Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,224 A→G I40V (ATC→GTC)  ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,2240AG99.8% 1364.4 / ‑5.6 426I40V (ATC→GTC) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (187/238);  total (187/239)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 438 total aligned reads displayed.

TGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGC  >  NC_000913/3950134‑3950313
                                                                                          |                                                                                         
tGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTc                                                                                         <  1:1161076/93‑1 (MQ=255)
tGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTc                                                                                         >  1:7761146/1‑93 (MQ=255)
   ttCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGt                                                                                      >  2:4851167/1‑93 (MQ=255)
   ttCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGt                                                                                      <  2:8085094/93‑1 (MQ=255)
   ttCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCAGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGt                                                                                      <  2:6370847/93‑1 (MQ=255)
    tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGtt                                                                                     <  2:2822784/93‑1 (MQ=255)
    tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGtt                                                                                     <  1:7929640/93‑1 (MQ=255)
     cAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTg                                                                                    <  1:1271292/93‑1 (MQ=255)
      aaTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGc                                                                                   <  2:9509678/93‑1 (MQ=255)
      aaTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGc                                                                                   <  1:4523340/93‑1 (MQ=255)
      aaTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGc                                                                                   >  2:8228719/1‑93 (MQ=255)
        tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTa                                                                                 <  2:7559717/93‑1 (MQ=255)
          gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACg                                                                               <  2:6633567/93‑1 (MQ=255)
           ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGa                                                                              <  1:6478183/93‑1 (MQ=255)
              gaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTc                                                                           >  2:1671183/1‑93 (MQ=255)
                tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGc                                                                         >  2:6307945/1‑93 (MQ=255)
                tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGc                                                                         >  1:1701426/1‑93 (MQ=255)
                 ggTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGca                                                                        >  1:2254065/1‑93 (MQ=255)
                 ggTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGca                                                                        <  2:4449159/93‑1 (MQ=255)
                    tCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACaa                                                                     >  2:2120362/1‑93 (MQ=255)
                     cGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACaaa                                                                    <  2:5319917/93‑1 (MQ=255)
                      gCTGGGAAGACGCGGAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAg                                                                   <  1:4595684/93‑1 (MQ=255)
                      gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAg                                                                   <  1:4073258/93‑1 (MQ=255)
                      gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAg                                                                   <  2:9679930/93‑1 (MQ=255)
                       cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAgg                                                                  >  1:6751604/1‑93 (MQ=255)
                        tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGa                                                                 >  2:3257699/1‑93 (MQ=255)
                         gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACACAGGAc                                                                >  1:1904512/1‑93 (MQ=255)
                         gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAc                                                                >  1:9318373/1‑93 (MQ=255)
                         gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAc                                                                <  2:6025515/93‑1 (MQ=255)
                          ggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAcc                                                               >  2:3833926/1‑93 (MQ=255)
                           gAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCg                                                              <  1:7622701/93‑1 (MQ=255)
                            aaGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCgg                                                             <  1:9662700/93‑1 (MQ=255)
                             aGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGt                                                            >  1:4647063/1‑93 (MQ=255)
                              gACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGtt                                                           <  2:2499230/93‑1 (MQ=255)
                               aCGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTg                                                          <  2:1551070/93‑1 (MQ=255)
                                 gcgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTa                                                        <  1:7018467/93‑1 (MQ=255)
                                  cgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                                       <  1:6232788/93‑1 (MQ=255)
                                  cgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                                       >  2:7232222/1‑93 (MQ=255)
                                   gAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                                                      <  1:5727318/93‑1 (MQ=255)
                                   gAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                                                      >  2:7956527/1‑93 (MQ=255)
