Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,785	A→G	noncoding (71/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,785	0	A	G	98.8%	489.2 / ‑4.5	176	noncoding (71/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (97/74); total (98/78)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 199 total aligned reads displayed.

CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603697‑4603874

ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTg                                                                                       >  1:3159587/1‑93 (MQ=255)

     aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcc                                                                                   >  1:1741595/1‑93 (MQ=37)

      gCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAAcccc                                                                                 <  1:1662271/93‑1 (MQ=255)

      gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAccc                                                                                  <  2:1490658/93‑1 (MQ=38)

       cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcccc                                                                                 <  1:4618449/93‑1 (MQ=38)

       cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcccc                                                                                 >  1:4103334/1‑93 (MQ=255)

        tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCa                                                                               <  2:6117242/93‑1 (MQ=255)

         aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCa                                                                               <  2:1531837/93‑1 (MQ=38)

          gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCAc                                                                              >  2:2748960/1‑93 (MQ=255)

           cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACg                                                                             <  2:5012346/93‑1 (MQ=38)

            gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGt                                                                            >  1:3551001/1‑93 (MQ=255)

            ctgTCTACCAATTCCGCCACCTTGACACAGTCATCTTACTTTTTTTGATACCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTc                                                                           <  2:1238115/91‑1 (MQ=37)

            ccgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTc                                                                           <  2:4676407/92‑1 (MQ=255)

             cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGGGCTTGAACCCCCACGTc                                                                           <  2:2932993/93‑1 (MQ=255)

              gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          >  1:5885707/1‑93 (MQ=255)

              gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          >  1:2700141/1‑93 (MQ=255)

              gTCTACCAATCCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCGGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          <  2:4797330/93‑1 (MQ=37)

                cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGG‑‑CTTGAACCCCCACGTCCGTaa                                                                      >  1:4125906/1‑93 (MQ=255)

                  aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTaa                                                                      >  2:3071124/1‑93 (MQ=255)

                   ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAg                                                                     >  1:4434944/1‑93 (MQ=37)

                   ccAAGTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAg                                                                     <  2:5928417/93‑1 (MQ=37)

                    cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGG‑CTTGAACCCCCACGGCCCTAAg                                                                     >  1:2304863/1‑93 (MQ=38)

                    cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCCCGTCCGTAAGa                                                                    >  1:4292414/1‑93 (MQ=38)

                       ttCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAaca                                                                 <  1:1483157/93‑1 (MQ=37)

                       ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATACCGCCTCGTTTGGG‑GG‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAaca                                                                 <  2:5719590/93‑1 (MQ=37)

                          cGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTa                                                              <  1:5742140/93‑1 (MQ=25)

                           gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAac                                                            >  1:2903099/1‑93 (MQ=255)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAAGACTAacc                                                           >  1:1255363/1‑92 (MQ=37)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACCCTaaac                                                           >  2:3983699/1‑91 (MQ=37)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:618477/1‑93 (MQ=255)

                               ccTTCGCACATTCATCTTATTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         <  2:4565708/93‑1 (MQ=25)

                               ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAAAACAATAACAcc                                                         >  2:1958508/1‑93 (MQ=37)

                                cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:2817085/1‑93 (MQ=255)

                                 ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  1:6255787/1‑93 (MQ=255)

                                 ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  2:5781135/1‑93 (MQ=255)

                                  tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGa                                                      >  1:5803056/1‑93 (MQ=25)

                                  tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  1:4031510/93‑1 (MQ=255)

                                   cGCACAGTCTTCTTTTTTTTTTTGATATCGCTTCTTTGGGG‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  1:1203076/93‑1 (MQ=18)

                                   cGCACAGTCATCTTATTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  2:4454077/93‑1 (MQ=25)

                                     cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                   >  2:1919608/1‑93 (MQ=255)

                                      acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGCCCGTAAAAACACTAAAACCTGAAGCt                                                  >  2:413668/1‑93 (MQ=25)

                                      acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:5159062/93‑1 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:3180994/93‑1 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 >  1:3203491/1‑93 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 >  2:3527407/1‑93 (MQ=255)

                                        aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                <  1:817040/93‑1 (MQ=255)

