Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,805	T→C	W222R (TGG→CGG)	spoU →	putative RNA methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,805	0	T	C	99.5%	1213.0 / ‑3.6	377	W222R (TGG→CGG)	spoU	putative RNA methylase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (171/204); total (173/204)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 395 total aligned reads displayed.

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

 aTCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCggg                                                                                        <  1:850389/93‑1 (MQ=255)

  tCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGc                                                                                       <  2:5358261/93‑1 (MQ=255)

   ccGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGct                                                                                      >  1:4119669/1‑93 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   <  2:3234314/93‑1 (MQ=255)

         cTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATg                                                                                >  1:6222047/1‑93 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               >  2:7608438/1‑93 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               >  2:9177446/1‑93 (MQ=255)

           ggCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCa                                                                              <  2:9799710/93‑1 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCt                                                                          >  1:657240/1‑93 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCt                                                                          >  2:7276240/1‑93 (MQ=255)

                aaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTg                                                                         >  2:1660553/1‑93 (MQ=255)

                aaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTg                                                                         >  1:2245488/1‑93 (MQ=255)

                 aGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGc                                                                        <  1:10047550/93‑1 (MQ=255)

                   tCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAg                                                                      <  2:1551278/93‑1 (MQ=255)

                    cGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAgg                                                                     <  1:6985097/93‑1 (MQ=255)

                     gCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAggg                                                                    >  1:341741/1‑93 (MQ=255)

                     gCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAggg                                                                    <  1:5130039/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  2:3436580/93‑1 (MQ=255)

                       aaaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTa                                                                  <  2:839425/93‑1 (MQ=255)

                         aaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAg                                                                >  1:5852745/1‑93 (MQ=255)

                         aaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGAAGGGAAAg                                                                >  2:3176807/1‑93 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGt                                                               >  2:4302491/1‑93 (MQ=255)

                            gCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGc                                                             >  1:3531342/1‑93 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           >  1:450367/1‑93 (MQ=255)

                               aaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAt                                                          <  2:6089691/93‑1 (MQ=255)

                                   cctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaaa                                                      >  2:2036049/1‑93 (MQ=255)

                                   cctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaaa                                                      <  1:3665573/93‑1 (MQ=255)

                                   cctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaaa                                                      <  2:9549813/93‑1 (MQ=255)

                                    ctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAg                                                     <  1:3865032/93‑1 (MQ=255)

                                    ctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAg                                                     <  1:2410271/93‑1 (MQ=255)

                                    ctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAg                                                     >  2:2337998/1‑93 (MQ=255)

                                     tgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAgg                                                    >  2:6524509/1‑93 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGATATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  2:9826623/93‑1 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  2:5732973/93‑1 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  >  1:7668246/1‑93 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  1:8792413/93‑1 (MQ=255)

                                        ctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCg                                                 >  2:2956683/1‑93 (MQ=255)

                                         ttATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGc                                                <  1:3157331/93‑1 (MQ=255)

                                         ttATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGc                                                >  2:118131/1‑93 (MQ=255)

                                         ttATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAAGGTAAGTGCCATGAAAGGTAGc                                                >  1:6770/1‑93 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               <  1:428080/93‑1 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               <  1:7991697/93‑1 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               >  2:4636010/1‑93 (MQ=255)

                                           ttCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGATACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCt                                              <  1:5795074/92‑1 (MQ=255)

                                           aTCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCt                                              <  2:6969692/93‑1 (MQ=255)

                                           aTCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGCCt                                              >  1:2710675/1‑93 (MQ=255)

                                            tCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTg                                             <  1:9002104/93‑1 (MQ=255)

                                            tCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTg                                             <  2:7497742/93‑1 (MQ=255)

                                                cACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCGAGGTAAGTGCCCTGAAAGGTCGCCTGTTAg                                         >  1:9616062/1‑93 (MQ=255)

                                                cACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAg                                         >  1:6431216/1‑93 (MQ=255)

                                                cACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAg                                         >  1:8685937/1‑93 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  1:7265059/93‑1 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  2:8593800/93‑1 (MQ=255)

