Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,975 G→A A452V (GCA→GTA)  bglB ← phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,9750GA99.7% 2337.6 / NA 642A452V (GCA→GTA) bglBphospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (294/345);  minor base C (2/0);  total (297/345)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3899885‑3900064
                                                                                             |                                                                                         
 cgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACa                                                                                         <  1:7417526/93‑1 (MQ=255)
 cgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACa                                                                                         <  2:9289844/93‑1 (MQ=255)
    atcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATAc                                                                                      <  2:1622061/93‑1 (MQ=255)
     tcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATAcc                                                                                     >  2:4706980/1‑93 (MQ=255)
        aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATc                                                                                  <  2:3009212/93‑1 (MQ=255)
         gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATcc                                                                                 <  1:7587738/93‑1 (MQ=255)
         gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATcc                                                                                 <  1:6927916/93‑1 (MQ=255)
         gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATcc                                                                                 >  2:6381173/1‑93 (MQ=255)
           cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGaa                                                                              <  2:3372111/93‑1 (MQ=255)
            tGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAg                                                                             >  1:2943420/1‑93 (MQ=255)
            tGTTCGGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGaa                                                                              <  2:7925688/93‑1 (MQ=255)
             gTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAg                                                                             >  2:2718085/1‑93 (MQ=255)
               tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCt                                                                           >  1:6540032/1‑93 (MQ=255)
               tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCt                                                                           >  2:9161614/1‑93 (MQ=255)
                 tGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCttt                                                                         >  1:676109/1‑93 (MQ=255)
                 tGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCttt                                                                         >  1:2060270/1‑93 (MQ=255)
                  gAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCtttt                                                                        >  1:880594/1‑93 (MQ=255)
                    aTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCt                                                                      <  2:1072834/93‑1 (MQ=255)
                    aTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTTCATGCCATCCGAAGCTTTTCtt                                                                     >  2:113053/1‑93 (MQ=255)
                     tGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCtt                                                                     <  2:8377195/93‑1 (MQ=255)
                     tGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTCCATACCATACGAAGCTTTTCTTg                                                                    >  2:7178962/1‑93 (MQ=255)
                     tGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTg                                                                    >  2:4205961/1‑93 (MQ=255)
                       cgacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGc                                                                   <  1:3498409/93‑1 (MQ=255)
                       cgacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGc                                                                   <  1:3131154/93‑1 (MQ=255)
                       cgacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCg                                                                  <  1:509676/93‑1 (MQ=255)
                         acgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGACCCCCGCGTCCTGATCACC‑TCTTCATACCATGCCAAGCTTTTCTTGcct                                                                 >  2:4048441/1‑91 (MQ=255)
                         acgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCgtg                                                                <  1:4381498/93‑1 (MQ=255)
                           gaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGtt                                                              >  1:8670558/1‑93 (MQ=255)
                            aTAATTAAGGTGCTTTAATGGTTATTTTTTTTTAATGCCAC‑CCCCCCCGTTTTGA‑CACCTTCTACATACAATCCGCGGCTTTTCTGGCGGGtt                                                              >  2:2457044/1‑93 (MQ=255)
                             tAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTc                                                             >  1:9876851/1‑93 (MQ=255)
                              aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                                            >  1:9246662/1‑93 (MQ=255)
                              aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                                            <  2:9598467/93‑1 (MQ=255)
                               aTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                                          <  1:8278973/93‑1 (MQ=255)
                                ttAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                                          >  2:9668060/1‑93 (MQ=255)
                                  aaGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       >  1:2443151/1‑93 (MQ=255)
                                   aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  2:8217022/93‑1 (MQ=255)
                                   aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  1:4132252/93‑1 (MQ=255)
                                   aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  2:36290/93‑1 (MQ=255)
                                   aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  2:6194140/93‑1 (MQ=255)
                                      tGCTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGc                                                    <  2:5650767/93‑1 (MQ=255)
                                      tGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                                                   <  1:3668866/93‑1 (MQ=255)
                                      tGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                                                   >  2:2341210/1‑93 (MQ=255)
                                       gCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGctt                                                  >  1:1250852/1‑93 (MQ=255)
                                       gCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGctt                                                  >  2:4816116/1‑93 (MQ=255)
                                        cTTTAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGctt                                                  <  2:4289220/93‑1 (MQ=255)
                                         tttAATGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                                                 <  2:1819717/93‑1 (MQ=255)
                                          ttAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct                                               <  2:2208114/93‑1 (MQ=255)
                                          ttAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct                                               <  1:778333/93‑1 (MQ=255)
                                           tAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTTTTGCGTGTTCTTGTGAGGcttcctt                                              >  1:1457609/1‑93 (MQ=255)
                                           tAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              >  2:2007881/1‑93 (MQ=255)
                                            aaTGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcttt                                              >  2:8963767/1‑93 (MQ=255)
