Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,603,785 A→G noncoding (71/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,603,7850AG96.8% 822.1 / 18.5 285noncoding (71/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (158/117);  minor base . (6/3);  total (164/121)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 319 total aligned reads displayed.

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874
                                                                                               |                                                                                         
  ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTg                                                                                       >  1:8316124/1‑93 (MQ=255)
   cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGa                                                                                      <  2:3266873/93‑1 (MQ=255)
    tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGGCTCGTCTGGCGC‑GA‑‑‑GG‑GGGGGGGCTTGaa                                                                                     >  2:7520504/1‑93 (MQ=37)
      aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAAcc                                                                                   <  1:867394/93‑1 (MQ=255)
      aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAAcc                                                                                   <  1:9386136/93‑1 (MQ=255)
       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAAccc                                                                                  <  2:5854613/93‑1 (MQ=255)
          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGGACCCCCa                                                                               >  2:513152/1‑93 (MQ=255)
          tAGCGCCTCTACCAATTCCGCCACCTTCGCAAAGTCATCTTCCTTTTTTTGATATCGCCACGTTTGGGGC‑GAG‑‑GG‑GGGGGGGCTTGAAccccc                                                                                <  2:3085158/93‑1 (MQ=25)
          tAGCCCTTCTACCAATTCCGCCTTCTTCGCTCAGTTATCTTACTTTTTTTGATATCGCCTCGTTGGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAAccccc                                                                                <  2:10037984/93‑1 (MQ=255)
           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCa                                                                               <  2:2080343/93‑1 (MQ=38)
           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCa                                                                               <  1:7781177/93‑1 (MQ=38)
            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCAc                                                                              <  2:1544245/93‑1 (MQ=255)
            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACg                                                                             >  1:6507477/1‑93 (MQ=255)
              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GG‑GGGGGGGCTTGAACCCCCAcc                                                                             >  1:7426915/1‑92 (MQ=38)
              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTc                                                                           >  1:6288005/1‑93 (MQ=255)
               cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTc                                                                           >  1:5955160/1‑93 (MQ=255)
               cgTCTACCAATCCCGCCCCCTTCGCACAGTCATCTTATTTTTTTTGATATCCCCTCTTTTGGTGC‑GAGG‑GG‑GGGGGGGCTTGAACCCCCACGt                                                                            <  2:596696/93‑1 (MQ=25)
                   tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTa                                                                       >  2:7020763/1‑93 (MQ=255)
                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTaa                                                                      >  2:8274189/1‑93 (MQ=255)
                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTaa                                                                      >  2:22210/1‑93 (MQ=255)
                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAg                                                                     <  1:6018160/93‑1 (MQ=255)
                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAg                                                                     >  1:4802323/1‑93 (MQ=255)
                     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGa                                                                    >  1:9091618/1‑93 (MQ=255)
                      cAACTCCCCCCCCTTCCCACAGTCATTTTATTTTTTTTGATTTCGCCTCGTTTGGGGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGa                                                                    <  2:7914546/93‑1 (MQ=21)
                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTTC‑GA‑‑‑GGAGGGGGGGGTTGAACCCCGAGGTCCGAAAAAac                                                                  >  1:8056189/1‑93 (MQ=255)
                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 >  2:2593291/1‑93 (MQ=255)
                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 <  1:5704581/93‑1 (MQ=255)
                          tCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAacac                                                                <  1:9254447/93‑1 (MQ=37)
                          tCCGCCCCCCTCGCACAGGCATTTTTCTTTTTTTGATATCCCCTCGTTTGGGGCGGG‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAacac                                                                <  1:1177257/93‑1 (MQ=21)
                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAaca                                                           >  1:704566/1‑93 (MQ=255)
                             cccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTa                                                              <  1:1141803/92‑1 (MQ=37)
                              ccACCTTCGCACAGTCATCTTCCTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                            <  1:9165113/93‑1 (MQ=37)
                               cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          <  1:2329099/93‑1 (MQ=255)
                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACACCt                                                        >  2:7225319/1‑93 (MQ=255)
                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:4513795/1‑93 (MQ=255)
                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  2:6405509/1‑93 (MQ=255)
                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  2:4536469/1‑93 (MQ=255)
                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACATg                                                       >  2:5244745/1‑93 (MQ=255)
                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGGCTTGAACCCCCCCCGCCGAAAaaacacaaacac                                                          >  2:6600216/1‑93 (MQ=255)
                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  1:9487792/1‑93 (MQ=255)
                                    tCGCACAGTCATCTTACTTTTATTGACATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGTGCCTGAACTCCCACGTCCGTAAGAACATCTACACCTGaa                                                     >  2:8958301/1‑93 (MQ=21)
