Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,742	T→C	A320A (GCT→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,742	0	T	C	99.5%	1236.5 / ‑5.1	384	A320A (GCT→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (218/163); minor base A (2/0); total (221/163)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 407 total aligned reads displayed.

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

 aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  1:4151411/1‑93 (MQ=255)

 aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  2:9131918/1‑93 (MQ=255)

  tCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGccgctg                                                                                       >  2:3419385/1‑93 (MQ=255)

  tCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGccgctg                                                                                       >  2:7322967/1‑93 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  2:805110/93‑1 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  2:7607170/93‑1 (MQ=255)

       ggcggTATCGCCCAGTCTCGTTTGACTATGCTGCTGCTGCAGCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgc                                                                                  <  2:6653754/93‑1 (MQ=255)

        gcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgcg                                                                                 >  1:6591143/1‑93 (MQ=255)

         cggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCga                                                                                <  2:9525632/93‑1 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              <  2:6072189/93‑1 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             <  1:4550723/93‑1 (MQ=255)

              tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCg                                                                           >  1:2430508/1‑93 (MQ=255)

              tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCg                                                                           >  1:3826143/1‑93 (MQ=255)

                   cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCt                                                                      <  1:2884310/93‑1 (MQ=255)

                    aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  2:435656/1‑93 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:4735641/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  2:5825159/93‑1 (MQ=255)

                      tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtct                                                                   <  2:6312365/93‑1 (MQ=255)

                       ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  <  2:2226230/93‑1 (MQ=255)

                        tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  2:7514012/1‑93 (MQ=255)

                        tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  1:770122/1‑93 (MQ=255)

                            ttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgctg                                                             <  2:1191420/93‑1 (MQ=255)

                             tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            >  1:5104821/1‑93 (MQ=255)

                             tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            >  2:987273/1‑93 (MQ=255)

                              gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGta                                                           >  2:7488741/1‑93 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          <  2:9634221/93‑1 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  2:5672294/1‑93 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         >  2:2345943/1‑93 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         <  1:6109963/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        <  1:7515990/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        <  1:7872702/93‑1 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  1:9781220/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:4434103/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:8058617/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:5721193/1‑93 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:5169703/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  2:3555268/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  2:5244099/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  1:3211865/1‑93 (MQ=255)

                                      tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   <  1:9715682/93‑1 (MQ=255)

                                       gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAt                                                  <  1:424301/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 >  1:2054578/1‑93 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  1:1616287/1‑93 (MQ=255)

                                          gctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgc                                               >  2:1697507/1‑93 (MQ=255)

                                           ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              <  1:1496571/93‑1 (MQ=255)

                                           ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              <  2:8441646/93‑1 (MQ=255)

                                              cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccgca                                           <  1:8221872/93‑2 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCa                                          >  1:1101335/1‑93 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAAAAATTTATCGCCCCCa                                          >  1:7346725/1‑93 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         >  1:2480941/1‑93 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        >  1:2612884/1‑93 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        <  1:3692275/93‑1 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATAGCCGCCAGc                                        >  1:8912216/1‑93 (MQ=255)

                                                  tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                       >  2:2846069/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   <  1:6855859/93‑1 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:8688829/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:5003860/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:4538092/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:1188221/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                <  1:709503/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                <  1:6974591/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  2:3815052/1‑93 (MQ=255)

                                                          atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                               <  2:4663522/93‑1 (MQ=255)

                                                             ggCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                            <  1:9549866/93‑1 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  1:7459166/1‑93 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  2:10001066/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  1:2152512/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  2:4274433/1‑93 (MQ=255)

                                                                 aGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCt                        >  2:4139372/1‑93 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:9246666/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:7116352/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:9715389/93‑1 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  1:1936445/93‑1 (MQ=255)

                                                                   gTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTc                      <  1:7257895/93‑1 (MQ=255)

                                                                    ttCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                     >  2:822939/1‑93 (MQ=255)

                                                                    ttCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                     >  1:10026657/1‑93 (MQ=255)

                                                                     tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    >  1:8677565/1‑93 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:1838151/93‑1 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:3419385/93‑1 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   >  2:6946307/1‑93 (MQ=255)