                                   gAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                                                      <  2:1306351/93‑1 (MQ=255)
                                   cagggTGCATGTGATGTCGCACTGTCTCCGCTCTGGCTCTTGGATTTTTGAAGTCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                                                      <  2:8894918/90‑1 (MQ=255)
                                      ggTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCg                                                   >  2:9935706/1‑93 (MQ=255)
                                      ggTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTACg                                                   >  2:5572616/1‑93 (MQ=255)
                                       gTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                                                  >  2:4549288/1‑93 (MQ=255)
                                       gTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                                                  >  2:1034329/1‑93 (MQ=255)
                                         gCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                                                >  2:571262/1‑93 (MQ=255)
                                          cATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCAt                                               <  1:9195655/93‑1 (MQ=255)
                                          cATGTGATGTCGCACGCCCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCAt                                               <  1:3172758/93‑1 (MQ=255)
                                           aTGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATc                                              <  2:8353841/93‑1 (MQ=255)
                                            tgtgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCg                                             >  1:8673826/1‑93 (MQ=255)
                                            tgtgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCg                                             >  2:116898/1‑93 (MQ=255)
                                               gATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGa                                          >  2:4984493/1‑93 (MQ=255)
                                                aTGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAg                                         >  1:3461565/1‑93 (MQ=255)
                                                 tGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGGTGTATTCCGCCATCGTGAGc                                        >  2:3998416/1‑93 (MQ=255)
                                                   tCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat                                      >  2:5824258/1‑93 (MQ=255)
                                                   tCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGCCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat                                      <  2:6889234/93‑1 (MQ=255)
                                                    cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata                                     <  1:6902299/93‑1 (MQ=255)
                                                     gCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat                                    <  1:376861/93‑1 (MQ=255)
                                                     gCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat                                    <  2:5512310/93‑1 (MQ=255)
                                                     gCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat                                    <  2:3561349/93‑1 (MQ=255)
                                                      cACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATg                                   >  2:3019545/1‑93 (MQ=255)
                                                       aCGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc                                  <  1:5209607/93‑1 (MQ=255)
                                                        cgcgATCCACTATGGCCCTTCGGATTTTGAAGGCGTCGGTTGCTACGACTCGCACAAAGGACCGTTTGTATTCCGTTATAGTGAGCATATGCa                                 <  2:6928452/93‑1 (MQ=255)
                                                         gcgcTGCTCTATGGCACTTCGGTTTGTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGTTTGTATTCCGCCATCGTGAGCATATGCAg                                <  2:9030003/93‑1 (MQ=255)
                                                          cgcTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGc                               <  1:5381538/93‑1 (MQ=255)
                                                          cgcTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGc                               >  1:9887152/1‑93 (MQ=255)
                                                            cTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt                             <  1:7122640/93‑1 (MQ=255)
                                                            cTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt                             <  2:8782289/93‑1 (MQ=255)
                                                            cTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt                             <  1:7370161/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTTGGTTTTTGAAGGCGGCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  2:1949142/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  2:415829/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  1:299291/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            >  1:9065986/1‑93 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  1:2776948/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  2:2740060/93‑1 (MQ=255)
                                                             tGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc                            <  2:9851469/93‑1 (MQ=255)
                                                                 cTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa                        <  2:9376612/93‑1 (MQ=255)
                                                                 cTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa                        <  2:8652471/93‑1 (MQ=255)
                                                                 cTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa                        <  1:6079165/93‑1 (MQ=255)
                                                                  tATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAt                       >  1:5011289/1‑93 (MQ=255)
                                                                  tATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAt                       >  1:6371513/1‑93 (MQ=255)
                                                                   aTGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATg                      >  2:2407641/1‑93 (MQ=255)
                                                                    tGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGa                     >  2:9104617/1‑93 (MQ=255)
                                                                    tGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGa                     <  1:9000235/93‑1 (MQ=255)
                                                                        tcTTCGGTTTGTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTcc                 <  1:1517967/92‑1 (MQ=255)
