Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,491,023 A→G Y318H (TAT→CAT)  idnD ← L‑idonate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,0230AG93.6% 1173.2 / 52.2 420Y318H (TAT→CAT) idnDL‑idonate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (16/11);  new base G (154/239);  total (170/250)
Fisher's exact test for biased strand distribution p-value = 4.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 447 total aligned reads displayed.

TTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC  >  NC_000913/4490932‑4491112
                                                                                           |                                                                                         
 tatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGtt                                                                                         >  2:1806344/1‑93 (MQ=255)
 tatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGtt                                                                                         <  1:9227351/93‑1 (MQ=255)
  atttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTc                                                                                        <  2:4310694/93‑1 (MQ=255)
   tttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCa                                                                                       >  1:6281147/1‑93 (MQ=255)
     tatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGc                                                                                     >  2:4713567/1‑93 (MQ=255)
      atCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCa                                                                                    <  1:4606584/93‑1 (MQ=255)
       tctcTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAc                                                                                   >  2:1174811/1‑93 (MQ=255)
       tctcTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAc                                                                                   <  1:2938501/93‑1 (MQ=255)
          ctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCTCTCa                                                                                <  2:159914/93‑1 (MQ=255)
          ctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCa                                                                                <  2:6720227/93‑1 (MQ=255)
           ttAGAAAGCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAg                                                                               >  2:9013585/1‑93 (MQ=255)
           ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAg                                                                               >  1:9832701/1‑93 (MQ=255)
             aGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTa                                                                             >  1:6238462/1‑93 (MQ=255)
             aGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTa                                                                             <  1:7339429/93‑1 (MQ=255)
              gAAAACAAGCTGGACTTTTGCTGCCCGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaa                                                                            >  1:7554428/1‑93 (MQ=255)
               aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaaa                                                                           <  2:9579543/93‑1 (MQ=255)
               aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaaa                                                                           <  2:8753729/93‑1 (MQ=255)
               aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaaa                                                                           <  1:7047748/93‑1 (MQ=255)
               aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCCGGCAAACCTCGCGCCTTTTCCACGTCAGCGAGGGGATGCTCCGCACTCAGTaac                                                                           >  1:3449469/1‑92 (MQ=255)
                aaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAg                                                                          >  2:511968/1‑93 (MQ=255)
                 aaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAgg                                                                         >  2:4592359/1‑93 (MQ=255)
                 aaCAAGCTGGACTTTTGCTGCCTGGGGTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGAGGTTCAGCACTCAGTaaacg                                                                         >  1:1355197/1‑91 (MQ=255)
                  aCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGc                                                                        <  1:8900440/93‑1 (MQ=255)
                  aCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGc                                                                        >  2:439852/1‑93 (MQ=255)
                   cAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCa                                                                       <  1:10002172/93‑1 (MQ=255)
                    aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      >  1:5135302/1‑93 (MQ=255)
                     aGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAgg                                                                     >  2:8113121/1‑92 (MQ=255)
                     aGCTGGACTTTTGCTGCCTGGGATTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCCGTGAAGGGTTGTTCAGCACTCAGTAAAGGCAgg                                                                     >  2:659389/1‑92 (MQ=255)
                      acTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAgag                                                                    <  1:8531948/92‑3 (MQ=255)
                       cgggACTTTTGCTGCCTGGGTTTTACACCCGGCGAACCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTgg                                                                   <  1:865686/91‑1 (MQ=255)
                        tGGACTTTTGCTGCCTGGTTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGa                                                                  <  2:72075/93‑1 (MQ=255)
                        tGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGa                                                                  >  1:1222331/1‑93 (MQ=255)
                        tGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGa                                                                  <  2:2549096/93‑1 (MQ=255)
                         ggACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAt                                                                 <  2:7753396/93‑1 (MQ=255)
                          gACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAtt                                                                <  2:2206975/93‑1 (MQ=255)
                            cgtgtgctgcCTGGGTTTTAGCACCGGCGAAAGGTAGCGCCTCGTCCAGGTCAGTGAAGGGATATTCAGCACACAGTAAAGGAAGTGGATTGa                                                              <  2:7058320/89‑1 (MQ=255)
                             ttttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAt                                                             <  1:947034/93‑1 (MQ=255)
                              tttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATa                                                            <  1:3905907/93‑1 (MQ=255)
                               ttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATaa                                                           <  2:5956560/93‑1 (MQ=255)