                                                  cGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAt                                       <  1:9065731/93‑1 (MQ=255)

                                                   gTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATg                                      >  2:7043246/1‑93 (MQ=255)

                                                    tCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGTCATGAAAGGGCGCCTGTTAGATgt                                     >  2:864419/1‑92 (MQ=255)

                                                    tCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGc                                     >  2:2230053/1‑93 (MQ=255)

                                                    tCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGc                                     >  1:9313268/1‑93 (MQ=255)

                                                      aaTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTg                                   >  1:3307129/1‑93 (MQ=255)

                                                       aTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGt                                  <  2:3312884/93‑1 (MQ=255)

                                                        tCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTc                                 >  2:3958530/1‑93 (MQ=255)

                                                         cAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGTTGCTGTcc                                >  2:4532568/1‑93 (MQ=255)

                                                         cAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTcc                                >  2:8074150/1‑93 (MQ=255)

                                                         cAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTcc                                >  1:1871720/1‑93 (MQ=255)

                                                           gCAAGGCGAGTTCGAAGCTGATGCTGACTGGCGGGCTACTATGCAGGCTGCTGGGGAAGTGTCTAGTATGGTCGCTTGTTAGATGCTTTCCCa                              >  2:8933797/1‑93 (MQ=255)

                                                           gCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCa                              >  1:8916211/1‑93 (MQ=255)

                                                            cAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCAc                             <  2:7176837/93‑1 (MQ=255)

                                                             aaGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACt                            >  1:1744763/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           >  2:9378406/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           >  2:4161806/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           >  2:6658988/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           <  1:7370405/93‑1 (MQ=255)

                                                               ggCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCa                          <  2:5067618/93‑1 (MQ=255)

                                                                 cGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGt                        <  2:1295134/93‑1 (MQ=255)

                                                                  gagaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGtt                       >  2:5561959/1‑93 (MQ=255)

                                                                   agaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTc                      >  1:1076133/1‑93 (MQ=255)

                                                                    gaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTcc                     <  2:3826676/93‑1 (MQ=255)

                                                                     aTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTccc                    >  1:6846281/1‑93 (MQ=255)

                                                                      tCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCt                   <  1:520663/93‑1 (MQ=255)

                                                                       cGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTa                  <  2:9099949/93‑1 (MQ=255)

                                                                        gAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTaa                 <  2:4951154/93‑1 (MQ=255)

                                                                         aaGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAc                >  2:588549/1‑93 (MQ=255)

                                                                         aaGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAc                >  1:9466033/1‑93 (MQ=255)

                                                                         aaGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAc                >  1:7807110/1‑93 (MQ=255)

                                                                           gCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACgg              <  2:8348443/93‑1 (MQ=255)

                                                                           gCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACgg              <  1:3067144/93‑1 (MQ=255)

                                                                             tGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGc            >  2:3710123/1‑93 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCg           <  2:2179642/93‑1 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCg           >  2:7865773/1‑93 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCg           <  1:2963019/93‑1 (MQ=255)

                                                                               aTGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGt          >  1:2606518/1‑93 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         <  2:1001424/93‑1 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         <  1:4771880/93‑1 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         >  1:6547488/1‑93 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTg        <  2:2684051/93‑1 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTg        <  2:7721922/93‑1 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTg        <  1:1342908/93‑1 (MQ=255)

                                                                                   cGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgc      <  1:5387051/93‑1 (MQ=255)

                                                                                   cGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgc      <  1:6127194/93‑1 (MQ=255)

                                                                                    gACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgcg     <  2:1803085/93‑1 (MQ=255)

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 202 total aligned reads displayed.

