Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,857,508 T→C E79G (GAA→GGA)  yidK ← putative cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,857,5080TC99.6% 1508.3 / ‑3.5 467E79G (GAA→GGA) yidKputative cotransporter
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (234/231);  total (235/232)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 481 total aligned reads displayed.

AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597
                                                                                          |                                                                                         
aGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTccc                                                                                         >  2:748472/1‑93 (MQ=255)
  aaaaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCa                                                                                       <  2:1441302/93‑1 (MQ=255)
  aaaaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCa                                                                                       >  1:7748259/1‑93 (MQ=255)
   aactCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAg                                                                                      <  1:1731796/90‑1 (MQ=255)
    aaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGc                                                                                     >  1:7901691/1‑93 (MQ=255)
    aaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGc                                                                                     <  1:3899973/93‑1 (MQ=255)
      tCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGccc                                                                                   >  2:1201715/1‑93 (MQ=255)
        ggggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAt                                                                                 >  1:6161808/1‑93 (MQ=255)
         gggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATc                                                                                >  2:695957/1‑93 (MQ=255)
          ggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATcc                                                                               >  2:7247050/1‑92 (MQ=255)
          ggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCa                                                                               <  1:9727537/93‑1 (MQ=255)
           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAc                                                                              <  2:4476645/93‑1 (MQ=255)
            aTGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAcc                                                                             <  1:1043281/93‑1 (MQ=255)
            aTGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAcc                                                                             <  2:9290349/93‑1 (MQ=255)
            aTGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGCCACCGCTGAAGTCACTCCCCAGCCCATCAcc                                                                             <  1:7280346/93‑1 (MQ=255)
             tggtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCg                                                                            >  1:6597811/1‑93 (MQ=255)
             tggtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCg                                                                            >  2:6110444/1‑93 (MQ=255)
              ggtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGa                                                                           >  2:8404877/1‑93 (MQ=255)
               gtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAc                                                                          <  2:6268397/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:3407078/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  2:5047159/93‑1 (MQ=255)
                tgacAATTCCCCGCTTCAGATAGCCCCGTAAAAAGATTAGCGCGAGGAAGATCAGCTTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:2460520/90‑1 (MQ=255)
                   gaaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGc                                                                      <  2:7545456/92‑1 (MQ=255)
                    aaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGcc                                                                     <  1:8327477/93‑1 (MQ=255)
                     aTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCg                                                                    >  1:4278371/1‑93 (MQ=255)
                      tGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGc                                                                   >  1:9870915/1‑93 (MQ=255)
                         cgcgCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCttt                                                                >  1:9268976/1‑93 (MQ=255)
                         cgcgCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCttt                                                                >  2:9403253/1‑93 (MQ=255)
                          gcgcTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCtttt                                                               >  2:6537241/1‑93 (MQ=255)
                             cTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTa                                                            >  1:4902934/1‑93 (MQ=255)
                             cTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTa                                                            <  2:2582041/93‑1 (MQ=255)
                              ttCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAg                                                           <  2:4534258/93‑1 (MQ=255)
                               tCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAgg                                                          >  2:3006512/1‑93 (MQ=255)
                                cAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCCCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGc                                                         <  2:9825421/93‑1 (MQ=255)
                                 agagagCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGcc                                                        <  2:4653319/93‑1 (MQ=255)
                                  gATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCt                                                       >  1:8875910/1‑93 (MQ=255)
                                  gATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCt                                                       >  2:7148080/1‑93 (MQ=255)
                                     aGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGcc                                                    <  2:6395309/93‑1 (MQ=255)
                                        gcgGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCgg                                                 >  1:6385618/1‑93 (MQ=255)
                                          ggTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                               <  1:3667990/93‑1 (MQ=255)
                                          ggTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                               <  1:4671025/93‑1 (MQ=255)
                                          ggTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGACCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                               >  1:5336761/1‑93 (MQ=255)
                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              >  1:4575029/1‑93 (MQ=255)
                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              >  2:7872893/1‑93 (MQ=255)
                                            tAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAg                                             <  1:8773651/93‑1 (MQ=255)
                                            tAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAg                                             <  2:9524355/93‑1 (MQ=255)
                                            tAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAg                                             <  1:1874235/93‑1 (MQ=255)
                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAgg                                            >  1:4869464/1‑93 (MQ=255)
