Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,491,023	A→G	Y318H (TAT→CAT)	idnD ←	L‑idonate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,491,023	0	A	G	100.0%	757.8 / NA	235	Y318H (TAT→CAT)	idnD	L‑idonate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (125/110); total (125/110)

Only 100 of 245 total aligned reads displayed.

TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC  >  NC_000913/4490933‑4491112

tatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGtt                                                                                         <  1:5118477/93‑1 (MQ=255)

 atttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTc                                                                                        >  1:250497/1‑93 (MQ=255)

  tttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCa                                                                                       <  2:2339620/93‑1 (MQ=255)

  tttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCa                                                                                       <  2:483619/93‑1 (MQ=255)

   ttatCTCTTAGAAAACAAGCTGGACTTTTACTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAg                                                                                      <  2:2422310/93‑1 (MQ=255)

     atCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCa                                                                                    <  1:5490337/93‑1 (MQ=255)

     atCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCa                                                                                    >  1:2486574/1‑93 (MQ=255)

       ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACt                                                                                  >  1:730343/1‑93 (MQ=255)

       ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACt                                                                                  >  1:901671/1‑93 (MQ=255)

        tctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTc                                                                                 <  1:4857362/93‑1 (MQ=255)

         ctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCa                                                                                >  1:1635162/1‑93 (MQ=255)

         ctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCa                                                                                <  1:2593198/93‑1 (MQ=255)

         ctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCa                                                                                >  2:4270025/1‑93 (MQ=255)

          ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAg                                                                               >  2:1683297/1‑93 (MQ=255)

          ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAg                                                                               >  1:3222478/1‑93 (MQ=255)

           tAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGt                                                                              <  2:1139802/93‑1 (MQ=255)

           tAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGt                                                                              <  2:2350635/93‑1 (MQ=255)

            aGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTa                                                                             <  1:1094901/93‑1 (MQ=255)

            aGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTa                                                                             <  2:6085559/93‑1 (MQ=255)

              aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaaa                                                                           >  2:1339719/1‑93 (MQ=255)

              aaaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTaaa                                                                           >  1:1550685/1‑93 (MQ=255)

               aaaCAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAg                                                                          >  2:4721272/1‑93 (MQ=255)

                  cAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCa                                                                       >  1:3623236/1‑93 (MQ=255)

                  cAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGCAAAGGCa                                                                       >  2:3875212/1‑93 (MQ=255)

                   aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      >  1:4969489/1‑93 (MQ=255)

                   aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      <  1:578396/93‑1 (MQ=255)

                   aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      <  2:1411774/93‑1 (MQ=255)

                   aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      <  2:2838110/93‑1 (MQ=255)

                   aaGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAg                                                                      <  1:4363114/93‑1 (MQ=255)

                    aGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGt                                                                     >  2:5849280/1‑93 (MQ=255)

                     gCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTg                                                                    >  2:603936/1‑93 (MQ=255)

                     gCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTg                                                                    <  2:4837573/93‑1 (MQ=255)

                     gCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTg                                                                    <  2:4514920/93‑1 (MQ=255)

                      cTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTgg                                                                   <  1:5829417/93‑1 (MQ=255)

                      cTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTgg                                                                   >  2:4764650/1‑93 (MQ=255)

                        ggACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAt                                                                 <  1:3449384/93‑1 (MQ=255)

                        ggACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAt                                                                 >  2:4059140/1‑93 (MQ=255)

                        ggACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAt                                                                 <  1:1003045/93‑1 (MQ=255)

                             tttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATa                                                            <  1:6210792/93‑1 (MQ=255)

                             tttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATa                                                            >  1:2223987/1‑93 (MQ=255)

                              ttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATaa                                                           <  1:4728924/93‑1 (MQ=255)

                              ttGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATaa                                                           <  1:5623776/93‑1 (MQ=255)

                               tgctgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAc                                                          <  2:195431/93‑1 (MQ=255)

                                 ctgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGc                                                        >  1:5370338/1‑93 (MQ=255)

                                  tgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGcc                                                       >  1:4072720/1‑93 (MQ=255)

                                  tgcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGcc                                                       >  1:3790306/1‑93 (MQ=255)

                                   gcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCa                                                      >  1:5330509/1‑93 (MQ=255)

                                   gcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCa                                                      >  1:5930400/1‑93 (MQ=255)