                                         tgtaTCTTACTTTTTTTAATATCGCCTGGTTGGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:3304109/90‑1 (MQ=18)

                                         gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:1921583/93‑1 (MQ=255)

                                         gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  2:4264013/93‑1 (MQ=21)

                                         gTCATCTTACTTTTTTCGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:273636/93‑1 (MQ=21)

                                          tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                              >  1:2204169/1‑93 (MQ=21)

                                            aTCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                            >  2:5502572/1‑93 (MQ=255)

                                            aTCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGCCCGTAAGAAAACTAACACCTGAAGCTAGcgcg                                            >  2:5275043/1‑93 (MQ=21)

                                             tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                           >  1:6177298/1‑93 (MQ=255)

                                              cTTACTTTTTTTTATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         <  1:139000/93‑1 (MQ=255)

                                              cTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:2609320/93‑1 (MQ=21)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  1:2284638/1‑93 (MQ=255)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  2:5951047/1‑93 (MQ=255)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        >  1:6213772/1‑93 (MQ=255)

                                               ttACTTTTTTATATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGT‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         <  1:4198125/93‑1 (MQ=17)

                                                 ttgttttttGATACCGCCTCGTTGGGG‑GG‑GAGGGGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        <  2:432646/90‑1 (MQ=14)

                                                 aCTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       >  2:1704337/1‑93 (MQ=255)

                                                   tttttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     >  1:3926931/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  1:473157/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  2:3149510/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   <  1:3774028/93‑1 (MQ=255)

                                                      ttttGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 >  2:2126853/1‑93 (MQ=255)

                                                        ttGATAGCTCCTCGTGTGGTGGG‑GC‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:2579740/93‑1 (MQ=14)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                               >  1:3075200/1‑92 (MQ=17)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:1208031/1‑93 (MQ=255)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               <  1:718356/93‑1 (MQ=255)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:3589717/1‑93 (MQ=255)

                                                          gATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              <  2:3682494/93‑1 (MQ=255)

                                                          gATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              >  1:512491/1‑93 (MQ=255)

                                                           atatCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  1:3257421/1‑93 (MQ=17)

                                                           atatCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  2:5462849/1‑93 (MQ=255)

                                                            aagcgccgcGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                            <  2:620456/90‑1 (MQ=14)

                                                             tgccccTCTTTGGGT‑GG‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           <  2:3459229/89‑1 (MQ=11)

                                                             atCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  1:3659702/1‑93 (MQ=255)

                                                              tCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCCCGTCTACCAATTCCGCCAcc                          >  2:821100/1‑93 (MQ=14)

                                                               cGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  1:3737849/1‑93 (MQ=255)

                                                               cGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  1:6093550/93‑1 (MQ=14)

                                                                gCCTCGTTTGGG‑GC‑GAGGGGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  2:6283318/93‑1 (MQ=14)

                                                                 gcTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       <  1:1878041/92‑1 (MQ=14)

                                                                 ccTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      <  2:5139746/93‑1 (MQ=255)

                                                                 ccTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  1:4769310/1‑93 (MQ=255)

                                                                 ccTCGTTTGGC‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTc                       >  1:1074392/1‑93 (MQ=14)

                                                                  cTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  1:5163771/1‑93 (MQ=255)

                                                                    cGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                    >  1:959614/1‑93 (MQ=255)

                                                                     gTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                   >  2:5559125/1‑93 (MQ=255)

                                                                     cttgggT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  1:5410343/89‑1 (MQ=255)

                                                                      tttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  2:4907933/93‑1 (MQ=11)

                                                                      tttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCa                  >  2:2627145/1‑93 (MQ=11)

                                                                       ttGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  2:1840166/93‑1 (MQ=11)

                                                                       ttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  2:3906980/93‑1 (MQ=255)

                                                                         ggT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCCGTg               >  1:4840528/1‑93 (MQ=11)

                                                                           t‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg             >  2:2962243/1‑93 (MQ=255)

                                                                           t‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg             <  2:748194/93‑1 (MQ=255)

                                                                                ggagggggggggC‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg           <  2:363390/89‑1 (MQ=14)

