Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,124	+G	intergenic (+58/+26)	ilvC → / ← ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,123	1	.	G	99.3%	787.6 / ‑2.3	271	intergenic (+57/+27)	ilvC/ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (81/187); total (83/188)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

taCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑aa                                                                                           <  1:8483600/92‑1 (MQ=21)

gACAGATATGAAACGTATTGCTTTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑aa                                                                                           <  1:8610907/93‑1 (MQ=21)

 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑g                                                                                         >  1:1612508/1‑93 (MQ=21)

    gATGTGAAACGTATTGCTGTTGCGGTTTAAGTGCGCGCGTATCCCCTCCCCCCGTCC‑‑CTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑ggcc                                                                                      <  2:7245091/93‑4 (MQ=18)

    gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑ggcc                                                                                      <  1:28121/93‑4 (MQ=21)

    gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑ggcc                                                                                      <  2:453109/93‑4 (MQ=21)

     atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑ggcct                                                                                     <  2:3378393/93‑5 (MQ=21)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTtc                                                                                   <  2:431021/93‑1 (MQ=18)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTtc                                                                                   <  2:2213877/93‑1 (MQ=18)

        tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTtct                                                                                  <  2:6938024/93‑1 (MQ=18)

          aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTcc                                                                                <  2:6648728/93‑1 (MQ=18)

           aaCGTATTGCTGTTGCGGGTTAAGCGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCt                                                                               <  1:5855060/93‑1 (MQ=18)

            aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTg                                                                              <  2:5072294/93‑1 (MQ=18)

            aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTg                                                                              <  1:1580200/93‑1 (MQ=18)

               tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCC‑‑ACCCCCTCCCCCAAGGGGG‑GGGAGGAAACAATCC‑AA‑G‑CCTTCTCCTGgag                                                                           >  2:6832818/1‑93 (MQ=16)

                aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGgaga                                                                          <  1:2970114/93‑1 (MQ=18)

                 ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGaa                                                                         <  1:230901/93‑1 (MQ=18)

                 ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGaa                                                                         <  1:945397/93‑1 (MQ=18)

                   gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAgg                                                                       <  2:1713423/93‑1 (MQ=18)

                      gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGC‑AC‑CTCTCCCACAGGGGGA‑GGGAGAAAACACT‑CAAA‑G‑CCTTCTCCTGGAGAAGGGCt                                                                    >  1:6908493/1‑93 (MQ=18)

                      gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCt                                                                    <  1:9612040/93‑1 (MQ=18)

                      gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCt                                                                    <  2:3885648/93‑1 (MQ=18)

                       ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCtt                                                                   <  1:8416769/93‑1 (MQ=18)

                        ttcGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTg                                                                  <  1:4511446/91‑1 (MQ=18)

                        tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTg                                                                  <  1:5937716/93‑1 (MQ=18)

                        tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTg                                                                  <  2:2595174/93‑1 (MQ=18)

                          cGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCt                                                                <  1:4669408/93‑1 (MQ=18)

                            ggTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTAt                                                              <  2:407435/93‑1 (MQ=18)

                            ggTTAAGTGCGCGCTGATGCCCCCCCCCCC‑‑ACCCCCCCCCCCCCGGAGAGGGGGGAAAAAAAC‑A‑AA‑A‑CCCTCTCCTTGAGAAAGCCTTGCTAt                                                              >  2:4859313/1‑93 (MQ=255)

                             gTTAAGTGCGCGCTGATGCCCTCGCCCCGCCC‑‑CTCTCCCACAGGCAGA‑GGGAGAAAGCACT‑C‑AAGG‑TCTTCTCCAGGAGAAGGCCTTGCTAtt                                                             <  2:1119453/93‑1 (MQ=17)

                             gTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTAtt                                                             <  2:9805315/93‑1 (MQ=18)

                             gTTAAGTGCGCGCTGATGCCCTCACCACG‑‑ACCCTCTACCCCGGGGAGA‑GGGAGAACACACT‑C‑AAGG‑CCTTCTCCTGGAGAGGGCCTTGCTAtt                                                             <  2:8954127/93‑1 (MQ=18)

                               tAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                           <  1:545640/93‑1 (MQ=18)