                                                                       aGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAt                  >  1:268170/1‑93 (MQ=255)

                                                                        gtgtGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                 >  1:4393562/1‑93 (MQ=255)

                                                                         gggggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCGTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                <  1:9039919/90‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCCGGCGGCTTCGCATccc               <  2:8989611/93‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               <  2:6808669/93‑1 (MQ=255)

                                                                          gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               >  2:3683036/1‑93 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              >  1:5504828/1‑93 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              <  2:1457803/93‑1 (MQ=255)

                                                                            ggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCgg             >  2:5934866/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          >  1:5955726/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          <  1:173860/93‑1 (MQ=255)

                                                                                tATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATc         >  1:626400/1‑93 (MQ=255)

                                                                                 aTGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa        >  2:7790899/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       <  2:3194328/93‑1 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       >  1:1126672/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATACCGGTATCaa       >  1:8276680/1‑93 (MQ=255)

                                                                                    gCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATGCCGGTATAAAAg     >  2:2000204/1‑93 (MQ=255)

                                                                                     ccAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    >  1:9111268/1‑93 (MQ=255)

                                                                                      cAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg   >  2:6967537/1‑93 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  >  2:6494007/1‑93 (MQ=255)

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 229 total aligned reads displayed.

GACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  NC_000913/3925649‑3925835

GACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCC                                                                                               <  SRR4302540.4231916/94‑1 (MQ=60)

GACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCC                                                                                               <  SRR4302540.750143/94‑1 (MQ=60)

 ACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCG                                                                                              >  SRR4302540.4575075/1‑94 (MQ=60)

 ACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCG                                                                                              <  SRR4302540.3897940/94‑1 (MQ=60)

   CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCCGCTGCT                                                                                            >  SRR4302540.1167643/1‑94 (MQ=60)

   CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCT                                                                                            <  SRR4302540.3733352/94‑1 (MQ=60)

   CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCT                                                                                            >  SRR4302540.8887886/1‑94 (MQ=60)

   CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCT                                                                                            >  SRR4302540.4196371/1‑94 (MQ=60)

   CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGGGGGGAGTATGGCCAGCCGCT                                                                                            >  SRR4302540.344199/1‑94 (MQ=60)

    ATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTG                                                                                           >  SRR4302540.7089006/1‑94 (MQ=60)

       GGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTC                                                                                        <  SRR4302540.6143645/94‑1 (MQ=60)

       GGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTC                                                                                        <  SRR4302540.9028961/94‑1 (MQ=60)

          GGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGCGTATGGCCAGCCGCTGTTTGCG                                                                                     >  SRR4302540.7527968/1‑94 (MQ=60)

          GGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCG                                                                                     <  SRR4302540.7614475/94‑1 (MQ=60)

          GGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCG                                                                                     >  SRR4302540.6664142/1‑94 (MQ=60)

          GGCGGTATCGGCCAGTCCCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCG                                                                                     <  SRR4302540.4469789/94‑1 (MQ=60)

           GCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGA                                                                                    <  SRR4302540.2376117/94‑1 (MQ=60)

            gggtagcggccaggctcggttgaacattctgcctCTGCAACTGCCGCATATCGGCCAGGTTCATTGTGGAGTATGGCCAGCCGCTGTTCGCGAG                                                                                   <  SRR4302540.4747165/60‑1 (MQ=60)

            CGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAG                                                                                   <  SRR4302540.4747164/94‑1 (MQ=60)

            CGGTATCGGCCACTCTCGTTTGGCGATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTAGGGCCAGCCGCTGTTCGCGAG                                                                                   <  SRR4302540.276016/94‑1 (MQ=60)

             GGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCCGGCTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGcgg                                                                                  >  SRR4302540.3200321/1‑91 (MQ=60)

             GGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGA                                                                                  >  SRR4302540.4061715/1‑94 (MQ=60)

              GTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAG                                                                                 >  SRR4302540.7987115/1‑94 (MQ=60)

               TATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGC                                                                                >  SRR4302540.3708517/1‑94 (MQ=60)

               TATCGGCCAGTCTCGTTTGACTATGCTGCTCCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGC                                                                                >  SRR4302540.4911819/1‑94 (MQ=60)

                ATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCG                                                                               >  SRR4302540.2456697/1‑94 (MQ=60)

                ATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCG                                                                               >  SRR4302540.5165090/1‑94 (MQ=60)

                ATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCG                                                                               <  SRR4302540.1087858/94‑1 (MQ=60)

                ATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCG                                                                               >  SRR4302540.3867778/1‑94 (MQ=60)

                ATCGGCCAGTCTCGTTTGACTATGCTGCTGCCGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCG                                                                               >  SRR4302540.9257607/1‑94 (MQ=60)

                  CGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTC                                                                             >  SRR4302540.186848/1‑94 (MQ=60)

                   GGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCC                                                                            <  SRR4302540.1631636/94‑1 (MQ=60)

                   GGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCC                                                                            >  SRR4302540.7755078/1‑94 (MQ=60)

                    GCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCC                                                                           <  SRR4302540.8994557/94‑1 (MQ=60)

                     CCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCT                                                                          >  SRR4302540.3890602/1‑94 (MQ=60)

                     CCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCT                                                                          <  SRR4302540.7251104/94‑1 (MQ=60)

                     CCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCT                                                                          <  SRR4302540.9107548/94‑1 (MQ=60)

                     CCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCT                                                                          >  SRR4302540.7584060/1‑94 (MQ=60)

                      CAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTT                                                                         <  SRR4302540.4323721/94‑1 (MQ=60)

                      CAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTT                                                                         <  SRR4302540.2915703/94‑1 (MQ=60)

                      CAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTT                                                                         >  SRR4302540.5832416/1‑94 (MQ=60)

                       AGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTC                                                                        >  SRR4302540.8038467/1‑94 (MQ=60)

                        GTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCT                                                                       >  SRR4302540.8308701/1‑94 (MQ=60)

                        GTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCT                                                                       >  SRR4302540.3569546/1‑94 (MQ=60)

                        GTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCT                                                                       <  SRR4302540.4786199/94‑1 (MQ=60)

                        GTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCT                                                                       >  SRR4302540.1420232/1‑94 (MQ=60)

                        GTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCT                                                                       >  SRR4302540.9447736/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGCGCGTCCCTTCTCT                                                                     >  SRR4302540.7038588/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCT                                                                     >  SRR4302540.778317/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCT                                                                     >  SRR4302540.792169/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCT                                                                     >  SRR4302540.9675073/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCT                                                                     >  SRR4302540.4148310/1‑94 (MQ=60)

                          CTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCT                                                                     <  SRR4302540.7195246/94‑1 (MQ=60)

                            CGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGC                                                                   >  SRR4302540.7317122/1‑94 (MQ=60)

                             GTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCT                                                                  >  SRR4302540.739981/1‑94 (MQ=60)

                             GTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGCATGGCCAGCCGCTGGTAGCGAGAGTGCCCCTTCTCTGCT                                                                  >  SRR4302540.2646267/1‑94 (MQ=60)

                              TTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTG                                                                 <  SRR4302540.8040343/94‑1 (MQ=60)

                               TTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGT                                                                >  SRR4302540.5160084/1‑94 (MQ=60)

                               TTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGT                                                                >  SRR4302540.9871903/1‑94 (MQ=60)

                               TTAACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGGTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGGGAGCGTCCCTTCTCTTCTGT                                                                >  SRR4302540.9871902/1‑94 (MQ=60)

                                TGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTA                                                               >  SRR4302540.6710256/1‑94 (MQ=60)

                                 GACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAA                                                              >  SRR4302540.21369/1‑94 (MQ=60)

                                 GACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAA                                                              >  SRR4302540.3246281/1‑94 (MQ=60)

                                 GACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAA                                                              >  SRR4302540.6290958/1‑94 (MQ=60)

                                  ACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAAT                                                             <  SRR4302540.1972721/94‑1 (MQ=60)

                                   CTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATA                                                            >  SRR4302540.3390114/1‑94 (MQ=60)

                                   CTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATA                                                            <  SRR4302540.6178004/94‑1 (MQ=60)

                                   CTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATA                                                            >  SRR4302540.3154645/1‑94 (MQ=60)