                                                                                         gggA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa  <  2:3701162/93‑1 (MQ=255)

CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603697‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603692‑4603878

TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGG                                                                                               <  SRR4302533.3123987/94‑1 (MQ=60)

TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGG                                                                                               >  SRR4302533.3804432/1‑94 (MQ=60)

    ACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTG                                                                                           >  SRR4302533.3179031/1‑94 (MQ=60)

     CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGA                                                                                          >  SRR4302533.1565587/1‑94 (MQ=60)

        GAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAC                                                                                        >  SRR4302533.4180036/1‑94 (MQ=60)

        GAAGCGAGCGCGTCTACCAATCCCGCCCCCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAC                                                                                        <  SRR4302533.3496514/94‑1 (MQ=60)

         AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                       >  SRR4302533.226871/1‑94 (MQ=60)

         AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCC                                                                                      <  SRR4302533.3121078/94‑1 (MQ=60)

         AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                       >  SRR4302533.1752157/1‑94 (MQ=60)

         AAGCTAGCGCGTCTACCAATTCAGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                       <  SRR4302533.5891245/94‑1 (MQ=60)

           GCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCC                                                                                    <  SRR4302533.1672320/94‑1 (MQ=60)

           GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                     <  SRR4302533.2605961/94‑1 (MQ=60)

           GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                     >  SRR4302533.4128296/1‑94 (MQ=60)

           GCTAGCGCGTCTACCAAATCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                     >  SRR4302533.1851731/1‑94 (MQ=60)

            CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                    >  SRR4302533.1477028/1‑94 (MQ=60)

            CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                    <  SRR4302533.4646437/94‑1 (MQ=60)

            CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTCC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                    <  SRR4302533.2728603/94‑1 (MQ=60)

               GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACG                                                                                 >  SRR4302533.3832724/1‑94 (MQ=60)

               GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACG                                                                                 <  SRR4302533.2314062/94‑1 (MQ=60)

                CGCGTTTACCAATTCCGCCCCCTTCGCCCAGTCATCTT‑CCTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTC                                                                               <  SRR4302533.3236131/94‑1 (MQ=60)

                CGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGT                                                                                >  SRR4302533.3572876/1‑94 (MQ=60)

                  CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302533.5921271/1‑94 (MQ=60)

                  CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302533.2716707/1‑94 (MQ=60)

                   GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCG                                                                             >  SRR4302533.5923560/1‑94 (MQ=60)

                    TCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAA                                                                          >  SRR4302533.4150951/1‑94 (MQ=60)

                       ACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAG                                                                         >  SRR4302533.4461753/1‑94 (MQ=60)

                        CCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGCTTGAACCCCCACGGCCCTAAG                                                                         >  SRR4302533.2319140/1‑94 (MQ=60)

                        CCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCCCGTCCGTAAGA                                                                        >  SRR4302533.4318229/1‑94 (MQ=60)

                         CAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAA                                                                       <  SRR4302533.426033/94‑1 (MQ=60)

                         CAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAA                                                                       >  SRR4302533.6238274/1‑94 (MQ=60)

                          AATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                      >  SRR4302533.2486604/1‑94 (MQ=60)

                            TTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302533.1492111/94‑1 (MQ=60)

                              CCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                 >  SRR4302533.4900549/1‑94 (MQ=60)

                               CGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                 <  SRR4302533.5776790/94‑1 (MQ=60)

                               CGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                >  SRR4302533.2920929/1‑94 (MQ=60)

                                ccctcctcCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302533.4075527/86‑1 (MQ=60)

                                GCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                >  SRR4302533.4007973/1‑94 (MQ=60)

                                GCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGCACACTAAC                                                                >  SRR4302533.440599/1‑94 (MQ=60)

                                 CCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAGACTAACC                                                               >  SRR4302533.1262868/1‑94 (MQ=60)

                                 CCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               >  SRR4302533.5179003/1‑94 (MQ=60)

                                  CACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAC                                                              <  SRR4302533.4143238/94‑1 (MQ=60)

                                    CCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302533.2834426/1‑94 (MQ=60)

                                     cctccctgtgcgtttttttttttttttggtatccgctttgtttggtgcgggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             <  SRR4302533.5561452/43‑1 (MQ=21)