                                aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                                          <  2:8550083/93‑1 (MQ=18)

                                aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                                          <  1:8154696/93‑1 (MQ=18)

                                     cgcgcTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                     <  2:2025143/93‑1 (MQ=21)

                                      gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt                                                    <  1:1024307/93‑1 (MQ=21)

                                        gcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                  <  1:6452811/93‑1 (MQ=21)

                                        gcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                  <  1:9099825/93‑1 (MQ=21)

                                         cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  2:1006398/93‑1 (MQ=21)

                                         cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  1:1512254/93‑1 (MQ=21)

                                         cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  1:3823532/93‑1 (MQ=21)

                                           gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                               <  2:2859566/93‑1 (MQ=25)

                                           gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                               <  2:3625155/93‑1 (MQ=25)

                                             tGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc                                             <  1:4223593/93‑1 (MQ=25)

                                               cccTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                                           <  1:701308/93‑1 (MQ=25)

                                               cccTCACCCCG‑‑ACCCTCCCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                                           <  2:1913954/93‑1 (MQ=25)

                                                ccTCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt                                          <  1:4145219/93‑1 (MQ=25)

                                                  tCACCCCG‑‑ACCCTCTCCTACAGGGGGA‑GGGAGAAAACAAT‑C‑AAGG‑CCTTCTCCTGGAGAAAGCCTTGCTATTAGTTGCGGTTCAGCGCCttca                                        >  2:1313969/1‑91 (MQ=255)

                                                  tCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                        >  1:9302539/1‑93 (MQ=37)

                                                  tCACCCCG‑‑ACCCTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                        >  1:5241201/1‑93 (MQ=37)

                                                           gaccctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 <  1:7993473/89‑1 (MQ=38)

                                                           gaccctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 <  2:9132483/89‑1 (MQ=38)

                                                            accctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                <  2:8336278/90‑1 (MQ=255)

                                                             ccctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                               >  1:5746983/3‑93 (MQ=255)

                                                              cctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  1:6211915/92‑1 (MQ=255)

                                                               ctctCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat                             >  1:2580827/1‑93 (MQ=255)

                                                                tctcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                            <  1:3910405/93‑1 (MQ=255)

                                                                tctcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                            >  1:7270129/1‑93 (MQ=255)

                                                                  tcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          >  2:8115865/1‑93 (MQ=255)

                                                                  tcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          >  2:2786012/1‑93 (MQ=255)

                                                                  tcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          >  2:7923269/1‑93 (MQ=255)

                                                                  tcCCACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          <  1:7832857/93‑1 (MQ=255)

                                                                   cccACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                         <  2:6496041/93‑1 (MQ=255)

                                                                    ccACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGac                        >  1:2879560/1‑92 (MQ=255)

                                                                    ccACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        >  2:6866438/1‑93 (MQ=255)

                                                                    ccACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        <  1:32497/93‑1 (MQ=255)

                                                                    ccACAGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        <  1:9723890/93‑1 (MQ=255)

                                                                     cacaGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                       >  2:5556530/1‑93 (MQ=255)

                                                                     cacaGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                       >  2:9607479/1‑93 (MQ=255)

                                                                      acaGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACt                      <  1:9192982/93‑1 (MQ=255)

                                                                       caGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     >  1:9960060/1‑93 (MQ=255)

                                                                       caGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     <  1:1976285/93‑1 (MQ=255)

                                                                       caGGGAGA‑GGGAGAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCCTGGTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     >  2:473292/1‑93 (MQ=255)

                                                                        agggaga‑gggagAAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                    >  2:946124/1‑93 (MQ=255)

                                                                         gggaga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                   <  1:7383276/93‑1 (MQ=255)

                                                                         gggaga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTCGCCAGTAGTTGCGGGACAGCACCTGTATGTTGTGAGATCCGAACTggg                   >  2:7713568/1‑93 (MQ=255)

                                                                           gaga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtg                 >  1:515729/1‑93 (MQ=255)

                                                                            aga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                <  2:6525414/93‑1 (MQ=255)

                                                                             ga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg               >  1:1943469/1‑93 (MQ=255)

                                                                             ga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg               >  2:7345360/1‑93 (MQ=255)

                                                                             ga‑gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg               >  1:2766461/1‑93 (MQ=255)