                                   gcCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCa                                                      >  2:4502810/1‑93 (MQ=255)

                                     cTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCAtt                                                    >  2:651851/1‑93 (MQ=255)

                                      tGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc                                                   >  1:621135/1‑93 (MQ=255)

                                      tGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc                                                   >  2:3525090/1‑93 (MQ=255)

                                      tGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc                                                   <  1:4584575/93‑1 (MQ=255)

                                      tGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc                                                   >  1:3958264/1‑93 (MQ=255)

                                       gggTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCg                                                  >  1:42726/1‑93 (MQ=255)

                                            ttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagc                                             <  2:5153727/93‑1 (MQ=255)

                                            ttATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagc                                             >  2:678868/1‑93 (MQ=255)

                                             tATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc                                            <  1:3208136/93‑1 (MQ=255)

                                                cACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATg                                         <  1:836272/93‑1 (MQ=255)

                                                 aCCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGa                                        >  2:499562/1‑93 (MQ=255)

                                                 aCCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGa                                        >  2:37075/1‑93 (MQ=255)

                                                   cGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGaca                                      >  2:2530448/1‑93 (MQ=255)

                                                   cGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGaca                                      >  2:3326230/1‑93 (MQ=255)

                                                   cGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGaca                                      <  1:4913475/93‑1 (MQ=255)

                                                    ggCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGacac                                     <  1:1957246/93‑1 (MQ=255)

                                                     gCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt                                    <  2:763693/93‑1 (MQ=255)

                                                     gCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt                                    <  1:3951564/93‑1 (MQ=255)

                                                       gAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAGGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGc                                  <  2:261105/93‑1 (MQ=255)

                                                        aaaCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCg                                 >  2:2038252/1‑93 (MQ=255)

                                                         aaCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCgg                                >  1:3893623/1‑93 (MQ=255)

                                                         aaCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCgg                                >  1:4177997/1‑93 (MQ=255)

                                                          aCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGt                               <  2:883188/93‑1 (MQ=255)

                                                            gTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAt                             >  1:141316/1‑93 (MQ=255)

                                                             tAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAtt                            <  1:5639361/93‑1 (MQ=255)

                                                             tAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAtt                            >  1:4035005/1‑93 (MQ=255)

                                                               gcgcCTCTTCCAGGTCAGTGATGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTaa                          >  2:3599466/1‑93 (MQ=255)

                                                                 gcCTCTTCAAGGTCAGTGAAGGTAGCTTCAGCACCAAGTAAAGGCAGTGGATTAAAAACGCTATTCGCCAGCCATGACACTGCAGTATTAAAt                        <  1:2382180/93‑1 (MQ=255)

                                                                  ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       <  2:5787959/93‑1 (MQ=255)

                                                                  ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       >  1:6079557/1‑93 (MQ=255)

                                                                  ccTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       >  1:3544798/1‑93 (MQ=255)

                                                                  ccTCTTCCAGGGCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAAtt                       <  2:5694027/93‑1 (MQ=255)

                                                                   ctctTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTc                      <  1:2899428/93‑1 (MQ=255)

                                                                    tctTCCAGGTCAGTGCAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCg                     >  2:3770865/1‑93 (MQ=255)

                                                                    tctTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCg                     >  1:517100/1‑93 (MQ=255)

                                                                       tCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTg                  <  1:114534/93‑1 (MQ=255)

                                                                       tCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTg                  <  2:3372258/93‑1 (MQ=255)

                                                                       tCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTg                  >  2:4365482/1‑93 (MQ=255)

                                                                        ccAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTgg                 >  1:3118134/1‑93 (MQ=255)

                                                                         cAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGt                >  2:1848648/1‑93 (MQ=255)

                                                                         cAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGt                >  1:2706365/1‑93 (MQ=255)

                                                                          aGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTa               >  2:5332833/1‑93 (MQ=255)

                                                                          aGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTa               >  2:3001235/1‑93 (MQ=255)

                                                                            gTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaa             >  2:3986102/1‑93 (MQ=255)

                                                                             tCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaaa            >  2:5214939/1‑93 (MQ=255)

                                                                                  gAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGaa       >  2:5671777/1‑93 (MQ=255)

                                                                                  gAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGaa       <  1:48369/93‑1 (MQ=255)

                                                                                   aaGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGaaa      <  1:2032743/93‑1 (MQ=255)