                                                                        ccTTCGGTTTTTGAAGGCGTCCGTTGCTACGACGCGCACAAATGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTcc                 <  2:7083569/92‑1 (MQ=255)
                                                                           tCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGcc              <  2:1493184/93‑1 (MQ=255)
                                                                           tCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGcc              >  1:7297285/1‑93 (MQ=255)
                                                                             ggTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCaa            >  1:1024694/1‑93 (MQ=255)
                                                                               tttttGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCaaaa          <  2:7371838/93‑1 (MQ=255)
                                                                               tttttGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCaaaa          >  2:1850922/1‑93 (MQ=255)
                                                                               gttttGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCaaaa          <  1:2350203/92‑1 (MQ=255)
                                                                                ttttGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAt         >  1:9737287/1‑93 (MQ=255)
                                                                                   tGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTa      >  1:3365202/1‑93 (MQ=255)
                                                                                   tGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTa      >  1:8873887/1‑93 (MQ=255)
                                                                                     aaGGCGTCCGTTTCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATc    <  2:5227527/93‑1 (MQ=255)
                                                                                     aaGGCGTCCGTTGCGACGACTCGCACAAAGGACCGGTTGGATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATc    <  1:2123082/93‑1 (MQ=255)
                                                                                       ggCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGc  >  1:4254834/1‑93 (MQ=255)
                                                                                       ggCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGc  >  1:9495158/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGC  >  NC_000913/3950134‑3950313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 217 total aligned reads displayed.
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTCC  >  NC_000913/3950131‑3950317
                                                                                             |                                                                                             
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCG                                                                                               <  SRR4302540.7671193/94‑1 (MQ=60)
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCG                                                                                               <  SRR4302540.2067197/94‑1 (MQ=60)
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCG                                                                                               <  SRR4302540.5698665/94‑1 (MQ=60)
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCG                                                                                               >  SRR4302540.8687099/1‑94 (MQ=60)
 TTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGT                                                                                              <  SRR4302540.7627024/94‑1 (MQ=60)
 TTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGT                                                                                              <  SRR4302540.977974/94‑1 (MQ=60)
 TTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGT                                                                                              >  SRR4302540.2792738/1‑94 (MQ=60)
  TTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTC                                                                                             >  SRR4302540.7851843/1‑94 (MQ=60)
  TTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTC                                                                                             >  SRR4302540.7851842/1‑94 (MQ=60)
   TGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCC                                                                                            <  SRR4302540.1173140/94‑1 (MQ=60)
   TGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCC                                                                                            <  SRR4302540.3374833/94‑1 (MQ=60)
   TGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCC                                                                                            <  SRR4302540.6611861/94‑1 (MQ=60)
     GTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGT                                                                                          >  SRR4302540.6239199/1‑94 (MQ=60)
     GTGCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGT                                                                                          <  SRR4302540.2329292/94‑1 (MQ=60)
      TTCAATGTGTAGATGTTTCTCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTT                                                                                         <  SRR4302540.5353612/94‑1 (MQ=60)
      TTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTT                                                                                         >  SRR4302540.5877698/1‑94 (MQ=60)
       TCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTG                                                                                        <  SRR4302540.8022060/94‑1 (MQ=60)
       TCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTG                                                                                        <  SRR4302540.7050883/94‑1 (MQ=60)
        CAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGC                                                                                       >  SRR4302540.2687565/1‑94 (MQ=60)
        CAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGC                                                                                       <  SRR4302540.1284375/94‑1 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      >  SRR4302540.7901557/1‑94 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      >  SRR4302540.7444817/1‑94 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      >  SRR4302540.9800956/1‑94 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      >  SRR4302540.8936741/1‑94 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      <  SRR4302540.4572326/94‑1 (MQ=60)
         AATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCT                                                                                      <  SRR4302540.2526695/94‑1 (MQ=60)
          ATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTA                                                                                     >  SRR4302540.8443353/1‑94 (MQ=60)
          ATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTA                                                                                     >  SRR4302540.3918290/1‑94 (MQ=60)
           TGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTAC                                                                                    >  SRR4302540.9508129/1‑94 (MQ=60)
            GGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACG                                                                                   <  SRR4302540.541540/94‑1 (MQ=60)
              GGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGAC                                                                                 <  SRR4302540.6550253/94‑1 (MQ=60)
               GAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACT                                                                                >  SRR4302540.1234364/1‑94 (MQ=60)
                AGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTC                                                                               <  SRR4302540.3297731/94‑1 (MQ=60)
                AGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTC                                                                               <  SRR4302540.1651853/94‑1 (MQ=60)
                 GATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCG                                                                              >  SRR4302540.6888947/1‑94 (MQ=60)
                  ATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGC                                                                             >  SRR4302540.1719846/1‑94 (MQ=60)
                  ATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGC                                                                             >  SRR4302540.6569499/1‑94 (MQ=60)
                   TGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCA                                                                            >  SRR4302540.2278256/1‑94 (MQ=60)
                    GGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCAC                                                                           >  SRR4302540.4689400/1‑94 (MQ=60)
                    GGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCAC                                                                           >  SRR4302540.4689399/1‑94 (MQ=60)
                        CGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGTTACGACTCGCACAAAG                                                                       >  SRR4302540.8040075/1‑94 (MQ=60)
                        CGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAG                                                                       >  SRR4302540.927451/1‑94 (MQ=60)
                        CGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAG                                                                       <  SRR4302540.8978541/94‑1 (MQ=60)
                         GCTGGGAAGACGCGGAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGG                                                                      <  SRR4302540.4645114/94‑1 (MQ=60)
                         GCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGG                                                                      <  SRR4302540.4117163/94‑1 (MQ=60)
                         GCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGG                                                                      >  SRR4302540.6826204/1‑94 (MQ=60)
                         GCTAGCAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGG                                                                      <  SRR4302540.4117162/94‑1 (MQ=60)
                          CTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGA                                                                     <  SRR4302540.5238903/94‑1 (MQ=60)
                           TGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAC                                                                    >  SRR4302540.9429568/1‑94 (MQ=60)
                           TGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAC                                                                    <  SRR4302540.3744149/94‑1 (MQ=60)
                           TGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGAC                                                                    >  SRR4302540.55266/1‑94 (MQ=60)
                            GGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACC                                                                   <  SRR4302540.515250/94‑1 (MQ=60)
                            GGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACC                                                                   <  SRR4302540.9137466/94‑1 (MQ=60)
                            GGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACC                                                                   <  SRR4302540.9835489/94‑1 (MQ=60)
                             GGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCG                                                                  <  SRR4302540.734417/94‑1 (MQ=60)
                              GAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGG                                                                 <  SRR4302540.7708427/94‑1 (MQ=60)
                              GAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGG                                                                 <  SRR4302540.6739052/94‑1 (MQ=60)
                               AAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGT                                                                >  SRR4302540.4696806/1‑94 (MQ=60)
                               AAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGT                                                                <  SRR4302540.9776438/94‑1 (MQ=60)
                               AAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGT                                                                <  SRR4302540.7575737/94‑1 (MQ=60)
                                 GACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTG                                                              <  SRR4302540.6453290/94‑1 (MQ=60)
                                 GACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTG                                                              <  SRR4302540.6937072/94‑1 (MQ=60)
                                  ACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGT                                                             <  SRR4302540.1110963/94‑1 (MQ=60)
                                  ACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGT                                                             <  SRR4302540.1110964/94‑1 (MQ=60)
                                   CGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTA                                                            <  SRR4302540.1827254/94‑1 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           <  SRR4302540.7743666/94‑1 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           <  SRR4302540.4644961/94‑1 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           <  SRR4302540.9945968/94‑1 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           >  SRR4302540.7759369/1‑94 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           <  SRR4302540.7096416/94‑1 (MQ=60)
