Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,847,265 A→G W167R (TGG→CGG)  b3668 ← sensor histidine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,847,2650AG99.4% 1035.6 / ‑3.3 328W167R (TGG→CGG) b3668sensor histidine protein kinase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (144/181);  total (146/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 363 total aligned reads displayed.

GACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACG  >  NC_000913/3847175‑3847354
                                                                                          |                                                                                         
gaCTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCggg                                                                                         <  2:7343865/93‑1 (MQ=255)
gaCTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCggg                                                                                         <  2:1089353/93‑1 (MQ=255)
gaCTCATAACAAACAGAAGCAAGAACCAGACCAGATGTCGCCCGCGCCAGTGGATTGGCTGAGAACCCAGTGACGGACCGAGCGGCAGCCggg                                                                                         <  2:5855880/93‑1 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      <  1:5101084/93‑1 (MQ=255)
    gATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGtt                                                                                     <  2:9421764/93‑1 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    <  1:1042368/93‑1 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    >  2:489809/1‑93 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    <  1:3637677/93‑1 (MQ=255)
      tAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTAt                                                                                   >  1:3077500/1‑93 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCCGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  >  1:2408560/1‑93 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  <  2:3089220/93‑1 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  <  1:7598779/93‑1 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTAtt                                                                                  >  2:671919/1‑93 (MQ=255)
        acaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTg                                                                                 >  1:7189652/1‑93 (MQ=255)
         caaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTgg                                                                                <  2:569900/93‑1 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCg                                                                              <  2:1583359/93‑1 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCg                                                                              >  1:3094771/1‑93 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACAGAGCGGCAGCCCGGGGTTATTGGCg                                                                              >  1:2491059/1‑93 (MQ=255)
            acaGCAGCAAGTCCCAGACCAGATGTCGCCCGCGCCAGTTGATTGGGTGAGAAGCCAGTGACGGACCGAGCGGCAGCCGGGTGTTAGTGGCga                                                                             <  2:8127598/93‑1 (MQ=255)
            acaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCga                                                                             <  2:3813486/93‑1 (MQ=255)
            acaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCga                                                                             <  2:2919454/93‑1 (MQ=255)
             cagcagCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCgag                                                                            >  2:9094616/1‑93 (MQ=255)
               gcagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAt                                                                          <  1:8540798/93‑1 (MQ=255)
               gcagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAt                                                                          <  2:2601768/93‑1 (MQ=255)
                cagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATa                                                                         <  2:1223677/93‑1 (MQ=255)
                cagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATa                                                                         <  1:4695349/93‑1 (MQ=255)
                 agcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAg                                                                        <  2:4538191/93‑1 (MQ=255)
                  gcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGt                                                                       >  1:6593982/1‑93 (MQ=255)
                   gaaGTACCAAACCAGATGTCGCCCGCGCCAGGTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTg                                                                      <  1:6923780/92‑1 (MQ=255)
                   caAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTg                                                                      >  2:3777102/1‑93 (MQ=255)
                    aaGTACTAGACCAGATGTCGCCTGCGCCAGTTGATTTGCTGAGAAACCAGTGACGGACCGAGCTTCAGTCGGGCGTTATTGACGAGAGTGTGc                                                                     >  2:9250206/1‑93 (MQ=255)
                      gTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCa                                                                   <  1:396941/93‑1 (MQ=255)
                         ccagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGaa                                                                <  2:1778907/93‑1 (MQ=255)
                         ccagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGaa                                                                <  2:4762542/93‑1 (MQ=255)
                         ccagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCCGGTGTTATTGGCGAGATAGTGCCAGaa                                                                >  1:8846999/1‑93 (MQ=255)
                          cagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTTACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAac                                                               >  1:4420896/1‑93 (MQ=255)
                          cagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAac                                                               >  2:4402644/1‑93 (MQ=255)
                          cagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAac                                                               >  1:1003750/1‑93 (MQ=255)
                           agaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAaca                                                              >  1:7875637/1‑93 (MQ=255)
                            gaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAacac                                                             >  1:5938419/1‑93 (MQ=255)
                            gaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAacac                                                             >  1:6769050/1‑93 (MQ=255)
                              ccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCa                                                           <  2:7816181/93‑1 (MQ=255)
                               cagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAg                                                          >  1:1554414/1‑93 (MQ=255)
                                agaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGa                                                         <  2:5086037/93‑1 (MQ=255)