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               >  SRR4302540.9376581/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               >  SRR4302540.8894834/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               <  SRR4302540.6790782/94‑1 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               >  SRR4302540.7057278/1‑94 (MQ=60)

 GCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCG                                                                                              <  SRR4302540.2431613/94‑1 (MQ=60)

 GCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCG                                                                                              <  SRR4302540.4427969/94‑1 (MQ=60)

 GCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCG                                                                                              <  SRR4302540.2695222/94‑1 (MQ=60)

  CTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGG                                                                                             >  SRR4302540.2297584/1‑94 (MQ=60)

   TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGG                                                                                            >  SRR4302540.549368/1‑94 (MQ=60)

   TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGG                                                                                            >  SRR4302540.3854682/1‑94 (MQ=60)

    ATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGC                                                                                           <  SRR4302540.859417/94‑1 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          <  SRR4302540.4676567/94‑1 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          <  SRR4302540.8146916/94‑1 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          >  SRR4302540.4164150/1‑94 (MQ=60)

       CGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTAC                                                                                        <  SRR4302540.9602882/94‑1 (MQ=60)

       CGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTAC                                                                                        >  SRR4302540.6874867/1‑94 (MQ=60)

       CGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTAC                                                                                        <  SRR4302540.275682/94‑1 (MQ=60)

        GGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACT                                                                                       >  SRR4302540.7000175/1‑94 (MQ=60)

        GGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACT                                                                                       >  SRR4302540.7858333/1‑94 (MQ=60)

         GTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTA                                                                                      >  SRR4302540.5628756/1‑94 (MQ=60)

          TGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302540.8185432/94‑1 (MQ=60)

          TGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302540.2292974/94‑1 (MQ=60)

          TGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302540.664000/94‑1 (MQ=60)

          GGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302540.8501757/94‑1 (MQ=60)

          GGCTGGCGAAAGTCCCAAAACGCAAAGGCCTGCCTTATCCCCACGGCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302540.8846775/94‑1 (MQ=60)

           GCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATG                                                                                    <  SRR4302540.5388783/94‑1 (MQ=60)

           GCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATG                                                                                    >  SRR4302540.6291258/1‑94 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  <  SRR4302540.6580572/94‑1 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 >  SRR4302540.7665970/1‑94 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 <  SRR4302540.3202140/94‑1 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 <  SRR4302540.2676402/94‑1 (MQ=60)

               GCGGAAGTCGCAAAACGCAAAGGGCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                <  SRR4302540.3054531/94‑1 (MQ=60)

               GCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                <  SRR4302540.2288799/94‑1 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               <  SRR4302540.6759739/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGTCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              >  SRR4302540.9156579/1‑94 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              >  SRR4302540.664252/1‑94 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302540.8084177/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              >  SRR4302540.3496822/1‑94 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTG                                                                             >  SRR4302540.2269613/1‑94 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTG                                                                             >  SRR4302540.3049158/1‑94 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGATG                                                                             >  SRR4302540.3929839/1‑94 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           <  SRR4302540.10164345/94‑1 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           <  SRR4302540.8037621/94‑1 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           >  SRR4302540.946142/1‑94 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          >  SRR4302540.1022819/1‑94 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          >  SRR4302540.5395464/1‑94 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          >  SRR4302540.73178/1‑94 (MQ=60)

                      TCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGG                                                                         <  SRR4302540.2896784/94‑1 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        <  SRR4302540.7062660/94‑1 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        >  SRR4302540.345202/1‑94 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        <  SRR4302540.7610885/94‑1 (MQ=60)

                        GCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGT                                                                       <  SRR4302540.5185602/94‑1 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      >  SRR4302540.2939449/1‑94 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302540.6765437/94‑1 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     >  SRR4302540.4957802/1‑94 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     <  SRR4302540.8734470/94‑1 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAA                                                                     >  SRR4302540.8740971/1‑94 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302540.9774060/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    >  SRR4302540.5917478/1‑94 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302540.2042979/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302540.3146532/94‑1 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGC                                                                 >  SRR4302540.3569885/1‑94 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGAAAGTGC                                                                 >  SRR4302540.5335854/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               <  SRR4302540.5190036/94‑1 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302540.455164/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302540.6621441/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               <  SRR4302540.9137413/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302540.1365404/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302540.3103444/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302540.8890837/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302540.5876175/94‑1 (MQ=60)

                                 AAAGCACTGCCGTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCGGCAGGGTAAGTGCCAT                                                              <  SRR4302540.1365403/94‑1 (MQ=60)

                                    GGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           <  SRR4302540.270215/94‑1 (MQ=60)

