540.9417778/94‑1 (MQ=60)
                                        CCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACA                                                      <  SRR4302540.4261718/94‑1 (MQ=60)
                                         CTCCGCCCTCAATCTTTACGAAGCCCAGCGTCAACGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAA                                                     <  SRR4302540.3587138/94‑1 (MQ=60)
                                         CTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGTAGCAGAGCAACAA                                                     >  SRR4302540.6817902/1‑94 (MQ=60)
                                         CTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAA                                                     >  SRR4302540.8716364/1‑94 (MQ=60)
                                         CTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAA                                                     >  SRR4302540.2713073/1‑94 (MQ=60)
                                         CTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAA                                                     >  SRR4302540.655977/1‑94 (MQ=60)
                                          TCAGCCCTCATTCTTTGCGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAAC                                                    <  SRR4302540.5510399/94‑1 (MQ=60)
                                            AGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGC                                                  >  SRR4302540.5482287/1‑94 (MQ=60)
                                              NCNTCATNCTTTACGAAGCCCNGNGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCT                                                <  SRR4302540.8902479/94‑1 (MQ=60)
                                              CCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCT                                                >  SRR4302540.2982480/1‑94 (MQ=60)
                                              CCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCT                                                <  SRR4302540.5754386/94‑1 (MQ=60)
                                              CCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCT                                                <  SRR4302540.2458064/94‑1 (MQ=60)
                                              CCCTCATTCTGTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCT                                                <  SRR4302540.763302/94‑1 (MQ=60)
                                               CCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTG                                               >  SRR4302540.1870573/1‑94 (MQ=60)
                                               CCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTG                                               >  SRR4302540.3049094/1‑94 (MQ=60)
                                               CCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTG                                               >  SRR4302540.1011598/1‑94 (MQ=60)
                                               CCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTG                                               >  SRR4302540.7146551/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.6943734/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.8693697/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.7421235/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.1662392/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.6038068/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              >  SRR4302540.5979966/1‑94 (MQ=60)
                                                CTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGT                                              <  SRR4302540.5954478/94‑1 (MQ=60)
                                                 TCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTT                                             <  SRR4302540.8757849/94‑1 (MQ=60)
                                                  CATTCTTTACTAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCGTGTTG                                            >  SRR4302540.2460890/1‑94 (MQ=60)
                                                  CATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTG                                            >  SRR4302540.9573026/1‑94 (MQ=60)
                                                   ATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGT                                           <  SRR4302540.8338150/94‑1 (MQ=60)
                                                   ATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGT                                           >  SRR4302540.7597254/1‑94 (MQ=60)
                                                   ATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAAAGCAACAAAGCCTGTTGT                                           >  SRR4302540.2048692/1‑94 (MQ=60)
                                                    TTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTT                                          >  SRR4302540.7340518/1‑94 (MQ=60)
                                                    TTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTT                                          >  SRR4302540.6663710/1‑94 (MQ=60)
                                                    TTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTT                                          <  SRR4302540.5384561/94‑1 (MQ=60)
                                                     TCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTT                                         <  SRR4302540.10087075/94‑1 (MQ=60)
                                                      CTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTG                                        >  SRR4302540.1586875/1‑94 (MQ=60)
                                                      CTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTG                                        <  SRR4302540.7586775/94‑1 (MQ=60)
                                                      CTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTG                                        <  SRR4302540.7586774/94‑1 (MQ=60)
                                                      CTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTG                                        <  SRR4302540.4685769/94‑1 (MQ=60)
                                                      CTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTG                                        >  SRR4302540.8725204/1‑94 (MQ=60)
                                                        TTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAA                                      >  SRR4302540.5216686/1‑94 (MQ=60)
                                                          ACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGG                                    <  SRR4302540.3058224/94‑1 (MQ=60)
                                                          ACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGG                                    <  SRR4302540.8708922/94‑1 (MQ=60)
                                                           CGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGC                                   >  SRR4302540.6986987/1‑94 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCACCAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.1483658/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.2053139/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.3550507/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.2831919/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.1989139/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  >  SRR4302540.6244509/1‑94 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.2831917/94‑1 (MQ=60)
                                                            GAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCG                                  <  SRR4302540.3044438/94‑1 (MQ=60)
                                                             AAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCACCAACGCCTGTTGTTTGAAGGCGG                                 >  SRR4302540.4173452/1‑94 (MQ=60)
                                                             AAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGG                                 >  SRR4302540.9282798/1‑94 (MQ=60)
                                                             AAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGG                                 <  SRR4302540.760757/94‑1 (MQ=60)
                                                             AAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGG                                 >  SRR4302540.2075271/1‑94 (MQ=60)
                                                              AGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGC                                <  SRR4302540.8718512/94‑1 (MQ=60)
                                                                CCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTA                              >  SRR4302540.1308537/1‑94 (MQ=60)
                                                                 CCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTAT                             >  SRR4302540.8820060/1‑94 (MQ=60)
                                                                 CCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTAC                             >  SRR4302540.9737549/1‑94 (MQ=60)
                                                                  CAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATC                            <  SRR4302540.1091772/94‑1 (MQ=60)
                                                                   AGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCC                           >  SRR4302540.1667921/1‑94 (MQ=60)
                                                                   AGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCC                           <  SRR4302540.808800/94‑1 (MQ=60)
                                                                    GCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCG                          <  SRR4302540.7356555/94‑1 (MQ=60)
                                                                    GCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCG                          <  SRR4302540.1170872/94‑1 (MQ=60)