                                            aaTGGTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              <  1:8510937/93‑1 (MQ=255)
                                            aaTGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttccttc                                             <  1:9451852/93‑1 (MQ=255)
                                                gTTA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGcc                                          >  1:3941284/1‑93 (MQ=255)
                                                 ttA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                                         <  1:2678208/93‑1 (MQ=255)
                                                  tA‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAt                                        <  2:4487264/93‑1 (MQ=255)
                                                   a‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt                                       <  1:1101654/93‑1 (MQ=255)
                                                   a‑TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt                                       >  1:3286060/1‑93 (MQ=255)
                                                   a‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa                                      >  1:10046127/1‑93 (MQ=255)
                                                   a‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa                                      <  1:9045461/93‑1 (MQ=255)
                                                     ttttttttAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa                                      <  1:7065225/93‑1 (MQ=255)
                                                     ttttttttAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTat                                     <  1:5797933/93‑1 (MQ=255)
                                                      tttttttAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTat                                     >  1:6321877/1‑93 (MQ=255)
                                                        tttttAATGACAGCCCCCCGCGTCTTGATCCCC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca                                   <  1:4928457/93‑1 (MQ=255)
                                                        tttttAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca                                   >  2:6084358/1‑93 (MQ=255)
                                                         ttttAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcat                                  >  1:2629705/1‑93 (MQ=255)
                                                         ttttAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcatc                                 <  1:794302/93‑1 (MQ=255)
                                                          tttAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca                                <  1:5971876/93‑1 (MQ=255)
                                                           ttAATGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGATTCCTTCGCCATTAtcatca                                <  1:5218160/93‑1 (MQ=255)
                                                           ttAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAc                               >  1:973263/1‑93 (MQ=255)
                                                              aTGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAt                            <  2:314532/93‑1 (MQ=255)
                                                              aTGACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAt                            >  2:1241622/1‑93 (MQ=255)
                                                               tGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAt                            <  1:9136028/93‑1 (MQ=255)
                                                               tGACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAt                            >  2:5020554/1‑93 (MQ=255)
                                                                gACAGCCCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATc                           <  2:7559755/93‑1 (MQ=255)
                                                                gACAG‑CCCCCGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATcc                          >  2:4881875/1‑93 (MQ=255)
                                                                  cagccccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCca                         >  2:532538/5‑93 (MQ=255)
                                                                   atccccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        <  1:614299/90‑1 (MQ=255)
                                                                   agccccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        <  1:1708854/90‑1 (MQ=255)
                                                                    gccccccGCGGATTCATCACC‑CTTACATACCCTCCCAAACTTTTCTTGCGTGTTCTTGTGAGGCTTACCTTGTCCTTTTTATCACGTACcaca                       >  2:7294139/3‑93 (MQ=255)
                                                                      cccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACata                     >  1:1606384/1‑93 (MQ=255)
                                                                      cccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACata                     <  1:2229245/93‑1 (MQ=255)
                                                                       ccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataa                    >  2:3859898/1‑93 (MQ=255)
                                                                       ccccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataa                    <  1:8617018/93‑1 (MQ=255)
                                                                        cccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaa                   >  1:4707515/1‑93 (MQ=255)
                                                                         ccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaat                  <  2:3281998/93‑1 (MQ=255)
                                                                         ccGCGTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaat                  <  1:9587541/93‑1 (MQ=255)
                                                                           gcgTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaataa                >  2:8809307/1‑93 (MQ=255)
                                                                             gTCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAg              <  1:3398044/93‑1 (MQ=255)
                                                                              tCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             <  1:1790278/93‑1 (MQ=255)
                                                                              tCTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             >  2:589224/1‑93 (MQ=255)
                                                                              gcTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             <  1:5603635/92‑1 (MQ=255)
                                                                               cTTGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGcc            <  2:8085326/93‑1 (MQ=255)
                                                                                 tgatgaCC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATTAATAAAGCCGt          <  2:8608459/93‑1 (MQ=255)
                                                                                 tGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGt          <  1:5451562/93‑1 (MQ=255)
                                                                                 tGATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGt          <  1:8073761/93‑1 (MQ=255)
                                                                                  gATCACC‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         <  2:6787566/93‑1 (MQ=255)
                                                                                       cc‑TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCt    >  1:3847658/1‑93 (MQ=255)
                                                                                          tctACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTa  >  1:7236830/1‑93 (MQ=255)
                                                                                          tctACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTa  >  2:6913363/1‑93 (MQ=255)
                                                                                             |                                                                                         
TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACC‑TCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3899885‑3900064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 387 total aligned reads displayed.
GCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  NC_000913/3899882‑3900068
                                                                                                 |                                                                                             
GCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTA                                                                                               >  SRR4302540.8106602/1‑94 (MQ=60)
 CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTA                                                                                               <  SRR4302540.1002454/94‑1 (MQ=60)
 CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAAAGC‑CC‑C‑CCGCGTCTTGATCACCTCTA                                                                                               >  SRR4302540.8760192/1‑94 (MQ=60)
  ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTAC                                                                                              >  SRR4302540.4185738/1‑94 (MQ=60)
  ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTAC                                                                                              <  SRR4302540.5970657/94‑1 (MQ=60)
  ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTAC                                                                                              >  SRR4302540.8371156/1‑94 (MQ=60)
  ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACA                                                                                             >  SRR4302540.7545916/1‑94 (MQ=60)
   TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACA                                                                                             <  SRR4302540.7046142/94‑1 (MQ=60)
   TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACAT                                                                                            >  SRR4302540.7865055/1‑94 (MQ=60)
   TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACAT                                                                                            <  SRR4302540.2734848/94‑1 (MQ=60)
    CGCTTCCAGACTGTTCTTAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACAT                                                                                            <  SRR4302540.3211609/94‑1 (MQ=60)
    CGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACAT                                                                                            <  SRR4302540.7500145/94‑1 (MQ=60)
     GCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATA                                                                                           >  SRR4302540.7721854/1‑94 (MQ=60)
     GCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATA                                                                                           >  SRR4302540.9860889/1‑94 (MQ=60)
     GCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATAC                                                                                          <  SRR4302540.8698349/94‑1 (MQ=60)
      CATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATAC                                                                                          >  SRR4302540.1394174/1‑94 (MQ=60)
      CATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACC                                                                                         >  SRR4302540.6649198/1‑94 (MQ=60)
       ATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACC                                                                                         <  SRR4302540.1801202/94‑1 (MQ=60)
       ATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCA                                                                                        >  SRR4302540.548115/1‑94 (MQ=60)
        TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCA                                                                                        <  SRR4302540.8172053/94‑1 (MQ=60)
        TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCAT                                                                                       <  SRR4302540.9345532/94‑1 (MQ=60)
         CCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCAT                                                                                       <  SRR4302540.7111944/94‑1 (MQ=60)
         CCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCAT                                                                                       >  SRR4302540.1892572/1‑94 (MQ=60)
         CCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATC                                                                                      <  SRR4302540.4935648/94‑1 (MQ=60)
          CAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATC                                                                                      <  SRR4302540.509544/94‑1 (MQ=60)
           CGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCC                                                                                     <  SRR4302540.818350/94‑1 (MQ=60)
           AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATTGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCC                                                                                     <  SRR4302540.7656240/94‑1 (MQ=60)
           AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCC                                                                                     <  SRR4302540.8679758/94‑1 (MQ=60)
           AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCG                                                                                    <  SRR4302540.2376969/94‑1 (MQ=60)
            GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCG                                                                                    <  SRR4302540.4963324/94‑1 (MQ=60)
            GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCG                                                                                    <  SRR4302540.8503645/94‑1 (MQ=60)
            GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCG                                                                                    <  SRR4302540.7005010/94‑1 (MQ=60)
            GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGA                                                                                   >  SRR4302540.438022/1‑94 (MQ=60)
             ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGA                                                                                   <  SRR4302540.9615717/94‑1 (MQ=60)
               TGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCTCGTCTTGATCACCTCTACATACCATCCGAAGC                                                                                <  SRR4302540.8883112/94‑1 (MQ=60)
                 TTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCT                                                                               >  SRR4302540.412165/1‑94 (MQ=60)
                 TTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                                              <  SRR4302540.1250874/94‑1 (MQ=60)
                  TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCT                                                                               >  SRR4302540.5589053/1‑94 (MQ=60)