                                     cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                    <  2:8019170/93‑1 (MQ=255)
                                      gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                    <  1:3427799/93‑1 (MQ=255)
                                      gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                   <  1:7517645/93‑1 (MQ=255)
                                        acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 >  1:2010554/1‑93 (MQ=255)
                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  2:4289944/1‑93 (MQ=255)
                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGACGCTAgcg                                              >  1:9426424/1‑93 (MQ=255)
                                               tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          >  2:1774370/1‑93 (MQ=255)
                                                cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGG‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:6745143/93‑1 (MQ=21)
                                                cTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCCCGTCCGTAAGAAACCTAACACCTGAAGCTACGGCGTc                                          >  2:9438005/1‑93 (MQ=18)
                                                    cTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     >  1:2803551/1‑93 (MQ=255)
                                                     tttttttGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCGCGTCCGTAAGAACACTAACACCTGAACCTAGCGCGTCTCCCa                                     >  2:5552718/1‑93 (MQ=18)
                                                     tttttttGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     <  1:4367565/93‑1 (MQ=255)
                                                     tttttttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  2:1300390/1‑93 (MQ=255)
                                                     tttgtttgATATCGCCTGGTTGGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    <  2:77640/93‑1 (MQ=17)
                                                      ttttttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                  >  1:3184185/1‑93 (MQ=255)
                                                       tttttGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCTTCTACCAAt                                   >  1:6940954/1‑93 (MQ=17)
                                                         tttGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  1:8123162/93‑1 (MQ=255)
                                                          ttGATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                >  2:5060981/1‑93 (MQ=255)
                                                            gATATCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGc                              >  2:3122357/1‑93 (MQ=17)
                                                             atatCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  1:3027734/1‑93 (MQ=255)
                                                              tatCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGccc                            >  1:3135218/1‑92 (MQ=14)
                                                              tatCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCa                            >  1:4600011/1‑93 (MQ=14)
                                                               ttCGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           <  1:4111930/92‑1 (MQ=14)
                                                                tCGCCTCGTTTGGTGC‑GAGG‑GG‑GGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGAGTCTACCAATTCCCCCAc                           >  2:3492928/1‑93 (MQ=14)
                                                                tCGCCTCGTTTGGTGC‑GAGG‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACAATAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCAc                           >  1:5604897/1‑93 (MQ=14)
                                                                tCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:5745905/1‑93 (MQ=255)
                                                                tCGCCTCGTTTGGGGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                          <  2:4771833/93‑1 (MQ=14)
                                                                 cGCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         >  1:9730170/1‑93 (MQ=255)
                                                                  gCCTCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  1:99313/1‑93 (MQ=255)
                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       <  1:5328783/93‑1 (MQ=255)
                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       <  1:6214847/93‑1 (MQ=255)
                                                                     tCGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                     >  1:1457410/1‑93 (MQ=255)
                                                                      cGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                    >  2:8756650/1‑93 (MQ=255)
                                                                      cGTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                    >  1:3758272/1‑93 (MQ=255)
                                                                       gTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                   >  2:3812217/1‑93 (MQ=255)
                                                                       gTTTGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                   >  1:7605792/1‑93 (MQ=255)
                                                                       gTTTGGTGC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 >  2:986092/1‑93 (MQ=255)
                                                                        tttGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  2:1573278/93‑1 (MQ=255)
                                                                        tgtGGTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  1:287590/91‑1 (MQ=11)
                                                                          tGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg               <  1:5045531/93‑1 (MQ=255)
                                                                          tGGTGC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg               <  1:2488989/93‑1 (MQ=255)
                                                                            gTGC‑GAG‑‑GG‑GGGGGGGCTTGAACCCCCACGCCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc              >  2:2910434/1‑93 (MQ=14)
                                                                              gC‑GA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg           <  1:8564670/93‑1 (MQ=255)
                                                                              gC‑GA‑‑‑GG‑GGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc          <  1:5770547/93‑1 (MQ=255)
                                                                                 gA‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTTCCAATTCCGCCACCTTCGCCCACTGCggaca         >  2:2322516/1‑89 (MQ=14)
