Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,124	+G	intergenic (+58/+26)	ilvC → / ← ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,123	1	.	G	100.0%	463.1 / NA	156	intergenic (+57/+27)	ilvC/ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (49/107); total (49/107)

Only 100 of 179 total aligned reads displayed.

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑aa                                                                                          <  2:690613/93‑1 (MQ=21)

 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑g                                                                                        <  2:3025173/93‑1 (MQ=21)

 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑g                                                                                        <  2:141965/93‑1 (MQ=21)

 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑g                                                                                        <  2:1503394/93‑1 (MQ=21)

 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑g                                                                                        <  1:2148934/93‑1 (MQ=21)

  cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑gg                                                                                       <  2:5427520/93‑2 (MQ=21)

   aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑ggc                                                                                      <  2:5546462/93‑3 (MQ=21)

    aatatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑ggcc                                                                                     <  1:3879865/92‑4 (MQ=18)

     atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑ggcct                                                                                    <  2:3063748/93‑5 (MQ=21)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtc                                                                                   <  2:5698581/93‑1 (MQ=18)

         gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCCTCTCCCCCAGGGAGAGGGAGAAAACACT‑CAAA‑GCCTtctc                                                                                 >  2:2023207/1‑93 (MQ=18)

           aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCt                                                                               <  2:4530529/93‑1 (MQ=18)

            aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTg                                                                              <  1:5029976/93‑1 (MQ=18)

                aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCGCCC‑‑CCCTCCCCCCGGGGGGGGGGGGAAAAACTAC‑AA‑GCCTTCTCCTggggg                                                                          >  2:4141417/1‑90 (MQ=12)

                 ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGaa                                                                         <  1:4267159/93‑1 (MQ=18)

                   gCTGTTGCGGTTTAAGTGCGCGCTGATCCCCTCGCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAgg                                                                       <  2:3298025/93‑1 (MQ=18)

                   gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCCCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAgg                                                                       <  2:3574081/93‑1 (MQ=18)

                      gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCt                                                                    <  2:4562940/93‑1 (MQ=18)

                       ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCtt                                                                   <  2:6101429/93‑1 (MQ=18)

                       gtGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCtt                                                                   <  1:605021/92‑1 (MQ=18)

                         gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                 >  1:3193077/1‑93 (MQ=18)

                          cGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                <  2:24059/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  1:4844363/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGACCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  1:2441000/93‑1 (MQ=18)

                                aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                                          <  1:970136/93‑1 (MQ=18)

                                   tGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                       <  1:6160169/93‑1 (MQ=21)

                                   tGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                       <  1:2513177/93‑1 (MQ=21)

                                    gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                      <  2:5962141/93‑1 (MQ=21)

                                    gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                      <  2:5962140/93‑1 (MQ=21)

                                    gcgcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                      <  2:4919538/93‑1 (MQ=21)

                                      gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt                                                    <  2:4504717/93‑1 (MQ=21)

                                      gcgcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt                                                    <  1:489710/93‑1 (MQ=21)

                                        gcTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                  <  2:23900/93‑1 (MQ=21)

                                         cggATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACGCT‑C‑AAGGCCTCCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  2:3487822/91‑1 (MQ=21)

                                         cTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  2:316080/93‑1 (MQ=21)

                                           gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                               <  2:508328/93‑1 (MQ=25)

                                           gATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                               <  1:931863/93‑1 (MQ=25)

                                                  tCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                        >  1:4988930/1‑93 (MQ=37)

                                                   cACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       <  1:3932169/93‑1 (MQ=37)

                                                   cACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       >  2:4190781/1‑93 (MQ=37)

                                                   cACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       <  1:1871355/93‑1 (MQ=37)

                                                   cACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       <  1:6144739/93‑1 (MQ=37)

                                                   cACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       <  2:4788886/93‑1 (MQ=37)

                                                           gaccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 >  1:5262220/5‑93 (MQ=38)

                                                           gaccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 >  1:3184123/5‑93 (MQ=38)

                                                           gaccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 >  1:3492566/5‑93 (MQ=38)

                                                            accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                >  1:1783621/4‑93 (MQ=255)

                                                            accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                >  2:649494/4‑93 (MQ=255)

                                                            accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                >  2:4425212/4‑93 (MQ=255)

                                                            accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCATTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                <  1:3629304/90‑1 (MQ=38)

                                                             ccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                               >  1:1700290/3‑93 (MQ=255)

                                                             ccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                               <  2:287597/91‑1 (MQ=255)