                                   CTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATA                                                            >  SRR4302540.9895853/1‑94 (MQ=60)

                                   CTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATA                                                            >  SRR4302540.6114331/1‑94 (MQ=60)

                                    TATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           <  SRR4302540.7600023/94‑1 (MQ=60)

                                    TATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           <  SRR4302540.7964666/94‑1 (MQ=60)

                                    TATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           >  SRR4302540.2503743/1‑94 (MQ=60)

                                    TATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           <  SRR4302540.1765201/94‑1 (MQ=60)

                                    TATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           >  SRR4302540.6775789/1‑94 (MQ=60)

                                    TATGCTGCGGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAA                                                           <  SRR4302540.734124/94‑1 (MQ=60)

                                     ATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTcgactaat                                                          >  SRR4302540.5784413/1‑86 (MQ=60)

                                     ATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAT                                                          >  SRR4302540.4482146/1‑94 (MQ=60)

                                     ATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAT                                                          >  SRR4302540.8152178/1‑94 (MQ=60)

                                     ATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAT                                                          >  SRR4302540.5225947/1‑94 (MQ=60)

                                      TGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATT                                                         >  SRR4302540.3246730/1‑94 (MQ=60)

                                      TGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATT                                                         <  SRR4302540.1657571/94‑1 (MQ=60)

                                       GCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTT                                                        <  SRR4302540.1889843/94‑1 (MQ=60)

                                       GCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTT                                                        <  SRR4302540.832630/94‑1 (MQ=60)

                                       GCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTT                                                        >  SRR4302540.7475596/1‑94 (MQ=60)

                                        CTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTA                                                       >  SRR4302540.4381173/1‑94 (MQ=60)

                                        CTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTA                                                       >  SRR4302540.6825303/1‑94 (MQ=60)

                                         TGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGTTGTAATAATTTAT                                                      >  SRR4302540.5558993/1‑94 (MQ=60)

                                         TGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAT                                                      <  SRR4302540.1138289/94‑1 (MQ=60)

                                          GGTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGGTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     <  SRR4302540.1554989/94‑1 (MQ=60)

                                          GCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     >  SRR4302540.2513983/1‑94 (MQ=60)

                                          GCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     <  SRR4302540.4525554/94‑1 (MQ=60)

                                          GCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     >  SRR4302540.2076694/1‑94 (MQ=60)

                                          GCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     <  SRR4302540.3810456/94‑1 (MQ=60)

                                          GCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATC                                                     <  SRR4302540.428771/94‑1 (MQ=60)

                                           CTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCG                                                    >  SRR4302540.1634017/1‑94 (MQ=60)

                                           CTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCG                                                    <  SRR4302540.7150166/94‑1 (MQ=60)

                                            TGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGC                                                   <  SRR4302540.1267590/94‑1 (MQ=60)

                                              CTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCG                                                 <  SRR4302540.1513049/94‑1 (MQ=60)

                                              CTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCG                                                 <  SRR4302540.3549993/94‑1 (MQ=60)

                                               TGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGC                                                >  SRR4302540.4684170/1‑94 (MQ=60)

                                               TGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGC                                                >  SRR4302540.9164760/1‑94 (MQ=60)

                                               TGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGC                                                >  SRR4302540.5983281/1‑94 (MQ=60)

                                                GCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCC                                               >  SRR4302540.7717288/1‑94 (MQ=60)

                                                 CAACTGCCGCTTATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCA                                              <  SRR4302540.2011154/94‑1 (MQ=60)

                                                 CAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCA                                              <  SRR4302540.8317445/94‑1 (MQ=60)

                                                 CAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCA                                              <  SRR4302540.2602648/94‑1 (MQ=60)

                                                 CAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCA                                              >  SRR4302540.1112681/1‑94 (MQ=60)

                                                 CAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCAA                                              <  SRR4302540.8317444/94‑1 (MQ=60)

                                                  AACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAG                                             >  SRR4302540.2507669/1‑94 (MQ=60)

                                                  AACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAG                                             <  SRR4302540.4267601/94‑1 (MQ=60)

                                                   ACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGC                                            >  SRR4302540.1978076/1‑94 (MQ=60)