                                agaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGa                                                         >  1:144840/1‑93 (MQ=255)
                                 gaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCACAACACCAGAc                                                        >  2:5612544/1‑93 (MQ=255)
                                  aTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACa                                                       >  2:3228574/1‑93 (MQ=255)
                                  aTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACa                                                       <  2:6126266/93‑1 (MQ=255)
                                  aTGTCGCCCGCGCCAGTTGATTGGCGGAGAACCCAGTGCCGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACa                                                       <  1:2140780/93‑1 (MQ=255)
                                   tGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  <  2:7179614/65‑1 (MQ=255)
                                     tCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACatat                                                    <  2:7721998/93‑1 (MQ=255)
                                      cGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATc                                                   <  2:8671467/93‑1 (MQ=255)
                                         ccGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCggg                                                >  1:9426787/1‑93 (MQ=255)
                                           gcgcCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGc                                              <  1:9163632/93‑1 (MQ=255)
                                            cgcCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGcc                                             <  2:8607621/93‑1 (MQ=255)
                                             gcCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCa                                            >  2:8514284/1‑93 (MQ=255)
                                               cAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGc                                          >  1:7440396/1‑93 (MQ=255)
                                                aGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCg                                         >  1:8380070/1‑93 (MQ=255)
                                                aGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCg                                         >  2:5321239/1‑93 (MQ=255)
                                                 gTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGt                                        >  1:3458374/1‑93 (MQ=255)
                                                  ttGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTc                                       >  2:6953405/1‑93 (MQ=255)
                                                  ttGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTc                                       <  1:6397785/93‑1 (MQ=255)
                                                   tGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCa                                      <  1:7098817/93‑1 (MQ=255)
                                                   tGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCa                                      <  2:6540336/93‑1 (MQ=255)
                                                    gATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCACGCGGCAg                                     >  2:4044095/1‑93 (MQ=255)
                                                     aTTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAgg                                    >  2:101822/1‑93 (MQ=255)
                                                      ttGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGgc                                   >  2:1348610/1‑93 (MQ=255)
                                                      ttGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGgc                                   >  2:8756105/1‑93 (MQ=255)
                                                         gCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGggcgc                                >  2:2459923/1‑93 (MQ=255)
                                                           tGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCa                              <  2:5034211/93‑1 (MQ=255)
                                                           tGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGTTAGTGCCAGAACACCCGACATATCGGGGCCAGCGTCAGGCCGCCa                              >  1:2316349/1‑93 (MQ=255)
                                                           tGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCa                              >  2:9684114/1‑93 (MQ=255)
                                                            gagaAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAg                             <  1:5213791/93‑1 (MQ=255)
                                                              gaAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGtt                           >  2:9455321/1‑93 (MQ=255)
                                                              aaaaCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGtt                           <  1:6173677/92‑1 (MQ=255)
                                                               aacccAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCGAGCGTCAGGCCGCCAGTTa                          <  2:3361337/90‑1 (MQ=255)
                                                                aaCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTaa                         <  1:9665926/93‑1 (MQ=255)
                                                                aaCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTaa                         >  2:1854669/1‑93 (MQ=255)
                                                                 aCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTaaa                        >  2:9534820/1‑93 (MQ=255)
                                                                    aGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTc                     >  2:2689043/1‑93 (MQ=255)
                                                                     gTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAATTCa                    >  1:2094882/1‑93 (MQ=255)
                                                                     gTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCa                    <  2:7044832/93‑1 (MQ=255)
                                                                     gTGACGGACCGAGCGGCAGCCGGGAGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCa                    >  1:3583434/1‑93 (MQ=255)
                                                                       gACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGc                  <  2:5649469/93‑1 (MQ=255)
                                                                        aCGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCa                 <  1:3693279/93‑1 (MQ=255)
                                                                        aCGGACCGAGCGACAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCa                 <  1:7986149/93‑1 (MQ=255)
                                                                         cGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCaa                <  1:8306404/93‑1 (MQ=255)
                                                                           gACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACa              >  2:2053677/1‑93 (MQ=255)
                                                                           gACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACa              >  1:1877739/1‑93 (MQ=255)
