Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,805	T→C	W222R (TGG→CGG)	spoU →	putative RNA methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,805	0	T	C	100.0%	837.8 / NA	257	W222R (TGG→CGG)	spoU	putative RNA methylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (135/122); total (135/122)

Only 100 of 267 total aligned reads displayed.

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

 aTCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCggg                                                                                        <  2:1716232/93‑1 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   <  2:931820/93‑1 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   >  2:5726801/1‑93 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   >  1:3402479/1‑93 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   >  2:4204607/1‑93 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   <  1:2392468/93‑1 (MQ=255)

       tGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctacta                                                                                  <  1:2161205/93‑1 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               <  2:5113998/93‑1 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               <  1:3815855/93‑1 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               >  2:2555118/1‑93 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               <  1:6273269/93‑1 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               <  1:4091063/93‑1 (MQ=255)

           ggCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCa                                                                              >  1:1195795/1‑93 (MQ=255)

           ggCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCa                                                                              <  1:5809693/93‑1 (MQ=255)

              gAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGc                                                                           <  1:305468/93‑1 (MQ=255)

              gAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCACGc                                                                           >  2:3311352/1‑93 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGGAGGCt                                                                          >  2:4785614/1‑93 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCt                                                                          >  2:2237445/1‑93 (MQ=255)

                aaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTg                                                                         >  1:997000/1‑93 (MQ=255)

                  gTCGCAAAACGCAAGGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAAGCTGCa                                                                       >  1:2132007/1‑93 (MQ=255)

                  gTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCa                                                                       <  1:2755975/93‑1 (MQ=255)

                  gTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCa                                                                       <  1:1822768/93‑1 (MQ=255)

                     gCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAggg                                                                    <  2:6153464/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  2:6134475/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  1:4425613/93‑1 (MQ=255)

                       aaaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTa                                                                  <  2:3230616/93‑1 (MQ=255)

                       aaaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTa                                                                  <  1:1365376/93‑1 (MQ=255)

                        aaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTaa                                                                 <  1:2209918/93‑1 (MQ=255)

                        aaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTaa                                                                 <  1:4140017/93‑1 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTTGTATGCAGGCTGCAGGGTAAgc                                                               >  1:2262357/1‑92 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGt                                                               <  2:6018562/93‑1 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGATACTATGCAGGATTCAGGATAAga                                                               >  1:109361/1‑92 (MQ=255)

                           cGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTg                                                              <  1:3224909/93‑1 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           >  2:5879980/1‑93 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           >  1:5164767/1‑93 (MQ=255)

                                   cctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaaa                                                      <  1:4809436/93‑1 (MQ=255)

                                    ctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAg                                                     <  1:276806/93‑1 (MQ=255)

                                    ctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAg                                                     >  1:5707230/1‑93 (MQ=255)

                                     tgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAgg                                                    <  2:4365552/93‑1 (MQ=255)

                                     tgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCATGAAAgg                                                    >  2:4555067/1‑93 (MQ=255)

                                      gcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTGAGCGGCATGAAAGGt                                                   >  2:3424705/1‑93 (MQ=255)

                                      gcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGt                                                   <  2:5362453/93‑1 (MQ=255)

                                      gcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGt                                                   >  1:2330049/1‑93 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  2:3061801/93‑1 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  1:3547764/93‑1 (MQ=255)

                                        ctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCg                                                 >  2:4761818/1‑93 (MQ=255)

                                        ctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCg                                                 >  1:611057/1‑93 (MQ=255)

                                         ttATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGc                                                >  2:3871553/1‑93 (MQ=255)

                                          tagccccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               <  2:2388130/90‑1 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               >  1:2901794/1‑93 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               >  2:1526138/1‑93 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               <  1:5537207/93‑1 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               >  1:6059156/1‑93 (MQ=255)

                                            tCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTg                                             <  2:3668971/93‑1 (MQ=255)

                                              cccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGGCTGtt                                           >  1:4266210/1‑93 (MQ=255)

                                              cccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGtt                                           >  1:1524188/1‑93 (MQ=255)

                                              cccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGtt                                           >  2:3606375/1‑93 (MQ=255)

                                               ccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTa                                          <  2:693295/93‑1 (MQ=255)

                                               ccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTa                                          >  1:2511871/1‑93 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  2:53018/93‑1 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  1:5268437/93‑1 (MQ=255)