                                                                                gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGATAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  2:5285359/93‑1 (MQ=255)

                                                                                gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             >  2:3442585/1‑93 (MQ=255)

                                                                                gggagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  2:3832326/93‑1 (MQ=255)

                                                                                  gagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc           >  2:2891511/1‑93 (MQ=255)

                                                                                  gagaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc           <  1:3106802/93‑1 (MQ=255)

                                                                                   agaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg          >  2:805844/1‑93 (MQ=255)

                                                                                   agaAAACACT‑C‑AAGG‑ACTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg          >  1:2246709/1‑93 (MQ=255)

                                                                                    gaAAACACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg         >  1:3650016/1‑93 (MQ=255)

                                                                                     aaaaCACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg        >  1:7926106/1‑93 (MQ=255)

                                                                                       aaCACT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc      >  2:9667051/1‑93 (MQ=255)

                                                                                        acacT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg     <  2:501141/93‑1 (MQ=255)

                                                                                         cacT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGTACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCTGGCCgg    >  1:4733603/1‑93 (MQ=255)

                                                                                         cacT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg    >  2:6246329/1‑93 (MQ=255)

                                                                                          acT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:3108836/93‑1 (MQ=255)

                                                                                          acT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  1:1325764/93‑1 (MQ=255)

                                                                                           cT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  1:1391337/93‑1 (MQ=255)

                                                                                           cT‑C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  >  2:6131500/1‑93 (MQ=255)

                                                                                           cT‑C‑AA‑GTCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  2:699131/93‑1 (MQ=255)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGA‑GGGAGAAAACACT‑C‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC  >  NC_000913/3957033‑3957216

gaaagataggacacggatggctggTGCGGGTTAAGGGCGCGCGTATGCCCTCACCCCGTCC‑‑CTCCCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              <  SRR4302540.4291592/70‑1 (MQ=1)

TACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              <  SRR4302540.8582148/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTTTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              <  SRR4302540.8710961/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              <  SRR4302540.746032/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              >  SRR4302540.1630176/1‑94 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                              <  SRR4302540.6924463/94‑1 (MQ=60)

   AGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCCCAGGGAGAGGGAGAAAACTCTC‑AA‑Ggcc                                                                                           <  SRR4302540.2934193/94‑4 (MQ=60)

    GATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCT                                                                                           <  SRR4302540.4606184/94‑1 (MQ=60)

    GATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCT                                                                                           <  SRR4302540.28421/94‑1 (MQ=60)

      TATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTC                                                                                         <  SRR4302540.582151/94‑1 (MQ=60)

       ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                                                        <  SRR4302540.8059311/94‑1 (MQ=60)

       ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCT                                                                                        <  SRR4302540.289467/94‑1 (MQ=60)

        TGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTC                                                                                       <  SRR4302540.4802426/94‑1 (MQ=60)

         GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCC                                                                                      <  SRR4302540.794156/94‑1 (MQ=60)

          AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCT                                                                                     <  SRR4302540.7872068/94‑1 (MQ=60)

           AACGTATTGCTGTTGCGGGTTAAGCGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTG                                                                                    <  SRR4302540.5919812/94‑1 (MQ=54)

            ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGG                                                                                   <  SRR4302540.2700468/94‑1 (MQ=60)

            ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGG                                                                                   <  SRR4302540.1597467/94‑1 (MQ=60)

              CTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAG                                                                                 <  SRR4302540.160593/94‑1 (MQ=47)

                ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAA                                                                               <  SRR4302540.143073/94‑1 (MQ=60)

                ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAA                                                                               <  SRR4302540.3002915/94‑1 (MQ=60)

                 gtgccGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCCCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                                                              <  SRR4302540.8057451/89‑1 (MQ=37)

                 TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                                                              <  SRR4302540.955257/94‑1 (MQ=60)

                 TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                                                              <  SRR4302540.233132/94‑1 (MQ=60)

                 TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAG                                                                              <  SRR4302540.3850544/94‑1 (MQ=60)

                     TGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCAC‑‑CTCTCCCACAGGGGGAGGGAGAAAACACTCAAA‑GCCTTCTCCTGGAGAAGGGCT                                                                          >  SRR4302540.6985340/1‑94 (MQ=50)