                                                                           gACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACa              >  1:9847745/1‑93 (MQ=255)
                                                                            aCCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAg             >  1:6715494/1‑93 (MQ=255)
                                                                             ccGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAgc            <  2:3591428/93‑1 (MQ=255)
                                                                              cGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAgcg           >  2:6376067/1‑93 (MQ=255)
                                                                                aGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCa         <  2:8463220/93‑1 (MQ=255)
                                                                                aGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCa         >  2:1397625/1‑93 (MQ=255)
                                                                                 gCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAt        >  1:4521915/1‑93 (MQ=255)
                                                                                  cGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAtt       <  2:2199420/93‑1 (MQ=255)
                                                                                   ggCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTc      <  1:5489115/93‑1 (MQ=255)
                                                                                   ggCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTc      >  1:4177591/1‑93 (MQ=255)
                                                                                    gCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTcc     <  2:6296753/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACG  >  NC_000913/3847175‑3847354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  NC_000913/3847172‑3847358
                                                                                             |                                                                                             
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.6759513/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.4854624/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.4854625/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.6642337/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.112489/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.5477651/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.5855689/1‑94 (MQ=60)
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               >  SRR4302540.5855690/1‑94 (MQ=60)
 GAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGG                                                                                              <  SRR4302540.1796101/94‑1 (MQ=60)
 GAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCCG                                                                                              >  SRR4302540.1094889/1‑94 (MQ=60)
    ACTGATAACAAACAGCAGCAATAACCAGCCCAGATGTCGCCCGCGCCAGTTGATTGTCTGAGAAGCCAGTGACGGACCGAGCGGCAGCCGGGTG                                                                                           <  SRR4302540.37220/94‑1 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          >  SRR4302540.5782787/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          >  SRR4302540.6441860/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          <  SRR4302540.4533721/94‑1 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         >  SRR4302540.1894206/1‑94 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         <  SRR4302540.5156294/94‑1 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         >  SRR4302540.5118484/1‑94 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         >  SRR4302540.9554150/1‑94 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTT                                                                                         >  SRR4302540.3913325/1‑94 (MQ=60)
       GATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTA                                                                                        >  SRR4302540.7830892/1‑94 (MQ=60)
        ATAACAAACAGCAGCAAGTGCCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302540.385957/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302540.3677504/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302540.1052976/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       >  SRR4302540.5215192/1‑94 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302540.1052964/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       >  SRR4302540.9475807/1‑94 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTAT                                                                                       >  SRR4302540.3111263/1‑94 (MQ=60)
         TAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCCGTGACGGACCGAGCGGCAGCCGGGTGTTATT                                                                                      >  SRR4302540.2434528/1‑94 (MQ=60)
         TAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATT                                                                                      >  SRR4302540.3399090/1‑94 (MQ=60)
         TAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATT                                                                                      >  SRR4302540.7610484/1‑94 (MQ=60)
          AACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTG                                                                                     >  SRR4302540.7269791/1‑94 (MQ=60)
          AACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTG                                                                                     <  SRR4302540.7684159/94‑1 (MQ=60)
          AACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTG                                                                                     >  SRR4302540.6837707/1‑94 (MQ=60)
           ACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGG                                                                                    >  SRR4302540.2057222/1‑94 (MQ=60)
            CAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGC                                                                                   <  SRR4302540.1225048/94‑1 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCG                                                                                  <  SRR4302540.7315824/94‑1 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCG                                                                                  >  SRR4302540.3128700/1‑94 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCG                                                                                  >  SRR4302540.6616639/1‑94 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACAGAGCGGCAGCCCGGGGTTATTGGCG                                                                                  >  SRR4302540.2517886/1‑94 (MQ=60)
                CAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGATAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGA                                                                               >  SRR4302540.7776210/1‑94 (MQ=60)
                CAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGA                                                                               <  SRR4302540.7251339/94‑1 (MQ=60)
                 AGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAT                                                                              <  SRR4302540.6312765/94‑1 (MQ=60)