                   CTGAATGCGACGATAATTAAGGTTCTTTAATGGTTAGTTTTTTTATTGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            >  SRR4302540.1784414/1‑94 (MQ=60)
                   CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                                             >  SRR4302540.5845068/1‑94 (MQ=60)
                   CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                                             <  SRR4302540.3554229/94‑1 (MQ=60)
                   CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                                             >  SRR4302540.683346/1‑94 (MQ=60)
                   CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            >  SRR4302540.3891880/1‑94 (MQ=60)
                    TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            <  SRR4302540.3281267/94‑1 (MQ=60)
                    TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            >  SRR4302540.889936/1‑94 (MQ=60)
                    TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            <  SRR4302540.2698450/94‑1 (MQ=60)
                    TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTC                                                                           <  SRR4302540.3997106/94‑1 (MQ=60)
                      AATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGCAGCTTTTCT                                                                          >  SRR4302540.4033829/1‑94 (MQ=60)
                      AATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCT                                                                          <  SRR4302540.1551901/94‑1 (MQ=60)
                       ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                                         >  SRR4302540.4152682/1‑94 (MQ=60)
                        TGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTG                                                                        <  SRR4302540.9896640/94‑1 (MQ=60)
                          CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCG                                                                      <  SRR4302540.3536506/94‑1 (MQ=60)
                          CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCG                                                                      >  SRR4302540.9440721/1‑94 (MQ=60)
                          CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCG                                                                      >  SRR4302540.3356824/1‑94 (MQ=60)
                          CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑CACCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCG                                                                      >  SRR4302540.2113964/1‑94 (MQ=60)
                          CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGT                                                                     <  SRR4302540.515145/94‑1 (MQ=60)
                           GACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTG                                                                    <  SRR4302540.7366818/94‑1 (MQ=60)
                            ACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGT                                                                   <  SRR4302540.4429073/94‑1 (MQ=60)
                             CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATTACCTCTACATACCATCCGAAGCTTTTCTTGCGTGT                                                                   >  SRR4302540.3773617/1‑94 (MQ=60)
                             CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGT                                                                   <  SRR4302540.2077534/94‑1 (MQ=60)
                             CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGT                                                                   <  SRR4302540.7217643/94‑1 (MQ=60)
                             CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTT                                                                  >  SRR4302540.8771272/1‑94 (MQ=60)
                              GATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTT                                                                  >  SRR4302540.1063961/1‑94 (MQ=60)
                              GATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTC                                                                 <  SRR4302540.876309/94‑1 (MQ=60)
                              GATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAAAG‑‑CCGC‑CCGAGTCTTGATCAGGTCTACATAGCAGCCGAAGGTTTTATTGCGTGTT                                                                  >  SRR4302540.5113176/1‑94 (MQ=36)
                               ATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTC                                                                 >  SRR4302540.1707167/1‑94 (MQ=60)
                               ATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACAATCCGAAGTTTTTCTTGCGTGTTT                                                                 >  SRR4302540.7873931/1‑94 (MQ=60)
                               ATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                                                >  SRR4302540.3083788/1‑94 (MQ=60)
                               ATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                                                >  SRR4302540.2888885/1‑94 (MQ=60)
                                TAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                                                >  SRR4302540.9357376/1‑94 (MQ=60)
                                TAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTT                                                               <  SRR4302540.3493032/94‑1 (MQ=60)
                                TAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTT                                                               <  SRR4302540.5347166/94‑1 (MQ=60)
                                TAATTAAGGTGCTTTAATGGTTATTTTTTTTAAGGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                                                <  SRR4302540.9791739/94‑1 (MQ=60)
                                 AATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTT                                                               <  SRR4302540.7380731/94‑1 (MQ=60)
                                  ATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGT                                                             >  SRR4302540.10016195/1‑94 (MQ=60)
                                   TTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGT                                                             <  SRR4302540.3479363/94‑1 (MQ=60)
                                   TTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTG                                                            <  SRR4302540.2653009/94‑1 (MQ=60)
                                    TAAGGTGCTTTAATGGTTATTTTTTTTACTGACCGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGATTTTCTTGCGTGTACTTATG                                                            <  SRR4302540.9062116/94‑1 (MQ=60)
                                    TAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGA                                                           >  SRR4302540.2469462/1‑94 (MQ=60)
