Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,975 G→A A452V (GCA→GTA)  bglB ← phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,9750GA100.0% 953.0 / NA 258A452V (GCA→GTA) bglBphospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (111/147);  total (111/147)

Only 100 of 265 total aligned reads displayed.

CGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑G‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3899886‑3900064
                                                                                            |                                                                                         
   atcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATAc                                                                                      >  2:2743827/1‑93 (MQ=255)
   atcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATAc                                                                                      >  2:4664455/1‑93 (MQ=255)
     ccAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCa                                                                                    >  1:6140671/1‑93 (MQ=255)
     ccAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCa                                                                                    <  1:5490127/93‑1 (MQ=255)
     ccAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCa                                                                                    >  1:652232/1‑93 (MQ=255)
     ccAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCa                                                                                    >  2:4282232/1‑93 (MQ=255)
       aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                                                  <  2:2471539/93‑1 (MQ=255)
       aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                                                  <  2:3033171/93‑1 (MQ=255)
       aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                                                  <  2:322201/93‑1 (MQ=255)
         aCTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCg                                                                                <  1:4195586/93‑1 (MQ=255)
          cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                                               <  1:5993986/93‑1 (MQ=255)
          cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                                               <  1:4507710/93‑1 (MQ=255)
          cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                                               <  1:3772702/93‑1 (MQ=255)
           tGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGaa                                                                              <  1:1099620/93‑1 (MQ=255)
             ttCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGc                                                                            <  2:4132154/93‑1 (MQ=255)
              tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCt                                                                           <  1:1032921/93‑1 (MQ=255)
               cTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCCCCTCTACATGCCATCCGAAGCtt                                                                          <  1:3458301/93‑1 (MQ=255)
               cTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCtt                                                                          >  1:1885149/1‑93 (MQ=255)
                tGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCttt                                                                         <  2:4527366/93‑1 (MQ=255)
                 gAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCtttt                                                                        <  1:5895369/93‑1 (MQ=255)
                 gAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCtttt                                                                        <  1:5174413/93‑1 (MQ=255)
                  aaTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTc                                                                       >  2:1491303/1‑93 (MQ=255)
                   aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACGT‑C‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                                                      <  2:500107/93‑1 (MQ=255)
                   aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                                                      <  1:1420408/93‑1 (MQ=255)
                   aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                                                      <  1:490728/93‑1 (MQ=255)
                   aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTGAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                                                      <  2:3818483/93‑1 (MQ=255)
                    tGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCtt                                                                     <  2:3934260/93‑1 (MQ=255)
                    tGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCtt                                                                     >  2:5867235/1‑93 (MQ=255)
                      cgacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGc                                                                   <  2:3672332/93‑1 (MQ=255)
                      cgacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTACTTGc                                                                   <  1:5643584/93‑1 (MQ=255)
                       gacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCg                                                                  <  2:1034197/93‑1 (MQ=255)
                          gaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCgtgt                                                               >  2:255303/1‑93 (MQ=255)
                          gaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGGCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTCCgtgt                                                               >  1:4096154/1‑93 (MQ=255)
                              aTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCtt                                                           <  1:4748764/93‑1 (MQ=255)
                              aTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCtt                                                           >  1:2059434/1‑93 (MQ=255)
                               ttAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                                          <  1:6202491/93‑1 (MQ=255)
                                tAAGGTTGTTTAATGGTTTTTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtgt                                                         <  2:5602877/93‑1 (MQ=255)
                                tAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtgt                                                         <  2:1375607/93‑1 (MQ=255)
                                  aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  2:1194330/93‑1 (MQ=255)
                                  aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  1:1166974/93‑1 (MQ=255)
                                  aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                                                       <  2:1757665/93‑1 (MQ=255)
                                   ggTGCTTTAATGGTTATTTTTTTTAATGAC‑AGG‑CCCCCGCGTCCTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGGTCTTTTGAg                                                      >  2:4829121/1‑93 (MQ=255)
                                   ggTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAg                                                      <  2:2329583/93‑1 (MQ=255)
                                   agggCTTTTAAGGGTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAg                                                      <  2:1907876/90‑1 (MQ=255)
                                      gCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                                                   <  2:751267/93‑1 (MQ=255)
                                      gCTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                                                   >  1:5157260/1‑93 (MQ=255)
                                       cTTTAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCCTGGTGACCTCCTGATACCATCCGGAGCTCTTTTTGCGTGTTCTTGTggggtgt                                                  >  2:2196320/1‑90 (MQ=255)