                                                                    GCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCG                          <  SRR4302540.9147450/94‑1 (MQ=60)
                                                                    GCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCG                          <  SRR4302540.9128809/94‑1 (MQ=60)
                                                                     CGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGG                         <  SRR4302540.6152245/94‑1 (MQ=60)
                                                                     CGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGG                         >  SRR4302540.3903172/1‑94 (MQ=60)
                                                                     CGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGG                         <  SRR4302540.5032169/94‑1 (MQ=60)
                                                                     CGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGG                         >  SRR4302540.6540469/1‑94 (MQ=60)
                                                                      tgCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        <  SRR4302540.8595964/92‑1 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        <  SRR4302540.9202300/94‑1 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        <  SRR4302540.124712/94‑1 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.7098995/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.1041080/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.8760497/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.1041081/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.4526875/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.4871847/1‑94 (MQ=60)
                                                                      GTCAGCGGCAAAATGCAGGCATGCACCTGCGGGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGT                        >  SRR4302540.9745488/1‑94 (MQ=60)
                                                                       TCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTG                       <  SRR4302540.7983960/94‑1 (MQ=60)
                                                                         AGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCT                     <  SRR4302540.8076558/94‑1 (MQ=60)
                                                                         AGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCT                     >  SRR4302540.4566344/1‑94 (MQ=60)
                                                                          GCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTG                    >  SRR4302540.7495868/1‑94 (MQ=60)
                                                                          GCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTG                    <  SRR4302540.1962384/94‑1 (MQ=60)
                                                                           CGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGG                   <  SRR4302540.3900141/94‑1 (MQ=60)
                                                                           CGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGG                   >  SRR4302540.9125336/1‑94 (MQ=60)
                                                                           CGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGG                   >  SRR4302540.9125335/1‑94 (MQ=60)
                                                                            GGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGC                  <  SRR4302540.8231841/94‑1 (MQ=60)
                                                                            GGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGC                  >  SRR4302540.2270386/1‑94 (MQ=60)
                                                                            GGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGC                  >  SRR4302540.6272874/1‑94 (MQ=60)
                                                                            GGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGC                  >  SRR4302540.5004644/1‑94 (MQ=60)
                                                                             GCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCG                 <  SRR4302540.8909532/94‑1 (MQ=60)
                                                                             GCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCG                 <  SRR4302540.2047061/94‑1 (MQ=60)
                                                                             GCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCG                 >  SRR4302540.1273555/1‑94 (MQ=60)
                                                                             GCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGACTGTTGTTTGAAGGgggatacccggggcgggcg                 >  SRR4302540.1273554/1‑75 (MQ=60)
                                                                              CAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGA                >  SRR4302540.1822722/1‑94 (MQ=60)
                                                                              CAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGA                >  SRR4302540.1822723/1‑94 (MQ=60)
                                                                              CAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGA                <  SRR4302540.3635372/94‑1 (MQ=60)
                                                                              CAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGA                >  SRR4302540.8176278/1‑94 (MQ=60)
                                                                              CAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGGGCTGGCGA                >  SRR4302540.5814788/1‑94 (MQ=60)
                                                                                 AATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAG             <  SRR4302540.7336072/94‑1 (MQ=60)
                                                                                 AATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAG             <  SRR4302540.8613697/94‑1 (MQ=60)
                                                                                  ATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGT            <  SRR4302540.3456514/94‑1 (MQ=60)
                                                                                   TGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTC           <  SRR4302540.1663160/94‑1 (MQ=60)
                                                                                   TGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTC           <  SRR4302540.8538198/94‑1 (MQ=60)
                                                                                   TGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTC           <  SRR4302540.1015770/94‑1 (MQ=60)
                                                                                   TGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTC           <  SRR4302540.8337565/94‑1 (MQ=60)
                                                                                    GCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCG          >  SRR4302540.1175926/1‑94 (MQ=60)
                                                                                    GCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCG          <  SRR4302540.1569720/94‑1 (MQ=60)
                                                                                       GGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAA       <  SRR4302540.5781620/94‑1 (MQ=60)
                                                                                       GGCAGGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAA       <  SRR4302540.6779223/94‑1 (MQ=60)
                                                                                        GCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAA      >  SRR4302540.6036195/1‑94 (MQ=60)
                                                                                         CATGCACCTGCGTGAAAACAGCATGTTGCCGGCAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGGGCTGGCGAAAGTCGCAAAA     >  SRR4302540.4756612/1‑94 (MQ=60)
                                                                                         CATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA     >  SRR4302540.3868318/1‑94 (MQ=60)
                                                                                         CATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA     >  SRR4302540.848329/1‑94 (MQ=60)
                                                                                         CATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA     <  SRR4302540.7931016/94‑1 (MQ=60)
                                                                                         CATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA     >  SRR4302540.10065258/1‑94 (MQ=60)
                                                                                          ATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAC    >  SRR4302540.4582394/1‑94 (MQ=60)
                                                                                          ATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAC    >  SRR4302540.9331952/1‑94 (MQ=60)
                                                                                          ATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAC    >  SRR4302540.4261280/1‑94 (MQ=60)
                                                                                          ATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAC    >  SRR4302540.3855436/1‑94 (MQ=60)
                                                                                          ATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAAC    <  SRR4302540.7807437/94‑1 (MQ=60)
                                                                                            GCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGC  >  SRR4302540.9639625/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGC  >  NC_000913/3822559‑3822744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