                                      GCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302540.8642080/94‑1 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302540.3705686/94‑1 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302540.2590605/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        >  SRR4302540.6716536/1‑94 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302540.3907045/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302540.2436257/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302540.4540267/94‑1 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       >  SRR4302540.153180/1‑94 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       <  SRR4302540.3301244/94‑1 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       <  SRR4302540.5223005/94‑1 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      <  SRR4302540.6095952/94‑1 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      <  SRR4302540.4497718/94‑1 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      >  SRR4302540.7754659/1‑94 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      >  SRR4302540.3813646/1‑94 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     <  SRR4302540.8894119/94‑1 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     >  SRR4302540.4536158/1‑94 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     <  SRR4302540.9139159/94‑1 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGC                                                    >  SRR4302540.6609817/1‑94 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAAGGTAAGTGCCATGAAAGGTAGC                                                    >  SRR4302540.6845/1‑94 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCC                                                   <  SRR4302540.3191692/94‑1 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCC                                                   <  SRR4302540.2456130/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302540.432582/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  >  SRR4302540.6902848/1‑94 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302540.7769913/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302540.1918449/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302540.8084734/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGCCT                                                  >  SRR4302540.2739905/1‑94 (MQ=60)

                                              TTCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGATACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTG                                                 <  SRR4302540.5859109/94‑1 (MQ=60)

                                              ATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTAGTATGCAGGCCGCAGGGTAAGGGCCATGAAAGGTCGCCTT                                                 >  SRR4302540.6991447/1‑94 (MQ=60)

                                              ATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGACGTGCCATGAAAGGGCGCCTT                                                 >  SRR4302540.3469897/1‑94 (MQ=60)

                                               TCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGT                                                <  SRR4302540.9110107/94‑1 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTT                                               >  SRR4302540.6210710/1‑94 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTT                                               <  SRR4302540.3081218/94‑1 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCGAGGTAAGTGCCCTGAAAGGTCGCCTGTTAG                                             >  SRR4302540.9729431/1‑94 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             >  SRR4302540.6502798/1‑94 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             >  SRR4302540.8786797/1‑94 (MQ=60)

                                                   CACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGA                                            <  SRR4302540.322436/94‑1 (MQ=60)

                                                    CCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.4608476/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAGGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.7702020/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.7224868/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.7110382/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.7432341/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.1242283/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.9362174/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.4264383/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302540.7346125/94‑1 (MQ=60)

                                                     CGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATG                                          <  SRR4302540.9174585/94‑1 (MQ=60)

                                                      GTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGC                                         >  SRR4302540.3828469/1‑94 (MQ=60)

                                                      GTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGC                                         >  SRR4302540.9203779/1‑94 (MQ=60)

                                                      GTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGC                                         >  SRR4302540.9424431/1‑94 (MQ=60)

                                                       TCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCT                                        >  SRR4302540.9460052/1‑94 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGGAGGCTGCAGGGTAAGGGCCATGAAAGGTCGCCGGTTAGATGCTG                                       >  SRR4302540.3343355/1‑94 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTG                                       >  SRR4302540.3343354/1‑94 (MQ=60)

                                                         AATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGTTGCAGGGTCAGCGCCATCAAAGGTCGCCTGTTAGATGCTGT                                      >  SRR4302540.1810549/1‑94 (MQ=60)

                                                         AATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGT                                      >  SRR4302540.9023103/1‑94 (MQ=60)

                                                         AATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGT                                      <  SRR4302540.3373583/94‑1 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302540.8970179/1‑94 (MQ=60)

                                                           TCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCC                                    >  SRR4302540.1892232/1‑94 (MQ=60)

                                                            CAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCC                                   >  SRR4302540.1343648/1‑94 (MQ=60)

                                                             AGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCA                                  <  SRR4302540.8904563/94‑1 (MQ=60)

                                                             AGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCA                                  >  SRR4302540.9019476/1‑94 (MQ=60)

                                                               CAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACT                                >  SRR4302540.1763646/1‑94 (MQ=60)

                                                                 ATGCGAGATCGAAGCTGATGCCGACTGGAGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              <  SRR4302540.7452561/94‑1 (MQ=60)