                                              aaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGc                                           >  1:8583139/1‑93 (MQ=255)
                                              aaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGc                                           <  2:3634398/93‑1 (MQ=255)
                                              aaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGc                                           <  2:2244579/93‑1 (MQ=255)
                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCGTCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          >  2:5875747/1‑93 (MQ=255)
                                                aaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCg                                         >  2:1620700/1‑93 (MQ=255)
                                                aaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCg                                         >  1:6880628/1‑93 (MQ=255)
                                                aaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCg                                         >  1:149235/1‑93 (MQ=255)
                                                 agcttAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGa                                        >  2:4297211/4‑93 (MQ=255)
                                                 aGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGa                                        >  2:3540585/1‑93 (MQ=255)
                                                 aGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGa                                        >  1:10043121/1‑93 (MQ=255)
                                                  gATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAc                                       >  2:6049957/1‑93 (MQ=255)
                                                     tAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAg                                    >  2:6904259/1‑93 (MQ=255)
                                                       gcgcGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgtt                                  <  1:2891304/93‑1 (MQ=255)
                                                          cgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttgtt                               <  2:8860894/93‑1 (MQ=255)
                                                           gAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTc                              >  2:3780282/1‑93 (MQ=255)
                                                           gAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTc                              <  2:4071818/93‑1 (MQ=255)
                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             >  1:9558919/1‑93 (MQ=255)
                                                             ggAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCg                            >  2:859196/1‑93 (MQ=255)
                                                             ggAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCg                            >  1:898051/1‑93 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           <  2:7370673/93‑1 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           >  2:5598277/1‑93 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           <  1:6811438/93‑1 (MQ=255)
                                                               aaGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTg                          >  1:5417794/1‑93 (MQ=255)
                                                                 gATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGa                        >  1:982835/1‑93 (MQ=255)
                                                                   tCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaaa                      <  1:8100427/93‑1 (MQ=255)
                                                                     aGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgg                    >  2:9108920/1‑93 (MQ=255)
                                                                      gCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggt                   <  1:1208263/93‑1 (MQ=255)
                                                                      gCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggt                   <  2:5403049/93‑1 (MQ=255)
                                                                       cctCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgggt                  <  1:2134792/91‑3 (MQ=255)
                                                                       cGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggtt                  <  2:3415476/93‑1 (MQ=255)
                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  1:2770328/1‑93 (MQ=255)
                                                                         tCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttgg                <  2:1027708/93‑1 (MQ=255)
                                                                         tCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttgg                >  2:9648214/1‑93 (MQ=255)
                                                                         tCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttgg                >  2:8108994/1‑93 (MQ=255)
                                                                          cACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggt               <  2:3284809/93‑1 (MQ=255)
                                                                          cACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggt               <  1:7492923/93‑1 (MQ=255)
                                                                           cccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggtt              <  1:5167710/92‑1 (MQ=255)
                                                                           cccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggtt              <  2:3170765/92‑1 (MQ=255)
                                                                           aCCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggtt              <  1:1330892/93‑1 (MQ=255)
                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTa             <  1:3023823/93‑1 (MQ=255)
                                                                              gCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGc           >  2:7699203/1‑93 (MQ=255)
                                                                               cTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCa          <  2:673509/93‑1 (MQ=255)
                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         >  1:2233046/1‑93 (MQ=255)
                                                                                 gAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGCAAGGCCGACCAGCTGTTCCGTGGAAAGGTTGGTTAGCAtt        >  1:5554179/1‑93 (MQ=255)
                                                                                 gAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAtt        >  1:7103258/1‑93 (MQ=255)
                                                                                  aaGTCACTCCCCAGCCCATCACCGACATGGCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTa       >  2:8944545/1‑93 (MQ=255)
                                                                                  aaGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTa       >  2:2055053/1‑93 (MQ=255)
                                                                                  aaGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTa       <  2:9214049/93‑1 (MQ=255)
                                                                                   aGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTaa      <  1:3268200/93‑1 (MQ=255)
                                                                                   aGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTaa      >  1:5666501/1‑93 (MQ=255)
                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     >  2:8220467/1‑93 (MQ=255)
                                                                                       aCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGaa  <  1:4025449/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 246 total aligned reads displayed.
TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCGG  >  NC_000913/3857415‑3857601
                                                                                             |                                                                                             
  CAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCC                                                                                             >  SRR4302540.8159778/1‑94 (MQ=60)
    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCA                                                                                           <  SRR4302540.4110165/94‑1 (MQ=60)
    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCA                                                                                           <  SRR4302540.1201057/94‑1 (MQ=60)
    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCA                                                                                           >  SRR4302540.7838724/1‑94 (MQ=60)
     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAG                                                                                          >  SRR4302540.2590475/1‑94 (MQ=60)
      AACTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGC                                                                                         <  SRR4302540.1750486/94‑1 (MQ=60)
      AAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGC                                                                                         >  SRR4302540.7993847/1‑94 (MQ=60)
       AATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCC                                                                                        <  SRR4302540.3942291/94‑1 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                       >  SRR4302540.7355859/1‑94 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                       >  SRR4302540.9965399/1‑94 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                       <  SRR4302540.1380403/94‑1 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                       <  SRR4302540.5957820/94‑1 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                       <  SRR4302540.7786016/94‑1 (MQ=60)
         TCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCA                                                                                      <  SRR4302540.5432972/94‑1 (MQ=60)
          CGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAT                                                                                     >  SRR4302540.6230470/1‑94 (MQ=60)
           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATC                                                                                    <  SRR4302540.8549738/94‑1 (MQ=60)
           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATC                                                                                    >  SRR4302540.4054099/1‑94 (MQ=60)
            GGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCA                                                                                   <  SRR4302540.6934683/94‑1 (MQ=60)
            GGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCA                                                                                   >  SRR4302540.6369293/1‑94 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACGGCTGAAGTCACTCCCCAGCCCATCAC                                                                                  >  SRR4302540.4550374/1‑94 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                  <  SRR4302540.9841780/94‑1 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                  >  SRR4302540.5581019/1‑94 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                  <  SRR4302540.6730223/94‑1 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                  >  SRR4302540.1928100/1‑94 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                 <  SRR4302540.2490044/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                 <  SRR4302540.1391526/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                 <  SRR4302540.4750836/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                 <  SRR4302540.395987/94‑1 (MQ=60)
               ATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCG                                                                                >  SRR4302540.6670869/1‑94 (MQ=60)
                TGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAGAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGA                                                                               <  SRR4302540.4743000/94‑1 (MQ=60)
                TGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGA                                                                               <  SRR4302540.6987238/94‑1 (MQ=60)
                TGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGA                                                                               >  SRR4302540.3579638/1‑94 (MQ=60)
                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGTGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAC                                                                              <  SRR4302540.1322019/94‑1 (MQ=60)
                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAC                                                                              >  SRR4302540.6657158/1‑94 (MQ=60)
                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAC                                                                              >  SRR4302540.4697925/1‑94 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                            >  SRR4302540.3054990/1‑94 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                            <  SRR4302540.3444198/94‑1 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                            <  SRR4302540.120109/94‑1 (MQ=60)
                   TGACAATTCCCCGCTTCAGATAGCCCCGTAAAAAGATTAGCGCGAGGAAGATCAGCTTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                            <  SRR4302540.2487006/94‑1 (MQ=60)
                   GGGCAATGCGGCGCTGCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                            <  SRR4302540.7868961/94‑1 (MQ=60)
                    GGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATG                                                                           <  SRR4302540.8375077/94‑1 (MQ=60)
                     GCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGC                                                                          >  SRR4302540.368080/1‑94 (MQ=60)
                     GCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGC                                                                          >  SRR4302540.7492584/1‑94 (MQ=60)
                     GCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGC                                                                          >  SRR4302540.7921120/1‑94 (MQ=60)
                      CAATGCCGCGGTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCC                                                                         >  SRR4302540.10007192/1‑94 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        >  SRR4302540.1028907/1‑94 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        >  SRR4302540.4324905/1‑94 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        <  SRR4302540.1023333/94‑1 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        <  SRR4302540.5368543/94‑1 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        <  SRR4302540.8424130/94‑1 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                        >  SRR4302540.730766/1‑94 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGCCACTCCCCAGCCCATCACCGACATGCCG                                                                        >  SRR4302540.1028908/1‑94 (MQ=60)
