Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,588,681	A→G	Y62H (TAC→CAC)	yjiY ←	putative carbon starvation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,588,681	0	A	G	99.7%	1083.4 / ‑5.5	330	Y62H (TAC→CAC)	yjiY	putative carbon starvation protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (198/131); total (198/132)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 341 total aligned reads displayed.

GGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATG  >  NC_000913/4588591‑4588770

 gTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAg                                                                                        >  1:1539791/1‑93 (MQ=255)

 gTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAg                                                                                        >  1:7090147/1‑93 (MQ=255)

  ttGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGa                                                                                       >  1:2032886/1‑93 (MQ=255)

  gtGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGa                                                                                       <  1:3972825/92‑1 (MQ=255)

   tGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAc                                                                                      <  1:849722/93‑1 (MQ=255)

   tGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAc                                                                                      <  1:2809710/93‑1 (MQ=255)

   tGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAc                                                                                      >  1:2386912/1‑93 (MQ=255)

    ggTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACt                                                                                     <  2:7552810/93‑1 (MQ=255)

       cGGAGCGTAGTTGAGACCGCTGTTGTTAATAAGCTCACGCGTCGGGCGCGGTGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTgta                                                                                  <  1:6988662/93‑1 (MQ=255)

        ggAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTgtag                                                                                 >  1:7807453/1‑93 (MQ=255)

         gAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTgtagt                                                                                <  1:7999780/93‑1 (MQ=255)

          aaCGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTgtagta                                                                               >  1:9472835/1‑93 (MQ=255)

            cGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGc                                                                             >  2:8343954/1‑93 (MQ=255)

            cGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTAATCACCTTCTGGGCGGTGTGCAGACTggagtatg                                                                             >  1:4396596/1‑91 (MQ=255)

              tAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGa                                                                           <  1:2516021/93‑1 (MQ=255)

              tAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGa                                                                           >  1:7517064/1‑93 (MQ=255)

               aGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGAt                                                                          >  2:6050159/1‑93 (MQ=255)

               aGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGAt                                                                          <  2:2415488/93‑1 (MQ=255)

                gTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATa                                                                         >  2:349223/1‑93 (MQ=255)

                gTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATa                                                                         <  1:1040463/93‑1 (MQ=255)

                 ttCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAc                                                                        >  1:3423485/1‑93 (MQ=255)

                 ttCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAc                                                                        >  1:1669480/1‑93 (MQ=255)

                    aGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGcc                                                                     <  2:428778/93‑1 (MQ=255)

                     gACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGcca                                                                    >  1:5104275/1‑93 (MQ=255)

                       ccGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGccacc                                                                  <  2:8676454/93‑1 (MQ=255)

                        cgtcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGccacca                                                                 >  1:9954320/1‑93 (MQ=255)

                          tcgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGa                                                               >  1:494294/1‑93 (MQ=255)

                           cgtTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGAt                                                              >  2:1085414/1‑93 (MQ=255)

                            gttgttAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGAtc                                                             >  1:7530566/1‑92 (MQ=255)

                             ttgttAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATac                                                            >  1:4838263/1‑93 (MQ=255)

                                ttAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACAcc                                                         <  2:8615285/93‑1 (MQ=255)

                                ttAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACAcc                                                         <  2:942408/93‑1 (MQ=255)

                                 taataaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCg                                                        >  1:5283239/1‑93 (MQ=255)

                                 taataaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCg                                                        >  1:207980/1‑93 (MQ=255)

                                   ataaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGAt                                                      <  1:8625627/93‑1 (MQ=255)

                                   ataaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGAt                                                      >  2:4855571/1‑93 (MQ=255)

                                    taaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATa                                                     >  1:5948183/1‑93 (MQ=255)

                                    taaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATa                                                     >  1:3468193/1‑93 (MQ=255)

                                     aaCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATAc                                                    >  1:3216835/1‑93 (MQ=255)

                                      aCCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACa                                                   >  2:2035706/1‑93 (MQ=255)

                                        cGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACaga                                                 <  1:4113673/93‑1 (MQ=255)

                                        cGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACaga                                                 <  1:3727467/93‑1 (MQ=255)

                                        cGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACaga                                                 <  1:7927986/93‑1 (MQ=255)