                                                                                  gagggg‑ggggggCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg           <  2:5261617/89‑1 (MQ=255)
                                                                                  a‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                              >  1:3565123/1‑55 (MQ=255)
                                                                                  a‑‑‑GG‑GGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  1:6672232/93‑1 (MQ=255)
                                                                                     ggg‑ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  1:4645063/92‑1 (MQ=255)
                                                                                     ggg‑ggggggGCTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  1:1865332/92‑1 (MQ=255)
                                                                                      gg‑ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              >  2:6852779/1‑70 (MQ=25)
                                                                                      gg‑ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat       >  2:610000/1‑93 (MQ=255)
                                                                                       g‑gggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAata      <  2:8834508/93‑1 (MQ=255)
                                                                                       g‑gggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata      <  2:2825511/93‑1 (MQ=255)
                                                                                         ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat     >  1:3863490/1‑93 (MQ=255)
                                                                                         ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat     <  2:4901219/93‑1 (MQ=255)
                                                                                           ggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa   >  2:1683632/1‑93 (MQ=255)
                                                                                           ggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa   <  2:6744535/93‑1 (MQ=255)
                                                                                           ggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa   <  1:9850838/93‑1 (MQ=255)
                                                                                               |                                                                                         
CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603692‑4603878
                                                                                                  |                                                                                             
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGG                                                                                               >  SRR4302540.4677147/1‑94 (MQ=60)
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGG                                                                                               >  SRR4302540.232706/1‑94 (MQ=60)
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGG                                                                                               >  SRR4302540.2496964/1‑94 (MQ=60)
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGG                                                                                               <  SRR4302540.3580038/94‑1 (MQ=60)
  ACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCT                                                                                             >  SRR4302540.3792482/1‑94 (MQ=60)
   CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTT                                                                                            >  SRR4302540.7568820/1‑94 (MQ=60)
    CCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTG                                                                                           <  SRR4302540.3886661/94‑1 (MQ=60)
    ACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTG                                                                                           >  SRR4302540.8412658/1‑94 (MQ=60)
        GAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAAC                                                                                        <  SRR4302540.7148784/94‑1 (MQ=60)
         AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCC                                                                                      <  SRR4302540.9497855/94‑1 (MQ=60)
         AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCC                                                                                      <  SRR4302540.876595/94‑1 (MQ=60)
           GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCC                                                                                    <  SRR4302540.9444211/94‑1 (MQ=60)
            CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCC                                                                                    >  SRR4302540.4185274/1‑94 (MQ=60)
             GAGCGCGTCTACCAATTCCGCCACCTCCGCACATTCATCTTACTTTTTTTGATATCCCCTCGTTTGG‑TGCGGG‑‑‑GGGGGGGGGCTTGAACCCCCA                                                                                   <  SRR4302540.9823484/94‑1 (MQ=60)
              AGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCAC                                                                                  <  SRR4302540.7872131/94‑1 (MQ=60)
              AGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACG                                                                                 >  SRR4302540.6579794/1‑94 (MQ=60)
              AGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACG                                                                                 >  SRR4302540.6579795/1‑94 (MQ=60)
               GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACG                                                                                 <  SRR4302540.5315193/94‑1 (MQ=60)
               GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACG                                                                                 >  SRR4302540.250537/1‑94 (MQ=60)
                CGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACC                                                                                 >  SRR4302540.7509652/1‑94 (MQ=60)
                CGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTC                                                                               >  SRR4302540.6357997/1‑94 (MQ=60)
                 GCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTC                                                                               >  SRR4302540.6021020/1‑94 (MQ=60)
                 GCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302540.292579/1‑94 (MQ=60)
                  CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302540.378237/1‑94 (MQ=60)
                  CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302540.10021457/1‑94 (MQ=60)