                      GTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTT                                                                         <  SRR4302540.8685931/94‑1 (MQ=60)

                      GTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTT                                                                         <  SRR4302540.9725373/94‑1 (MQ=60)

                      GTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTT                                                                         <  SRR4302540.2283212/94‑1 (MQ=60)

                       TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTG                                                                        <  SRR4302540.8514467/94‑1 (MQ=60)

                       TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTG                                                                        <  SRR4302540.1892540/94‑1 (MQ=60)

                       TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTG                                                                        <  SRR4302540.3908248/94‑1 (MQ=60)

                        TTCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGC                                                                       <  SRR4302540.4560326/94‑1 (MQ=57)

                        TGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGC                                                                       <  SRR4302540.6003354/94‑1 (MQ=60)

                          CGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTA                                                                     <  SRR4302540.4719385/94‑1 (MQ=60)

                             GTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTA                                                                  <  SRR4302540.2224762/94‑1 (MQ=60)

                             GTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTA                                                                  <  SRR4302540.9396472/94‑1 (MQ=60)

                             GTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTA                                                                  <  SRR4302540.5931695/94‑1 (MQ=60)

                               TAAGTGCGCGCTTATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACGC‑AAGGCCTTGTCCTGGAGAAGGCCTTGCTATTAGT                                                                <  SRR4302540.3872288/94‑1 (MQ=35)

                               TAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGT                                                                <  SRR4302540.551578/94‑1 (MQ=60)

                               TAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGT                                                                <  SRR4302540.6022734/94‑1 (MQ=60)

                                AAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTT                                                               <  SRR4302540.8249376/94‑1 (MQ=60)

                                AAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTT                                                               <  SRR4302540.6047721/94‑1 (MQ=60)

                                 AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                                                              <  SRR4302540.1318445/94‑1 (MQ=60)

                                 AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                                                              <  SRR4302540.85655/94‑1 (MQ=60)

                                  GTGCGCGCTGTTGCCGTCACCCCG‑‑ACCCTCCCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGC                                                             <  SRR4302540.7399572/94‑1 (MQ=55)

                                   TGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCG                                                            <  SRR4302540.5145862/94‑1 (MQ=60)

                                    GCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                                                           <  SRR4302540.8247787/94‑1 (MQ=60)

                                    GCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                                                           <  SRR4302540.1972461/94‑1 (MQ=54)

                                      GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                                                         <  SRR4302540.1034698/94‑1 (MQ=60)

                                      GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                                                         <  SRR4302540.7145994/94‑1 (MQ=60)

                                       CGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAC                                                        <  SRR4302540.5878497/94‑1 (MQ=60)

                                        GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                                                       <  SRR4302540.6524628/94‑1 (MQ=60)

                                        GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                                                       <  SRR4302540.6524629/94‑1 (MQ=60)

                                        GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                                                       <  SRR4302540.9209166/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                      <  SRR4302540.3404589/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                      <  SRR4302540.1528961/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                      <  SRR4302540.3865140/94‑1 (MQ=60)

                                          TGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGATAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                                                     <  SRR4302540.4574431/94‑1 (MQ=60)

                                          TGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                                                     <  SRR4302540.9469577/94‑1 (MQ=60)

                                          TGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                                                     <  SRR4302540.9265550/94‑1 (MQ=60)

                                             TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                                                  <  SRR4302540.4269565/94‑1 (MQ=60)

                                             TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                                                  <  SRR4302540.4269564/94‑1 (MQ=60)

                                               ggcctgcaccccgaccCTCTCCCATAGGTAGAGGGAGAAAACACTC‑AAGGCCGTCTCCTGGAGAAGGCCTGGCTATTAGTTGCGGTACAGCACC                                                  <  SRR4302540.945091/78‑1 (MQ=60)

                                               CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                                                <  SRR4302540.708836/94‑1 (MQ=60)

                                                CCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAACCACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA                                               <  SRR4302540.2820598/94‑1 (MQ=60)

                                                CCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA                                               <  SRR4302540.4190080/94‑1 (MQ=60)