                                        cGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACaga                                                 <  1:8142150/93‑1 (MQ=255)

                                        cGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCCTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACAGATACaga                                                 >  2:9583465/1‑93 (MQ=255)

                                         gCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACagag                                                <  1:9067709/93‑1 (MQ=255)

                                         gCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACagag                                                <  2:3724733/93‑1 (MQ=255)

                                          cAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAgg                                               <  2:6829545/93‑1 (MQ=255)

                                          cAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAgg                                               >  2:1552021/1‑93 (MQ=255)

                                           aGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGc                                              >  2:5889208/1‑93 (MQ=255)

                                           aGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGc                                              >  2:692100/1‑93 (MQ=255)

                                           aGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGc                                              >  2:4383319/1‑93 (MQ=255)

                                           aGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACAACGATACAGAGGc                                              >  1:6633635/1‑93 (MQ=255)

                                            ggCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCg                                             >  2:4931306/1‑93 (MQ=255)

                                            ggCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCg                                             >  1:4737725/1‑93 (MQ=255)

                                            ggCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCg                                             >  2:8842242/1‑93 (MQ=255)

                                               gTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGAcc                                          >  1:9570585/1‑93 (MQ=255)

                                                tCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCa                                         >  1:5675043/1‑93 (MQ=255)

                                                 cgcgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCAc                                        >  1:9769662/1‑93 (MQ=255)

                                                  gcgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACg                                       >  2:7157859/1‑93 (MQ=255)

                                                  gcgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACg                                       >  2:7868024/1‑93 (MQ=255)

                                                  gcgcgcgTGGGGTCGAGTTTCATCACCTTCTCGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACCGAGCCGACCACg                                       >  2:2156978/1‑93 (MQ=255)

                                                   cgcgcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGa                                      >  2:5765694/1‑93 (MQ=255)

                                                     ggcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATc                                    <  1:3812365/92‑1 (MQ=255)

                                                      gcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATcc                                   <  1:885613/93‑1 (MQ=255)

                                                      gcgTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATcc                                   >  1:545468/1‑93 (MQ=255)

                                                       cgTGGGGTCGAGTTTCATCCCCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCca                                  <  1:1174205/93‑1 (MQ=255)

                                                        gTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGAACACGATCcac                                 >  1:2763422/1‑93 (MQ=255)

                                                        gTGGGATCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCcac                                 <  2:7614517/93‑1 (MQ=255)

                                                         tGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGTTACACCGATACAGAGGCGACAACGATCcaca                                >  1:8199589/1‑93 (MQ=255)

                                                         tGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCcaca                                >  1:8705592/1‑93 (MQ=255)

                                                          ggggTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAg                               >  1:4597668/1‑93 (MQ=255)

                                                           gggTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAgg                              <  2:3926745/93‑1 (MQ=255)

                                                           gggTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAgg                              <  1:4269021/93‑1 (MQ=255)

                                                           gggTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAgg                              <  2:1663721/93‑1 (MQ=255)

                                                             gTCGAGTTTCATCACCTTCTGGGCTATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGgc                            <  2:8645691/93‑1 (MQ=255)

                                                             gTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGgc                            >  2:3111878/1‑93 (MQ=255)

                                                              tCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGgcg                           <  2:3237347/93‑1 (MQ=255)

                                                               cGAGTTGTATTACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACTACGATCCACAGGgcgc                          >  2:8991008/1‑93 (MQ=255)

                                                                gAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCt                         >  1:6404693/1‑93 (MQ=255)

                                                                 aGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTg                        >  2:2652939/1‑93 (MQ=255)

                                                                  gTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGa                       >  1:7625970/1‑93 (MQ=255)

                                                                  gTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGa                       <  2:4548469/93‑1 (MQ=255)

                                                                  gTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGa                       <  1:6519407/93‑1 (MQ=255)

                                                                   tttCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAt                      >  1:1858540/1‑93 (MQ=255)

                                                                   tttCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAt                      >  1:5849933/1‑93 (MQ=255)

                                                                    ttCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAtg                     <  2:8558295/93‑1 (MQ=255)

                                                                    ttCACCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAtg                     <  2:3883927/93‑1 (MQ=255)