                 AGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAT                                                                              >  SRR4302540.8085536/1‑94 (MQ=60)
                 AGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAT                                                                              >  SRR4302540.3184613/1‑94 (MQ=60)
                  GCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATA                                                                             <  SRR4302540.764758/94‑1 (MQ=60)
                  GCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATA                                                                             <  SRR4302540.8640037/94‑1 (MQ=60)
                   CAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAG                                                                            >  SRR4302540.6320299/1‑94 (MQ=60)
                   CAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAG                                                                            <  SRR4302540.9291404/94‑1 (MQ=60)
                   CAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAG                                                                            <  SRR4302540.4745544/94‑1 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGCAACCAGTGACGGCACGAGAGGCAGCCGGGTGTTATTGGCGAGATAGA                                                                           >  SRR4302540.58610/1‑94 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGT                                                                           >  SRR4302540.6667008/1‑94 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAGAGT                                                                           >  SRR4302540.618933/1‑94 (MQ=60)
                      GAAGTACCAAACCAGATGTCGCCCGCGCCAGGTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGC                                                                         <  SRR4302540.7000832/94‑1 (MQ=60)
                      CAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGC                                                                         <  SRR4302540.2151549/94‑1 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       <  SRR4302540.3122162/94‑1 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       <  SRR4302540.365804/94‑1 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       >  SRR4302540.8448268/1‑94 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       <  SRR4302540.7066330/94‑1 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCAGGGTGTTATTGGCGAGATAGTGCCA                                                                       <  SRR4302540.931768/94‑1 (MQ=60)
                         GTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAG                                                                      <  SRR4302540.401055/94‑1 (MQ=60)
                          TACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGA                                                                     <  SRR4302540.6621186/94‑1 (MQ=60)
                           ACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGCA                                                                    >  SRR4302540.5910709/1‑94 (MQ=60)
                           ACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCCGGTGTTATTGGCGAGATAGTGCCAGAA                                                                    >  SRR4302540.8949346/1‑94 (MQ=60)
                            CCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTTACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAAC                                                                   >  SRR4302540.4468795/1‑94 (MQ=60)
                            CCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAAC                                                                   >  SRR4302540.1013939/1‑94 (MQ=60)
                            CCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAAC                                                                   >  SRR4302540.2027521/1‑94 (MQ=60)
                             CAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACA                                                                  >  SRR4302540.9816744/1‑94 (MQ=60)
                             CAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACA                                                                  >  SRR4302540.7967626/1‑94 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGCCGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGGGCCAGAACAC                                                                 >  SRR4302540.8561490/1‑94 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 >  SRR4302540.6004063/1‑94 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 >  SRR4302540.6843857/1‑94 (MQ=60)
                               GACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACC                                                                <  SRR4302540.8862668/94‑1 (MQ=60)
                                 CCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAG                                                              >  SRR4302540.4405964/1‑94 (MQ=60)
                                 CCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAG                                                              >  SRR4302540.1571516/1‑94 (MQ=60)
                                 CCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAG                                                              <  SRR4302540.3175979/94‑1 (MQ=60)
                                  CAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGA                                                             <  SRR4302540.5544381/94‑1 (MQ=60)
                                  CAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGA                                                             >  SRR4302540.146345/1‑94 (MQ=60)
                                   AGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAC                                                            >  SRR4302540.190255/1‑94 (MQ=60)
                                     GTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACAT                                                          <  SRR4302540.2372074/94‑1 (MQ=60)
                                     ATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGaagcgctagatcggaagagcggttcag                                                          >  SRR4302540.7259657/1‑67 (MQ=60)
                                     ATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACAT                                                          <  SRR4302540.2372075/94‑1 (MQ=60)
                                     ATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGCGCCAGAACACCAGACAT                                                          <  SRR4302540.7239211/94‑1 (MQ=60)
                                     ATGTCGCCCGCGCCAGTTGATTGGCGGAGAACCCAGTGCCGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACAT                                                          <  SRR4302540.2163785/94‑1 (MQ=60)
                                       GTCGCGCGCGCCAGTTGATTGTCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATAT                                                        <  SRR4302540.6940873/94‑1 (MQ=60)
                                       GTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATAT                                                        >  SRR4302540.2286611/1‑94 (MQ=60)
                                       GTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATAT                                                        <  SRR4302540.796291/94‑1 (MQ=60)
                                         CGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302540.9977384/94‑1 (MQ=60)