                                                CCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA                                               <  SRR4302540.8933682/94‑1 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTTCCGCCGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCCATTAGTTGgcgacaaggaccttat                                              >  SRR4302540.2870724/1‑78 (MQ=47)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                              >  SRR4302540.2870725/1‑94 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                              >  SRR4302540.9413650/1‑94 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                              <  SRR4302540.2039036/94‑1 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                              >  SRR4302540.5298374/1‑94 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGATAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                             <  SRR4302540.9824922/94‑1 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                             >  SRR4302540.5414870/1‑94 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                             <  SRR4302540.9824923/94‑1 (MQ=60)

                                                  CCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                             <  SRR4302540.261104/94‑1 (MQ=60)

                                                   ctcccccccgcccctcCCCACAGGGAGAAGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                              <  SRR4302540.8152414/78‑1 (MQ=60)

                                                    ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                           >  SRR4302540.5962373/1‑94 (MQ=60)

                                                    ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                           >  SRR4302540.8735360/1‑94 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                          <  SRR4302540.2021802/94‑1 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                          <  SRR4302540.1400275/94‑1 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                          <  SRR4302540.2101004/94‑1 (MQ=60)

                                                      cgcccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                           <  SRR4302540.3371614/85‑1 (MQ=60)

                                                      CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                         >  SRR4302540.3104861/1‑94 (MQ=60)

                                                      CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                         >  SRR4302540.1600082/1‑94 (MQ=60)

                                                      CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                         <  SRR4302540.6247384/94‑1 (MQ=60)

                                                       ccccgcccccccccagCAGGGAGAGCGAGACACCACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                          <  SRR4302540.3758635/78‑1 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                        <  SRR4302540.2902354/94‑1 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                        <  SRR4302540.1420898/94‑1 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                        <  SRR4302540.8565070/94‑1 (MQ=60)

                                                          cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                       <  SRR4302540.9319759/89‑1 (MQ=60)

                                                          cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                       <  SRR4302540.7526661/89‑1 (MQ=60)

                                                          cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                       >  SRR4302540.8475224/6‑94 (MQ=60)

                                                           gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                      <  SRR4302540.8086528/90‑1 (MQ=60)

                                                            cccCTCTCCCCCAGGGAGAGGGAGAAAACCCTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                     <  SRR4302540.3735247/91‑1 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                     >  SRR4302540.5810371/4‑94 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   <  SRR4302540.6281038/94‑1 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   >  SRR4302540.1591124/1‑94 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   <  SRR4302540.9690718/94‑1 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   >  SRR4302540.5890874/1‑94 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   >  SRR4302540.9238679/1‑94 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                   >  SRR4302540.2608652/1‑94 (MQ=60)

                                                             ccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATA                                    <  SRR4302540.6419153/92‑1 (MQ=60)

                                                             ccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATA                                    >  SRR4302540.5540184/3‑94 (MQ=60)

                                                             ccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATA                                    >  SRR4302540.3014984/3‑94 (MQ=60)

                                                             ccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATA                                    <  SRR4302540.2961826/92‑1 (MQ=60)

                                                             ccCTCTCACACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATA                                    <  SRR4302540.425005/92‑1 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTACTGATGTGATATC                                  >  SRR4302540.5589608/1‑94 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                  >  SRR4302540.477940/1‑94 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                  >  SRR4302540.7351254/1‑94 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                  <  SRR4302540.10037038/94‑1 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                  >  SRR4302540.10042074/1‑94 (MQ=60)

                                                                TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                 <  SRR4302540.3952779/94‑1 (MQ=60)

                                                                CCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                 <  SRR4302540.3179183/94‑1 (MQ=60)

                                                                CCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                 <  SRR4302540.8164475/94‑1 (MQ=60)

                                                                 CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCG                                >  SRR4302540.1605331/1‑94 (MQ=60)

                                                                  TCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                               <  SRR4302540.7924380/94‑1 (MQ=60)

                                                                  TCCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                               <  SRR4302540.7924383/94‑1 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAC                              >  SRR4302540.2910880/1‑94 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAA                              >  SRR4302540.1343453/1‑94 (MQ=60)

                                                                    CCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                             <  SRR4302540.9838107/94‑1 (MQ=60)

                                                                    CCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                             <  SRR4302540.32843/94‑1 (MQ=60)

                                                                    CCACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                             <  SRR4302540.5409973/94‑1 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                            >  SRR4302540.6787028/1‑94 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                            <  SRR4302540.1291924/94‑1 (MQ=60)