                                                   ACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGC                                            >  SRR4302540.2641094/1‑94 (MQ=60)

                                                    CTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCG                                           >  SRR4302540.8343612/1‑94 (MQ=60)

                                                    CTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCG                                           <  SRR4302540.6505766/94‑1 (MQ=60)

                                                    CTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCG                                           <  SRR4302540.3732601/94‑1 (MQ=60)

                                                    CTGCCGAATATCGGCCGGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCG                                           <  SRR4302540.2132398/94‑1 (MQ=60)

                                                     TGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGT                                          >  SRR4302540.9962277/1‑94 (MQ=60)

                                                     TGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGT                                          >  SRR4302540.9962276/1‑94 (MQ=60)

                                                     TGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGT                                          >  SRR4302540.5630529/1‑94 (MQ=60)

                                                      GCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTC                                         >  SRR4302540.5204331/1‑94 (MQ=60)

                                                       CCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCT                                        >  SRR4302540.4547996/1‑94 (MQ=60)

                                                        CGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTG                                       <  SRR4302540.8733502/94‑1 (MQ=60)

                                                        CGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTG                                       >  SRR4302540.1784019/1‑94 (MQ=60)

                                                         GCATATCGGCTAGGTTCAGTGTGGAGTATGGCCAGCCGTTGTTCGCGAGAGCGGCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGA                                      <  SRR4302540.5135067/94‑1 (MQ=60)

                                                         GCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGA                                      <  SRR4302540.6931814/94‑1 (MQ=60)

                                                           ATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGC                                    >  SRR4302540.5234184/1‑94 (MQ=60)

                                                           ATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGC                                    >  SRR4302540.1200469/1‑94 (MQ=60)

                                                            TATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCA                                   <  SRR4302540.2460750/94‑1 (MQ=60)

                                                            TATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCA                                   <  SRR4302540.7052098/94‑1 (MQ=60)

                                                             ATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAG                                  <  SRR4302540.6179250/94‑1 (MQ=60)

                                                             ATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAG                                  <  SRR4302540.5162155/94‑1 (MQ=60)

                                                             ATCGGCAAGGTTCAGTGTGGAGGATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAG                                  <  SRR4302540.6179249/94‑1 (MQ=60)

                                                              TCGGCTAGATTCAGTGTGGAGCATGGCCAGCCGCTGTCCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGG                                 <  SRR4302540.1142102/94‑1 (MQ=60)

                                                              TCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGG                                 >  SRR4302540.5704009/1‑94 (MQ=60)

                                                               CGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGC                                <  SRR4302540.2870388/94‑1 (MQ=60)

                                                               CGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGC                                >  SRR4302540.5963180/1‑94 (MQ=60)

                                                               CGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGC                                <  SRR4302540.4420852/94‑1 (MQ=60)

                                                                GGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCGGGCG                               >  SRR4302540.7542335/1‑94 (MQ=60)

                                                                GGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCG                               <  SRR4302540.9662830/94‑1 (MQ=60)

                                                                GGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCG                               <  SRR4302540.4270936/94‑1 (MQ=60)

                                                                GGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCG                               <  SRR4302540.1661055/94‑1 (MQ=60)

                                                                GGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCG                               >  SRR4302540.7542336/1‑94 (MQ=60)

                                                                 GCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGG                              >  SRR4302540.8882236/1‑94 (MQ=60)

                                                                 GCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGG                              >  SRR4302540.2291737/1‑94 (MQ=60)

                                                                  CCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGC                             >  SRR4302540.2175665/1‑94 (MQ=60)

                                                                  CCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGC                             >  SRR4302540.10157946/1‑94 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          <  SRR4302540.1957543/94‑1 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          >  SRR4302540.5633249/1‑94 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          >  SRR4302540.4472083/1‑94 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          >  SRR4302540.1571900/1‑94 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          >  SRR4302540.1115193/1‑94 (MQ=60)

                                                                     GGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTC                          <  SRR4302540.701910/94‑1 (MQ=60)

                                                                      GTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCG                         <  SRR4302540.7338878/94‑1 (MQ=60)

                                                                      GTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCG                         <  SRR4302540.3301593/94‑1 (MQ=60)

