Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,918,274 T→C K81K (AAA→AAG)  atpF ← membrane‑bound ATP synthase, F0 sector, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,2740TC99.8% 1642.1 / ‑5.7 507K81K (AAA→AAG) atpFmembrane‑bound ATP synthase, F0 sector, subunit b
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (288/217);  total (290/217)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 530 total aligned reads displayed.

GGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT  >  NC_000913/3918184‑3918363
                                                                                          |                                                                                         
ggCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGctt                                                                                         >  1:4662420/1‑93 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        <  1:6833559/93‑1 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        <  1:7537787/93‑1 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        >  2:7631175/1‑93 (MQ=255)
   ttCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGtt                                                                                      <  1:491549/93‑1 (MQ=255)
   ttCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGtt                                                                                      >  2:6199220/1‑93 (MQ=255)
    tCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTc                                                                                     >  1:99920/1‑93 (MQ=255)
    tCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTc                                                                                     >  2:610030/1‑93 (MQ=255)
     cAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCg                                                                                    >  1:5806957/1‑93 (MQ=255)
       aTTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGcc                                                                                  >  2:5389605/1‑93 (MQ=255)
       aTTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGcc                                                                                  <  2:4928030/93‑1 (MQ=255)
        tttCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCt                                                                                 >  1:6169926/1‑93 (MQ=255)
        tttCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCt                                                                                 >  1:780610/1‑93 (MQ=255)
          tCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGc                                                                               >  1:8062830/1‑93 (MQ=255)
          tCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGc                                                                               >  1:4177824/1‑93 (MQ=255)
              cctgcgcctgGGACACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTCCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCga                                                                           <  2:4289435/93‑1 (MQ=255)
               ctgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgag                                                                          >  1:4325676/1‑92 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatg                                                                         >  2:9129200/1‑93 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatg                                                                         >  1:721579/1‑93 (MQ=255)
                 gcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatga                                                                        <  1:1594918/93‑1 (MQ=255)
                  cgcgtgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatgat                                                                       <  1:3698999/93‑1 (MQ=255)
                    cctgGGCCACGATTTTAGTTCGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTa                                                                     <  1:2421729/93‑1 (MQ=255)
                     ctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAc                                                                    >  2:3235650/1‑93 (MQ=255)
                        ggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTg                                                                 <  1:2159883/93‑1 (MQ=255)
                        ggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTg                                                                 >  1:9834063/1‑93 (MQ=255)
                        ggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTg                                                                 <  2:306915/93‑1 (MQ=255)
                        ggCCACGATTTTAGTACGTGCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTg                                                                 <  1:7017070/93‑1 (MQ=255)
                         gCCTCGATTTTAGTTCGTTCCTGCTGTCCCTCAGCTTTCGCTGCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTgg                                                                <  2:5547472/93‑1 (MQ=255)
                         gCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTgg                                                                >  2:5742860/1‑93 (MQ=255)
                         gCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTgg                                                                >  2:8453072/1‑93 (MQ=255)
                            aCGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCt                                                             >  1:5878249/1‑93 (MQ=255)
                              gATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTc                                                           <  2:6699558/93‑1 (MQ=255)
                               aTTTTAGTACGTTATTGTTCTGCCTCAGCTTTCGCTTCGTGCAGAATCTGCGAGCGGCGCTTGTTTGCCTGCTCGATGATTACCTGGGCTTcc                                                          >  2:6242927/1‑93 (MQ=255)
                                ttttAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcg                                                         >  1:791454/1‑93 (MQ=255)
                                  ttAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgct                                                       >  2:7922738/1‑93 (MQ=255)
                                    aGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgcttt                                                     <  2:1725543/93‑1 (MQ=255)
                                     gTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttc                                                    <  2:3727741/93‑1 (MQ=255)
                                     gTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttc                                                    <  2:1534922/93‑1 (MQ=255)
                                      tACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcg                                                   >  2:3363575/1‑93 (MQ=255)
                                        cGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgct                                                 >  2:4690749/1‑93 (MQ=255)
                                        cGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgct                                                 >  1:1852997/1‑93 (MQ=255)
                                          ttCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgcttt                                               <  2:643901/93‑1 (MQ=255)
                                           tCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCtttt                                              >  1:1386732/1‑93 (MQ=255)
                                            ccTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCttttt                                             >  2:722200/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCATAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           >  2:2087559/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           >  2:1827565/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           >  1:4546098/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           >  1:849496/1‑93 (MQ=255)
                                               gTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGGGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCa                                          >  1:2892763/1‑93 (MQ=255)