                                                  cGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAt                                       >  1:4625610/1‑93 (MQ=255)

                                                  cGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAt                                       <  1:682042/93‑1 (MQ=255)

                                                   gTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATg                                      >  2:4987676/1‑93 (MQ=255)

                                                    tCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGGCGCCTGTTAGATGc                                     >  2:3923071/1‑93 (MQ=255)

                                                        tCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTc                                 <  2:3947163/93‑1 (MQ=255)

                                                            cAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCAc                             <  1:2679906/93‑1 (MQ=255)

                                                             aaGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACt                            <  1:4050704/93‑1 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           >  2:3016231/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTc                           >  2:484588/1‑93 (MQ=255)

                                                               ggCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTGAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCa                          >  2:6011336/1‑93 (MQ=255)

                                                               ggCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCa                          <  2:4096551/93‑1 (MQ=255)

                                                                  gagaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGtt                       >  1:5728895/1‑93 (MQ=255)

                                                                  gagaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTCTCCCACTCAGtt                       >  2:2066799/1‑93 (MQ=255)

                                                                     aTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTccc                    <  2:1331070/93‑1 (MQ=255)

                                                                      tCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCt                   <  1:1009420/93‑1 (MQ=255)

                                                                      tCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCt                   >  1:1598305/1‑93 (MQ=255)

                                                                       cGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTa                  <  2:5382203/93‑1 (MQ=255)

                                                                       cGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCACTa                  >  2:5750092/1‑93 (MQ=255)

                                                                          aGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACg               >  2:3138374/1‑93 (MQ=255)

                                                                          aGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACg               >  2:183385/1‑93 (MQ=255)

                                                                           gCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACgg              <  1:4200543/93‑1 (MQ=255)

                                                                           gCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACgg              <  1:799912/93‑1 (MQ=255)

                                                                           gCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACgg              >  2:2199944/1‑93 (MQ=255)

                                                                            cTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACggg             <  2:1184162/93‑1 (MQ=255)

                                                                            cTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACggg             >  2:1956867/1‑93 (MQ=255)

                                                                            cTGATGCCGACTGGCGGGCTACTATGCACGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACggg             >  1:4952213/1‑93 (MQ=255)

                                                                             tGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGc            >  1:2025422/1‑93 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCCCTCAGTTCCCTAACGGGCg           >  1:3584461/1‑93 (MQ=255)

                                                                               aTGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGCAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTTCCTAACCGGCGt          >  2:2488175/1‑93 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGGCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         <  2:2777641/93‑1 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         <  1:1995479/93‑1 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTg        >  1:4763826/1‑93 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTg        >  1:1803538/1‑93 (MQ=255)

                                                                                 gCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAAGTCGCCTGTTAGATGCTGTCCCACTCTGTTCCCTAACGGGCGGTg        >  2:5581341/1‑93 (MQ=255)

                                                                                    gACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgcg     >  1:543738/1‑93 (MQ=255)

                                                                                     aCTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgcgc    >  2:1663051/1‑93 (MQ=255)

                                                                                     aCTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgcgc    <  1:5014938/93‑1 (MQ=255)

                                                                                       tggcggGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCgcag  <  2:1155447/93‑1 (MQ=255)

                                                                                       tggcggGCAACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCgcag  >  2:6115355/1‑93 (MQ=255)

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGG                                                                                               >  SRR4302533.734561/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               <  SRR4302533.2449691/94‑1 (MQ=60)

  CTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGG                                                                                             <  SRR4302533.4831859/94‑1 (MQ=60)

  CTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGG                                                                                             <  SRR4302533.5107081/94‑1 (MQ=60)

   TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGG                                                                                            >  SRR4302533.2390130/1‑94 (MQ=60)

    ATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGC                                                                                           >  SRR4302533.3823951/1‑94 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          >  SRR4302533.5181337/1‑94 (MQ=60)

      CCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTA                                                                                         >  SRR4302533.5870747/1‑94 (MQ=60)

       CGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTAC                                                                                        >  SRR4302533.2334925/1‑94 (MQ=60)

        GGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACT                                                                                       >  SRR4302533.3423488/1‑94 (MQ=60)

         GTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTA                                                                                      <  SRR4302533.2407236/94‑1 (MQ=60)

          TGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAT                                                                                     <  SRR4302533.2174507/94‑1 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  <  SRR4302533.3839187/94‑1 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  <  SRR4302533.4115967/94‑1 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  <  SRR4302533.6310739/94‑1 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  >  SRR4302533.1202982/1‑94 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 <  SRR4302533.5844777/94‑1 (MQ=60)

               GCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                >  SRR4302533.3606436/1‑94 (MQ=60)

               GCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                >  SRR4302533.4109218/1‑94 (MQ=60)

               GCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                >  SRR4302533.5807548/1‑94 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               >  SRR4302533.1830897/1‑94 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302533.307335/94‑1 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTG                                                                             >  SRR4302533.1002821/1‑94 (MQ=60)

                    AGTCGCAAAACGCAAGGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAAGCTGCA                                                                           >  SRR4302533.2145155/1‑94 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           <  SRR4302533.4934664/94‑1 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          <  SRR4302533.2996090/94‑1 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          <  SRR4302533.1833902/94‑1 (MQ=60)

                     GTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          <  SRR4302533.2772877/94‑1 (MQ=60)

                      TCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGG                                                                         <  SRR4302533.2148173/94‑1 (MQ=60)

                      TCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAAG                                                                         >  SRR4302533.4498906/1‑94 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        >  SRR4302533.4967564/1‑94 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        >  SRR4302533.5659350/1‑94 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302533.921983/94‑1 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      >  SRR4302533.129797/1‑94 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302533.4452364/94‑1 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     <  SRR4302533.2069223/94‑1 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     <  SRR4302533.1373586/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGGAAG                                                                    >  SRR4302533.5567295/1‑94 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302533.4165127/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302533.1594677/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302533.2223549/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302533.1595541/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCACACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302533.4214453/94‑1 (MQ=60)

                            AACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTTGTATGCAGGCTGCAGGGTAAGC                                                                   >  SRR4302533.2276351/1‑94 (MQ=60)

                            AACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGATACTATGCAGGattcaggataaga                                                                   >  SRR4302533.110056/1‑81 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGC                                                                 <  SRR4302533.3244792/94‑1 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               <  SRR4302533.502254/94‑1 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302533.5195956/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302533.4497939/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302533.73012/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302533.3362364/1‑94 (MQ=60)

                                  AAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATG                                                             <  SRR4302533.4224309/94‑1 (MQ=60)

                                  AAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATG                                                             >  SRR4302533.4775302/1‑94 (MQ=60)

                                    GGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           >  SRR4302533.3930672/1‑94 (MQ=60)

                                    GGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           <  SRR4302533.2734181/94‑1 (MQ=60)

                                     GCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAA                                                          >  SRR4302533.2924514/1‑94 (MQ=60)

                                     GCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAA                                                          <  SRR4302533.6123245/94‑1 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         >  SRR4302533.5741703/1‑94 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302533.1782289/94‑1 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302533.1851910/94‑1 (MQ=60)

                                      CCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAG                                                         <  SRR4302533.4838587/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302533.5485716/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302533.278493/94‑1 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTCCTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       >  SRR4302533.5268984/1‑94 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       >  SRR4302533.2344474/1‑94 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      <  SRR4302533.2339150/94‑1 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     >  SRR4302533.614650/1‑94 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     <  SRR4302533.3569629/94‑1 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGC                                                    >  SRR4302533.2165109/1‑94 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCC                                                   >  SRR4302533.2919620/1‑94 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCC                                                   >  SRR4302533.6095838/1‑94 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCC                                                   <  SRR4302533.4121261/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302533.5285527/94‑1 (MQ=60)

                                             TATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCT                                                  <  SRR4302533.5570646/94‑1 (MQ=60)

                                              ATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTG                                                 >  SRR4302533.1732886/1‑94 (MQ=60)

                                              ATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGATCGCCTG                                                 <  SRR4302533.2780799/94‑1 (MQ=60)

                                               TCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGT                                                >  SRR4302533.1148119/1‑94 (MQ=60)

                                               TCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGATACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGCCTGT                                                >  SRR4302533.3635402/1‑94 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGGCTGTT                                               >  SRR4302533.4291901/1‑94 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTT                                               >  SRR4302533.1533364/1‑94 (MQ=60)

                                                 CCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTA                                              >  SRR4302533.4793288/1‑94 (MQ=60)

                                                 CCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTA                                              >  SRR4302533.2527348/1‑94 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             >  SRR4302533.2515847/1‑94 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             >  SRR4302533.4445359/1‑94 (MQ=60)