                                     AAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGG                                                           >  SRR4302540.8111761/1‑94 (MQ=60)
                                     AAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGA                                                           <  SRR4302540.689691/94‑1 (MQ=60)
                                     AAGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGA                                                           <  SRR4302540.4911749/94‑1 (MQ=60)
                                      AGGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          <  SRR4302540.7001481/94‑1 (MQ=60)
                                       GGTGCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGG                                                         >  SRR4302540.6401221/1‑94 (MQ=60)
                                         TGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                                      <  SRR4302540.3709002/94‑1 (MQ=60)
                                          GCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACGTCCCATCCGAAGCTGTTCTTGCGTGTTCTTGTGAGGCTT                                                      >  SRR4302540.9267175/1‑94 (MQ=60)
                                          GCTTTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                                      >  SRR4302540.7071361/1‑94 (MQ=60)
                                             TTAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCT                                                   <  SRR4302540.3699435/94‑1 (MQ=60)
                                              TAATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                                  >  SRR4302540.30030/1‑94 (MQ=60)
                                              TAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302540.5202377/94‑1 (MQ=60)
                                              TAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302540.6349670/94‑1 (MQ=60)
                                              TAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302540.1695096/94‑1 (MQ=60)
                                               AATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302540.8609846/94‑1 (MQ=60)
                                               AATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302540.573311/94‑1 (MQ=60)
                                               AATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 >  SRR4302540.5637050/1‑94 (MQ=60)
                                               AATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                                                <  SRR4302540.9564042/94‑1 (MQ=60)
                                                ATGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                                                <  SRR4302540.243987/94‑1 (MQ=60)
                                                 TGGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGC                                               >  SRR4302540.7274164/1‑94 (MQ=60)
                                                 TGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC                                              >  SRR4302540.6569044/1‑94 (MQ=60)
                                                 TGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC                                              >  SRR4302540.8729174/1‑94 (MQ=60)
                                                  GGTTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC                                              >  SRR4302540.6277472/1‑94 (MQ=60)
                                                   GTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAT                                            <  SRR4302540.8845686/94‑1 (MQ=60)
                                                    TTATTTTTTTTAATTACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAT                                            <  SRR4302540.3397418/94‑1 (MQ=60)
                                                    TTATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAT                                            <  SRR4302540.2707190/94‑1 (MQ=60)
                                                     TATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATT                                           <  SRR4302540.26880/94‑1 (MQ=60)
                                                     TATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATT                                           >  SRR4302540.3321991/1‑94 (MQ=60)
                                                     TATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTA                                          >  SRR4302540.10162912/1‑94 (MQ=60)
                                                      ATTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTA                                          <  SRR4302540.1113004/94‑1 (MQ=60)
                                                      ATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         <  SRR4302540.9154039/94‑1 (MQ=60)
                                                      ATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         <  SRR4302540.10104055/94‑1 (MQ=60)
                                                       TTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         >  SRR4302540.8632488/1‑94 (MQ=60)
                                                       TTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         <  SRR4302540.7143801/94‑1 (MQ=60)
                                                       TTTTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         >  SRR4302540.6392280/1‑94 (MQ=60)
                                                       TTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATC                                        <  SRR4302540.5862001/94‑1 (MQ=60)
                                                         TTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                                       <  SRR4302540.1739794/94‑1 (MQ=60)
                                                         TTTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                                       <  SRR4302540.6149279/94‑1 (MQ=60)
                                                         TTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCAT                                      <  SRR4302540.9162121/94‑1 (MQ=60)
                                                         TTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCAT                                      <  SRR4302540.9370132/94‑1 (MQ=60)
                                                         TTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCAT                                      <  SRR4302540.9974114/94‑1 (MQ=60)
                                                          TTTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCCCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCAT                                      <  SRR4302540.4981581/94‑1 (MQ=60)
                                                          TGTTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCAT                                      <  SRR4302540.4500481/94‑1 (MQ=60)