                          GCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTT                                                                     <  SRR4302540.9029874/94‑1 (MQ=60)
                           CCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTT                                                                    >  SRR4302540.9379823/1‑94 (MQ=60)
                            CGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTT                                                                   <  SRR4302540.2184785/94‑1 (MQ=60)
                             GCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTG                                                                  <  SRR4302540.838294/94‑1 (MQ=60)
                             GCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTG                                                                  <  SRR4302540.9211425/94‑1 (MQ=60)
                             GCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTG                                                                  >  SRR4302540.4750993/1‑94 (MQ=60)
                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTA                                                                >  SRR4302540.5717186/1‑94 (MQ=60)
                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTA                                                                >  SRR4302540.4955687/1‑94 (MQ=60)
                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTA                                                                >  SRR4302540.624683/1‑94 (MQ=60)
                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTA                                                                >  SRR4302540.5731668/1‑94 (MQ=60)
                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAG                                                               <  SRR4302540.5584716/94‑1 (MQ=60)
                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAG                                                               >  SRR4302540.9594090/1‑94 (MQ=60)
                                 TTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGG                                                              <  SRR4302540.7068098/94‑1 (MQ=60)
                                 TTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGG                                                              >  SRR4302540.6281266/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                           >  SRR4302540.4397470/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                           >  SRR4302540.3870843/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                           >  SRR4302540.8978632/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                           >  SRR4302540.1207187/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                           <  SRR4302540.879851/94‑1 (MQ=60)
                                     GATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTG                                                          >  SRR4302540.2395557/1‑94 (MQ=60)
                                         GCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCG                                                      <  SRR4302540.7416231/94‑1 (MQ=60)
                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGG                                                     >  SRR4302540.6456704/1‑94 (MQ=60)
                                           GCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGA                                                    >  SRR4302540.5002633/1‑94 (MQ=60)
                                            CGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAA                                                   >  SRR4302540.3768668/1‑94 (MQ=60)
                                            CGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAA                                                   >  SRR4302540.3081781/1‑94 (MQ=60)
                                            CGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAA                                                   >  SRR4302540.6190448/1‑94 (MQ=60)
                                            CGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGACCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAA                                                   >  SRR4302540.5395262/1‑94 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                  >  SRR4302540.4624383/1‑94 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                  <  SRR4302540.4721017/94‑1 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                  <  SRR4302540.3708115/94‑1 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                  >  SRR4302540.9771248/1‑94 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                 <  SRR4302540.4341875/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                 >  SRR4302540.5423860/1‑94 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                 >  SRR4302540.3324439/1‑94 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                 <  SRR4302540.2736754/94‑1 (MQ=60)
                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                                <  SRR4302540.8875296/94‑1 (MQ=60)
                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                                <  SRR4302540.1894765/94‑1 (MQ=60)
                                               GAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCCCCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                                <  SRR4302540.5139772/94‑1 (MQ=60)
                                                AAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGC                                               >  SRR4302540.4635356/1‑94 (MQ=60)
                                                AAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGC                                               >  SRR4302540.9423436/1‑94 (MQ=60)
                                                AAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGC                                               >  SRR4302540.8682918/1‑94 (MQ=60)
                                                AAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTAACTCCTCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGC                                               >  SRR4302540.9289738/1‑94 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                              <  SRR4302540.9674184/94‑1 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                              <  SRR4302540.5472060/94‑1 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                              >  SRR4302540.7631014/1‑94 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                             >  SRR4302540.150775/1‑94 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                             >  SRR4302540.6956899/1‑94 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                             >  SRR4302540.4860089/1‑94 (MQ=60)
                                                   AAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGA                                            >  SRR4302540.2280168/1‑94 (MQ=60)