                                        tttAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                                                 >  1:3294696/1‑93 (MQ=255)
                                         ttAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcc                                                >  1:4858943/1‑93 (MQ=255)
                                          tAATGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcct                                               <  2:4497235/93‑1 (MQ=255)
                                           aaTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              <  2:2201849/93‑1 (MQ=255)
                                           aaTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              <  1:2567652/93‑1 (MQ=255)
                                           aaTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              <  1:942263/93‑1 (MQ=255)
                                           aaTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              >  1:5462941/1‑93 (MQ=255)
                                            aTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttccttc                                             >  2:5454129/1‑93 (MQ=255)
                                            aTGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttccttc                                             <  1:5341690/93‑1 (MQ=255)
                                             tGGTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCg                                            >  1:5198732/1‑93 (MQ=255)
                                               gTTATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGcc                                          >  2:603343/1‑93 (MQ=255)
                                                ttATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                                         >  1:2230325/1‑93 (MQ=255)
                                                 tATTTTTTTTAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAt                                        <  2:4896629/93‑1 (MQ=255)
                                                   ttttttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCCTTa                                      >  2:5194043/1‑93 (MQ=255)
                                                   ttttttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa                                      <  1:5760034/93‑1 (MQ=255)
                                                    tttttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTat                                     <  1:3122026/93‑1 (MQ=255)
                                                     ttttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatc                                    <  1:3938338/93‑1 (MQ=255)
                                                      tttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca                                   >  2:5357175/1‑93 (MQ=255)
                                                      tttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca                                   >  1:2539142/1‑93 (MQ=255)
                                                      tttttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca                                   <  2:2001208/93‑1 (MQ=255)
                                                         ttAATGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca                                >  2:2463133/1‑93 (MQ=255)
                                                          tAATGAC‑A‑GCCCCCCGCGGCTTGAGCACCTCTACATACCATCCGAAGGTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCCATATCAGCAc                               >  2:3526380/1‑93 (MQ=255)
                                                            aTGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGa                             >  2:2860093/1‑93 (MQ=255)
                                                            aTGAC‑A‑GCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGa                             <  1:2908852/93‑1 (MQ=255)
                                                                   agccccccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        >  2:1678051/4‑93 (MQ=255)
                                                                   agccccccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        <  1:4690595/90‑1 (MQ=255)
                                                                    gccccccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcaca                       <  2:4465608/91‑1 (MQ=255)
                                                                      cccccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACata                     >  1:4867964/1‑93 (MQ=255)
                                                                       ccccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataa                    >  1:2871498/1‑93 (MQ=255)
                                                                        cccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaa                   >  2:6233125/1‑93 (MQ=255)
                                                                        cccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaa                   <  2:1315518/93‑1 (MQ=255)
                                                                        cccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaa                   <  1:3317171/93‑1 (MQ=255)
                                                                         ccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaat                  >  2:211924/1‑93 (MQ=255)
                                                                         ccGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaat                  >  1:5268945/1‑93 (MQ=255)
                                                                           gcgTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaataa                >  1:4280113/1‑93 (MQ=255)
                                                                           gcgTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACataaataa                <  1:1266899/93‑1 (MQ=255)
                                                                            cgTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACAtaaataaa               <  1:828356/93‑1 (MQ=255)
                                                                              tCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             >  1:2708608/1‑93 (MQ=255)
                                                                              tCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             <  1:3868446/93‑1 (MQ=255)
                                                                              tCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGc             <  1:4177405/93‑1 (MQ=255)
                                                                               tttGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGcc            <  1:1453987/92‑1 (MQ=255)
                                                                                  taTCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         <  1:1504504/92‑1 (MQ=255)
                                                                                  gATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         >  1:3690512/1‑93 (MQ=255)
                                                                                  gATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         >  1:172314/1‑93 (MQ=255)
                                                                                  gATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         <  2:4844778/93‑1 (MQ=255)
                                                                                  gATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa         <  1:5507867/93‑1 (MQ=255)
                                                                                    tCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAgc       >  2:5864761/1‑93 (MQ=255)
                                                                                    tCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAgc       >  2:3000247/1‑93 (MQ=255)
                                                                                    tCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAgc       >  2:265843/1‑93 (MQ=255)
                                                                                      aCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAgcgc     >  2:5662587/1‑93 (MQ=255)
                                                                                      aCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAgcgc     >  2:1780209/1‑93 (MQ=255)