                                    GCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAT                                                           <  SRR4302540.2336364/94‑1 (MQ=60)
                                     CGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATT                                                          <  SRR4302540.6302107/94‑1 (MQ=60)
                                     CGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATT                                                          >  SRR4302540.1745947/1‑94 (MQ=60)
                                     CGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATT                                                          >  SRR4302540.909159/1‑94 (MQ=60)
                                     CGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATT                                                          <  SRR4302540.237252/94‑1 (MQ=60)
                                      GAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTCTTC                                                         >  SRR4302540.7570895/1‑94 (MQ=60)
                                      GAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTC                                                         >  SRR4302540.6316647/1‑94 (MQ=60)
                                      GAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTC                                                         <  SRR4302540.5790390/94‑1 (MQ=60)
                                      GAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTC                                                         >  SRR4302540.3272814/1‑94 (MQ=60)
                                       AAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCC                                                        >  SRR4302540.3474189/1‑94 (MQ=60)
                                        AGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCG                                                       <  SRR4302540.2149001/94‑1 (MQ=60)
                                         GGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGC                                                      >  SRR4302540.1059297/1‑94 (MQ=60)
                                         GGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGC                                                      >  SRR4302540.1511953/1‑94 (MQ=60)
                                          GTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCC                                                     >  SRR4302540.9140301/1‑94 (MQ=60)
                                          GTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCC                                                     >  SRR4302540.5550227/1‑94 (MQ=60)
                                          GTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCC                                                     <  SRR4302540.2242923/94‑1 (MQ=60)
                                           TGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCA                                                    >  SRR4302540.2040559/1‑94 (MQ=60)
                                            GCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCAT                                                   >  SRR4302540.9905330/1‑94 (MQ=60)
                                            GCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCAT                                                   <  SRR4302540.3079152/94‑1 (MQ=60)
                                             CATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATC                                                  <  SRR4302540.7920574/94‑1 (MQ=60)
                                             CATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATC                                                  <  SRR4302540.9305960/94‑1 (MQ=60)
                                             CATGTGATGTCGCACGCCCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATC                                                  <  SRR4302540.3207249/94‑1 (MQ=60)
                                             CATGTGATGCCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATC                                                  <  SRR4302540.9469726/94‑1 (MQ=60)
                                              ATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCG                                                 >  SRR4302540.8774574/1‑94 (MQ=60)
                                              ATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCG                                                 >  SRR4302540.3403305/1‑94 (MQ=60)
                                              ATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCG                                                 >  SRR4302540.2982291/1‑94 (MQ=60)
                                              ATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCG                                                 >  SRR4302540.7167499/1‑94 (MQ=60)
                                               TGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGT                                                <  SRR4302540.6549999/94‑1 (MQ=60)
                                                GTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCATTGCTCCGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTG                                               <  SRR4302540.3899005/94‑1 (MQ=60)
                                                 TGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGA                                              >  SRR4302540.1320605/1‑94 (MQ=60)
                                                 GGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTTCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGA                                              <  SRR4302540.3542465/94‑1 (MQ=60)
                                                  GATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAG                                             >  SRR4302540.3499242/1‑94 (MQ=60)
                                                   ATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGC                                            <  SRR4302540.7176887/94‑1 (MQ=60)
                                                   ATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGC                                            >  SRR4302540.6250502/1‑94 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.9360898/94‑1 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.7331458/94‑1 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.6979023/94‑1 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.7832902/94‑1 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.4728428/94‑1 (MQ=60)
                                                       CGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATAT                                        <  SRR4302540.9392709/94‑1 (MQ=60)
                                                        GCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATG                                       >  SRR4302540.3021508/1‑94 (MQ=60)
                                                        GCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATG                                       <  SRR4302540.380760/94‑1 (MQ=60)
                                                        GCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATG                                       >  SRR4302540.6911836/1‑94 (MQ=60)