                                                                                     gggATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAaga    >  2:4621537/1‑93 (MQ=255)

                                                                                      ggATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAagag   <  2:5587223/93‑1 (MQ=255)

                                                                                       gATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGc  >  2:3175743/1‑93 (MQ=255)

TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGC  >  NC_000913/4490933‑4491112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  NC_000913/4490930‑4491116

CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATG                                                                                               <  SRR4302533.5142356/94‑1 (MQ=60)

   TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTC                                                                                            >  SRR4302533.252042/1‑94 (MQ=60)

   TATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTC                                                                                            <  SRR4302533.5149403/94‑1 (MQ=60)

    ATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCA                                                                                           >  SRR4302533.5194979/1‑94 (MQ=60)

    ATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCA                                                                                           >  SRR4302533.5024427/1‑94 (MQ=60)

    ATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAAGGATGTTCA                                                                                           >  SRR4302533.387850/1‑94 (MQ=60)

     TTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAG                                                                                          <  SRR4302533.3949198/94‑1 (MQ=60)

       TATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCA                                                                                        >  SRR4302533.2501893/1‑94 (MQ=60)

        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302533.4070179/94‑1 (MQ=60)

        ATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCAC                                                                                       <  SRR4302533.5523434/94‑1 (MQ=60)

         TCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACT                                                                                      <  SRR4302533.2364131/94‑1 (MQ=60)

         TCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACT                                                                                      >  SRR4302533.734648/1‑94 (MQ=60)

         TCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACT                                                                                      <  SRR4302533.3776932/94‑1 (MQ=60)

         TCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACT                                                                                      >  SRR4302533.906960/1‑94 (MQ=60)

          CTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTC                                                                                     >  SRR4302533.4122824/1‑94 (MQ=60)

           TCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCA                                                                                    <  SRR4302533.4886801/94‑1 (MQ=60)

           TCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCA                                                                                    >  SRR4302533.1645066/1‑94 (MQ=60)

            CTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAG                                                                                   >  SRR4302533.3242351/1‑94 (MQ=60)

            CTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAG                                                                                   <  SRR4302533.2609132/94‑1 (MQ=60)

            CTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAG                                                                                   >  SRR4302533.4319724/1‑94 (MQ=60)

            CTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAG                                                                                   <  SRR4302533.1022071/94‑1 (MQ=60)

             TTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGT                                                                                  >  SRR4302533.33738/1‑94 (MQ=60)

             TTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGT                                                                                  >  SRR4302533.3096854/1‑94 (MQ=60)

             TTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGT                                                                                  >  SRR4302533.494698/1‑94 (MQ=60)

              TAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTA                                                                                 <  SRR4302533.932319/94‑1 (MQ=60)

              TAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTA                                                                                 >  SRR4302533.4895042/1‑94 (MQ=60)

               ATAAAACAAGCTGGACTTTTGCTTCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAA                                                                                >  SRR4302533.62666/1‑94 (MQ=60)

               AGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAA                                                                                <  SRR4302533.1101381/94‑1 (MQ=60)

                GAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAA                                                                               >  SRR4302533.1560027/1‑94 (MQ=60)

                 AAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAG                                                                              <  SRR4302533.1273043/94‑1 (MQ=60)

                  AAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGG                                                                             <  SRR4302533.6235323/94‑1 (MQ=60)

                   AACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGC                                                                            >  SRR4302533.4037221/1‑94 (MQ=60)

                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           >  SRR4302533.3645532/1‑94 (MQ=60)

                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           <  SRR4302533.4755076/94‑1 (MQ=60)

                    ACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCA                                                                           >  SRR4302533.537477/1‑94 (MQ=60)

                     CAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAG                                                                          >  SRR4302533.2554749/1‑94 (MQ=60)

                     CAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAG                                                                          >  SRR4302533.4999618/1‑94 (MQ=60)

                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         >  SRR4302533.4205394/1‑94 (MQ=60)

                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         <  SRR4302533.4389452/94‑1 (MQ=60)

                      AAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGT                                                                         <  SRR4302533.581806/94‑1 (MQ=60)

                        GCTGGTCTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGG                                                                       <  SRR4302533.487805/94‑1 (MQ=60)

                        GCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGG                                                                       <  SRR4302533.131203/94‑1 (MQ=60)

                         CTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGA                                                                      <  SRR4302533.5864644/94‑1 (MQ=60)