                                                                      ACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTG                           >  SRR4302540.10076287/1‑94 (MQ=60)

                                                                      ACAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTG                           <  SRR4302540.9303242/94‑1 (MQ=60)

                                                                       CAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGACCTGG                          >  SRR4302540.1970500/1‑94 (MQ=60)

                                                                       CAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGG                          <  SRR4302540.1997724/94‑1 (MQ=60)

                                                                       CAGGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGG                          >  SRR4302540.7041192/1‑94 (MQ=60)

                                                                         GGGAGAGGGTGAAAACACTC‑AATGCCTTCTCCTGGATTAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAGGTGATATCCGAACTGGGG                        >  SRR4302540.9027666/1‑94 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                        >  SRR4302540.5925694/1‑94 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                        <  SRR4302540.7465541/94‑1 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                        <  SRR4302540.9711678/94‑1 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                        <  SRR4302540.1380820/94‑1 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                       >  SRR4302540.521259/1‑94 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                       >  SRR4302540.4452382/1‑94 (MQ=60)

                                                                           GAGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGT                      <  SRR4302540.2811345/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     >  SRR4302540.1538592/1‑94 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     >  SRR4302540.1964627/1‑94 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     >  SRR4302540.2796351/1‑94 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     <  SRR4302540.3234904/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     <  SRR4302540.4734264/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     <  SRR4302540.5269434/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                     <  SRR4302540.5269435/94‑1 (MQ=60)

                                                                             GAGGGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGC                    >  SRR4302540.2415448/1‑94 (MQ=60)

                                                                                GGAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGC                 <  SRR4302540.2382904/94‑1 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                >  SRR4302540.2270846/1‑94 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                <  SRR4302540.3140809/94‑1 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                >  SRR4302540.185501/1‑94 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGGACTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                >  SRR4302540.2270845/1‑94 (MQ=60)

                                                                                  AGAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG               >  SRR4302540.3690003/1‑94 (MQ=60)

                                                                                   GAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG              <  SRR4302540.4021995/94‑1 (MQ=60)

                                                                                   GAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG              >  SRR4302540.9446712/1‑94 (MQ=60)

                                                                                   GAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG              <  SRR4302540.8374344/94‑1 (MQ=60)

                                                                                   GAAAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG              >  SRR4302540.8018491/1‑94 (MQ=60)

                                                                                     AAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC            >  SRR4302540.4064817/1‑94 (MQ=60)

                                                                                     AAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC            <  SRR4302540.9407432/94‑1 (MQ=60)

                                                                                     AAACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC            <  SRR4302540.3597136/94‑1 (MQ=60)

                                                                                      AACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCG           >  SRR4302540.713731/1‑94 (MQ=60)

                                                                                      AACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCG           >  SRR4302540.3084619/1‑94 (MQ=60)

                                                                                       ACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGTACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCTGGCCGG          >  SRR4302540.4784148/1‑94 (MQ=60)

                                                                                       ACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG          >  SRR4302540.4326097/1‑94 (MQ=60)

                                                                                       ACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG          <  SRR4302540.8543464/94‑1 (MQ=60)

                                                                                       ACACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG          >  SRR4302540.7582208/1‑94 (MQ=60)

                                                                                         ACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGTACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC        >  SRR4302540.8626005/1‑94 (MQ=60)

                                                                                         ACTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC        <  SRR4302540.1339594/94‑1 (MQ=60)

                                                                                          CTC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG       <  SRR4302540.1406231/94‑1 (MQ=60)

                                                                                           TC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGG      >  SRR4302540.6990083/1‑94 (MQ=60)

                                                                                           TC‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGG      <  SRR4302540.7232127/94‑1 (MQ=60)

                                                                                              aagGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCT    <  SRR4302540.2350783/91‑1 (MQ=60)

                                                                                              AG‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTC   <  SRR4302540.8305904/94‑1 (MQ=60)

                                                                                               G‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC  <  SRR4302540.5375157/94‑1 (MQ=60)

                                                                                               G‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC  <  SRR4302540.3623586/94‑1 (MQ=60)

                                                                                               G‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC  >  SRR4302540.2721902/1‑94 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC  >  NC_000913/3957033‑3957216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`