                                                ttCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAg                                         >  2:7527503/1‑93 (MQ=255)
                                                ttCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAg                                         >  2:5974639/1‑93 (MQ=255)
                                                ttCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGACTGCTCGATGATTACCTAGGCTTCCGCTTTCGCTTTTTTTAg                                         >  2:8653707/1‑93 (MQ=255)
                                                  cTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCt                                       >  1:5376229/1‑93 (MQ=255)
                                                   tGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTg                                      >  2:1326550/1‑93 (MQ=255)
                                                   tGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTg                                      <  2:3886718/93‑1 (MQ=255)
                                                    gCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTgg                                     >  1:3198040/1‑93 (MQ=255)
                                                    gCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTgg                                     >  2:41786/1‑93 (MQ=255)
                                                    gCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTgg                                     >  2:2841451/1‑93 (MQ=255)
                                                     ccgcAGCTTTCGCTTCGCCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggt                                    <  1:2080039/90‑1 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCGTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   <  1:6329947/93‑1 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   <  2:1123909/93‑1 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   >  2:192685/1‑93 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  >  1:920834/1‑93 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  <  1:4790275/93‑1 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  >  1:893571/1‑93 (MQ=255)
                                                          gCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcggtc                               <  1:3551347/93‑1 (MQ=255)
                                                          gCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcggtc                               >  1:10010398/1‑93 (MQ=255)
                                                          gCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcggtc                               <  1:1124924/93‑1 (MQ=255)
                                                            tttCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCTATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTcgc                             <  2:8808195/93‑1 (MQ=255)
                                                            tttCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCTGCTggtcggtctc                             >  2:6945531/1‑91 (MQ=255)
                                                             ttCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTcgcg                            >  2:950183/1‑93 (MQ=255)
                                                              tCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCgcgc                           >  2:9321929/1‑93 (MQ=255)
                                                               cGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCt                          <  1:6585696/93‑1 (MQ=255)
                                                                gCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTg                         >  2:58556/1‑93 (MQ=255)
                                                                  ttCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGc                       >  1:1056991/1‑93 (MQ=255)
                                                                   tCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGcc                      >  2:9067621/1‑93 (MQ=255)
                                                                      tCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCttt                   >  2:8929338/1‑93 (MQ=255)
                                                                        cAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGTTGGCCtttac                 >  1:8130370/1‑91 (MQ=255)
                                                                        cAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGc                 >  1:8230879/1‑93 (MQ=255)
                                                                          gAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaa               >  2:5881437/1‑93 (MQ=255)
                                                                          gAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaa               <  1:5230956/93‑1 (MQ=255)
                                                                          gAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaa               <  2:6779651/93‑1 (MQ=255)
                                                                          gAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaa               <  2:9599261/93‑1 (MQ=255)
                                                                           aaTCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaag              >  1:8609218/1‑93 (MQ=255)
                                                                              cTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtc           <  1:3407205/93‑1 (MQ=255)
                                                                               tGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtca          >  1:9745545/1‑93 (MQ=255)
                                                                                 cGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaag        <  2:7841787/93‑1 (MQ=255)
                                                                                 cGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaag        <  1:5583985/93‑1 (MQ=255)
                                                                                  gAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaagg       >  1:8753501/1‑93 (MQ=255)
                                                                                    gcggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCaaggtcaaggtc     >  2:8141694/1‑93 (MQ=255)
                                                                                    gcggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCaaggtcaaggtc     <  1:182085/93‑1 (MQ=255)
                                                                                     cggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTcc    >  1:7352241/1‑93 (MQ=255)
                                                                                     cggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTcc    <  1:389597/93‑1 (MQ=255)
                                                                                     cggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTcc    >  2:9910364/1‑93 (MQ=255)
                                                                                      ggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCt   >  1:9444174/1‑93 (MQ=255)
                                                                                      ggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCt   <  1:6697548/93‑1 (MQ=255)
                                                                                       gcgcTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCtt  <  2:3562623/93‑1 (MQ=255)
                                                                                       gcgcTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCtt  >  1:7654771/1‑93 (MQ=255)
                                                                                       gcgcTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCtt  >  1:2715031/1‑93 (MQ=255)
                                                                                       gcgcTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCtt  >  1:1645751/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT  >  NC_000913/3918184‑3918363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 292 total aligned reads displayed.