                               ttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATaa                                                           <  2:2119559/93‑1 (MQ=255)
                                tgctgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAc                                                          <  2:8371140/93‑1 (MQ=255)
                                  ctgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGc                                                        <  1:2266851/93‑1 (MQ=255)
                                    gcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCa                                                      <  2:3365356/93‑1 (MQ=255)
                                     ccTGGGTTTTATCACCGGCGAAACGTAGCTCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCAt                                                     <  1:4822449/93‑1 (MQ=255)
                                     ccTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCAt                                                     <  1:4186767/93‑1 (MQ=255)
                                        gggTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCg                                                  >  2:2944199/1‑93 (MQ=255)
                                         ggTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc                                                 >  1:824067/1‑93 (MQ=255)
                                         ggTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc                                                 <  2:4910764/93‑1 (MQ=255)
                                           ttttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcca                                               >  1:3749598/1‑93 (MQ=255)
                                           ttttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcca                                               <  1:8685217/93‑1 (MQ=255)
                                           ttttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcca                                               <  1:8774948/93‑1 (MQ=255)
                                           ttttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcca                                               <  1:1794370/93‑1 (MQ=255)
                                            tttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccag                                              <  1:3140248/93‑1 (MQ=255)
                                            tttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccag                                              >  1:4260683/1‑93 (MQ=255)
                                              tATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc                                            >  2:2739471/1‑93 (MQ=255)
                                              tATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc                                            <  1:3643042/93‑1 (MQ=255)
                                                tCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTAATAACGCCATTCGCCAGCCAt                                          <  1:4458001/93‑1 (MQ=255)
                                                 cACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGGGAAGGGATGTTAAGCACTCAGTAAAGGCAGTGGATTGATACCGCCATTCGCCAGCCATg                                         <  2:1714791/93‑1 (MQ=255)
                                                  cccGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCCCGCAGTAAAGGCGGTGGATTGATAACGCCATTCGCCAGCCATGa                                        <  2:9091205/92‑1 (MQ=255)
                                                  cccGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGa                                        <  1:8053892/92‑1 (MQ=255)
                                                  aCCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGa                                        >  2:3910611/1‑93 (MQ=255)
                                                   ccGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGac                                       <  2:9302572/93‑1 (MQ=255)
                                                    cGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGaca                                      <  2:1425656/93‑1 (MQ=255)
                                                    cGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGaca                                      <  1:7877193/93‑1 (MQ=255)
                                                     ggCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGacac                                     <  1:7971340/93‑1 (MQ=255)
                                                     ggCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGacac                                     <  1:6425089/93‑1 (MQ=255)
                                                     ggCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGacac                                     >  2:8202335/1‑93 (MQ=255)
                                                      gCGAAACCTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt                                    <  2:376677/93‑1 (MQ=255)
                                                         aaaCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCg                                 >  1:5598612/1‑93 (MQ=255)
                                                            cGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTa                              <  2:6034220/93‑1 (MQ=255)
                                                            cGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTa                              <  2:3478776/93‑1 (MQ=255)
                                                            cGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTa                              <  1:6662476/93‑1 (MQ=255)
                                                             gTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAt                             <  1:9756166/93‑1 (MQ=255)
                                                             gTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAt                             >  1:1315409/1‑93 (MQ=255)
                                                              gaGCTCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGAATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAtt                            <  1:6786342/92‑1 (MQ=255)
                                                                 ctccTCTTCCAGGTCATTGAAGGGAGGTTCAGCGCTCAGTAAAGGCATTAGATTGATACCGCCATTCGCCAGCCATGACACTGCGGTATTaaa                         <  2:6327017/91‑1 (MQ=255)
                                                                   ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       <  2:5578677/93‑1 (MQ=255)
                                                                   ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       >  1:1902765/1‑93 (MQ=255)
                                                                   ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTAAGTAAAGGCAGTGGATTGATACCGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       <  1:2725469/93‑1 (MQ=255)
                                                                   ccTCTTCCAGGTCAGGGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       >  1:5375943/1‑93 (MQ=255)
                                                                    ctctTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTc                      >  1:8383412/1‑93 (MQ=255)
                                                                          cAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGt                <  1:1088825/93‑1 (MQ=255)