                                                                      GTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCG                         >  SRR4302540.10143321/1‑94 (MQ=60)

                                                                      GTTCAGTGTGGAGTATGCCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCG                         <  SRR4302540.1037983/94‑1 (MQ=60)

                                                                       TTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGC                        >  SRR4302540.8778344/1‑94 (MQ=60)

                                                                        TCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCA                       >  SRR4302540.1766802/1‑94 (MQ=60)

                                                                        TCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCA                       <  SRR4302540.7067619/94‑1 (MQ=60)

                                                                         CAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAT                      <  SRR4302540.1858282/94‑1 (MQ=60)

                                                                         CAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAT                      >  SRR4302540.270812/1‑94 (MQ=60)

                                                                         CAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAT                      <  SRR4302540.7543867/94‑1 (MQ=60)

                                                                          AGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATC                     >  SRR4302540.4441238/1‑94 (MQ=60)

                                                                           GTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCC                    >  SRR4302540.8586855/1‑94 (MQ=60)

                                                                           GTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCC                    >  SRR4302540.8586856/1‑94 (MQ=60)

                                                                            gggGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCGTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCC                   <  SRR4302540.9148429/91‑1 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  <  SRR4302540.9860795/94‑1 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  <  SRR4302540.977090/94‑1 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  <  SRR4302540.2613314/94‑1 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  >  SRR4302540.5565454/1‑94 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  <  SRR4302540.8300321/94‑1 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  >  SRR4302540.2199071/1‑94 (MQ=60)

                                                                             GTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCG                  >  SRR4302540.4500829/1‑94 (MQ=60)

                                                                               GGGGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGT                <  SRR4302540.7866644/94‑1 (MQ=60)

                                                                                 AGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAT              >  SRR4302540.7403872/1‑94 (MQ=60)

                                                                                 AGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAT              >  SRR4302540.6021594/1‑94 (MQ=60)

                                                                                  GTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATC             <  SRR4302540.175597/94‑1 (MQ=60)

                                                                                  GTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATC             >  SRR4302540.633067/1‑94 (MQ=60)

                                                                                  GTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATC             <  SRR4302540.6514319/94‑1 (MQ=60)

                                                                                  GTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATC             >  SRR4302540.2724333/1‑94 (MQ=60)

                                                                                    ATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAA           >  SRR4302540.2799399/1‑94 (MQ=60)

                                                                                    ATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAA           >  SRR4302540.1138394/1‑94 (MQ=60)

                                                                                    ATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAA           >  SRR4302540.9769100/1‑94 (MQ=60)

                                                                                     TGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAA          >  SRR4302540.1144930/1‑94 (MQ=60)

                                                                                       GCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGC        <  SRR4302540.9130260/94‑1 (MQ=60)

                                                                                       GCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGC        <  SRR4302540.9158265/94‑1 (MQ=60)

                                                                                       GCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGC        >  SRR4302540.9220771/1‑94 (MQ=60)

                                                                                       GCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCGGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGC        <  SRR4302540.9174695/94‑1 (MQ=60)

                                                                                         CAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCTCCGCCAGCGTCTGAGCCGGCGGCTTCGCATCCCGGTATCAAAGCGC      >  SRR4302540.4898212/1‑94 (MQ=60)

                                                                                         CAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC      >  SRR4302540.2705969/1‑94 (MQ=60)

                                                                                          AGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCC     >  SRR4302540.5317216/1‑94 (MQ=60)

                                                                                          AGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCC     <  SRR4302540.8126854/94‑1 (MQ=60)

                                                                                          AGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCC     <  SRR4302540.5625538/94‑1 (MQ=60)

                                                                                            CCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAG   >  SRR4302540.8106661/1‑94 (MQ=60)

                                                                                             CGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  SRR4302540.2533748/1‑94 (MQ=60)

                                                                                             CGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  SRR4302540.6017814/1‑94 (MQ=60)

                                                                                             CGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  SRR4302540.5853920/1‑94 (MQ=60)

                                                                                             CGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  SRR4302540.4852527/1‑94 (MQ=60)

                                                                                             CGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  SRR4302540.8232186/1‑94 (MQ=60)

GACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGA  >  NC_000913/3925649‑3925835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`