                                                                                       ccTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCt    <  2:3756091/93‑1 (MQ=255)
                                                                                         tctACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTa  <  2:702538/93‑1 (MQ=255)
                                                                                            |                                                                                         
CGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑A‑G‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3899886‑3900064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  NC_000913/3899883‑3900068
                                                                                             |                                                                                             
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                                               <  SRR4302533.2684249/94‑1 (MQ=60)
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                                               >  SRR4302533.4618477/1‑94 (MQ=60)
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                                               >  SRR4302533.3011269/1‑94 (MQ=60)
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                                               <  SRR4302533.5653528/94‑1 (MQ=60)
 ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTAC                                                                                              >  SRR4302533.915450/1‑94 (MQ=60)
  TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACA                                                                                             >  SRR4302533.2250961/1‑94 (MQ=60)
  TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACA                                                                                             <  SRR4302533.1346170/94‑1 (MQ=60)
    GCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATA                                                                                           >  SRR4302533.144975/1‑94 (MQ=60)
       TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCA                                                                                        >  SRR4302533.6177656/1‑94 (MQ=60)
       TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCA                                                                                        >  SRR4302533.656084/1‑94 (MQ=60)
        CCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCAT                                                                                       <  SRR4302533.5523223/94‑1 (MQ=60)
          AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCC                                                                                     <  SRR4302533.146685/94‑1 (MQ=60)
          AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCC                                                                                     <  SRR4302533.1888099/94‑1 (MQ=60)
           GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCG                                                                                    <  SRR4302533.4297359/94‑1 (MQ=60)
            ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                                   >  SRR4302533.4002502/1‑94 (MQ=60)
            ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                                   >  SRR4302533.1864459/1‑94 (MQ=60)
            ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                                   <  SRR4302533.4220983/94‑1 (MQ=60)
             CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                                  <  SRR4302533.4535010/94‑1 (MQ=60)
             CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                                  >  SRR4302533.5941966/1‑94 (MQ=60)
             CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                                  <  SRR4302533.3795828/94‑1 (MQ=60)
             CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                                  <  SRR4302533.6030289/94‑1 (MQ=60)
              TGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAG                                                                                 <  SRR4302533.5503012/94‑1 (MQ=60)
              TGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAG                                                                                 <  SRR4302533.1106138/94‑1 (MQ=60)
                 TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                                              <  SRR4302533.1038994/94‑1 (MQ=60)
                 TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                                              <  SRR4302533.1547843/94‑1 (MQ=60)
                 TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                                              >  SRR4302533.1896662/1‑94 (MQ=60)
                  CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCCCCTCTACATGCCATCCGAAGCTTT                                                                             <  SRR4302533.3479650/94‑1 (MQ=60)
                  CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                                             <  SRR4302533.4725150/94‑1 (MQ=60)
                  CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                                             <  SRR4302533.1433234/94‑1 (MQ=60)
                   TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            <  SRR4302533.5199480/94‑1 (MQ=60)
                   TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                                            <  SRR4302533.1359272/94‑1 (MQ=60)
                   TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATAACATCCGAAGCTTTT                                                                            >  SRR4302533.5366467/1‑94 (MQ=60)
                   TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGGCTTGTTCACCTCTACATACCATACGAAAGTTTT                                                                            >  SRR4302533.3328628/1‑94 (MQ=60)
                    GAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTC                                                                           <  SRR4302533.5930971/94‑1 (MQ=60)
                    GAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTC                                                                           <  SRR4302533.5205669/94‑1 (MQ=60)
                     AATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCT                                                                          <  SRR4302533.2913222/94‑1 (MQ=60)
                      ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                                         <  SRR4302533.493662/94‑1 (MQ=60)
                      ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                                         <  SRR4302533.3894862/94‑1 (MQ=60)
                      ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                                         <  SRR4302533.6296977/94‑1 (MQ=60)
                      ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                                         <  SRR4302533.1429002/94‑1 (MQ=60)
                         CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTACTTGCG                                                                      <  SRR4302533.5677699/94‑1 (MQ=60)
                            CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGGCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTCCGTGT                                                                   >  SRR4302533.4121090/1‑94 (MQ=60)
                             GATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTT                                                                  >  SRR4302533.3205397/1‑94 (MQ=60)
                               TAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                                                >  SRR4302533.2392756/1‑94 (MQ=60)
                                AATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTT                                                               >  SRR4302533.2072132/1‑94 (MQ=60)
                                 ATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTG                                                              <  SRR4302533.4777556/94‑1 (MQ=60)
                                  TTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGT                                                             <  SRR4302533.6239690/94‑1 (MQ=60)