                                                                          cAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGt                <  1:5248348/93‑1 (MQ=255)
                                                                           aGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTa               <  2:6769125/93‑1 (MQ=255)
                                                                           aGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTa               <  1:4016308/93‑1 (MQ=255)
                                                                            ggTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTAGCTGGTaa              >  1:5475734/1‑93 (MQ=255)
                                                                              tCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTTCGGTATTAAATTCGCTGGTaaaa            >  2:6910521/1‑93 (MQ=255)
                                                                              tCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaaa            <  2:4550053/93‑1 (MQ=255)
                                                                              tCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaaa            >  1:5964091/1‑93 (MQ=255)
                                                                               cAGGGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAc           >  2:2348794/1‑93 (MQ=255)
                                                                                 gTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACga         >  2:8693195/1‑92 (MQ=255)
                                                                                  tGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGa        >  2:7383721/1‑93 (MQ=255)
                                                                                   gAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGaa       <  1:5680822/93‑1 (MQ=255)
                                                                                    aaGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGaaa      <  2:5761101/93‑1 (MQ=255)
                                                                                      gggATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAaga    >  1:4988962/1‑93 (MQ=255)
                                                                                       ggATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAagag   >  2:5451022/1‑93 (MQ=255)
                                                                                       ggATGTTAAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAagag   <  2:8574694/93‑1 (MQ=255)
                                                                                        gATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGc  <  1:9293004/93‑1 (MQ=255)
                                                                                        gATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGc  <  1:2475655/93‑1 (MQ=255)
                                                                                        gATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGc  <  1:213952/93‑1 (MQ=255)
                                                                                           |                                                                                         
TTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC  >  NC_000913/4490932‑4491112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 260 total aligned reads displayed.
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  NC_000913/4490930‑4491116
                                                                                             |                                                                                             
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATG                                                                                               <  SRR4302540.2232925/94‑1 (MQ=60)
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATG                                                                                               >  SRR4302540.3594246/1‑94 (MQ=60)
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATG                                                                                               <  SRR4302540.2928121/94‑1 (MQ=60)
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATG                                                                                               <  SRR4302540.1204248/94‑1 (MQ=60)
 TTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGT                                                                                              >  SRR4302540.3118769/1‑94 (MQ=60)
 TTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGT                                                                                              <  SRR4302540.686369/94‑1 (MQ=60)
  TTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTT                                                                                             >  SRR4302540.8586700/1‑94 (MQ=60)
  TTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATT                                                                                             <  SRR4302540.3782570/94‑1 (MQ=60)
  TTATTTATCTCTTAGAAAACAAGCT‑GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTC                                                                                            >  SRR4302540.4044596/1‑94 (MQ=60)
   TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTC                                                                                            <  SRR4302540.9337979/94‑1 (MQ=60)
   TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTC                                                                                            >  SRR4302540.3821203/1‑94 (MQ=60)
    TTTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCA                                                                                           <  SRR4302540.6833575/94‑1 (MQ=60)
    ATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCA                                                                                           >  SRR4302540.9522833/1‑94 (MQ=60)
       TATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCA                                                                                        >  SRR4302540.7615471/1‑94 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.4397399/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       >  SRR4302540.5698864/1‑94 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.5618519/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.2367704/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.4656057/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.1143473/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302540.9364326/94‑1 (MQ=60)
        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAACAC                                                                                       >  SRR4302540.8332034/1‑94 (MQ=60)
         TCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACT                                                                                      <  SRR4302540.6479785/94‑1 (MQ=60)
           TCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCA                                                                                    <  SRR4302540.4073878/94‑1 (MQ=60)
           TCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCA                                                                                    >  SRR4302540.3703542/1‑94 (MQ=60)
            CTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAG                                                                                   >  SRR4302540.9947693/1‑94 (MQ=60)
              TAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTA                                                                                 <  SRR4302540.9100855/94‑1 (MQ=60)
               AGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAA                                                                                <  SRR4302540.7421331/94‑1 (MQ=60)
               AGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAA                                                                                <  SRR4302540.6321043/94‑1 (MQ=60)
               AGAAAACAAGCTGGACTTTTGCTGCCCGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAA                                                                                >  SRR4302540.7639137/1‑94 (MQ=60)
                GAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCCTTAAA                                                                               >  SRR4302540.3486996/1‑94 (MQ=60)
                GAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAA                                                                               <  SRR4302540.1992707/94‑1 (MQ=60)
                GAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAA                                                                               <  SRR4302540.2725871/94‑1 (MQ=60)
                 AAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAG                                                                              <  SRR4302540.7126124/94‑1 (MQ=60)
                 AAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAG                                                                              <  SRR4302540.5729169/94‑1 (MQ=60)
                 AAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAG                                                                              >  SRR4302540.4609103/1‑94 (MQ=60)
                   AACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGC                                                                            >  SRR4302540.9303249/1‑94 (MQ=60)
                   AACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGC                                                                            >  SRR4302540.1246088/1‑94 (MQ=60)
                   AACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGA                                                                            >  SRR4302540.763981/1‑94 (MQ=60)
                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           <  SRR4302540.9003473/94‑1 (MQ=60)
                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           <  SRR4302540.9003474/94‑1 (MQ=60)
                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           >  SRR4302540.984672/1‑94 (MQ=60)
                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           <  SRR4302540.9917795/94‑1 (MQ=60)
                     CAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAG                                                                          >  SRR4302540.5190922/1‑94 (MQ=60)
                     CAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAG                                                                          <  SRR4302540.8128625/94‑1 (MQ=60)
                     CAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAG                                                                          <  SRR4302540.10118687/94‑1 (MQ=60)
                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         >  SRR4302540.513401/1‑94 (MQ=60)
                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         <  SRR4302540.4084940/94‑1 (MQ=60)
                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         <  SRR4302540.9734099/94‑1 (MQ=60)
                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGG                                                                         >  SRR4302540.7494152/1‑94 (MQ=60)
                       AGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTG                                                                        >  SRR4302540.10122373/1‑94 (MQ=60)
                        GCTGGACTTTTGATGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGG                                                                       >  SRR4302540.548310/1‑94 (MQ=60)
                        ACTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGAGG                                                                       <  SRR4302540.8631078/94‑1 (MQ=60)
                         CTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGA                                                                      >  SRR4302540.1234836/1‑94 (MQ=60)
                          TGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAT                                                                     >  SRR4302540.5851281/1‑94 (MQ=60)
                          TGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAT                                                                     >  SRR4302540.6849892/1‑94 (MQ=60)
                          TGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAT                                                                     >  SRR4302540.2968402/1‑94 (MQ=60)
                          TGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGAT                                                                     <  SRR4302540.7920278/94‑1 (MQ=60)
                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCCCTCAGTAAAGGCAGTGGATTG                                                                   <  SRR4302540.6327827/94‑1 (MQ=60)
                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTG                                                                   <  SRR4302540.8935099/94‑1 (MQ=60)
                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTG                                                                   <  SRR4302540.6327825/94‑1 (MQ=60)
                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTG                                                                   <  SRR4302540.9898706/94‑1 (MQ=60)
                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTG                                                                   <  SRR4302540.2812201/94‑1 (MQ=60)
                              CTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAT                                                                 >  SRR4302540.4400628/1‑94 (MQ=60)
                              CTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAT                                                                 >  SRR4302540.8830935/1‑94 (MQ=60)
                              CTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAT                                                                 >  SRR4302540.8830936/1‑94 (MQ=60)
                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                <  SRR4302540.3210415/94‑1 (MQ=60)
                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                <  SRR4302540.8235878/94‑1 (MQ=60)
                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                <  SRR4302540.3892632/94‑1 (MQ=60)
                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                <  SRR4302540.956900/94‑1 (MQ=60)
                                TTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAA                                                               <  SRR4302540.3948255/94‑1 (MQ=60)
                                TTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAA                                                               <  SRR4302540.8372283/94‑1 (MQ=60)