                                                                        tcgccTGCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCt                 <  1:1001007/90‑1 (MQ=255)

                                                                        tcaCCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCt                 >  2:6610163/1‑93 (MQ=255)

                                                                         caCCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTc                <  2:7799097/93‑1 (MQ=255)

                                                                          cccTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGCCCACGATCCACAGGGCGCTGATGTGCTcc               <  2:3362948/92‑1 (MQ=255)

                                                                            cTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTcccc             >  1:5559281/1‑93 (MQ=255)

                                                                             ttCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTccccc            >  2:1910655/1‑93 (MQ=255)

                                                                             ttCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTccccc            >  2:1219525/1‑93 (MQ=255)

                                                                                tGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCgc         >  2:10033969/1‑92 (MQ=255)

                                                                                tGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCgc         >  1:7365254/1‑92 (MQ=255)

                                                                                tGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGa         >  2:6769741/1‑93 (MQ=255)

                                                                                  ggCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACg       <  2:811044/93‑1 (MQ=255)

                                                                                     gATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTaa    <  1:9174931/93‑1 (MQ=255)

GGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATG  >  NC_000913/4588591‑4588770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 203 total aligned reads displayed.

AACGGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAAC  >  NC_000913/4588588‑4588774

AACGGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTG                                                                                               >  SRR4302540.4435875/1‑94 (MQ=60)

  CGGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCA                                                                                             >  SRR4302540.3827545/1‑94 (MQ=60)

   GGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAG                                                                                            >  SRR4302540.7169045/1‑94 (MQ=60)

   GGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAG                                                                                            >  SRR4302540.1556813/1‑94 (MQ=60)

   GGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAG                                                                                            >  SRR4302540.1472259/1‑94 (MQ=60)

   GGTTGGTCGGAACGTAGTTCAGAACGTCGTTGTTAATAACCGCAGGAGTCGCGCGCGTGGGGTCGAGTTTCATCACATTCTGGGCGATGTGCAG                                                                                            >  SRR4302540.900681/1‑94 (MQ=60)

    GTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGA                                                                                           >  SRR4302540.2054816/1‑94 (MQ=60)

    GCTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGA                                                                                           <  SRR4302540.7960623/94‑1 (MQ=60)

     TTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAC                                                                                          >  SRR4302540.2412639/1‑94 (MQ=60)

     TTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAC                                                                                          >  SRR4302540.1744516/1‑94 (MQ=60)

     GTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGAC                                                                                          <  SRR4302540.4015646/94‑1 (MQ=60)

      TGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACT                                                                                         <  SRR4302540.338396/94‑1 (MQ=60)

      TGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACT                                                                                         <  SRR4302540.248543/94‑1 (MQ=60)

      TGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACT                                                                                         <  SRR4302540.5478958/94‑1 (MQ=60)

      TGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACT                                                                                         <  SRR4302540.858739/94‑1 (MQ=60)

      TGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACT                                                                                         <  SRR4302540.2840052/94‑1 (MQ=60)

          CGGAGCGTAGTTGAGACCGCTGTTGTTAATAAGCTCACGCGTCGGGCGCGGTGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAG                                                                                     <  SRR4302540.7066247/94‑1 (MQ=32)

          CGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAG                                                                                     >  SRR4302540.7898689/1‑94 (MQ=60)

          CGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAG                                                                                     >  SRR4302540.322660/1‑94 (MQ=60)

          CGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAG                                                                                     <  SRR4302540.7066248/94‑1 (MQ=60)

           GGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGT                                                                                    >  SRR4302540.5177348/1‑94 (MQ=60)

           GGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGCTTCATCACCTTCTGGGCGATGTGCAGACTGTAGT                                                                                    >  SRR4302540.6099205/1‑94 (MQ=60)

            GAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTA                                                                                   <  SRR4302540.8092893/94‑1 (MQ=60)

            GAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTA                                                                                   >  SRR4302540.5536977/1‑94 (MQ=60)

            GAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTA                                                                                   >  SRR4302540.9585195/1‑94 (MQ=60)

              ACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTAATCACCTTCTGGGCGGTGTGCAGACTGgagtatg                                                                                 >  SRR4302540.4444294/1‑87 (MQ=60)

                GTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGA                                                                               >  SRR4302540.7601112/1‑94 (MQ=60)

                GTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGA                                                                               >  SRR4302540.8656608/1‑94 (MQ=60)

                GTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGA                                                                               >  SRR4302540.2819786/1‑94 (MQ=60)

                GTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGAGA                                                                               >  SRR4302540.6861709/1‑94 (MQ=60)

                 TAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGAT                                                                              <  SRR4302540.9051895/94‑1 (MQ=60)

                 TAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGAT                                                                              <  SRR4302540.2543200/94‑1 (MQ=60)

                  AGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATA                                                                             <  SRR4302540.7128537/94‑1 (MQ=60)

                  AGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATA                                                                             >  SRR4302540.9815764/1‑94 (MQ=60)

                  AGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCCCGCGT‑GGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            <  SRR4302540.10022931/94‑1 (MQ=60)

                   GTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            >  SRR4302540.5196347/1‑94 (MQ=60)

                   GTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            <  SRR4302540.7009324/94‑1 (MQ=60)

                   GTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            >  SRR4302540.1687645/1‑94 (MQ=60)

                   GTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            <  SRR4302540.1051043/94‑1 (MQ=60)

                   GTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            >  SRR4302540.3460707/1‑94 (MQ=60)

                   GTTCAGACCGTCGTTGTGAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATAC                                                                            <  SRR4302540.7009069/94‑1 (MQ=60)

                       AGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCA                                                                        >  SRR4302540.5159530/1‑94 (MQ=60)

                       AGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCA                                                                        >  SRR4302540.3732567/1‑94 (MQ=60)

                        GACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCAC                                                                       >  SRR4302540.2098832/1‑94 (MQ=60)

                        GACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCAC                                                                       >  SRR4302540.7674427/1‑94 (MQ=60)

                        GACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCAC                                                                       >  SRR4302540.7674429/1‑94 (MQ=60)

                         ACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACC                                                                      <  SRR4302540.886659/94‑1 (MQ=60)

                          CCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCA                                                                     >  SRR4302540.10070513/1‑94 (MQ=60)

                          CCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGCCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCA                                                                     >  SRR4302540.1060095/1‑94 (MQ=60)

                           CGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAG                                                                    <  SRR4302540.2918131/94‑1 (MQ=60)

                           CGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAG                                                                    <  SRR4302540.802885/94‑1 (MQ=60)

                            GTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGA                                                                   >  SRR4302540.6198004/1‑94 (MQ=60)

                            GTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGA                                                                   >  SRR4302540.499595/1‑94 (MQ=60)

                            CTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGA                                                                   <  SRR4302540.4033563/94‑1 (MQ=60)

                             tgtTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGC‑ATGTGCAGACTGTAGTAGCGATACGCCACCAGATA                                                                 <  SRR4302540.7270376/91‑1 (MQ=60)

                             TCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGAT                                                                  >  SRR4302540.8259511/1‑94 (MQ=60)

                             TCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGAT                                                                  <  SRR4302540.2812941/94‑1 (MQ=60)

                              CGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATC                                                                 >  SRR4302540.7614871/1‑94 (MQ=60)

                              CGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATA                                                                 >  SRR4302540.8790446/1‑94 (MQ=60)

                              CGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATA                                                                 >  SRR4302540.2414510/1‑94 (MQ=60)

                              CGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATA                                                                 >  SRR4302540.468090/1‑94 (MQ=60)

                               GTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATAC                                                                >  SRR4302540.4890088/1‑94 (MQ=60)

                               GTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATAC                                                                >  SRR4302540.4890087/1‑94 (MQ=60)

                               GTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATAC                                                                >  SRR4302540.9719082/1‑94 (MQ=60)

                               GTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATAC                                                                <  SRR4302540.6008668/94‑1 (MQ=60)

                               GTTGTTAATAACCGCAGGCGGCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATAC                                                                <  SRR4302540.9615883/94‑1 (MQ=60)

                                TTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACA                                                               <  SRR4302540.5773476/94‑1 (MQ=60)

                                TTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACA                                                               >  SRR4302540.1051258/1‑94 (MQ=60)

                                TTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACA                                                               <  SRR4302540.3279189/94‑1 (MQ=60)

                                 TGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACAC                                                              >  SRR4302540.5943797/1‑94 (MQ=60)