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  NC_000913/3918181‑3918367
                                                                                             |                                                                                             
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGC                                                                                               >  SRR4302540.3013156/1‑94 (MQ=60)
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGC                                                                                               >  SRR4302540.4423545/1‑94 (MQ=60)
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGC                                                                                               >  SRR4302540.6002380/1‑94 (MQ=60)
  CGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTT                                                                                             >  SRR4302540.4712328/1‑94 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           <  SRR4302540.10075726/94‑1 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           <  SRR4302540.6909242/94‑1 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           <  SRR4302540.7622216/94‑1 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           >  SRR4302540.7723547/1‑94 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           >  SRR4302540.8360996/1‑94 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           <  SRR4302540.10075727/94‑1 (MQ=60)
     CTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTT                                                                                          >  SRR4302540.4536793/1‑94 (MQ=60)
     CTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTT                                                                                          >  SRR4302540.9267774/1‑94 (MQ=60)
     CTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTT                                                                                          >  SRR4302540.6401354/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGGGCTTGTTC                                                                                         >  SRR4302540.734351/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         <  SRR4302540.926617/94‑1 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         <  SRR4302540.4842402/94‑1 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         <  SRR4302540.496814/94‑1 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302540.6839445/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302540.7077240/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302540.7171324/1‑94 (MQ=60)
         AATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCC                                                                                      >  SRR4302540.10158572/1‑94 (MQ=60)
         AATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCC                                                                                      <  SRR4302540.2274037/94‑1 (MQ=60)
          ATTTCCGCTTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTGTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.3204054/94‑1 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     >  SRR4302540.788924/1‑94 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     >  SRR4302540.6238668/1‑94 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.4354032/94‑1 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.5593452/94‑1 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTGTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.3711204/94‑1 (MQ=60)
          ATTGCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.5593453/94‑1 (MQ=60)
          ATTCCCGCCTGCGCCGGGGCCACGATGTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302540.3711205/94‑1 (MQ=60)
             TCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCT                                                                                  <  SRR4302540.1091405/94‑1 (MQ=60)
             TCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCT                                                                                  >  SRR4302540.866539/1‑94 (MQ=60)
                GCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGA                                                                               <  SRR4302540.9670343/94‑1 (MQ=60)
                GCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGA                                                                               >  SRR4302540.5265656/1‑94 (MQ=60)
                 CCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGAG                                                                              >  SRR4302540.4372772/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302540.1673918/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302540.8809588/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302540.7586272/1‑94 (MQ=60)
                   TGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGA                                                                            <  SRR4302540.8005377/94‑1 (MQ=60)
                    GCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAT                                                                           <  SRR4302540.1612347/94‑1 (MQ=60)
                    GCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAT                                                                           <  SRR4302540.6764519/94‑1 (MQ=60)
                     CGCGTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          <  SRR4302540.3739378/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          >  SRR4302540.5823018/1‑94 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          <  SRR4302540.2874467/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          <  SRR4302540.820448/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          <  SRR4302540.820449/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          >  SRR4302540.4857585/1‑94 (MQ=60)
                      GCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTA                                                                         >  SRR4302540.1087945/1‑94 (MQ=60)
                      GCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTA                                                                         <  SRR4302540.3417446/94‑1 (MQ=60)
                       CCTGGGCCACGATTTTAGTTCGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAC                                                                        <  SRR4302540.2447825/94‑1 (MQ=60)
                       CCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAC                                                                        <  SRR4302540.3264359/94‑1 (MQ=60)
                       CCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAC                                                                        <  SRR4302540.1482475/94‑1 (MQ=60)