                                                              cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  1:2480494/92‑1 (MQ=255)

                                                              cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  2:713546/92‑1 (MQ=255)

                                                              cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  1:1072943/92‑1 (MQ=255)

                                                              cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  1:5187503/92‑1 (MQ=255)

                                                              cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                              <  2:6246515/92‑1 (MQ=255)

                                                               ctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat                             >  2:1660254/1‑93 (MQ=255)

                                                                tctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                            >  1:4569390/1‑93 (MQ=255)

                                                                 ctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                           <  1:3255995/93‑1 (MQ=255)

                                                                  tcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          <  1:2818139/93‑1 (MQ=255)

                                                                   cccACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                         >  2:4852188/1‑93 (MQ=255)

                                                                   cccACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                         >  2:4008642/1‑93 (MQ=255)

                                                                    ccACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        <  1:475427/93‑1 (MQ=255)

                                                                      acaGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACt                      <  2:947584/93‑1 (MQ=255)

                                                                       caGTGAGAGGGAGAAAACAGT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     <  2:5425364/93‑1 (MQ=255)

                                                                         gggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                   >  2:6157712/1‑93 (MQ=255)

                                                                         gggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                   >  1:1501492/1‑93 (MQ=255)

                                                                         gggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                   <  2:2569035/93‑1 (MQ=255)

                                                                          ggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgggg                  >  1:4121628/1‑92 (MQ=255)

                                                                          ggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                  >  2:650873/1‑93 (MQ=255)

                                                                          ggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                  <  1:278375/93‑1 (MQ=255)

                                                                           gagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtg                 <  2:5256165/93‑1 (MQ=255)

                                                                            agagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                >  1:4056228/1‑93 (MQ=255)

                                                                             gagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg               <  1:2978751/93‑1 (MQ=255)

                                                                              agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc              <  2:3790039/93‑1 (MQ=255)

                                                                              agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc              <  1:4659967/93‑1 (MQ=255)

                                                                              agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc              <  1:6106212/93‑1 (MQ=255)

                                                                               gggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  1:1583707/93‑1 (MQ=255)

                                                                               gggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  1:932054/93‑1 (MQ=255)

                                                                                ggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg            >  2:2788794/1‑93 (MQ=255)

                                                                                ggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg            >  1:3825973/1‑93 (MQ=255)

                                                                                  agaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg          >  1:4508582/1‑93 (MQ=255)

                                                                                   gaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg         <  1:3457085/93‑1 (MQ=255)

                                                                                   gaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg         >  2:5641366/1‑93 (MQ=255)

                                                                                     aaaCACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc       >  2:3198379/1‑93 (MQ=255)

                                                                                      aaCACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGCGTGCAGCGGGcc      >  1:1549338/1‑93 (MQ=255)

                                                                                      aaCACT‑C‑AAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc      <  1:4837870/93‑1 (MQ=255)

                                                                                       acacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg     <  2:4689914/93‑1 (MQ=255)

                                                                                       acacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg     >  2:1385127/1‑93 (MQ=255)

                                                                                       acacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg     >  2:1691381/1‑93 (MQ=255)

                                                                                       acacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg     <  1:476131/93‑1 (MQ=255)

                                                                                        cacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg    >  1:5406427/1‑93 (MQ=255)

                                                                                         ccT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:3949211/92‑1 (MQ=255)

                                                                                         acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  1:6257602/93‑1 (MQ=255)

                                                                                         acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  1:3393835/93‑1 (MQ=255)

                                                                                         acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:1776100/93‑1 (MQ=255)

                                                                                         acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:2654866/93‑1 (MQ=255)

                                                                                          cT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  1:2565904/93‑1 (MQ=255)

                                                                                          cT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  1:5620256/93‑1 (MQ=255)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  >  NC_000913/3957034‑3957217

ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑GG                                                                                              <  SRR4302533.2162167/94‑1 (MQ=60)

  AGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCT‑‑ACCCTCTTCCACATGGGGGGGGAGAAAACACTCAACGCC                                                                                             >  SRR4302533.3206570/1‑94 (MQ=60)

  AGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑Ggcc                                                                                            <  SRR4302533.3257113/94‑4 (MQ=60)

   AATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCT                                                                                            <  SRR4302533.3903539/94‑1 (MQ=57)

     TATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTC                                                                                          <  SRR4302533.446095/94‑1 (MQ=60)

     TATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTC                                                                                          <  SRR4302533.4428940/94‑1 (MQ=60)

         AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCT                                                                                      <  SRR4302533.2474103/94‑1 (MQ=60)

           ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGG                                                                                    <  SRR4302533.5060430/94‑1 (MQ=60)

           ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGG                                                                                    <  SRR4302533.4786405/94‑1 (MQ=60)

            CGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGA                                                                                   <  SRR4302533.4519621/94‑1 (MQ=60)

             GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAG                                                                                  <  SRR4302533.573364/94‑1 (MQ=60)

               ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAA                                                                                <  SRR4302533.4952138/94‑1 (MQ=60)

               ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAA                                                                                <  SRR4302533.1881633/94‑1 (MQ=60)

                TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAG                                                                               <  SRR4302533.4292854/94‑1 (MQ=60)

                 TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGG                                                                              <  SRR4302533.654281/94‑1 (MQ=60)

                      TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTG                                                                         <  SRR4302533.2746408/94‑1 (MQ=60)

                      GTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTG                                                                         <  SRR4302533.608574/94‑1 (MQ=60)

                       TGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGC                                                                        >  SRR4302533.3212719/1‑94 (MQ=60)

                        GCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCT                                                                       <  SRR4302533.1579772/94‑1 (MQ=60)

                             TTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAG                                                                  <  SRR4302533.4873726/94‑1 (MQ=60)

                             TTAAGTGCGCGCTGATGACCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAG                                                                  <  SRR4302533.2456070/94‑1 (MQ=60)

                              TAAGTGCGCGCTGATGCCCTCGCCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGT                                                                 <  SRR4302533.4751955/94‑1 (MQ=47)

                              TAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGT                                                                 <  SRR4302533.4055625/94‑1 (MQ=60)

                               AAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTT                                                                <  SRR4302533.975826/94‑1 (MQ=60)

                                AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                                                               <  SRR4302533.3338306/94‑1 (MQ=60)

                                  TGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCG                                                             <  SRR4302533.2528663/94‑1 (MQ=60)

                                  TGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCG                                                             <  SRR4302533.6197216/94‑1 (MQ=60)

                                    CGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGT                                                           <  SRR4302533.6191728/94‑1 (MQ=60)

                                     GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                                                          <  SRR4302533.492639/94‑1 (MQ=60)

                                         TGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                                                      <  SRR4302533.464974/94‑1 (MQ=60)

                                          GATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCA                                                     <  SRR4302533.94186/94‑1 (MQ=60)

                                          GATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCA                                                     <  SRR4302533.937333/94‑1 (MQ=60)

                                              CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                                                 <  SRR4302533.6275096/94‑1 (MQ=60)

                                              CCCGCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                                                 <  SRR4302533.4910186/94‑1 (MQ=60)

                                                CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                               >  SRR4302533.5019177/1‑94 (MQ=60)

                                                  CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG                                             <  SRR4302533.3956174/94‑1 (MQ=60)

                                                  CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG                                             <  SRR4302533.1882763/94‑1 (MQ=60)

                                                  CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG                                             <  SRR4302533.6181737/94‑1 (MQ=60)

                                                  CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG                                             <  SRR4302533.2866709/94‑1 (MQ=60)

                                                   ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            >  SRR4302533.1646968/1‑94 (MQ=60)

                                                   ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            >  SRR4302533.5046410/1‑94 (MQ=60)

                                                    CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           >  SRR4302533.6105161/1‑94 (MQ=60)

                                                    CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           >  SRR4302533.2014767/1‑94 (MQ=60)

                                                    CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           >  SRR4302533.2014768/1‑94 (MQ=60)

                                                     CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                          >  SRR4302533.3302120/1‑94 (MQ=60)

                                                         cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                        >  SRR4302533.3203709/6‑94 (MQ=60)

                                                         cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                        >  SRR4302533.3514102/6‑94 (MQ=60)

                                                         cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                        >  SRR4302533.5293809/6‑94 (MQ=60)

                                                          gaccCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                       >  SRR4302533.1794468/5‑94 (MQ=60)

                                                          gaccCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                       >  SRR4302533.5190505/5‑94 (MQ=60)

                                                           accCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      >  SRR4302533.1710595/4‑94 (MQ=60)

                                                           accCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCATTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      <  SRR4302533.3651635/91‑1 (MQ=60)

                                                           C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                    <  SRR4302533.5218813/94‑1 (MQ=60)

                                                           C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                    <  SRR4302533.2495791/94‑1 (MQ=60)

                                                           C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                    <  SRR4302533.1079270/94‑1 (MQ=60)

                                                              CTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                   >  SRR4302533.4597081/1‑94 (MQ=60)