                          TGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGAT                                                                     <  SRR4302533.4856167/94‑1 (MQ=60)

                           GGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATT                                                                    <  SRR4302533.1008908/94‑1 (MQ=60)

                           GGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATT                                                                    <  SRR4302533.3470682/94‑1 (MQ=60)

                            GACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTACCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTG                                                                   >  SRR4302533.2421540/1‑94 (MQ=60)

                              CTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAT                                                                 >  SRR4302533.3537136/1‑94 (MQ=60)

                              CTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGAT                                                                 >  SRR4302533.6000935/1‑94 (MQ=60)

                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                >  SRR4302533.2237721/1‑94 (MQ=60)

                               TTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATA                                                                <  SRR4302533.1642023/94‑1 (MQ=60)

                                TTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAA                                                               <  SRR4302533.5608521/94‑1 (MQ=60)

                                TTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAA                                                               <  SRR4302533.6248040/94‑1 (MQ=60)

                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCTCTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302533.5005801/94‑1 (MQ=60)

                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302533.6197436/94‑1 (MQ=60)

                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302533.5657768/94‑1 (MQ=60)

                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302533.4757593/94‑1 (MQ=60)

                                 TTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAAC                                                              <  SRR4302533.3227766/94‑1 (MQ=60)

                                   GCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGC                                                            >  SRR4302533.5402606/1‑94 (MQ=60)

                                    CTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCC                                                           >  SRR4302533.4097514/1‑94 (MQ=60)

                                    CTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCC                                                           >  SRR4302533.3813514/1‑94 (MQ=60)

                                     TGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCA                                                          >  SRR4302533.5966252/1‑94 (MQ=60)

                                     TGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCA                                                          >  SRR4302533.5362457/1‑94 (MQ=60)

                                       CCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATT                                                        >  SRR4302533.4932474/1‑94 (MQ=60)

                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       >  SRR4302533.3982388/1‑94 (MQ=60)

                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       >  SRR4302533.624785/1‑94 (MQ=60)

                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       >  SRR4302533.624784/1‑94 (MQ=60)

                                        CTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTC                                                       >  SRR4302533.4495447/1‑94 (MQ=60)

                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      >  SRR4302533.2088498/1‑94 (MQ=60)

                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      <  SRR4302533.4612346/94‑1 (MQ=60)

                                         TGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCG                                                      >  SRR4302533.43016/1‑94 (MQ=60)

                                          GGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGC                                                     >  SRR4302533.5914967/1‑94 (MQ=60)

                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  <  SRR4302533.3979758/94‑1 (MQ=60)

                                             TTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAG                                                  >  SRR4302533.4757989/1‑94 (MQ=60)

                                                TATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCA                                               <  SRR4302533.3227893/94‑1 (MQ=60)

                                                 ATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAT                                              >  SRR4302533.5663765/1‑94 (MQ=60)

                                                 ATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAT                                              <  SRR4302533.435146/94‑1 (MQ=60)

                                                  TCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATG                                             <  SRR4302533.987555/94‑1 (MQ=60)

                                                   CACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGA                                            >  SRR4302533.5913058/1‑94 (MQ=60)

                                                   CACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGA                                            <  SRR4302533.841171/94‑1 (MQ=60)

                                                    ACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGAC                                           <  SRR4302533.855707/94‑1 (MQ=60)

                                                    AACGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGAC                                           <  SRR4302533.699670/94‑1 (MQ=60)

                                                      CGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACAC                                         <  SRR4302533.4943236/94‑1 (MQ=60)

                                                       GGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACT                                        <  SRR4302533.1969191/94‑1 (MQ=60)

                                                       GGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACT                                        <  SRR4302533.5455101/94‑1 (MQ=60)

                                                        GCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTG                                       <  SRR4302533.5709403/94‑1 (MQ=60)

                                                        GCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTG                                       <  SRR4302533.3975653/94‑1 (MQ=60)

                                                         CGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGC                                      >  SRR4302533.177593/1‑94 (MQ=60)

                                                           AAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGG                                    >  SRR4302533.3917383/1‑94 (MQ=60)

                                                           AAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGG                                    >  SRR4302533.4203309/1‑94 (MQ=60)

                                                             ACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTA                                  <  SRR4302533.5829363/94‑1 (MQ=60)

                                                             ACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTA                                  <  SRR4302533.4192297/94‑1 (MQ=60)