                                     CTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTG                                                           >  SRR4302533.6293197/1‑94 (MQ=60)

                                      TTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGA                                                          >  SRR4302533.5838107/1‑94 (MQ=60)

                                       TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAA                                                         <  SRR4302533.4056050/94‑1 (MQ=60)

                                       TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        >  SRR4302533.741015/1‑94 (MQ=60)

                                        CGCACAGTCTTCTTTTTTTTTTTGATATCGCTTCTTTGGGGGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302533.1210307/94‑1 (MQ=36)

                                          caaactgtgatgttattttttttaatagcgccgtgttgggtgcgagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302533.57941/48‑1 (MQ=22)

                                          CACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      >  SRR4302533.6317795/1‑94 (MQ=60)

                                           ACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                     >  SRR4302533.3223207/1‑94 (MQ=60)

                                           ACAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                     <  SRR4302533.6252184/94‑1 (MQ=60)

                                            CAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAATCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                    >  SRR4302533.2869098/1‑94 (MQ=60)

                                             AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGCTTGAACCCCCACGCCCGTAAAAACACTAAAACCTGAAGCTAC                                                    >  SRR4302533.1751349/1‑94 (MQ=60)

                                             AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   <  SRR4302533.821827/94‑1 (MQ=60)

                                              tgtATCTTACTTTTTTTAATATCGCCTGGTTGGGTGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  <  SRR4302533.3324513/91‑1 (MQ=37)

                                              GTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  <  SRR4302533.1933312/94‑1 (MQ=60)

                                              GTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  >  SRR4302533.2217754/1‑94 (MQ=60)

                                              GTCATCTTACTTTTTTCGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  <  SRR4302533.275300/94‑1 (MQ=60)

                                                 ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302533.4769495/1‑94 (MQ=60)

                                                 ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302533.6214409/1‑94 (MQ=60)

                                                 ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302533.3742242/1‑94 (MQ=60)

                                                  tttttttttttttttattcccccttttttgggggggggGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCG                                                <  SRR4302533.20491/56‑1 (MQ=8)

                                                   CTTACTTTTTTTTATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                            <  SRR4302533.139884/94‑1 (MQ=60)

                                                   CTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             <  SRR4302533.2625340/94‑1 (MQ=58)

                                                   CTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             >  SRR4302533.2298777/1‑94 (MQ=60)

                                                   CTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                            >  SRR4302533.6251031/1‑94 (MQ=60)

                                                    TTACTTTTTTATATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                            <  SRR4302533.4223535/94‑1 (MQ=43)

                                                     TACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                           <  SRR4302533.1365887/94‑1 (MQ=60)

                                                       CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCA                                         >  SRR4302533.3950904/1‑94 (MQ=59)

                                                         TTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       <  SRR4302533.218350/94‑1 (MQ=59)

                                                         TTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       >  SRR4302533.475986/1‑94 (MQ=54)

                                                          tttttttaaattcgcctgttttggggggggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                        <  SRR4302533.6323083/64‑1 (MQ=9)

                                                          TTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                      <  SRR4302533.3797162/94‑1 (MQ=56)

                                                           TTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                     >  SRR4302533.2943553/1‑94 (MQ=52)

                                                             TTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCC                                   >  SRR4302533.3094073/1‑94 (MQ=47)

                                                              TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                  >  SRR4302533.515536/1‑94 (MQ=45)

                                                              TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                  >  SRR4302533.5839285/1‑94 (MQ=45)

                                                              TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                  <  SRR4302533.722596/94‑1 (MQ=42)

                                                               ttgatagctcctcgtgtggtggggcGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                   <  SRR4302533.2595615/69‑1 (MQ=8)

                                                               GATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGCC                                 >  SRR4302533.3277510/1‑94 (MQ=38)

                                                               GATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC                                 >  SRR4302533.5257842/1‑94 (MQ=42)

                                                                ATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGGTTGAACCCCCCCGCCCGAAAGAACACTAAACCCTGAAGCTAGCGCGTCTACCAATTtcccc                                 >  SRR4302533.3592524/1‑89 (MQ=28)