                                                         CACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGC                                      <  SRR4302540.9450457/94‑1 (MQ=60)
                                                         CACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGC                                      <  SRR4302540.6136727/94‑1 (MQ=60)
                                                          ACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCA                                     <  SRR4302540.5266363/94‑1 (MQ=60)
                                                           CGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAG                                    >  SRR4302540.6429115/1‑94 (MQ=60)
                                                            GCGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGC                                   >  SRR4302540.10002600/1‑94 (MQ=60)
                                                             CGCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCG                                  <  SRR4302540.5440728/94‑1 (MQ=60)
                                                              GCTGCACTATGGCACTTCGGTTTTTGTAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT                                 >  SRR4302540.6965118/1‑94 (MQ=60)
                                                              GCTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT                                 <  SRR4302540.5298012/94‑1 (MQ=60)
                                                               CTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTTCGCCATCGTGAGCATATGCAGCGTC                                >  SRR4302540.1220861/1‑94 (MQ=60)
                                                               CTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTC                                <  SRR4302540.7201952/94‑1 (MQ=60)
                                                               CTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTC                                >  SRR4302540.9174843/1‑94 (MQ=60)
                                                               CTGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTC                                <  SRR4302540.7452316/94‑1 (MQ=60)
                                                                TGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCT                               <  SRR4302540.302249/94‑1 (MQ=60)
                                                                TGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCT                               <  SRR4302540.2806959/94‑1 (MQ=60)
                                                                TGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCT                               <  SRR4302540.6138155/94‑1 (MQ=60)
                                                                TGCACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCT                               <  SRR4302540.7314611/94‑1 (MQ=60)
                                                                  CACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGC                             >  SRR4302540.9464001/1‑94 (MQ=60)
                                                                   ACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA                            <  SRR4302540.4526586/94‑1 (MQ=60)
                                                                   ACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA                            >  SRR4302540.1480306/1‑94 (MQ=60)
                                                                   ACTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA                            >  SRR4302540.9166927/1‑94 (MQ=60)
                                                                    agcgcttACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.8161191/87‑1 (MQ=60)
                                                                    agcgcttACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.6428749/87‑1 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           >  SRR4302540.180795/1‑94 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           >  SRR4302540.5065549/1‑94 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.8973074/94‑1 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.7425377/94‑1 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.4378536/94‑1 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.6146763/94‑1 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           >  SRR4302540.7279043/1‑94 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           >  SRR4302540.6442458/1‑94 (MQ=60)
                                                                    CTATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCAT                           <  SRR4302540.9365775/94‑1 (MQ=60)
                                                                     TATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATG                          <  SRR4302540.6507900/94‑1 (MQ=60)
                                                                      ATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGA                         >  SRR4302540.3550513/1‑94 (MQ=60)
                                                                      ATGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGA                         <  SRR4302540.4572597/94‑1 (MQ=60)
                                                                       TGGCCCTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        <  SRR4302540.163640/94‑1 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        <  SRR4302540.9108216/94‑1 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        <  SRR4302540.9108217/94‑1 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        <  SRR4302540.9108215/94‑1 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        >  SRR4302540.7007379/1‑94 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        <  SRR4302540.3862799/94‑1 (MQ=60)
                                                                       TGGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGAC                        >  SRR4302540.622752/1‑94 (MQ=60)
                                                                        GGCACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACT                       <  SRR4302540.766828/94‑1 (MQ=60)
                                                                          GACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCC                     <  SRR4302540.2463850/94‑1 (MQ=60)
                                                                           TCTTCGGTTTGTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCG                    <  SRR4302540.1534759/94‑1 (MQ=60)
                                                                           ACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATNACTCCG                    >  SRR4302540.1391549/1‑94 (MQ=60)
                                                                           ACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCG                    >  SRR4302540.4938618/1‑94 (MQ=60)
                                                                           ACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCG                    <  SRR4302540.3096517/94‑1 (MQ=60)
                                                                           ACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCG                    >  SRR4302540.424556/1‑94 (MQ=60)