                        CTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTGGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACC                                                                       <  SRR4302540.5997349/94‑1 (MQ=60)
                        CTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACC                                                                       <  SRR4302540.2604750/94‑1 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      >  SRR4302540.7927679/1‑94 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      <  SRR4302540.6883971/94‑1 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      <  SRR4302540.9400057/94‑1 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      <  SRR4302540.9947297/94‑1 (MQ=60)
                          GGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTG                                                                     <  SRR4302540.3657513/94‑1 (MQ=60)
                          GGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTG                                                                     >  SRR4302540.9949066/1‑94 (MQ=60)
                          GGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTG                                                                     <  SRR4302540.2158401/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    >  SRR4302540.5352924/1‑94 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302540.2183143/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTGCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302540.7094990/94‑1 (MQ=60)
                            GCCACGATTTTAGTACGTTCCTATTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGG                                                                   <  SRR4302540.9031856/94‑1 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGC                                                                  >  SRR4302540.10110641/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTCCCTGGGCT                                                                 >  SRR4302540.3467381/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCT                                                                 >  SRR4302540.5943223/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCT                                                                 >  SRR4302540.7375587/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCT                                                                 >  SRR4302540.4975695/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCT                                                                 <  SRR4302540.1626020/94‑1 (MQ=60)
                                 GATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCC                                                              <  SRR4302540.7440264/94‑1 (MQ=60)
                                 GATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCC                                                              >  SRR4302540.6094327/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302540.799860/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302540.2125888/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             <  SRR4302540.1157701/94‑1 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             <  SRR4302540.4571089/94‑1 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302540.8029402/1‑94 (MQ=60)
                                   TTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGC                                                            >  SRR4302540.1660961/1‑94 (MQ=60)
                                   TTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGC                                                            >  SRR4302540.9945878/1‑94 (MQ=60)
                                     TTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCAGGGCTTCCGCTT                                                          >  SRR4302540.7789858/1‑94 (MQ=60)
                                      TAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTT                                                         >  SRR4302540.1041353/1‑94 (MQ=60)
                                      TAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTT                                                         >  SRR4302540.5894210/1‑94 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302540.5933447/1‑94 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302540.38194/1‑94 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302540.8015916/1‑94 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302540.8704030/1‑94 (MQ=60)
                                        GTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCG                                                       <  SRR4302540.1209320/94‑1 (MQ=60)
                                        GTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCG                                                       >  SRR4302540.1254752/1‑94 (MQ=60)
                                        GTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCG                                                       <  SRR4302540.3102256/94‑1 (MQ=60)
                                          ACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCT                                                     >  SRR4302540.9516220/1‑94 (MQ=60)
                                          ACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCT                                                     >  SRR4302540.1873319/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302540.3494187/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302540.1941203/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302540.8613063/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   >  SRR4302540.817270/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   >  SRR4302540.5366632/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   >  SRR4302540.4112436/1‑94 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  >  SRR4302540.1401562/1‑94 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  >  SRR4302540.8092813/1‑94 (MQ=60)
                                              TCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGCGGGGCGCGTGTTCGCCTGCTCGATGATTACCTAGGCTTCCGCTTTCGCTTTTT                                                 >  SRR4302540.7221328/1‑94 (MQ=60)
                                              TCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTT                                                 >  SRR4302540.1127559/1‑94 (MQ=60)
                                              TCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTT                                                 >  SRR4302540.8502406/1‑94 (MQ=60)
                                               CCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                >  SRR4302540.7315198/1‑94 (MQ=60)
                                               CCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                <  SRR4302540.671498/94‑1 (MQ=60)