                                         CGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302540.1489923/94‑1 (MQ=60)
                                          CCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGG                                                     <  SRR4302540.2386097/94‑1 (MQ=60)
                                           CCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGG                                                    >  SRR4302540.9538793/1‑94 (MQ=60)
                                           CCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGG                                                    <  SRR4302540.7667106/94‑1 (MQ=60)
                                           CCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGG                                                    <  SRR4302540.4589771/94‑1 (MQ=60)
                                            CCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGG                                                   <  SRR4302540.4341016/94‑1 (MQ=60)
                                             CGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGC                                                  <  SRR4302540.8151894/94‑1 (MQ=60)
                                             CGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGC                                                  >  SRR4302540.7026059/1‑94 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCC                                                 <  SRR4302540.9273597/94‑1 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCA                                                 <  SRR4302540.3639987/94‑1 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCC                                                 <  SRR4302540.78842/94‑1 (MQ=60)
                                               CGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCA                                                <  SRR4302540.5208558/94‑1 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302540.7150545/1‑94 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               <  SRR4302540.4874085/94‑1 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302540.10155424/1‑94 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               <  SRR4302540.6037371/94‑1 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302540.1350660/1‑94 (MQ=60)
                                                 CCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGC                                              >  SRR4302540.7523298/1‑94 (MQ=60)
                                                 CCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGC                                              >  SRR4302540.7523299/1‑94 (MQ=60)
                                                  CAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCG                                             >  SRR4302540.8477309/1‑94 (MQ=60)
                                                  CAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCG                                             >  SRR4302540.3705256/1‑94 (MQ=60)
                                                  CAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCG                                             <  SRR4302540.1894414/94‑1 (MQ=60)
                                                   AGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGT                                            >  SRR4302540.3496019/1‑94 (MQ=60)
                                                    GTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTC                                           >  SRR4302540.3861442/1‑94 (MQ=60)
                                                    GTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTC                                           >  SRR4302540.6880387/1‑94 (MQ=60)
                                                    GTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTC                                           >  SRR4302540.4105432/1‑94 (MQ=60)
                                                     TTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCA                                          >  SRR4302540.6808073/1‑94 (MQ=60)
                                                     TTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCA                                          <  SRR4302540.6469009/94‑1 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAG                                         <  SRR4302540.4627287/94‑1 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAG                                         >  SRR4302540.8511313/1‑94 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAG                                         >  SRR4302540.8178121/1‑94 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAG                                         <  SRR4302540.7177811/94‑1 (MQ=60)
                                                       GATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGTGATAGTGCCAGAACGCCATACATATCGGGGCCAGCGTCAGG                                        <  SRR4302540.5429826/94‑1 (MQ=60)
                                                       GATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGG                                        <  SRR4302540.5429825/94‑1 (MQ=60)
                                                        ATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCACACATATCGGGGCCAGCGTCACGC                                       >  SRR4302540.4933425/1‑94 (MQ=60)
                                                         TTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCC                                      <  SRR4302540.4351136/94‑1 (MQ=60)
                                                         TTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCC                                      <  SRR4302540.4186644/94‑1 (MQ=60)
                                                             GTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCA                                  <  SRR4302540.8889411/94‑1 (MQ=60)
                                                             CTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCA                                  >  SRR4302540.8615170/1‑94 (MQ=60)
                                                             CTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGTTAGTGCCAGAACACCCGACATATCGGGGCCAGCGTCAGGCCGCCA                                  >  SRR4302540.2341287/1‑94 (MQ=60)
                                                              TGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAG                                 >  SRR4302540.8713910/1‑94 (MQ=60)
                                                               GAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGT                                <  SRR4302540.5270607/94‑1 (MQ=60)
                                                                 GAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTA                              <  SRR4302540.3128586/94‑1 (MQ=60)
                                                                 AAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTA                              <  SRR4302540.6242466/94‑1 (MQ=60)
                                                                   AACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAA                            <  SRR4302540.9779686/94‑1 (MQ=60)
                                                                     CCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGT                          >  SRR4302540.8073540/1‑94 (MQ=60)