                    TCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGT                                                                            >  SRR4302540.5047843/1‑94 (MQ=60)
                    TCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTA                                                                           >  SRR4302540.128421/1‑94 (MQ=60)
                      TACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAG                                                                         >  SRR4302540.4853718/1‑94 (MQ=60)
                       ACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGA                                                                        >  SRR4302540.9200846/1‑94 (MQ=60)
                       ACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGA                                                                        <  SRR4302540.6084910/94‑1 (MQ=60)
                          AATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                      >  SRR4302540.9988136/1‑94 (MQ=60)
                          AATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     >  SRR4302540.7820553/1‑94 (MQ=60)
                          AATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGATTGAACCCCCACGTCCGCAAGCACA                                                                     >  SRR4302540.9854674/1‑94 (MQ=60)
                          AATTCCACCACCTTCGCACAGTCATTTTACTTTTTTTGATATGTCCTCGTTGGG‑GGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                      <  SRR4302540.9745177/94‑1 (MQ=57)
                           ATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCCCGTCCCTCAGAAC                                                                      >  SRR4302540.3952221/1‑94 (MQ=60)
                           ATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.5767385/94‑1 (MQ=60)
                           AATCCGCCCCCTTCGCACAGTCATCTTTCTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     <  SRR4302540.9956930/94‑1 (MQ=60)
                            tcccccccccttcccacagtcttcttctTTTTTTTGATCTCCCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.42919/66‑1 (MQ=41)
                            TTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.949476/94‑1 (MQ=60)
                            TTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.6987166/94‑1 (MQ=60)
                            TTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.4480161/94‑1 (MQ=60)
                             TCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACT                                                                   <  SRR4302540.9365201/94‑1 (MQ=60)
                             TCCGCCCCCCTCGCACAGGCATTTTTCTTTTTTTGATATCCCCTCGTTTGG‑GGCGGG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACT                                                                   <  SRR4302540.1189423/94‑1 (MQ=23)
                             TCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACT                                                                   <  SRR4302540.8631106/94‑1 (MQ=60)
                              CCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTA                                                                  >  SRR4302540.7300761/1‑94 (MQ=60)
                               ctccccccccttgccgacatcgtccttcgttttgtcaattcctcgccgtttggggcgagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     <  SRR4302540.9219965/35‑1 (MQ=59)
                               atcccccccccttccaaaagtatttttatttttttggattccccccgttttggggggggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     <  SRR4302540.5477411/35‑1 (MQ=59)
                               CGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               >  SRR4302540.712130/1‑94 (MQ=60)
                               CGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               <  SRR4302540.2903393/94‑1 (MQ=60)
                                ttccgccacttcctaacactccctttatttttttcaattccgcaggtttgggcgggggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302540.9478607/36‑1 (MQ=60)
                                CCCACCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302540.1371026/94‑1 (MQ=60)
                                CCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                 <  SRR4302540.1153738/94‑1 (MQ=60)
                                 CCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               <  SRR4302540.2624086/94‑1 (MQ=60)
                                 CCACCTTCGCACAGTCATCTTCCTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               <  SRR4302540.9275085/94‑1 (MQ=60)
                                  CACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             <  SRR4302540.3156150/94‑1 (MQ=60)
                                  CACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             <  SRR4302540.2354185/94‑1 (MQ=60)
                                  CACCTTCACACATTCATTTTTCTTTTTTTGATATCGCCTTGTTGGG‑TGC‑GAG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACAC                                                              <  SRR4302540.2081652/94‑1 (MQ=46)
                                   CCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             <  SRR4302540.2406932/94‑1 (MQ=60)
                                   CCCTTCCCACAGTCATCTTTCTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAC                                                              <  SRR4302540.6464838/94‑1 (MQ=60)
                                   ACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             >  SRR4302540.6162425/1‑94 (MQ=60)
                                   ACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302540.1156316/1‑94 (MQ=60)
                                   ACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302540.4562699/1‑94 (MQ=60)
                                    cccctctgcgctcagtcattttttttttttttatcccctcccgtttggggcgggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302540.1335607/40‑1 (MQ=21)
                                    CCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            <  SRR4302540.969428/94‑1 (MQ=60)
                                    CCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTG                                                           >  SRR4302540.9600276/1‑94 (MQ=60)
                                     CTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGGGGGGGGGGGGGTTGAACCCCCACGCCCGTAAGAACAaaaaaccc                                                             >  SRR4302540.683316/1‑86 (MQ=60)
                                     CTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTG                                                           <  SRR4302540.8280390/94‑1 (MQ=60)
                                     CTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTA                                                           >  SRR4302540.8454836/1‑94 (MQ=60)
                                      TTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGA                                                          >  SRR4302540.1181672/1‑94 (MQ=60)
                                       TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302540.3201214/94‑1 (MQ=60)