                                                   CACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGA                                            >  SRR4302533.2198814/1‑94 (MQ=60)

                                                   CACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGA                                            <  SRR4302533.1328178/94‑1 (MQ=60)

                                                    CCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302533.427472/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302533.5300040/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           >  SRR4302533.4653646/1‑94 (MQ=60)

                                                     CGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATG                                          <  SRR4302533.686075/94‑1 (MQ=60)

                                                         AATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGT                                      >  SRR4302533.5139218/1‑94 (MQ=60)

                                                         AATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGACGCCTGTTAGATGCTGT                                      >  SRR4302533.2887433/1‑94 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302533.5766291/1‑94 (MQ=60)

                                                           TCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCC                                    <  SRR4302533.2672691/94‑1 (MQ=60)

                                                             AGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCA                                  >  SRR4302533.2010643/1‑94 (MQ=60)

                                                               CAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACT                                <  SRR4302533.2696331/94‑1 (MQ=60)

                                                                AAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTC                               <  SRR4302533.2212248/94‑1 (MQ=60)

                                                                AAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTC                               <  SRR4302533.4075380/94‑1 (MQ=60)

                                                                AAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTC                               <  SRR4302533.4809642/94‑1 (MQ=60)

                                                                 AGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              >  SRR4302533.951955/1‑94 (MQ=60)

                                                                  GGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGCTGCTGTCCCACTCAG                             >  SRR4302533.6272251/1‑94 (MQ=60)

                                                                  GGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAG                             >  SRR4302533.6070238/1‑94 (MQ=60)

                                                                   GCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGT                            <  SRR4302533.3199797/94‑1 (MQ=60)

                                                                    CGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTT                           <  SRR4302533.6284220/94‑1 (MQ=60)

                                                                    CGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTT                           <  SRR4302533.2252094/94‑1 (MQ=60)

                                                                    CGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTT                           >  SRR4302533.5763490/1‑94 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGCAAGGGCGCCTGTTAGATGCTGTCCCACTCAGTTC                          >  SRR4302533.1998565/1‑94 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTGGCCTGTTAGATGCTGTCCCACTCAGGTC                          >  SRR4302533.5575165/1‑94 (MQ=60)

                                                                      AGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCC                         >  SRR4302533.2364815/1‑94 (MQ=60)

                                                                      AGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCC                         <  SRR4302533.2739293/94‑1 (MQ=60)

                                                                        ATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCT                       <  SRR4302533.1021687/94‑1 (MQ=60)

                                                                        ATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCT                       >  SRR4302533.1607981/1‑94 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      <  SRR4302533.1015325/94‑1 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      >  SRR4302533.3849263/1‑94 (MQ=60)

                                                                          CGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAA                     >  SRR4302533.412887/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCTCTCAGCTCCCTAAC                    >  SRR4302533.2668589/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    <  SRR4302533.5755370/94‑1 (MQ=60)

                                                                            AAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACG                   >  SRR4302533.5313280/1‑94 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 <  SRR4302533.804617/94‑1 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 >  SRR4302533.2020706/1‑94 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 <  SRR4302533.4225962/94‑1 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCACGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 >  SRR4302533.4982204/1‑94 (MQ=60)

                                                                               CTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGC                >  SRR4302533.1399280/1‑94 (MQ=60)

                                                                               CTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGC                >  SRR4302533.2037926/1‑94 (MQ=60)

                                                                                TGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCCCTCAGTTCCCTAACGGGCG               >  SRR4302533.3606535/1‑94 (MQ=60)

                                                                                TGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTAGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCG               >  SRR4302533.1128232/1‑94 (MQ=60)

                                                                                 GATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGT              <  SRR4302533.637320/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            >  SRR4302533.4792718/1‑94 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            >  SRR4302533.1814515/1‑94 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302533.2007730/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            >  SRR4302533.3507541/1‑94 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302533.4409013/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302533.663204/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGC          <  SRR4302533.3292182/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302533.2846316/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         >  SRR4302533.546968/1‑94 (MQ=60)

                                                                                        ACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCA       <  SRR4302533.5045322/94‑1 (MQ=60)

                                                                                        ACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCA       >  SRR4302533.3424014/1‑94 (MQ=60)

                                                                                           GGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCA    <  SRR4302533.2524783/94‑1 (MQ=60)

                                                                                             CGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  SRR4302533.4453461/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`