                                               CCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                <  SRR4302540.1446160/94‑1 (MQ=60)
                                               CCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                <  SRR4302540.4726460/94‑1 (MQ=60)
                                               CCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                <  SRR4302540.1880737/94‑1 (MQ=60)
                                                CTGTTCTGCCTCAGCTTTCGCTTCGTTCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTT                                               >  SRR4302540.6509994/1‑94 (MQ=60)
                                                CTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTC                                               >  SRR4302540.1615017/1‑94 (MQ=60)
                                                CTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTC                                               >  SRR4302540.4595286/1‑94 (MQ=60)
                                                CTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTC                                               >  SRR4302540.6919622/1‑94 (MQ=60)
                                                CTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTC                                               >  SRR4302540.9142324/1‑94 (MQ=60)
                                                 TGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGGGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCA                                              >  SRR4302540.2924302/1‑94 (MQ=60)
                                                 TGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCA                                              <  SRR4302540.6620432/94‑1 (MQ=60)
                                                 TGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCA                                              <  SRR4302540.9765554/94‑1 (MQ=60)
                                                  GTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAG                                             <  SRR4302540.6716333/94‑1 (MQ=60)
                                                  GTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAG                                             >  SRR4302540.7757548/1‑94 (MQ=60)
                                                    TCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCT                                           >  SRR4302540.5942848/1‑94 (MQ=60)
                                                    TCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCT                                           <  SRR4302540.10012401/94‑1 (MQ=60)
                                                    TCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCT                                           >  SRR4302540.5435348/1‑94 (MQ=60)
                                                     TTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTG                                          <  SRR4302540.6291513/94‑1 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         >  SRR4302540.3232777/1‑94 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGCTGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         >  SRR4302540.6331048/1‑94 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTTAGCTGG                                         >  SRR4302540.708202/1‑94 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         >  SRR4302540.1828911/1‑94 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         >  SRR4302540.9637898/1‑94 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         <  SRR4302540.2814784/94‑1 (MQ=60)
                                                       GCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTTAGCTGGC                                        >  SRR4302540.6329944/1‑94 (MQ=60)
                                                       GCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGT                                        <  SRR4302540.7724594/94‑1 (MQ=60)
                                                        CCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTC                                       >  SRR4302540.4249242/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCGTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      <  SRR4302540.6400437/94‑1 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      <  SRR4302540.9343450/94‑1 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302540.4511852/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      <  SRR4302540.7765411/94‑1 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302540.3221143/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302540.2130709/1‑94 (MQ=60)
                                                          TCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGG                                     <  SRR4302540.3839758/94‑1 (MQ=60)
                                                          TCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGG                                     <  SRR4302540.4841506/94‑1 (MQ=60)
                                                           CAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGT                                    <  SRR4302540.5730189/94‑1 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   >  SRR4302540.7809216/1‑94 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   >  SRR4302540.10126962/1‑94 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   <  SRR4302540.8821650/94‑1 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   >  SRR4302540.762895/1‑94 (MQ=60)
                                                             GCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCG                                  <  SRR4302540.3590149/94‑1 (MQ=60)
                                                             GCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCG                                  >  SRR4302540.2283328/1‑94 (MQ=60)
                                                              CTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 <  SRR4302540.8995738/94‑1 (MQ=60)
                                                              CTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 >  SRR4302540.326825/1‑94 (MQ=60)
                                                              CTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 <  SRR4302540.8010313/94‑1 (MQ=60)
                                                               TTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCG                                >  SRR4302540.5825718/1‑94 (MQ=60)
                                                                TTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGC                               >  SRR4302540.2694999/1‑94 (MQ=60)