                                                   AAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGA                                            >  SRR4302540.10159883/1‑94 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                           <  SRR4302540.871264/94‑1 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                           <  SRR4302540.6970034/94‑1 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                           >  SRR4302540.2067712/1‑94 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAAGCCGCTGTTGTAGGCCTGCCCGGAAAGGCCGAC                                           >  SRR4302540.4932165/1‑94 (MQ=60)
                                                     GATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCaaac                                          >  SRR4302540.9180869/1‑90 (MQ=60)
                                                     GATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACC                                          >  SRR4302540.2956014/1‑94 (MQ=60)
                                                     GATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACC                                          >  SRR4302540.2956015/1‑94 (MQ=60)
                                                      ATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCA                                         >  SRR4302540.1807006/1‑94 (MQ=60)
                                                      ATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCA                                         <  SRR4302540.2885179/94‑1 (MQ=60)
                                                       aaAGCGCGGGGAAGATTAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAG                                        <  SRR4302540.2448277/92‑1 (MQ=60)
                                                       TTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAG                                        <  SRR4302540.123096/94‑1 (MQ=60)
                                                        TAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGT                                       >  SRR4302540.8353032/1‑94 (MQ=60)
                                                        TAGCGCGAGGAAGATCAGCGTCACCGCTGAAGACACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGT                                       >  SRR4302540.2087953/1‑94 (MQ=60)
                                                         AGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTT                                      <  SRR4302540.6814047/94‑1 (MQ=60)
                                                          GCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTG                                     <  SRR4302540.2922814/94‑1 (MQ=60)
                                                           CGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGT                                    <  SRR4302540.5255635/94‑1 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                   >  SRR4302540.3960259/1‑94 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                   <  SRR4302540.7105893/94‑1 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                   <  SRR4302540.1453583/94‑1 (MQ=60)
                                                             CGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTC                                  >  SRR4302540.9849037/1‑94 (MQ=60)
                                                             CGAGGAAAATCAGCGTCACCGCTGAAGTCCCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTC                                  <  SRR4302540.7822635/94‑1 (MQ=60)
                                                              GAGGACGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                 <  SRR4302540.9185651/94‑1 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                 <  SRR4302540.5672664/94‑1 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                 <  SRR4302540.5587920/94‑1 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                 >  SRR4302540.7266637/1‑94 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                 >  SRR4302540.9671938/1‑94 (MQ=60)
                                                               AGGAAGTTCAGCGTCACCGCCGAAGTCACTCCCCAGCCCATCACCGACTTGCCGCTTTTGGAGGCCTGCTCGGAAAGGCCGACCAGTTGTTCCG                                >  SRR4302540.9078423/1‑94 (MQ=60)
                                                                 GAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTG                              >  SRR4302540.6706946/1‑94 (MQ=60)
                                                                 GAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTG                              <  SRR4302540.6886803/94‑1 (MQ=60)
                                                                 GAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTG                              >  SRR4302540.5477368/1‑94 (MQ=60)
                                                                  AAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGG                             >  SRR4302540.1769123/1‑94 (MQ=60)
                                                                  AAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGG                             <  SRR4302540.8876073/94‑1 (MQ=60)
                                                                   AGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGA                            >  SRR4302540.992896/1‑94 (MQ=60)
                                                                     ATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAA                          >  SRR4302540.4944900/1‑94 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                         <  SRR4302540.8194444/94‑1 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                         <  SRR4302540.3755286/94‑1 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                         <  SRR4302540.2211121/94‑1 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                         <  SRR4302540.6589064/94‑1 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                         <  SRR4302540.1577906/94‑1 (MQ=60)
                                                                       CAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGG                        <  SRR4302540.7358118/94‑1 (MQ=60)
                                                                        AGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                       >  SRR4302540.2851029/1‑94 (MQ=60)
                                                                        AGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                       <  SRR4302540.98817/94‑1 (MQ=60)
                                                                         GCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                      <  SRR4302540.8794993/94‑1 (MQ=60)
                                                                         GCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                      <  SRR4302540.7401976/94‑1 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACTTGCCGCTTTTGAATGCCTGCCCGGAAAGGCCGACCAGGTGTTCCGTGAAAAGTTTG                     >  SRR4302540.2800264/1‑94 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                     >  SRR4302540.8472894/1‑94 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                     <  SRR4302540.4179792/94‑1 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                     >  SRR4302540.2800265/1‑94 (MQ=60)