                                     AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          >  SRR4302533.995344/1‑94 (MQ=60)
                                     AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          <  SRR4302533.1173977/94‑1 (MQ=60)
                                     AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          <  SRR4302533.5437729/94‑1 (MQ=60)
                                     AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          <  SRR4302533.2598732/94‑1 (MQ=60)
                                     AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                                          <  SRR4302533.5278740/94‑1 (MQ=60)
                                        TGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCT                                                       >  SRR4302533.5188396/1‑94 (MQ=60)
                                         gcgtttaagtttttaTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                                      <  SRR4302533.6304313/79‑1 (MQ=60)
                                         GCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                                      <  SRR4302533.1334652/94‑1 (MQ=60)
                                          CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                                     >  SRR4302533.3315030/1‑94 (MQ=60)
                                          CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                                     >  SRR4302533.6214009/1‑94 (MQ=60)
                                          CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                                     <  SRR4302533.314946/94‑1 (MQ=60)
                                          CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                                     >  SRR4302533.1925123/1‑94 (MQ=60)
                                          CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                                     <  SRR4302533.2571920/94‑1 (MQ=60)
                                           TTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCC                                                    >  SRR4302533.4888394/1‑94 (MQ=60)
                                            TTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCT                                                   <  SRR4302533.2724748/94‑1 (MQ=60)
                                            TTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCT                                                   <  SRR4302533.598123/94‑1 (MQ=60)
                                             TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                                  <  SRR4302533.4166900/94‑1 (MQ=60)
                                             TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                                  >  SRR4302533.5659495/1‑94 (MQ=60)
                                             TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                                  >  SRR4302533.3058949/1‑94 (MQ=60)
                                             TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                                  >  SRR4302533.5495855/1‑94 (MQ=60)
                                              AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302533.4674150/94‑1 (MQ=60)
                                              AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302533.2583454/94‑1 (MQ=60)
                                              AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302533.947805/94‑1 (MQ=60)
                                              AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 >  SRR4302533.114205/1‑94 (MQ=60)
                                              AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                                                 <  SRR4302533.43297/94‑1 (MQ=60)
                                               ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                                                >  SRR4302533.5230080/1‑94 (MQ=60)
                                               ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                                                <  SRR4302533.5373724/94‑1 (MQ=60)
                                               ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCC                                                >  SRR4302533.5214297/1‑94 (MQ=60)
                                                 TGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC                                              <  SRR4302533.5176758/94‑1 (MQ=60)
                                                  GTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCA                                             <  SRR4302533.5205689/94‑1 (MQ=60)
                                                  GTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCA                                             >  SRR4302533.2244088/1‑94 (MQ=60)
                                                     ATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTA                                          <  SRR4302533.4206536/94‑1 (MQ=60)
                                                      TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         <  SRR4302533.5794791/94‑1 (MQ=60)
                                                      TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                                         <  SRR4302533.2169329/94‑1 (MQ=60)
                                                       TTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATC                                        <  SRR4302533.3141221/94‑1 (MQ=60)
                                                        TTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                                       >  SRR4302533.2554774/1‑94 (MQ=60)
                                                        TTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                                       <  SRR4302533.3962377/94‑1 (MQ=60)
                                                          TTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATC                                     <  SRR4302533.5240697/94‑1 (MQ=60)
                                                          TTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATC                                     >  SRR4302533.1564321/1‑94 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCGCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302533.6324957/94‑1 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGGTTTCTTCGCCATTATTATCA                                    >  SRR4302533.3358235/1‑94 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    >  SRR4302533.1088577/1‑94 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302533.2737073/94‑1 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302533.5705038/94‑1 (MQ=60)
                                                           TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                                    <  SRR4302533.761655/94‑1 (MQ=60)
                                                            TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302533.6238434/94‑1 (MQ=60)
                                                            TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   >  SRR4302533.3849379/1‑94 (MQ=60)
                                                            TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                                   <  SRR4302533.1173282/94‑1 (MQ=60)
                                                             TAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                                  >  SRR4302533.3981422/1‑94 (MQ=60)
                                                             TAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                                  <  SRR4302533.3030833/94‑1 (MQ=60)
                                                              AATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGA                                 >  SRR4302533.5844000/1‑94 (MQ=60)
                                                               ATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAT                                <  SRR4302533.2926708/94‑1 (MQ=60)
                                                                 GACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCC                              >  SRR4302533.5120072/1‑94 (MQ=60)