                                                           TTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302540.802737/94‑1 (MQ=60)
                                                           TTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302540.8514859/94‑1 (MQ=60)
                                                            TTTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    >  SRR4302540.8979064/1‑94 (MQ=60)
                                                            TTTAATGACAGC‑CC‑C‑CCGCGTCTGGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302540.3981633/94‑1 (MQ=60)
                                                            TTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   >  SRR4302540.983266/1‑94 (MQ=60)
                                                            TTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302540.6037969/94‑1 (MQ=60)
                                                            TTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   >  SRR4302540.805719/1‑94 (MQ=60)
                                                            TTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302540.4209872/94‑1 (MQ=60)
                                                             TTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302540.9681412/94‑1 (MQ=60)
                                                             TTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302540.2193717/94‑1 (MQ=60)
                                                             TTAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   >  SRR4302540.7790343/1‑94 (MQ=60)
                                                              TAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                                  <  SRR4302540.8429047/94‑1 (MQ=60)
                                                              TAATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                                  <  SRR4302540.3332480/94‑1 (MQ=60)
                                                              TAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGA                                 <  SRR4302540.2592984/94‑1 (MQ=60)
                                                               AATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGA                                 >  SRR4302540.6927755/1‑94 (MQ=60)
                                                                ATGACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAT                                >  SRR4302540.5287276/1‑94 (MQ=60)
                                                                  GACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCC                              <  SRR4302540.7968683/94‑1 (MQ=60)
                                                                  GACAGC‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCC                              <  SRR4302540.763051/94‑1 (MQ=60)
                                                                   ACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCAC                            >  SRR4302540.1183429/1‑94 (MQ=60)
                                                                      agcCC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA                           <  SRR4302540.1727317/91‑1 (MQ=60)
                                                                      agcCC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA                           >  SRR4302540.3840383/4‑94 (MQ=60)
                                                                      agcCC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA                           >  SRR4302540.5219960/4‑94 (MQ=60)
                                                                      C‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         >  SRR4302540.1623965/1‑94 (MQ=60)
                                                                      C‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         <  SRR4302540.9592329/94‑1 (MQ=60)
                                                                      C‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         >  SRR4302540.1901124/1‑94 (MQ=60)
                                                                      C‑‑CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         >  SRR4302540.3180556/1‑94 (MQ=60)
                                                                       gcCC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACAT                          <  SRR4302540.9229186/92‑1 (MQ=60)
                                                                         CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA                        <  SRR4302540.2253252/94‑1 (MQ=60)
                                                                         CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA                        >  SRR4302540.158144/1‑94 (MQ=60)
                                                                         CC‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA                        >  SRR4302540.5925655/1‑94 (MQ=60)
                                                                          C‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAA                       <  SRR4302540.6645617/94‑1 (MQ=60)
                                                                          C‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAA                       >  SRR4302540.4757827/1‑94 (MQ=60)
                                                                          C‑C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGACCCACATAAA                       >  SRR4302540.1330615/1‑94 (MQ=60)
                                                                            C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      >  SRR4302540.4996747/1‑94 (MQ=60)
                                                                            C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      >  SRR4302540.9480326/1‑94 (MQ=60)
                                                                            C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      >  SRR4302540.8752714/1‑94 (MQ=60)
                                                                            C‑CCGCGTCTTGATCACCTCTACATACCATCCGAAGCCTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCGTTATCATCACGATCCACATAAAT                      >  SRR4302540.1371987/1‑94 (MQ=60)
                                                                              CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA                     <  SRR4302540.3830182/94‑1 (MQ=60)
                                                                               CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    <  SRR4302540.6232426/94‑1 (MQ=60)
                                                                               CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    <  SRR4302540.6803190/94‑1 (MQ=60)
                                                                               CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    <  SRR4302540.9175040/94‑1 (MQ=60)
                                                                               CGCGGCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    >  SRR4302540.3034420/1‑94 (MQ=60)