                                   TTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCG                                                            >  SRR4302540.5340967/1‑94 (MQ=60)

                                   TTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCG                                                            >  SRR4302540.8828471/1‑94 (MQ=60)

                                   TTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCG                                                            >  SRR4302540.210018/1‑94 (MQ=60)

                                   TTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCG                                                            >  SRR4302540.210019/1‑94 (MQ=60)

                                   TTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCG                                                            >  SRR4302540.8052642/1‑94 (MQ=60)

                                    TAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGA                                                           >  SRR4302540.6559248/1‑94 (MQ=60)

                                     AATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGAT                                                          >  SRR4302540.4027248/1‑94 (MQ=60)

                                     AATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACATTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGAT                                                          >  SRR4302540.186331/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTANCGNNACGCCACCAGANACACCGNTA                                                         >  SRR4302540.877407/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATA                                                         >  SRR4302540.9617710/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATA                                                         >  SRR4302540.3795592/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATA                                                         >  SRR4302540.3505949/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATA                                                         >  SRR4302540.6013953/1‑94 (MQ=60)

                                      ATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATA                                                         <  SRR4302540.8725826/94‑1 (MQ=60)

                                       TAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATAC                                                        <  SRR4302540.3480038/94‑1 (MQ=60)

                                       TAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATAC                                                        >  SRR4302540.3251773/1‑94 (MQ=60)

                                        AACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACA                                                       >  SRR4302540.7408512/1‑94 (MQ=60)

                                        AACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACA                                                       >  SRR4302540.4349645/1‑94 (MQ=60)

                                        AACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGAGCGATGTGCAGACTGTAGTAACGATACGCCACCAGATACACCGATACA                                                       >  SRR4302540.5690181/1‑94 (MQ=60)

                                          CCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGA                                                     <  SRR4302540.7582392/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.6666753/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.3768133/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.8020386/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.8236652/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.4158089/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    <  SRR4302540.8684302/94‑1 (MQ=60)

                                           CGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAG                                                    >  SRR4302540.352802/1‑94 (MQ=60)

                                            GCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGCGG                                                   >  SRR4302540.1787352/1‑94 (MQ=60)

                                            GCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGG                                                   <  SRR4302540.9176588/94‑1 (MQ=60)

                                             CAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACAACGATACAGAGGC                                                  >  SRR4302540.6707052/1‑94 (MQ=60)

                                              AGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCG                                                 >  SRR4302540.7614249/1‑94 (MQ=60)

                                              AGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCG                                                 <  SRR4302540.1589514/94‑1 (MQ=60)

                                              AGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCG                                                 >  SRR4302540.4788296/1‑94 (MQ=60)

                                              AGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCG                                                 >  SRR4302540.10133672/1‑94 (MQ=60)

                                              AGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACAGATACAGAGGCG                                                 >  SRR4302540.7331357/1‑94 (MQ=60)

                                               GGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGA                                                >  SRR4302540.6671670/1‑94 (MQ=60)

                                                GCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGAC                                               <  SRR4302540.8484812/94‑1 (MQ=60)

                                                 CGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACC                                              >  SRR4302540.9683653/1‑94 (MQ=60)

                                                  GTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCA                                             >  SRR4302540.9449124/1‑94 (MQ=60)

                                                  GTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCA                                             >  SRR4302540.76847/1‑94 (MQ=60)

                                                  GTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCA                                             >  SRR4302540.5737532/1‑94 (MQ=60)

                                                   TCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCAC                                            >  SRR4302540.9884214/1‑94 (MQ=60)

                                                   TCGCGCGCGGGGGGTCGAGTTTCATCCCCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCAC                                            <  SRR4302540.8111131/94‑1 (MQ=60)

                                                    CGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACG                                           <  SRR4302540.4720241/94‑1 (MQ=60)

                                                    CGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACG                                           <  SRR4302540.4826098/94‑1 (MQ=60)

                                                    CGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACG                                           <  SRR4302540.4826099/94‑1 (MQ=60)

                                                     GCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGA                                          <  SRR4302540.8099754/94‑1 (MQ=60)

                                                      CGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGAT                                         <  SRR4302540.9269265/94‑1 (MQ=60)