                                                                           ACTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCG                    >  SRR4302540.302183/1‑94 (MQ=60)
                                                                            CTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGC                   >  SRR4302540.9825640/1‑94 (MQ=60)
                                                                            CTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGC                   >  SRR4302540.9286443/1‑94 (MQ=60)
                                                                            CTTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGC                   >  SRR4302540.5702739/1‑94 (MQ=60)
                                                                             TTCGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCC                  >  SRR4302540.7378729/1‑94 (MQ=60)
                                                                               CGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAA                >  SRR4302540.1035088/1‑94 (MQ=60)
                                                                               CGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAA                <  SRR4302540.4607873/94‑1 (MQ=60)
                                                                               CGGTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAA                >  SRR4302540.2090126/1‑94 (MQ=60)
                                                                                 GTTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAA              >  SRR4302540.10075524/1‑94 (MQ=60)
                                                                                  TTTTTTAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             <  SRR4302540.972941/94‑1 (MQ=60)
                                                                                  TTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             <  SRR4302540.4116627/94‑1 (MQ=60)
                                                                                  TTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             >  SRR4302540.9851604/1‑94 (MQ=60)
                                                                                  TTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             <  SRR4302540.9784516/94‑1 (MQ=60)
                                                                                  TTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             <  SRR4302540.2198711/94‑1 (MQ=60)
                                                                                  GTTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAAT             <  SRR4302540.2375510/94‑1 (MQ=60)
                                                                                   TTTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATC            >  SRR4302540.4693622/1‑94 (MQ=60)
                                                                                    TTTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCT           <  SRR4302540.8736880/94‑1 (MQ=60)
                                                                                     TTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTA          >  SRR4302540.1534880/1‑94 (MQ=60)
                                                                                     TTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTA          >  SRR4302540.3101096/1‑94 (MQ=60)
                                                                                     TTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTA          >  SRR4302540.3402017/1‑94 (MQ=60)
                                                                                     TTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTA          <  SRR4302540.3557729/94‑1 (MQ=60)
                                                                                     TTGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTA          >  SRR4302540.8976587/1‑94 (MQ=60)
                                                                                      TGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTAT         >  SRR4302540.4260371/1‑94 (MQ=60)
                                                                                      TGAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTAT         >  SRR4302540.4260372/1‑94 (MQ=60)
                                                                                       GAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATC        <  SRR4302540.1235882/94‑1 (MQ=60)
                                                                                       GAAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATC        <  SRR4302540.4327222/94‑1 (MQ=60)
                                                                                        AAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCG       <  SRR4302540.2023848/94‑1 (MQ=60)
                                                                                        AAGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCG       <  SRR4302540.2145854/94‑1 (MQ=60)
                                                                                        AAGGCGTCCGTTGCGACGACTCGCACAAAGGACCGGTTGGATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCG       <  SRR4302540.2145856/94‑1 (MQ=60)
                                                                                         AGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGC      >  SRR4302540.9607692/1‑94 (MQ=60)
                                                                                         AGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGC      <  SRR4302540.9769176/94‑1 (MQ=60)
                                                                                         AGGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGC      >  SRR4302540.4301128/1‑94 (MQ=60)
                                                                                          GGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCT     <  SRR4302540.7165078/94‑1 (MQ=60)
                                                                                          GGCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCT     <  SRR4302540.5188277/94‑1 (MQ=60)
                                                                                           GCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTT    <  SRR4302540.1798393/94‑1 (MQ=60)
                                                                                           GCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTT    <  SRR4302540.566366/94‑1 (MQ=60)
                                                                                           GCGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTT    <  SRR4302540.2768766/94‑1 (MQ=60)
                                                                                            CGTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTC   >  SRR4302540.3671351/1‑94 (MQ=60)
                                                                                             GTCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTCC  <  SRR4302540.4961245/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCATGACTCCGCCAAAATCTATCGCTTCC  >  NC_000913/3950131‑3950317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