                                       TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302540.3179800/94‑1 (MQ=60)
                                        CGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302540.4869787/94‑1 (MQ=60)
                                        CGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGC                                                       <  SRR4302540.9426605/94‑1 (MQ=60)
                                         cgacactcattttagTTTTTTTGAAAGCGCGTCGTTTGG‑TGC‑GG‑‑‑GGGGGGGGGCTGGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302540.7601708/79‑1 (MQ=19)
                                         GCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGC                                                       <  SRR4302540.3465052/94‑1 (MQ=60)
                                         GCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302540.2381578/94‑1 (MQ=60)
                                         GCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302540.7601707/94‑1 (MQ=60)
                                          CACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                     >  SRR4302540.2032294/1‑94 (MQ=60)
                                           ACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                                     >  SRR4302540.7183656/1‑94 (MQ=60)
                                           ACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                    <  SRR4302540.6644263/94‑1 (MQ=60)
                                            CAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                    >  SRR4302540.9530916/1‑94 (MQ=60)
                                            CAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                    <  SRR4302540.3473248/94‑1 (MQ=60)
                                             AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  >  SRR4302540.86905/1‑94 (MQ=60)
                                             AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGACGCTAGCG                                                  >  SRR4302540.9538427/1‑94 (MQ=60)
                                              GTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   >  SRR4302540.768011/1‑94 (MQ=60)
                                              GTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGC                                                 >  SRR4302540.6797627/1‑94 (MQ=60)
                                              GTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGC                                                 >  SRR4302540.1909407/1‑94 (MQ=60)
                                                CATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑TA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302540.2843171/1‑94 (MQ=60)
                                                CATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302540.3431121/1‑94 (MQ=60)
                                                 ATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAACAACACTAACACCTGAAGCTAGGGCGT                                               >  SRR4302540.6800784/1‑94 (MQ=60)
                                                 ATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGGC                                              >  SRR4302540.6314061/1‑94 (MQ=60)
                                                  TCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             >  SRR4302540.3348284/1‑94 (MQ=60)
                                                  TCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             <  SRR4302540.5127182/94‑1 (MQ=60)
                                                  TCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             >  SRR4302540.5685885/1‑94 (MQ=60)
                                                  TCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             <  SRR4302540.9324706/94‑1 (MQ=60)
                                                   CTTACTTTTTTTGATATCGCCTCGTTTGG‑TGCGGG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             <  SRR4302540.6819672/94‑1 (MQ=60)
                                                   CTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             >  SRR4302540.8166575/1‑94 (MQ=60)
                                                     TACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                           <  SRR4302540.10104420/94‑1 (MQ=60)
                                                     TACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                           >  SRR4302540.9331007/1‑94 (MQ=60)
                                                      ACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCA                                         >  SRR4302540.2833826/1‑94 (MQ=60)
                                                       ttttattttttttataaccgcttgtttgggggcggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             <  SRR4302540.4372440/59‑1 (MQ=9)
                                                       CTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCA                                         >  SRR4302540.9195946/1‑94 (MQ=59)
                                                        ttcttttttttgataaccccgcttttggggcgggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                            <  SRR4302540.7572832/60‑1 (MQ=9)
                                                        TTTTTTTGATCTCGCCTCGTTTGG‑TGCTGG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCAGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                        <  SRR4302540.6067063/94‑1 (MQ=35)
                                                        TTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                        <  SRR4302540.6067064/94‑1 (MQ=60)
                                                        TTTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                        <  SRR4302540.4415026/94‑1 (MQ=60)
                                                        TTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                      >  SRR4302540.3218782/1‑94 (MQ=60)
                                                         TTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCTTCTACCAAT                                       >  SRR4302540.7018219/1‑94 (MQ=52)
                                                         TTTTTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       >  SRR4302540.7018218/1‑94 (MQ=54)
                                                            TTTGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                    <  SRR4302540.8217444/94‑1 (MQ=52)
                                                            TTTGAAATCGCCTCGTTTGGTTGG‑GG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                    <  SRR4302540.8217443/94‑1 (MQ=16)
                                                            TTGGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                    <  SRR4302540.5446691/94‑1 (MQ=43)
                                                             ttttttatatgccctgttttgggggggggGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       <  SRR4302540.296621/65‑1 (MQ=8)
                                                              TGATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                  >  SRR4302540.4784511/1‑94 (MQ=45)