                                                                 AGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              <  SRR4302540.7452562/94‑1 (MQ=60)

                                                                 AGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              >  SRR4302540.5972172/1‑94 (MQ=60)

                                                                   GCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGT                            <  SRR4302540.3479485/94‑1 (MQ=60)

                                                                   GCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGGCGCCTGTTAGATGCTGTCCCACTCAGT                            >  SRR4302540.3653995/1‑94 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTC                          >  SRR4302540.6516861/1‑94 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTC                          >  SRR4302540.1087130/1‑94 (MQ=60)

                                                                       GATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCC                        >  SRR4302540.6922125/1‑94 (MQ=60)

                                                                        ATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCT                       <  SRR4302540.4280489/94‑1 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      <  SRR4302540.1841308/94‑1 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      >  SRR4302540.5815631/1‑94 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      <  SRR4302540.3689967/94‑1 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      <  SRR4302540.526247/94‑1 (MQ=60)

                                                                          CGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAA                     >  SRR4302540.1957478/1‑94 (MQ=60)

                                                                          CGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAA                     >  SRR4302540.6398804/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    <  SRR4302540.7025025/94‑1 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302540.9578331/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302540.7898342/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302540.6404500/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGATGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    <  SRR4302540.1061271/94‑1 (MQ=60)

                                                                             AGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGG                  >  SRR4302540.736785/1‑94 (MQ=60)

                                                                             AGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGG                  >  SRR4302540.3959378/1‑94 (MQ=60)

                                                                             AGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGG                  <  SRR4302540.3158864/94‑1 (MQ=60)

                                                                             AGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGG                  >  SRR4302540.9723408/1‑94 (MQ=60)

                                                                             AGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGG                  <  SRR4302540.5249773/94‑1 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 >  SRR4302540.8069479/1‑94 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 <  SRR4302540.9505823/94‑1 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 <  SRR4302540.3100794/94‑1 (MQ=60)

                                                                               CTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGC                <  SRR4302540.10073630/94‑1 (MQ=60)

                                                                                TGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCG               >  SRR4302540.1884317/1‑94 (MQ=60)

                                                                                 GATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGT              >  SRR4302540.2634651/1‑94 (MQ=60)

                                                                                 GATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGT              <  SRR4302540.2995700/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTGCTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             <  SRR4302540.9077299/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             >  SRR4302540.6227412/1‑94 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             >  SRR4302540.9052091/1‑94 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             >  SRR4302540.5957089/1‑94 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             <  SRR4302540.638075/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             <  SRR4302540.1559145/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTT             >  SRR4302540.6620118/1‑94 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGAGTT             >  SRR4302540.6620120/1‑94 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAAGGGCGTT             <  SRR4302540.3993639/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGATGTCCCACTCAGTTCCCTAACGGGCGTT             >  SRR4302540.6227414/1‑94 (MQ=60)

                                                                                   TTCCGGCTGGTGTGCTACTATGCAGGCTGCAGGGTAAGTGGCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302540.6885846/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302540.2641486/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302540.896646/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302540.4822858/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302540.4822857/94‑1 (MQ=60)

                                                                                    GCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGG           <  SRR4302540.1356984/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302540.4598872/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302540.7840563/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302540.5872907/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302540.1177458/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302540.9263006/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302540.6195435/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302540.5446301/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302540.2542271/94‑1 (MQ=60)

                                                                                       GACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCCGTTCCCTAACGGGCGTTGGCGC        >  SRR4302540.3602715/1‑94 (MQ=60)

                                                                                       GACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGC        <  SRR4302540.4302740/94‑1 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      >  SRR4302540.9626673/1‑94 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      >  SRR4302540.5864268/1‑94 (MQ=60)

                                                                                           GGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCA    <  SRR4302540.6181036/94‑1 (MQ=60)

                                                                                           GGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCA    <  SRR4302540.2305527/94‑1 (MQ=60)

                                                                                           GGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCA    >  SRR4302540.2820737/1‑94 (MQ=60)

                                                                                             CGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  SRR4302540.2555753/1‑94 (MQ=60)

                                                                                             CGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  SRR4302540.3480724/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`