                                                                    agcCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA                           >  SRR4302533.2725306/4‑94 (MQ=60)
                                                                    agcCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA                           <  SRR4302533.4719028/91‑1 (MQ=60)
                                                                     C‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         >  SRR4302533.4897463/1‑94 (MQ=60)
                                                                     C‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA                         <  SRR4302533.3737335/94‑1 (MQ=60)
                                                                       CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA                        >  SRR4302533.2889166/1‑94 (MQ=60)
                                                                       CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA                        >  SRR4302533.387195/1‑94 (MQ=60)
                                                                         CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      >  SRR4302533.5300550/1‑94 (MQ=60)
                                                                         CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      <  SRR4302533.3337656/94‑1 (MQ=60)
                                                                         CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      <  SRR4302533.4274685/94‑1 (MQ=60)
                                                                         CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT                      >  SRR4302533.4407883/1‑94 (MQ=60)
                                                                          CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA                     >  SRR4302533.4916913/1‑94 (MQ=60)
                                                                          CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA                     >  SRR4302533.3437780/1‑94 (MQ=60)
                                                                          CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA                     <  SRR4302533.484585/94‑1 (MQ=60)
                                                                          CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA                     >  SRR4302533.5380994/1‑94 (MQ=60)
                                                                           CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    <  SRR4302533.5522430/94‑1 (MQ=60)
                                                                           CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA                    >  SRR4302533.4305846/1‑94 (MQ=60)
                                                                            GCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAA                   <  SRR4302533.1274478/94‑1 (MQ=60)
                                                                             CGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAG                  >  SRR4302533.474230/1‑94 (MQ=60)
                                                                             CGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAG                  <  SRR4302533.833213/94‑1 (MQ=60)
                                                                              GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 >  SRR4302533.6152722/1‑94 (MQ=60)
                                                                              GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 >  SRR4302533.4894018/1‑94 (MQ=60)
                                                                              GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 >  SRR4302533.2725229/1‑94 (MQ=60)
                                                                              GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 >  SRR4302533.5572415/1‑94 (MQ=60)
                                                                              GTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGC                 <  SRR4302533.879788/94‑1 (MQ=60)
                                                                               TCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCC                <  SRR4302533.4912999/94‑1 (MQ=60)
                                                                               TCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCC                <  SRR4302533.3892065/94‑1 (MQ=60)
                                                                               TCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCC                <  SRR4302533.4202715/94‑1 (MQ=60)
                                                                                TTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               <  SRR4302533.1462756/94‑1 (MQ=60)
                                                                                CTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               >  SRR4302533.1258891/1‑94 (MQ=60)
                                                                                CTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               <  SRR4302533.2239881/94‑1 (MQ=60)
                                                                                CTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCG               <  SRR4302533.4184227/94‑1 (MQ=60)
                                                                                  TGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTA             >  SRR4302533.5841833/1‑94 (MQ=60)
                                                                                  TGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTA             >  SRR4302533.173402/1‑94 (MQ=60)
                                                                                  TGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTA             >  SRR4302533.3713186/1‑94 (MQ=60)
                                                                                   TATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302533.1513575/94‑1 (MQ=60)
                                                                                   GATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302533.4850306/94‑1 (MQ=60)
                                                                                   GATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302533.5541111/94‑1 (MQ=60)
                                                                                   GATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAG            <  SRR4302533.5541110/94‑1 (MQ=60)
                                                                                     TCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCG          >  SRR4302533.5183260/1‑94 (MQ=60)
                                                                                         CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      <  SRR4302533.884646/94‑1 (MQ=60)
                                                                                         CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      >  SRR4302533.3591849/1‑94 (MQ=60)
                                                                                         CTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA      >  SRR4302533.3530097/1‑94 (MQ=60)
                                                                                           CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    >  SRR4302533.6063045/1‑94 (MQ=60)
                                                                                           CTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGA    >  SRR4302533.183641/1‑94 (MQ=60)
                                                                                            TACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGAC   <  SRR4302533.2663465/94‑1 (MQ=60)
                                                                                             ACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  SRR4302533.4728564/1‑94 (MQ=60)
                                                                                             ACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  <  SRR4302533.1177875/94‑1 (MQ=60)
                                                                                             |                                                                                             
CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACA  >  NC_000913/3899883‑3900068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