                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGCTAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 >  SRR4302533.3629047/1‑94 (MQ=60)

                                                              CGTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTAT                                 >  SRR4302533.142219/1‑94 (MQ=60)

                                                               GTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATT                                <  SRR4302533.2801985/94‑1 (MQ=60)

                                                               GTAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATT                                >  SRR4302533.4059567/1‑94 (MQ=60)

                                                                TAGCGCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTA                               <  SRR4302533.5673452/94‑1 (MQ=60)

                                                                    GCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302533.6116325/1‑94 (MQ=60)

                                                                    GCCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATT                           >  SRR4302533.3566646/1‑94 (MQ=60)

                                                                    GCCTCTTCAAGGTCAGTGAAGGTAGCTTCAGCACCAAGTAAAGGCAGTGGATTAAAAACGCTATTCGCCAGCCATGACACTGCAGTATTAAATT                           <  SRR4302533.2396911/94‑1 (MQ=42)

                                                                     CCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTC                          <  SRR4302533.2704703/94‑1 (MQ=60)

                                                                     CCTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTC                          <  SRR4302533.2506721/94‑1 (MQ=60)

                                                                      CTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCG                         >  SRR4302533.520170/1‑94 (MQ=60)

                                                                      CTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCG                         <  SRR4302533.2917246/94‑1 (MQ=60)

                                                                      CTCTTCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGACAGCCATGACACTGCGGTATTAAATTCG                         >  SRR4302533.3935808/1‑94 (MQ=60)

                                                                         TTCCAGGTCAGTGAAGGTATGTTCAGCACTCAGTAAAGGCATTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTG                      <  SRR4302533.3161853/94‑1 (MQ=60)

                                                                          TCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGG                     >  SRR4302533.3137307/1‑94 (MQ=60)

                                                                          TCCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGG                     <  SRR4302533.115261/94‑1 (MQ=60)

                                                                           CCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGT                    >  SRR4302533.2722966/1‑94 (MQ=60)

                                                                           CCAGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGT                    <  SRR4302533.4004924/94‑1 (MQ=60)

                                                                             AGGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAA                  <  SRR4302533.1131718/94‑1 (MQ=60)

                                                                              GGTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAA                 <  SRR4302533.3232428/94‑1 (MQ=60)

                                                                               GTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAA                <  SRR4302533.198082/94‑1 (MQ=60)

                                                                               GTCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAA                <  SRR4302533.2544573/94‑1 (MQ=60)

                                                                                TCAGTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAC               <  SRR4302533.5812973/94‑1 (MQ=60)

                                                                                TCAGTAAAGGGATGTTCAGCACTAAGTAAAGTCAGTGGATTAATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAAC               <  SRR4302533.75288/94‑1 (MQ=60)

                                                                                   GTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTACAACGGA            >  SRR4302533.1479077/1‑94 (MQ=60)

                                                                                   GTGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGA            >  SRR4302533.3456409/1‑94 (MQ=60)

                                                                                    TGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAA           <  SRR4302533.5313061/94‑1 (MQ=60)

                                                                                    TGAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAA           <  SRR4302533.3575707/94‑1 (MQ=60)

                                                                                     GAAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAA          <  SRR4302533.48703/94‑1 (MQ=60)

                                                                                      AGGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         <  SRR4302533.1640512/94‑1 (MQ=60)

                                                                                      AAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         <  SRR4302533.5339561/94‑1 (MQ=60)

                                                                                      AAGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAG         <  SRR4302533.2045284/94‑1 (MQ=60)

                                                                                       AGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        >  SRR4302533.5462680/1‑94 (MQ=60)

                                                                                       AGGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGA        >  SRR4302533.2755145/1‑94 (MQ=60)

                                                                                        GGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAG       <  SRR4302533.2369213/94‑1 (MQ=60)

                                                                                        GGGATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGCAAAGAG       >  SRR4302533.2010783/1‑94 (MQ=60)

                                                                                           ATGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCT    >  SRR4302533.2004311/1‑94 (MQ=60)

                                                                                            TGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTC   >  SRR4302533.6021220/1‑94 (MQ=60)

                                                                                            TGTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTC   >  SRR4302533.4689566/1‑94 (MQ=60)

                                                                                             TTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  <  SRR4302533.2606483/94‑1 (MQ=60)

                                                                                             GTTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  SRR4302533.312129/1‑94 (MQ=60)

CTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCT  >  NC_000913/4490930‑4491116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`