                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302540.8582523/94‑1 (MQ=60)
                                  TGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACG                                                             <  SRR4302540.652721/94‑1 (MQ=60)
                                    CTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCC                                                           <  SRR4302540.2291170/94‑1 (MQ=60)
                                     TGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAANGGCAGTGGATTGANAACGCCA                                                          >  SRR4302540.6935200/1‑94 (MQ=60)
                                     TGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCA                                                          >  SRR4302540.5344164/1‑94 (MQ=60)
                                     TGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCA                                                          <  SRR4302540.2231174/94‑1 (MQ=60)
                                       CCTGGGTTTTATCACCGGCGAAACGTAGCTCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATT                                                        <  SRR4302540.4874076/94‑1 (MQ=60)
                                       CCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATT                                                        <  SRR4302540.4232279/94‑1 (MQ=60)
                                       CCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATT                                                        <  SRR4302540.4592988/94‑1 (MQ=60)
                                       CCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTGCCAGGGCAGTGAAGGGATGTTCAGCCCTCAGTAAAGGCAGTGGATTGATAACGCCATT                                                        <  SRR4302540.4874077/94‑1 (MQ=60)
                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       <  SRR4302540.8056266/94‑1 (MQ=60)
                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       >  SRR4302540.7147088/1‑94 (MQ=60)
                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      <  SRR4302540.227832/94‑1 (MQ=60)
                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      <  SRR4302540.1117785/94‑1 (MQ=60)
                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      <  SRR4302540.876884/94‑1 (MQ=60)
                                          GGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGC                                                     >  SRR4302540.832830/1‑94 (MQ=60)
                                          GGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGC                                                     >  SRR4302540.2947603/1‑94 (MQ=60)
                                           GGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCC                                                    <  SRR4302540.1343059/94‑1 (MQ=60)
                                            GTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCA                                                   <  SRR4302540.4351492/94‑1 (MQ=60)
                                            GTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCA                                                   >  SRR4302540.3790508/1‑94 (MQ=60)
                                             ttttcgcacccggGAAACCTAGCGCCTCTTCCAGGTCAGTTAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  <  SRR4302540.2662545/81‑1 (MQ=60)
                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  <  SRR4302540.1813881/94‑1 (MQ=60)
                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  <  SRR4302540.6311680/94‑1 (MQ=60)
                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  >  SRR4302540.4307028/1‑94 (MQ=60)
                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  <  SRR4302540.8786070/94‑1 (MQ=60)
                                              TTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGC                                                 <  SRR4302540.3174493/94‑1 (MQ=60)
                                                TATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCA                                               <  SRR4302540.3682942/94‑1 (MQ=60)
                                                 ATCACCGGGGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCCCTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAT                                              <  SRR4302540.4776849/94‑1 (MQ=60)
                                                 ATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAT                                              >  SRR4302540.4841266/1‑94 (MQ=60)
                                                  TCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATG                                             <  SRR4302540.1455540/94‑1 (MQ=60)
                                                    CCCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGAC                                           <  SRR4302540.8147423/94‑1 (MQ=60)
                                                    ACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGAC                                           >  SRR4302540.6614291/1‑94 (MQ=60)
                                                     CCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACA                                          <  SRR4302540.2087766/94‑1 (MQ=60)
                                                     CCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACA                                          <  SRR4302540.6462893/94‑1 (MQ=60)
                                                      CGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACAC                                         <  SRR4302540.4832284/94‑1 (MQ=60)
                                                      CGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACAC                                         <  SRR4302540.5292701/94‑1 (MQ=60)
                                                      CGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACAC                                         <  SRR4302540.7370212/94‑1 (MQ=60)
                                                      CGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACAC                                         <  SRR4302540.7969196/94‑1 (MQ=60)
                                                       GGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACT                                        <  SRR4302540.8064224/94‑1 (MQ=60)
                                                       CGCGAAGCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCCCTCAGTAAAGGAAGTGGATTGATAACGCCTTTCCCCAGCCATGACACT                                        <  SRR4302540.2368583/94‑1 (MQ=60)
                                                        GCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTG                                       <  SRR4302540.5345416/94‑1 (MQ=60)
                                                         CGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGC                                      <  SRR4302540.454176/94‑1 (MQ=60)
                                                         CGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGC                                      <  SRR4302540.5931329/94‑1 (MQ=60)
                                                          GAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCG                                     >  SRR4302540.4217279/1‑94 (MQ=60)