                                                      CGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGAT                                         <  SRR4302540.7799393/94‑1 (MQ=60)

                                                      CGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGAT                                         <  SRR4302540.1886198/94‑1 (MQ=60)

                                                       GCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATC                                        <  SRR4302540.4640100/94‑1 (MQ=60)

                                                       GCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATC                                        <  SRR4302540.2613458/94‑1 (MQ=60)

                                                        GGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCC                                       <  SRR4302540.3853880/94‑1 (MQ=60)

                                                        CGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCC                                       >  SRR4302540.551405/1‑94 (MQ=60)

                                                        CGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGAGACCACGATCC                                       >  SRR4302540.2270771/1‑94 (MQ=60)

                                                         GCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCA                                      <  SRR4302540.895011/94‑1 (MQ=60)

                                                          CGTGGGGTCGAGTTTCATCCCCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCAC                                     <  SRR4302540.1186345/94‑1 (MQ=60)

                                                          CGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCAC                                     >  SRR4302540.1985212/1‑94 (MQ=60)

                                                          CGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCAC                                     >  SRR4302540.5768616/1‑94 (MQ=60)

                                                          CGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGAACACGATCCAC                                     >  SRR4302540.2793277/1‑94 (MQ=60)

                                                          CGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAACGATAAGCCACCAGATACACCGATACAGAGGGGACCACGATCCAC                                     >  SRR4302540.2793276/1‑94 (MQ=60)

                                                           GTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGTTACACCGATACAGAGGCGACAACGATCCACA                                    >  SRR4302540.8294874/1‑94 (MQ=60)

                                                           GTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACA                                    >  SRR4302540.4174669/1‑94 (MQ=60)

                                                           GTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACA                                    >  SRR4302540.8806642/1‑94 (MQ=60)

                                                           GTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACA                                    >  SRR4302540.3500237/1‑94 (MQ=60)

                                                           GTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACA                                    >  SRR4302540.7941901/1‑94 (MQ=60)

                                                            TGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAG                                   <  SRR4302540.10108994/94‑1 (MQ=60)

                                                            TGGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAG                                   >  SRR4302540.4647105/1‑94 (MQ=60)

                                                            GGGGGTCGAGTTTCATCACCTTTTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAG                                   <  SRR4302540.4287701/94‑1 (MQ=60)

                                                             GGGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGG                                  >  SRR4302540.8207829/1‑94 (MQ=60)

                                                              GGGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGG                                 <  SRR4302540.4315455/94‑1 (MQ=60)

                                                               GGTCGAGTTTCATCACCTTCTGGTCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGC                                <  SRR4302540.4390276/94‑1 (MQ=60)

                                                               GGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGC                                <  SRR4302540.4522752/94‑1 (MQ=60)

                                                               GGTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGC                                >  SRR4302540.1016984/1‑94 (MQ=60)

                                                                GTCGAGTTTCATCACCTTCTGGGCGATGTGCAGCCTGTAGTAagcgctagatcggaagagcgggtcagcaggaatgccgagaccgctctcgtag                               >  SRR4302540.7529745/1‑42 (MQ=60)

                                                                GTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCG                               <  SRR4302540.9505248/94‑1 (MQ=60)

                                                                GTCGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCG                               <  SRR4302540.7926515/94‑1 (MQ=60)

                                                                  CGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCT                             <  SRR4302540.1205395/94‑1 (MQ=60)

                                                                  CGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCT                             >  SRR4302540.9750686/1‑94 (MQ=60)

                                                                  CGAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCT                             >  SRR4302540.6475986/1‑94 (MQ=60)

                                                                   GAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTG                            <  SRR4302540.760117/94‑1 (MQ=60)

                                                                   GAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTG                            >  SRR4302540.10082988/1‑94 (MQ=60)

                                                                   GAGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTA                            >  SRR4302540.5589669/1‑94 (MQ=60)

                                                                    AGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGA                           >  SRR4302540.7711732/1‑94 (MQ=60)

                                                                    AGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGA                           >  SRR4302540.3681293/1‑94 (MQ=60)