                                                                                GCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAA                   <  SRR4302540.209324/94‑1 (MQ=60)
                                                                                GCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAA                   <  SRR4302540.6450226/94‑1 (MQ=60)
                                                                                GCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAA                   >  SRR4302540.2788279/1‑94 (MQ=60)
                                                                                  GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 >  SRR4302540.8313645/1‑94 (MQ=60)
                                                                                  GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 <  SRR4302540.3435098/94‑1 (MQ=60)
                                                                                  GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 <  SRR4302540.2671816/94‑1 (MQ=60)
                                                                                   TCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCC                <  SRR4302540.8158906/94‑1 (MQ=60)
                                                                                   GCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCC                <  SRR4302540.5665294/94‑1 (MQ=60)
                                                                                    CTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               <  SRR4302540.3503701/94‑1 (MQ=60)
                                                                                    CTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               <  SRR4302540.7908051/94‑1 (MQ=60)
                                                                                     TTGATCCCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGT              <  SRR4302540.931666/94‑1 (MQ=60)
                                                                                     TTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGT              >  SRR4302540.4655990/1‑94 (MQ=60)
                                                                                      TGATCTCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTA             <  SRR4302540.5457842/94‑1 (MQ=60)
                                                                                      TGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTA             <  SRR4302540.6153095/94‑1 (MQ=60)
                                                                                       TATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302540.2964947/94‑1 (MQ=60)
                                                                                       GATCCCCTCTACATCCCATCCGAAGGTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302540.2538940/94‑1 (MQ=60)
                                                                                        ATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGC           >  SRR4302540.1050347/1‑94 (MQ=60)
                                                                                        ATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAATATCATCACGATCCACATAAATAAAGCCGTAGC           >  SRR4302540.9821535/1‑94 (MQ=60)
                                                                                         TCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCG          <  SRR4302540.5230628/94‑1 (MQ=60)
                                                                                         TCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCG          <  SRR4302540.3897791/94‑1 (MQ=60)
                                                                                          CACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGC         <  SRR4302540.5764059/94‑1 (MQ=60)
                                                                                          CACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGC         >  SRR4302540.1501553/1‑94 (MQ=60)
                                                                                          CACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGC         >  SRR4302540.6083732/1‑94 (MQ=60)
                                                                                            CCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTGGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTT       >  SRR4302540.1208258/1‑94 (MQ=60)
                                                                                             CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      >  SRR4302540.9780783/1‑94 (MQ=60)
                                                                                             CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      >  SRR4302540.1329649/1‑94 (MQ=60)
                                                                                             CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      >  SRR4302540.7317554/1‑94 (MQ=60)
                                                                                              TCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAG     <  SRR4302540.4508799/94‑1 (MQ=60)
                                                                                               CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    >  SRR4302540.156221/1‑94 (MQ=60)
                                                                                               CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    <  SRR4302540.3956849/94‑1 (MQ=60)
                                                                                               CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    <  SRR4302540.2012798/94‑1 (MQ=60)
                                                                                               CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    <  SRR4302540.5534139/94‑1 (MQ=60)
                                                                                                TACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGAC   <  SRR4302540.2189041/94‑1 (MQ=60)
                                                                                                TACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGAC   >  SRR4302540.3299288/1‑94 (MQ=60)
                                                                                                 ACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCTCCATTATCATTACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  SRR4302540.9042842/1‑94 (MQ=60)
                                                                                                 ACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  <  SRR4302540.5889028/94‑1 (MQ=60)
                                                                                                 ACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  <  SRR4302540.7476155/94‑1 (MQ=60)
                                                                                                 |                                                                                             
GCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑‑CC‑C‑CCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  NC_000913/3899882‑3900068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