                                                            AACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGC                                   >  SRR4302540.425627/1‑94 (MQ=60)
                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 <  SRR4302540.1311324/94‑1 (MQ=60)
                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 >  SRR4302540.1329087/1‑94 (MQ=60)
                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 >  SRR4302540.9574116/1‑94 (MQ=60)
                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 <  SRR4302540.8785611/94‑1 (MQ=60)
                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 <  SRR4302540.6794807/94‑1 (MQ=60)
                                                               GTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATT                                <  SRR4302540.9870623/94‑1 (MQ=60)
                                                               GTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATT                                >  SRR4302540.9016899/1‑94 (MQ=60)
                                                                TAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTA                               <  SRR4302540.3204887/94‑1 (MQ=60)
                                                                TAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTA                               <  SRR4302540.6861381/94‑1 (MQ=60)
                                                                TAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGAGGTATTA                               >  SRR4302540.5541157/1‑94 (MQ=60)
                                                                 AGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAA                              <  SRR4302540.9366908/94‑1 (MQ=60)
                                                                  GCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAA                             <  SRR4302540.1650436/94‑1 (MQ=60)
                                                                   cccccctcCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATACCCCCATTCGCCAGCCATGACACTGCGGTATTAAAT                            <  SRR4302540.1770890/86‑1 (MQ=60)
                                                                   CGCCTCTTCCAGTTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACCCCATTCGCCAGCCATGACACTGCGGTATTAAAT                            <  SRR4302540.5119586/94‑1 (MQ=60)
                                                                   CGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAT                            <  SRR4302540.8084739/94‑1 (MQ=60)
                                                                    GCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302540.1923571/1‑94 (MQ=60)
                                                                    GCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302540.3957648/1‑94 (MQ=60)
                                                                    GCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302540.1923570/1‑94 (MQ=60)
                                                                    GCCTCTTCCAGGTCAGGGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302540.5435057/1‑94 (MQ=60)
                                                                    CCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           <  SRR4302540.8826456/94‑1 (MQ=60)
                                                                     CCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTC                          >  SRR4302540.639167/1‑94 (MQ=60)
                                                                     CCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTC                          >  SRR4302540.8480682/1‑94 (MQ=60)
                                                                     CCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTAAGTAAAGGCAGTGGATTGATACCGCCATTCGCCAGCCATGACACTGCGGTATTAAATTC                          <  SRR4302540.2754862/94‑1 (MQ=60)
                                                                      CTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTAGCCAGCCATGACACTGCGGTATTAAATTCG                         <  SRR4302540.1817778/94‑1 (MQ=60)
                                                                      CTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTAAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCG                         <  SRR4302540.806501/94‑1 (MQ=60)
                                                                       TCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGC                        >  SRR4302540.615547/1‑94 (MQ=60)
                                                                        CTTCCGGGTCAGTGAAGGGATGTTCAGCACTCATTAAAGCCAGTGAATTGATAACCCCATCCGCCAGCCATGACACTGCGGTATTAAATCCGCT                       <  SRR4302540.8949005/94‑1 (MQ=60)
                                                                        CTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCT                       <  SRR4302540.1014043/94‑1 (MQ=60)
                                                                         TTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTG                      >  SRR4302540.5229252/1‑94 (MQ=60)
                                                                           CCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGT                    <  SRR4302540.1351094/94‑1 (MQ=60)
                                                                           CCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGT                    >  SRR4302540.3100496/1‑94 (MQ=60)
                                                                           CCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGA                    >  SRR4302540.3000170/1‑94 (MQ=60)
                                                                           CCAGCTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCTTTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGT                    <  SRR4302540.1243791/94‑1 (MQ=60)
                                                                            CAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTA                   >  SRR4302540.1343666/1‑94 (MQ=60)
                                                                            CAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTA                   <  SRR4302540.5305594/94‑1 (MQ=60)
                                                                            CAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTA                   <  SRR4302540.1099998/94‑1 (MQ=60)
                                                                             AGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAA                  <  SRR4302540.4059568/94‑1 (MQ=60)
                                                                             AGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTAGCTGGTAA                  >  SRR4302540.5536003/1‑94 (MQ=60)
                                                                              GGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAA                 <  SRR4302540.8025978/94‑1 (MQ=60)
                                                                              GGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAA                 <  SRR4302540.6507939/94‑1 (MQ=60)
                                                                              GGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAA                 <  SRR4302540.8376125/94‑1 (MQ=60)
                                                                               GTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAA                >  SRR4302540.4331137/1‑94 (MQ=60)