                                                               GATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC                                 >  SRR4302540.3060991/1‑94 (MQ=42)
                                                                ATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                                >  SRR4302540.5808912/1‑94 (MQ=40)
                                                                ATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCC                                >  SRR4302540.3169417/1‑94 (MQ=38)
                                                                ATATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCA                                >  SRR4302540.4649453/1‑94 (MQ=37)
                                                                 TATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               >  SRR4302540.10081948/1‑94 (MQ=38)
                                                                 TATCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               <  SRR4302540.1596837/94‑1 (MQ=40)
                                                                  TTCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                              <  SRR4302540.4156314/94‑1 (MQ=33)
                                                                  ATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACGAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCC                               >  SRR4302540.5881240/1‑94 (MQ=33)
                                                                  ATCGCCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAATAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCAC                               >  SRR4302540.5666564/1‑94 (MQ=32)
                                                                  ATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302540.3017369/1‑94 (MQ=38)
                                                                  ATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            <  SRR4302540.3078213/94‑1 (MQ=39)
                                                                   TCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302540.9844430/1‑94 (MQ=33)
                                                                   TCGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302540.9844429/1‑94 (MQ=33)
                                                                    CGCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            >  SRR4302540.100364/1‑94 (MQ=30)
                                                                    CGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302540.8294580/1‑94 (MQ=33)
                                                                    CGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           <  SRR4302540.1972810/94‑1 (MQ=35)
                                                                     GCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302540.4486814/1‑94 (MQ=28)
                                                                     GCCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302540.7838310/1‑94 (MQ=28)
                                                                     GCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          >  SRR4302540.9123407/1‑94 (MQ=30)
                                                                     GCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          >  SRR4302540.9559488/1‑94 (MQ=30)
                                                                     GCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302540.6283997/94‑1 (MQ=33)
                                                                     GCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302540.5387158/94‑1 (MQ=33)
                                                                     GCCTCGTTGGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302540.6284277/94‑1 (MQ=22)
                                                                      CCTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302540.9974709/1‑94 (MQ=25)
                                                                      CCTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACCGCCGGAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCC                          >  SRR4302540.2056929/1‑94 (MQ=19)
                                                                       CTCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCGCGTCCGTAAGAACACTAACACCTAAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          >  SRR4302540.1849931/1‑94 (MQ=21)
                                                                       CTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302540.9224519/1‑94 (MQ=21)
                                                                       CTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302540.1473267/1‑94 (MQ=21)
                                                                       CTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302540.7033398/1‑94 (MQ=21)
                                                                       CTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         <  SRR4302540.4251978/94‑1 (MQ=21)
                                                                       CTCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCAC                         >  SRR4302540.4602566/1‑94 (MQ=21)
                                                                        TCGTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302540.9261033/1‑94 (MQ=18)
                                                                        TCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGGACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                        >  SRR4302540.8025399/1‑94 (MQ=20)
                                                                        TCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                        >  SRR4302540.3799270/1‑94 (MQ=21)
                                                                        TCGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                        >  SRR4302540.321287/1‑94 (MQ=21)
                                                                         ccctggttgggtgcgggGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           <  SRR4302540.4191089/77‑1 (MQ=7)
                                                                         CGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302540.4637305/1‑94 (MQ=21)
                                                                         CGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302540.7691260/1‑94 (MQ=21)
                                                                         CGTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302540.3565740/1‑94 (MQ=21)
                                                                         CGTTGGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCA                      <  SRR4302540.298318/94‑1 (MQ=18)
                                                                          GTTTGG‑TGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302540.5556736/1‑94 (MQ=18)
                                                                          GTTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT‑AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.7998437/94‑1 (MQ=22)