                                                                          CCTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGGTG                     <  SRR4302540.2157705/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                    <  SRR4302540.7457128/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                    >  SRR4302540.9031424/1‑94 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                    >  SRR4302540.6906285/1‑94 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGGTGG                    >  SRR4302540.9954257/1‑94 (MQ=60)
                                                                             CACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTT                  <  SRR4302540.3340799/94‑1 (MQ=60)
                                                                             CACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTT                  <  SRR4302540.1272100/94‑1 (MQ=60)
                                                                             CACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTT                  >  SRR4302540.8955389/1‑94 (MQ=60)
                                                                              CCCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                 <  SRR4302540.8170489/94‑1 (MQ=60)
                                                                              CCCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                 <  SRR4302540.5223919/94‑1 (MQ=60)
                                                                              ACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                 <  SRR4302540.1344802/94‑1 (MQ=60)
                                                                              ACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                 <  SRR4302540.1142662/94‑1 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG                >  SRR4302540.2338455/1‑94 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG                <  SRR4302540.3057043/94‑1 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG                <  SRR4302540.9621348/94‑1 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG                <  SRR4302540.5130318/94‑1 (MQ=60)
                                                                                CGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC               >  SRR4302540.9863860/1‑94 (MQ=60)
                                                                                CGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC               <  SRR4302540.1277585/94‑1 (MQ=60)
                                                                                CGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC               >  SRR4302540.8902466/1‑94 (MQ=60)
                                                                                 GCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCA              >  SRR4302540.8427539/1‑94 (MQ=60)
                                                                                 GCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCA              >  SRR4302540.5300513/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT             >  SRR4302540.2257080/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGATGGTTAGCAT             >  SRR4302540.9972971/1‑94 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGCAAGGCCGACCAGCTGTTCCGTGGAAAGGTTGGTTAGCATT            >  SRR4302540.5615354/1‑94 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT            >  SRR4302540.1663365/1‑94 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT            <  SRR4302540.1796914/94‑1 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT            >  SRR4302540.4924177/1‑94 (MQ=60)
                                                                                    GAAGTCACTCCCCGGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA           <  SRR4302540.9769653/94‑1 (MQ=60)
                                                                                    GAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA           <  SRR4302540.1553383/94‑1 (MQ=60)
                                                                                    GAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA           <  SRR4302540.7618848/94‑1 (MQ=60)
                                                                                     AAGTCACTCCCCAGCGCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA          <  SRR4302540.4183555/94‑1 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA          >  SRR4302540.5728905/1‑94 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA          >  SRR4302540.3391757/1‑94 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAACGCCGACCAGTTGTTCCGTGGTAAGGCTGGTTAGCATTAA          >  SRR4302540.5234299/1‑94 (MQ=60)
                                                                                      AGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC         >  SRR4302540.8031537/1‑94 (MQ=60)
                                                                                      AGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC         <  SRR4302540.3303870/94‑1 (MQ=60)
                                                                                      AGTAACTCCCCAGCCCATCACGGCGATGCCGCTTTTGTAGGCCGGCACGGAAAGGGTGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC         <  SRR4302540.3303869/94‑1 (MQ=57)
                                                                                       GTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG        <  SRR4302540.4656762/94‑1 (MQ=60)
                                                                                       GTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG        >  SRR4302540.3140103/1‑94 (MQ=60)
                                                                                       GTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG        <  SRR4302540.2160056/94‑1 (MQ=60)
                                                                                       GTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG        >  SRR4302540.6566770/1‑94 (MQ=60)
                                                                                        TCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGA       >  SRR4302540.3555340/1‑94 (MQ=60)
                                                                                          ACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG     <  SRR4302540.4068852/94‑1 (MQ=60)
                                                                                          ACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG     >  SRR4302540.2956454/1‑94 (MQ=60)
                                                                                          ACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG     >  SRR4302540.8146818/1‑94 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG   <  SRR4302540.3422315/94‑1 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG   >  SRR4302540.612593/1‑94 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCAGTGGAAAGGTTGGTTAGCATTAACGAAGCG   >  SRR4302540.2106611/1‑94 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGGTGTTCAGTGGAAAGGTTGGTTAGCATTAACGAAGCG   >  SRR4302540.2106612/1‑94 (MQ=60)
                                                                                             CCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCGG  <  SRR4302540.227767/94‑1 (MQ=60)
                                                                                             |                                                                                             
TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCGG  >  NC_000913/3857415‑3857601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