                                                                ATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                                >  SRR4302533.3279795/1‑94 (MQ=40)

                                                                 TATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               >  SRR4302533.3682206/1‑94 (MQ=38)

                                                                   TCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302533.1254066/1‑94 (MQ=31)

                                                                   TCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            >  SRR4302533.3760775/1‑94 (MQ=35)

                                                                    CTCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            >  SRR4302533.99102/1‑94 (MQ=25)

                                                                    CGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            >  SRR4302533.3180724/1‑94 (MQ=30)

                                                                    CGCCTCGTTTGGGGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                            <  SRR4302533.6130370/94‑1 (MQ=22)

                                                                     GCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          >  SRR4302533.4798217/1‑94 (MQ=30)

                                                                     GCCTCGTTTGGCGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTC                           >  SRR4302533.1080725/1‑94 (MQ=19)

                                                                      GCTCGTTTGGGGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302533.1889487/94‑1 (MQ=10)

                                                                      CCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          >  SRR4302533.5194950/1‑94 (MQ=23)

                                                                       CTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGC                         >  SRR4302533.578388/1‑94 (MQ=19)

                                                                       CTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302533.4618036/1‑94 (MQ=21)

                                                                        gctgtttgtgtgggagGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302533.228447/78‑1 (MQ=7)

                                                                        TCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                        >  SRR4302533.965259/1‑94 (MQ=21)

                                                                         CGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302533.3768326/1‑94 (MQ=21)

                                                                         CGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGACCGTAAAGACACTAACACCAGAAGCTAGGGCGTCTACCAATTGCGCCACCTTCGCCC                       >  SRR4302533.5306146/1‑94 (MQ=57)

                                                                          cttgGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302533.5442871/90‑1 (MQ=23)

                                                                          GTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCA                      >  SRR4302533.6152809/1‑94 (MQ=21)

                                                                           TTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     <  SRR4302533.1932027/94‑1 (MQ=21)

                                                                           TTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     >  SRR4302533.5197325/1‑94 (MQ=21)

                                                                             TGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTG                   <  SRR4302533.6127574/94‑1 (MQ=26)

                                                                             TGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCCGTG                   >  SRR4302533.4869867/1‑94 (MQ=5)

                                                                              ttggggggagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGT                    <  SRR4302533.1268247/84‑1 (MQ=26)

                                                                               GTGC‑GG‑‑GTGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGA                <  SRR4302533.486799/94‑1 (MQ=31)

                                                                                TGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTTGCCCAGTGCGA                >  SRR4302533.2122093/1‑94 (MQ=27)

                                                                                 gggcgagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG                 <  SRR4302533.3640568/87‑1 (MQ=34)

                                                                                     G‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302533.3586366/94‑1 (MQ=47)

                                                                                     G‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAAT           >  SRR4302533.2812324/1‑94 (MQ=33)

                                                                                         GGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         <  SRR4302533.4726853/94‑1 (MQ=51)

                                                                                         GGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         <  SRR4302533.873281/94‑1 (MQ=53)

                                                                                         GGGGGGGGCTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT         <  SRR4302533.342901/94‑1 (MQ=51)

                                                                                           GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCA       <  SRR4302533.2668180/94‑1 (MQ=58)

                                                                                            GGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA      >  SRR4302533.5709151/1‑94 (MQ=60)

                                                                                             GGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAC     <  SRR4302533.2416894/94‑1 (MQ=60)

                                                                                             GGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAC     >  SRR4302533.38712/1‑94 (MQ=60)

                                                                                              GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    <  SRR4302533.2599919/94‑1 (MQ=60)

                                                                                              GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    >  SRR4302533.5253444/1‑94 (MQ=60)

                                                                                              GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG    >  SRR4302533.3525723/1‑94 (MQ=60)

                                                                                               GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT   >  SRR4302533.5713334/1‑94 (MQ=60)

                                                                                               GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT   >  SRR4302533.2621594/1‑94 (MQ=60)

                                                                                                GCTTGACCCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  <  SRR4302533.3409893/94‑1 (MQ=60)

                                                                                                GCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  <  SRR4302533.3417182/94‑1 (MQ=60)

TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603692‑4603878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`