                                                                    AGTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGA                           >  SRR4302540.1652729/1‑94 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGCCTGTAGTAGCGATACGCCACCCGATACACCGACACAGAGGCAACCACGATCCcaagggcgatgag                          >  SRR4302540.2353814/1‑81 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          >  SRR4302540.5914631/1‑94 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          >  SRR4302540.492197/1‑94 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          <  SRR4302540.515298/94‑1 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          >  SRR4302540.1878920/1‑94 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          <  SRR4302540.6591823/94‑1 (MQ=60)

                                                                     GTTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGAT                          <  SRR4302540.6591824/94‑1 (MQ=60)

                                                                      TTTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATG                         >  SRR4302540.5084778/1‑94 (MQ=60)

                                                                       TTCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGT                        >  SRR4302540.2261573/1‑94 (MQ=60)

                                                                        TCATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTG                       >  SRR4302540.5356429/1‑94 (MQ=60)

                                                                         CATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGC                      >  SRR4302540.5656086/1‑94 (MQ=60)

                                                                          ATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCT                     <  SRR4302540.2251831/94‑1 (MQ=60)

                                                                          ATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCT                     >  SRR4302540.8323387/1‑94 (MQ=60)

                                                                          ATCACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCT                     <  SRR4302540.539202/94‑1 (MQ=60)

                                                                           TCGCCTGCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTC                    <  SRR4302540.1011175/94‑1 (MQ=60)

                                                                            CACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCC                   >  SRR4302540.8841287/1‑94 (MQ=60)

                                                                            CACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCC                   <  SRR4302540.8648805/94‑1 (MQ=60)

                                                                             ACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATCCGCCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCC                  <  SRR4302540.9495824/94‑1 (MQ=60)

                                                                             ACCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCC                  >  SRR4302540.2565107/1‑94 (MQ=60)

                                                                              CCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCC                 >  SRR4302540.5620506/1‑94 (MQ=60)

                                                                              CCTTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCC                 >  SRR4302540.3860994/1‑94 (MQ=60)

                                                                               CTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCC                <  SRR4302540.6091055/94‑1 (MQ=60)

                                                                                TTCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCC               <  SRR4302540.7786056/94‑1 (MQ=60)

                                                                                 TCTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCG              <  SRR4302540.5019827/94‑1 (MQ=60)

                                                                                  CTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGC             >  SRR4302540.7447372/1‑94 (MQ=60)

                                                                                  CTGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGACCTCTGATGTGCTCACCCCGA             >  SRR4302540.3891795/1‑94 (MQ=60)

                                                                                   TGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGAC            >  SRR4302540.826065/1‑94 (MQ=60)

                                                                                   TGGGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGAC            >  SRR4302540.3404843/1‑94 (MQ=60)

                                                                                     GGCGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGT          >  SRR4302540.9197220/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        >  SRR4302540.5823122/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        >  SRR4302540.9389879/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        >  SRR4302540.4381482/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        >  SRR4302540.4725823/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        >  SRR4302540.136378/1‑94 (MQ=60)

                                                                                       CGATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAA        <  SRR4302540.1839933/94‑1 (MQ=60)

                                                                                        GATGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAAT       <  SRR4302540.9284982/94‑1 (MQ=60)

                                                                                        GATGGGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAAT       <  SRR4302540.8844474/94‑1 (MQ=60)

                                                                                          TGTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGC     >  SRR4302540.6073614/1‑94 (MQ=60)

                                                                                           GTGCAGACTGTAGTGGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCA    <  SRR4302540.8572165/94‑1 (MQ=60)

                                                                                           GTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCA    >  SRR4302540.4713530/1‑94 (MQ=60)

                                                                                           GTGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCA    <  SRR4302540.3827460/94‑1 (MQ=60)

                                                                                            TGCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAC   >  SRR4302540.5424802/1‑94 (MQ=60)

                                                                                             GCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAAC  >  SRR4302540.6875603/1‑94 (MQ=60)

                                                                                             GCAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAAC  <  SRR4302540.9860512/94‑1 (MQ=60)

AACGGTTGGTCGGAACGTAGTTCAGACCGTCGTTGTTAATAACCGCAGGCGTCGCGCGCGTGGGGTCGAGTTTCATCACCTTCTGGGCGATGTACAGACTGTAGTAGCGATACGCCACCAGATACACCGATACAGAGGCGACCACGATCCACAGGGCGCTGATGTGCTCCCCCCGACGTAATGCAAC  >  NC_000913/4588588‑4588774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`