                                                               TCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                  <  SRR4302533.5029944/94‑1 (MQ=60)

                                                                CTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCG                                 <  SRR4302533.3276073/94‑1 (MQ=60)

                                                                 TCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                                <  SRR4302533.2835483/94‑1 (MQ=60)

                                                                   CCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                              >  SRR4302533.5330758/1‑94 (MQ=60)

                                                                   CCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                              <  SRR4302533.478265/94‑1 (MQ=60)

                                                                    CACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             <  SRR4302533.2531081/94‑1 (MQ=60)

                                                                     ACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTG                            <  SRR4302533.2007028/94‑1 (MQ=60)

                                                                       AGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGG                          >  SRR4302533.1510542/1‑94 (MQ=60)

                                                                       AGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGG                          >  SRR4302533.4153640/1‑94 (MQ=60)

                                                                        GGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGG                         >  SRR4302533.4146652/1‑94 (MQ=60)

                                                                         GGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGTACTGGGTG                        >  SRR4302533.2814096/1‑94 (MQ=60)

                                                                         GGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                        <  SRR4302533.280073/94‑1 (MQ=60)

                                                                          GAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGT                       >  SRR4302533.4080921/1‑94 (MQ=60)

                                                                            GAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGC                     <  SRR4302533.2996975/94‑1 (MQ=60)

                                                                             AGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    >  SRR4302533.2255975/1‑94 (MQ=60)

                                                                             AGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    <  SRR4302533.6143083/94‑1 (MQ=60)

                                                                             AGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    <  SRR4302533.4688208/94‑1 (MQ=60)

                                                                              GGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   >  SRR4302533.3849362/1‑94 (MQ=60)

                                                                              GGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302533.1593251/94‑1 (MQ=60)

                                                                              GGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302533.937526/94‑1 (MQ=60)

                                                                              GGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302533.3559799/94‑1 (MQ=60)

                                                                                GAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                 >  SRR4302533.4535887/1‑94 (MQ=60)

                                                                                GAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                 <  SRR4302533.4114656/94‑1 (MQ=60)

                                                                                 AGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                >  SRR4302533.1645447/1‑94 (MQ=60)

                                                                                  GAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCTGAACTGGGTGTGCAGCGGG               >  SRR4302533.3672109/1‑94 (MQ=60)

                                                                                  GAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               >  SRR4302533.6168253/1‑94 (MQ=60)

                                                                                  GAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               >  SRR4302533.3875258/1‑94 (MQ=60)

                                                                                  GAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               <  SRR4302533.3478429/94‑1 (MQ=60)

                                                                                    AAACACTCAAGGCCTTCTCCTGGAGAAGGCGTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC             <  SRR4302533.153465/94‑1 (MQ=60)

                                                                                    AAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGCGTGCAGCGGGCC             >  SRR4302533.1558677/1‑94 (MQ=60)

                                                                                     AACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCG            <  SRR4302533.3458223/94‑1 (MQ=60)

                                                                                     AACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCG            <  SRR4302533.4867192/94‑1 (MQ=60)

                                                                                      ACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           >  SRR4302533.5438915/1‑94 (MQ=60)

                                                                                      ACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           <  SRR4302533.478973/94‑1 (MQ=60)

                                                                                      ACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           <  SRR4302533.485281/94‑1 (MQ=60)

                                                                                        ACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302533.3414795/94‑1 (MQ=60)

                                                                                        ACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302533.6295024/94‑1 (MQ=60)

                                                                                         CTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        >  SRR4302533.1585461/1‑94 (MQ=60)

                                                                                         CTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302533.2581694/94‑1 (MQ=60)

                                                                                         CTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302533.5654225/94‑1 (MQ=60)

                                                                                         CTCAAGGCCTTCTCCTGGAGAAGGCCTGGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302533.558662/94‑1 (MQ=60)

                                                                                          TCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGG       <  SRR4302533.5798040/94‑1 (MQ=60)

                                                                                          TCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGG       >  SRR4302533.4823598/1‑94 (MQ=60)

                                                                                            aagGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCT     >  SRR4302533.2624930/4‑94 (MQ=60)

                                                                                            aagGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCT     >  SRR4302533.3934107/4‑94 (MQ=60)

                                                                                            aagGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCT     >  SRR4302533.3327781/4‑94 (MQ=60)

                                                                                               GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  >  SRR4302533.4118694/1‑94 (MQ=60)

                                                                                               GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  <  SRR4302533.1065507/94‑1 (MQ=60)

ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTCAA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  >  NC_000913/3957034‑3957217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`