                                                                               GTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAA                <  SRR4302540.6103858/94‑1 (MQ=60)
                                                                               GTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAA                <  SRR4302540.7925937/94‑1 (MQ=60)
                                                                                TCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAC               <  SRR4302540.841303/94‑1 (MQ=60)
                                                                                TCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAC               <  SRR4302540.1595220/94‑1 (MQ=60)
                                                                                TCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAC               <  SRR4302540.2531594/94‑1 (MQ=60)
                                                                                 CAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACG              >  SRR4302540.3412472/1‑94 (MQ=60)
                                                                                 CAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACG              <  SRR4302540.5172972/94‑1 (MQ=60)
                                                                                  AGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAGATTCGCTGGTAAAACGG             <  SRR4302540.1204977/94‑1 (MQ=60)
                                                                                   GTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGA            >  SRR4302540.3613510/1‑94 (MQ=60)
                                                                                   GTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGA            >  SRR4302540.5658764/1‑94 (MQ=60)
                                                                                   GTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGA            >  SRR4302540.4907290/1‑94 (MQ=60)
                                                                                    TGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAA           >  SRR4302540.8762238/1‑94 (MQ=60)
                                                                                     GAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAA          <  SRR4302540.5743364/94‑1 (MQ=60)
                                                                                     GAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAA          >  SRR4302540.6171615/1‑94 (MQ=60)
                                                                                      AAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         >  SRR4302540.2374527/1‑94 (MQ=60)
                                                                                      AAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         >  SRR4302540.9701041/1‑94 (MQ=60)
                                                                                      AAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         >  SRR4302540.3770445/1‑94 (MQ=60)
                                                                                       AGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        <  SRR4302540.5684005/94‑1 (MQ=60)
                                                                                       AGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        >  SRR4302540.5042976/1‑94 (MQ=60)
                                                                                       AGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        <  SRR4302540.6927086/94‑1 (MQ=60)
                                                                                       AGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        <  SRR4302540.5418262/94‑1 (MQ=60)
                                                                                        GGGATGTTCAGCACTCAGTAAAGGCATGGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302540.5184201/94‑1 (MQ=60)
                                                                                        GGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302540.8738148/94‑1 (MQ=60)
                                                                                        GGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302540.8737869/94‑1 (MQ=60)
                                                                                        GGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302540.1916980/94‑1 (MQ=60)
                                                                                        GGGATATTCAGCAGTCAGTAAAGGCAGTGGATTGTTAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302540.8738149/94‑1 (MQ=60)
                                                                                         GGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC      >  SRR4302540.739886/1‑94 (MQ=60)
                                                                                         GGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC      >  SRR4302540.5799423/1‑94 (MQ=60)
                                                                                         GGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC      <  SRR4302540.424067/94‑1 (MQ=60)
                                                                                         GGATGTCCAGCACTCAGTAAAGGAAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC      <  SRR4302540.5510705/94‑1 (MQ=60)
                                                                                         GGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC      >  SRR4302540.8915446/1‑94 (MQ=60)
                                                                                          GATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCC     <  SRR4302540.2502310/94‑1 (MQ=60)
                                                                                          GATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCC     >  SRR4302540.1593473/1‑94 (MQ=60)
                                                                                          GATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCC     <  SRR4302540.9404044/94‑1 (MQ=60)
                                                                                          GATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCC     <  SRR4302540.216044/94‑1 (MQ=60)
                                                                                           ATGTTCAGCACTCAGTAAAGGCAGTGGATTGATTACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCT    <  SRR4302540.9827485/94‑1 (MQ=60)
                                                                                           ATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCT    <  SRR4302540.5063479/94‑1 (MQ=60)
                                                                                           ATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCT    <  SRR4302540.7149059/94‑1 (MQ=60)
                                                                                            TGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGGCTC   >  SRR4302540.10160697/1‑94 (MQ=60)
                                                                                             GTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  <  SRR4302540.2552190/94‑1 (MQ=60)
                                                                                             GTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  SRR4302540.3244208/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  NC_000913/4490930‑4491116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