                                                                      CAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTC                         >  SRR4302540.4689074/1‑94 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAATTCA                        >  SRR4302540.2117376/1‑94 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCA                        <  SRR4302540.10077444/94‑1 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGAGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCA                        >  SRR4302540.3622575/1‑94 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       >  SRR4302540.1775864/1‑94 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       <  SRR4302540.2926224/94‑1 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       <  SRR4302540.9652396/94‑1 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       <  SRR4302540.2926223/94‑1 (MQ=60)
                                                                          GACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCA                     >  SRR4302540.6715622/1‑94 (MQ=60)
                                                                          GACGGACCGAGCGGCAGCCGGGTGGTATTGGCGAGATAGTGCCAGAACACCAGCCATATCGGGGCCAGCGTCAGGCCGCCCGATAAAGTCAGCA                     >  SRR4302540.9162296/1‑94 (MQ=60)
                                                                           ACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAA                    <  SRR4302540.3733618/94‑1 (MQ=60)
                                                                           ACGGACCGAGCGACAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAA                    <  SRR4302540.8079145/94‑1 (MQ=60)
                                                                            CGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAAC                   >  SRR4302540.3586773/1‑94 (MQ=60)
                                                                            CGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAAC                   <  SRR4302540.8402849/94‑1 (MQ=60)
                                                                             GGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCTGACATATCGGGTCCAGCGTCATGCCGCCATCTAAAGTCAGCAACA                  >  SRR4302540.9066186/1‑94 (MQ=60)
                                                                             GGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACA                  >  SRR4302540.1898305/1‑94 (MQ=60)
                                                                             GGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACA                  >  SRR4302540.9962858/1‑94 (MQ=60)
                                                                             GGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACA                  >  SRR4302540.10199/1‑94 (MQ=60)
                                                                              GACCGAGCGGCAGTCGGGTTTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCCGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 <  SRR4302540.2471690/94‑1 (MQ=60)
                                                                              GACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 >  SRR4302540.6789581/1‑94 (MQ=60)
                                                                              GACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 <  SRR4302540.2471691/94‑1 (MQ=60)
                                                                              GACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 <  SRR4302540.5931979/94‑1 (MQ=60)
                                                                              GACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 >  SRR4302540.1485672/1‑94 (MQ=60)
                                                                               ACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGC                <  SRR4302540.2231678/94‑1 (MQ=60)
                                                                               ACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGC                >  SRR4302540.6809097/1‑94 (MQ=60)
                                                                                CCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCG               <  SRR4302540.845848/94‑1 (MQ=60)
                                                                                 CGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGC              <  SRR4302540.6672906/94‑1 (MQ=60)
                                                                                 CGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGA              >  SRR4302540.2599273/1‑94 (MQ=60)
                                                                                  GAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCA             <  SRR4302540.3682134/94‑1 (MQ=60)
                                                                                   GGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCGGGCATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAT            <  SRR4302540.9814564/94‑1 (MQ=60)
                                                                                   AGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAT            >  SRR4302540.4570886/1‑94 (MQ=60)
                                                                                     CGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTC          >  SRR4302540.4222943/1‑94 (MQ=60)
                                                                                     CGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTC          <  SRR4302540.3549231/94‑1 (MQ=60)
                                                                                     CGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTC          >  SRR4302540.9707642/1‑94 (MQ=60)
                                                                                      GGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCC         <  SRR4302540.6626628/94‑1 (MQ=60)
                                                                                      GGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCC         <  SRR4302540.5549539/94‑1 (MQ=60)
                                                                                       GCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCA        <  SRR4302540.360287/94‑1 (MQ=60)
                                                                                       GCAGCCGGGTGTTATTGGCGAGATAGCGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCA        <  SRR4302540.2310345/94‑1 (MQ=60)
                                                                                          GCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGA     >  SRR4302540.7528021/1‑94 (MQ=60)
                                                                                           CCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGAC    <  SRR4302540.7376364/94‑1 (MQ=60)
                                                                                           CCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGAC    >  SRR4302540.2045524/1‑94 (MQ=60)
                                                                                            CGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACT   <  SRR4302540.861601/94‑1 (MQ=60)
                                                                                            CGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACT   <  SRR4302540.3198311/94‑1 (MQ=60)
                                                                                             GGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  SRR4302540.8825909/1‑94 (MQ=60)
                                                                                             GGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  SRR4302540.4948172/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  NC_000913/3847172‑3847358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