                                                                 TCGCTGCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCT                              <  SRR4302540.40953/94‑1 (MQ=60)
                                                                  CGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTG                             >  SRR4302540.5658450/1‑94 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           >  SRR4302540.1067749/1‑94 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           <  SRR4302540.2905049/94‑1 (MQ=60)
                                                                       CGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTT                        <  SRR4302540.4768026/94‑1 (MQ=60)
                                                                        GTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTT                       >  SRR4302540.7497159/1‑94 (MQ=60)
                                                                        CTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTT                       <  SRR4302540.4244735/94‑1 (MQ=60)
                                                                          CCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGTTGGCCTTTAC                     >  SRR4302540.8224741/1‑94 (MQ=60)
                                                                          CCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGC                     >  SRR4302540.6790089/1‑94 (MQ=60)
                                                                           CAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCA                    >  SRR4302540.8236820/1‑94 (MQ=60)
                                                                            AGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAG                   >  SRR4302540.7235814/1‑94 (MQ=60)
                                                                            AGAATCTGCGAGCGGCGCTTGATCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAA                   >  SRR4302540.5055984/1‑94 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  >  SRR4302540.8274512/1‑94 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  >  SRR4302540.8274514/1‑94 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  <  SRR4302540.5287999/94‑1 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  <  SRR4302540.1847705/94‑1 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  <  SRR4302540.9012570/94‑1 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  >  SRR4302540.8709257/1‑94 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 <  SRR4302540.2481869/94‑1 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 <  SRR4302540.3933166/94‑1 (MQ=60)
                                                                                TCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTC               <  SRR4302540.8375864/94‑1 (MQ=60)
                                                                                TCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTC               >  SRR4302540.8773171/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              >  SRR4302540.1862105/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              >  SRR4302540.9859917/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              <  SRR4302540.4637344/94‑1 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              >  SRR4302540.5470714/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              <  SRR4302540.3444326/94‑1 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              <  SRR4302540.1493674/94‑1 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              >  SRR4302540.1862102/1‑94 (MQ=60)
                                                                                   GCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAG            <  SRR4302540.5217419/94‑1 (MQ=60)
                                                                                    GGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGG           <  SRR4302540.1839853/94‑1 (MQ=60)
                                                                                    CGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGG           <  SRR4302540.8948446/94‑1 (MQ=60)
                                                                                    CGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGG           <  SRR4302540.5645447/94‑1 (MQ=60)
                                                                                     GAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGT          <  SRR4302540.2428666/94‑1 (MQ=60)
                                                                                     GAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGT          <  SRR4302540.8539054/94‑1 (MQ=60)
                                                                                      AGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTC         >  SRR4302540.4905870/1‑94 (MQ=60)
                                                                                       GCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCC        <  SRR4302540.183900/94‑1 (MQ=60)
                                                                                       GCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCC        >  SRR4302540.7434246/1‑94 (MQ=60)
                                                                                        CGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCT       >  SRR4302540.2075849/1‑94 (MQ=60)
                                                                                        CGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCT       <  SRR4302540.393623/94‑1 (MQ=60)
                                                                                        CGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCT       >  SRR4302540.9556296/1‑94 (MQ=60)
                                                                                        CGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCT       <  SRR4302540.4629660/94‑1 (MQ=60)
                                                                                         GGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT      <  SRR4302540.6771478/94‑1 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    <  SRR4302540.9379598/94‑1 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    >  SRR4302540.7963569/1‑94 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    <  SRR4302540.9930268/94‑1 (MQ=60)
                                                                                            GCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATG   >  SRR4302540.4161834/1‑94 (MQ=60)
                                                                                            GCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATG   >  SRR4302540.8997869/1‑94 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  <  SRR4302540.4174884/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  NC_000913/3918181‑3918367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