                                                                          GTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     >  SRR4302540.4842045/1‑94 (MQ=25)
                                                                          GTTTGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     >  SRR4302540.7543522/1‑94 (MQ=25)
                                                                           TTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.6690758/94‑1 (MQ=21)
                                                                           TTTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.6238202/94‑1 (MQ=21)
                                                                           TGTGG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.290430/94‑1 (MQ=18)
                                                                             TGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGC                  <  SRR4302540.2515797/94‑1 (MQ=30)
                                                                             TGG‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGC                  <  SRR4302540.5100099/94‑1 (MQ=30)
                                                                              GG‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGC                  <  SRR4302540.8685799/94‑1 (MQ=28)
                                                                               tttgggtggagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.4347709/83‑1 (MQ=24)
                                                                               tttggggggagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302540.3234573/83‑1 (MQ=24)
                                                                               G‑TGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG                 >  SRR4302540.956829/1‑94 (MQ=31)
                                                                               G‑TGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGA                <  SRR4302540.6020824/94‑1 (MQ=35)
                                                                                  GC‑TA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGAGCA             <  SRR4302540.5834546/94‑1 (MQ=39)
                                                                                  GC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG               <  SRR4302540.681861/94‑1 (MQ=36)
                                                                                  GC‑GA‑‑‑GGGGGGGGTCTTGATCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGC              <  SRR4302540.3905126/94‑1 (MQ=39)
                                                                                  GC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGC              <  SRR4302540.8664133/94‑1 (MQ=40)
                                                                                  GC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGC              <  SRR4302540.4680743/94‑1 (MQ=40)
                                                                                  GC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCA             <  SRR4302540.5834281/94‑1 (MQ=41)
                                                                                   C‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCA             <  SRR4302540.9445192/94‑1 (MQ=43)
                                                                                     GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAA            >  SRR4302540.3604070/1‑94 (MQ=45)
                                                                                      G‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302540.4694783/94‑1 (MQ=47)
                                                                                      G‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302540.4694784/94‑1 (MQ=47)
                                                                                      G‑‑‑GGGGGGGGGCTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302540.1885779/94‑1 (MQ=45)
                                                                                      A‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302540.6745953/94‑1 (MQ=48)
                                                                                        agGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAA            <  SRR4302540.8568530/92‑1 (MQ=46)
                                                                                          GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATA          <  SRR4302540.4971480/94‑1 (MQ=50)
                                                                                           GGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         <  SRR4302540.5758836/94‑1 (MQ=53)
                                                                                           GGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         >  SRR4302540.3905485/1‑94 (MQ=53)
                                                                                           GGGGGGGGCTTGAACCCCCACGTCAGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         <  SRR4302540.5788416/94‑1 (MQ=51)
                                                                                             GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCA       >  SRR4302540.3965691/1‑94 (MQ=58)
                                                                                              GGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA      <  SRR4302540.9965977/94‑1 (MQ=60)
                                                                                              GGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA      >  SRR4302540.2060495/1‑94 (MQ=60)
                                                                                               GGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAC     <  SRR4302540.6534303/94‑1 (MQ=60)
                                                                                                GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    <  SRR4302540.1247361/94‑1 (MQ=60)
                                                                                                GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    >  SRR4302540.4651632/1‑94 (MQ=60)
                                                                                                GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    >  SRR4302540.5636605/1‑94 (MQ=60)
                                                                                                 GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATTTCAACGA   >  SRR4302540.7999617/1‑94 (MQ=51)
                                                                                                  GCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  <  SRR4302540.7161716/94‑1 (MQ=60)
                                                                                                  |                                                                                             
TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑TGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603692‑4603878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